BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0100a.5
(201 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Y20L_ARATH (O80813) Ycf20-like protein 186 2e-47
YC20_MESVI (Q9MUL5) Hypothetical 13.0 kDa protein ycf20 (RF20) 96 6e-20
YC20_PORPU (P51214) Hypothetical 11.9 kDa protein ycf20 (ORF108) 72 7e-13
Y20L_SYNY3 (P72983) Ycf20-like protein 67 2e-11
YC20_CYACA (Q9MVP1) Hypothetical 11.5 kDa protein ycf20 59 6e-09
YC20_GALSU (P48409) Hypothetical 9.5 kDa protein ycf20 53 5e-07
YC20_AGLNE (P34813) Hypothetical 11.4 kDa protein ycf20 52 1e-06
YC20_GUITH (O78445) Hypothetical 7.2 kDa protein ycf20 51 2e-06
KUP_PSEPK (Q88NK7) Probable potassium transport system protein kup 33 0.34
Y444_LISIN (Q92EL4) Putative sugar uptake protein lin0444 33 0.44
Y424_LISMO (Q8Y9U6) Putative sugar uptake protein lmo0424 33 0.44
N116_YEAST (Q02630) Nucleoporin NUP116/NSP116 (Nuclear pore prot... 31 2.2
SECY_MAIZE (O63066) Preprotein translocase secY subunit, chlorop... 30 3.7
HMDH_PICJA (O74164) 3-hydroxy-3-methylglutaryl-coenzyme A reduct... 30 3.7
Y680_CHLPN (Q9Z7M4) Putative phosphate permease CPn0680/CP0067/C... 29 6.4
PHT1_PSEPU (Q05181) Phthalate transporter 29 6.4
HORN_HUMAN (Q86YZ3) Hornerin 29 6.4
ROC2_NICSY (Q08937) 29 kDa ribonucleoprotein B, chloroplast prec... 29 8.3
ROC2_NICPL (P49314) 31 kDa ribonucleoprotein, chloroplast precur... 29 8.3
AZR1_SCHPO (Q09189) 5-azacytidine resistance protein azr1 29 8.3
>Y20L_ARATH (O80813) Ycf20-like protein
Length = 121
Score = 186 bits (473), Expect = 2e-47
Identities = 91/121 (75%), Positives = 107/121 (88%), Gaps = 1/121 (0%)
Query: 82 LNDTSSSFGGTRLGRILSAGGRQLLDKLNSARKNVPMKIFLLLLGFYTANALATILGQTG 141
+N + G TRLGRI AGG+QLL K+NSARKN PMKIFLLLLGFYTANALATILGQTG
Sbjct: 1 MNGDRTGLGSTRLGRIAIAGGKQLLGKINSARKNFPMKIFLLLLGFYTANALATILGQTG 60
Query: 142 DWDVLVAGVVVAAIEGIGMLLYRKP-PTVRTGRLQSFLLMLNYWKAGICLGLFVDAFKLG 200
DWDVLVAG+VVAAIEGIGML+Y+KP ++ +G+LQSF++ +N+WKAG+CLGLFVDAFKLG
Sbjct: 61 DWDVLVAGIVVAAIEGIGMLMYKKPSSSMFSGKLQSFVVFMNFWKAGVCLGLFVDAFKLG 120
Query: 201 S 201
S
Sbjct: 121 S 121
>YC20_MESVI (Q9MUL5) Hypothetical 13.0 kDa protein ycf20 (RF20)
Length = 116
Score = 95.9 bits (237), Expect = 6e-20
Identities = 56/102 (54%), Positives = 69/102 (66%), Gaps = 4/102 (3%)
Query: 104 QLLD-KLNSARKNVPMKIFLLLLGFYTANALATILGQTGDWDVLVAGVVVAAIEGIGMLL 162
QLLD K+ + N P+KI LLLLGF+ A LAT+ GQTGDWDVLVAG++VA IE +G +
Sbjct: 15 QLLDSKIQNYSINFPIKILLLLLGFFIATVLATVFGQTGDWDVLVAGILVAMIEILGNKM 74
Query: 163 YRKPPTVRTGRLQ-SFLLM--LNYWKAGICLGLFVDAFKLGS 201
Y K + SFL + +NY K G+ GLFVDAFKLGS
Sbjct: 75 YSKKYISKKQVFDISFLSLIGINYIKIGLIFGLFVDAFKLGS 116
