BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0199.2
(160 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RPB1_PLAFD (P14248) DNA-directed RNA polymerase II largest subun... 32 0.80
G3P_COLGL (P35143) Glyceraldehyde-3-phosphate dehydrogenase (EC ... 32 0.80
MSC1_YEAST (Q03104) Meiotic sister chromatid recombination prote... 31 1.0
G3P_MONAN (P53430) Glyceraldehyde-3-phosphate dehydrogenase (EC ... 30 1.8
G3P_COLLN (P54117) Glyceraldehyde-3-phosphate dehydrogenase (EC ... 30 1.8
YKK5_YEAST (P34250) Hypothetical 125.6 kDa protein in AAT1-GFA1 ... 30 2.3
G3P_CURLU (P28844) Glyceraldehyde-3-phosphate dehydrogenase (EC ... 30 2.3
CH60_PLAFG (P34940) Chaperonin CPN60, mitochondrial precursor 30 2.3
G3P_DROHY (Q01597) Glyceraldehyde-3-phosphate dehydrogenase (EC ... 29 5.2
G3P_ASPNG (Q12552) Glyceraldehyde-3-phosphate dehydrogenase (EC ... 29 5.2
G3P2_DROSU (O44105) Glyceraldehyde-3-phosphate dehydrogenase II ... 29 5.2
G3P2_DROPS (O44104) Glyceraldehyde-3-phosphate dehydrogenase II ... 29 5.2
G3P2_DROME (P07487) Glyceraldehyde-3-phosphate dehydrogenase II ... 29 5.2
YQ38_CAEEL (Q09459) Hypothetical protein C09G5.8 in chromosome II 28 6.8
YAH3_YEAST (P39706) Hypothetical 48.7 kDa Trp-Asp repeats contai... 28 6.8
SIS2_YEAST (P36024) SIS2 protein (Halotolerance protein HAL3) 28 6.8
CRTC_HUMAN (P27797) Calreticulin precursor (CRP55) (Calregulin) ... 28 6.8
BTUB_SALTY (P37409) Vitamin B12 receptor precursor 28 6.8
RPO7_FOWPV (Q05569) DNA-directed RNA polymerase 19 kDa polypepti... 28 8.9
LX21_HORVU (P93184) Lipoxygenase 2.1, chloroplast precursor (EC ... 28 8.9
>RPB1_PLAFD (P14248) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6)
Length = 2452
Score = 31.6 bits (70), Expect = 0.80
Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 2/53 (3%)
Query: 110 PADKKWSMVQLSNKINDNKDYDENQQKSVD--KEDDPFKGISHSLDDDLIEDD 160
PA S VQ+ + D + QQ VD K DDPF + +++D+D ++++
Sbjct: 2399 PAYILQSPVQIKQNVQDVNMFSPIQQAHVDEAKNDDPFSPMPYNIDEDEMKEN 2451
>G3P_COLGL (P35143) Glyceraldehyde-3-phosphate dehydrogenase (EC
1.2.1.12) (GAPDH)
Length = 338
Score = 31.6 bits (70), Expect = 0.80
Identities = 14/45 (31%), Positives = 24/45 (53%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ +I YDE +Q + + P KG+ +DD++ D
Sbjct: 241 SVVDLTARIEKGASYDEIKQAIKEAAEGPLKGVLAYTEDDVVSTD 285
>MSC1_YEAST (Q03104) Meiotic sister chromatid recombination protein
1
Length = 513
Score = 31.2 bits (69), Expect = 1.0
Identities = 22/83 (26%), Positives = 38/83 (45%), Gaps = 5/83 (6%)
Query: 71 NGVRIDDLGFTQVDLNRVGYKNDPFILASQAEQVFFVTDPADKKWSMVQLSNKINDNK-D 129
NGV +DD ++ L + +N I S ++ + WS L N +NDN D
Sbjct: 124 NGVDVDDAKASKDSLVKTAKENFNKISKSLKSSGYYPSSSYFDSWSTKDLQNWLNDNGID 183
Query: 130 YDENQQKSVDKEDDPFKGISHSL 152
YD K+V +D+ + + ++
Sbjct: 184 YD----KAVQSKDELVQKVKENI 202
>G3P_MONAN (P53430) Glyceraldehyde-3-phosphate dehydrogenase (EC
1.2.1.12) (GAPDH)
Length = 337
Score = 30.4 bits (67), Expect = 1.8
Identities = 13/45 (28%), Positives = 24/45 (52%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ ++ YDE +Q + + P KG+ +DD++ D
Sbjct: 241 SVVDLTARLEKAATYDEIKQAVKEASEGPLKGVLGYTEDDVVSSD 285
