BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0166.14
(286 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CTR1_ARATH (Q05609) Serine/threonine-protein kinase CTR1 (EC 2.7... 157 4e-38
KYK2_DICDI (P18161) Tyrosine-protein kinase 2 (EC 2.7.1.112) (Fr... 154 2e-37
M3KC_RAT (Q63796) Mitogen-activated protein kinase kinase kinase... 146 6e-35
M3KC_MOUSE (Q60700) Mitogen-activated protein kinase kinase kina... 146 6e-35
M3KC_HUMAN (Q12852) Mitogen-activated protein kinase kinase kina... 146 6e-35
KYK1_DICDI (P18160) Non-receptor tyrosine kinase spore lysis A (... 144 3e-34
M3KA_HUMAN (Q02779) Mitogen-activated protein kinase kinase kina... 140 5e-33
RMIL_AVII1 (P10533) Serine/threonine-protein kinase transforming... 137 2e-32
RMIL_AVEVR (P27966) Serine/threonine-protein kinase transforming... 137 2e-32
BRAF_MOUSE (P28028) B-Raf proto-oncogene serine/threonine-protei... 137 2e-32
BRAF_HUMAN (P15056) B-Raf proto-oncogene serine/threonine-protei... 137 2e-32
BRAF_COTJA (P34908) B-Raf proto-oncogene serine/threonine-protei... 137 2e-32
BRAF_CHICK (Q04982) B-Raf proto-oncogene serine/threonine-protei... 137 2e-32
M3K9_HUMAN (P80192) Mitogen-activated protein kinase kinase kina... 131 2e-30
RAF1_RAT (P11345) RAF proto-oncogene serine/threonine-protein ki... 131 2e-30
RAF1_HUMAN (P04049) RAF proto-oncogene serine/threonine-protein ... 131 2e-30
RAF1_CHICK (P05625) RAF proto-oncogene serine/threonine-protein ... 130 4e-30
MIL_AVIMH (P00531) Serine/threonine-protein kinase transforming ... 128 1e-29
RAF_MSV36 (P00532) Serine/threonine-protein kinase transforming ... 128 2e-29
RAF1_MOUSE (Q99N57) RAF proto-oncogene serine/threonine-protein ... 128 2e-29
>CTR1_ARATH (Q05609) Serine/threonine-protein kinase CTR1 (EC
2.7.1.37)
Length = 821
Score = 157 bits (396), Expect = 4e-38
Identities = 87/220 (39%), Positives = 131/220 (59%), Gaps = 8/220 (3%)
Query: 62 LLIGMKFASGRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQFTSEVSLLLPLRHP 121
L I K +G +++ + DVA+K++ + +D +F EV+++ LRHP
Sbjct: 551 LNIKEKIGAGSFGTVHRAEWHGSDVAVKILME----QDFHAERVNEFLREVAIMKRLRHP 606
Query: 122 NIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHS-VPLHLVLKLALDIARGMQYLH 180
NI+ F+ A +PP I+TEYL+ GSL + LH + L +A D+A+GM YLH
Sbjct: 607 NIVLFMGAVTQPPNLSIVTEYLSRGSLYRLLHKSGAREQLDERRRLSMAYDVAKGMNYLH 666
Query: 181 SQG--IIHRDLKSENLLLGEDMCVKVVDFGISCLESQCG-SAKGFTGTYRWMAPEMIKEK 237
++ I+HRDLKS NLL+ + VKV DFG+S L++ S+K GT WMAPE+++++
Sbjct: 667 NRNPPIVHRDLKSPNLLVDKKYTVKVCDFGLSRLKASTFLSSKSAAGTPEWMAPEVLRDE 726
Query: 238 HHTKKVDVYSFGIVLWELLTGLTPFDNMTPEQAAYAVSYK 277
+K DVYSFG++LWEL T P+ N+ P Q AV +K
Sbjct: 727 PSNEKSDVYSFGVILWELATLQQPWGNLNPAQVVAAVGFK 766
>KYK2_DICDI (P18161) Tyrosine-protein kinase 2 (EC 2.7.1.112)
(Fragment)
Length = 410
Score = 154 bits (389), Expect = 2e-37
