BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0176a.2
         (342 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YCT4_YEAST (P25625) Hypothetical 42.5 kDa protein in TSM1-ARE1 i...   118  2e-26
YEJM_ECOLI (P33922) Hypothetical protein yejM                          32  3.0
RIR2_YEAST (P09938) Ribonucleoside-diphosphate reductase small c...    32  3.0
HLY3_BACSU (P54175) Hemolysin III homolog                              32  3.0
BOSS_DROVI (Q24738) Bride of sevenless protein precursor               31  3.9
YNI7_YEAST (P48231) Hypothetical 132.5 kDa protein in TOP2-MKT1 ...    31  5.2
YCX1_ODOSI (P49827) Hypothetical 41.6 kDa protein in psaA-ycf32....    30  6.7
TME8_MOUSE (Q9ESN3) Transmembrane protein 8 precursor (M83 protein)    30  6.7
GLTS_HAEIN (P45240) Sodium/glutamate symport carrier protein (Gl...    30  6.7
SCAA_XENLA (P51167) Amiloride-sensitive sodium channel alpha-sub...    30  8.8

>YCT4_YEAST (P25625) Hypothetical 42.5 kDa protein in TSM1-ARE1
           intergenic region
          Length = 357

 Score =  118 bits (296), Expect = 2e-26
 Identities = 97/350 (27%), Positives = 155/350 (43%), Gaps = 56/350 (16%)

Query: 19  VIDASAGDVDPRYRDCIRQCQETGCVAQSCF----PHCKFPSDGEHFDRPWYMQEPLYLQ 74
           ++  S GD    + DC   C+       S      P      D E FD P     PLY +
Sbjct: 15  LVTCSPGDNLDEFIDCTYACEYNRRCPNSQINYIDPETNMFHDIEFFDTP-----PLYSK 69

Query: 75  WKKWDCQNDCRYHCM-------LDREKERKLLNLVPVKYHGKWPFARIYGMQEAASVAFS 127
              WDC +DC Y C        +D E+E         ++HGKWPF R+ G QE  S  FS
Sbjct: 70  LLFWDCISDCDYQCQHIITRWRIDEEEEI-------YQFHGKWPFLRVLGTQEFFSTIFS 122

Query: 128 ALNLAMHFHGWVSFFILLYYKLPLKDGKEAYYEYAGLW-HIY-GLLSLNSWFWSAVFHSR 185
             N   H+ G+V F  ++  +    D +        +W ++Y  +  + +W  S+VFH R
Sbjct: 123 IGNFIPHYKGFVKFSRIIREE---GDRRRKNSRSILIWNYLYVTVAGMLAWTASSVFHCR 179

Query: 186 DVDLTETLDY--SSAVILLGYSLILAILRSFNVRDEATRVMVSAPLIAFVITHVMYINFY 243
           D+ +TE LDY  +   +L G+  I A + S  +  +  +   +A + A    H++ + + 
Sbjct: 180 DLIITEKLDYFFAGLTVLTGFHAIFARMTSMFLYPKIAQAF-TASVAAIFALHILRL-YV 237

Query: 244 KLDYGWNMIVCVVMAVVQLVIWAVWAGLSGHP-SRWKLW--------------------- 281
              Y +NM   +   V+Q ++  + +  + H   + KL                      
Sbjct: 238 DWSYTYNMRFNIFFGVLQYILLIMLSCQNYHALQKQKLMGEFKKTAYSSFKRQIFKLCVI 297

Query: 282 --LVVIAGGLAMLLEIYDFPPYEGLLDAHALWHATTIPLTYIWWSFIRDD 329
             L+VI   +AM LE++DF  YE  +DAHALWH  TI  +++ + F  +D
Sbjct: 298 PILLVIVTTMAMSLELFDFFSYEWQIDAHALWHLCTIWPSWVLYDFFLED 347


>YEJM_ECOLI (P33922) Hypothetical protein yejM
          Length = 586

 Score = 31.6 bits (70), Expect = 3.0
 Identities = 25/85 (29%), Positives = 39/85 (45%), Gaps = 11/85 (12%)

Query: 171 LSLNSWFWSAVFHSRDVDLTET--LDYSSAVILLGYSLILAI-----LRSFNVRDEATRV 223
           L LN   W  V +  + ++     L + S  ++L   L+ A      LRS   R    R 
Sbjct: 113 LHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKLRSLTRRRRFARP 172

Query: 224 MVSAPLIAFVITHVMYI----NFYK 244
           + +   IAF+ +HV+YI    NFY+
Sbjct: 173 LAAFLFIAFIASHVVYIWADANFYR 197


>RIR2_YEAST (P09938) Ribonucleoside-diphosphate reductase small
           chain 1 (EC 1.17.4.1) (Ribonucleotide reductase small
           subunit)
          Length = 399

 Score = 31.6 bits (70), Expect = 3.0
 Identities = 19/59 (32%), Positives = 27/59 (45%), Gaps = 10/59 (16%)

Query: 87  HCMLDREKERKLLN-------LVPVKYHGKWPFARIYGMQEAASVAFSALNLAMHFHGW 138
           H + + EKE  LLN       L P+KYH  W   + Y   EA+      ++L+   H W
Sbjct: 67  HKLKEMEKEEPLLNEDKERTVLFPIKYHEIW---QAYKRAEASFWTAEEIDLSKDIHDW 122


>HLY3_BACSU (P54175) Hemolysin III homolog
          Length = 213

 Score = 31.6 bits (70), Expect = 3.0
 Identities = 36/152 (23%), Positives = 63/152 (40%), Gaps = 23/152 (15%)

Query: 170 LLSLNSWFWSAVFHSRDVDLTETLDYSSAVILLGYSLILAILRSFNVRDEATRVMVSAPL 229
           LL L+S    ++ H +  D+ E +D+S+  +L+  +    +L         T +++   L
Sbjct: 53  LLYLSSTLLHSITHKKTKDILEIIDHSAIYVLIAGTYTPFLLGPLKGTLGFTLLVIVWSL 112

Query: 230 --------IAFVITHVMYINFYKLDYGWNMIVCVVMAVVQLVIWAVWAGLSGHPSRWKLW 281
                   I FV   ++   F  L  GW MI+ V           ++A LSG      L+
Sbjct: 113 ALGGIVFKIFFVKRFILLSTFVYLVMGWLMIIAVK---------PLYASLSG-AGFSLLF 162

Query: 282 LVVIAGGLAMLLEIYDFPPYEGLLDAHALWHA 313
           L  I   +  +  I+   P+      HA+WH+
Sbjct: 163 LGGILYSVGTIFYIWKKIPFH-----HAIWHS 189


>BOSS_DROVI (Q24738) Bride of sevenless protein precursor
          Length = 893

 Score = 31.2 bits (69), Expect = 3.9
 Identities = 18/47 (38%), Positives = 28/47 (59%), Gaps = 3/47 (6%)

Query: 252 IVCVVMAVVQLVIWAVWAGLSGHPSRWK---LWLVVIAGGLAMLLEI 295
           I+ V+ AV+ L+IW+VW  LS     W+   + L + A G A+L+ I
Sbjct: 723 ILIVIGAVLILIIWSVWIALSMFGDEWRDAAIPLGMQASGWAVLVGI 769


>YNI7_YEAST (P48231) Hypothetical 132.5 kDa protein in TOP2-MKT1
           intergenic region
          Length = 1178

 Score = 30.8 bits (68), Expect = 5.2
 Identities = 16/47 (34%), Positives = 28/47 (59%), Gaps = 4/47 (8%)

Query: 68  QEPLYLQWKK---WDCQNDCRYHCML-DREKERKLLNLVPVKYHGKW 110
           Q+P Y+ WK+   W+ +++     +L D  K+  L NL+P K++G W
Sbjct: 53  QDPSYVGWKQVGGWEEKDELTSEDLLVDVNKDTFLGNLLPDKFYGDW 99


>YCX1_ODOSI (P49827) Hypothetical 41.6 kDa protein in psaA-ycf32.1
           and ycf32.2-acpP intergenic regions (ORF355)
          Length = 355

 Score = 30.4 bits (67), Expect = 6.7
 Identities = 27/116 (23%), Positives = 52/116 (44%), Gaps = 23/116 (19%)

Query: 117 GMQEAASVAFSALNLAMHFHGWVSFFILLYYKLPLKDGKEAYYEYAGLWHIYGLLSLNSW 176
           G++   +  FS L   ++F+ +    I+L Y + +         Y   W +Y L+SL  W
Sbjct: 128 GLETIRNTLFSML---VYFYFFAELRIMLSYFISINP-------YTRPW-VY-LISLTDW 175

Query: 177 FWSAVFHSRDVDLTETLDYSSAVILLGYSLILAILRSF--NVRDEATRVMVSAPLI 230
            +  +FH         L  S  V+LLG+ L+  ++ +   N+ D    ++ + P +
Sbjct: 176 IYDILFH---------LGISKRVVLLGFPLLPILIHAALGNLIDSLNHLVFTMPFL 222


