BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0185.2
(122 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TRA1_MAIZE (P08770) Putative AC transposase (ORFA) 106 8e-24
TRA9_MAIZE (P03010) Putative AC9 transposase 105 2e-23
HOBO_DROME (P12258) Transposable element Hobo transposase 43 1e-04
ZBE1_HUMAN (O96006) Zinc finger BED domain containing protein 1 ... 35 0.039
YLZ5_CAEEL (P34418) Hypothetical protein F42H10.5 in chromosome III 33 0.11
PSB3_DROME (Q9XYN7) Proteasome subunit beta type 3 (EC 3.4.25.1)... 29 1.6
SYM_PYRHO (O58721) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 29 2.1
TOM1_YEAST (Q03280) E3 ubiquitin protein ligase TOM1 (EC 6.3.2.-... 28 2.8
PUR2_SALTY (P26977) Phosphoribosylamine--glycine ligase (EC 6.3.... 28 4.8
PUR2_SALTI (Q8Z334) Phosphoribosylamine--glycine ligase (EC 6.3.... 28 4.8
MTHS_YEAST (P53128) Methylenetetrahydrofolate reductase 2 (EC 1.... 28 4.8
ASSY_METMP (P61527) Argininosuccinate synthase (EC 6.3.4.5) (Cit... 28 4.8
ACCD_MYCTU (P63405) Putative acetyl-coenzyme A carboxylase carbo... 27 6.2
ACCD_MYCBO (P63406) Putative acetyl-coenzyme A carboxylase carbo... 27 6.2
SYM_PYRAB (Q9V011) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 27 8.1
PROA_LEPIN (P94872) Gamma-glutamyl phosphate reductase (GPR) (EC... 27 8.1
PROA_LEPIC (Q72NQ9) Gamma-glutamyl phosphate reductase (GPR) (EC... 27 8.1
CYP2_CAEEL (P52010) Peptidyl-prolyl cis-trans isomerase 2 (EC 5.... 27 8.1
ATPG_BOVIN (P05631) ATP synthase gamma chain, mitochondrial prec... 27 8.1
>TRA1_MAIZE (P08770) Putative AC transposase (ORFA)
Length = 806
Score = 106 bits (265), Expect = 8e-24
Identities = 55/105 (52%), Positives = 75/105 (71%), Gaps = 6/105 (5%)
Query: 4 NELTKYLEDGL-EERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGR 62
NEL KY+ + L + G DIL+WW+ + YPIL IAR++LAI +STVASES FSAGGR
Sbjct: 667 NELDKYMSEPLLKHSGQFDILSWWRGRVAEYPILTQIARDVLAIQVSTVASESAFSAGGR 726
Query: 63 VVDPYRSSLTPKTLEALICTQDWI----KG-KYSKSLLSNEEVME 102
VVDPYR+ L + +EALICT+DW+ KG Y +++ + EV++
Sbjct: 727 VVDPYRNRLGSEIVEALICTKDWVAASRKGATYFPTMIGDLEVLD 771
>TRA9_MAIZE (P03010) Putative AC9 transposase
Length = 839
Score = 105 bits (262), Expect = 2e-23
Identities = 50/84 (59%), Positives = 64/84 (75%), Gaps = 1/84 (1%)
Query: 4 NELTKYLEDGL-EERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGR 62
NEL KY+ + L + G DIL+WW+ + YPIL IAR++LAI +STVASES FSAGGR
Sbjct: 637 NELDKYMSEPLLKHSGQFDILSWWRGRVAEYPILTQIARDVLAIQVSTVASESAFSAGGR 696
Query: 63 VVDPYRSSLTPKTLEALICTQDWI 86
VVDPYR+ L + +EALICT+DW+
Sbjct: 697 VVDPYRNRLGSEIVEALICTKDWV 720
>HOBO_DROME (P12258) Transposable element Hobo transposase
Length = 644
Score = 42.7 bits (99), Expect = 1e-04
Identities = 17/54 (31%), Positives = 33/54 (60%)
Query: 4 NELTKYLEDGLEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTF 57
+E+ +Y+ + + +++ WWK NA+ YP L+ +A +LL+IP S+ + F
Sbjct: 590 DEIERYIRQRVPLSQNFEVIEWWKNNANLYPQLSKLALKLLSIPASSAELKECF 643
>ZBE1_HUMAN (O96006) Zinc finger BED domain containing protein 1
(dREF homolog) (Putative Ac-like transposable element)
Length = 694
Score = 34.7 bits (78), Expect = 0.039
Identities = 15/53 (28%), Positives = 25/53 (46%)
Query: 21 DILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYRSSLTP 73
D L WW + +P+L + ++ + + VA E F + VV R+ L P
Sbjct: 586 DPLKWWSDRLALFPLLPKVLQKYWCVTATRVAPERLFGSAANVVSAKRNRLAP 638
>YLZ5_CAEEL (P34418) Hypothetical protein F42H10.5 in chromosome III
