BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0150.12
(266 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NRG1_CHICK (Q05199) Pro-neuregulin-1 precursor (Pro-NRG1) [Conta... 37 0.038
NRG1_HUMAN (Q02297) Pro-neuregulin-1 precursor (Pro-NRG1) [Conta... 37 0.050
NRG1_RAT (P43322) Pro-neuregulin-1 precursor (Pro-NRG1) [Contain... 36 0.11
KLFA_MOUSE (O89091) Transforming growth factor-beta-inducible ea... 35 0.19
KLFA_RAT (O08876) Transforming growth factor-beta-inducible earl... 35 0.25
PNK1_MOUSE (Q8K4K6) Pantothenate kinase 1 (EC 2.7.1.33) (Pantoth... 34 0.32
KLFA_HUMAN (Q13118) Transforming growth factor-beta-inducible ea... 33 0.72
PR33_DAUCA (P06600) Proline-rich 33 kDa extensin-related protein... 33 0.94
SUM1_YEAST (P46676) Suppressor of mar1-1 protein (SUM1-1 protein) 31 2.7
PGCA_CANFA (Q28343) Aggrecan core protein precursor (Cartilage-s... 31 3.6
CCAA_RAT (P54282) Voltage-dependent P/Q-type calcium channel alp... 31 3.6
ARR4_ARATH (O82798) Two-component response regulator ARR4 (Respo... 31 3.6
AK80_RAT (O35817) Testis-specific A-kinase-anchoring-protein TAK... 31 3.6
HXA3_HETFR (Q9IA21) Homeobox protein Hox-A3 30 4.7
EVI1_MOUSE (P14404) Ecotropic virus integration 1 site protein 30 4.7
CCAA_RABIT (P27884) Voltage-dependent P/Q-type calcium channel a... 30 4.7
CA1H_HUMAN (P39060) Collagen alpha 1(XVIII) chain precursor [Con... 30 4.7
YDQ2_SCHPO (O14196) Hypothetical protein C5D6.02c in chromosome I 30 6.1
WAS1_HUMAN (Q92558) Wiskott-Aldrich syndrome protein family memb... 30 6.1
MB11_COPCI (P40333) Mating-type protein beta1-1 30 6.1
>NRG1_CHICK (Q05199) Pro-neuregulin-1 precursor (Pro-NRG1)
[Contains: Neuregulin-1 (Acetylcholine receptor inducing
activity) (ARIA)]
Length = 602
Score = 37.4 bits (85), Expect = 0.038
Identities = 19/37 (51%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Query: 59 TTPTYMNP--FHVSLSPPSPPSTFEPPLASTNDSIDS 93
TTP M+P FH SP SPPS PP++S SI S
Sbjct: 392 TTPARMSPVDFHTPTSPKSPPSEMSPPVSSLTISIPS 428
>NRG1_HUMAN (Q02297) Pro-neuregulin-1 precursor (Pro-NRG1)
[Contains: Neuregulin-1 (Neu differentiation factor)
(Heregulin) (HRG) (Breast cancer cell differentiation
factor p45) (Acetylcholine receptor inducing activity)
(ARIA) (Sensory and motor neuron-d
Length = 639
Score = 37.0 bits (84), Expect = 0.050
Identities = 20/56 (35%), Positives = 27/56 (47%), Gaps = 2/56 (3%)
Query: 59 TTPTYMNP--FHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPPP 112
TTP M+P FH SP SPPS PP++S S+ S + ++ PP
Sbjct: 428 TTPARMSPVDFHTPSSPKSPPSEMSPPVSSMTVSMPSMAVSPFMEEERPLLLVTPP 483
>NRG1_RAT (P43322) Pro-neuregulin-1 precursor (Pro-NRG1) [Contains:
Neuregulin-1 (Neu differentiation factor) (Heregulin)
(HRG) (Acetylcholine receptor inducing activity) (ARIA)
(Sensory and motor neuron-derived factor) (Glial growth
factor)]
Length = 662
Score = 35.8 bits (81), Expect = 0.11
Identities = 18/37 (48%), Positives = 22/37 (58%), Gaps = 2/37 (5%)
Query: 59 TTPTYMNP--FHVSLSPPSPPSTFEPPLASTNDSIDS 93
TTP M+P FH SP SPPS PP++S S+ S
Sbjct: 451 TTPARMSPVDFHTPSSPKSPPSEMSPPVSSMTVSMPS 487
>KLFA_MOUSE (O89091) Transforming growth factor-beta-inducible early
