BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0140.12
(414 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
S127_MOUSE (Q9WVL3) Solute carrier family 12 member 7 (Electrone... 293 4e-79
S125_HUMAN (Q9H2X9) Solute carrier family 12 member 5 (Electrone... 272 1e-72
S125_RAT (Q63633) Solute carrier family 12 member 5 (Electroneut... 271 2e-72
S125_MOUSE (Q91V14) Solute carrier family 12 member 5 (Electrone... 271 2e-72
S122_MOUSE (P55012) Solute carrier family 12 member 2 (Bumetanid... 220 5e-57
S122_SQUAC (P55013) Solute carrier family 12 member 2 (Bumetanid... 216 7e-56
S122_HUMAN (P55011) Solute carrier family 12 member 2 (Bumetanid... 216 7e-56
S124_HUMAN (Q9UP95) Solute carrier family 12 member 4 (Electrone... 209 1e-53
S124_RAT (Q63632) Solute carrier family 12 member 4 (Electroneut... 207 3e-53
S124_MOUSE (Q9JIS8) Solute carrier family 12 member 4 (Electrone... 207 3e-53
S126_MOUSE (Q924N4) Solute carrier family 12 member 6 (Electrone... 207 4e-53
S126_HUMAN (Q9UHW9) Solute carrier family 12 member 6 (Electrone... 207 4e-53
S124_RABIT (Q28677) Solute carrier family 12 member 4 (Electrone... 207 4e-53
S127_HUMAN (Q9Y666) Solute carrier family 12 member 7 (Electrone... 201 3e-51
S121_HUMAN (Q13621) Solute carrier family 12 member 1 (Bumetanid... 201 4e-51
S123_PSEAM (P55019) Solute carrier family 12 member 3 (Thiazide-... 197 4e-50
S121_RABIT (P55015) Solute carrier family 12 member 1 (Bumetanid... 196 1e-49
NKCL_MANSE (Q25479) Bumetanide-sensitive sodium-(Potassium)-chlo... 189 9e-48
YB85_YEAST (P38329) Hypothetical 124.0 kDa protein in PBP2-ABD1 ... 178 3e-44
YRD3_CAEEL (Q09573) Hypothetical protein K02A2.3 in chromosome II 145 2e-34
>S127_MOUSE (Q9WVL3) Solute carrier family 12 member 7
(Electroneutral potassium-chloride cotransporter 4)
(K-Cl cotransporter 4)
Length = 1083
Score = 293 bits (751), Expect = 4e-79
Identities = 180/483 (37%), Positives = 257/483 (52%), Gaps = 79/483 (16%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
IVG G+ + L+VA+C TCT LTAIS+SAIATNG + GG YY+I R+LGPE G ++GL
Sbjct: 144 IVGAAGVMESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGL 203
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFRETITQVNGTTIAQPIESPSSHDLQIYG 129
CF+LG AGA+Y+LG +E FL + P+A IF+ + + S L +
Sbjct: 204 CFYLGTTFAGAMYILGTIEIFLTYISPSAAIFQAETADGEAAALLNNMRVYGSCALALMA 263
Query: 130 IVVTIVLCFI-----------------VFGGV------------------KMINR----- 149
+VV + + ++ ++ GV + NR
Sbjct: 264 VVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKTAFAPPDIPVCLLGNRTLANRNFDTC 323
Query: 150 -----VAPAFLIPVLFSLICIYLGILLAREDHPAE-------GITGLSLETLKDNWGSEY 197
V+ + L+ L C + +++ A+ GI G++ DN S Y
Sbjct: 324 AKMQVVSNGTVTTALWRLFCNGSSLGATCDEYFAQNNVTEIQGIPGVASGVFLDNLWSTY 383
Query: 198 ------------------QKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSS 239
+++ G+P + F LVG++FP+VTGIMAGSNRS
Sbjct: 384 SDKGAFVEKKGVSSVPVSEESRPGGLPYVLTDIMTYFTMLVGIYFPSVTGIMAGSNRSGD 443
Query: 240 LKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL---LTDRLLTATVAWPF 292
LKD Q+SIP GT+ A + T+F+YL +++FGA + R+K L L+ +AWP
Sbjct: 444 LKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPS 503
Query: 293 PSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFKVADGS-EPHVATLFTAF 351
P +I IG ST GA LQSLTGAPRLL AIA D I+P L+ F + EP A L TA
Sbjct: 504 PWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLQVFGHGKANGEPTWALLLTAL 563
Query: 352 LCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLVGA 411
+C ++I +LD + P ++MFFL+CY VNL+C + LL P+WRPR+KF+HW+LS +G
Sbjct: 564 ICETGILIASLDSVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGM 623
Query: 412 LLC 414
LC
Sbjct: 624 SLC 626
>S125_HUMAN (Q9H2X9) Solute carrier family 12 member 5
(Electroneutral potassium-chloride cotransporter 2)
(Erythroid K-Cl cotransporter 2) (Neuronal K-Cl
cotransporter) (hKCC2)
Length = 1116
Score = 272 bits (695), Expect = 1e-72
Identities = 174/486 (35%), Positives = 256/486 (51%), Gaps = 84/486 (17%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG+ GI + +V +C +CT LTAIS+SAIATNG + GG YY+I R+LGPE G ++GL
Sbjct: 123 VVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGL 182
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFR------ETITQVNG------------- 110
CF+LG AGA+Y+LG +E L + PA IF+ E +N
Sbjct: 183 CFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMA 242
Query: 111 TTIAQPIESPSSHDLQIYGIVVTIVLCFIVFGGVKMINRVAPAFLIPVL----------- 159
T + ++ + L G V+ +L ++ GV P F I +L
Sbjct: 243 TVVFVGVKYVNKFALVFLGCVILSILA--IYAGVIKSAFDPPNFPICLLGNRTLSRHGFD 300
Query: 160 -----------------FSLICIYLGILLAREDH-------PAEGITGLSLETLKDNWGS 195
+ L C + +++ +GI G + +K+N S
Sbjct: 301 VCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWS 360
Query: 196 EY-------QKTNDAGIPEPDGS------------VSWNFNALVGLFFPAVTGIMAGSNR 236
Y +++ + DG+ ++ F LVG++FP+VTGIMAGSNR
Sbjct: 361 SYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNR 420