Identities = 98/260 (37%), Positives = 146/260 (55%), Gaps = 15/260 (5%)
Query: 26 SLGEYKRAVSWSKYLVSPGAAIKGE-GEEEWSADMSQLLIGMKFASGRHSRIYKGVYKER 84
SL + +RA+ K + +K GE E+ D++ + K G S +++G +K
Sbjct: 71 SLNDIQRAIESEKIKKTKFEELKSILGEREYIIDINDIQFIQKVGEGAFSEVWEGWWKGI 130
Query: 85 DVAIKLVSQPEEDEDLACFLEKQFTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLA 144
VAIK + ++E +++F EV L H NI+ F+ AC KP CIITEY+A
Sbjct: 131 HVAIKKLKIIGDEEQF----KERFIREVQNLKKGNHQNIVMFIGACYKPA--CIITEYMA 184
Query: 145 GGSLRKYLHHQEPHSVPLH----LVLKLALDIARGMQYLHSQGIIHRDLKSENLLLGEDM 200
GGSL LH+ + + LVLK+A D+A G+ +LHS I+HRDL S+N+LL E
Sbjct: 185 GGSLYNILHNPNSSTPKVKYSFPLVLKMATDMALGLLHLHSITIVHRDLTSQNILLDELG 244
Query: 201 CVKVVDFGISCLESQCGS---AKGFTGTYRWMAPEMIKE-KHHTKKVDVYSFGIVLWELL 256
+K+ DFG+S +S+ GS G RW PE+ K H+++KVDVY F +V+WE+L
Sbjct: 245 NIKISDFGLSAEKSREGSMTMTNGGICNPRWRPPELTKNLGHYSEKVDVYCFSLVVWEIL 304
Query: 257 TGLTPFDNMTPEQAAYAVSY 276
TG PF ++ Q + V+Y
Sbjct: 305 TGEIPFSDLDGSQRSAQVAY 324
>M3KC_RAT (Q63796) Mitogen-activated protein kinase kinase kinase 12
(EC 2.7.1.37) (MAPK-upstream kinase) (MUK)
Length = 888
Score = 146 bits (368), Expect = 6e-35
Identities = 83/227 (36%), Positives = 122/227 (53%), Gaps = 15/227 (6%)
Query: 48 KGEGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQ 107
K + E+ W ++L SG ++ G + +VA+K V +E
Sbjct: 144 KQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKE----------- 192
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
+++ L L+HPNIITF C + P +CI+ E+ A G L + L P V L++
Sbjct: 193 --TDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRP--VTPSLLVD 248
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGFTGTYR 227
++ IA GM YLH IIHRDLKS N+L+ D VK+ DFG S S + F GT
Sbjct: 249 WSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVA 308
Query: 228 WMAPEMIKEKHHTKKVDVYSFGIVLWELLTGLTPFDNMTPEQAAYAV 274
WMAPE+I+ + ++KVD++SFG+VLWELLTG P+ ++ + V
Sbjct: 309 WMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGV 355
>M3KC_MOUSE (Q60700) Mitogen-activated protein kinase kinase kinase
12 (EC 2.7.1.37) (Leucine-zipper protein kinase) (ZPK)
(Dual leucine zipper bearing kinase) (DLK)
Length = 888
Score = 146 bits (368), Expect = 6e-35
Identities = 83/227 (36%), Positives = 122/227 (53%), Gaps = 15/227 (6%)
Query: 48 KGEGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQ 107
K + E+ W ++L SG ++ G + +VA+K V +E
Sbjct: 144 KQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKE----------- 192
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
+++ L L+HPNIITF C + P +CI+ E+ A G L + L P V L++
Sbjct: 193 --TDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRP--VTPSLLVD 248
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGFTGTYR 227
++ IA GM YLH IIHRDLKS N+L+ D VK+ DFG S S + F GT
Sbjct: 249 WSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVA 308
