BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0126.13
(168 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MYS3_HUMAN (Q92794) MYST histone acetyltransferase 3 (Runt-relat... 39 0.004
DYNA_CHICK (P35458) Dynactin 1 (150 kDa dynein-associated polype... 38 0.010
ARFK_ARATH (Q9ZPY6) Auxin response factor 11 36 0.036
UN89_CAEEL (O01761) Muscle M-line assembly protein unc-89 (Uncoo... 35 0.081
NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa neur... 35 0.081
PAN1_YEAST (P32521) PAN1 protein 35 0.11
IF2_XYLFT (Q87EV4) Translation initiation factor IF-2 34 0.14
ZPW1_MOUSE (Q6IR42) Zinc finger CW-type PWWP domain protein 1 ho... 34 0.18
NFM_RABIT (P54938) Neurofilament triplet M protein (160 kDa neur... 34 0.18
CENE_HUMAN (Q02224) Centromeric protein E (CENP-E protein) 34 0.18
ADDB_RAT (Q05764) Beta adducin (Erythrocyte adducin beta subunit... 34 0.18
TRIA_HUMAN (Q15643) Thyroid receptor interacting protein 11 (TRI... 33 0.24
TONB_NEIMB (P57004) TonB protein 33 0.24
TONB_NEIMA (P57003) TonB protein 33 0.24
NFH_RAT (P16884) Neurofilament triplet H protein (200 kDa neurof... 33 0.31
CORO_SCHPO (O13923) Coronin-like protein crn1 33 0.31
RAI1_MOUSE (Q61818) Retinoic acid induced protein 1 33 0.40
IF2_CHRVO (Q7NY13) Translation initiation factor IF-2 33 0.40
TBP_HUMAN (P20226) TATA-box binding protein (TATA-box factor) (T... 32 0.52
HOC1_YEAST (P47124) Putative glycosyltransferase HOC1 (EC 2.4.-.... 32 0.52
>MYS3_HUMAN (Q92794) MYST histone acetyltransferase 3 (Runt-related
transcription factor binding protein 2) (Monocytic
leukemia zinc finger protein) (Zinc finger protein 220)
Length = 2004
Score = 39.3 bits (90), Expect = 0.004
Identities = 27/94 (28%), Positives = 47/94 (49%), Gaps = 5/94 (5%)
Query: 6 IQISNELVNQLVDDPVPKKKIRRNRRKVAKETEKPQSKVTGKPENAAAPGWPVQSPLFLP 65
IQ S E V D P+P++ R+ ++ E E+ + G+ E+AA+ P SP
Sbjct: 1204 IQESEETVEPKEDMPLPEE--RKEEEEMQAEAEEAEE---GEEEDAASSEVPAASPADSS 1258
Query: 66 AKPPVQPADPEIDGIRSVLRESEKVLESLQKQEE 99
P + +PE++ R SE+ +S ++Q+E
Sbjct: 1259 NSPETETKEPEVEEEEEKPRVSEEQRQSEEEQQE 1292
>DYNA_CHICK (P35458) Dynactin 1 (150 kDa dynein-associated
polypeptide) (DP-150) (DAP-150) (p150-glued)
Length = 1224
Score = 38.1 bits (87), Expect = 0.010
Identities = 40/149 (26%), Positives = 64/149 (42%), Gaps = 26/149 (17%)
Query: 22 PKKKIRRNRRKVAKETEKPQSKVTGKPENAAA---------PGWPVQSPLFLPAKP---- 68
PKK R + T P S G +A+A P P Q+PL P P
Sbjct: 136 PKKTTARRPKPTRTPTSAPSSGTAGPSGSASASGGEMSSSEPSTPAQTPLVAPVIPSPSL 195
Query: 69 --PVQPADP----EIDGIRSVLRESEKVLESL---QKQEENMLQEVTQKAKDLHD-KEYK 118
PV P P E + +RS +R+ E+ LE+L + +++ L+E+ + L +E+K
Sbjct: 196 TSPVAPMVPSPTKEEENLRSQVRDLEEKLETLKIKRNEDKAKLKELEKYKIQLEQVQEWK 255
