BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0132.5
         (458 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

NFS1_ARATH (O49543) Cysteine desulfurase, mitochondrial precurso...   716  0.0
NFS1_HUMAN (Q9Y697) Cysteine desulfurase, mitochondrial precurso...   594  e-169
NFS1_RAT (Q99P39) Cysteine desulfurase, mitochondrial precursor ...   590  e-168
NFS1_MOUSE (Q9Z1J3) Cysteine desulfurase, mitochondrial precurso...   585  e-167
NFS1_DROME (Q9VKD3) Probable cysteine desulfurase, mitochondrial...   582  e-166
ISCS_RICCN (Q92HP1) Cysteine desulfurase (EC 2.8.1.7)                 545  e-155
ISCS_RICPR (Q9ZD60) Cysteine desulfurase (EC 2.8.1.7) (NifS prot...   544  e-154
NFS1_CANAL (P87185) Cysteine desulfurase, mitochondrial precurso...   528  e-150
NFS1_ASHGO (O60028) Cysteine desulfurase, mitochondrial precurso...   528  e-149
NFS1_SCHPO (O74351) Probable cysteine desulfurase, mitochondrial...   526  e-149
NFS1_CANMA (P87187) Cysteine desulfurase, mitochondrial precurso...   526  e-149
NFS1_YEAST (P25374) Cysteine desulfurase, mitochondrial precurso...   516  e-146
ISCS_VIBPA (Q87S28) Cysteine desulfurase (EC 2.8.1.7)                 501  e-141
ISCS_VIBVU (Q8DEY7) Cysteine desulfurase (EC 2.8.1.7)                 495  e-140
ISCS_VIBCH (Q9KTY2) Cysteine desulfurase (EC 2.8.1.7) (NifS prot...   489  e-138
ISCS_NEIMB (Q9JYY0) Cysteine desulfurase (EC 2.8.1.7) (NifS prot...   481  e-135
ISCS_SALTY (Q8ZN40) Cysteine desulfurase (EC 2.8.1.7) (ThiI tran...   479  e-135
ISCS_NEIMA (Q9JTX0) Cysteine desulfurase (EC 2.8.1.7) (NifS prot...   478  e-134
ISCS_SALTI (Q8Z4N0) Cysteine desulfurase (EC 2.8.1.7) (ThiI tran...   478  e-134
ISCS_HAEIN (Q57337) Cysteine desulfurase (EC 2.8.1.7) (NifS prot...   478  e-134

>NFS1_ARATH (O49543) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7)
          Length = 453

 Score =  716 bits (1849), Expect = 0.0
 Identities = 358/459 (77%), Positives = 404/459 (87%), Gaps = 7/459 (1%)

Query: 1   MTSKLLASTLRHHLTKPTQLLRR-HFLSTAAAAAAAAEPQQHHDESAGLTMKGVKIAGRP 59
           M SK++++T+R  LTKP     R  +LSTAAAA        + DES  + MKGV+I+GRP
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAATEV----NYEDES--IMMKGVRISGRP 54

Query: 60  LYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGASP 119
           LYLD+QAT+P+DPRV DAM    I  YGNPHSRTH YGWE++ AVENAR QVA LI ASP
Sbjct: 55  LYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASP 114

Query: 120 KEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPV 179
           KEIVF SGATE+NN+++KGV+HFYK+ K+HVITTQTEHKCVLDSCRHLQQEGF+VTYLPV
Sbjct: 115 KEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPV 174

Query: 180 ESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQALG 239
           ++DGLVDLE LR AIRPDTGLVS+MAVNNEIGV QPMEEIG ICKE NVPFHTDAAQA+G
Sbjct: 175 KTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIG 234

Query: 240 KVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIPT 299
           K+PVDV+KWNV+LMS+S HKIYGPKGVGALY+RRRPRIR+EP MNGGGQERG+RSGT  T
Sbjct: 235 KIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGAT 294

Query: 300 PLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLSF 359
             +VG GAACE+AMKEMEYDEK I  LQERLLNG+REKLDGVVVNGSM+ RY GNLNLSF
Sbjct: 295 QQIVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSF 354

Query: 360 AYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEAE 419
           AYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGV+EDMAHTSIRFGIGRFTT+ E
Sbjct: 355 AYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEE 414

Query: 420 IDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           ID+A++LTV QVEKLREMSPLYEMVKEGI+IK+IQW+QH
Sbjct: 415 IDKAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453


>NFS1_HUMAN (Q9Y697) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (HUSSY-08)
          Length = 457

 Score =  594 bits (1532), Expect = e-169
 Identities = 283/401 (70%), Positives = 341/401 (84%)

Query: 58  RPLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGA 117
           RPLY+DVQAT+P+DPRVLDAMLP+ I+ YGNPHSRTH YGWES+ A+E AR QVASLIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 118 SPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYL 177
            P+EI+FTSGATESNNI+IKGV  FY+ +K+H+ITTQTEHKCVLDSCR L+ EGF VTYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 178 PVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQA 237
           PV+  G++DL++L AAI+PDT LVSVM VNNEIGV QP+ EIGRIC    V FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236

Query: 238 LGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTI 297
           +GK+P+DV    + LMS+SGHKIYGPKGVGA+Y+RRRPR+RVE   +GGGQERG+RSGT+
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296

Query: 298 PTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNL 357
           PTPLVVG+GAACEVA +EMEYD KRIS L ERL+  I + L  VV+NG  +  Y G +NL
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINL 356

Query: 358 SFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTE 417
           SFAYVEGESLLM LK+VA+SSGSACTSASLEPSYVLRA+G +ED+AH+SIRFGIGRFTTE
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 416

Query: 418 AEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
            E+D  ++  +  V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 417 EEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 457


>NFS1_RAT (Q99P39) Cysteine desulfurase, mitochondrial precursor (EC
           2.8.1.7)
          Length = 451

 Score =  590 bits (1521), Expect = e-168
 Identities = 278/401 (69%), Positives = 343/401 (85%)