Length = 810
Score = 33.1 bits (74), Expect = 0.11
Identities = 16/36 (44%), Positives = 24/36 (66%)
Query: 23 LNWWKLNASRYPILASIARELLAIPISTVASESTFS 58
L++WK +SR L+ +A ELL+IPI T+ +E S
Sbjct: 734 LSYWKSCSSRCSELSDLATELLSIPIFTLTAERVLS 769
>PSB3_DROME (Q9XYN7) Proteasome subunit beta type 3 (EC 3.4.25.1)
(20S proteasome subunit beta-3)
Length = 205
Score = 29.3 bits (64), Expect = 1.6
Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 2/48 (4%)
Query: 41 RELLAIPISTVASESTFSA--GGRVVDPYRSSLTPKTLEALICTQDWI 86
RE+ P S + S + G ++P + L PKT+E IC D I
Sbjct: 80 REMCPKPFSAMMSSFLYEHRFGPYFIEPVVAGLDPKTMEPFICNMDLI 127
>SYM_PYRHO (O58721) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 723
Score = 28.9 bits (63), Expect = 2.1
Identities = 12/23 (52%), Positives = 16/23 (69%)
Query: 3 KNELTKYLEDGLEERGSLDILNW 25
KN + K++E+GLEER LNW
Sbjct: 213 KNMVLKWIEEGLEERAITRDLNW 235
>TOM1_YEAST (Q03280) E3 ubiquitin protein ligase TOM1 (EC 6.3.2.-)
(Temperature dependent-organization in mitotic nucleus
protein 1) (Suppressor of snRNA protein 2)
Length = 3268
Score = 28.5 bits (62), Expect = 2.8
Identities = 22/89 (24%), Positives = 44/89 (48%), Gaps = 4/89 (4%)
Query: 27 KLNASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYRSSLTPKTLEALICTQDWI 86
K A R ++ +AR ++ ++TV + T ++ DP+ + + +EA+I I
Sbjct: 1716 KHEAKRREVIGMLARSVIIGFLATVQDDRTTKTDVKLADPHMNFIRKFAIEAIIKA---I 1772
Query: 87 KGKYSKSLLSNEEVMELEKYEQGIITNYV 115
+ S S L ++L+ + + IIT+ V
Sbjct: 1773 RNATSSSKLLESNHLKLDMWFR-IITSMV 1800
>PUR2_SALTY (P26977) Phosphoribosylamine--glycine ligase (EC
6.3.4.13) (GARS) (Glycinamide ribonucleotide synthetase)
(Phosphoribosylglycinamide synthetase)
Length = 429
Score = 27.7 bits (60), Expect = 4.8
Identities = 17/54 (31%), Positives = 26/54 (47%), Gaps = 4/54 (7%)
Query: 15 EERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYR 68
+ER SL ++ + A YP S E+ +P+ VA F AG ++ D R
Sbjct: 327 DERASLGVV----IAAGGYPGSYSTGDEIHGLPLEEVADGKVFHAGTKLADDDR 376
>PUR2_SALTI (Q8Z334) Phosphoribosylamine--glycine ligase (EC
6.3.4.13) (GARS) (Glycinamide ribonucleotide synthetase)
(Phosphoribosylglycinamide synthetase)
Length = 429
Score = 27.7 bits (60), Expect = 4.8
Identities = 17/54 (31%), Positives = 26/54 (47%), Gaps = 4/54 (7%)
Query: 15 EERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYR 68
+ER SL ++ + A YP S E+ +P+ VA F AG ++ D R
Sbjct: 327 DERASLGVV----IAAGGYPGSYSTGDEIHGLPLEEVADGKVFHAGTKLADDDR 376
>MTHS_YEAST (P53128) Methylenetetrahydrofolate reductase 2 (EC
1.5.1.20)
Length = 599
Score = 27.7 bits (60), Expect = 4.8
Identities = 22/78 (28%), Positives = 36/78 (45%), Gaps = 7/78 (8%)
Query: 14 LEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYRSSLTP 73
L + G + L+ + +N + P++ I L +P +ES F+A V P+R SL P
Sbjct: 269 LLDSGYVSHLHIYTMNLEKAPLM--ILERLNILP-----TESEFNAHPLAVLPWRKSLNP 321
Query: 74 KTLEALICTQDWIKGKYS 91
K + W + YS
Sbjct: 322 KRKNEEVRPIFWKRRPYS 339
>ASSY_METMP (P61527) Argininosuccinate synthase (EC 6.3.4.5)
(Citrulline--aspartate ligase)
Length = 397
Score = 27.7 bits (60), Expect = 4.8
Identities = 21/56 (37%), Positives = 27/56 (47%), Gaps = 2/56 (3%)
Query: 6 LTKYLEDGLEERGSLDILNWWKLNA--SRYPILASIARELLAIPISTVASESTFSA 59
L Y D EE I K NA YP+ ++AR L+AI I+ +A E SA
Sbjct: 58 LKHYTIDAKEEFAKDYIFRAIKANALYEGYPLSTALARPLIAIKIAELAQEVGASA 113
>ACCD_MYCTU (P63405) Putative acetyl-coenzyme A carboxylase