Query: 228 WMAPEMIKEKHHTKKVDVYSFGIVLWELLTGLTPFDNMTPEQAAYAV 274
WMAPE+I+ + ++KVD++SFG+VLWELLTG P+ ++ + V
Sbjct: 309 WMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGV 355
>M3KC_HUMAN (Q12852) Mitogen-activated protein kinase kinase kinase
12 (EC 2.7.1.37) (Leucine-zipper protein kinase) (ZPK)
Length = 859
Score = 146 bits (368), Expect = 6e-35
Identities = 83/227 (36%), Positives = 122/227 (53%), Gaps = 15/227 (6%)
Query: 48 KGEGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQ 107
K + E+ W ++L SG ++ G + +VA+K V +E
Sbjct: 111 KQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKE----------- 159
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
+++ L L+HPNIITF C + P +CI+ E+ A G L + L P V L++
Sbjct: 160 --TDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRP--VTPSLLVD 215
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGFTGTYR 227
++ IA GM YLH IIHRDLKS N+L+ D VK+ DFG S S + F GT
Sbjct: 216 WSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVA 275
Query: 228 WMAPEMIKEKHHTKKVDVYSFGIVLWELLTGLTPFDNMTPEQAAYAV 274
WMAPE+I+ + ++KVD++SFG+VLWELLTG P+ ++ + V
Sbjct: 276 WMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGV 322
>KYK1_DICDI (P18160) Non-receptor tyrosine kinase spore lysis A (EC
2.7.1.112) (Tyrosine-protein kinase 1)
Length = 1584
Score = 144 bits (362), Expect = 3e-34
Identities = 90/244 (36%), Positives = 136/244 (54%), Gaps = 24/244 (9%)
Query: 54 EWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQFTSEVS 113
E+ D ++L G G + +G ++E DVAIK++ + + + + F +EV
Sbjct: 1281 EYEIDFNELEFGQTIGKGFFGEVKRGYWRETDVAIKIIYRDQFKTKSSLVM---FQNEVG 1337
Query: 114 LLLPLRHPNIITFVAACKK--PPVFCIITEYLAGGSLRKYL--HHQEPHSVPLHLVLKLA 169
+L LRHPN++ F+ AC CI+TE++ GGSLR++L H P H+ LKLA
Sbjct: 1338 ILSKLRHPNVVQFLGACTAGGEDHHCIVTEWMGGGSLRQFLTDHFNLLEQNP-HIRLKLA 1396
Query: 170 LDIARGMQYLH--SQGIIHRDLKSENLLLG-------------EDMCVKVVDFGISCLES 214
LDIA+GM YLH + I+HRDL S N+LL +D+ K+ DFG+S L+
Sbjct: 1397 LDIAKGMNYLHGWTPPILHRDLSSRNILLDHNIDPKNPVVSSRQDIKCKISDFGLSRLKK 1456
Query: 215 -QCGSAKGFTGTYRWMAPEMIKEKHHTKKVDVYSFGIVLWELLTGLTPFDNMTPEQAAYA 273
Q G +MAPE+ K +++K DVYS+G+VL+ELLT P +M P + A+
Sbjct: 1457 EQASQMTQSVGCIPYMAPEVFKGDSNSEKSDVYSYGMVLFELLTSDEPQQDMKPMKMAHL 1516
Query: 274 VSYK 277
+Y+
Sbjct: 1517 AAYE 1520
>M3KA_HUMAN (Q02779) Mitogen-activated protein kinase kinase kinase
10 (EC 2.7.1.37) (Mixed lineage kinase 2) (Protein
kinase MST)
Length = 954
Score = 140 bits (352), Expect = 5e-33
Identities = 82/217 (37%), Positives = 121/217 (54%), Gaps = 19/217 (8%)
Query: 71 GRHSRIYKGVYKERDVAIKLVS-QPEEDEDLACFLEKQFTSEVSLLLPLRHPNIITFVAA 129
G ++Y+ +++ +VA+K PE+D + +Q E L L+HPNII A
Sbjct: 107 GGFGKVYRALWRGEEVAVKAARLDPEKDPAVTA---EQVCQEARLFGALQHPNIIALRGA 163
Query: 130 CKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLKLALDIARGMQYLHSQG---IIH 186
