BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0180a.6
         (731 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

MACF_MOUSE (Q9QXZ0) Microtubule-actin crosslinking factor 1 (Act...    35  0.54
HS82_YEAST (P02829) ATP-dependent molecular chaperone HSP82 (Hea...    35  0.70
HS83_YEAST (P15108) ATP-dependent molecular chaperone HSC82 (Hea...    35  0.92
EYA3_MOUSE (P97480) Eyes absent homolog 3 (EC 3.1.3.48)                35  0.92
EYA3_HUMAN (Q99504) Eyes absent homolog 3 (EC 3.1.3.48)                34  1.2
EYA3_CHICK (Q9YH99) Eyes absent homolog 3 (EC 3.1.3.48) (Fragment)     34  1.2
YEN1_YEAST (P40028) Hypothetical protein YER41W                        34  1.6
Y162_METJA (Q57626) Hypothetical protein MJ0162                        33  3.5
YM24_YEAST (P40218) Hypothetical 25.9 kDa protein in TIF34-SWP1 ...    32  6.0
U520_HUMAN (O75643) U5 small nuclear ribonucleoprotein 200 kDa h...    32  7.8
STE7_YEAST (P06784) Serine/threonine-protein kinase STE7 (EC 2.7...    32  7.8

>MACF_MOUSE (Q9QXZ0) Microtubule-actin crosslinking factor 1 (Actin
           cross-linking family 7)
          Length = 5327

 Score = 35.4 bits (80), Expect = 0.54
 Identities = 27/92 (29%), Positives = 44/92 (47%), Gaps = 3/92 (3%)

Query: 15  ENFLRNHQNSLKSLFLRNSSSSKNKTDTTADDSAVNSPKPIPLLSPLANSVVVRSSKILG 74
           E+FLRN Q+S+K  +  + S+S ++ +    DS     + I   S +A S+V RS  I+ 
Sbjct: 799 ESFLRNLQDSIKRKYTADRSTSLSRLEDLLQDSMDEKEQLIQSKSSVA-SLVGRSKTIVQ 857

Query: 75  ISTEELQHAFDSELPLGVKELLTYARNLLEFC 106
           +      H   S   L VK +  Y +  +  C
Sbjct: 858 LKPRNPDHVLKS--TLSVKAICDYRQIEITIC 887


>HS82_YEAST (P02829) ATP-dependent molecular chaperone HSP82 (Heat
           shock protein Hsp90 heat inducible isoform) (82 kDa heat
           shock protein)
          Length = 709

 Score = 35.0 bits (79), Expect = 0.70
 Identities = 26/95 (27%), Positives = 41/95 (42%), Gaps = 2/95 (2%)

Query: 299 HEKPVRYDLGTDLKQVIKPDLTGPLGARLFDKAVMYKSTSVAEPVYFEFPEFKANVRRDY 358
           H+K + Y  G  LK V K      L A+ F+  V++ +  + E  + +  EF+     D 
Sbjct: 467 HQKNIYYITGESLKAVEKSPFLDALKAKNFE--VLFLTDPIDEYAFTQLKEFEGKTLVDI 524

Query: 359 WLDISLEILRVHKFIRKYYLKETQKAEVLARAILG 393
             D  LE     K  R+  +KE +      + ILG
Sbjct: 525 TKDFELEETDEEKAEREKEIKEYEPLTKALKEILG 559


>HS83_YEAST (P15108) ATP-dependent molecular chaperone HSC82 (Heat
           shock protein Hsp90 constitutive isoform) (82 kDa heat
           shock cognate protein)
          Length = 704

 Score = 34.7 bits (78), Expect = 0.92
 Identities = 26/95 (27%), Positives = 41/95 (42%), Gaps = 2/95 (2%)

Query: 299 HEKPVRYDLGTDLKQVIKPDLTGPLGARLFDKAVMYKSTSVAEPVYFEFPEFKANVRRDY 358
           H+K + Y  G  LK V K      L A+ F+  V++ +  + E  + +  EF+     D 
Sbjct: 462 HQKNIYYITGESLKAVEKSPFLDALKAKNFE--VLFLTDPIDEYAFTQLKEFEGKTLVDI 519

Query: 359 WLDISLEILRVHKFIRKYYLKETQKAEVLARAILG 393
             D  LE     K  R+  +KE +      + ILG
Sbjct: 520 TKDFELEETDEEKAEREKEIKEYEPLTKALKDILG 554


>EYA3_MOUSE (P97480) Eyes absent homolog 3 (EC 3.1.3.48)
          Length = 510

 Score = 34.7 bits (78), Expect = 0.92
 Identities = 33/117 (28%), Positives = 55/117 (46%), Gaps = 13/117 (11%)

