BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0145.4
(72 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KMLS_CHICK (P11799) Myosin light chain kinase, smooth muscle and... 34 0.079
MU5A_HUMAN (P98088) Mucin 5AC (Mucin 5 subtype AC, tracheobronch... 32 0.39
TOP1_ZYMMO (Q9X3X7) DNA topoisomerase I (EC 5.99.1.2) (Omega-pro... 30 1.5
YQI9_CAEEL (Q09507) Hypothetical protein C45G9.9 in chromosome III 29 1.9
YQI4_CAEEL (Q09505) Hypothetical protein C45G9.4 in chromosome III 29 1.9
MST2_DROHY (Q08696) Axoneme-associated protein mst101(2) 29 1.9
GLS2_YEAST (P40989) 1,3-beta-glucan synthase component GLS2 (EC ... 29 1.9
SOC6_HUMAN (O14544) Suppressor of cytokine signaling 6 (Suppress... 29 2.5
RADA_CERSY (O93748) DNA repair and recombination protein radA 28 3.3
NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa neur... 28 3.3
DX52_HUMAN (Q9Y2R4) DEAD-box protein 52 (EC 3.6.1.-) (Putative A... 28 3.3
ARLY_SACDO (P41906) Argininosuccinate lyase (EC 4.3.2.1) (Argino... 28 3.3
RS2_THEMA (Q9WZM1) 30S ribosomal protein S2 27 7.4
RMX2_MOUSE (Q8R0F5) RNA binding motif protein, X-linked 2 27 7.4
ISK5_HUMAN (Q9NQ38) Serine protease inhibitor Kazal-type 5 precu... 27 7.4
HS88_NEUCR (O74225) Heat shock protein Hsp88 27 7.4
EML4_HUMAN (Q9HC35) Echinoderm microtubule-associated protein-li... 27 7.4
EF1G_DROME (Q9NJH0) Elongation factor 1-gamma (EF-1-gamma) (eEF-... 27 7.4
Y539_PASMU (Q9CN96) Hypothetical UPF0260 protein PM0539 27 9.6
TTCG_MACFA (Q95LZ5) Tetratricopeptide repeat protein 16 (TPR rep... 27 9.6
>KMLS_CHICK (P11799) Myosin light chain kinase, smooth muscle and
non-muscle isozymes (EC 2.7.1.117) (MLCK) [Contains:
Telokin]
Length = 1906
Score = 33.9 bits (76), Expect = 0.079
Identities = 19/54 (35%), Positives = 24/54 (44%)
Query: 14 DFGEYKPLHSSVDAKLQAICEGLAEMQKQGKTEKKSEKPQKKSKATASKVAPQE 67
D GEYK + + K + C+ L E K K +EK KK K T V E
Sbjct: 1150 DGGEYKCIAENAAGKAECACKVLVEDTSSTKAAKPAEKKTKKPKTTLPPVLSTE 1203
>MU5A_HUMAN (P98088) Mucin 5AC (Mucin 5 subtype AC,
tracheobronchial) (Tracheobronchial mucin) (TBM) (Major
airway glycoprotein) (Fragment)
Length = 1233
Score = 31.6 bits (70), Expect = 0.39
Identities = 16/41 (39%), Positives = 22/41 (53%)
Query: 30 QAICEGLAEMQKQGKTEKKSEKPQKKSKATASKVAPQEGCC 70
+A CEG + +T + EKP + A KVA Q+GCC
Sbjct: 451 EATCEGNNVISLSPRTCPRVEKPTCANGYPAVKVADQDGCC 491
>TOP1_ZYMMO (Q9X3X7) DNA topoisomerase I (EC 5.99.1.2) (Omega-protein)
(Relaxing enzyme) (Untwisting enzyme) (Swivelase)
Length = 1217
Score = 29.6 bits (65), Expect = 1.5
Identities = 14/37 (37%), Positives = 21/37 (55%)
Query: 31 AICEGLAEMQKQGKTEKKSEKPQKKSKATASKVAPQE 67
A+ E A+M K+ KT+K + K K T K AP++
Sbjct: 1107 ALLEARAKMPKKKKTKKAAAKKPAAKKTTTKKAAPKK 1143
