BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0140.2
(123 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HCRA_THAAR (O33819) 4-hydroxybenzoyl-CoA reductase alpha subunit... 32 0.19
STUA_EMENI (P36011) Cell pattern formation-associated protein stuA 30 0.73
HNFA_RAT (P15257) Hepatocyte nuclear factor 1-alpha (HNF-1A) (Li... 29 1.6
ZIC4_HUMAN (Q8N9L1) Zinc finger protein ZIC 4 (Zinc finger prote... 29 2.1
PIA4_MOUSE (Q9JM05) Protein inhibitor of activated STAT protein ... 29 2.1
HNFA_MOUSE (P22361) Hepatocyte nuclear factor 1-alpha (HNF-1A) (... 29 2.1
YIS3_YEAST (P40563) Hypothetical 74.8 kDa protein in BET1-PAN1 i... 28 3.6
UL50_HCMVA (P16791) Protein UL50 28 3.6
OSTA_PASMU (Q9CKL2) Organic solvent tolerance protein precursor 28 3.6
PURA_COXBU (Q83CV4) Adenylosuccinate synthetase (EC 6.3.4.4) (IM... 28 4.7
S3A2_MOUSE (Q62203) Splicing factor 3A subunit 2 (Spliceosome as... 27 6.2
LA17_YEAST (Q12446) Proline-rich protein LAS17 27 6.2
HFA1_YEAST (P32874) HFA1 protein 27 6.2
DRPL_RAT (P54258) Atrophin-1 (Dentatorubral-pallidoluysian atrop... 27 6.2
BRO_DROME (Q24039) Brother protein 27 8.1
B3AR_MACMU (Q28524) Beta-3 adrenergic receptor (Beta-3 adrenocep... 27 8.1
>HCRA_THAAR (O33819) 4-hydroxybenzoyl-CoA reductase alpha subunit
(EC 1.3.99.20)
Length = 769
Score = 32.3 bits (72), Expect = 0.19
Identities = 23/77 (29%), Positives = 32/77 (40%), Gaps = 5/77 (6%)
Query: 45 NLMPRTPCVIMIASTPPSQHHHSCKPTVNRAPGWFPEKRGVFPEPPKTALHNVTLLQHLR 104
N++P+ P V M A S C V A GW E++G P+ + L H
Sbjct: 391 NMLPQIPYVTMYAQRVMSYGVPECLEKVKAASGW-EERKGKLPKGRGLGI----ALSHFV 445
Query: 105 NTTQSDIHRTGSSDTTV 121
+ T + H TG TV
Sbjct: 446 SGTSTPKHWTGEPHATV 462
>STUA_EMENI (P36011) Cell pattern formation-associated protein stuA
Length = 622
Score = 30.4 bits (67), Expect = 0.73
Identities = 17/42 (40%), Positives = 22/42 (51%), Gaps = 5/42 (11%)
Query: 57 ASTPPSQHHHSCK---PTVNRAPGWFP--EKRGVFPEPPKTA 93
A PPS HHHS + P+ PG P ++ FP PP +A
Sbjct: 263 AQQPPSLHHHSLQTPVPSHMSQPGGRPSLDRAHTFPTPPASA 304
>HNFA_RAT (P15257) Hepatocyte nuclear factor 1-alpha (HNF-1A)
(Liver-specific transcription factor LF-B1) (LFB1)
Length = 628
Score = 29.3 bits (64), Expect = 1.6
Identities = 27/112 (24%), Positives = 50/112 (44%), Gaps = 16/112 (14%)
Query: 20 MSTNARTLSPHILHLYQPMLVCHL-DNLMPRTPCVI-----MIASTPPSQHHHSCKPTVN 73
M+T A+ SPH L+ ++P + + +L+P+T + +AS P++ + +
Sbjct: 489 MATMAQLQSPHALYSHKPEVAQYTHTSLLPQTMLITDTNLSTLASLTPTKQVFTSDTEAS 548
Query: 74 RAPGWFPEKRGVFPEPPKTALH----NVTLLQHLRNTTQSDIHRTGSSDTTV 121
PG P P T +H + + +QHL+ + T SS + V
Sbjct: 549 SEPGLHE------PSSPATTIHIPSQDPSNIQHLQPAHRLSTSPTVSSSSLV 594
>ZIC4_HUMAN (Q8N9L1) Zinc finger protein ZIC 4 (Zinc finger
protein of the cerebellum 4) (Fragment)
Length = 320
Score = 28.9 bits (63), Expect = 2.1