C PP C++ EY GG+L + L + VP H+++ A+ +ARGM YLH+ IIH
Sbjct: 164 CLNPPHLCLVMEYARGGALSRVLAGRR---VPPHVLVNWAVQVARGMNYLHNDAPVPIIH 220
Query: 187 RDLKSENLLLGE--------DMCVKVVDFGISCLESQCGSAKGFTGTYRWMAPEMIKEKH 238
RDLKS N+L+ E D +K+ DFG++ E + GTY WMAPE+I+
Sbjct: 221 RDLKSINILILEAIENHNLADTVLKITDFGLA-REWHKTTKMSAAGTYAWMAPEVIRLSL 279
Query: 239 HTKKVDVYSFGIVLWELLTGLTPFDNMTPEQAAYAVS 275
+K DV+SFG++LWELLTG P+ + AY V+
Sbjct: 280 FSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVA 316
>RMIL_AVII1 (P10533) Serine/threonine-protein kinase transforming
protein Rmil (EC 2.7.1.37)
Length = 367
Score = 137 bits (346), Expect = 2e-32
Identities = 82/224 (36%), Positives = 131/224 (57%), Gaps = 17/224 (7%)
Query: 50 EGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQ 107
+ ++W Q+ +G + SG +YKG + DVA+K+ V+ P + A
Sbjct: 55 DSSDDWEIPDGQITVGQRIGSGSFGTVYKGKW-HGDVAVKMLNVTAPTPQQLQA------ 107
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
F +EV +L RH NI+ F+ KP + I+T++ G SL +LH E + L+
Sbjct: 108 FKNEVGVLRKTRHVNILLFMGYSTKPQL-AIVTQWCEGSSLYHHLHIIETKFEMIKLI-D 165
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TG 224
+A A+GM YLH++ IIHRDLKS N+ L ED+ VK+ DFG++ ++S+ + F +G
Sbjct: 166 IARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSG 225
Query: 225 TYRWMAPEMIK---EKHHTKKVDVYSFGIVLWELLTGLTPFDNM 265
+ WMAPE+I+ + ++ + DVY+FGIVL+EL+TG P+ N+
Sbjct: 226 SILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNI 269
>RMIL_AVEVR (P27966) Serine/threonine-protein kinase transforming
protein Rmil (EC 2.7.1.37)
Length = 450
Score = 137 bits (346), Expect = 2e-32
Identities = 82/224 (36%), Positives = 131/224 (57%), Gaps = 17/224 (7%)
Query: 50 EGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQ 107
+ ++W Q+ +G + SG +YKG + DVA+K+ V+ P + A
Sbjct: 71 DSSDDWEIPDGQITVGQRIGSGSFGTVYKGKW-HGDVAVKMLNVTAPTPQQLQA------ 123
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
F +EV +L RH NI+ F+ KP + I+T++ G SL +LH E + L+
Sbjct: 124 FKNEVGVLRKTRHVNILLFMGYSTKPQL-AIVTQWCEGSSLYHHLHIIETKFEMIKLI-D 181
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TG 224
+A A+GM YLH++ IIHRDLKS N+ L ED+ VK+ DFG++ ++S+ + F +G
Sbjct: 182 IARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSG 241
Query: 225 TYRWMAPEMIK---EKHHTKKVDVYSFGIVLWELLTGLTPFDNM 265
+ WMAPE+I+ + ++ + DVY+FGIVL+EL+TG P+ N+
Sbjct: 242 SILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNI 285
>BRAF_MOUSE (P28028) B-Raf proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (Fragment)
Length = 328
Score = 137 bits (346), Expect = 2e-32
Identities = 82/224 (36%), Positives = 131/224 (57%), Gaps = 17/224 (7%)
Query: 50 EGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQ 107
+ ++W Q+ +G + SG +YKG + DVA+K+ V+ P + A
Sbjct: 7 DSSDDWEIPDGQITVGQRIGSGSFGTVYKGKW-HGDVAVKMLNVTAPTPQQLQA------ 59
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