Query: 119 LPNPKPEPCIAERLATLSCYKEHIKDPLK 147
+ + + RL K+ KD L+
Sbjct: 256 SKMQEQQADLQRRLKEA---KKEAKDALE 281
>ARFK_ARATH (Q9ZPY6) Auxin response factor 11
Length = 601
Score = 36.2 bits (82), Expect = 0.036
Identities = 29/95 (30%), Positives = 46/95 (47%), Gaps = 10/95 (10%)
Query: 32 KVAKETEKPQSKVTGKPENAAAPGWPVQSPLFLPAKPPVQ-------PADPEIDGIRSVL 84
K ET++ +++T +PE + + PL PAKP V +D G SVL
Sbjct: 83 KAEHETDEVYAQITLQPEEDQSEPTSLDPPLVEPAKPTVDSFVKILTASDTSTHGGFSVL 142
Query: 85 RE-SEKVLESLQKQEENMLQEVTQKAKDLHDKEYK 118
R+ + + L SL + QE+ A+DLH E++
Sbjct: 143 RKHATECLPSLDMTQPTPTQELV--ARDLHGYEWR 175
>UN89_CAEEL (O01761) Muscle M-line assembly protein unc-89
(Uncoordinated protein 89)
Length = 6632
Score = 35.0 bits (79), Expect = 0.081
Identities = 31/147 (21%), Positives = 59/147 (40%), Gaps = 15/147 (10%)
Query: 3 EFTIQISNELVNQLVDDPV--PKKKIRRNRRKVAKETEKPQSKVTGKPENAAAPGWPVQS 60
E + E + VD+ P KK + + + +E + P K + P P +
Sbjct: 1521 ESRTSVKKEKTPEKVDEKPKSPTKKDKSPEKSITEEIKSPVKKEKSPEKVEEKPASPTKK 1580
Query: 61 PLFLPAKPPVQPADPEIDGIRSVLRESEKVLESLQKQEENMLQEVTQKAKDLHDKEYKLP 120
K P +PA P ++SE ++S K+E++ + V ++ K +K +
Sbjct: 1581 E-----KSPEKPASPT--------KKSENEVKSPTKKEKSPEKSVVEELKSPKEKSPEKA 1627
Query: 121 NPKPEPCIAERLATLSCYKEHIKDPLK 147
+ KP+ + + E +K P K
Sbjct: 1628 DDKPKSPTKKEKSPEKSATEDVKSPTK 1654
>NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa
neurofilament protein) (Neurofilament medium
polypeptide) (NF-M) (Neurofilament 3)
Length = 915
Score = 35.0 bits (79), Expect = 0.081
Identities = 35/138 (25%), Positives = 59/138 (42%), Gaps = 14/138 (10%)
Query: 20 PVPKKKIRRNRRKVAKET---EKPQSKVTGKPENAAAPGWPVQSPLFLPAKPPVQPADPE 76
PVPK + + ++ EK +S V P +SP+ AK PV P P
Sbjct: 628 PVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPV-PKSPV 686
Query: 77 ID-------GIRSVLRESEKVLESLQKQEENMLQEVTQKAKDLHDKEYKLPNPKPEPCIA 129
+ G E EK ++ K+E+ +++ +K KD+ +K+ K +P E +A
Sbjct: 687 EEAKSKAEVGKGEQKEEEEKEVKEAPKEEK--VEKKEEKPKDVPEKK-KAESPVKEEAVA 743
Query: 130 ERLATLSCYKEHIKDPLK 147
E + K H++ K
Sbjct: 744 EVVTITKSVKVHLEKETK 761
>PAN1_YEAST (P32521) PAN1 protein
Length = 1480
Score = 34.7 bits (78), Expect = 0.11
Identities = 27/90 (30%), Positives = 41/90 (45%), Gaps = 6/90 (6%)
Query: 32 KVAKETEKPQSKVTGKPENAAAPGWPVQSPLFLPAKPPVQPADPEIDGIRSVLRESEKVL 91
+VA+ T+ QS +P P PVQ + PVQP P +++V ++
Sbjct: 1081 EVAQPTQPVQSTQPVQPTQPVQPTQPVQPTQPVQPTQPVQPTQP----VQNVYNAKQESD 1136
Query: 92 ESLQKQEENMLQEVTQ--KAKDLHDKEYKL 119
+ + EE LQE + K K DKE +L
Sbjct: 1137 DEDEDDEEKRLQEELKRLKLKKKADKEKRL 1166
>IF2_XYLFT (Q87EV4) Translation initiation factor IF-2
Length = 892
Score = 34.3 bits (77), Expect = 0.14
Identities = 31/114 (27%), Positives = 54/114 (47%), Gaps = 5/114 (4%)