growth response protein 1 (TGFB-inducible early growth
response protein 1) (TIEG-1) (Krueppel-like factor 10)
(GDNF inducible factor) (Transcription factor GIF)
(mGIF)
Length = 479
Score = 35.0 bits (79), Expect = 0.19
Identities = 37/121 (30%), Positives = 49/121 (39%), Gaps = 19/121 (15%)
Query: 61 PTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPPPFPWATDRR 120
P ++P LSP +P F P A IDSS RVR S I P
Sbjct: 330 PPVVSPSGTRLSPIAPAPGFSPSAARVTPQIDSS---RVR-----SHICSHP------GC 375
Query: 121 ARVYTLSHLLQNQIFTITGE----VQCKRCERKFEMGFDLKKNFSEVSSFIAKYECTMHD 176
+ Y S L+ + T TGE K CER+F +L ++ + K+ C M D
Sbjct: 376 GKTYFKSSHLKAHVRTHTGEKPFSCSWKGCERRFARSDELSRH-RRTHTGEKKFACPMCD 434
Query: 177 R 177
R
Sbjct: 435 R 435
>KLFA_RAT (O08876) Transforming growth factor-beta-inducible early
growth response protein 1 (TGFB-inducible early growth
response protein 1) (TIEG-1) (Krueppel-like factor 10)
(Zinc finger transcription factor homolog CPG20)
Length = 480
Score = 34.7 bits (78), Expect = 0.25
Identities = 37/121 (30%), Positives = 49/121 (39%), Gaps = 19/121 (15%)
Query: 61 PTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPPPFPWATDRR 120
P ++P LSP +P F P A IDSS RVR S I P
Sbjct: 331 PPVVSPNGTRLSPIAPAPGFSPSAARVTPQIDSS---RVR-----SHICSHP------GC 376
Query: 121 ARVYTLSHLLQNQIFTITGE----VQCKRCERKFEMGFDLKKNFSEVSSFIAKYECTMHD 176
+ Y S L+ + T TGE K CER+F +L ++ + K+ C M D
Sbjct: 377 GKTYFKSSHLKAHVRTHTGEKPFSCSWKGCERRFARSDELSRH-RRTHTGEKKFACPMCD 435
Query: 177 R 177
R
Sbjct: 436 R 436
>PNK1_MOUSE (Q8K4K6) Pantothenate kinase 1 (EC 2.7.1.33)
(Pantothenic acid kinase 1) (mPank1) (mPank)
Length = 548
Score = 34.3 bits (77), Expect = 0.32
Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 11/60 (18%)
Query: 65 NPFHVSLS----PPSP----PSTFEP-PLASTNDSIDSSSSRRVRRRVEKSEIIPPPFPW 115
N FH S + PP+P PS +P PL + +DS + R+RRR++ PPFPW
Sbjct: 131 NGFHPSAASSRDPPAPRFQLPSELQPQPLFAQHDS--PAKKCRLRRRMDSGRKNRPPFPW 188
>KLFA_HUMAN (Q13118) Transforming growth factor-beta-inducible early
growth response protein 1 (TGFB-inducible early growth
response protein 1) (TIEG-1) (Krueppel-like factor 10)
(EGRalpha)
Length = 480
Score = 33.1 bits (74), Expect = 0.72
Identities = 35/121 (28%), Positives = 46/121 (37%), Gaps = 19/121 (15%)
Query: 61 PTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPPPFPWATDRR 120
P ++P LSP +P F P A IDSS R S I P
Sbjct: 331 PPVVSPNGTRLSPIAPAPGFSPSAAKVTPQIDSSRIR--------SHICSHP------GC 376
Query: 121 ARVYTLSHLLQNQIFTITGE----VQCKRCERKFEMGFDLKKNFSEVSSFIAKYECTMHD 176
+ Y S L+ T TGE K CER+F +L ++ + K+ C M D
Sbjct: 377 GKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRH-RRTHTGEKKFACPMCD 435
Query: 177 R 177
R
Sbjct: 436 R 436
>PR33_DAUCA (P06600) Proline-rich 33 kDa extensin-related protein
precursor (Fragment)
Length = 211
Score = 32.7 bits (73), Expect = 0.94
Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 4/37 (10%)
Query: 56 PVHTTPTYMNPFHVS--LSPP--SPPSTFEPPLASTN 88
P+H P Y P H +PP PPS ++PP+ +TN
Sbjct: 36 PIHKPPVYTPPVHKPPVYTPPVHKPPSEYKPPVEATN 72
>SUM1_YEAST (P46676) Suppressor of mar1-1 protein (SUM1-1 protein)
Length = 1062
Score = 31.2 bits (69), Expect = 2.7
