BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0106.15
(120 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YJL2_YEAST (P47025) Hypothetical 80.0 kDa Trp-Asp repeats contai... 30 0.74
KGUA_PSEAE (Q9HTM2) Guanylate kinase (EC 2.7.4.8) (GMP kinase) 30 0.74
MQO4_STAEP (Q8CMY4) Probable malate:quinone oxidoreductase 4 (EC... 30 0.97
MQO2_STAEP (Q8CQ96) Probable malate:quinone oxidoreductase 2 (EC... 30 1.3
MFT1_YEAST (P33441) Mitochondrial fusion target protein 30 1.3
KTR3_YEAST (P38130) Probable mannosyltransferase KTR3 (EC 2.4.1.... 30 1.3
NEP1_THEVU (Q60053) Neopullulanase 1 precursor (EC 3.2.1.135) (A... 29 1.6
Y745_HELPY (O25441) Hypothetical pseudouridine synthase HP0745 (... 29 2.2
ETF1_VACCV (P04308) Early transcription factor 70 kDa subunit (V... 29 2.2
ETF1_VACCT (Q9JFA3) Early transcription factor 70 kDa subunit (V... 29 2.2
ETF1_VACCC (P20634) Early transcription factor 70 kDa subunit (V... 29 2.2
SNGP_RAT (Q9QUH6) Ras GTPase-activating protein SynGAP (Synaptic... 28 2.8
SNGP_HUMAN (Q96PV0) Ras GTPase-activating protein SynGAP (Synapt... 28 2.8
ETF1_VARV (P33056) Early transcription factor 70 kDa subunit (VE... 28 2.8
ETF1_VACCA (O93120) Early transcription factor 70 kDa subunit (V... 28 2.8
YN65_ARCFU (O30305) Hypothetical UPF0051 protein AF2365 28 3.7
FTH3_SYNY3 (P73437) Cell division protein ftsH homolog 3 (EC 3.4... 28 3.7
VGLH_HHV7J (P52353) Glycoprotein H precursor 28 4.8
SPCB_HUMAN (P11277) Spectrin beta chain, erythrocyte (Beta-I spe... 28 4.8
SAMP_PIG (O19063) Serum amyloid P-component precursor (SAP) 28 4.8
>YJL2_YEAST (P47025) Hypothetical 80.0 kDa Trp-Asp repeats
containing protein in ASF1-CCT7 intergenic region
Length = 714
Score = 30.4 bits (67), Expect = 0.74
Identities = 14/48 (29%), Positives = 28/48 (58%)
Query: 5 LIEKVDNVAEGDHIKVDRVKEVDNEVDDMKEWGHILKEDEDLMNAIDD 52
L+ K+ N+ + + D +K++D+ +D ++E+G + E NA DD
Sbjct: 268 LLGKIANIEQNQLLLEDNLKQIDDRLDFLEEYGLEVIEANSDENAEDD 315
>KGUA_PSEAE (Q9HTM2) Guanylate kinase (EC 2.7.4.8) (GMP kinase)
Length = 203
Score = 30.4 bits (67), Expect = 0.74
Identities = 17/64 (26%), Positives = 34/64 (52%), Gaps = 1/64 (1%)
Query: 14 EGDHIKVDRVKEVDNEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQ 73
+ D + R++E +E+ E+ H++ D D +A+DD+ A+ Q+ Q A+
Sbjct: 138 DSDEVIERRMREAVSEMSHYVEYDHLVIND-DFAHALDDLKAIFRARQLRQDAQQQRHAE 196
Query: 74 LVGK 77
L+G+
Sbjct: 197 LLGR 200
>MQO4_STAEP (Q8CMY4) Probable malate:quinone oxidoreductase 4 (EC
1.1.99.16) (Malate dehydrogenase [acceptor] 4) (MQO 4)
Length = 499
Score = 30.0 bits (66), Expect = 0.97
Identities = 13/43 (30%), Positives = 27/43 (62%), Gaps = 2/43 (4%)
Query: 18 IKVDRVKEVDNEVDDMKE-WGHILKEDEDLMNAIDDMNALSHV 59
