BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0192.7
(263 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NFM_RAT (P12839) Neurofilament triplet M protein (160 kDa neurof... 33 0.54
MYH7_MESAU (P13540) Myosin heavy chain, cardiac muscle beta isof... 33 0.54
MYH7_RAT (P02564) Myosin heavy chain, cardiac muscle beta isofor... 33 0.70
MYH7_RABIT (P04461) Myosin heavy chain, cardiac muscle beta isof... 33 0.70
MYH7_PIG (P79293) Myosin heavy chain, cardiac muscle beta isofor... 33 0.70
MYS_AEQIR (P24733) Myosin heavy chain, striated muscle 33 0.92
DNAK_STAEP (Q8CP17) Chaperone protein dnaK (Heat shock protein 7... 33 0.92
MYH3_RAT (P12847) Myosin heavy chain, fast skeletal muscle, embr... 32 1.2
MYH7_HUMAN (P12883) Myosin heavy chain, cardiac muscle beta isof... 32 1.6
CENB_MOUSE (P27790) Major centromere autoantigen B (Centromere p... 32 2.1
CAC1_YEAST (Q12495) Chromatin assembly factor 1 p90 subunit (CAF... 32 2.1
MYSS_CYPCA (Q90339) Myosin heavy chain, fast skeletal muscle 31 2.7
CENB_CRIGR (P48988) Major centromere autoantigen B (Centromere p... 31 3.5
NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa neur... 30 4.6
MYSS_CHICK (P13538) Myosin heavy chain, skeletal muscle, adult 30 4.6
SG1_MOUSE (P16014) Secretogranin I precursor (SgI) (Chromogranin... 30 6.0
MYHB_HUMAN (P35749) Myosin heavy chain, smooth muscle isoform (S... 30 6.0
MVIN_THEMA (Q9WXU1) Virulence factor mviN homolog 30 6.0
SYA_PSEAE (Q9I553) Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine-... 30 7.8
RCCR_ARATH (Q8LDU4) Red chlorophyll catabolite reductase, chloro... 30 7.8
>NFM_RAT (P12839) Neurofilament triplet M protein (160 kDa
neurofilament protein) (Neurofilament medium
polypeptide) (NF-M)
Length = 845
Score = 33.5 bits (75), Expect = 0.54
Identities = 32/124 (25%), Positives = 52/124 (41%), Gaps = 27/124 (21%)
Query: 115 RQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPAR--SMEEM 172
+++E K+ VA+ S KVE +E + + K K P + ++EEM
Sbjct: 627 KEEEKVEEKKEVAKESPKEEKVEKKEEKPKDVPDK----------KKAESPVKEKAVEEM 676
Query: 173 RARASTYILDEEDDAFKRKRAKLEK---------------GDTSPEQR*KEEFQIGGKAE 217
+ + E D + K + EK GD SP++ KE+ I G+ E
Sbjct: 677 ITITKSVKVSLEKDTKEEKPQQQEKVKEKAEEEGGSEEEVGDKSPQESKKEDIAINGEVE 736
Query: 218 GKEQ 221
GKE+
Sbjct: 737 GKEE 740
>MYH7_MESAU (P13540) Myosin heavy chain, cardiac muscle beta isoform
(MyHC-beta)
Length = 1934
Score = 33.5 bits (75), Expect = 0.54
Identities = 28/91 (30%), Positives = 47/91 (50%), Gaps = 21/91 (23%)
Query: 107 TINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPA 166
+I DL + +QQ E LK+ +A + ++EDE+ L S+L +K
Sbjct: 1064 SIMDLENDKQQLDEKLKKKDFELNALNARIEDEQ---------------ALGSQLQKK-- 1106