Identities = 23/99 (23%), Positives = 43/99 (43%), Gaps = 2/99 (2%)
Query: 13 LICSSISSSTKSHPFFLSQMEMKKAENVKNNINDPLALSLSVEPVHTTPTYMNPFHVSLS 72
L+ S+ SSS L+ + ++ N A S S+ P TP +
Sbjct: 272 LLLSAPSSSRDDADMSLTSVP-QRTNNENGKERPSTANSSSITPTPVTPNNLIQIKRKRG 330
Query: 73 PPSPPSTFEPPLASTNDSID-SSSSRRVRRRVEKSEIIP 110
P T E ++++ D+ID S +S R++ + + ++P
Sbjct: 331 RPPKKRTVETMISNSTDTIDKSDASNRIKNEIPINSLLP 369
>PGCA_CANFA (Q28343) Aggrecan core protein precursor
(Cartilage-specific proteoglycan core protein) (CSPCP)
Length = 2333
Score = 30.8 bits (68), Expect = 3.6
Identities = 22/69 (31%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Query: 50 LSLSVEPVHTT---PTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKS 106
L ++EP + T P Y +P S P PP T+ P +T +S + S V E+
Sbjct: 739 LDFTIEPENQTEWEPAY-SPAGTSPLPGIPP-TWPPTSTATEESTEGPSGTEVPSVSEEP 796
Query: 107 EIIPPPFPW 115
PFPW
Sbjct: 797 SPSEEPFPW 805
>CCAA_RAT (P54282) Voltage-dependent P/Q-type calcium channel alpha-1A
subunit (Voltage-gated calcium channel alpha subunit
Cav2.1) (Calcium channel, L type, alpha-1 polypeptide,
isoform 4) (Brain calcium channel I) (BI) (RAT brain
class A) (RBA-I)
Length = 2212
Score = 30.8 bits (68), Expect = 3.6
Identities = 24/76 (31%), Positives = 35/76 (45%), Gaps = 3/76 (3%)
Query: 40 VKNNINDPLALSLSVEPVHTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRV 99
+ N+ N AL+ + + + T NP + S P PP T E L TN S +S +
Sbjct: 1055 IGNSTNPGPALATNPQNAASRRTPNNPGNPS--NPGPPKTPENSLIVTNPSSTQPNSAKT 1112
Query: 100 RRRVEKSEI-IPPPFP 114
R+ E + IPP P
Sbjct: 1113 ARKPEHMAVEIPPACP 1128
>ARR4_ARATH (O82798) Two-component response regulator ARR4 (Response
regulator 1)
Length = 259
Score = 30.8 bits (68), Expect = 3.6
Identities = 24/93 (25%), Positives = 44/93 (46%), Gaps = 8/93 (8%)
Query: 8 IYSSQLICSSISSSTK--SHPFFLSQMEMKKAENVKNNINDPLALSLSVEPVHTTPTYMN 65
I SS+ + + I + + F L +++ + +++++ + LS + P +
Sbjct: 123 IMSSENVLTRIDRCLEEGAQDFLLKPVKLADVKRLRSHLTKDVKLSNGNK--RKLPEDSS 180
Query: 66 PFHVSLSPPSPPSTF----EPPLASTNDSIDSS 94
+ SL PPSPP T PPL + +S DSS
Sbjct: 181 SVNSSLPPPSPPLTISPESSPPLTVSTESSDSS 213
>AK80_RAT (O35817) Testis-specific A-kinase-anchoring-protein
TAKAP-80 (TAKAP-1.2)
Length = 502
Score = 30.8 bits (68), Expect = 3.6
Identities = 20/85 (23%), Positives = 41/85 (47%), Gaps = 6/85 (7%)
Query: 47 PLALSLSVEPV-HTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEK 105
PLA + PV HT P +P +PP++ PP+ ++ + + +V++++
Sbjct: 214 PLAPVVPPAPVAHTEPVAHAVPFAPPAPVAPPASVAPPVPDAPVALVAPVAPQVKQKIRV 273
Query: 106 SEI-----IPPPFPWATDRRARVYT 125
SE+ +PP P + + ++T
Sbjct: 274 SEVKRKKEVPPRVPQLKEMKVVLFT 298
>HXA3_HETFR (Q9IA21) Homeobox protein Hox-A3
Length = 410
Score = 30.4 bits (67), Expect = 4.7
Identities = 15/48 (31%), Positives = 25/48 (51%), Gaps = 1/48 (2%)
Query: 73 PPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPPPFPWATDRR 120
PP PP + PP ++++S SS+S+ ++ I FPW + R
Sbjct: 95 PPPPPPSVSPPQNTSSNSTQSSTSKN-PTLTSQATISKQIFPWMKESR 141
>EVI1_MOUSE (P14404) Ecotropic virus integration 1 site protein
Length = 1042
Score = 30.4 bits (67), Expect = 4.7
Identities = 15/58 (25%), Positives = 31/58 (52%), Gaps = 2/58 (3%)