Query: 58  RPLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGA 117
           RPLY+DV+AT+P+DPRVLDAMLP+ ++ YGNPHSRTH YGWES+ A+E AR QVASLIGA
Sbjct: 51  RPLYMDVRATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 110

Query: 118 SPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYL 177
            P+EI+FTSGATESNNI+IKGV  FY+ +K+H++TTQTEHKCVLDSCR L+ EGF VTYL
Sbjct: 111 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 170

Query: 178 PVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQA 237
           PV+  G++DL++L AAI+PDT LVSVM VNNEIGV QP+ EIG+IC    + FHTDAAQA
Sbjct: 171 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKLYFHTDAAQA 230

Query: 238 LGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTI 297
           +GK+P+DV    + LMS+SGHK+YGPKGVGA+Y+RRRPR+RVE   +GGGQERG+RSGT+
Sbjct: 231 VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 290

Query: 298 PTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNL 357
           PTPLVVG+GAACE+A +EMEYD KRIS L ERL+  I + L  VV+NG  ++ Y G +NL
Sbjct: 291 PTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQKIMKNLPDVVMNGDPKQHYPGCINL 350

Query: 358 SFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTE 417
           SFAYVEGESLLM LK+VA+SSGSACTSASLEPSYVLRA+G +ED+AH+SIRFGIGRFTTE
Sbjct: 351 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 410

Query: 418 AEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
            E+D  +Q  +H V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 411 EEVDYTVQKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 451


>NFS1_MOUSE (Q9Z1J3) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (m-Nfs1)
          Length = 451

 Score =  585 bits (1508), Expect = e-167
 Identities = 277/401 (69%), Positives = 340/401 (84%)

Query: 58  RPLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGA 117
           RPLY+DVQAT+P+DPRVLDAMLP+ ++ YGNPHSRTH YGWES+ A+E AR QVASLIGA
Sbjct: 51  RPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 110

Query: 118 SPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYL 177
            P+EI+FTSGATESNNI+IKGV  FY+ +K+H++TTQTEHKCVLDSCR L+ EGF VTYL
Sbjct: 111 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 170

Query: 178 PVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQA 237
           PV+  G++DL++L AAI+PDT LVSVM VNNEIGV QP+ EI +IC    V FHTDAAQA
Sbjct: 171 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIRQICSSRKVYFHTDAAQA 230

Query: 238 LGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTI 297
           +GK+P+DV    + LMS+SGHK+YGPKGVGA+Y+RRRPR+RVE   +GGGQERG+RSGT+
Sbjct: 231 VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 290

Query: 298 PTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNL 357
           PTPLVVG+GAACE+A +EMEYD KRIS L ERL+  I + L  VV+NG  ++ Y G +NL
Sbjct: 291 PTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQKIMKNLPDVVMNGDPKQHYPGCINL 350

Query: 358 SFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTE 417
           SFAYVEGESLLM LK+VA+SSGSACTSASLEPSYV RA+G +ED+AH+SIRFGIGRFTTE
Sbjct: 351 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVFRAIGTDEDLAHSSIRFGIGRFTTE 410

Query: 418 AEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
            E+D   +  +H V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 411 EEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 451


>NFS1_DROME (Q9VKD3) Probable cysteine desulfurase, mitochondrial
           precursor (EC 2.8.1.7)
          Length = 462

 Score =  582 bits (1499), Expect = e-166
 Identities = 286/456 (62%), Positives = 354/456 (76%), Gaps = 1/456 (0%)

Query: 4   KLLASTLRHHLTKP-TQLLRRHFLSTAAAAAAAAEPQQHHDESAGLTMKGVKIAGRPLYL 62
           ++ A  LR   T     ++R    S+  AA  AA  ++  +      +K  +  GRPLYL
Sbjct: 7   RIQAQVLRSRATNALANVVRHEATSSRTAAKPAATSKEFRERQVRFNIKNEQTEGRPLYL 66

Query: 63  DVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGASPKEI 122
           D QAT+P+DPRVLDAMLP+  + YGNPHSRTH YGWE++ AVE AR QVA+LIGA PKEI
Sbjct: 67  DAQATTPMDPRVLDAMLPYLTNFYGNPHSRTHAYGWETESAVEKAREQVATLIGADPKEI 126

Query: 123 VFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESD 182
           +FTSGATESNNI++KGV  FY  KKRHVITTQTEHKCVLDSCR L+ EGF VTYLPV ++
Sbjct: 127 IFTSGATESNNIAVKGVARFYGTKKRHVITTQTEHKCVLDSCRALENEGFKVTYLPVLAN 186

Query: 183 GLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQALGKVP 242
           GL+DL++L   I  +T LVS+M VNNEIGV QP++EIG++C+   V FHTDAAQA+GKVP
Sbjct: 187 GLIDLQQLEETITSETSLVSIMTVNNEIGVRQPVDEIGKLCRSRRVFFHTDAAQAVGKVP 246

Query: 243 VDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIPTPLV 302
           +DV   N+ LMS+SGHKIYGPKGVGALY+RRRPR+R+EP  +GGGQERG+RSGT+P PL 
Sbjct: 247 LDVNAMNIDLMSISGHKIYGPKGVGALYVRRRPRVRLEPIQSGGGQERGLRSGTVPAPLA 306

Query: 303 VGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLSFAYV 362
           VG+GAA E++++EM+YD+K +  L  RLL+ I   L  V+ NG  +  Y G LNLSFAYV
Sbjct: 307 VGLGAAAELSLREMDYDKKWVDFLSNRLLDRISSALPHVIRNGDAKATYNGCLNLSFAYV 366

Query: 363 EGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEAEIDR 422
           EGESLLM LK+VA+SSGSACTSASLEPSYVLRA+G +ED+AH+SIRFGIGRFTT  E+D 
Sbjct: 367 EGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTVEEVDY 426

Query: 423 AIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
                +  VE+LREMSPL+EMV+EGI++K IQW+QH
Sbjct: 427 TADKCIKHVERLREMSPLWEMVQEGIDLKTIQWSQH 462


