Lotus
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0199.8
         (105 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YC22_ARATH (O81916) Hypothetical calcium-binding protein At1g02270    121  3e-28
CYSK_BUCAP (Q8KA48) Cysteine synthase (EC 2.5.1.47) (O-acetylser...    27  6.6
ARH8_MOUSE (Q9Z206) Neuroepithelial cell transforming gene 1 pro...    27  8.6
ARH8_HUMAN (Q7Z628) Neuroepithelial cell transforming gene 1 pro...    27  8.6

>YC22_ARATH (O81916) Hypothetical calcium-binding protein At1g02270
          Length = 232

 Score =  121 bits (304), Expect = 3e-28
 Identities = 58/86 (67%), Positives = 68/86 (78%), Gaps = 2/86 (2%)

Query: 20  MSIVLCSDWNGSKLGHVYKFLRTQRFDSSYDTANKYIDSYADLHK*VSHRNPRGNICGVD 79
           M I+LC DWNGSK GHVYKFLR+Q F SSYD A++Y DS  D H+ VSHRN RGNICGVD
Sbjct: 1   MPIILCGDWNGSKRGHVYKFLRSQGFISSYDDAHQYTDS--DAHRWVSHRNHRGNICGVD 58

Query: 80  FICIFNPNQALKPLKTSWAEAVLTII 105
           FI + NP+ + KPL+TSW EAV +II
Sbjct: 59  FIWLCNPSDSRKPLRTSWVEAVFSII 84


>CYSK_BUCAP (Q8KA48) Cysteine synthase (EC 2.5.1.47) (O-acetylserine
           sulfhydrylase) (O-acetylserine (Thiol)-lyase) (CSase)
          Length = 322

 Score = 27.3 bits (59), Expect = 6.6
 Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 6/44 (13%)

Query: 12  QKENRFKSMSIVLCSDWNGSKLGHVYKFLRTQRFDSSYDTANKY 55
           QKEN FK+ +IV+    +G       ++L T+ F  S+   N Y
Sbjct: 285 QKENDFKNKNIVVILPSSGE------RYLSTELFSRSFKNENNY 322


>ARH8_MOUSE (Q9Z206) Neuroepithelial cell transforming gene 1
           protein (Rho guanine nucleotide exchange factor 8)
          Length = 595

 Score = 26.9 bits (58), Expect = 8.6
 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 1/37 (2%)

Query: 5   LQYVESYQKENRFKSMSIVLCSDWNGSKLGH-VYKFL 40
           L+Y++  QK+ R ++  ++LC     +K GH +Y FL
Sbjct: 369 LEYLDEKQKDPRIEASKVLLCHGELKNKSGHKLYIFL 405


>ARH8_HUMAN (Q7Z628) Neuroepithelial cell transforming gene 1
           protein (p65 Net1 proto-oncogene) (Rho guanine
           nucleotide exchange factor 8)
          Length = 596

 Score = 26.9 bits (58), Expect = 8.6
 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 1/37 (2%)

Query: 5   LQYVESYQKENRFKSMSIVLCSDWNGSKLGH-VYKFL 40
           L+Y++  Q++ R ++  ++LC     SK GH +Y FL
Sbjct: 369 LEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFL 405


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.327    0.139    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,950,452
Number of Sequences: 164201
Number of extensions: 411940
Number of successful extensions: 742
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 740
Number of HSP's gapped (non-prelim): 4
length of query: 105
length of database: 59,974,054
effective HSP length: 81
effective length of query: 24
effective length of database: 46,673,773
effective search space: 1120170552
effective search space used: 1120170552
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 58 (26.9 bits)


Lotus: description of TM0199.8