F +EV +L RH NI+ F+ KP + I+T++ G SL +LH E + L+
Sbjct: 60 FKNEVGVLRKTRHVNILLFMGYSTKPQL-AIVTQWCEGSSLYHHLHIIETKFEMIKLI-D 117
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TG 224
+A A+GM YLH++ IIHRDLKS N+ L ED+ VK+ DFG++ ++S+ + F +G
Sbjct: 118 IARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSG 177
Query: 225 TYRWMAPEMIK---EKHHTKKVDVYSFGIVLWELLTGLTPFDNM 265
+ WMAPE+I+ + ++ + DVY+FGIVL+EL+TG P+ N+
Sbjct: 178 SILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNI 221
>BRAF_HUMAN (P15056) B-Raf proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (p94) (v-Raf murine sarcoma viral
oncogene homolog B1)
Length = 766
Score = 137 bits (346), Expect = 2e-32
Identities = 82/224 (36%), Positives = 131/224 (57%), Gaps = 17/224 (7%)
Query: 50 EGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQ 107
+ ++W Q+ +G + SG +YKG + DVA+K+ V+ P + A
Sbjct: 445 DSSDDWEIPDGQITVGQRIGSGSFGTVYKGKW-HGDVAVKMLNVTAPTPQQLQA------ 497
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
F +EV +L RH NI+ F+ KP + I+T++ G SL +LH E + L+
Sbjct: 498 FKNEVGVLRKTRHVNILLFMGYSTKPQL-AIVTQWCEGSSLYHHLHIIETKFEMIKLI-D 555
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TG 224
+A A+GM YLH++ IIHRDLKS N+ L ED+ VK+ DFG++ ++S+ + F +G
Sbjct: 556 IARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSG 615
Query: 225 TYRWMAPEMIK---EKHHTKKVDVYSFGIVLWELLTGLTPFDNM 265
+ WMAPE+I+ + ++ + DVY+FGIVL+EL+TG P+ N+
Sbjct: 616 SILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNI 659
>BRAF_COTJA (P34908) B-Raf proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (RMIL serine/threonine-protein
kinase) (c-RMIL)
Length = 807
Score = 137 bits (346), Expect = 2e-32
Identities = 82/224 (36%), Positives = 131/224 (57%), Gaps = 17/224 (7%)
Query: 50 EGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQ 107
+ ++W Q+ +G + SG +YKG + DVA+K+ V+ P + A
Sbjct: 485 DSSDDWEIPDGQITVGQRIGSGSFGTVYKGKW-HGDVAVKMLNVTAPTPQQLQA------ 537
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
F +EV +L RH NI+ F+ KP + I+T++ G SL +LH E + L+
Sbjct: 538 FKNEVGVLRKTRHVNILLFMGYSTKPQL-AIVTQWCEGSSLYHHLHIIETKFEMIKLI-D 595
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TG 224
+A A+GM YLH++ IIHRDLKS N+ L ED+ VK+ DFG++ ++S+ + F +G
Sbjct: 596 IARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSG 655
Query: 225 TYRWMAPEMIK---EKHHTKKVDVYSFGIVLWELLTGLTPFDNM 265
+ WMAPE+I+ + ++ + DVY+FGIVL+EL+TG P+ N+
Sbjct: 656 SILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNI 699
>BRAF_CHICK (Q04982) B-Raf proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (RMIL serine/threonine-protein
kinase) (c-RMIL)
Length = 806
Score = 137 bits (346), Expect = 2e-32
Identities = 82/224 (36%), Positives = 131/224 (57%), Gaps = 17/224 (7%)
Query: 50 EGEEEWSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQ 107