Query: 237 SSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL---LTDRLLTATVA 289
S L+D Q+SIP GT+ A T+ +Y+ SV++FGA + R+K + L+ T+A
Sbjct: 421 SGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLA 480
Query: 290 WPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFKVADGS-EPHVATLF 348
WP P +I IG ST GA LQSLTGAPRLL AI+ D I+P L+ F + EP A L
Sbjct: 481 WPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLL 540
Query: 349 TAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSL 408
TA +C ++I +LD + P ++MFFL+CY VNL+C + LL P+WRPR++++HW+LS
Sbjct: 541 TACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSF 600
Query: 409 VGALLC 414
+G LC
Sbjct: 601 LGMSLC 606
>S125_RAT (Q63633) Solute carrier family 12 member 5 (Electroneutral
potassium-chloride cotransporter 2) (K-Cl cotransporter
2) (Neuronal K-Cl cotransporter) (Furosemide-sensitive
K-Cl cotransporter) (rKCC2)
Length = 1116
Score = 271 bits (693), Expect = 2e-72
Identities = 180/486 (37%), Positives = 257/486 (52%), Gaps = 84/486 (17%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG+ GI + +V +C +CT LTAIS+SAIATNG + GG YY+I R+LGPE G ++GL
Sbjct: 123 VVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGL 182
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFR------ETITQVNG------------- 110
CF+LG AGA+Y+LG +E L + PA IF+ E +N
Sbjct: 183 CFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMA 242
Query: 111 TTIAQPIESPSSHDLQIYGIVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSL-------- 162
T + ++ + L G V+ +L ++ GV P F I +L +
Sbjct: 243 TVVFVGVKYVNKFALVFLGCVILSILA--IYAGVIKSAFDPPNFPICLLGNRTLSRHGFD 300
Query: 163 ICIYLG-------------------ILLAREDH--------PAEGITGLSLETLKDN-WG 194
+C L +L A D +GI G + +K+N W
Sbjct: 301 VCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWS 360
Query: 195 SEYQK---TNDAGIPE---PDGS------------VSWNFNALVGLFFPAVTGIMAGSNR 236
S K G+P DG+ ++ F LVG++FP+VTGIMAGSNR
Sbjct: 361 SYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNR 420
Query: 237 SSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL---LTDRLLTATVA 289
S L+D Q+SIP GT+ A T+ +Y+ SV++FGA + R+K + L+ T+A
Sbjct: 421 SGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLA 480
Query: 290 WPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFKVADGS-EPHVATLF 348
WP P +I IG ST GA LQSLTGAPRLL AI+ D I+P L+ F + EP A L
Sbjct: 481 WPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLL 540
Query: 349 TAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSL 408
TA +C ++I +LD + P ++MFFL+CY VNL+C + LL P+WRPR++++HW+LS
Sbjct: 541 TACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSF 600
Query: 409 VGALLC 414
+G LC
Sbjct: 601 LGMSLC 606
>S125_MOUSE (Q91V14) Solute carrier family 12 member 5
(Electroneutral potassium-chloride cotransporter 2)
(K-Cl cotransporter 2) (Neuronal K-Cl cotransporter)
(mKCC2)
Length = 1115
Score = 271 bits (693), Expect = 2e-72
Identities = 180/486 (37%), Positives = 257/486 (52%), Gaps = 84/486 (17%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG+ GI + +V +C +CT LTAIS+SAIATNG + GG YY+I R+LGPE G ++GL
Sbjct: 123 VVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGL 182
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFR------ETITQVNG------------- 110
CF+LG AGA+Y+LG +E L + PA IF+ E +N
Sbjct: 183 CFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMA 242
Query: 111 TTIAQPIESPSSHDLQIYGIVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSL-------- 162
T + ++ + L G V+ +L ++ GV P F I +L +
Sbjct: 243 TVVFVGVKYVNKFALVFLGCVILSILA--IYAGVIKSAFDPPNFPICLLGNRTLSRHGFD 300
Query: 163 ICIYLG-------------------ILLAREDH--------PAEGITGLSLETLKDN-WG 194
+C L +L A D +GI G + +K+N W
Sbjct: 301 VCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWS 360
Query: 195 SEYQK---TNDAGIPE---PDGS------------VSWNFNALVGLFFPAVTGIMAGSNR 236
S K G+P DG+ ++ F LVG++FP+VTGIMAGSNR
Sbjct: 361 SYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNR 420
Query: 237 SSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL---LTDRLLTATVA 289
S L+D Q+SIP GT+ A T+ +Y+ SV++FGA + R+K + L+ T+A
Sbjct: 421 SGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLA 480
Query: 290 WPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFKVADGS-EPHVATLF 348
WP P +I IG ST GA LQSLTGAPRLL AI+ D I+P L+ F + EP A L
Sbjct: 481 WPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLL 540
Query: 349 TAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSL 408
TA +C ++I +LD + P ++MFFL+CY VNL+C + LL P+WRPR++++HW+LS
Sbjct: 541 TACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSF 600
Query: 409 VGALLC 414
+G LC
Sbjct: 601 LGMSLC 606
>S122_MOUSE (P55012) Solute carrier family 12 member 2
(Bumetanide-sensitive sodium-(potassium)-chloride
cotransporter 1) (Basolateral Na-K-Cl symporter)
Length = 1205
Score = 220 bits (561), Expect = 5e-57
Identities = 139/442 (31%), Positives = 219/442 (49%), Gaps = 67/442 (15%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