Query: 395 YRYRAVREAFKYFSSHYKTLLSFNLAEALPRGDMILETLSNS-----LTNLTVASSKRSS 449
           +RYR VRE +    S+   LLS    EAL R    +E L++S     L +L +  S+++ 
Sbjct: 354 FRYRKVREIYDKHKSNVGGLLSPQRKEALQRLRAEIEVLTDSWLGTALKSLLLIQSRKNC 413

Query: 450 PRNVDTKKQLVVSPASAVALFRLGFKSDKFADIYEETTVVGDIRVGEINLLEMAVKK 506
              + T  QLV + A  V L+ LG       +I+    +    ++G+ +  E  V +
Sbjct: 414 ANVLITTTQLVPALAK-VLLYGLG-------EIFPIENIYSATKIGKESCFERIVSR 462


>EYA3_HUMAN (Q99504) Eyes absent homolog 3 (EC 3.1.3.48)
          Length = 573

 Score = 34.3 bits (77), Expect = 1.2
 Identities = 33/117 (28%), Positives = 55/117 (46%), Gaps = 13/117 (11%)

Query: 395 YRYRAVREAFKYFSSHYKTLLSFNLAEALPRGDMILETLSNS-----LTNLTVASSKRSS 449
           +RYR VRE +    S+   LLS    EAL R    +E L++S     L +L +  S+++ 
Sbjct: 417 FRYRKVREIYDKHKSNVGGLLSPQRKEALQRLRAEIEVLTDSWLGTALKSLLLIQSRKNC 476

Query: 450 PRNVDTKKQLVVSPASAVALFRLGFKSDKFADIYEETTVVGDIRVGEINLLEMAVKK 506
              + T  QLV + A  V L+ LG       +I+    +    ++G+ +  E  V +
Sbjct: 477 VNVLITTTQLVPALAK-VLLYGLG-------EIFPIENIYSATKIGKESCFERIVSR 525


>EYA3_CHICK (Q9YH99) Eyes absent homolog 3 (EC 3.1.3.48) (Fragment)
          Length = 119

 Score = 34.3 bits (77), Expect = 1.2
 Identities = 31/117 (26%), Positives = 56/117 (47%), Gaps = 13/117 (11%)

Query: 395 YRYRAVREAFKYFSSHYKTLLSFNLAEALPRGDMILETLSNS-----LTNLTVASSKRSS 449
           +RYR VRE +  + ++   LLS    EAL R    +E L++S     L +L +  S+++ 
Sbjct: 5   FRYRRVREIYDKYKTNVGGLLSPQKREALQRLRTDIEVLTDSWLETALKSLLLIQSRKNC 64

Query: 450 PRNVDTKKQLVVSPASAVALFRLGFKSDKFADIYEETTVVGDIRVGEINLLEMAVKK 506
              + T  QLV + A  V L+ LG       +++    +    ++G+ +  E  V +
Sbjct: 65  VNILITTTQLVPALAK-VLLYGLG-------EVFPIENIYSATKIGKESCFERIVSR 113


>YEN1_YEAST (P40028) Hypothetical protein YER41W
          Length = 759

 Score = 33.9 bits (76), Expect = 1.6
 Identities = 40/176 (22%), Positives = 75/176 (41%), Gaps = 20/176 (11%)

Query: 14  WENFLRNHQNSLKSLFLRNSSSSKNKTDTTADDSAVNSPKPIPL---LSPLANSVVVRSS 70
           +  FL N   S +S   R++S S++K+ T      +  P  + L   L P ++ +V++  
Sbjct: 487 YTTFLPNIPISSQSPLKRSNSPSRSKSPTRRQMDIMEHPNSLWLPKYLIPQSHPLVIQYY 546

Query: 71  KILGISTEELQHAFDSELPLGVKELLTYARNLLEFCS-----YKALHKLSKSSDYLADKD 125
           +    + + +Q   + +     K  L    NL EF        K++ K+ +S   + +  
Sbjct: 547 E----TQQLIQKEKEKKGKKSNKSRLPQKNNLDEFLRKHTSPIKSIGKVGESRKEILEPV 602

Query: 126 FRRLTYDMMLAWETPSVHTDETPPPS---SKDEHGGDDDEASLFYSSSTNMAVQVD 178
            +RL  D            +E P P+   + DEH  +DD++ +F    TN    +D
Sbjct: 603 RKRLFVDT-----DEDTSLEEIPAPTRLTTVDEHSDNDDDSLIFVDEITNSQSVLD 653


>Y162_METJA (Q57626) Hypothetical protein MJ0162
          Length = 421

 Score = 32.7 bits (73), Expect = 3.5
 Identities = 52/235 (22%), Positives = 92/235 (39%), Gaps = 30/235 (12%)

