BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0192.12
(60 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
GPX4_CITSI (Q06652) Probable phospholipid hydroperoxide glutathi... 101 3e-22
GPX4_MESCR (Q9LEF0) Probable phospholipid hydroperoxide glutathi... 95 3e-20
GPX1_PEA (O24296) Phospholipid hydroperoxide glutathione peroxid... 89 2e-18
GPX4_SPIOL (O23814) Probable phospholipid hydroperoxide glutathi... 89 3e-18
GPX4_LYCES (O24031) Probable phospholipid hydroperoxide glutathi... 89 3e-18
GPX2_ARATH (O04922) Probable glutathione peroxidase At2g31570 (E... 89 3e-18
GPX4_NICSY (P30708) Probable phospholipid hydroperoxide glutathi... 88 4e-18
GPX4_HELAN (O23968) Probable phospholipid hydroperoxide glutathi... 88 4e-18
GPX4_ARATH (O48646) Probable phospholipid hydroperoxide glutathi... 88 4e-18
GPX4_TOBAC (Q9FXS3) Probable phospholipid hydroperoxide glutathi... 87 6e-18
GPX1_ARATH (P52032) Phospholipid hydroperoxide glutathione perox... 87 8e-18
GPX4_GOSHI (O49069) Probable phospholipid hydroperoxide glutathi... 84 9e-17
GPX1_HELAN (O23970) Glutathione peroxidase 1 (EC 1.11.1.9) 83 2e-16
GPX3_ARATH (Q9LYB4) Probable glutathione peroxidase At3g63080 (E... 82 3e-16
GPX5_ARATH (O22850) Probable glutathione peroxidase At2g43350 (E... 75 3e-14
GPX1_CAEEL (O02621) Probable glutathione peroxidase F26E4.12 (EC... 64 6e-11
BSAA_BACSU (P52035) Glutathione peroxidase homolog bsaA 63 1e-10
GPX2_YEAST (P38143) Glutathione peroxidase 2 (EC 1.11.1.9) 62 4e-10
GPX2_CAEEL (O62327) Probable glutathione peroxidase R05H10.5 (EC... 60 1e-09
GPX4_HUMAN (P36969) Phospholipid hydroperoxide glutathione perox... 59 3e-09
>GPX4_CITSI (Q06652) Probable phospholipid hydroperoxide glutathione
peroxidase (EC 1.11.1.12) (PHGPx) (Salt-associated
protein)
Length = 167
Score = 101 bits (252), Expect = 3e-22
Identities = 48/60 (80%), Positives = 52/60 (86%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTELS LY+KYK +GLEILAF CNQF AQEPGD+EQIQEF TRFK EFP+FDK
Sbjct: 42 GLTNSNYTELSQLYDKYKNQGLEILAFPCNQFGAQEPGDNEQIQEFACTRFKAEFPIFDK 101
>GPX4_MESCR (Q9LEF0) Probable phospholipid hydroperoxide glutathione
peroxidase (EC 1.11.1.12) (PHGPx)
Length = 170
Score = 95.1 bits (235), Expect = 3e-20
Identities = 45/60 (75%), Positives = 49/60 (81%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNY EL+ LYE+YK KGLEILAF CNQF QEPGD+EQI EF TRFK EFP+FDK
Sbjct: 45 GLTNSNYPELTKLYEQYKDKGLEILAFPCNQFGNQEPGDNEQIMEFACTRFKAEFPIFDK 104
>GPX1_PEA (O24296) Phospholipid hydroperoxide glutathione
peroxidase, chloroplast precursor (EC 1.11.1.12) (PHGPx)
Length = 236
Score = 89.0 bits (219), Expect = 2e-18
Identities = 40/60 (66%), Positives = 49/60 (81%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G T+SNYTELS LYE +K KGLE+LAF CNQF QEPG +E+I++F T+FK EFP+FDK
Sbjct: 112 GLTSSNYTELSHLYENFKNKGLEVLAFPCNQFGMQEPGSNEEIKQFACTKFKAEFPIFDK 171
>GPX4_SPIOL (O23814) Probable phospholipid hydroperoxide glutathione
peroxidase (EC 1.11.1.9) (PHGPx)
Length = 171
Score = 88.6 bits (218), Expect = 3e-18
Identities = 40/60 (66%), Positives = 48/60 (79%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTE++ LYEKY+ GLEILAF CNQF QEPG +E++ EF TRFK E+P+FDK
Sbjct: 45 GLTNSNYTEMTELYEKYRELGLEILAFPCNQFGNQEPGSNEEVLEFACTRFKAEYPIFDK 104
>GPX4_LYCES (O24031) Probable phospholipid hydroperoxide glutathione
peroxidase (EC 1.11.1.12) (PHGPx)
Length = 169
Score = 88.6 bits (218), Expect = 3e-18