>ISCS_RICCN (Q92HP1) Cysteine desulfurase (EC 2.8.1.7)
          Length = 410

 Score =  545 bits (1404), Expect = e-155
 Identities = 247/400 (61%), Positives = 331/400 (82%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGAS 118
           P+Y+D QAT+P+DPRV++AMLP++ +++GNPHSR+H +GWE++ AVE AR+ VA LIGA 
Sbjct: 11  PIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEARSMVAKLIGAD 70

Query: 119 PKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLP 178
            KEI+FTSGATESNN++IKG+  FY  KK H+IT  +EHKCVLD+CRHL+QEG  +TYLP
Sbjct: 71  TKEIIFTSGATESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLEQEGIKITYLP 130

Query: 179 VESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQAL 238
           ++ +G++DLE L+ AI   T LVSVM VNNEIGV QP++EIG+IC+E  V FH+D AQ  
Sbjct: 131 IKPNGIIDLETLKNAITDQTMLVSVMVVNNEIGVVQPLKEIGKICREKGVFFHSDIAQGF 190

Query: 239 GKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIP 298
           GK+P+DV  +N+ L S+SGHKIYGPKG+GALY+R++PR+RV P +NGGGQERG+RSGT+P
Sbjct: 191 GKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQERGMRSGTLP 250

Query: 299 TPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLS 358
           TPL+VG+G A E+A  EME D K ++ L +R LN I +++  V +NG   +RY GNLNLS
Sbjct: 251 TPLIVGLGMAAEIAYSEMEKDTKHVNYLFDRFLNNIHKRISEVYLNGDKNQRYKGNLNLS 310

Query: 359 FAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEA 418
           FA VEGES+++ +K++AVSSGSACTSASLEPSYVLR++G+ E++AHT+IRFGIGRFTTE 
Sbjct: 311 FAGVEGESMILAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIRFGIGRFTTEQ 370

Query: 419 EIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           E+D A+ L   +++KLRE+SPL+EM++EGI++K I+WA H
Sbjct: 371 EVDYAVNLICSKIDKLRELSPLWEMMQEGIDLKKIKWAVH 410


>ISCS_RICPR (Q9ZD60) Cysteine desulfurase (EC 2.8.1.7) (NifS protein
           homolog)
          Length = 410

 Score =  544 bits (1402), Expect = e-154
 Identities = 246/400 (61%), Positives = 333/400 (82%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGAS 118
           P+Y+D Q+T+P+DPRV++AMLP++ +++GNPHSR+H +GWE++ AVENAR+ VA +IGA 
Sbjct: 11  PIYMDYQSTTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVENARSMVAKVIGAD 70

Query: 119 PKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLP 178
            KEI+FTSGATESNN+ IKG+  FY  KK+H+IT  +EHKCVL++CRHL+QEG  +TYLP
Sbjct: 71  SKEIIFTSGATESNNLVIKGIAKFYGNKKKHIITLVSEHKCVLNACRHLEQEGIKITYLP 130

Query: 179 VESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQAL 238
           ++S+G++DLE L+ AI   T LVSVMAVNNEIGV QP++EIG+IC+E NV FH+D AQ  
Sbjct: 131 IKSNGIIDLETLKNAITDQTLLVSVMAVNNEIGVIQPLKEIGKICRERNVFFHSDIAQGF 190

Query: 239 GKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIP 298
           GK+P++V + N+ L S+SGHKIYGPKG+GALY+R++PR+RV P +NGGGQERG+RSGT+P
Sbjct: 191 GKIPINVNECNIDLASISGHKIYGPKGIGALYIRKKPRVRVTPLINGGGQERGMRSGTLP 250

Query: 299 TPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLS 358
           TPL+VG+G A E+A  EME D + ++ L +R LN I  K+  V +NG  ++RY GNLNLS
Sbjct: 251 TPLIVGLGIASEIAYNEMEKDTQHVNYLFDRFLNNIHSKISEVYLNGDKDQRYKGNLNLS 310

Query: 359 FAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEA 418
           FA VEGES+++ +K++AVSSGSACTSASLEPSYVLR++G+ E++AHTSIRFGIGRFTTE 
Sbjct: 311 FAGVEGESIILAIKDLAVSSGSACTSASLEPSYVLRSIGISEELAHTSIRFGIGRFTTEQ 370

Query: 419 EIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           EID A+ L   +++KLR +SPL+EM++EG+++K I+W  H
Sbjct: 371 EIDYAVNLVCSKIDKLRRLSPLWEMMQEGVDLKKIRWTAH 410


>NFS1_CANAL (P87185) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (tRNA splicing protein SPL1)
          Length = 488

 Score =  528 bits (1361), Expect = e-150
 Identities = 258/438 (58%), Positives = 332/438 (74%), Gaps = 4/438 (0%)

Query: 24  HFLSTAAAAAAAAEPQQHHDESAGLTMKGVKIAG-RPLYLDVQATSPVDPRVLDAMLPFY 82
           H  +    A  + EP +    S     +   + G RP+YLDVQAT+PVDPRVLD ML FY
Sbjct: 52  HVSTPVDTAKISIEPPEGSSISLKSASRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFY 111

Query: 83  ISRYGNPHSRTHFYGWESDEAVENARAQVASLIGASPKEIVFTSGATESNNISIKGVLHF 142
              YGNPHS TH YGWE+D+ VE AR  +A +I A PKEI+FTSGATE+NN++IKGV  F
Sbjct: 112 TGLYGNPHSSTHAYGWETDKEVEKARTYIADVINADPKEIIFTSGATETNNMAIKGVPRF 171

Query: 143 YKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRAAIRPDTGLVS 202
           YK+ K+H+ITTQTEHKCVLDS RH+Q EGF+VTYLPV S+GL++L+ L+ AIR DT LVS
Sbjct: 172 YKKTKKHIITTQTEHKCVLDSARHMQDEGFEVTYLPVSSEGLINLDDLKKAIRKDTVLVS 231

Query: 203 VMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQALGKVPVDVEKWNVSLMSLSGHKIYG 262
           +MAVNNEIGV QP++EIG+IC+E  V FHTDAAQA GK+P+DV + N+ L+S+S HKIYG
Sbjct: 232 IMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYG 291

