BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0113.14
         (584 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310...   107  9e-23
YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein ...    38  0.064
VPP4_HUMAN (Q9HBG4) Vacuolar proton translocating ATPase 116 kDa...    33  1.6
TRM1_METAC (Q8TGX6) N(2),N(2)-dimethylguanosine tRNA methyltrans...    33  2.1
TRM1_METMA (Q8PU28) N(2),N(2)-dimethylguanosine tRNA methyltrans...    33  2.7
EBSB_ENTFA (P36921) Cell wall enzyme ebsB                              33  2.7
LON2_MAIZE (P93648) Lon protease homolog 2, mitochondrial precur...    31  7.8

>M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310
           (ORF154)
          Length = 154

 Score =  107 bits (267), Expect = 9e-23
 Identities = 54/142 (38%), Positives = 79/142 (55%), Gaps = 2/142 (1%)

Query: 25  LPTYAMAILKFPKQFCHQIASQIARFWWRG-NNDRGIHWKNWASLMFSKAE-GGMGFKDF 82
           LP YAM+  +  K  C ++ S +  FWW    N R I W  W  L  SK + GG+GF+D 
Sbjct: 3   LPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDL 62

Query: 83  AVLNQACLAKQAWRILQEPNAFWASVLKGIYFPNENFLHAKKKRQASWAWNSIIHGRELI 142
              NQA LAKQ++RI+ +P+   + +L+  YFP+ + +      + S+AW SIIHGREL+
Sbjct: 63  GWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELL 122

Query: 143 LNQGRWSIEDGKSVNLLGHRWL 164
                 +I DG    +   RW+
Sbjct: 123 SRGLLRTIGDGIHTKVWLDRWI 144


>YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein (ORF
            2)
          Length = 1308

 Score = 38.1 bits (87), Expect = 0.064
 Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 2/72 (2%)

Query: 255  SEPSKECWRSI*SARIPHKIKVFLWKIMHEALPTKVNLRRRHCVSETSCPICEAPREDAA 314
            +E  +  WR+  S+ +P       WK++H AL T   L  R   S  +CP C    E   
Sbjct: 1115 NEGERPQWRAFYSSLVPRPTGDLSWKVLHGALSTGEYL-ARFTDSPAACPFC-GKGESVF 1172

Query: 315  HVFLHCPWLRPV 326
            H +  C  L+P+
Sbjct: 1173 HAYFTCARLQPL 1184


>VPP4_HUMAN (Q9HBG4) Vacuolar proton translocating ATPase 116 kDa
           subunit a isoform 4 (V-ATPase 116-kDa isoform a4)
           (Vacuolar proton translocating ATPase 116 kDa subunit a
           kidney isoform)
          Length = 840

 Score = 33.5 bits (75), Expect = 1.6
 Identities = 21/56 (37%), Positives = 27/56 (47%), Gaps = 9/56 (16%)

Query: 132 WNSIIHGRELILNQGRWSIEDG--------KSVNLLGHRWLACNERLREGPLDTTV 179
           WN+  HGR LIL  G +SI  G        KS+N+ G  W +     R G  +T V
Sbjct: 442 WNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSW-SVQPMFRNGTWNTHV 496


>TRM1_METAC (Q8TGX6) N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase)
          Length = 388

 Score = 33.1 bits (74), Expect = 2.1
 Identities = 24/72 (33%), Positives = 36/72 (49%), Gaps = 2/72 (2%)

Query: 391 PVLCLNLAVEASKEFLLETTTEFLPIYGGHVNRGIRHWTRPPLGFLKCNTDAGFLRVGNK 450
           P   L+ A  A++  L  T T+  P+ G H+N GIR +   PL   + +++ G LRV   
Sbjct: 143 PAPYLDAAATAAQGMLSVTATDTAPLCGAHLNSGIRKYAAVPLN-TEYHSEMG-LRVLLG 200

Query: 451 AAGGYIFKDDKG 462
           A      K +KG
Sbjct: 201 ACARECAKHEKG 212


>TRM1_METMA (Q8PU28) N(2),N(2)-dimethylguanosine tRNA
           methyltransferase (EC 2.1.1.32)
           (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
           2,2-dimethylguanosine-26 methyltransferase)
           (tRNA(m(2,2)G26)dimethyltransferase)
          Length = 388

 Score = 32.7 bits (73), Expect = 2.7
 Identities = 24/74 (32%), Positives = 38/74 (50%), Gaps = 4/74 (5%)

Query: 389 PNPVLCLNLAVEASKEFLLETTTEFLPIYGGHVNRGIRHWTRPPLGFLKCNTDAGFLRVG 448
           P+P L  + A  ++   L  T T+  P+ G H+N GIR +   PL   + +++ G LRV 
Sbjct: 143 PSPYL--DAAASSAYSMLSVTATDTAPLCGAHLNSGIRKYASVPLN-TEYHSEMG-LRVL 198

Query: 449 NKAAGGYIFKDDKG 462
             A    + K +KG
Sbjct: 199 LGACARELAKHEKG 212


>EBSB_ENTFA (P36921) Cell wall enzyme ebsB
          Length = 135

 Score = 32.7 bits (73), Expect = 2.7
 Identities = 31/137 (22%), Positives = 60/137 (43%), Gaps = 13/137 (9%)

Query: 436 LKCNTDAGFLRVGNKAAGGYIFKDDKGCFV*GNHRQLHAASALA----AEALILREAVFA 491
           L+   DA       ++ GG ++  D+      + +QLH    +     AE  +L EA+  
Sbjct: 2   LRIYVDAATKGNPGESGGGIVYLTDQ------SRQQLHVPLGIVSNHEAEFKVLIEALKK 55

Query: 492 AM--NLNMDRVVFEADNLGLIEACREQREKGE-ILAIVKDIRKCKLDFPNWIFSWTRREG 548
           A+    N   V+  +D+  +++   +   K E     + + ++ + +FP  +  W     
Sbjct: 56  AIANEDNQQTVLLHSDSKIVVQTIEKNYAKNEKYQPYLAEYQQLEKNFPLLLIEWLPESQ 115

Query: 549 NECAHVLARLALCRSIP 565
           N+ A +LAR AL +  P
Sbjct: 116 NKAADMLARQALQKFYP 132


>LON2_MAIZE (P93648) Lon protease homolog 2, mitochondrial precursor
           (EC 3.4.21.-)
          Length = 964

 Score = 31.2 bits (69), Expect = 7.8
 Identities = 22/59 (37%), Positives = 27/59 (45%), Gaps = 1/59 (1%)

Query: 153 GKSVNLLGHRWLACNERLREGPLDTTVSSLMEN-QTRTWSISKITSVLSLPDLGRVLQT 210
           G  V LLGHR L   E + E PL   V  L E    +   + K TS   +  L  VL+T
Sbjct: 180 GDHVVLLGHRRLRITEMVEEDPLTVKVDHLKEKPYNKDDDVMKATSFEVISTLREVLRT 238


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.324    0.138    0.455 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,177,578
Number of Sequences: 164201
Number of extensions: 3075063
Number of successful extensions: 7086
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 7083
Number of HSP's gapped (non-prelim): 7
length of query: 584
length of database: 59,974,054
effective HSP length: 116
effective length of query: 468
effective length of database: 40,926,738
effective search space: 19153713384
effective search space used: 19153713384
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 69 (31.2 bits)