>YQI9_CAEEL (Q09507) Hypothetical protein C45G9.9 in chromosome
III
Length = 294
Score = 29.3 bits (64), Expect = 1.9
Identities = 17/54 (31%), Positives = 30/54 (55%)
Query: 14 DFGEYKPLHSSVDAKLQAICEGLAEMQKQGKTEKKSEKPQKKSKATASKVAPQE 67
D + KP SS +K + +GL + + K+S+K K++K +A K AP++
Sbjct: 23 DKSKTKPRRSSKTSKKRKSKKGLFGCCAKKRKTKRSKKSAKRTKRSAPKKAPKK 76
>YQI4_CAEEL (Q09505) Hypothetical protein C45G9.4 in chromosome
III
Length = 292
Score = 29.3 bits (64), Expect = 1.9
Identities = 17/54 (31%), Positives = 30/54 (55%)
Query: 14 DFGEYKPLHSSVDAKLQAICEGLAEMQKQGKTEKKSEKPQKKSKATASKVAPQE 67
D + KP SS +K + +GL + + K+S+K K++K +A K AP++
Sbjct: 23 DKSKTKPRRSSKTSKKRKSKKGLFGCCAKKRKTKRSKKSAKRTKRSAPKKAPKK 76
>MST2_DROHY (Q08696) Axoneme-associated protein mst101(2)
Length = 1391
Score = 29.3 bits (64), Expect = 1.9
Identities = 14/30 (46%), Positives = 17/30 (56%)
Query: 33 CEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
CE A+ +KQ +KK EK KK K A K
Sbjct: 861 CEKAAKKRKQAAEKKKCEKAAKKRKEAAEK 890
Score = 29.3 bits (64), Expect = 1.9
Identities = 14/30 (46%), Positives = 18/30 (59%)
Query: 33 CEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
CE A+ +K+ +KK EK KK K TA K
Sbjct: 829 CEKAAKKRKEEAEKKKCEKTAKKRKETAEK 858
Score = 28.9 bits (63), Expect = 2.5
Identities = 14/30 (46%), Positives = 19/30 (62%)
Query: 33 CEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
CE LA+ K+ + +KK +K KK K TA K
Sbjct: 495 CEELAKKIKKAEIKKKCKKLAKKEKETAEK 524
Score = 28.1 bits (61), Expect = 4.3
Identities = 13/30 (43%), Positives = 17/30 (56%)
Query: 33 CEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
CE A+ +K+ +KK EK KK K A K
Sbjct: 527 CEKAAKKRKEAAEKKKCEKAAKKRKEAAEK 556
Score = 28.1 bits (61), Expect = 4.3
Identities = 13/30 (43%), Positives = 17/30 (56%)
Query: 33 CEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
CE A+ +K+ +KK EK KK K A K
Sbjct: 543 CEKAAKKRKEAAEKKKCEKSAKKRKEAAEK 572
Score = 27.7 bits (60), Expect = 5.7
Identities = 13/30 (43%), Positives = 17/30 (56%)
Query: 33 CEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
CE A+ +K+ +KK EK KK K A K
Sbjct: 813 CEKTAKKRKEAAEKKKCEKAAKKRKEEAEK 842
Score = 27.3 bits (59), Expect = 7.4
Identities = 13/30 (43%), Positives = 17/30 (56%)
Query: 33 CEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
CE A+ +K+ +KK EK KK K A K
Sbjct: 845 CEKTAKKRKETAEKKKCEKAAKKRKQAAEK 874
Score = 27.3 bits (59), Expect = 7.4
Identities = 13/30 (43%), Positives = 17/30 (56%)
Query: 33 CEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
CE A+ +K+ +KK EK KK K A K
Sbjct: 797 CEKTAKKRKEEAEKKKCEKTAKKRKEAAEK 826
>GLS2_YEAST (P40989) 1,3-beta-glucan synthase component GLS2 (EC
2.4.1.34) (1,3-beta-D-glucan-UDP glucosyltransferase)
Length = 1895