IVG GIG +++++A+ T +T +S SAIATNG ++GGG YYLI R+LGPE G +IGL
Sbjct: 304 IVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGL 363
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYGI 130
F NAVA A+YV+G ET ++ + I I ++N D++I G
Sbjct: 364 IFAFANAVAVAMYVVGFAETVVELLKEHSIL--MIDEIN--------------DIRIIGA 407
Query: 131 VVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETLK 190
+ ++L I G++ + L+ +L ++ +G ++ E +G G E
Sbjct: 408 ITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFN 467
Query: 191 DNWGSEYQKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSIPLG 250
+N+G ++++ F ++ +FFPA TGI+AG+N S L D Q +IP G
Sbjct: 468 ENFGPDFREEE-------------TFFSVFAIFFPAATGILAGANISGDLADPQSAIPKG 514
Query: 251 TLAATLVTTFMYLVSVIMFGALATRE------KLLTDRLLTATVA--------------- 289
TL A L+TT +Y+ + G+ R+ +T L T A
Sbjct: 515 TLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNT 574
Query: 290 ---------------WPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYF 334
F LI GI +T+ +AL SL AP++ A+ D+I P + F
Sbjct: 575 CSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMF 634
Query: 335 KVADG--SEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDA 392
G +EP + T + G ++I L++I P ++ FFL YA +N S F L +
Sbjct: 635 AKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKS 694
Query: 393 PSWRPRWKFHHWSLSLVGALLC 414
P WRP +K+++ +SL+GA+LC
Sbjct: 695 PGWRPAFKYYNMWISLIGAILC 716
>S122_SQUAC (P55013) Solute carrier family 12 member 2
(Bumetanide-sensitive sodium-(potassium)-chloride
cotransporter 1) (NA-K-CL symporter) (NKCC)
Length = 1191
Score = 216 bits (551), Expect = 7e-56
Identities = 146/443 (32%), Positives = 216/443 (47%), Gaps = 68/443 (15%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
IVG GIG LL++ T +T +S SAI TNG ++GGG YYLI R+LGPE G +IGL
Sbjct: 283 IVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGL 342
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYGI 130
F NAVA A+YV+G ET R+ + + N I + D++I G
Sbjct: 343 IFAFANAVAVAMYVVGFAET----------VRDLLVEHNALMIDE------MSDIRIIGS 386
Query: 131 VVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETLK 190
V +VL I G++ + L +L +++ +G + D A+G E
Sbjct: 387 VTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPANDKRAKGFFNYRGEIFS 446
Query: 191 DNWGSEYQKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSIPLG 250
+N+ +++ D F ++ +FFPA TGI+AG+N S L D Q +IP G
Sbjct: 447 ENFVPDFRDGED-------------FFSVFAIFFPAATGILAGANISGDLADPQLAIPKG 493
Query: 251 TLAATLVTTFMYLVSVIMFGALATREKL--LTDRLLTATV-------------------- 288
TL A L+TT +Y + + G+ RE LTD ++ TV
Sbjct: 494 TLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATN 553
Query: 289 ---------------AWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKY 333
F LI GI +T+ +AL SL AP++ A+ D+I P L
Sbjct: 554 KCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHV 613
Query: 334 FKVADG--SEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLD 391
F V G +EP + T F+ G ++I L++I P ++ FFL YA +N S F L
Sbjct: 614 FSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAK 673
Query: 392 APSWRPRWKFHHWSLSLVGALLC 414
+P WRP ++F++ +SL+GA+LC
Sbjct: 674 SPGWRPAFRFYNMWISLIGAILC 696
>S122_HUMAN (P55011) Solute carrier family 12 member 2
(Bumetanide-sensitive sodium-(potassium)-chloride
cotransporter 1) (Basolateral Na-K-Cl symporter)
Length = 1212
Score = 216 bits (551), Expect = 7e-56
Identities = 137/442 (30%), Positives = 219/442 (48%), Gaps = 67/442 (15%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
IVG GIG ++L++ + T +T +S SAIATNG ++GGG YYLI R+LGPE G +IGL
Sbjct: 311 IVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGL 370
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYGI 130
F NAVA A+YV+G ET ++ + I I ++N D++I G
Sbjct: 371 IFAFANAVAVAMYVVGFAETVVELLKEHSIL--MIDEIN--------------DIRIIGA 414
Query: 131 VVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETLK 190
+ ++L I G++ + L+ +L ++ +G + E +G G E
Sbjct: 415 ITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFN 474
Query: 191 DNWGSEYQKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSIPLG 250
+N+G ++++ F ++ +FFPA TGI+AG+N S L D Q +IP G
Sbjct: 475 ENFGPDFREEE-------------TFFSVFAIFFPAATGILAGANISGDLADPQSAIPKG 521
Query: 251 TLAATLVTTFMYLVSVIMFGALATREKL--LTDRLLT----------------------- 285
TL A L+TT +Y+ + G+ R+ + D ++T
Sbjct: 522 TLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSP 581
Query: 286 -----------ATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYF 334
++ F LI GI +T+ +AL SL AP++ A+ D+I P + F
Sbjct: 582 CSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMF 641
Query: 335 KVADG--SEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDA 392
G +EP + T + G ++I L++I P ++ FFL YA +N S F L +
Sbjct: 642 AKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKS 701
Query: 393 PSWRPRWKFHHWSLSLVGALLC 414
P WRP +K+++ +SL+GA+LC
Sbjct: 702 PGWRPAFKYYNMWISLLGAILC 723
>S124_HUMAN (Q9UP95) Solute carrier family 12 member 4