Query: 263 PKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIPTPLVVGMGAACEVAMKEMEYDEKR 322
           PKG+GA Y+RRRPR+R++P + GGGQERG+RSGT+  PLV G G A  +  +E  +D++ 
Sbjct: 292 PKGIGACYVRRRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQESSFDKRH 351

Query: 323 ISALQERLLNGIREKLDGVVVNG--SMERRYAGNLNLSFAYVEGESLLMGLKEVAVSSGS 380
           I  L  +L NG+   +     NG    + +Y G +N+SFAY+EGESLLM LK++A+SSGS
Sbjct: 352 IEKLSSKLKNGLL-SIPSTQFNGCNDAKSQYPGCVNVSFAYIEGESLLMALKDIALSSGS 410

Query: 381 ACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEAEIDRAIQLTVHQVEKLREMSPL 440
           ACTSASLEPSYVL ALG ++ +AH+SIRFGIGRFTTEAE+D  IQ    +V+ LR+MSPL
Sbjct: 411 ACTSASLEPSYVLHALGADDALAHSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPL 470

Query: 441 YEMVKEGINIKDIQWAQH 458
           +EMV+EGI++  I+W+ H
Sbjct: 471 WEMVQEGIDLNTIEWSGH 488


>NFS1_ASHGO (O60028) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (tRNA splicing protein SPL1)
          Length = 490

 Score =  528 bits (1360), Expect = e-149
 Identities = 248/401 (61%), Positives = 323/401 (79%), Gaps = 1/401 (0%)

Query: 58  RPLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGA 117
           RP+YLD+QAT+P DPRV+D ML FY   YGNPHS TH YGWE+ + VE AR  VA +I A
Sbjct: 91  RPIYLDMQATTPTDPRVVDTMLKFYTGLYGNPHSNTHSYGWETSQEVEKARKNVADVIKA 150

Query: 118 SPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYL 177
            PKEI+FTSGATESNN+++KGV  FYK++K H+ITT+TEHKCVL++ R ++ EGFDVT+L
Sbjct: 151 DPKEIIFTSGATESNNMALKGVARFYKKRKNHIITTRTEHKCVLEAARSMKDEGFDVTFL 210

Query: 178 PVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQA 237
            V  DGLV LE+L  AIRP+T LVSVM+VNNEIGV QP++EIG IC+   V FH+DAAQA
Sbjct: 211 NVNEDGLVSLEELEQAIRPETSLVSVMSVNNEIGVVQPIKEIGAICRRHKVFFHSDAAQA 270

Query: 238 LGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTI 297
            GK+P+DV++ N+ L+S+S HKIYGPKG+GALY+RRRPR+R+EP ++GGGQERG RSGT+
Sbjct: 271 YGKIPIDVDEMNIDLLSISSHKIYGPKGIGALYVRRRPRVRMEPLLSGGGQERGFRSGTL 330

Query: 298 PTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNL 357
           P PLVVG+G A ++ ++E EYD   +  L +RLL G+   ++   +NGS + RY G +N+
Sbjct: 331 PPPLVVGLGHAAKLMVEEYEYDSAHVRRLSDRLLKGLL-SIEQTTLNGSADHRYPGCVNV 389

Query: 358 SFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTE 417
           SFA+VEGESLLM L+++A+SSGSACTSASLEPSYVL A+G ++ +AH+SIRFGIGRFTTE
Sbjct: 390 SFAFVEGESLLMALRDIALSSGSACTSASLEPSYVLHAIGRDDALAHSSIRFGIGRFTTE 449

Query: 418 AEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           AE+D  I+    +VE LRE+SPL+EM K+GI++  I+W+ H
Sbjct: 450 AEVDYVIKAITERVEFLRELSPLWEMYKDGIDLNTIEWSGH 490


>NFS1_SCHPO (O74351) Probable cysteine desulfurase, mitochondrial
           precursor (EC 2.8.1.7)
          Length = 498

 Score =  526 bits (1355), Expect = e-149
 Identities = 253/415 (60%), Positives = 332/415 (79%), Gaps = 5/415 (1%)

Query: 44  ESAGLTMKGVKIAGRPLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEA 103
           ++A  T+ G+     P+Y+D QATSP+D RVLD+MLPF+   YGNPHSRTH YGWE+++A
Sbjct: 89  QTAASTVTGL----HPIYMDFQATSPLDYRVLDSMLPFFTGIYGNPHSRTHAYGWEAEKA 144

Query: 104 VENARAQVASLIGASPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDS 163
           VENAR ++AS+I A P+EI+FTSGATESNN  +KGV  FYK +K+H+++ QTEHKCVLDS
Sbjct: 145 VENARQEIASVINADPREIIFTSGATESNNAILKGVARFYKSRKKHLVSVQTEHKCVLDS 204

Query: 164 CRHLQQEGFDVTYLPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRIC 223
            R LQ+EGF+VT+LPV+++GL++L++LR AIRPDT  VSVMAVNNEIGV QP+EEIG+IC
Sbjct: 205 LRALQEEGFEVTFLPVQTNGLINLDELRDAIRPDTVCVSVMAVNNEIGVCQPLEEIGKIC 264

Query: 224 KEFNVPFHTDAAQALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQM 283
           ++  V FH+DAAQ  GK+ +DV + N+ LMS+S HKIYGPKG+GA Y+RRRPR+R+EP +
Sbjct: 265 RQKKVFFHSDAAQGYGKIDIDVNRMNIDLMSISAHKIYGPKGIGAAYVRRRPRVRLEPLI 324

Query: 284 NGGGQERGIRSGTIPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVV 343
           +GGGQERG+RSGT+    VVG G A  +  +EM+YD   IS L +RL++G+   +    +
Sbjct: 325 SGGGQERGLRSGTLAPSQVVGFGTAARICKEEMKYDYAHISKLSQRLIDGLL-AIPYTSL 383

Query: 344 NGSMERRYAGNLNLSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMA 403
           NG  + RY G +N+SF YVEGESLLMGLK +A+SSGSACTSASLEPSYVLRA+G  ++ A
Sbjct: 384 NGDPKSRYPGCVNISFNYVEGESLLMGLKNIALSSGSACTSASLEPSYVLRAIGQSDENA 443

Query: 404 HTSIRFGIGRFTTEAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           H+SIRFGIGRFTTEAEID AI+    QV  LR MSPL+++V+EG+++  I+W+QH
Sbjct: 444 HSSIRFGIGRFTTEAEIDYAIENVSRQVSFLRNMSPLWDLVQEGVDLSTIEWSQH 498


>NFS1_CANMA (P87187) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (tRNA splicing protein SPL1)
          Length = 484

 Score =  526 bits (1354), Expect = e-149
 Identities = 255/428 (59%), Positives = 328/428 (76%), Gaps = 8/428 (1%)

Query: 38  PQQHHDESAGLTMKGVK-----IAGRPLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSR 92
           P  H  + + +++K           RP+YLDVQAT+PVDPRVLD ML FY   YGNPHS 
Sbjct: 58  PSVHPPDGSSISLKSATRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSS 117

Query: 93  THFYGWESDEAVENARAQVASLIGASPKEIVFTSGATESNNISIKGVLHFYKEKKRHVIT 152
           TH YGWE+D+ VE AR  +A +I A PKEI+FTSGATE+NN++IKGV  FYK+ K+H+IT
Sbjct: 118 THAYGWETDKEVEKARGYIADVINADPKEIIFTSGATETNNMAIKGVPRFYKKTKKHIIT 177

Query: 153 TQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGV 212
           TQTEHKCVLDS RH+Q EGFD+TYLPV S+GL++L+ L+ AIR DT LVSVMAVNNEIGV
Sbjct: 178 TQTEHKCVLDSARHMQDEGFDITYLPVNSEGLINLDDLKKAIRKDTVLVSVMAVNNEIGV 237

Query: 213 FQPMEEIGRICKEFNVPFHTDAAQALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLR 272
            QP++EIG+IC+E  + FHTDAAQA GK+P+DV + N+ L+S+S HKIYGPKG+GA Y+R
Sbjct: 238 IQPLKEIGQICRENKIFFHTDAAQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVR 297

Query: 273 RRPRIRVEPQMNGGGQERGIRSGTIPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLN 332
           RRPR+R++P + GGGQERG+RSGT+  PLV G G A  +  +E  +D+K I  L  +L N
Sbjct: 298 RRPRVRLDPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQESAFDKKHIEKLSTKLKN 357

Query: 333 GIREKLDGVVVNG--SMERRYAGNLNLSFAYVEGESLLMGLKEVAVSSGSACTSASLEPS 390
           G+   +     NG  +   +Y G +N+SFAY+EGESLLM LK++A+SSGSACTSASLEPS
Sbjct: 358 GLL-SIPSTQFNGCNNPTYQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPS 416

Query: 391 YVLRALGVEEDMAHTSIRFGIGRFTTEAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINI 450
           YVL ALG ++ +AH+SIRFGIGRFTTEAE+D  IQ    +V+ LR+MSPL+EMV+ GI++
Sbjct: 417 YVLHALGADDALAHSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQGGIDL 476

Query: 451 KDIQWAQH 458
             I+W+ H
Sbjct: 477 NSIEWSGH 484


>NFS1_YEAST (P25374) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (tRNA splicing protein SPL1)
          Length = 497

 Score =  516 bits (1328), Expect = e-146
 Identities = 244/401 (60%), Positives = 320/401 (78%), Gaps = 1/401 (0%)

Query: 58  RPLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGA 117
           RP+YLD+QAT+P DPRVLD ML FY   YGNPHS TH YGWE++ AVENARA VA +I A
Sbjct: 98  RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNTAVENARAHVAKMINA 157

Query: 118 SPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYL 177
            PKEI+FTSGATESNN+ +KGV  FYK+ K+H+ITT+TEHKCVL++ R + +EGF+VT+L
Sbjct: 158 DPKEIIFTSGATESNNMVLKGVPRFYKKTKKHIITTRTEHKCVLEAARAMMKEGFEVTFL 217

Query: 178 PVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQA 237
            V+  GL+DL++L  AIRPDT LVSVMAVNNEIGV QP++EIG IC++  + FHTDAAQA
Sbjct: 218 NVDDQGLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICRKNKIYFHTDAAQA 277

Query: 238 LGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTI 297
            GK+ +DV + N+ L+S+S HKIYGPKG+GA+Y+RRRPR+R+EP ++GGGQERG+RSGT+
Sbjct: 278 YGKIHIDVNEMNIDLLSISSHKIYGPKGIGAIYVRRRPRVRLEPLLSGGGQERGLRSGTL 337

Query: 298 PTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNL 357
             PLV G G A  +  KE + D+  I  L ++L+ G+    +   +NGS + RY G +N+
Sbjct: 338 APPLVAGFGEAARLMKKEFDNDQAHIKRLSDKLVKGLL-SAEHTTLNGSPDHRYPGCVNV 396

Query: 358 SFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTE 417
           SFAYVEGESLLM L+++A+SSGSACTSASLEPSYVL ALG ++ +AH+SIRFGIGRF+TE
Sbjct: 397 SFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFSTE 456

Query: 418 AEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
            E+D  ++    +V+ LRE+SPL+EMV+EGI++  I+W+ H
Sbjct: 457 EEVDYVVKAVSDRVKFLRELSPLWEMVQEGIDLNSIKWSGH 497


>ISCS_VIBPA (Q87S28) Cysteine desulfurase (EC 2.8.1.7)
          Length = 404

 Score =  501 bits (1290), Expect = e-141
 Identities = 242/402 (60%), Positives = 317/402 (78%), Gaps = 3/402 (0%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYI--SRYGNPHSRTHFYGWESDEAVENARAQVASLIG 116
           P+YLD  AT PVDPRV + M+ +      +GNP SR+H YGW+++EAV+ AR Q+A L+ 
Sbjct: 4   PIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIADLLN 63