Identities = 40/60 (66%), Positives = 49/60 (81%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYT+++ LY+KYK +GLEILAF CNQF QEPG+ E IQ+ V TRFK E+P+FDK
Sbjct: 44 GLTNSNYTDMTELYKKYKDQGLEILAFPCNQFGGQEPGNIEDIQQMVCTRFKAEYPIFDK 103
>GPX2_ARATH (O04922) Probable glutathione peroxidase At2g31570 (EC
1.11.1.9)
Length = 169
Score = 88.6 bits (218), Expect = 3e-18
Identities = 41/60 (68%), Positives = 50/60 (83%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G T++NY EL+ LYEKYK +GLEILAF CNQF QEPG++E+IQ+ V TRFK EFP+FDK
Sbjct: 42 GLTDANYKELNVLYEKYKEQGLEILAFPCNQFLGQEPGNNEEIQQTVCTRFKAEFPIFDK 101
>GPX4_NICSY (P30708) Probable phospholipid hydroperoxide glutathione
peroxidase (EC 1.11.1.12) (PHGPx) (6P229)
Length = 169
Score = 88.2 bits (217), Expect = 4e-18
Identities = 40/60 (66%), Positives = 48/60 (79%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYT+L+ +Y+KYK +GLEILAF CNQF QEPG E+IQ V TRFK E+P+FDK
Sbjct: 44 GLTNSNYTDLTEIYKKYKDQGLEILAFPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDK 103
>GPX4_HELAN (O23968) Probable phospholipid hydroperoxide glutathione
peroxidase (EC 1.11.1.12) (PHGPx) (Glutathione
peroxidase 2)
Length = 180
Score = 88.2 bits (217), Expect = 4e-18
Identities = 41/60 (68%), Positives = 47/60 (78%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GFTNSNY EL+ LY+KYK +G EILAF CNQF QEPG +E+IQ F TRFK E+PVF K
Sbjct: 55 GFTNSNYPELTTLYQKYKDQGFEILAFPCNQFGGQEPGSNEEIQVFACTRFKAEYPVFSK 114
>GPX4_ARATH (O48646) Probable phospholipid hydroperoxide glutathione
peroxidase, mitochondrial precursor (EC 1.11.1.12)
(PHGPx) (AtGPX1)
Length = 232
Score = 88.2 bits (217), Expect = 4e-18
Identities = 41/60 (68%), Positives = 47/60 (78%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTEL+ LYEKYK G EILAF CNQF QEPG +E+I +F TRFK E+P+FDK
Sbjct: 106 GLTNSNYTELAQLYEKYKGHGFEILAFPCNQFGNQEPGTNEEIVQFACTRFKAEYPIFDK 165
>GPX4_TOBAC (Q9FXS3) Probable phospholipid hydroperoxide glutathione
peroxidase (EC 1.11.1.9) (PHGPx) (Nt-SubC08)
Length = 169
Score = 87.4 bits (215), Expect = 6e-18
Identities = 39/60 (65%), Positives = 48/60 (80%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYT+++ +Y+KYK +GLEILAF CNQF QEPG E+IQ V TRFK E+P+FDK
Sbjct: 44 GLTNSNYTDMTEIYKKYKDQGLEILAFPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDK 103
>GPX1_ARATH (P52032) Phospholipid hydroperoxide glutathione
peroxidase, chloroplast precursor (EC 1.11.1.12) (PHGPx)
Length = 236
Score = 87.0 bits (214), Expect = 8e-18
Identities = 40/60 (66%), Positives = 48/60 (79%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G T+SNY+ELS LYEKYK +G EILAF CNQF QEPG + +I++F TRFK EFP+FDK
Sbjct: 112 GLTSSNYSELSHLYEKYKTQGFEILAFPCNQFGFQEPGSNSEIKQFACTRFKAEFPIFDK 171
>GPX4_GOSHI (O49069) Probable phospholipid hydroperoxide glutathione
peroxidase (EC 1.11.1.12) (PHGPx)
Length = 170
Score = 83.6 bits (205), Expect = 9e-17
Identities = 40/61 (65%), Positives = 47/61 (76%), Gaps = 1/61 (1%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGD-SEQIQEFV*TRFKIEFPVFD 59
G TNSNYT+L+ +Y+KYK +GLEILAF CNQF QEPG E IQ V TRFK E+P+FD
Sbjct: 44 GLTNSNYTDLTEIYKKYKDQGLEILAFPCNQFGGQEPGSIEESIQNMVCTRFKAEYPIFD 103
Query: 60 K 60
K
Sbjct: 104 K 104
>GPX1_HELAN (O23970) Glutathione peroxidase 1 (EC 1.11.1.9)
Length = 167
Score = 82.8 bits (203), Expect = 2e-16
Identities = 38/60 (63%), Positives = 47/60 (78%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TN++Y EL+ +Y KYK KG EILAF CNQF QEPG +E+I +FV T+FK EFP+FDK