Query: 167 RSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
++E++AR L+EE +A + RAK+EK
Sbjct: 1107 --LKELQARIEE--LEEELEAERTARAKVEK 1133
>MYH7_RAT (P02564) Myosin heavy chain, cardiac muscle beta isoform
(MyHC-beta)
Length = 1935
Score = 33.1 bits (74), Expect = 0.70
Identities = 28/91 (30%), Positives = 47/91 (50%), Gaps = 21/91 (23%)
Query: 107 TINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPA 166
+I DL + +QQ E LK+ +A + ++EDE+ L S+L +K
Sbjct: 1065 SIMDLENDKQQLDERLKKKDFELNALNARIEDEQ---------------ALGSQLQKK-- 1107
Query: 167 RSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
++E++AR L+EE +A + RAK+EK
Sbjct: 1108 --LKELQARIEE--LEEELEAERTARAKVEK 1134
>MYH7_RABIT (P04461) Myosin heavy chain, cardiac muscle beta isoform
(MyHC-beta) (Beta isomyosin) (Fragment)
Length = 736
Score = 33.1 bits (74), Expect = 0.70
Identities = 28/91 (30%), Positives = 47/91 (50%), Gaps = 21/91 (23%)
Query: 107 TINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPA 166
+I DL + +QQ E LK+ +A + ++EDE+ L S+L +K
Sbjct: 629 SIMDLENDKQQLDERLKKKDFELNALNARIEDEQ---------------ALGSQLQKK-- 671
Query: 167 RSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
++E++AR L+EE +A + RAK+EK
Sbjct: 672 --LKELQARIEE--LEEELEAERTARAKVEK 698
>MYH7_PIG (P79293) Myosin heavy chain, cardiac muscle beta isoform
(MyHC-beta)
Length = 1935
Score = 33.1 bits (74), Expect = 0.70
Identities = 28/91 (30%), Positives = 47/91 (50%), Gaps = 21/91 (23%)
Query: 107 TINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPA 166
+I DL + +QQ E LK+ +A + ++EDE+ L S+L +K
Sbjct: 1065 SIMDLENDKQQLDERLKKKDFELNALNARIEDEQ---------------ALGSQLQKK-- 1107
Query: 167 RSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
++E++AR L+EE +A + RAK+EK
Sbjct: 1108 --LKELQARIEE--LEEELEAERTARAKVEK 1134
>MYS_AEQIR (P24733) Myosin heavy chain, striated muscle
Length = 1938
Score = 32.7 bits (73), Expect = 0.92
Identities = 27/96 (28%), Positives = 49/96 (50%), Gaps = 21/96 (21%)
Query: 102 KNQPVTINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKL 161
K+ + DL ++++ E+++ A S+ + K+EDE+ L S+L
Sbjct: 1057 KSTQENVEDLERVKRELEENVRRKEAEISSLNSKLEDEQ---------------NLVSQL 1101
Query: 162 TRKPARSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
RK ++E++AR L+EE +A + RAK+EK
Sbjct: 1102 QRK----IKELQARIEE--LEEELEAERNARAKVEK 1131
>DNAK_STAEP (Q8CP17) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 609
Score = 32.7 bits (73), Expect = 0.92
Identities = 29/126 (23%), Positives = 54/126 (42%), Gaps = 3/126 (2%)
Query: 101 NKNQPVTINDLYSIRQQEGESLKEYVARYSAASVKVEDE---EPRACALAFKNGLLPGGL 157
NK Q +TI S+ +E + + + + A K +E A +L F+ L