(Electroneutral potassium-chloride cotransporter 1)
(Erythroid K-Cl cotransporter 1) (hKCC1)
Length = 1085
Score = 209 bits (531), Expect = 1e-53
Identities = 114/260 (43%), Positives = 160/260 (60%), Gaps = 25/260 (9%)
Query: 180 GITGLSLETLKDNWGSEYQKTNDA----GIPEPDG-------------SVSWNFNALVGL 222
GI G + L++N S Y + D G+P D ++ +F LVG+
Sbjct: 367 GIPGAAAGVLQENLWSAYLEKGDIVEKHGLPSADAPSLKESLPLYVVADIATSFTVLVGI 426
Query: 223 FFPAVTGIMAGSNRSSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL 278
FFP+VTGIMAGSNRS L+D Q+SIP+GT+ A + T+ +Y SV++FGA + R+K
Sbjct: 427 FFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSLVYFSSVVLFGACIEGVVLRDKY 486
Query: 279 ---LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFK 335
++ L+ T+AWP P +I IG ST GA LQSLTGAPRLL AIA D+I+P L+ F
Sbjct: 487 GDGVSRNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG 546
Query: 336 VAD-GSEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPS 394
EP A L TA + ++I +LD++ P ++MFFL+CY VNL+C + LL P+
Sbjct: 547 HGKVNGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPN 606
Query: 395 WRPRWKFHHWSLSLVGALLC 414
WRPR+K++HW+LS +G LC
Sbjct: 607 WRPRFKYYHWALSFLGMSLC 626
Score = 130 bits (327), Expect = 6e-30
Identities = 71/180 (39%), Positives = 106/180 (58%), Gaps = 9/180 (5%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG G+ LL+V +C CT LTAIS+SAIATNG + GG Y++I R+LGPE G ++GL
Sbjct: 144 MVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGL 203
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFRETITQVNGTTIAQPIESPSSHDLQIYG 129
CF+LG A A+Y+LGA+E L + P A IF + A + + +++++YG
Sbjct: 204 CFYLGTTFAAAMYILGAIEILLTYIAPPAAIFY--------PSGAHDTSNATLNNMRVYG 255
Query: 130 IVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETL 189
+ + +VF GVK +N+ A FL V+ S++ IY G + + D P + L TL
Sbjct: 256 TIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTL 315
>S124_RAT (Q63632) Solute carrier family 12 member 4 (Electroneutral
potassium-chloride cotransporter 1) (Erythroid K-Cl
cotransporter 1) (rKCC1) (Furosemide-sensitive K-Cl
cotransporter)
Length = 1085
Score = 207 bits (528), Expect = 3e-53
Identities = 113/260 (43%), Positives = 161/260 (61%), Gaps = 25/260 (9%)
Query: 180 GITGLSLETLKDNWGSEYQKTNDA----GIPEPD-------------GSVSWNFNALVGL 222
GI G + L++N S Y + + G+P D ++ +F LVG+
Sbjct: 367 GIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSFTVLVGI 426
Query: 223 FFPAVTGIMAGSNRSSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL 278
FFP+VTGIMAGSNRS L+D Q+SIP+GT+ A + T+ +Y SVI+FGA + R+K
Sbjct: 427 FFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKY 486
Query: 279 ---LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFK 335
++ L+ T+AWP P +I +G ST GA LQSLTGAPRLL AIA D+I+P L+ F
Sbjct: 487 GDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG 546
Query: 336 VADGS-EPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPS 394
+ EP A L TA + ++I +LD++ P ++MFFL+CY VNL+C + LL P+
Sbjct: 547 HGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPN 606
Query: 395 WRPRWKFHHWSLSLVGALLC 414
WRPR+K++HW+LS +G LC
Sbjct: 607 WRPRFKYYHWALSFLGMSLC 626
Score = 134 bits (337), Expect = 4e-31
Identities = 72/180 (40%), Positives = 108/180 (60%), Gaps = 9/180 (5%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG G+ LL+V +C CT LTAIS+SAIATNG + GG Y++I R+LGPE G ++GL
Sbjct: 144 MVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGL 203
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFRETITQVNGTTIAQPIESPSSHDLQIYG 129
CF+LG A A+Y+LGA+E L + P A IF + T + S + +++++YG
Sbjct: 204 CFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGT--------HDMSSATLNNMRVYG 255
Query: 130 IVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETL 189
+ + +VF GVK +N+ A FL V+ S++ IY+G + + D P + L TL
Sbjct: 256 TIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYVGGIKSAFDPPVFPVCMLGNRTL 315
>S124_MOUSE (Q9JIS8) Solute carrier family 12 member 4
(Electroneutral potassium-chloride cotransporter 1)
(Erythroid K-Cl cotransporter 1) (mKCC1)
Length = 1085
Score = 207 bits (528), Expect = 3e-53
Identities = 113/260 (43%), Positives = 161/260 (61%), Gaps = 25/260 (9%)
Query: 180 GITGLSLETLKDNWGSEYQKTNDA----GIPEPD-------------GSVSWNFNALVGL 222
GI G + L++N S Y + + G+P D ++ +F LVG+
Sbjct: 367 GIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSFTVLVGI 426
Query: 223 FFPAVTGIMAGSNRSSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL 278
FFP+VTGIMAGSNRS L+D Q+SIP+GT+ A + T+ +Y SVI+FGA + R+K
Sbjct: 427 FFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKY 486
Query: 279 ---LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFK 335
++ L+ T+AWP P +I +G ST GA LQSLTGAPRLL AIA D+I+P L+ F
Sbjct: 487 GDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG 546
Query: 336 VADGS-EPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPS 394
+ EP A L TA + ++I +LD++ P ++MFFL+CY VNL+C + LL P+
Sbjct: 547 HGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPN 606