Query: 117 ASPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTY 176
           A P+EIVFTSGATES+N++IKG  HFY +K +HVIT +TEHK VLD CR L++EGF+VTY
Sbjct: 64  ADPREIVFTSGATESDNLAIKGAAHFYSKKGKHVITCKTEHKAVLDPCRQLEREGFEVTY 123

Query: 177 LPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQ 236
           L  ES+GL+DLEKL+AA+R DT LVS+M VNNEIGV Q +  IG +C+E  + FH DAAQ
Sbjct: 124 LEPESNGLIDLEKLKAAMRDDTVLVSIMHVNNEIGVIQDIAAIGELCRERKIVFHVDAAQ 183

Query: 237 ALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGT 296
           + GK+P+DV++  V L+SLS HK+YGPKG+GALY+RR+PRIR+E QM+GGG ERG RSGT
Sbjct: 184 SAGKLPIDVQEMKVDLISLSAHKVYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFRSGT 243

Query: 297 IPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLN 356
           + T  +VGMG A  +A +EM+ D     AL+ RLLNG+++ L+ V VNG +E+R   NLN
Sbjct: 244 LATHQIVGMGEAFRIAKEEMQKDYDHALALRNRLLNGVKD-LEAVTVNGDLEQRVPQNLN 302

Query: 357 LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTT 416
           +SFA+VEGESLLM LK++AVSSGSACTSASLEPSYVLRALG+++++AH+S+RF  GRFTT
Sbjct: 303 ISFAFVEGESLLMSLKDLAVSSGSACTSASLEPSYVLRALGMDDELAHSSVRFSFGRFTT 362

Query: 417 EAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           E EID AI+     V KLR+MSPL++M KEG+++  ++WA H
Sbjct: 363 EEEIDYAIEQIRVAVTKLRDMSPLWDMYKEGVDLSTVEWAHH 404


>ISCS_VIBVU (Q8DEY7) Cysteine desulfurase (EC 2.8.1.7)
          Length = 404

 Score =  495 bits (1275), Expect = e-140
 Identities = 240/402 (59%), Positives = 316/402 (77%), Gaps = 3/402 (0%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYI--SRYGNPHSRTHFYGWESDEAVENARAQVASLIG 116
           P+YLD  AT PVDPRV + M+ +      +GNP SR+H YGW+++EAV+ AR Q+A L+ 
Sbjct: 4   PIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAELLN 63

Query: 117 ASPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTY 176
           A P+EIVFTSGATES+N++IKG  HFY ++ +HVIT++TEHK VLD+CR L++EGF+VTY
Sbjct: 64  ADPREIVFTSGATESDNLAIKGAAHFYSKQGKHVITSKTEHKAVLDTCRQLEREGFEVTY 123

Query: 177 LPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQ 236
           L  ES+GL+ L KL AA+R DT LVS+M VNNEIGV Q +E IG +C+   + FH DAAQ
Sbjct: 124 LEPESNGLISLSKLEAAMRDDTVLVSIMHVNNEIGVIQDIEAIGELCRSRKIIFHVDAAQ 183

Query: 237 ALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGT 296
           + GKV +DV+K  V L+SLS HKIYGPKG+GALY+RR+PRIR+E QM+GGG ERG RSGT
Sbjct: 184 SAGKVAIDVQKLKVDLISLSAHKIYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFRSGT 243

Query: 297 IPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLN 356
           +PT  +VGMG A  +A  +ME D +   AL+ RLL+G+++ ++ V +NG +++R   NLN
Sbjct: 244 LPTHQIVGMGEAFRIAKLDMEKDYQHALALRNRLLDGVKD-MEAVTINGDLDQRVPHNLN 302

Query: 357 LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTT 416
           +SFA+VEGESLLM LK++AVSSGSACTSASLEPSYVLRALG+ +++AH+SIRF  GRFTT
Sbjct: 303 ISFAFVEGESLLMSLKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTT 362

Query: 417 EAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           E EID AI+     VEKLR+MSPL++M K+GI++  ++WA H
Sbjct: 363 EEEIDYAIEQIRVAVEKLRDMSPLWDMYKDGIDLNTVEWAHH 404


>ISCS_VIBCH (Q9KTY2) Cysteine desulfurase (EC 2.8.1.7) (NifS protein
           homolog)
          Length = 404

 Score =  489 bits (1259), Expect = e-138
 Identities = 238/402 (59%), Positives = 316/402 (78%), Gaps = 3/402 (0%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYI--SRYGNPHSRTHFYGWESDEAVENARAQVASLIG 116
           P+YLD  AT PVDPRV + M+ +      +GNP SR+H YGW+++EAV+ AR Q+A+L+ 
Sbjct: 4   PIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLN 63

Query: 117 ASPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTY 176
           A P+EIVFTSGATES+N++IKGV HFY ++ +H+IT++TEHK VLD+ R L++EGF+VTY
Sbjct: 64  ADPREIVFTSGATESDNLAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTY 123

Query: 177 LPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQ 236
           L  ES+GLVDL KL AA+R DT LVS+M VNNEIGV Q +  IG +C+   V FH DAAQ
Sbjct: 124 LDPESNGLVDLAKLEAAMRDDTILVSIMHVNNEIGVVQDIAAIGELCRSRKVVFHVDAAQ 183

Query: 237 ALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGT 296
           + GKV +DV++  V L+SLS HK YGPKG+GALY+RR+PRIR+E QM+GGG ERG RSGT
Sbjct: 184 SAGKVAIDVQEMKVDLISLSAHKAYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFRSGT 243

Query: 297 IPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLN 356
           +PT  +VGMG A  +A +E++ D      L+ RLL+GI++ ++ V +NG +++R   NLN
Sbjct: 244 LPTHQIVGMGEAFRIAKEELQQDYDHALKLRNRLLDGIKD-MEAVTINGDLDQRVPHNLN 302

Query: 357 LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTT 416
           +SFA+VEGESLLM LK++AVSSGSACTSASLEPSYVLRALG+ +++AH+SIRF  GRFTT
Sbjct: 303 VSFAFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTT 362

Query: 417 EAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           EAEID AI+L    V+KLR MSPL++M K+G+++  ++WA H
Sbjct: 363 EAEIDYAIELIRVAVDKLRAMSPLWDMYKDGVDLNTVEWAHH 404