+ ++W Q+ +G + SG +YKG + DVA+K+ V+ P + A
Sbjct: 485 DSSDDWEIPDGQITVGQRIGSGSFGTVYKGKW-HGDVAVKMLNVTAPTPQQLQA------ 537
Query: 108 FTSEVSLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLK 167
F +EV +L RH NI+ F+ KP + I+T++ G SL +LH E + L+
Sbjct: 538 FKNEVGVLRKTRHVNILLFMGYSTKPQL-AIVTQWCEGSSLYHHLHIIETKFEMIKLI-D 595
Query: 168 LALDIARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TG 224
+A A+GM YLH++ IIHRDLKS N+ L ED+ VK+ DFG++ ++S+ + F +G
Sbjct: 596 IARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSG 655
Query: 225 TYRWMAPEMIK---EKHHTKKVDVYSFGIVLWELLTGLTPFDNM 265
+ WMAPE+I+ + ++ + DVY+FGIVL+EL+TG P+ N+
Sbjct: 656 SILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNI 699
>M3K9_HUMAN (P80192) Mitogen-activated protein kinase kinase kinase
9 (EC 2.7.1.37) (Mixed lineage kinase 1) (Fragment)
Length = 394
Score = 131 bits (330), Expect = 2e-30
Identities = 77/216 (35%), Positives = 120/216 (54%), Gaps = 17/216 (7%)
Query: 71 GRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQFTSEVSLLLPLRHPNIITFVAAC 130
G ++Y+ + +VA+K ++ + DED++ +E E L L+HPNII C
Sbjct: 12 GGFGKVYRAFWIGDEVAVK-AARHDPDEDISQTIEN-VRQEAKLFAMLKHPNIIALRGVC 69
Query: 131 KKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLKLALDIARGMQYLHSQGI---IHR 187
K P C++ E+ GG L + L + +P +++ A+ IARGM YLH + I IHR
Sbjct: 70 LKEPNLCLVMEFARGGPLNRVLSGKR---IPPDILVNWAVQIARGMNYLHDEAIVPIIHR 126
Query: 188 DLKSENLLLGEDM--------CVKVVDFGISCLESQCGSAKGFTGTYRWMAPEMIKEKHH 239
DLKS N+L+ + + +K+ DFG++ E + GTY WMAPE+I+
Sbjct: 127 DLKSSNILILQKVENGDLSNKILKITDFGLA-REWHRTTKMSAAGTYAWMAPEVIRASMF 185
Query: 240 TKKVDVYSFGIVLWELLTGLTPFDNMTPEQAAYAVS 275
+K DV+S+G++LWELLTG PF + + AY V+
Sbjct: 186 SKGSDVWSYGVLLWELLTGEVPFRGIDGLRVAYGVA 221
>RAF1_RAT (P11345) RAF proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (Raf-1) (C-RAF) (cRaf)
Length = 648
Score = 131 bits (329), Expect = 2e-30
Identities = 76/217 (35%), Positives = 128/217 (58%), Gaps = 13/217 (5%)
Query: 55 WSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQFTSEVSL 114
W + S++++ + SG +YKG + DVA+K++ + + + F +EV++
Sbjct: 342 WEIEASEVMLSTRIGSGSFGTVYKGKW-HGDVAVKILKVVDPTPEQL----QAFRNEVAV 396
Query: 115 LLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLKLALDIAR 174
L RH NI+ F+ K + I+T++ G SL K+LH QE L+ +A A+
Sbjct: 397 LRKTRHVNILLFMGYMTKDNL-AIVTQWCEGSSLYKHLHVQETKFQMFQLI-DIARQTAQ 454
Query: 175 GMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TGTYRWMAP 231
GM YLH++ IIHRD+KS N+ L E + VK+ DFG++ ++S+ ++ TG+ WMAP
Sbjct: 455 GMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAP 514
Query: 232 EMIKEKHHTK---KVDVYSFGIVLWELLTGLTPFDNM 265
E+I+ + + + DVYS+GIVL+EL+TG P+ ++
Sbjct: 515 EVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHI 551
>RAF1_HUMAN (P04049) RAF proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (Raf-1) (C-RAF) (cRaf)