Identities = 13/42 (30%), Positives = 22/42 (51%), Gaps = 3/42 (7%)
Query: 52 CVIMIASTPPSQHHHSCKPTVNRAPGWFPEKRGVFPEPPKTA 93
C + +++T S HH + T +P FP G+ EPP+ +
Sbjct: 2 CALFVSTTGSSSGHHGPQLTAASSPSVFP---GLHEEPPQAS 40
>PIA4_MOUSE (Q9JM05) Protein inhibitor of activated STAT protein 4
(Protein inhibitor of activated STAT protein gamma)
(PIAS-gamma) (PIASy)
Length = 507
Score = 28.9 bits (63), Expect = 2.1
Identities = 26/100 (26%), Positives = 43/100 (43%), Gaps = 17/100 (17%)
Query: 13 ITTYLASMSTNARTLSPHILHLYQPMLVCHLDNLMPRTPCVIMIASTPPS----QHHHSC 68
+T M N+R L P + + + +C+ D P+ PP+ +H C
Sbjct: 161 LTPRQVEMIRNSRELQPGVKAVQVVLRICYSDTSCPQED------QYPPNIAVKVNHSYC 214
Query: 69 KPTVNRAPGWFP-EKRGVFPEPPKTALHNVTLLQHLRNTT 107
PG++P K GV P+ P + N+T L +L + T
Sbjct: 215 S-----VPGYYPSNKPGVEPKRPCRPI-NLTHLMYLSSAT 248
>HNFA_MOUSE (P22361) Hepatocyte nuclear factor 1-alpha (HNF-1A)
(Liver-specific transcription factor LF-B1) (LFB1)
Length = 628
Score = 28.9 bits (63), Expect = 2.1
Identities = 27/112 (24%), Positives = 50/112 (44%), Gaps = 16/112 (14%)
Query: 20 MSTNARTLSPHILHLYQPMLVCHL-DNLMPRTPCVI-----MIASTPPSQHHHSCKPTVN 73
M+T A+ SPH L+ ++P + + +L+P+T + +AS P++ + +
Sbjct: 489 MATMAQLQSPHALYSHKPEVAQYTHTSLLPQTMLITDTNLSTLASLTPTKQVFTSDTEAS 548
Query: 74 RAPGWFPEKRGVFPEPPKTALH----NVTLLQHLRNTTQSDIHRTGSSDTTV 121
PG P P T +H + + +QHL+ + T SS + V
Sbjct: 549 SEPGLHE------PPSPATTIHIPSQDPSNIQHLQPAHRLSTSPTVSSSSLV 594
>YIS3_YEAST (P40563) Hypothetical 74.8 kDa protein in BET1-PAN1
intergenic region
Length = 679
Score = 28.1 bits (61), Expect = 3.6
Identities = 13/49 (26%), Positives = 22/49 (44%)
Query: 72 VNRAPGWFPEKRGVFPEPPKTALHNVTLLQHLRNTTQSDIHRTGSSDTT 120
+ + P P++R P P K + + L+ Q D+H G+S T
Sbjct: 376 IPKIPSERPKRRAPPPVPKKPSSRIAAFQEMLQKQQQQDLHNNGNSSAT 424
>UL50_HCMVA (P16791) Protein UL50
Length = 397
Score = 28.1 bits (61), Expect = 3.6
Identities = 16/37 (43%), Positives = 17/37 (45%), Gaps = 3/37 (8%)
Query: 57 ASTPPSQHHHSCKPTVNRAPGWFPEKRGVFPEPPKTA 93
AS PP H SC PT+ A G G P PP A
Sbjct: 215 ASPPPPPRHPSCSPTMVAAGG---AAAGPRPPPPPMA 248
>OSTA_PASMU (Q9CKL2) Organic solvent tolerance protein precursor
Length = 782
Score = 28.1 bits (61), Expect = 3.6
Identities = 13/31 (41%), Positives = 19/31 (60%), Gaps = 4/31 (12%)
Query: 11 IDITTYLASMST----NARTLSPHILHLYQP 37
+D+ T LAS T +TL PH+ +LY+P
Sbjct: 477 VDLQTVLASQQTFIEGYTQTLEPHVQYLYRP 507
>PURA_COXBU (Q83CV4) Adenylosuccinate synthetase (EC 6.3.4.4)
(IMP--aspartate ligase) (AdSS) (AMPSase)
Length = 435
Score = 27.7 bits (60), Expect = 4.7
Identities = 15/52 (28%), Positives = 24/52 (45%), Gaps = 4/52 (7%)
Query: 56 IASTPPSQHH--HSCKPTVNRAPGWFPEKRGV--FPEPPKTALHNVTLLQHL 103
+ + PP SC+P PGW G+ + E PK A + ++ L+ L
Sbjct: 352 VVNEPPFDQSLLESCEPVYEEMPGWQTSTYGLTDYSEMPKEARNYISRLEEL 403
>S3A2_MOUSE (Q62203) Splicing factor 3A subunit 2 (Spliceosome