>G3P_COLLN (P54117) Glyceraldehyde-3-phosphate dehydrogenase (EC
1.2.1.12) (GAPDH)
Length = 337
Score = 30.4 bits (67), Expect = 1.8
Identities = 14/45 (31%), Positives = 23/45 (51%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ +I YDE +Q + + P KG+ +DD + D
Sbjct: 240 SVVDLTVRIEKGATYDEIKQAIKEAAEGPLKGVLAYTEDDFVSTD 284
>YKK5_YEAST (P34250) Hypothetical 125.6 kDa protein in AAT1-GFA1
intergenic region
Length = 1132
Score = 30.0 bits (66), Expect = 2.3
Identities = 13/49 (26%), Positives = 28/49 (56%)
Query: 112 DKKWSMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
D+ S++ +N N++KD D+++ D +DD ++ D+ ++DD
Sbjct: 579 DETGSVMDKNNGPNNDKDDDDDKDNDNDDDDDKDDDVNDDDKDENVDDD 627
>G3P_CURLU (P28844) Glyceraldehyde-3-phosphate dehydrogenase (EC
1.2.1.12) (GAPDH)
Length = 337
Score = 30.0 bits (66), Expect = 2.3
Identities = 14/45 (31%), Positives = 23/45 (51%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ +I YDE +Q + + P GI +DD++ D
Sbjct: 240 SVVDLTVRIEKGASYDEIKQAVKEASEGPLSGILGYTEDDIVTTD 284
>CH60_PLAFG (P34940) Chaperonin CPN60, mitochondrial precursor
Length = 700
Score = 30.0 bits (66), Expect = 2.3
Identities = 14/39 (35%), Positives = 24/39 (60%), Gaps = 5/39 (12%)
Query: 122 NKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
+K++D++D DE+ ++ D EDD LDDD +D+
Sbjct: 639 HKLHDDEDTDEDDEEDEDDEDD-----EDDLDDDDYDDE 672
>G3P_DROHY (Q01597) Glyceraldehyde-3-phosphate dehydrogenase (EC
1.2.1.12) (GAPDH)
Length = 332
Score = 28.9 bits (63), Expect = 5.2
Identities = 13/45 (28%), Positives = 24/45 (52%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ ++ YDE + K + + P KGI D++++ D
Sbjct: 238 SVVDLTVRLGKGASYDEIKAKVQEAANGPLKGILGYTDEEVVSTD 282
>G3P_ASPNG (Q12552) Glyceraldehyde-3-phosphate dehydrogenase (EC
1.2.1.12) (GAPDH)
Length = 336
Score = 28.9 bits (63), Expect = 5.2
Identities = 13/45 (28%), Positives = 24/45 (52%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ ++ YDE ++ D ++ KGI +DD++ D
Sbjct: 240 SVVDLTCRLEKATSYDEIKKALKDASENELKGILGYTEDDIVSSD 284
>G3P2_DROSU (O44105) Glyceraldehyde-3-phosphate dehydrogenase II (EC
1.2.1.12) (GAPDH II) (Fragment)
Length = 304
Score = 28.9 bits (63), Expect = 5.2
Identities = 13/45 (28%), Positives = 24/45 (52%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ ++ YDE + K + + P KGI D++++ D
Sbjct: 228 SVVDLTVRLGKGASYDEIKAKVQEAANGPLKGILGYTDEEVVSTD 272
>G3P2_DROPS (O44104) Glyceraldehyde-3-phosphate dehydrogenase II (EC
1.2.1.12) (GAPDH II) (Fragment)
Length = 304
Score = 28.9 bits (63), Expect = 5.2
Identities = 13/45 (28%), Positives = 24/45 (52%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ ++ YDE + K + + P KGI D++++ D
Sbjct: 228 SVVDLTVRLGKGASYDEIKAKVQEAANGPLKGILGYTDEEVVSTD 272
>G3P2_DROME (P07487) Glyceraldehyde-3-phosphate dehydrogenase II (EC
1.2.1.12) (GAPDH II)
Length = 332
Score = 28.9 bits (63), Expect = 5.2
Identities = 13/45 (28%), Positives = 24/45 (52%)
Query: 116 SMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
S+V L+ ++ YDE + K + + P KGI D++++ D
Sbjct: 238 SVVDLTVRLGKGASYDEIKAKVQEAANGPLKGILGYTDEEVVSTD 282
>YQ38_CAEEL (Q09459) Hypothetical protein C09G5.8 in chromosome II
Length = 1531
Score = 28.5 bits (62), Expect = 6.8