Sbjct: 464 NKEQNITIQSSSSLSDEEIDRMVKDAEENAEADKKRREEVDLRNEADSLVFQVEKTVKDL 523
Query: 158 NSKLTRKPARSMEEMRARASTYILDEEDDAFKRKRAKLEKGDTSPEQR*KEEFQIGGKAE 217
++ + ++ EE + T + E+ D K K+ +LEK + E+ Q +
Sbjct: 524 GENISDEDKKNAEEKKDALKTALEGEDIDDIKAKKEELEKVIQELSAKVYEQAQQAQQQA 583
Query: 218 GKEQGA 223
+EQG+
Sbjct: 584 QEEQGS 589
>MYH3_RAT (P12847) Myosin heavy chain, fast skeletal muscle, embryonic
Length = 1940
Score = 32.3 bits (72), Expect = 1.2
Identities = 30/91 (32%), Positives = 45/91 (48%), Gaps = 21/91 (23%)
Query: 107 TINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPA 166
+I DL + +QQ E LK+ YS KVEDE+ L+ +L +K
Sbjct: 1066 SILDLENDKQQLDERLKKKDFEYSQLQSKVEDEQT---------------LSLQLQKK-- 1108
Query: 167 RSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
++E++AR L+EE +A + RAK EK
Sbjct: 1109 --IKELQARIEE--LEEEIEAERATRAKTEK 1135
>MYH7_HUMAN (P12883) Myosin heavy chain, cardiac muscle beta isoform
(MyHC-beta)
Length = 1935
Score = 32.0 bits (71), Expect = 1.6
Identities = 27/91 (29%), Positives = 47/91 (50%), Gaps = 21/91 (23%)
Query: 107 TINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPA 166
+I DL + +QQ E LK+ +A + ++EDE+ L S+L +K
Sbjct: 1065 SIMDLENDKQQLDERLKKKDFELNALNARIEDEQ---------------ALGSQLQKK-- 1107
Query: 167 RSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
++E++AR L+EE ++ + RAK+EK
Sbjct: 1108 --LKELQARIEE--LEEELESERTARAKVEK 1134
>CENB_MOUSE (P27790) Major centromere autoantigen B (Centromere
protein B) (CENP-B)
Length = 599
Score = 31.6 bits (70), Expect = 2.1
Identities = 27/107 (25%), Positives = 45/107 (41%), Gaps = 12/107 (11%)
Query: 140 EPRACALAFKNGLLPGGLNSKLTRKPARSMEEMRARASTYILDEEDDAFKRKRAKLEKGD 199
EP A F+ GGLN+ +T EE EE++ + + + E+G+
Sbjct: 376 EPSDIATCFREAGFGGGLNATITTSFKSEGEE-----------EEEEEEEEEEEEEEEGE 424
Query: 200 TSPEQR*KEEFQIGGKAEGKEQGAFTRE*QIEILQGASSDGRSEQHG 246
E+ +EE + G EG+E+G E + E+ + S G
Sbjct: 425 GEEEEEEEEEGEEEG-GEGEEEGEEEVEEEGEVDDSDEEEEESSSEG 470
>CAC1_YEAST (Q12495) Chromatin assembly factor 1 p90 subunit (CAF-1
90 kDa subunit) (RAP1 localization factor 2)
Length = 606
Score = 31.6 bits (70), Expect = 2.1
Identities = 19/65 (29%), Positives = 34/65 (52%)
Query: 157 LNSKLTRKPARSMEEMRARASTYILDEEDDAFKRKRAKLEKGDTSPEQR*KEEFQIGGKA 216
L + +K +EE R R + +E++ +++ A+LE E+R K+E +I K
Sbjct: 161 LRQEEKKKKELKVEEERQRRAELKKQKEEEKRRKEEARLEAKRRKEEERLKKEEEIRLKE 220
Query: 217 EGKEQ 221
E KE+
Sbjct: 221 EAKER 225
>MYSS_CYPCA (Q90339) Myosin heavy chain, fast skeletal muscle
Length = 1935
Score = 31.2 bits (69), Expect = 2.7
Identities = 27/91 (29%), Positives = 45/91 (48%), Gaps = 21/91 (23%)
Query: 107 TINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPA 166
+I DL + +QQ E +K+ S K+EDE+ L ++L +K