Length = 648
Score = 131 bits (329), Expect = 2e-30
Identities = 79/219 (36%), Positives = 129/219 (58%), Gaps = 17/219 (7%)
Query: 55 WSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQFTSEV 112
W + S++++ + SG +YKG + DVA+K+ V P ++ A F +EV
Sbjct: 342 WEIEASEVMLSTRIGSGSFGTVYKGKW-HGDVAVKILKVVDPTPEQFQA------FRNEV 394
Query: 113 SLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLKLALDI 172
++L RH NI+ F+ K + I+T++ G SL K+LH QE L+ +A
Sbjct: 395 AVLRKTRHVNILLFMGYMTKDNL-AIVTQWCEGSSLYKHLHVQETKFQMFQLI-DIARQT 452
Query: 173 ARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TGTYRWM 229
A+GM YLH++ IIHRD+KS N+ L E + VK+ DFG++ ++S+ ++ TG+ WM
Sbjct: 453 AQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWM 512
Query: 230 APEMIKEKHHTK---KVDVYSFGIVLWELLTGLTPFDNM 265
APE+I+ + + + DVYS+GIVL+EL+TG P+ ++
Sbjct: 513 APEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHI 551
>RAF1_CHICK (P05625) RAF proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (RAF-1) (C-RAF) (MIL proto-oncogene
serine/threonine-protein kinase) (C-MIL)
Length = 647
Score = 130 bits (327), Expect = 4e-30
Identities = 79/219 (36%), Positives = 128/219 (58%), Gaps = 17/219 (7%)
Query: 55 WSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQFTSEV 112
W + S++++ + SG +YKG + DVA+K+ V P ++ A F +EV
Sbjct: 342 WEIEASEVMLSTRIGSGSFGTVYKGKW-HGDVAVKILKVVDPTPEQFQA------FRNEV 394
Query: 113 SLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLKLALDI 172
++L RH NI+ F+ K + I+T++ G SL K+LH QE L+ +A
Sbjct: 395 AVLRKTRHVNILLFMGYMTKDNL-AIVTQWCEGSSLYKHLHVQETKFQMFQLI-DIARQT 452
Query: 173 ARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TGTYRWM 229
A+GM YLH++ IIHRD+KS N+ L E + VK+ DFG++ ++S+ ++ TG+ WM
Sbjct: 453 AQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSILWM 512
Query: 230 APEMIKEKHHTK---KVDVYSFGIVLWELLTGLTPFDNM 265
APE+I+ + + DVYS+GIVL+EL+TG P+ ++
Sbjct: 513 APEVIRMQDSNPFSFQSDVYSYGIVLYELMTGELPYSHI 551
>MIL_AVIMH (P00531) Serine/threonine-protein kinase transforming
protein mil (EC 2.7.1.37)
Length = 380
Score = 128 bits (322), Expect = 1e-29
Identities = 79/219 (36%), Positives = 127/219 (57%), Gaps = 17/219 (7%)
Query: 55 WSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKL--VSQPEEDEDLACFLEKQFTSEV 112
W + S++L+ + SG +YKG + DVA+K+ V P ++ A F +EV
Sbjct: 75 WEIEASEVLLSTRIGSGSFGTVYKGKW-HGDVAVKILKVVDPTPEQFQA------FRNEV 127
Query: 113 SLLLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLKLALDI 172
++L RH NI+ F+ K + I+T++ G SL K+LH QE L+ +A
Sbjct: 128 AVLRKTRHVNILLFMGYMTKDNL-AIVTQWCEGSSLYKHLHVQETKFQMFQLI-DIARQT 185
Query: 173 ARGMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TGTYRWM 229
A+GM YLH++ IIHRD+KS N+ L + VK+ DFG++ ++S+ ++ TG+ WM
Sbjct: 186 AQGMDYLHAKNIIHRDMKSNNIFLHGGLTVKIGDFGLATVKSRWSGSQQVEQPTGSILWM 245