Identities = 25/93 (26%), Positives = 39/93 (41%), Gaps = 6/93 (6%)
Query: 57 FQVPVFGCRWVDNNNGVRIDDLGFTQVD-----LNRVGYKNDPFILASQAEQVFFVTDPA 111
+++P VD++ D + F D ++ V + DP L QV + P
Sbjct: 1126 YEIPAVTPALVDSDGEEEADRIVFDDDDDEIESVSAVSSQRDPEPLEVPERQVENLPSPE 1185
Query: 112 DKKWSMVQLS-NKINDNKDYDENQQKSVDKEDD 143
D L N N++K+ Q+SVDK DD
Sbjct: 1186 DTPRPSDPLKPNGTNESKESTPVTQRSVDKTDD 1218
>YAH3_YEAST (P39706) Hypothetical 48.7 kDa Trp-Asp repeats
containing protein in TFC3-RFA1 intergenic region
Length = 426
Score = 28.5 bits (62), Expect = 6.8
Identities = 11/38 (28%), Positives = 21/38 (54%), Gaps = 1/38 (2%)
Query: 57 FQVPVFGCRWVDNNNGVRIDDLGFTQVDLNRVGYKNDP 94
F P++GC+W+D + + + F + D + + NDP
Sbjct: 113 FDSPIWGCQWLDAKRRLCVATI-FEESDAYVIDFSNDP 149
>SIS2_YEAST (P36024) SIS2 protein (Halotolerance protein HAL3)
Length = 562
Score = 28.5 bits (62), Expect = 6.8
Identities = 13/38 (34%), Positives = 24/38 (62%)
Query: 123 KINDNKDYDENQQKSVDKEDDPFKGISHSLDDDLIEDD 160
K N+ +D DE++++ D+E+D ++ DDD +DD
Sbjct: 493 KNNEEEDDDEDEEEDDDEEEDTEDKNENNNDDDDDDDD 530
>CRTC_HUMAN (P27797) Calreticulin precursor (CRP55) (Calregulin)
(HACBP) (ERp60) (grp60)
Length = 417
Score = 28.5 bits (62), Expect = 6.8
Identities = 15/57 (26%), Positives = 28/57 (48%)
Query: 100 QAEQVFFVTDPADKKWSMVQLSNKINDNKDYDENQQKSVDKEDDPFKGISHSLDDDL 156
Q E+ + DKK + + D++D DE+++ DKE+D + + D+L
Sbjct: 361 QDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL 417
>BTUB_SALTY (P37409) Vitamin B12 receptor precursor
Length = 614
Score = 28.5 bits (62), Expect = 6.8
Identities = 20/69 (28%), Positives = 32/69 (45%), Gaps = 2/69 (2%)
Query: 89 GYKNDPFILASQAEQV--FFVTDPADKKWSMVQLSNKINDNKDYDENQQKSVDKEDDPFK 146
GY +P + +++Q F A W + N IND DYD++ QK ++ K
Sbjct: 423 GYYGNPNLNPEKSKQWEGAFEGLTAGVSWRISGYRNDINDMIDYDDHLQKYYNEGKARIK 482
Query: 147 GISHSLDDD 155
GI + + D
Sbjct: 483 GIEATANFD 491
>RPO7_FOWPV (Q05569) DNA-directed RNA polymerase 19 kDa polypeptide
(EC 2.7.7.6)
Length = 167
Score = 28.1 bits (61), Expect = 8.9
Identities = 14/34 (41%), Positives = 18/34 (52%)
Query: 121 SNKINDNKDYDENQQKSVDKEDDPFKGISHSLDD 154
S IND D+N KS D++DD I + DD
Sbjct: 4 SMDINDILLSDDNDYKSYDEDDDSISDIGETSDD 37
>LX21_HORVU (P93184) Lipoxygenase 2.1, chloroplast precursor (EC
1.13.11.12) (LOX-100) (LOX2:Hv:1)
Length = 936
Score = 28.1 bits (61), Expect = 8.9
Identities = 9/43 (20%), Positives = 26/43 (59%)
Query: 18 VTLIAQSMHISSAKDKKPVHGSMSYFGVIELIWELDYTIFQVP 60
V L++ + + ++++P+ G++ + G ++ W++ F+VP
Sbjct: 117 VDLVSSELDPQTGQEREPISGAVKHSGRVDDEWDMYEATFKVP 159
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.135 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,749,272
Number of Sequences: 164201
Number of extensions: 791187
Number of successful extensions: 2339
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 2297
Number of HSP's gapped (non-prelim): 37
length of query: 160
length of database: 59,974,054
effective HSP length: 101
effective length of query: 59
effective length of database: 43,389,753
effective search space: 2559995427
effective search space used: 2559995427
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0199.2