carboxyl transferase subunit beta (EC 6.4.1.2) (ACCASE
beta chain)
Length = 495
Score = 27.3 bits (59), Expect = 6.2
Identities = 20/57 (35%), Positives = 25/57 (43%), Gaps = 3/57 (5%)
Query: 7 TKYLEDGLEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRV 63
T L + +RGS W P+ S AREL A +T A ES + GRV
Sbjct: 6 TDQLRHAVLDRGSFVS---WDSEPLAVPVADSYARELAAARAATGADESVQTGEGRV 59
>ACCD_MYCBO (P63406) Putative acetyl-coenzyme A carboxylase
carboxyl transferase subunit beta (EC 6.4.1.2) (ACCASE
beta chain)
Length = 495
Score = 27.3 bits (59), Expect = 6.2
Identities = 20/57 (35%), Positives = 25/57 (43%), Gaps = 3/57 (5%)
Query: 7 TKYLEDGLEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRV 63
T L + +RGS W P+ S AREL A +T A ES + GRV
Sbjct: 6 TDQLRHAVLDRGSFVS---WDSEPLAVPVADSYARELAAARAATGADESVQTGEGRV 59
>SYM_PYRAB (Q9V011) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 722
Score = 26.9 bits (58), Expect = 8.1
Identities = 11/23 (47%), Positives = 15/23 (64%)
Query: 3 KNELTKYLEDGLEERGSLDILNW 25
KN + ++E+GLEER LNW
Sbjct: 213 KNMVLSWIEEGLEERAITRDLNW 235
>PROA_LEPIN (P94872) Gamma-glutamyl phosphate reductase (GPR) (EC
1.2.1.41) (Glutamate-5-semialdehyde dehydrogenase)
(Glutamyl-gamma-semialdehyde dehydrogenase) (GSA
dehydrogenase)
Length = 416
Score = 26.9 bits (58), Expect = 8.1
Identities = 17/44 (38%), Positives = 26/44 (58%), Gaps = 2/44 (4%)
Query: 4 NELTKYLEDGLEERGSLDILNWWKLNASRYPILASIARELLAIP 47
NEL L+DG E++ S +++ LN R +AS RE+ A+P
Sbjct: 51 NELD--LKDGKEKKLSSALMDRLLLNEKRIFSMASAVREIAALP 92
>PROA_LEPIC (Q72NQ9) Gamma-glutamyl phosphate reductase (GPR) (EC
1.2.1.41) (Glutamate-5-semialdehyde dehydrogenase)
(Glutamyl-gamma-semialdehyde dehydrogenase) (GSA
dehydrogenase)
Length = 416
Score = 26.9 bits (58), Expect = 8.1
Identities = 17/44 (38%), Positives = 26/44 (58%), Gaps = 2/44 (4%)
Query: 4 NELTKYLEDGLEERGSLDILNWWKLNASRYPILASIARELLAIP 47
NEL L+DG E++ S +++ LN R +AS RE+ A+P
Sbjct: 51 NELD--LKDGKEKKLSSALMDRLLLNEKRIFSMASAVREIAALP 92
>CYP2_CAEEL (P52010) Peptidyl-prolyl cis-trans isomerase 2 (EC
5.2.1.8) (PPIase) (Rotamase) (Cyclophilin-2)
Length = 172
Score = 26.9 bits (58), Expect = 8.1
Identities = 15/37 (40%), Positives = 20/37 (53%), Gaps = 2/37 (5%)
Query: 60 GGRVVDPYRSSLTPKTLE--ALICTQDWIKGKYSKSL 94
GGR+V + + PKT E +CT + KGK K L
Sbjct: 17 GGRIVMELYNDIVPKTAENFRALCTGEKGKGKSGKKL 53
>ATPG_BOVIN (P05631) ATP synthase gamma chain, mitochondrial
precursor (EC 3.6.3.14)
Length = 298
Score = 26.9 bits (58), Expect = 8.1
Identities = 17/70 (24%), Positives = 32/70 (45%)
Query: 46 IPISTVASESTFSAGGRVVDPYRSSLTPKTLEALICTQDWIKGKYSKSLLSNEEVMELEK 105
I + + S F G + + +RS ++ KT E I + D I S S+ + + L
Sbjct: 169 IALELLNSGYEFDEGSIIFNRFRSVISYKTEEKPIFSLDTISSAESMSIYDDIDADVLRN 228
Query: 106 YEQGIITNYV 115
Y++ + N +
Sbjct: 229 YQEYSLANII 238
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.139 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,502,668
Number of Sequences: 164201
Number of extensions: 457486
Number of successful extensions: 1243
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1233
Number of HSP's gapped (non-prelim): 20
length of query: 122
length of database: 59,974,054
effective HSP length: 98
effective length of query: 24
effective length of database: 43,882,356
effective search space: 1053176544
effective search space used: 1053176544
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0185.2