Query: 24 KKIRRNRRKVAKETEKP-QSKVTGKPENAAAPGW--PVQSPLFLPAKPPVQPADPE-IDG 79
KKI NRR++ + T ++K T E + P +S P KPP++ AD E ++
Sbjct: 70 KKITLNRRRLQEVTVNAGRNKTTVNVEVRQKRTYVKPPESEYHTPTKPPIELADAERVEI 129
Query: 80 IRSVLRESEKVLESLQKQEENMLQEV-TQKAKDLHDKEYKLPNPKPEPCIAERL 132
+R + ++ L Q+ E Q V Q+ K +++ +L K E + E +
Sbjct: 130 LRKLEESRQRNLAEQQRLAEVDRQRVEEQERKRREEEQAELERQKTESRVVEEI 183
>ZPW1_MOUSE (Q6IR42) Zinc finger CW-type PWWP domain protein 1
homolog
Length = 630
Score = 33.9 bits (76), Expect = 0.18
Identities = 31/101 (30%), Positives = 48/101 (46%), Gaps = 7/101 (6%)
Query: 5 TIQISNELVNQLVDDPVPKKKIR--RNRRKVAKETEKPQSKVTGK-PENAAAPG-WPVQS 60
T+ E N +D PVPKKK + N+ +E+ + K+ K P +A G P+
Sbjct: 511 TLGGKEEQGNSDLDHPVPKKKFKAPENKTSATNLSEEKEIKIVSKCPTPSAQHGACPLGK 570
Query: 61 PLFLPAKPPVQPAD--PEIDGIRSVLRESEKVLESLQKQEE 99
+P PP Q A P D S L + E+++E + + EE
Sbjct: 571 EGLVPHMPPTQEAASFPPDDDCSSDL-DLEQLMEDIGEPEE 610
>NFM_RABIT (P54938) Neurofilament triplet M protein (160 kDa
neurofilament protein) (Neurofilament medium
polypeptide) (NF-M) (Fragment)
Length = 644
Score = 33.9 bits (76), Expect = 0.18
Identities = 32/114 (28%), Positives = 48/114 (42%), Gaps = 16/114 (14%)
Query: 23 KKKIRRNRRKVAKETEKPQSKVTGKPENAAAPGWPVQSPLFLPAKPPVQPADPEID---G 79
KK ++ AKE ++KV GKPE A +P K PV+ P+ + G
Sbjct: 372 KKTEEKSEEVAAKEEPVTEAKV-GKPEKAKSP----------VPKSPVEEVKPKAEATAG 420
Query: 80 IRSVLRESEKVLESLQKQEENMLQEVTQKAKDLHDKEYKLPNPKPEPCIAERLA 133
E EKV E +K + +E +K + +K +P K E + E A
Sbjct: 421 KGEQKEEEEKVEEEKKKAAKESPKE--EKVEKKEEKPKDVPKKKAESPVKEEAA 472
>CENE_HUMAN (Q02224) Centromeric protein E (CENP-E protein)
Length = 2663
Score = 33.9 bits (76), Expect = 0.18
Identities = 21/67 (31%), Positives = 38/67 (56%), Gaps = 1/67 (1%)
Query: 77 IDGIRSVLRESEKVLESLQKQEENMLQEVTQKAKDLHDKEYKLPNPKPEPCIAERLATLS 136
I+ +R L E E + ++QKQ E + ++ K +++++KE +L N K + E + L
Sbjct: 1493 INELRVNLSEKETEISTIQKQLEAINDKLQNKIQEIYEKEEQL-NIKQISEVQENVNELK 1551
Query: 137 CYKEHIK 143
+KEH K
Sbjct: 1552 QFKEHRK 1558
>ADDB_RAT (Q05764) Beta adducin (Erythrocyte adducin beta subunit)
(Adducin 63)
Length = 725
Score = 33.9 bits (76), Expect = 0.18
Identities = 29/106 (27%), Positives = 48/106 (44%), Gaps = 11/106 (10%)
Query: 14 NQLVDDPVPKKKIRRNRRKVAKETEKPQSKVTGKPENA--AAPGWPVQSPLFLPAK-PPV 70
+QL D + + K R+K+ +E E + T +P + + P PVQSP K P V
Sbjct: 558 SQLTDQELEEYKKEVERKKLEQEQEGEKDAATEEPGSPVKSTPASPVQSPTRAGTKSPAV 617
Query: 71 QPADPEIDGIRSVLRESEKVLESLQ--------KQEENMLQEVTQK 108
P+ D ++ + E+ E + K+EE ++EV K
Sbjct: 618 SPSKASEDAKKTEVSEANTEPEPEKPEGVVVNGKEEEPCVEEVLSK 663
>TRIA_HUMAN (Q15643) Thyroid receptor interacting protein 11
(TRIP-11) (Golgi-associated microtubule-binding protein