I +++ KE++ + + K+ WGH++K E + N + +N L H+
Sbjct: 75 IDIEKAKEINEQFEISKQFWGHLVKSGE-IQNPKEFINPLPHI 116
>MQO2_STAEP (Q8CQ96) Probable malate:quinone oxidoreductase 2 (EC
1.1.99.16) (Malate dehydrogenase [acceptor] 2) (MQO 2)
Length = 498
Score = 29.6 bits (65), Expect = 1.3
Identities = 13/43 (30%), Positives = 27/43 (62%), Gaps = 2/43 (4%)
Query: 18 IKVDRVKEVDNEVDDMKE-WGHILKEDEDLMNAIDDMNALSHV 59
I +++ KE++ + + K+ WGH++K ++ N D +N L H+
Sbjct: 75 IDIEKAKEINEQFEISKQFWGHLVKSG-NISNPRDFINPLPHI 116
>MFT1_YEAST (P33441) Mitochondrial fusion target protein
Length = 392
Score = 29.6 bits (65), Expect = 1.3
Identities = 16/41 (39%), Positives = 22/41 (53%)
Query: 6 IEKVDNVAEGDHIKVDRVKEVDNEVDDMKEWGHILKEDEDL 46
IE VD E DH D+ E + E DDM+ +KED ++
Sbjct: 288 IENVDEKEESDHEYDDQEDEENEEEDDMEVDVEDIKEDNEV 328
>KTR3_YEAST (P38130) Probable mannosyltransferase KTR3 (EC
2.4.1.131)
Length = 404
Score = 29.6 bits (65), Expect = 1.3
Identities = 22/71 (30%), Positives = 35/71 (48%), Gaps = 8/71 (11%)
Query: 9 VDNVAEGDHIK--VDRVKEVDNEVDDMKEWGHI---LKEDEDLMNAIDDMNALSHVVMKF 63
V VA D++ D+ + V + VDD K+ + L + DL N + ++ HV +F
Sbjct: 57 VSRVASKDYLMPFTDKSQGVIHPVDDGKKEKGVMVTLARNSDLWNLV---KSIRHVEDRF 113
Query: 64 QNRQVFDWAQL 74
NR +DW L
Sbjct: 114 NNRYHYDWVFL 124
>NEP1_THEVU (Q60053) Neopullulanase 1 precursor (EC 3.2.1.135)
(Alpha-amylase I) (TVA I)
Length = 666
Score = 29.3 bits (64), Expect = 1.6
Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
Query: 65 NRQVFDWAQLVGKNNDFV----VIMIRSDYGTLNGKESLTLGCEHGGKFKPYKGKFD 117
NR+ FDW+Q N+ +I IR+ Y L +TL + K Y G+FD
Sbjct: 548 NRRSFDWSQATPSNSAVALTQKLITIRNQYPALRTGSFMTLITDDTNKIYSY-GRFD 603
>Y745_HELPY (O25441) Hypothetical pseudouridine synthase HP0745 (EC
4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase)
Length = 327
Score = 28.9 bits (63), Expect = 2.2
Identities = 19/79 (24%), Positives = 37/79 (46%), Gaps = 9/79 (11%)
Query: 24 KEVDNEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQLVGKNNDFVV 83
KE+D E++ ++ EDEDL+ N + H + + + DW + KN +
Sbjct: 72 KELDLEIE-------VIFEDEDLLVLNKPPNLVVHKALSVKEPTLVDWLEF--KNYELSN 122
Query: 84 IMIRSDYGTLNGKESLTLG 102
+ ++ YG ++ + T G
Sbjct: 123 LGLKERYGIVHRLDKDTSG 141
>ETF1_VACCV (P04308) Early transcription factor 70 kDa subunit (VETF
small subunit)
Length = 637
Score = 28.9 bits (63), Expect = 2.2
Identities = 21/69 (30%), Positives = 34/69 (48%), Gaps = 6/69 (8%)
Query: 22 RVKEVDNEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQLVGKNNDF 81
R+K D+++ +K ++K ED N IDD+ H F + +VFD + L ND
Sbjct: 556 RIKYNDSKL--LKMVTSVIKNKEDARNYIDDI-VNGHF---FVSNKVFDKSLLYKYENDI 609
Query: 82 VVIMIRSDY 90
+ + R Y
Sbjct: 610 ITVPFRLSY 618