Query: 395 WRPRWKFHHWSLSLVGALLC 414
WRPR+K++HW+LS +G LC
Sbjct: 607 WRPRFKYYHWTLSFLGMSLC 626
Score = 133 bits (335), Expect = 7e-31
Identities = 72/180 (40%), Positives = 108/180 (60%), Gaps = 9/180 (5%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG G+ LL+V +C CT LTAIS+SAIATNG + GG Y++I R+LGPE G ++GL
Sbjct: 144 MVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGL 203
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFRETITQVNGTTIAQPIESPSSHDLQIYG 129
CF+LG A A+Y+LGA+E L + P A IF + T + S + +++++YG
Sbjct: 204 CFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGT--------HDMSSATLNNMRVYG 255
Query: 130 IVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETL 189
+ ++ +VF GVK +N+ A FL V+ S++ IY G + + D P + L TL
Sbjct: 256 TIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTL 315
>S126_MOUSE (Q924N4) Solute carrier family 12 member 6
(Electroneutral potassium-chloride cotransporter 3)
(K-Cl cotransporter 3)
Length = 1150
Score = 207 bits (527), Expect = 4e-53
Identities = 116/261 (44%), Positives = 163/261 (62%), Gaps = 27/261 (10%)
Query: 179 EGITGLSLETLKDNWGSEYQKTNDAGIPEPDGS----------------VSWNFNALVGL 222
+GI GL+ + +N S Y + I +P ++ +F LVG+
Sbjct: 433 QGIPGLASGIITENLWSNYLPKGEI-IEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGI 491
Query: 223 FFPAVTGIMAGSNRSSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL 278
FFP+VTGIMAGSNRS LKD Q+SIP+GT+ A L T+F+YL +V++FGA + R+K
Sbjct: 492 FFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKF 551
Query: 279 ---LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYF- 334
+ L+ T++WP P +I IG ST GA LQSLTGAPRLL AIA D+I+P L+ F
Sbjct: 552 GDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG 611
Query: 335 -KVADGSEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAP 393
A+G EP A L TA + ++I +LDL+ P ++MFFL+CY VNL+C L LL P
Sbjct: 612 HSKANG-EPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTP 670
Query: 394 SWRPRWKFHHWSLSLVGALLC 414
+WRPR++++HW+LS +G +C
Sbjct: 671 NWRPRFRYYHWALSFMGMSIC 691
Score = 131 bits (329), Expect = 4e-30
Identities = 81/220 (36%), Positives = 116/220 (51%), Gaps = 16/220 (7%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG GI +V +C CT LTAIS+SAIATNG + GG Y++I RALGPE G ++GL
Sbjct: 211 VVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGL 270
Query: 71 CFFLGNAVAGALYVLGAVETFL-KAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYG 129
CF+LG A A+Y+LGA+E FL VP A IFR + A + +++++YG
Sbjct: 271 CFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFR--------SDDALKESAAMLNNMRVYG 322
Query: 130 IVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETL 189
+++ +VF GV+ +N+ A FL V+ S++ IY G + + P + L TL
Sbjct: 323 TAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTL 382
Query: 190 KDNWGSEYQKTNDAG---IPEPDGSVSWNFNALVGLFFPA 226
KT + +P S W F FF A
Sbjct: 383 SSRHLDICSKTKEVDNMTVP----SKLWGFFCNSSQFFNA 418
>S126_HUMAN (Q9UHW9) Solute carrier family 12 member 6
(Electroneutral potassium-chloride cotransporter 3)
(K-Cl cotransporter 3)
Length = 1150
Score = 207 bits (527), Expect = 4e-53
Identities = 116/261 (44%), Positives = 163/261 (62%), Gaps = 27/261 (10%)
Query: 179 EGITGLSLETLKDNWGSEYQKTNDAGIPEPDGS----------------VSWNFNALVGL 222
+GI GL+ + +N S Y + I +P ++ +F LVG+
Sbjct: 433 QGIPGLASGIITENLWSNYLPKGEI-IEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGI 491
Query: 223 FFPAVTGIMAGSNRSSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL 278
FFP+VTGIMAGSNRS LKD Q+SIP+GT+ A L T+F+YL +V++FGA + R+K
Sbjct: 492 FFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKF 551
Query: 279 ---LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYF- 334
+ L+ T++WP P +I IG ST GA LQSLTGAPRLL AIA D+I+P L+ F
Sbjct: 552 GDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG 611
Query: 335 -KVADGSEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAP 393
A+G EP A L TA + ++I +LDL+ P ++MFFL+CY VNL+C L LL P
Sbjct: 612 HSKANG-EPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTP 670
Query: 394 SWRPRWKFHHWSLSLVGALLC 414
+WRPR++++HW+LS +G +C
Sbjct: 671 NWRPRFRYYHWALSFMGMSIC 691
Score = 130 bits (327), Expect = 6e-30
Identities = 80/220 (36%), Positives = 116/220 (52%), Gaps = 16/220 (7%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG G+ +V +C CT LTAIS+SAIATNG + GG Y++I RALGPE G ++GL
Sbjct: 211 VVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGL 270
Query: 71 CFFLGNAVAGALYVLGAVETFL-KAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYG 129
CF+LG A A+Y+LGA+E FL VP A IF + A + +++++YG
Sbjct: 271 CFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFH--------SDDALKESAAMLNNMRVYG 322
Query: 130 IVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETL 189
+++ +VF GV+ +N+ A FL V+ S++ IY G + + P + L TL
Sbjct: 323 TAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTL 382
Query: 190 KDNWGSEYQKT---NDAGIPEPDGSVSWNFNALVGLFFPA 226
KT N+ +P S W F FF A