Sbjct: 1066 SIMDLENEKQQSDEKIKKKDFEISQLLSKIEDEQ---------------SLGAQLQKK-- 1108
Query: 167 RSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
++E++AR L+EE +A + RAK+EK
Sbjct: 1109 --IKELQARIEE--LEEEIEAERAARAKVEK 1135
>CENB_CRIGR (P48988) Major centromere autoantigen B (Centromere
protein B) (CENP-B)
Length = 606
Score = 30.8 bits (68), Expect = 3.5
Identities = 28/105 (26%), Positives = 45/105 (42%), Gaps = 16/105 (15%)
Query: 140 EPRACALAFKNGLLPGGLNSKLTRKPARSMEEMRARASTYILDEEDDAFKRKRAKLEKGD 199
EP A F+ GGLN+ +T EE EE++ + + + E+G+
Sbjct: 376 EPADIATCFREAGFGGGLNATITTSFKSEGEE-----------EEEEEEEEEEEEEEEGE 424
Query: 200 TSPEQR*KEEFQIGGKAEGKEQGAFTRE*QIEILQGASSDGRSEQ 244
E+ +EE G+ EG E E ++E +G SD E+
Sbjct: 425 GEEEEEEEEE----GEEEGGEGEEVGEEEEVE-EEGDESDEEEEE 464
>NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa
neurofilament protein) (Neurofilament medium
polypeptide) (NF-M) (Neurofilament 3)
Length = 915
Score = 30.4 bits (67), Expect = 4.6
Identities = 46/205 (22%), Positives = 82/205 (39%), Gaps = 30/205 (14%)
Query: 73 MAWFTTLPRGSISNFRDFSSKFLVQFSANKNQPVTINDLYSIRQQEGESLK---EYVARY 129
+A + L G + F F+ ++ P+TI+ + E LK ++V
Sbjct: 397 IAAYRKLLEGEETRFSTFAGSITGPLYTHR-PPITISSKIQKTKVEAPKLKVQHKFVEEI 455
Query: 130 SAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPARSMEEMRARASTYI-----LDEE 184
KVEDE+ + L + +T + A SM+E + A+ +EE
Sbjct: 456 -IEETKVEDEKSE-----MEEAL------TAITEELAASMKEEKKEAAEEKEEEPEAEEE 503
Query: 185 DDAFKRKRAKL-------EKGDTSPE--QR*KEEFQIGGKAEGKEQGAFTRE*QIEILQG 235
+ A K+ K E+G+ E Q +EE G K++ E+G +E E +G
Sbjct: 504 EVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEG 563
Query: 236 ASSDGRSEQHGSVRQ*RASTEVRLQ 260
+G +E + A E +++
Sbjct: 564 EQEEGETEAEAEGEEAEAKEEKKVE 588
>MYSS_CHICK (P13538) Myosin heavy chain, skeletal muscle, adult
Length = 1938
Score = 30.4 bits (67), Expect = 4.6
Identities = 28/91 (30%), Positives = 43/91 (46%), Gaps = 21/91 (23%)
Query: 107 TINDLYSIRQQEGESLKEYVARYSAASVKVEDEEPRACALAFKNGLLPGGLNSKLTRKPA 166
+I DL + +QQ E LK+ S K+EDE+ L +L +K
Sbjct: 1067 SIMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQ---------------ALGMQLQKK-- 1109
Query: 167 RSMEEMRARASTYILDEEDDAFKRKRAKLEK 197
++E++AR L+EE +A + RAK EK
Sbjct: 1110 --IKELQARIEE--LEEEIEAERTSRAKAEK 1136
>SG1_MOUSE (P16014) Secretogranin I precursor (SgI) (Chromogranin B)
(CgB)
Length = 677
Score = 30.0 bits (66), Expect = 6.0
Identities = 20/83 (24%), Positives = 39/83 (46%), Gaps = 10/83 (12%)
Query: 150 NGLLPGGLNSKLTRKPARSMEEMRARASTYILDEEDDAFKRKRAKLEKGDTSPEQ----- 204
+G P ++K + A+ +E ARA + ++ E+ R+++ E G+ + Q
Sbjct: 190 SGEKPNTFSNKRSEASAKKKDESVARADAHSMELEEKTHSREQSSQESGEETRRQEKPQE 249
Query: 205 -----R*KEEFQIGGKAEGKEQG 222