>ETF1_VACCT (Q9JFA3) Early transcription factor 70 kDa subunit (VETF
small subunit)
Length = 637
Score = 28.9 bits (63), Expect = 2.2
Identities = 21/69 (30%), Positives = 34/69 (48%), Gaps = 6/69 (8%)
Query: 22 RVKEVDNEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQLVGKNNDF 81
R+K D+++ +K ++K ED N IDD+ H F + +VFD + L ND
Sbjct: 556 RIKYNDSKL--LKMVTSVIKNKEDARNYIDDI-VNGHF---FVSNKVFDKSLLYKYENDI 609
Query: 82 VVIMIRSDY 90
+ + R Y
Sbjct: 610 ITVPFRLSY 618
>ETF1_VACCC (P20634) Early transcription factor 70 kDa subunit (VETF
small subunit)
Length = 637
Score = 28.9 bits (63), Expect = 2.2
Identities = 21/69 (30%), Positives = 34/69 (48%), Gaps = 6/69 (8%)
Query: 22 RVKEVDNEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQLVGKNNDF 81
R+K D+++ +K ++K ED N IDD+ H F + +VFD + L ND
Sbjct: 556 RIKYNDSKL--LKMVTSVIKNKEDARNYIDDI-VNGHF---FVSNKVFDKSLLYKYENDI 609
Query: 82 VVIMIRSDY 90
+ + R Y
Sbjct: 610 ITVPFRLSY 618
>SNGP_RAT (Q9QUH6) Ras GTPase-activating protein SynGAP (Synaptic
Ras-GTPase-activating protein 1) (Synaptic Ras-GAP 1)
(Neuronal RasGAP) (p135 SynGAP)
Length = 1293
Score = 28.5 bits (62), Expect = 2.8
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 2/53 (3%)
Query: 16 DHIKVDRVKEVDNEVDDMKEWGHI--LKEDEDLMNAIDDMNALSHVVMKFQNR 66
D ++DRVKE + E+ +KE H+ K +E + S ++M++Q R
Sbjct: 1173 DESRLDRVKEYEEEIHSLKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQAR 1225
>SNGP_HUMAN (Q96PV0) Ras GTPase-activating protein SynGAP (Synaptic
Ras-GTPase-activating protein 1) (Synaptic Ras-GAP 1)
(Neuronal RasGAP)
Length = 1328
Score = 28.5 bits (62), Expect = 2.8
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 2/53 (3%)
Query: 16 DHIKVDRVKEVDNEVDDMKEWGHI--LKEDEDLMNAIDDMNALSHVVMKFQNR 66
D ++DRVKE + E+ +KE H+ K +E + S ++M++Q R
Sbjct: 1173 DESRLDRVKEYEEEIHSLKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQAR 1225
>ETF1_VARV (P33056) Early transcription factor 70 kDa subunit (VETF
small subunit)
Length = 637
Score = 28.5 bits (62), Expect = 2.8
Identities = 19/63 (30%), Positives = 29/63 (45%), Gaps = 4/63 (6%)
Query: 28 NEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQLVGKNNDFVVIMIR 87
N+ +K ++K ED N IDD+ H F + +VFD + L ND + + R
Sbjct: 560 NDAKLLKMVTSVIKNKEDARNYIDDI-VNGHF---FVSNKVFDKSLLYKYENDIITVPFR 615
Query: 88 SDY 90
Y
Sbjct: 616 LSY 618
>ETF1_VACCA (O93120) Early transcription factor 70 kDa subunit (VETF
small subunit)
Length = 637
Score = 28.5 bits (62), Expect = 2.8
Identities = 19/63 (30%), Positives = 29/63 (45%), Gaps = 4/63 (6%)
Query: 28 NEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQLVGKNNDFVVIMIR 87
N+ +K ++K ED N IDD+ H F + +VFD + L ND + + R
Sbjct: 560 NDTKLLKMVTSVIKNKEDARNYIDDI-VNGHF---FVSNKVFDKSLLYKYENDIITVPFR 615