Sbjct: 383 SSRHIDVCSKTKEINNMTVP----SKLWGFFCNSSQFFNA 418
>S124_RABIT (Q28677) Solute carrier family 12 member 4
(Electroneutral potassium-chloride cotransporter 1)
(Erythroid K-Cl cotransporter 1) (rbKCC1)
Length = 1085
Score = 207 bits (527), Expect = 4e-53
Identities = 112/260 (43%), Positives = 161/260 (61%), Gaps = 25/260 (9%)
Query: 180 GITGLSLETLKDNWGSEYQKTNDA----GIPEPDG-------------SVSWNFNALVGL 222
GI G + L++N S Y + + G+P D ++ +F LVG+
Sbjct: 367 GIPGAAAGVLQENLWSAYLEKGEVVEKRGLPSTDAVGLKENLPLYVVADIATSFTVLVGI 426
Query: 223 FFPAVTGIMAGSNRSSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA----LATREKL 278
FFP+VTGIMAGSNRS L+D Q+SIP+GT+ A + T+ +Y SV++FGA + R+K
Sbjct: 427 FFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVVLFGACIEGVVLRDKY 486
Query: 279 ---LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFK 335
++ L+ T+AWP P +I +G ST GA LQSLTGAPRLL AIA D+I+P L+ F
Sbjct: 487 GDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG 546
Query: 336 VADGS-EPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPS 394
+ EP A L TA + ++I +LD++ P ++MFFL+CY VNL+C + LL P+
Sbjct: 547 HGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPN 606
Query: 395 WRPRWKFHHWSLSLVGALLC 414
WRPR+K++HW+LS +G LC
Sbjct: 607 WRPRFKYYHWALSFLGMSLC 626
Score = 131 bits (330), Expect = 3e-30
Identities = 72/180 (40%), Positives = 106/180 (58%), Gaps = 9/180 (5%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VG G+ LL+V +C CT LTAIS+SAIATNG + GG Y++I R+LGPE G ++GL
Sbjct: 144 MVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGL 203
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFRETITQVNGTTIAQPIESPSSHDLQIYG 129
CF+LG A A+Y+LGA+E L + P A IF + T + + +++++YG
Sbjct: 204 CFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGT--------HDTSNATLNNMRVYG 255
Query: 130 IVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETL 189
V + +VF GVK +N+ A FL V+ S++ IY G + + D P + L TL
Sbjct: 256 TVFLSFMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSMFDPPVFPVCMLGNRTL 315
>S127_HUMAN (Q9Y666) Solute carrier family 12 member 7
(Electroneutral potassium-chloride cotransporter 4)
(K-Cl cotransporter 4)
Length = 1083
Score = 201 bits (511), Expect = 3e-51
Identities = 103/211 (48%), Positives = 141/211 (66%), Gaps = 8/211 (3%)
Query: 212 VSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGA 271
++ +F LVG++FP+VTGIMAGSNRS LKD Q+SIP GT+ A + T+F+YL +++FGA
Sbjct: 416 IAASFTLLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGA 475
Query: 272 ----LATREKL---LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIAN 324
+ R+K L L+ +AWP P +I IG ST GA LQ+LTGAPRLL AIA
Sbjct: 476 CIEGVVLRDKFGEALQGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQTLTGAPRLLQAIAR 535
Query: 325 DDILPILKYFKVADGS-EPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLS 383
D I+P L+ F + EP A L T +C ++I +LD + P ++MFFL+CY VNL+
Sbjct: 536 DGIVPFLQVFGHGKANGEPTWALLLTVLICETGILIASLDSVAPILSMFFLMCYLFVNLA 595
Query: 384 CFLLDLLDAPSWRPRWKFHHWSLSLVGALLC 414
C + LL P+WRPR+KF+HW+LS +G LC
Sbjct: 596 CAVQTLLRTPNWRPRFKFYHWTLSFLGMSLC 626
Score = 147 bits (371), Expect = 5e-35
Identities = 77/180 (42%), Positives = 114/180 (62%), Gaps = 9/180 (5%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
IVG+ G+ + L+VA+C TCT LTAIS+SAIATNG + GG YY+I R+LGPE G ++GL
Sbjct: 144 IVGVAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGL 203
Query: 71 CFFLGNAVAGALYVLGAVETFLKAV-PAAGIFRETITQVNGTTIAQPIESPSSHDLQIYG 129
CF+LG AGA+Y+LG +E FL + P A IF+ + G A + H++++YG
Sbjct: 204 CFYLGTTFAGAMYILGTIEIFLTYISPGAAIFQ---AEAAGGEAAAML-----HNMRVYG 255
Query: 130 IVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGITGLSLETL 189
+++ +VF GVK +N++A FL V+ S++ IY G++ + D P + L TL
Sbjct: 256 TCTLVLMALVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKSAFDPPDIPVCLLGNRTL 315
>S121_HUMAN (Q13621) Solute carrier family 12 member 1
(Bumetanide-sensitive sodium-(potassium)-chloride
cotransporter 2) (Kidney-specific Na-K-Cl symporter)
Length = 1099
Score = 201 bits (510), Expect = 4e-51
Identities = 132/443 (29%), Positives = 210/443 (46%), Gaps = 68/443 (15%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
IVG GIG ++++ L T +T +S SAIATNG ++GGG YYLI R+LGPE G SIGL
Sbjct: 203 IVGEAGIGLGVIIIGLSTIVTTITGMSTSAIATNGVVRGGGAYYLISRSLGPEFGGSIGL 262
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYGI 130
F NAVA A+YV+G ET + + + + + + P ++D++I G
Sbjct: 263 IFAFANAVAVAMYVVGFAETVV-----------DLLKESDSMMVDP-----TNDIRIIGS 306
Query: 131 VVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLG-ILLAREDHPAEGITGLSLETL 189
+ ++L I G++ + L+ +L ++ ++G ++ + + + G
Sbjct: 307 ITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIF 366
Query: 190 KDNWGSEYQKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSIPL 249
+N+G + K F ++ +FFPA TGI+AG+N S L+D Q +IP
Sbjct: 367 AENFGPRFTKGE-------------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPR 413