+ +EE Q G + E E+G
Sbjct: 250 LTDQDQSQEESQEGEEGEEGEEG 272
>MYHB_HUMAN (P35749) Myosin heavy chain, smooth muscle isoform (SMMHC)
Length = 1972
Score = 30.0 bits (66), Expect = 6.0
Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 6/85 (7%)
Query: 162 TRKPARSMEEMRARASTYI--LDEEDDAFKRKRAKLEKGDTSPEQR*KE---EFQIGGKA 216
TR ++EMR + + + L E+ + FKR +A L+K + E+ + E ++ G+A
Sbjct: 1187 TRSHEAQVQEMRQKHAQAVEELTEQLEQFKRAKANLDKNKQTLEKENADLAGELRVLGQA 1246
Query: 217 EGK-EQGAFTRE*QIEILQGASSDG 240
+ + E E Q++ LQ SDG
Sbjct: 1247 KQEVEHKKKKLEAQVQELQSKCSDG 1271
>MVIN_THEMA (Q9WXU1) Virulence factor mviN homolog
Length = 473
Score = 30.0 bits (66), Expect = 6.0
Identities = 22/78 (28%), Positives = 42/78 (53%), Gaps = 11/78 (14%)
Query: 62 RMFPSTFKSTAMAWFTTLPRGSISNFRDFSSKFLVQFSANKNQ--------PVTINDLYS 113
R+F TF + A++ TTL ++++F D S L+Q S+ Q V+ L +
Sbjct: 213 RLFFGTFMTMAVSQVTTLIDVNVASFLDPGSLSLIQLSSRLYQLPLGIFGVAVSTVALST 272
Query: 114 IRQQEG---ESLKEYVAR 128
+ + EG E+LK+++++
Sbjct: 273 LSESEGDFHENLKDFISK 290
>SYA_PSEAE (Q9I553) Alanyl-tRNA synthetase (EC 6.1.1.7)
(Alanine--tRNA ligase) (AlaRS)
Length = 874
Score = 29.6 bits (65), Expect = 7.8
Identities = 34/128 (26%), Positives = 60/128 (46%), Gaps = 23/128 (17%)
Query: 127 ARYSAASVKVEDEEPRACALAFKNGLLP-----GGLNSKLTRKPARSMEEMRARASTY-- 179
A A S+KV + R+C+ +G+LP G + ++ R+ R ++ AR + +
Sbjct: 272 ANDDAPSLKVVADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGARGTFFHK 331
Query: 180 ----ILDEEDDAF---KRKRAKLEKGDTSPEQR*KEEFQIGGKAEGKEQGAFTRE*QIEI 232
++ E DAF K+++A +E+ + E E+F A+ EQG E +
Sbjct: 332 IVAALVAEMGDAFPELKQQQAHIERVLKTEE----EQF-----AKTLEQGLKILEQDLAE 382
Query: 233 LQGASSDG 240
LQG+ G
Sbjct: 383 LQGSVIPG 390
>RCCR_ARATH (Q8LDU4) Red chlorophyll catabolite reductase,
chloroplast precursor (EC 1.-.-.-) (RCC reductase)
(AtRCCR) (Accelerated cell death protein 2)
Length = 319
Score = 29.6 bits (65), Expect = 7.8
Identities = 13/33 (39%), Positives = 20/33 (60%)
Query: 159 SKLTRKPARSMEEMRARASTYILDEEDDAFKRK 191
S LT KP+R + +R RA +++ DD +RK
Sbjct: 19 SPLTSKPSRFSKNLRPRAQFQSMEDHDDHLRRK 51
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.134 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,926,582
Number of Sequences: 164201
Number of extensions: 1111533
Number of successful extensions: 2962
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 2953
Number of HSP's gapped (non-prelim): 30
length of query: 263
length of database: 59,974,054
effective HSP length: 108
effective length of query: 155
effective length of database: 42,240,346
effective search space: 6547253630
effective search space used: 6547253630
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0192.7