Query: 122 RVYTLSHLLQNQIFTITGE--VQCKRCERKFEMGFDLKKNFSEVSSFIAKYECTMHDR 177
+++ S L + T TGE +CK C+R F + +L+++ + + ++C + DR
Sbjct: 731 KIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDR 788
>CCAA_RABIT (P27884) Voltage-dependent P/Q-type calcium channel
alpha-1A subunit (Voltage-gated calcium channel alpha
subunit Cav2.1) (Calcium channel, L type, alpha-1
polypeptide isoform 4) (Brain calcium channel I) (BI)
Length = 2424
Score = 30.4 bits (67), Expect = 4.7
Identities = 24/81 (29%), Positives = 35/81 (42%), Gaps = 13/81 (16%)
Query: 47 PLALSLSVEPVHTTP-TYMNPFHVSLSP-----------PSPPSTFEPPLASTNDSIDSS 94
P + S +P TP T NP + + S P PP T E L TN S +
Sbjct: 1105 PAKMGSSTDPAGPTPATAANPQNSTASRRTPNNPGNPSNPGPPKTPENSLIVTNPSTAQT 1164
Query: 95 SSRRVRRRVEKSEI-IPPPFP 114
+S + R+ + + + IPP P
Sbjct: 1165 NSAKTARKPDHTTVEIPPACP 1185
>CA1H_HUMAN (P39060) Collagen alpha 1(XVIII) chain precursor
[Contains: Endostatin]
Length = 1516
Score = 30.4 bits (67), Expect = 4.7
Identities = 15/45 (33%), Positives = 23/45 (50%)
Query: 52 LSVEPVHTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSS 96
L V+P T T++ P + S P + P + EPP D D+ +S
Sbjct: 52 LPVQPTADTTTHVTPRNGSTEPATAPGSPEPPSELLEDGQDTPTS 96
>YDQ2_SCHPO (O14196) Hypothetical protein C5D6.02c in chromosome I
Length = 300
Score = 30.0 bits (66), Expect = 6.1
Identities = 16/44 (36%), Positives = 22/44 (49%)
Query: 85 ASTNDSIDSSSSRRVRRRVEKSEIIPPPFPWATDRRARVYTLSH 128
+ +NDS S RR RR+V K EI+ P + + TL H
Sbjct: 67 SQSNDSAAIPSYRRKRRKVRKPEIVKPTLRKRGRKPKNISTLEH 110
>WAS1_HUMAN (Q92558) Wiskott-Aldrich syndrome protein family member
1 (WASP-family protein member 1) (Verprolin homology
domain-containing protein 1)
Length = 559
Score = 30.0 bits (66), Expect = 6.1
Identities = 22/60 (36%), Positives = 28/60 (46%), Gaps = 7/60 (11%)
Query: 62 TYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPPPFPWATDRRA 121
T P VS +PP PP PPL S ++ +SS R + PPP P AT +A
Sbjct: 313 TGRTPVFVSPTPPPPP----PPLPS---ALSTSSLRASMTSTPPPPVPPPPPPPATALQA 365
>MB11_COPCI (P40333) Mating-type protein beta1-1
Length = 681
Score = 30.0 bits (66), Expect = 6.1
Identities = 22/93 (23%), Positives = 42/93 (44%), Gaps = 12/93 (12%)
Query: 60 TPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVR----------RRVE-KSEI 108
+P+ + + + PSP + P L T DS S+ R+ R +R E + ++
Sbjct: 403 SPSTQDELLETSAAPSPQPSLLPKLTPT-DSARSTGKRKRRLSDGFQYPAAKRPEIRPQV 461
Query: 109 IPPPFPWATDRRARVYTLSHLLQNQIFTITGEV 141
+ PFP + + H+L + T+TG++
Sbjct: 462 VSDPFPATSSEHWEQWFREHVLSSPELTLTGDI 494
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.133 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,433,696
Number of Sequences: 164201
Number of extensions: 1247182
Number of successful extensions: 4418
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 32
Number of HSP's that attempted gapping in prelim test: 4387
Number of HSP's gapped (non-prelim): 59
length of query: 266
length of database: 59,974,054
effective HSP length: 108
effective length of query: 158
effective length of database: 42,240,346
effective search space: 6673974668
effective search space used: 6673974668
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0150.12