>TME8_MOUSE (Q9ESN3) Transmembrane protein 8 precursor (M83 protein)
          Length = 769

 Score = 30.4 bits (67), Expect = 6.7
 Identities = 17/71 (23%), Positives = 33/71 (45%), Gaps = 7/71 (9%)

Query: 249 WNMI-VCVVMAVVQLVIWAVWAGLSG--HPSRWKLWLVVIAGGLAML---LEIY-DFPPY 301
           WN++  CV   V+   +W    G  G  +P+ W+ W+  +  G++M    + +Y      
Sbjct: 654 WNLMGPCVFAFVIMASMWIYRCGHRGQCYPTSWQRWVFYLLPGISMASVGIAMYTSMMTS 713

Query: 302 EGLLDAHALWH 312
           +     H++WH
Sbjct: 714 DNYYYTHSIWH 724


>GLTS_HAEIN (P45240) Sodium/glutamate symport carrier protein
           (Glutamate permease)
          Length = 404

 Score = 30.4 bits (67), Expect = 6.7
 Identities = 26/108 (24%), Positives = 55/108 (50%), Gaps = 18/108 (16%)

Query: 191 ETLDYSSAVILLGYSLI--LAILRSFNVRDEATRVMVSAPLIAFVITHVMYINFYKLDYG 248
           ETL  +S V+LLGY L+  + +L++FN+ +         P++   I  +  + ++K+D G
Sbjct: 9   ETLALASLVLLLGYFLVKRINVLKTFNIPE---------PVVGGFIVAIGLLIWHKID-G 58

Query: 249 WNMIVCVVMAVVQLVIWAVWAGLSGHPSRWKLWLVVIAGGLAMLLEIY 296
            +      +    ++++    GLS + SR      +I GG  +++ ++
Sbjct: 59  TSFNFDKNLQTTMMLVFFTSIGLSANFSR------LIKGGKPLVVFLF 100


>SCAA_XENLA (P51167) Amiloride-sensitive sodium channel
           alpha-subunit (Epithelial Na+ channel alpha subunit)
           (Alpha ENaC) (Nonvoltage-gated sodium channel 1 alpha
           subunit) (SCNEA) (Alpha NaCH)
          Length = 632

 Score = 30.0 bits (66), Expect = 8.8
 Identities = 48/221 (21%), Positives = 85/221 (37%), Gaps = 34/221 (15%)

Query: 34  CIRQCQETGCVAQSCFPHCKFP-SDGEHF-----DRPW---YMQEPLYLQWKKWDCQNDC 84
           C+R C +   VA+    +  +P S G+ +      + W   Y +  +     K  C   C
Sbjct: 377 CVRSCFQAAMVARCGCGYAFYPLSPGDQYCDYNKHKSWGHCYYKLIIEFTSNKLGCFTKC 436

Query: 85  RYHCMLDREKERKLLNLVPVKYHGKW---PFARIYGMQEAASVAFSALNLAMHFHGWVSF 141
           R  C++   +     +  P +    W     +R Y + +   +A   LN+         +
Sbjct: 437 RKPCLVSEYQLTAGYSKWPNRVSQDWVLHTLSRQYNLTDRNGIA--KLNI---------Y 485

Query: 142 FILLYYKLPLKDGKEAYYEYAGLWHIYGLLSLNSWFWSAVFHSRDVDLTETLDYSSAVIL 201
           F  L YK  L+         +   ++  LLSL    WS  F S  + + E L+     ++
Sbjct: 486 FEELNYKTILE---------SPTINMAMLLSLLGSQWSLWFGSSVLSVVEMLELVIDFVI 536

Query: 202 LGYSLIL--AILRSFNVRDEATRVMVSAPLIAFVITHVMYI 240
           +G  ++L     +  N  +E T V   AP  A +   V +I
Sbjct: 537 IGVMILLHRYYYKKANEGEETTVVPTPAPAFADLEQQVPHI 577


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.328    0.142    0.485 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,709,739
Number of Sequences: 164201
Number of extensions: 1808256
Number of successful extensions: 4609
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 4604
Number of HSP's gapped (non-prelim): 11
length of query: 342
length of database: 59,974,054
effective HSP length: 111
effective length of query: 231
effective length of database: 41,747,743
effective search space: 9643728633
effective search space used: 9643728633
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 66 (30.0 bits)