BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.3
(186 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YCF2_PINTH (P41653) Protein ycf2 33 0.29
LIGE_PSEPA (P27457) Beta-etherase (Beta-aryl ether cleaving enzyme) 31 1.4
LYM2_ARATH (O23006) LysM-domain GPI-anchored protein 2 precursor 30 4.2
YKF9_YEAST (P35728) Hypothetical 49.6 kDa protein in FBA1-TOA2 i... 29 5.5
PIM1_MOUSE (P06803) Proto-oncogene serine/threonine-protein kina... 29 7.1
PH85_YEAST (P17157) Negative regulator of the PHO system (EC 2.7... 28 9.3
PH85_CANGA (Q6FKD4) Negative regulator of the PHO system (EC 2.7... 28 9.3
MYSP_SCHJA (Q05870) Paramyosin (Antigen Sj97) 28 9.3
>YCF2_PINTH (P41653) Protein ycf2
Length = 2054
Score = 33.5 bits (75), Expect = 0.29
Identities = 29/114 (25%), Positives = 50/114 (43%), Gaps = 14/114 (12%)
Query: 75 SHFLNNKSDQL--NIGEWFLESWLVLDFEKECLGNKILEKDMKCENALVSELKLEGKRAG 132
S +NK++++ I +WF+ ECL NKI+ +D+ + L + K E
Sbjct: 956 SEMFSNKNEEIFPRIQDWFVT---------ECLKNKIVNEDIDGRSTLSNSSKEEQNIYR 1006
Query: 133 MRSEDVIGSETQLRKEVAKWINMFYSFELCKCKNIVICQNLMIILIANAECYKS 186
+ D I S+ L K W F++ CK ++ L IL+ + + S
Sbjct: 1007 ISQIDSIFSKWDLFKTYMPW---FFTSAWCKYIENMLLDTLSEILLHGSNPFVS 1057
>LIGE_PSEPA (P27457) Beta-etherase (Beta-aryl ether cleaving enzyme)
Length = 280
Score = 31.2 bits (69), Expect = 1.4
Identities = 18/77 (23%), Positives = 35/77 (45%), Gaps = 5/77 (6%)
Query: 32 DSTTYRKISMEDGAIVRARWWIDPPCKCHGGIDSLVVPVNFSNS-HFLNNKSDQLNI--- 87
++ T + +E G + W H G D +VP F+ +S+++ +
Sbjct: 4 NTITLYDLQLESGCTISPYVWRTKYALKHKGFDIDIVPGGFTGILERTGGRSERVPVIVD 63
Query: 88 -GEWFLESWLVLDFEKE 103
GEW L+SW++ ++ E
Sbjct: 64 DGEWVLDSWVIAEYLDE 80
>LYM2_ARATH (O23006) LysM-domain GPI-anchored protein 2 precursor
Length = 350
Score = 29.6 bits (65), Expect = 4.2
Identities = 17/63 (26%), Positives = 28/63 (43%), Gaps = 6/63 (9%)
Query: 3 DGAIFQNEMVDVAIKKNGLWWMGPTILYGDSTTYRKISM------EDGAIVRARWWIDPP 56
+G N ++ IKK+ + T ++G TY KIS + + ++WI P
Sbjct: 99 NGTGVSNRDIEYTIKKDDILSFVATEIFGGLVTYEKISEVNKIPDPNKIEIGQKFWIPLP 158
Query: 57 CKC 59
C C
Sbjct: 159 CSC 161
>YKF9_YEAST (P35728) Hypothetical 49.6 kDa protein in FBA1-TOA2
intergenic region
Length = 441
Score = 29.3 bits (64), Expect = 5.5
Identities = 16/51 (31%), Positives = 25/51 (48%), Gaps = 2/51 (3%)
Query: 98 LDFEKECLGNKILEKDMKCENALVSELKLEGKRAGMRSEDVIGSETQLRKE 148
+DF KE L N ++E D C N ++ L+ E + ET L+K+
Sbjct: 309 IDFSKEALENALVESDFVCPNCETRDILLDSLVPDQDKEKEV--ETFLKKQ 357
>PIM1_MOUSE (P06803) Proto-oncogene serine/threonine-protein kinase
Pim-1 (EC 2.7.1.37)
Length = 313
Score = 28.9 bits (63), Expect = 7.1
Identities = 27/113 (23%), Positives = 47/113 (40%), Gaps = 19/113 (16%)
Query: 37 RKISMEDGAIVRARWWIDPPCKCHGGIDSLVV------PVNFSNSHFLNNKSDQLNIGEW 90
+K+S + ++R W + P DS V+ PV + Q ++
Sbjct: 94 KKVSSDFSGVIRLLDWFERP-------DSFVLILERPEPVQDLFDFITERGALQEDLARG 146
Query: 91 FLESWLVLDFEKECLGNKILEKDMKCENALV----SELKLEGKRAGMRSEDVI 139
F W VL+ + C +L +D+K EN L+ E+KL +G +D +
Sbjct: 147 FF--WQVLEAVRHCHNCGVLHRDIKDENILIDLSRGEIKLIDFGSGALLKDTV 197
>PH85_YEAST (P17157) Negative regulator of the PHO system (EC
2.7.1.37) (Serine/threonine-protein kinase PHO85)
Length = 305
Score = 28.5 bits (62), Expect = 9.3
Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Query: 84 QLNIGEWFLESWLVLDFEKECLGNKILEKDMKCENALVSE 123
+LN+ ++F W +L C NKIL +D+K +N L+++
Sbjct: 106 ELNLVKYF--QWQLLQGLAFCHENKILHRDLKPQNLLINK 143
>PH85_CANGA (Q6FKD4) Negative regulator of the PHO system (EC
2.7.1.37) (Serine/threonine-protein kinase PHO85)
Length = 302
Score = 28.5 bits (62), Expect = 9.3
Identities = 13/40 (32%), Positives = 26/40 (64%), Gaps = 2/40 (5%)
Query: 84 QLNIGEWFLESWLVLDFEKECLGNKILEKDMKCENALVSE 123
++N+ ++F W +L+ C NKIL +D+K +N L+++
Sbjct: 105 EMNLVKYF--QWQLLEGLAFCHENKILHRDLKPQNLLITK 142
>MYSP_SCHJA (Q05870) Paramyosin (Antigen Sj97)
Length = 866
Score = 28.5 bits (62), Expect = 9.3
Identities = 31/111 (27%), Positives = 52/111 (45%), Gaps = 10/111 (9%)
Query: 39 ISMEDGAIVR-ARWWIDPPCKCHGGIDSLVVPVNFSNSHFLNNKSDQLNIGEWFLESWLV 97
+S+E+G ++R A+ + +D L + VN L ++++QL L+S LV
Sbjct: 351 LSLENGELIRRAKSAESLASELQRRVDELTIEVNT-----LTSQNNQLESENMRLKS-LV 404
Query: 98 LDFEKECLGNKILEKDMKCENALVSELKLEGKRAGMRSEDVIGSETQLRKE 148
D + N LE++ + N V ELK + A R D+ +QL E
Sbjct: 405 NDLTDK---NNALERENRQMNDQVKELKSSLRDANRRLTDLEALRSQLEAE 452
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,549,969
Number of Sequences: 164201
Number of extensions: 891383
Number of successful extensions: 1684
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1683
Number of HSP's gapped (non-prelim): 8
length of query: 186
length of database: 59,974,054
effective HSP length: 104
effective length of query: 82
effective length of database: 42,897,150
effective search space: 3517566300
effective search space used: 3517566300
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0103.3