>ISCS_NEIMB (Q9JYY0) Cysteine desulfurase (EC 2.8.1.7) (NifS protein
           homolog)
          Length = 404

 Score =  481 bits (1237), Expect = e-135
 Identities = 236/400 (59%), Positives = 304/400 (76%), Gaps = 1/400 (0%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGAS 118
           P+YLD  AT PVD RV + M+P+    +GNP S +H +GWE++EAVE ARA +A+LI A 
Sbjct: 6   PVYLDYAATPPVDKRVAEKMIPYLTETFGNPASNSHSFGWEAEEAVEKARADIAALINAD 65

Query: 119 PKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLP 178
            KEIVFTSGATESNN++IKG  HFYK K  H+IT +TEHK VLD+ R L+++G++VTYL 
Sbjct: 66  SKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKTEHKAVLDTMRELERQGYEVTYLD 125

Query: 179 VESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQAL 238
           V+ +GLVDL+ L+AAIR DT LVSVM VNNEIGV Q +  IG IC+E  + FH DAAQA 
Sbjct: 126 VQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVVQDIPAIGEICRERKIIFHVDAAQAC 185

Query: 239 GKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIP 298
           GKVPVDVE   V L+S+SGHK+YGPKG+GALY+RR+PR+R+E QM+GGG ERG RSGT+P
Sbjct: 186 GKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFRSGTLP 245

Query: 299 TPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLS 358
           T  +VGMG A  +A +E+  D      L++  L GI E ++ V +NG +E R   NLN+S
Sbjct: 246 THQIVGMGEAFRIAKEELAQDTAHYLKLRDIFLKGI-EGIEEVYINGDLEHRVPNNLNVS 304

Query: 359 FAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEA 418
           F +VEGESL+M +KE+AVSSGSACTSASLEPSYVLRALG  +++AH+S+R   GR TTE 
Sbjct: 305 FNFVEGESLIMAVKELAVSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRMTTEE 364

Query: 419 EIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           E+  A +L   ++ KLRE+SPL+EM K+GI++  I+WA H
Sbjct: 365 EVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIEWAAH 404


>ISCS_SALTY (Q8ZN40) Cysteine desulfurase (EC 2.8.1.7) (ThiI
           transpersulfidase) (NifS protein homolog)
          Length = 404

 Score =  479 bits (1233), Expect = e-135
 Identities = 228/402 (56%), Positives = 314/402 (77%), Gaps = 3/402 (0%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYI--SRYGNPHSRTHFYGWESDEAVENARAQVASLIG 116
           P+YLD  AT+PVDPRV + M+ F      +GNP SR+H +GW+++EAV+ AR Q+A L+G
Sbjct: 4   PIYLDYSATTPVDPRVAEKMMQFLTLDGTFGNPASRSHRFGWQAEEAVDIARNQIAELVG 63

Query: 117 ASPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTY 176
           A P+EIVFTSGATES+N++IKG  +FY++K +H+IT++TEHK VLD+CR L++EGF+VTY
Sbjct: 64  ADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTY 123

Query: 177 LPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQ 236
           L  + +G++DL +L AA+R DT LVS+M VNNEIGV Q +  IG +C+   + +H DA Q
Sbjct: 124 LAPQRNGIIDLNELEAAMRDDTILVSIMHVNNEIGVVQDIATIGEMCRARGIIYHVDATQ 183

Query: 237 ALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGT 296
           ++GK+P+D+ +  V LMS SGHKIYGPKG+GALY+RR+PRIR+E QM+GGG ERG+RSGT
Sbjct: 184 SVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYVRRKPRIRIEAQMHGGGHERGMRSGT 243

Query: 297 IPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLN 356
           +P   +VGMG A  +A +EME +  R+  L+ RL NGI++ ++ V +NG +E+     LN
Sbjct: 244 LPVHQIVGMGEAYRIAKEEMETEMARLRGLRNRLWNGIKD-IEEVYLNGDLEQGAPNILN 302

Query: 357 LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTT 416
           +SF YVEGESL+M LK++AVSSGSACTSASLEPSYVLRALG+ +++AH+SIRF +GRFTT
Sbjct: 303 VSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGMNDELAHSSIRFSLGRFTT 362

Query: 417 EAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           E EID  I L    + +LR++SPL+EM K+G+++  I+WA H
Sbjct: 363 EEEIDYTIDLVRKSIGRLRDLSPLWEMYKQGVDLNSIEWAHH 404


>ISCS_NEIMA (Q9JTX0) Cysteine desulfurase (EC 2.8.1.7) (NifS protein
           homolog)
          Length = 404

 Score =  478 bits (1231), Expect = e-134
 Identities = 233/400 (58%), Positives = 306/400 (76%), Gaps = 1/400 (0%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGAS 118
           P+YLD  AT+ VD RV + M+P+    +GNP S +H +GWE++EAVE ARA +A+LI A 
Sbjct: 6   PVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFGWEAEEAVEKARADIAALINAD 65

Query: 119 PKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLP 178
           PKEIVFTSGATES+N++IKG  +FYK K +H+IT +TEHK VLD+ R L+++GF+VTYL 
Sbjct: 66  PKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKTEHKAVLDTMRELERQGFEVTYLG 125

Query: 179 VESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQAL 238
           V+ +GL+DLE+L+AAIR DT LVSVM  NNEIGV Q +  IG IC+E  + FH DAAQA 
Sbjct: 126 VQENGLIDLEELKAAIRDDTILVSVMWANNEIGVVQDIPAIGEICRERKIVFHVDAAQAC 185

Query: 239 GKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIP 298
           GKVPVDVE   + L+S+S HK+YGPKG+GALY+RR+PR+R+E QM+GGG ERG RSGT+P
Sbjct: 186 GKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYVRRKPRVRLEAQMHGGGHERGFRSGTLP 245

Query: 299 TPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLS 358
           T  +VGMG A  +A +E+E D      L++  L GI E ++ V +NG +E R   NLN+S
Sbjct: 246 THQIVGMGEAFRIAKEELEQDMAHYRKLRDIFLKGI-EGIEEVYINGDLEHRVPNNLNVS 304