Query: 250 GTLAATLVTTFMYLVSVIMFGALATREKL------------------------------- 278
GT+ A +TT YL I GA R+
Sbjct: 414 GTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHE 473
Query: 279 -----LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKY 333
L + ++ F LI GI +T+ +AL SL AP++ A+ D+I L++
Sbjct: 474 PCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQF 533
Query: 334 FKVADG--SEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLD 391
F G +EP + T + ++I L+ I P ++ FFL YA +N SCF
Sbjct: 534 FAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAK 593
Query: 392 APSWRPRWKFHHWSLSLVGALLC 414
+P WRP + ++ +SL GA+LC
Sbjct: 594 SPGWRPAYGIYNMWVSLFGAVLC 616
>S123_PSEAM (P55019) Solute carrier family 12 member 3
(Thiazide-sensitive sodium-chloride cotransporter)
(Na-Cl symporter)
Length = 1023
Score = 197 bits (501), Expect = 4e-50
Identities = 142/446 (31%), Positives = 208/446 (45%), Gaps = 70/446 (15%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
I GIG T +++ L T +T +S SAIATNG +KGGG Y+LI R+LGPE+G SIGL
Sbjct: 159 ITAQAGIGLTWVIILLSSFITGITGLSTSAIATNGKVKGGGTYFLISRSLGPELGGSIGL 218
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYGI 130
F NAVA A++ +G ET + RE NG + PI +D++I G+
Sbjct: 219 IFAFANAVAVAMHTVGFAET------VTDLMRE-----NGVVMVDPI-----NDIRIVGV 262
Query: 131 VVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLG-ILLAREDHPAEGITGLSLETL 189
+ L I G++ ++ F + ++ S + +G I+ A A+G E
Sbjct: 263 ITVTCLLGISMAGMEWESKAQVLFFLVIMVSFVNYIVGTIIPASPQKQAKGFFSYKAEIF 322
Query: 190 KDNWGSEYQKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSIPL 249
N+ +P G +F + +FFP+ TGI+AG+N S LKD +IP
Sbjct: 323 AANF-----------VPGWRGK-EGSFFGMFSIFFPSATGILAGANISGDLKDPTVAIPR 370
Query: 250 GTLAATLVTTFMYLVSVIMFGALATREKL--LTDRL-----------LTATVAWPFPS-- 294
GTL A TT YL+ GA R+ L D L L W F
Sbjct: 371 GTLMAIFWTTISYLIISATIGACVVRDASGELNDTLSYSSSSENCSGLACQYRWDFSECI 430
Query: 295 ------------------------LIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPI 330
LI GI +T+ +AL L AP++ + D + P+
Sbjct: 431 KNNTCKHGIMNYYQSMSLVSAFAPLISAGIFGATLSSALACLVSAPKVFQCLCKDQLYPL 490
Query: 331 LKYFKVADG--SEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLD 388
+ +F G +EP A L T + V+I L+ I P ++ FFL YA +N SCF
Sbjct: 491 IGFFGKGYGKNAEPLRAYLLTYVIAVCFVLIAELNTIAPIISNFFLCSYALINFSCFHAS 550
Query: 389 LLDAPSWRPRWKFHHWSLSLVGALLC 414
+ ++P WRP ++F+ LSL+GA+ C
Sbjct: 551 VTNSPGWRPSFRFYSKWLSLLGAVCC 576
>S121_RABIT (P55015) Solute carrier family 12 member 1
(Bumetanide-sensitive sodium-(potassium)-chloride
cotransporter 2) (Kidney-specific Na-K-Cl symporter)
Length = 1099
Score = 196 bits (497), Expect = 1e-49
Identities = 131/443 (29%), Positives = 209/443 (46%), Gaps = 68/443 (15%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
IVG GIG ++++ L T +T +S SAIATNG ++GGG YYLI R+LGPE G SIGL
Sbjct: 203 IVGEAGIGLGVVIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGL 262
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYGI 130
F NAVA A+YV+G ET + + + + + + P ++D++I G
Sbjct: 263 IFAFANAVAVAMYVVGFAETVV-----------DLLKESDSMMVDP-----TNDIRIIGS 306
Query: 131 VVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLG-ILLAREDHPAEGITGLSLETL 189
+ ++L I G++ + LI +L ++ ++G ++ + + + G
Sbjct: 307 ITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIF 366
Query: 190 KDNWGSEYQKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSIPL 249
+N+G + K F ++ +FFPA TGI+AG+N S L+D Q +IP
Sbjct: 367 AENFGPSFTKGE-------------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPR 413
Query: 250 GTLAATLVTTFMYLVSVIMFGALATREKL------------------------------- 278
GT+ A +TT Y+ I GA R+
Sbjct: 414 GTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGINCNGSAACGLGYDFSRCRHE 473
Query: 279 -----LTDRLLTATVAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKY 333
L + ++ F LI GI +T+ +AL SL A ++ A+ D+I L++
Sbjct: 474 PCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQF 533
Query: 334 FKVADG--SEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLD 391
F G +EP + T + ++I L+ I P ++ FFL YA +N SCF
Sbjct: 534 FAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAK 593
Query: 392 APSWRPRWKFHHWSLSLVGALLC 414
+P WRP + ++ +SL GA+LC
Sbjct: 594 SPGWRPAYGIYNMWVSLFGAVLC 616
>NKCL_MANSE (Q25479) Bumetanide-sensitive
sodium-(Potassium)-chloride cotransporter (NA-K-CL
symporter)
Length = 1060
Score = 189 bits (481), Expect = 9e-48
Identities = 130/440 (29%), Positives = 218/440 (49%), Gaps = 61/440 (13%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+V GIG +L+++A+ +T +S+SAI TNG +KGGG YY+I R+LGPE G S+G+
Sbjct: 149 VVSQAGIGLSLVIIAISAIVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGI 208
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQ-PIESPSSHDLQIYG 129
F NAVA ++ +G ++ + + NG I + PI +D++I G
Sbjct: 209 IFAFANAVAASMNTIGFCDSL-----------NDLLRSNGLKITEDPI-----NDVRIVG 252
Query: 130 IVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHP--AEGITGLSLE 187
V +V+C I G+ ++ + ++ +++ +G ++ +D+ A+G GLS