Score = 29.3 bits (64), Expect = 1.9
Identities = 17/57 (29%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Query: 11 VGGDFGEYKPLHSSVDAKLQAICEGLAEMQKQGKTEKKSEKPQKKSKATASKVAPQE 67
+GGD YK + + A+L E K GK +K+ K +KK+K + +P++
Sbjct: 233 IGGDTANYKKWYFA--AQLDMDDEIGFRNMKLGKLSRKARKAKKKNKKAMQEASPED 287
>SOC6_HUMAN (O14544) Suppressor of cytokine signaling 6
(Suppressor of cytokine signaling 4) (SOCS-4)
(Cytokine-inducible SH2 protein 4) (CIS-4)
Length = 535
Score = 28.9 bits (63), Expect = 2.5
Identities = 17/44 (38%), Positives = 21/44 (47%)
Query: 7 QGSSVGGDFGEYKPLHSSVDAKLQAICEGLAEMQKQGKTEKKSE 50
Q S+ DFG+ L S K A C+ E +K GK KSE
Sbjct: 28 QQPSLASDFGKDDSLFGSCYGKDMASCDINGEDEKGGKNRSKSE 71
>RADA_CERSY (O93748) DNA repair and recombination protein radA
Length = 369
Score = 28.5 bits (62), Expect = 3.3
Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Query: 34 EGLAEMQKQGK-TEKKSEKPQKKSKATASKVAPQ 66
E L ++ K+ K EK++ KP KKSKA A P+
Sbjct: 294 EHLKQLDKEAKKAEKEAAKPVKKSKAKAKADGPE 327
>NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa
neurofilament protein) (Neurofilament medium
polypeptide) (NF-M) (Neurofilament 3)
Length = 915
Score = 28.5 bits (62), Expect = 3.3
Identities = 16/46 (34%), Positives = 26/46 (55%), Gaps = 3/46 (6%)
Query: 23 SSVDAKLQAICEGLAEMQKQGK---TEKKSEKPQKKSKATASKVAP 65
S ++ L AI E LA K+ K E+K E+P+ + + A+K +P
Sbjct: 466 SEMEEALTAITEELAASMKEEKKEAAEEKEEEPEAEEEEVAAKKSP 511
>DX52_HUMAN (Q9Y2R4) DEAD-box protein 52 (EC 3.6.1.-) (Putative
ATP-dependent RNA helicase ROK1-like) (HUSSY-19)
Length = 599
Score = 28.5 bits (62), Expect = 3.3
Identities = 12/30 (40%), Positives = 20/30 (66%)
Query: 38 EMQKQGKTEKKSEKPQKKSKATASKVAPQE 67
E +++ TE+K E+ +KK K S++A QE
Sbjct: 72 EKKEESLTERKREQSKKKRKTMTSEIASQE 101
>ARLY_SACDO (P41906) Argininosuccinate lyase (EC 4.3.2.1)
(Arginosuccinase) (ASAL)
Length = 463
Score = 28.5 bits (62), Expect = 3.3
Identities = 15/37 (40%), Positives = 22/37 (58%)
Query: 26 DAKLQAICEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
DA+L I EGLAE+QK+ +K P + TA++
Sbjct: 57 DAELAKIHEGLAEIQKEWDADKFVRHPNDEDIHTANE 93
>RS2_THEMA (Q9WZM1) 30S ribosomal protein S2
Length = 262
Score = 27.3 bits (59), Expect = 7.4
Identities = 15/58 (25%), Positives = 28/58 (47%), Gaps = 5/58 (8%)
Query: 11 VGGDFGEYKPLHSSVDAKLQAICEGLAEMQKQGKTEKKSEKPQKKSKATASKVAPQEG 68
+GG +K + S +D ++ L EM++ GK E+ +K Q + + K+ G
Sbjct: 97 LGGLLTNFKTIRSRIDKLIE-----LEEMEQSGKLEELPKKEQSRIRRVLEKLRKNLG 149
>RMX2_MOUSE (Q8R0F5) RNA binding motif protein, X-linked 2
Length = 326
Score = 27.3 bits (59), Expect = 7.4
Identities = 12/33 (36%), Positives = 18/33 (54%)
Query: 37 AEMQKQGKTEKKSEKPQKKSKATASKVAPQEGC 69
A++ K+ K +KK +K +KK K A Q C
Sbjct: 154 AKLTKKHKKDKKEKKKRKKEKTEGQAQAEQPSC 186
