BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0134.14
         (277 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

E2K3_DROME (Q9NIV1) Eukaryotic translation initiation factor 2-a...    33  0.99
GR78_PLAFO (Q05866) 78 kDa glucose-regulated protein homolog pre...    31  3.8
LONM_YEAST (P36775) Lon protease homolog, mitochondrial precurso...    30  4.9
GIDB_MYCPE (Q8EUW9) Methyltransferase gidB (EC 2.1.-.-) (Glucose...    30  6.4
GAL1_STACA (Q9RGS1) Galactokinase (EC 2.7.1.6) (Galactose kinase)      30  8.4
DYHC_EMENI (P45444) Dynein heavy chain, cytosolic (DYHC)               30  8.4

>E2K3_DROME (Q9NIV1) Eukaryotic translation initiation factor 2-alpha
            kinase precursor (EC 2.7.1.-) (PRKR-like endoplasmic
            reticulum kinase) (PERK) (PEK) (DmPEK)
          Length = 1162

 Score = 32.7 bits (73), Expect = 0.99
 Identities = 23/63 (36%), Positives = 31/63 (48%), Gaps = 3/63 (4%)

Query: 168  SKLESLAKHFQFFNNHVDERYMCKRFLNGLRADIKDSVRPLGIIRFQALVEKATEVELMK 227
            S L S A+H Q    H+   YM    L G   D K  +  LG+I F+  V  +TE+E +K
Sbjct: 1019 SGLPSCARHTQQVGTHL---YMSPEQLLGQHYDYKVDIYSLGLIFFELHVYFSTEMERIK 1075

Query: 228  NRR 230
              R
Sbjct: 1076 TLR 1078


>GR78_PLAFO (Q05866) 78 kDa glucose-regulated protein homolog
           precursor (GRP 78)
          Length = 655

 Score = 30.8 bits (68), Expect = 3.8
 Identities = 26/110 (23%), Positives = 49/110 (43%), Gaps = 8/110 (7%)

Query: 80  IQEIEKIFEVLQTAEGAKVSLATYLLLGDAEYWWKGTRGIMRANHEEVN*NSFRTAF--L 137
           IQ++ K  E+ +      V  +T + + D       +  + RA  EE+N + FR     +
Sbjct: 283 IQKLRKEVEIAK--RNLSVVHSTQIEIEDIVEGHNFSETLTRAKFEELNDDLFRETLEPV 340

Query: 138 EKYFPTSARDEREAQFLTLGQGSMTVPEYASKLESLAKHFQFFNNHVDER 187
           +K    +  ++ +   + L  GS  +P    K++ + K F+FFN     R
Sbjct: 341 KKVLDDAKYEKSKIDEIVLVGGSTRIP----KIQQIIKEFEFFNGKEPNR 386


>LONM_YEAST (P36775) Lon protease homolog, mitochondrial precursor
           (EC 3.4.21.-)
          Length = 1133

 Score = 30.4 bits (67), Expect = 4.9
 Identities = 21/63 (33%), Positives = 29/63 (45%), Gaps = 2/63 (3%)

Query: 200 DIKDSVRPLGIIRFQALVEKATEVELMKNRRMNGAGTGGPMRSSSQDNLGKGRFQMKKPY 259
           D KD+   +   R   +V K  E E  KN R   + +GG   SSS+ + G G  + K P 
Sbjct: 116 DEKDTDNDVEPTRDDEIVNKDQEGEASKNSR--SSASGGGQSSSSRSDSGDGSSKQKPPK 173

Query: 260 QRP 262
             P
Sbjct: 174 DVP 176


>GIDB_MYCPE (Q8EUW9) Methyltransferase gidB (EC 2.1.-.-) (Glucose
           inhibited division protein B)
          Length = 237

 Score = 30.0 bits (66), Expect = 6.4
 Identities = 29/102 (28%), Positives = 44/102 (42%), Gaps = 14/102 (13%)

Query: 147 DEREAQFLTLGQGSMTVPEYASKL----------ESLAKHFQFFNNHVDERYMCKRFLNG 196
           D +    L +G GS  VP    K+          ES  K   F NN VD+  +   F++ 
Sbjct: 69  DNKNINLLDIGSGS-GVPGIFLKILFPHINLYIVESNTKKVNFLNNLVDKLELENVFISN 127

Query: 197 LRAD--IKDSVRPLGIIRFQALVEKATEVEL-MKNRRMNGAG 235
            R +  IKD +    +I  +A+ E    +EL     ++NG G
Sbjct: 128 QRCEDYIKDKIEFFDLITCRAVAELRILLELSFPGLKINGIG 169


>GAL1_STACA (Q9RGS1) Galactokinase (EC 2.7.1.6) (Galactose kinase)
          Length = 388

 Score = 29.6 bits (65), Expect = 8.4
 Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 1/47 (2%)

Query: 192 RFLNGLRADIKDSVRPLGIIRFQALVEKATEVELMKNRRMNGAGTGG 238
           R LN   A +K+     GI     L E A +VE +   RM GAG  G
Sbjct: 297 RLLNASHASLKNDYEVTGI-ELDTLAETAQQVEGVLGARMTGAGFAG 342


>DYHC_EMENI (P45444) Dynein heavy chain, cytosolic (DYHC)
          Length = 4344

 Score = 29.6 bits (65), Expect = 8.4
 Identities = 27/106 (25%), Positives = 46/106 (42%), Gaps = 5/106 (4%)

Query: 19   VQAMTVQTNDNAHRRAT--EDTRELHRLQREVALDQNRGLNDFRRQDPPKFTGGTDPDNA 76
            +Q M     +   R+A   E    L + ++EVAL ++  L+D  R +P          N 
Sbjct: 3235 LQRMVADQREAEQRKAVSLEVQAALEKQEKEVALRKDVVLHDLARAEPAVLEAQKSVSNI 3294

Query: 77   DL*IQEIEKIFEVLQTAEGAKVSL-ATYLLLGDAEYWWKGTRGIMR 121
                Q + ++  +     G +++L A   LLG     WK  +GI+R
Sbjct: 3295 KR--QHLTEVRSMGNPPAGVRLALEAVCTLLGHKVDSWKTIQGIVR 3338


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.324    0.138    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,858,334
Number of Sequences: 164201
Number of extensions: 1273150
Number of successful extensions: 3269
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 3269
Number of HSP's gapped (non-prelim): 8
length of query: 277
length of database: 59,974,054
effective HSP length: 109
effective length of query: 168
effective length of database: 42,076,145
effective search space: 7068792360
effective search space used: 7068792360
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 65 (29.6 bits)