associated protein 62) (SAP 62) (SF3a66)
Length = 475
Score = 27.3 bits (59), Expect = 6.2
Identities = 14/32 (43%), Positives = 18/32 (55%), Gaps = 1/32 (3%)
Query: 60 PPSQHHHSCKPTVNR-APGWFPEKRGVFPEPP 90
PP+ H P V+ APG P+ GV P+PP
Sbjct: 392 PPAPAVHPQAPGVHPPAPGIHPQAPGVHPQPP 423
>LA17_YEAST (Q12446) Proline-rich protein LAS17
Length = 633
Score = 27.3 bits (59), Expect = 6.2
Identities = 20/59 (33%), Positives = 27/59 (44%), Gaps = 8/59 (13%)
Query: 60 PPSQHHHSCKPTVNRAPG--WFPE---KRGVFPEPPKTALH---NVTLLQHLRNTTQSD 110
PP H H T+N A G P+ +RG P PP A NVT+ Q+ + S+
Sbjct: 355 PPPPHRHVTSNTLNSAGGNSLLPQATGRRGPAPPPPPRASRPTPNVTMQQNPQQYNNSN 413
>HFA1_YEAST (P32874) HFA1 protein
Length = 2273
Score = 27.3 bits (59), Expect = 6.2
Identities = 14/31 (45%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Query: 83 RGVFPEPPKTALHNVTLLQ-HLRNTTQSDIH 112
RG+FP K H TLLQ H+++T +IH
Sbjct: 1094 RGIFPPIKKRKEHIKTLLQLHIKDTGAENIH 1124
>DRPL_RAT (P54258) Atrophin-1 (Dentatorubral-pallidoluysian atrophy
protein)
Length = 1183
Score = 27.3 bits (59), Expect = 6.2
Identities = 30/121 (24%), Positives = 41/121 (33%), Gaps = 8/121 (6%)
Query: 5 SGSSSAIDITTYLASMSTNARTLSPHILHLYQPMLVCHLDNLMPR-------TPCVIMIA 57
S SS A ++ A+ A P H + P + N P+ + V
Sbjct: 380 SSSSVAASSSSSAATSQYPASQTLPSYPHSFPPPTSMSVSNQPPKYTQPSLPSQAVWSQG 439
Query: 58 STPPSQHHHSCKPTVNRAPGWFPEKRG-VFPEPPKTALHNVTLLQHLRNTTQSDIHRTGS 116
PP + P N PG FP G PP A H+ Q + Q H G+
Sbjct: 440 PPPPPPPYGRLLPNNNTHPGPFPPTGGQSTAHPPAPAHHHHQQQQQPQPQPQPQQHHHGN 499
Query: 117 S 117
S
Sbjct: 500 S 500
>BRO_DROME (Q24039) Brother protein
Length = 213
Score = 26.9 bits (58), Expect = 8.1
Identities = 11/21 (52%), Positives = 12/21 (56%), Gaps = 1/21 (4%)
Query: 59 TPPSQHHHSCKPTVNRAP-GW 78
TPP HHH P + R P GW
Sbjct: 193 TPPEDHHHRGGPGLPRGPMGW 213
>B3AR_MACMU (Q28524) Beta-3 adrenergic receptor (Beta-3
adrenoceptor) (Beta-3 adrenoreceptor)
Length = 418
Score = 26.9 bits (58), Expect = 8.1
Identities = 16/51 (31%), Positives = 22/51 (42%), Gaps = 4/51 (7%)
Query: 39 LVCHLDNLMPRTPCVIMIASTPPSQHHHSCKP-TVNRAPGWFPEKRGVFPE 88
L+CH +PR PC A P S +P R+PG+ G+ E
Sbjct: 359 LLCHCGGRLPREPCA---ADRPASSPRAPLRPGPAPRSPGFASGSTGLLGE 406
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.131 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,402,756
Number of Sequences: 164201
Number of extensions: 568245
Number of successful extensions: 1152
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 1149
Number of HSP's gapped (non-prelim): 18
length of query: 123
length of database: 59,974,054
effective HSP length: 99
effective length of query: 24
effective length of database: 43,718,155
effective search space: 1049235720
effective search space used: 1049235720
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0140.2