Sbjct: 42 GLTNNSYDELNQIYLKYKEKGFEILAFPCNQFGQQEPGTNEEIVDFVCTKFKSEFPIFDK 101
>GPX3_ARATH (Q9LYB4) Probable glutathione peroxidase At3g63080 (EC
1.11.1.9)
Length = 173
Score = 82.0 bits (201), Expect = 3e-16
Identities = 37/60 (61%), Positives = 45/60 (74%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GFT SNYT+L+ LY KYK +G +LAF CNQF +QEPG SE+ +F TRFK E+PVF K
Sbjct: 47 GFTESNYTQLTELYRKYKDQGFVVLAFPCNQFLSQEPGTSEEAHQFACTRFKAEYPVFQK 106
>GPX5_ARATH (O22850) Probable glutathione peroxidase At2g43350 (EC
1.11.1.9)
Length = 206
Score = 75.1 bits (183), Expect = 3e-14
Identities = 35/60 (58%), Positives = 44/60 (73%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G T+ NY E++ LY KYK +G EILAF CNQF +QEPG + +I+E V FK EFP+FDK
Sbjct: 81 GLTHGNYKEMNILYAKYKTQGFEILAFPCNQFGSQEPGSNMEIKETVCNIFKAEFPIFDK 140
>GPX1_CAEEL (O02621) Probable glutathione peroxidase F26E4.12 (EC
1.11.1.9)
Length = 163
Score = 64.3 bits (155), Expect = 6e-11
Identities = 32/60 (53%), Positives = 37/60 (61%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TN NYT+L L + YK GLE+LAF CNQF QEP IQ FV +FK E +F K
Sbjct: 37 GLTNKNYTQLKELLDVYKKDGLEVLAFPCNQFAGQEPSCEIDIQAFVADKFKFEPTLFQK 96
>BSAA_BACSU (P52035) Glutathione peroxidase homolog bsaA
Length = 160
Score = 63.2 bits (152), Expect = 1e-10
Identities = 31/60 (51%), Positives = 39/60 (64%), Gaps = 1/60 (1%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GFT S +L LY+ Y+ +GLEIL F CNQF QEPG+ IQEF T + + FP+F K
Sbjct: 36 GFT-SQLKQLQELYDTYQQEGLEILGFPCNQFMNQEPGEEADIQEFCETNYGVTFPMFSK 94
>GPX2_YEAST (P38143) Glutathione peroxidase 2 (EC 1.11.1.9)
Length = 162
Score = 61.6 bits (148), Expect = 4e-10
Identities = 31/60 (51%), Positives = 36/60 (59%), Gaps = 1/60 (1%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GFT Y EL LY+KY+ KG IL F CNQF QEPG EQI EF + + FP+ K
Sbjct: 38 GFT-PQYKELEELYKKYQDKGFVILGFPCNQFGKQEPGSDEQITEFCQLNYGVTFPIMKK 96
>GPX2_CAEEL (O62327) Probable glutathione peroxidase R05H10.5 (EC
1.11.1.9)
Length = 163
Score = 59.7 bits (143), Expect = 1e-09
Identities = 30/60 (50%), Positives = 35/60 (58%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNY + L + YK GLE+LAF CNQF QEP I FV +FK E +F K
Sbjct: 37 GLTNSNYNQFKELLDVYKKDGLEVLAFPCNQFGGQEPSCEIDIAAFVADKFKFEPTLFQK 96
>GPX4_HUMAN (P36969) Phospholipid hydroperoxide glutathione
peroxidase, mitochondrial precursor (EC 1.11.1.12)
(PHGPx) (GPX-4)
Length = 197
Score = 58.5 bits (140), Expect = 3e-09
Identities = 29/60 (48%), Positives = 39/60 (64%), Gaps = 1/60 (1%)
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G T NYT+L L+ +Y GL ILAF CNQF QEPG +E+I+EF + ++F +F K
Sbjct: 74 GKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSK 132
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.337 0.151 0.469
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,306,876
Number of Sequences: 164201
Number of extensions: 169282
Number of successful extensions: 471
Number of sequences better than 10.0: 73
Number of HSP's better than 10.0 without gapping: 69
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 394
Number of HSP's gapped (non-prelim): 74
length of query: 60
length of database: 59,974,054
effective HSP length: 36
effective length of query: 24
effective length of database: 54,062,818
effective search space: 1297507632
effective search space used: 1297507632
T: 11
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0192.12