>ISK5_HUMAN (Q9NQ38) Serine protease inhibitor Kazal-type 5
precursor (Lympho-epithelial Kazal-type related
inhibitor) (LEKTI) [Contains: Hemofiltrate peptide
HF6478; Hemofiltrate peptide HF7665]
Length = 1064
Score = 27.3 bits (59), Expect = 7.4
Identities = 15/47 (31%), Positives = 23/47 (48%)
Query: 16 GEYKPLHSSVDAKLQAICEGLAEMQKQGKTEKKSEKPQKKSKATASK 62
G Y +H + A Q+I + A +K+ EK S KP +K S+
Sbjct: 872 GPYGKMHINKCAMCQSIFDREANERKKKDEEKSSSKPSNNAKDECSE 918
>HS88_NEUCR (O74225) Heat shock protein Hsp88
Length = 707
Score = 27.3 bits (59), Expect = 7.4
Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Query: 28 KLQAICEGLAEMQKQGKTEKKSEKPQKKSKATASKVAPQEG 68
K++A + L E + K KK+E+ +K+K A K A QEG
Sbjct: 649 KVEAERQALQEKLEAEKAAKKAEEEARKAKEAAEKAA-QEG 688
>EML4_HUMAN (Q9HC35) Echinoderm microtubule-associated protein-like
4 (EMAP-4) (Restrictedly overexpressed
proliferation-associated protein) (Ropp 120)
Length = 981
Score = 27.3 bits (59), Expect = 7.4
Identities = 23/58 (39%), Positives = 30/58 (51%), Gaps = 7/58 (12%)
Query: 13 GDFGEYKPLHSSVDAKLQAICEGLAEMQKQGKTEKKSEKPQ----KKSKATASKVAPQ 66
G KP H+S A A E L+ K G TEKK EKPQ KK ++ ++ +PQ
Sbjct: 86 GSGANRKPSHTS--AVSIAGKETLSSAAKSG-TEKKKEKPQGQREKKEESHSNDQSPQ 140
>EF1G_DROME (Q9NJH0) Elongation factor 1-gamma (EF-1-gamma) (eEF-1B
gamma)
Length = 431
Score = 27.3 bits (59), Expect = 7.4
Identities = 11/33 (33%), Positives = 20/33 (60%)
Query: 35 GLAEMQKQGKTEKKSEKPQKKSKATASKVAPQE 67
G A+ Q+Q + +K+ +KP++K +A P E
Sbjct: 225 GAAKPQQQAQQQKQEKKPKEKKEAPKKAAEPAE 257
>Y539_PASMU (Q9CN96) Hypothetical UPF0260 protein PM0539
Length = 148
Score = 26.9 bits (58), Expect = 9.6
Identities = 11/27 (40%), Positives = 18/27 (65%)
Query: 9 SSVGGDFGEYKPLHSSVDAKLQAICEG 35
SS+ DF ++K L DA+ +A+C+G
Sbjct: 3 SSIEPDFWKHKTLLEMTDAEWEALCDG 29
>TTCG_MACFA (Q95LZ5) Tetratricopeptide repeat protein 16 (TPR repeat
protein 16) (QtsA-13222)
Length = 872
Score = 26.9 bits (58), Expect = 9.6
Identities = 15/28 (53%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
Query: 38 EMQKQGKTEKKSEKPQ-KKSKATASKVA 64
E QK+ + +KK EK + KK K T SKVA
Sbjct: 585 EEQKEEEEQKKEEKKEEKKPKLTPSKVA 612
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.131 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,929,248
Number of Sequences: 164201
Number of extensions: 246678
Number of successful extensions: 1525
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1478
Number of HSP's gapped (non-prelim): 53
length of query: 72
length of database: 59,974,054
effective HSP length: 48
effective length of query: 24
effective length of database: 52,092,406
effective search space: 1250217744
effective search space used: 1250217744
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0145.4