Query: 88 SDY 90
Y
Sbjct: 616 LSY 618
>YN65_ARCFU (O30305) Hypothetical UPF0051 protein AF2365
Length = 369
Score = 28.1 bits (61), Expect = 3.7
Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Query: 21 DRVKEVDNEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQ 73
+R+KEV E+D K G L+ED+D + + +K Q + +DW +
Sbjct: 17 ERLKEVGIELDKSKRSGVYLQEDQDAKTFSSFFEGVEVMSIK-QAMEKYDWVK 68
>FTH3_SYNY3 (P73437) Cell division protein ftsH homolog 3 (EC
3.4.24.-)
Length = 628
Score = 28.1 bits (61), Expect = 3.7
Identities = 15/35 (42%), Positives = 21/35 (59%), Gaps = 5/35 (14%)
Query: 21 DRVKEVDNEVDDMKEWGH-----ILKEDEDLMNAI 50
D KE+D EV ++ E GH IL+ + DL+ AI
Sbjct: 564 DTAKEIDLEVKEIVEQGHNQALAILEHNRDLLEAI 598
>VGLH_HHV7J (P52353) Glycoprotein H precursor
Length = 690
Score = 27.7 bits (60), Expect = 4.8
Identities = 17/87 (19%), Positives = 47/87 (53%), Gaps = 7/87 (8%)
Query: 6 IEKVDNVAEGDHIKVDRVKEVDNEVDDMKEWGHILKEDEDLMNAID------DMNALSHV 59
I+ + N++ D + + + +++D + ++ +I+ +D+ L+ ID D+N +H
Sbjct: 584 IKPIYNISSHDCVFCESLVVEYDDIDGIIQFVYIM-DDKQLLKLIDPDTNFIDVNPRTHY 642
Query: 60 VMKFQNRQVFDWAQLVGKNNDFVVIMI 86
++ +N VF+ L K++ ++++
Sbjct: 643 LLFLRNGSVFEITALDLKSSQVSIMLV 669
>SPCB_HUMAN (P11277) Spectrin beta chain, erythrocyte (Beta-I
spectrin)
Length = 2137
Score = 27.7 bits (60), Expect = 4.8
Identities = 18/75 (24%), Positives = 41/75 (54%), Gaps = 8/75 (10%)
Query: 1 MSHV--LIEKVDNVAEGDHIKVDRVKEVDN---EVDDMKEWGHILKEDEDLMNAIDDMNA 55
MSH+ E++ N+A G ++++ K + E+D+ + W +KE E + +++D
Sbjct: 612 MSHLEQCFEELSNMAAGARTQLEQSKRLWKFFWEMDEAESW---IKEKEQIYSSLDYGKD 668
Query: 56 LSHVVMKFQNRQVFD 70
L+ V++ + + F+
Sbjct: 669 LTSVLILQRKHKAFE 683
>SAMP_PIG (O19063) Serum amyloid P-component precursor (SAP)
Length = 224
Score = 27.7 bits (60), Expect = 4.8
Identities = 17/67 (25%), Positives = 29/67 (42%), Gaps = 9/67 (13%)
Query: 21 DRVKEVDNEVDDMKEWGHILKEDEDLMNAIDDMNALSHVVMKFQNRQVFDWAQLVGKNND 80
D+ + E+ D+ WG +L +E L + + F + + DW L + N
Sbjct: 164 DKTQSFVGEIGDLYMWGSVLSPNEI---------RLVYQGLSFPHPTILDWQALNYEMNG 214
Query: 81 FVVIMIR 87
+VVI R
Sbjct: 215 YVVIKPR 221
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.139 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,923,349
Number of Sequences: 164201
Number of extensions: 583012
Number of successful extensions: 1670
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 1660
Number of HSP's gapped (non-prelim): 36
length of query: 120
length of database: 59,974,054
effective HSP length: 96
effective length of query: 24
effective length of database: 44,210,758
effective search space: 1061058192
effective search space used: 1061058192
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0106.15