210) (GMAP-210) (Trip230) (Clonal evolution related gene
on chromosome 14)
Length = 1979
Score = 33.5 bits (75), Expect = 0.24
Identities = 18/71 (25%), Positives = 39/71 (54%), Gaps = 4/71 (5%)
Query: 80 IRSVLRESEKVLESLQKQEENMLQEVTQKAKDLHDKEYKLPNPKPEPCIAE---RLATLS 136
+R + E ++ L S+ K+E N LQE ++ ++ + + +PK + E ++ L+
Sbjct: 844 LRQTIEEKDRSLGSM-KEENNHLQEELERLREEQSRTAPVADPKTLDSVTELASEVSQLN 902
Query: 137 CYKEHIKDPLK 147
KEH+++ +K
Sbjct: 903 TIKEHLEEEIK 913
>TONB_NEIMB (P57004) TonB protein
Length = 280
Score = 33.5 bits (75), Expect = 0.24
Identities = 27/86 (31%), Positives = 39/86 (44%), Gaps = 7/86 (8%)
Query: 46 GKPENAAAPGWPVQSPLFLPAKPPVQPADPEIDGIRSVLRESEKVLESLQKQEENMLQEV 105
G PE A AP P P+ P K PV+P P + + ++K +Q+ +E E
Sbjct: 54 GAPEGAGAPAAPEPQPVPEPPK-PVEPPKPVLKPV-----VTKKADADIQQPKEEPKPEE 107
Query: 106 TQKAKDLHDKEYKLPNPKPEPCIAER 131
K ++ E K P KP P AE+
Sbjct: 108 KPKPEEKPKPEPK-PEAKPVPKPAEK 132
>TONB_NEIMA (P57003) TonB protein
Length = 280
Score = 33.5 bits (75), Expect = 0.24
Identities = 27/86 (31%), Positives = 39/86 (44%), Gaps = 7/86 (8%)
Query: 46 GKPENAAAPGWPVQSPLFLPAKPPVQPADPEIDGIRSVLRESEKVLESLQKQEENMLQEV 105
G PE A AP P P+ P K PV+P P + + ++K +Q+ +E E
Sbjct: 54 GAPEGAGAPAAPEPQPVPEPPK-PVEPPKPVLKPV-----VTKKADADIQQPKEEPKPEE 107
Query: 106 TQKAKDLHDKEYKLPNPKPEPCIAER 131
K ++ E K P KP P AE+
Sbjct: 108 KPKPEEKPKPEPK-PEAKPVPKPAEK 132
>NFH_RAT (P16884) Neurofilament triplet H protein (200 kDa
neurofilament protein) (Neurofilament heavy polypeptide)
(NF-H) (Fragment)
Length = 831
Score = 33.1 bits (74), Expect = 0.31
Identities = 28/105 (26%), Positives = 41/105 (38%), Gaps = 3/105 (2%)
Query: 22 PKKKIRRNRRKVAKETEKPQSKVTGKPENAAAPGWPVQSPLFLPAKPPVQPADPEIDGIR 81
P KK+ + +TE +SK P+ A P + PL K A E +
Sbjct: 649 PPKKVEEEKTPATPKTEVKESKKDEAPKEAQKPKAEEKEPLTEKPKDSPGEAKKEEAKEK 708
Query: 82 SVLRESEKVLESLQKQEENMLQEVTQKAKDLHDKEYKLPNPKPEP 126
E+ L +EE +E KA+D KE P+ K +P
Sbjct: 709 KAAAPEEETPAKLGVKEEAKPKE---KAEDAKAKEPSKPSEKEKP 750
>CORO_SCHPO (O13923) Coronin-like protein crn1
Length = 601
Score = 33.1 bits (74), Expect = 0.31
Identities = 31/126 (24%), Positives = 49/126 (38%), Gaps = 21/126 (16%)
Query: 5 TIQISNELVNQLVDDPVPKKKIRRNRRKVAK-ETEKPQSKVTGKPENAAAPGWPVQSPLF 63
T+ S V + V VP + + K ET KP+++ KP+ +A P + P
Sbjct: 394 TLYESKGTVEKAVSATVPSAGAQVQKHNEEKVETPKPEAQPVSKPKESAEEQKPSKEPEV 453
Query: 64 LPAKPPVQPADPEIDGIRSVLRESEKVLESLQKQEENMLQE---VTQKAKDLHDKEYKLP 120
P P + KV E +K++E+ Q+ VTQ ++ E P
Sbjct: 454 KPTTP-----------------SASKVEEPSKKRDEDNHQKEETVTQPKREKTPVEKSFP 496
Query: 121 NPKPEP 126
P P
Sbjct: 497 KPASSP 502
>RAI1_MOUSE (Q61818) Retinoic acid induced protein 1
Length = 1889
Score = 32.7 bits (73), Expect = 0.40