Query: 78  EELQHAFDSELPLGVKELLTYARNLLEFCSYKALHKLSKSSDYLADKDFRRLTYDMMLAW 137
           E++QHA ++   L   E      N+ +F  Y A H L  +S YL + D +++ Y   +  
Sbjct: 99  EDIQHAMENIECLNYYEERQITENI-KFKFYNAGHILGSASIYL-EVDGKKILYTGDI-- 154

Query: 138 ETPSVHTDETPPPSSKDE---------HGGDDDEASLFYSSSTNMAVQVDD------QKT 182
               V     P  +  DE         +G   D      +    +  ++ +      +  
Sbjct: 155 -NEGVSRTLLPADTDIDEIDVLIIESTYGSPLDIKPARKTLERQLIEEISETIENGGKVI 213

Query: 183 VGLEAFSRIAPVCAPIADVITVHNLFDALTSSSGRRLHFL-VYDKYIRFLDKVIKNSKNV 241
           + + A  R   +   I + I    L D    + G  +H   VY  YI +L+  IKN    
Sbjct: 214 IPVFAIGRAQEILLIINNYIRSGKLRDVPIYTDGSLIHATAVYMSYINWLNPKIKNMVEN 273

Query: 242 LASSVGSLQLAEEE--------IVLDVDGTIPTQPVLQHIGISAWP-GRLTLTNY 287
             +  G ++ A+E         I++   G +   PVL+++ +   P  +L LT Y
Sbjct: 274 RINPFGEIKKADESLVFNKEPCIIVSTSGMVQGGPVLKYLKLLKDPKNKLILTGY 328


>YM24_YEAST (P40218) Hypothetical 25.9 kDa protein in TIF34-SWP1
           intergenic region
          Length = 223

 Score = 32.0 bits (71), Expect = 6.0
 Identities = 24/91 (26%), Positives = 41/91 (44%), Gaps = 14/91 (15%)

Query: 116 KSSDY-LADKDFRRLTYDMMLAWETPSVHTDETPPPSSK----------DEHGGDDDEAS 164
           K++ Y L  ++  + T D+ +  ETP     E P P+ +          DEH   +DE S
Sbjct: 24  KNATYVLVAEELHKKTIDLNMGTETPLTENHENPIPAKEFKHQQKLEPIDEHDDGEDELS 83

Query: 165 LFYSSSTNMA---VQVDDQKTVGLEAFSRIA 192
           + + S T  +    + + QK +   AF+  A
Sbjct: 84  IKFKSMTKSSGPITEAEVQKLLLSYAFTSAA 114


>U520_HUMAN (O75643) U5 small nuclear ribonucleoprotein 200 kDa
           helicase (EC 3.6.1.-) (U5 snRNP-specific 200 kDa
           protein) (U5-200KD) (Activating signal cointegrator 1
           complex subunit 3-like 1) (Fragment)
          Length = 1701

 Score = 31.6 bits (70), Expect = 7.8
 Identities = 35/116 (30%), Positives = 58/116 (49%), Gaps = 14/116 (12%)

Query: 442 VASSKRSSPRNVDTKKQLVVSPASAVALFRLGFKSDKFADIYEETTVVGDIRVGEINLLE 501
           +AS    +   V T  QL+    S + LFR+   S +F +I    TV  + ++    LLE
Sbjct: 552 IASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNI----TVREEEKLELQKLLE 607

Query: 502 ---MAVKKSLRDTGKA--EAAQATVDQVKVEGIDTNVAVMKELLFPVIESANRLER 552
              + VK+S+ +         QA + Q+K+EG     A+M ++++ V +SA RL R
Sbjct: 608 RVPIPVKESIEEPSAKINVLLQAFISQLKLEGF----ALMADMVY-VTQSAGRLMR 658


>STE7_YEAST (P06784) Serine/threonine-protein kinase STE7 (EC
           2.7.1.37)
          Length = 515

 Score = 31.6 bits (70), Expect = 7.8
 Identities = 18/55 (32%), Positives = 26/55 (46%), Gaps = 4/55 (7%)

Query: 16  NFLRNHQNSLKSLFLRNSSSSKNKTDTTADDSAVNSPKPIPLLSPLANSVVVRSS 70
           N ++N+ N    LFLR     K   D   DD A++ P  + +  P    V+V SS
Sbjct: 51  NAIQNNSN----LFLRRGIKKKLTLDAFGDDQAISKPNTVVIQQPQNEPVLVLSS 101


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.320    0.135    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,113,449
Number of Sequences: 164201
Number of extensions: 3312500
Number of successful extensions: 9171
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 9168
Number of HSP's gapped (non-prelim): 14
length of query: 731
length of database: 59,974,054
effective HSP length: 118
effective length of query: 613
effective length of database: 40,598,336
effective search space: 24886779968
effective search space used: 24886779968
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 70 (31.6 bits)