Query: 230 APEMIKEKHHTK---KVDVYSFGIVLWELLTGLTPFDNM 265
APE+I+ + + DVYS+GIVL+EL+TG P+ ++
Sbjct: 246 APEVIRMQDSNPFSFQSDVYSYGIVLYELMTGELPYSHI 284
>RAF_MSV36 (P00532) Serine/threonine-protein kinase transforming
protein raf (EC 2.7.1.37)
Length = 323
Score = 128 bits (321), Expect = 2e-29
Identities = 75/214 (35%), Positives = 125/214 (58%), Gaps = 13/214 (6%)
Query: 55 WSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQFTSEVSL 114
W + S++++ + SG +YKG + DVA+K++ + + + F +EV++
Sbjct: 17 WKMEASEVMLSTRIGSGSFGTVYKGKW-HGDVAVKILKVVDPTPEQL----QAFRNEVAV 71
Query: 115 LLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLKLALDIAR 174
L RH NI+ F+ K + I+T++ G SL K+LH QE L+ +A A+
Sbjct: 72 LRKTRHVNILLFMGYMTKDNL-AIVTQWCEGSSLYKHLHVQETKFQMFQLI-DIARQTAQ 129
Query: 175 GMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TGTYRWMAP 231
GM YLH++ IIHRD+KS N+ L E + VK+ DFG++ ++S+ ++ TG+ WMAP
Sbjct: 130 GMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAP 189
Query: 232 EMIK---EKHHTKKVDVYSFGIVLWELLTGLTPF 262
E+I+ + + + DVYS+GIVL+EL+ G P+
Sbjct: 190 EVIRMQDDNPFSFQSDVYSYGIVLYELMAGELPY 223
>RAF1_MOUSE (Q99N57) RAF proto-oncogene serine/threonine-protein
kinase (EC 2.7.1.37) (Raf-1) (C-RAF) (cRaf)
Length = 648
Score = 128 bits (321), Expect = 2e-29
Identities = 75/214 (35%), Positives = 125/214 (58%), Gaps = 13/214 (6%)
Query: 55 WSADMSQLLIGMKFASGRHSRIYKGVYKERDVAIKLVSQPEEDEDLACFLEKQFTSEVSL 114
W + S++++ + SG +YKG + DVA+K++ + + + F +EV++
Sbjct: 342 WEIEASEVMLSTRIGSGSFGTVYKGKW-HGDVAVKILKVVDPTPEQL----QAFRNEVAV 396
Query: 115 LLPLRHPNIITFVAACKKPPVFCIITEYLAGGSLRKYLHHQEPHSVPLHLVLKLALDIAR 174
L RH NI+ F+ K + I+T++ G SL K+LH QE L+ +A A+
Sbjct: 397 LRKTRHVNILLFMGYMTKDNL-AIVTQWCEGSSLYKHLHVQETKFQMFQLI-DIARQTAQ 454
Query: 175 GMQYLHSQGIIHRDLKSENLLLGEDMCVKVVDFGISCLESQCGSAKGF---TGTYRWMAP 231
GM YLH++ IIHRD+KS N+ L E + VK+ DFG++ ++S+ ++ TG+ WMAP
Sbjct: 455 GMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAP 514
Query: 232 EMIK---EKHHTKKVDVYSFGIVLWELLTGLTPF 262
E+I+ + + + DVYS+GIVL+EL+ G P+
Sbjct: 515 EVIRMQDDNPFSFQSDVYSYGIVLYELMAGELPY 548
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.137 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,334,254
Number of Sequences: 164201
Number of extensions: 1635309
Number of successful extensions: 8665
Number of sequences better than 10.0: 1710
Number of HSP's better than 10.0 without gapping: 1596
Number of HSP's successfully gapped in prelim test: 114
Number of HSP's that attempted gapping in prelim test: 4387
Number of HSP's gapped (non-prelim): 1885
length of query: 286
length of database: 59,974,054
effective HSP length: 109
effective length of query: 177
effective length of database: 42,076,145
effective search space: 7447477665
effective search space used: 7447477665
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0166.14