Identities = 26/78 (33%), Positives = 30/78 (38%), Gaps = 4/78 (5%)
Query: 16 LVDDPVPKKKIRRNRR---KVAKETEKPQSKVTGKPEN-AAAPGWPVQSPLFLPAKPPVQ 71
L P P K+ +R K K KP K P N AA P SP+ K P
Sbjct: 1057 LTTTPTPPDKLGGKQRAAFKSGKRVGKPSPKAASSPSNPAALPVASDSSPMGSKTKEPDS 1116
Query: 72 PADPEIDGIRSVLRESEK 89
P+ P D VLR K
Sbjct: 1117 PSMPGKDQRSMVLRSRTK 1134
>IF2_CHRVO (Q7NY13) Translation initiation factor IF-2
Length = 964
Score = 32.7 bits (73), Expect = 0.40
Identities = 28/112 (25%), Positives = 45/112 (40%), Gaps = 17/112 (15%)
Query: 20 PVPKKKIRRNRRKVAKETEKPQSKVTGKPENAAAPGWPVQSPLFLPAKPPVQPADPEIDG 79
P PK + + + K KP+ KV KPE P KP PA +
Sbjct: 120 PEPKPEPKVEAKPEPKPEPKPEPKVEAKPEPKPEP------------KPAAAPAPRTVAS 167
Query: 80 IRS----VLRES-EKVLESLQKQEENMLQEVTQKAKDLHDKEYKLPNPKPEP 126
I S RE+ EK + + ++E +++E ++ + + P PEP
Sbjct: 168 ILSPEEIAAREAEEKRQAAFRARQEALMREKIEREERRQAAKLAASQPAPEP 219
>TBP_HUMAN (P20226) TATA-box binding protein (TATA-box factor) (TATA
binding factor) (TATA sequence-binding protein)
(Transcription initiation factor TFIID TBP subunit)
Length = 339
Score = 32.3 bits (72), Expect = 0.52
Identities = 17/77 (22%), Positives = 37/77 (47%), Gaps = 1/77 (1%)
Query: 40 PQSKVTGKPENAAAPGWPVQSPLFLPAKPPVQPADPEIDGIRSVLRESEKVLESLQKQEE 99
P ++ P+ A PG P+ SP+ +P + P + S+L E ++ + Q+Q++
Sbjct: 9 PYAQGLASPQGAMTPGIPIFSPM-MPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQ 67
Query: 100 NMLQEVTQKAKDLHDKE 116
Q+ Q+ + ++
Sbjct: 68 QQQQQQQQQQQQQQQQQ 84
>HOC1_YEAST (P47124) Putative glycosyltransferase HOC1 (EC 2.4.-.-)
(Mannan polymerase II complex HOC1 subunit) (M-Pol II
subunit Hoc1p)
Length = 395
Score = 32.3 bits (72), Expect = 0.52
Identities = 16/63 (25%), Positives = 31/63 (48%)
Query: 83 VLRESEKVLESLQKQEENMLQEVTQKAKDLHDKEYKLPNPKPEPCIAERLATLSCYKEHI 142
+++ E + + ++ Q+E ++ ++ K L K L PE + ER+A Y H+
Sbjct: 68 LVQHFESLAQEIRHQQEVQAKQFDKQRKILEKKIQDLKQTPPEATLRERIAMTFPYDSHV 127
Query: 143 KDP 145
K P
Sbjct: 128 KFP 130
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.313 0.132 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,674,812
Number of Sequences: 164201
Number of extensions: 883498
Number of successful extensions: 4068
Number of sequences better than 10.0: 177
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 134
Number of HSP's that attempted gapping in prelim test: 3952
Number of HSP's gapped (non-prelim): 248
length of query: 168
length of database: 59,974,054
effective HSP length: 102
effective length of query: 66
effective length of database: 43,225,552
effective search space: 2852886432
effective search space used: 2852886432
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0126.13