Sbjct: 253 TVALLVMCIICAIGMDWESKAQNFLIAIIVGAMVDFVVGTIMGPKDNSEIAKGFVGLSSA 312
Query: 188 TLKDNWGSEYQKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSI 247
T +N+ S+++ + + NF ++ +FFP+VTGI AG+N S LKD +I
Sbjct: 313 TFVENFKSDFRFSE---------KLDQNFFSVFAIFFPSVTGIQAGANISGDLKDPASAI 363
Query: 248 PLGTLAATLVTTFMYLVSVIMFGALATREKL--LTDRLLTATVAWPFPSL---------- 295
P GTL A L++ Y + V+ G A R+ +TD L+ + S+
Sbjct: 364 PKGTLLALLISMVSYTLMVLFAGGGALRDASGNITDLLIVNGTVTDYSSVSLCALNNTCE 423
Query: 296 -------------------IKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYFKV 336
I G +T+ AL +L PRL+ A+ D I P L +F
Sbjct: 424 YGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSK 483
Query: 337 ADG--SEPHVATLFTAFLCSGCVVIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPS 394
G EP+ + T F+ ++I +L+ I P ++ F+L YA +N F L+
Sbjct: 484 PYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLG 543
Query: 395 WRPRWKFHHWSLSLVGALLC 414
WRP +++++ LSL G L+C
Sbjct: 544 WRPTFRYYNMWLSLAGFLMC 563
>YB85_YEAST (P38329) Hypothetical 124.0 kDa protein in PBP2-ABD1
intergenic region
Length = 1120
Score = 178 bits (451), Expect = 3e-44
Identities = 132/406 (32%), Positives = 201/406 (48%), Gaps = 22/406 (5%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
I+G GI T+ L+ L T LT +S+SAI+TNG ++GGG YY+I R+LGPE G SIGL
Sbjct: 89 ILGQLGIICTIGLLLLSYTINLLTTLSISAISTNGTVRGGGAYYMISRSLGPEFGGSIGL 148
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQPIESPSSHDLQIYGI 130
FFLG + +G +E L + + A P H +
Sbjct: 149 VFFLGQVFNAGMNAVGIIEPLL--------YNLGYSAQGEPPAALGELLPRGHWHEFTYA 200
Query: 131 VVTIVLCF-IVFGGVKMINRVAPAFLIPVLFSLICIYLGILLAREDHPAEGI--TGLSLE 187
V + LCF + F G + ++R + + S+ I L L+ R GI TG S +
Sbjct: 201 TVILFLCFSVAFVGSQTVSRAGNILFLVLAASIFSIPLSALI-RSPFTEGGISYTGPSWQ 259
Query: 188 TLKDNWGSEYQKTNDAGIPEPDGSVSWNFNALVGLFFPAVTGIMAGSNRSSSLKDTQRSI 247
T DN K + + + FN L G+FFPA GI AG+ SS L+ +SI
Sbjct: 260 TFHDNLLPHLTKGAAGSLLKGKET----FNDLFGVFFPATAGIFAGAGMSSELRKPSKSI 315
Query: 248 PLGTLAATLVTTFMYLVSVIMFGALATREKLLTDRLLTATVAWPFPSLIKIGIILSTMGA 307
P GTL L T Y V V G R L + + T++ +I +G + +++ +
Sbjct: 316 PKGTLWGLLFTFICYAVVVFSMGCSIPRRSLYDEVQIIQTIS-SVQWVIFMGEMATSLFS 374
Query: 308 ALQSLTGAPRLLAAIANDDILPILKYFKVADGSEPHVATLFTAFLCSGCVVIGNLDLITP 367
+ + GA +L AIA D+I+P L+ F P + +FT L C + +++ I
Sbjct: 375 IIVGMLGAAYVLEAIAKDNIIPGLEIF----AHSPLYSLIFTWILTQLC-LFSDVNKIAT 429
Query: 368 TVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLVGALL 413
+TM FL+ + +NL+CFLL + AP++RP +K+ + + +GALL
Sbjct: 430 FITMTFLMTFVVMNLACFLLGISSAPNFRPSFKYFNRYTTAIGALL 475
>YRD3_CAEEL (Q09573) Hypothetical protein K02A2.3 in chromosome II
Length = 1020
Score = 145 bits (366), Expect = 2e-34
Identities = 85/229 (37%), Positives = 128/229 (55%), Gaps = 19/229 (8%)
Query: 183 GLSLETLKDNWGSEYQKTNDAGIPEPDGSV--------SWNFNALVGLFFPAVTGIMAGS 234
G +++TL DN EY + ++ +P G S F L+ ++FPAVTGI G+
Sbjct: 339 GFNMKTLNDNMWPEYMEKSEV-VPGVRGKETAEVVQDESSTFFMLMAIYFPAVTGIFTGT 397
Query: 235 NRSSSLKDTQRSIPLGTLAATLVTTFMYLVSVIMFGALATREKL-------LTDRLLTAT 287
N S L+D QRSIP+GT+AATL T+ +Y + I+FG TR L + + ++ A
Sbjct: 398 NMSGDLRDPQRSIPVGTIAATLTTSAIYYILAILFGGSITRSVLRDKFGRSIGNTMVVAA 457
Query: 288 VAWPFPSLIKIGIILSTMGAALQSLTGAPRLLAAIANDDILPILKYF-KVADGSEPHVAT 346
++WP P+++ +G LST GAALQ L APRLL +IA DD++PIL F +V +EP +
Sbjct: 458 LSWPHPAVVTVGAFLSTFGAALQCLCSAPRLLQSIAKDDVIPILAPFARVTKNNEPFLGL 517
Query: 347 LFTAFLCSGCVVIGNLDLITPTVTMFFL--LCYAGVNLSCFLLDLLDAP 393
+ T + +++G +D I + FFL L G L F++ P
Sbjct: 518 VLTVIIAECGILLGAVDKIAEVLDFFFLMTLSLLGAALCFFIMFASSVP 566
Score = 106 bits (264), Expect = 1e-22
Identities = 60/162 (37%), Positives = 94/162 (57%), Gaps = 10/162 (6%)
Query: 11 IVGMGGIGGTLLLVALCGTCTFLTAISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 70
+VGM G+ T+ L+A+C T LT+ISLSA+ATNG ++ GG Y++I R LG E G ++G+
Sbjct: 96 VVGMSGVAWTMALLAICCLSTLLTSISLSAVATNGVVESGGAYFIISRNLGAEFGSAVGI 155
Query: 71 CFFLGNAVAGALYVLGAVETFLKAVPAAGIFRETITQVNGTTIAQPIESPSS--HDLQIY 128
F+L N VA ++Y++G VE L ++ + G E S ++L++Y
Sbjct: 156 LFYLANTVAASMYIVGGVEVIL-------MYLWPEMAIGGADALHDTEMFGSLYNNLRLY 208
Query: 129 GIVVTIVLCFIVFGGVKMINRVAPAFLIPVLFSL-ICIYLGI 169
G V ++ IV GVK + +AP L+ V+ ++ CI GI
Sbjct: 209 GTVFLLIQALIVAMGVKFVQLLAPVSLMCVILAIAACIGGGI 250
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.142 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,524,151
Number of Sequences: 164201
Number of extensions: 2011139
Number of successful extensions: 6745
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 39
Number of HSP's that attempted gapping in prelim test: 6614
Number of HSP's gapped (non-prelim): 106
length of query: 414
length of database: 59,974,054
effective HSP length: 113
effective length of query: 301
effective length of database: 41,419,341
effective search space: 12467221641
effective search space used: 12467221641
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 67 (30.4 bits)
Lotus: description of TM0140.12