Query: 359 FAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEA 418
           F +VEGESL+M +KE+AVSSGSACTSASLEPSYVLRALG  +++AH+S+R   GR TTE 
Sbjct: 305 FNFVEGESLIMAVKELAVSSGSACTSASLEPSYVLRALGRNDELAHSSLRITFGRMTTEE 364

Query: 419 EIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           E+  A +L   ++ KLRE+SPL+EM K+GI++  I+WA H
Sbjct: 365 EVQFAAELIKSKIGKLRELSPLWEMFKDGIDLNSIEWAAH 404


>ISCS_SALTI (Q8Z4N0) Cysteine desulfurase (EC 2.8.1.7) (ThiI
           transpersulfidase) (NifS protein homolog)
          Length = 404

 Score =  478 bits (1230), Expect = e-134
 Identities = 227/402 (56%), Positives = 314/402 (77%), Gaps = 3/402 (0%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYI--SRYGNPHSRTHFYGWESDEAVENARAQVASLIG 116
           P+YLD  AT+PVDPRV + M+ F      +GNP SR+H +GW+++EAV+ AR Q+A L+G
Sbjct: 4   PIYLDYSATTPVDPRVAEKMMQFLTLDGTFGNPASRSHRFGWQAEEAVDIARNQIAELVG 63

Query: 117 ASPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTY 176
           A P+EIVFTSGATES+N++IKG  +FY++K +H+IT++TEHK VLD+CR L++EGF+VTY
Sbjct: 64  ADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTY 123

Query: 177 LPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQ 236
           L  + +G++DL +L AA+R DT LVS+M VNNEIGV Q +  IG +C+   + +H DA Q
Sbjct: 124 LAPQRNGIIDLNELEAAMRDDTILVSIMHVNNEIGVVQDIATIGEMCRARGIIYHVDATQ 183

Query: 237 ALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGT 296
           ++GK+P+D+ +  V LMS SGHKIYGPKG+GALY+RR+PRIR+E QM+GGG ERG+RSGT
Sbjct: 184 SVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYVRRKPRIRIEAQMHGGGHERGMRSGT 243

Query: 297 IPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLN 356
           +P   +VGMG A  +A +EME +  R+  L+ RL NGI++ ++ V +NG +E+     LN
Sbjct: 244 LPVHQIVGMGEAYRIAKEEMETEMARLRGLRNRLWNGIKD-IEEVYLNGDLEQGAPNILN 302

Query: 357 LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTT 416
           +SF YVEGESL+M LK++AVSSGSACTSA+LEPSYVLRALG+ +++AH+SIRF +GRFTT
Sbjct: 303 VSFNYVEGESLIMALKDLAVSSGSACTSATLEPSYVLRALGMNDELAHSSIRFSLGRFTT 362

Query: 417 EAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           E EID  I L    + +LR++SPL+EM K+G+++  I+WA H
Sbjct: 363 EEEIDYTIDLVRKSIGRLRDLSPLWEMYKQGVDLNSIEWAHH 404


>ISCS_HAEIN (Q57337) Cysteine desulfurase (EC 2.8.1.7) (NifS protein
           homolog)
          Length = 404

 Score =  478 bits (1230), Expect = e-134
 Identities = 232/402 (57%), Positives = 310/402 (76%), Gaps = 3/402 (0%)

Query: 59  PLYLDVQATSPVDPRVLDAMLPFYI--SRYGNPHSRTHFYGWESDEAVENARAQVASLIG 116
           P+YLD  AT PVD RV   M+ F      +GNP SR+H +GW+++EAV+ AR Q+A LIG
Sbjct: 4   PIYLDYAATCPVDERVAKKMMAFLTIDGTFGNPASRSHKFGWQAEEAVDIARNQIADLIG 63

Query: 117 ASPKEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTY 176
           A  +EIVFTSGATES+N++IKG  HFY+ K +H+IT +TEHK VLD+CR L++EGF+VTY
Sbjct: 64  ADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITCKTEHKAVLDTCRQLEREGFEVTY 123

Query: 177 LPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQ 236
           L  E+DGL+DLEK +AA+RPDT L S+M  NNEIGV Q ++ IG +C+     FH DA Q
Sbjct: 124 LSPEADGLIDLEKFKAALRPDTILASIMHANNEIGVLQDIKAIGELCRANKTIFHVDATQ 183

Query: 237 ALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGT 296
           ++GKV +++E+  V LMS+S HK+YGPKGVGALY+RR+PR+R+E  ++GGG ERG+RSGT
Sbjct: 184 SVGKVEINLEELAVDLMSMSSHKLYGPKGVGALYVRRKPRVRLEAIIHGGGHERGMRSGT 243

Query: 297 IPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLN 356
           +P   +VGMG A  +A +EM  +  R+ AL++RL NG+++ ++   VNGSME R   NLN
Sbjct: 244 LPVHQIVGMGEAYRIAKEEMASEMPRLKALRDRLYNGLKD-IEETYVNGSMEHRLDSNLN 302

Query: 357 LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTT 416
           +SF YVEGESL+M L+++AVSSGSACTSASLEPSYVLRALG+ +++AH+SIRF +GR+TT
Sbjct: 303 ISFNYVEGESLMMALRDIAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYTT 362

Query: 417 EAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
           E EID  I L    VEKLR +SPL++M KEGI++  I+W+ H
Sbjct: 363 EEEIDYTINLMKGAVEKLRALSPLWDMFKEGIDLNTIEWSAH 404


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.318    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,844,947
Number of Sequences: 164201
Number of extensions: 2271010
Number of successful extensions: 6650
Number of sequences better than 10.0: 190
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 55
Number of HSP's that attempted gapping in prelim test: 6285
Number of HSP's gapped (non-prelim): 197
length of query: 458
length of database: 59,974,054
effective HSP length: 114
effective length of query: 344
effective length of database: 41,255,140
effective search space: 14191768160
effective search space used: 14191768160
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 67 (30.4 bits)