>YC20_PORPU (P51214) Hypothetical 11.9 kDa protein ycf20 (ORF108)
Length = 108
Score = 72.4 bits (176), Expect = 7e-13
Identities = 45/112 (40%), Positives = 64/112 (56%), Gaps = 9/112 (8%)
Query: 92 TRLGRILSAGGRQLLDKLNSARKNVPMKIFLLLLGFYTANALATILGQTGDWDVLVAGVV 151
T+L S + L +L + + + LLLGF+ + L+TI GQTGDW ++ A ++
Sbjct: 4 TKLSIFFSYFVQNLSSRLYYSLNELTAGLISLLLGFFISTGLSTIPGQTGDWGIIAASLI 63
Query: 152 VAAIEGIGMLLY--RKPPTVRTGRLQSFLLMLNYWKAGICLGLFVDAFKLGS 201
VAAIE + ++Y +K VR + +LN K GI GLFVDAFKLGS
Sbjct: 64 VAAIELVSKIVYSNKKKYGVR-------INLLNNLKIGITYGLFVDAFKLGS 108
>Y20L_SYNY3 (P72983) Ycf20-like protein
Length = 109
Score = 67.4 bits (163), Expect = 2e-11
Identities = 38/110 (34%), Positives = 59/110 (53%), Gaps = 4/110 (3%)
Query: 92 TRLGRILSAGGRQLLDKLNSARKNVPMKIFLLLLGFYTANALATILGQTGDWDVLVAGVV 151
TRL I+ G+QL + + + + + L GF+ A+AT GQ WDV+ A +
Sbjct: 4 TRLNTIVEVRGQQLSQFFRNPWRRISLSLLSFLFGFFVGTAVATTAGQNSQWDVVCAAFI 63
Query: 152 VAAIEGIGMLLYRKPPTVRTGRLQSFLLMLNYWKAGICLGLFVDAFKLGS 201
+ E + YR+ V+ G LQ+ +LN +K G+ LF++AFKLGS
Sbjct: 64 LLFCELVNRWFYRR--GVKMGDLQA--EVLNIFKMGVSYSLFLEAFKLGS 109
>YC20_CYACA (Q9MVP1) Hypothetical 11.5 kDa protein ycf20
Length = 101
Score = 59.3 bits (142), Expect = 6e-09
Identities = 30/81 (37%), Positives = 47/81 (57%), Gaps = 1/81 (1%)
Query: 121 FLLLLGFYTANALATILGQTGDWDVLVAGVVVAAIEGIGMLLYRKPPTVRTGRLQSFLLM 180
F+ L GF+ ++A +TIL QT +W +L A ++++ +E L ++ R F
Sbjct: 22 FIFLFGFFLSSATSTILIQTNEWSILTAAILISIVELFNYLKHKFQFNDRKSGYNCF-FF 80
Query: 181 LNYWKAGICLGLFVDAFKLGS 201
+N K G+ GLF+DAFKLGS
Sbjct: 81 INLAKLGLLYGLFIDAFKLGS 101
>YC20_GALSU (P48409) Hypothetical 9.5 kDa protein ycf20
Length = 83
Score = 52.8 bits (125), Expect = 5e-07
Identities = 28/78 (35%), Positives = 44/78 (55%), Gaps = 3/78 (3%)
Query: 124 LLGFYTANALATILGQTGDWDVLVAGVVVAAIEGIGMLLYRKPPTVRTGRLQSFLLMLNY 183
LLG +++N L TI QTGDW + + ++A E I + Y + ++ + N
Sbjct: 9 LLGIFSSNLLCTIYTQTGDWSLYLTSCIIALYEIISYISYNQ---FIKKTHKNIINCFNG 65
Query: 184 WKAGICLGLFVDAFKLGS 201
+K G+ GL++DAFKLGS
Sbjct: 66 FKIGLIYGLYLDAFKLGS 83
>YC20_AGLNE (P34813) Hypothetical 11.4 kDa protein ycf20
Length = 100
Score = 51.6 bits (122), Expect = 1e-06
Identities = 28/87 (32%), Positives = 48/87 (54%), Gaps = 4/87 (4%)
Query: 115 NVPMKIFLLLLGFYTANALATILGQTGDWDVLVAGVVVAAIEGIGMLLYRKPPTVRTGRL 174
N+ +++ L LG++ + +I +TGDW ++ A ++V E + L+Y + +
Sbjct: 18 NLSLQLISLFLGYFFCTIICSIPKETGDWGLITALLIVGINETMSALIY----SYKKYEN 73
Query: 175 QSFLLMLNYWKAGICLGLFVDAFKLGS 201
+ +N K GI GLFVD+FKLGS
Sbjct: 74 NIIVKTINGIKIGIIYGLFVDSFKLGS 100
>YC20_GUITH (O78445) Hypothetical 7.2 kDa protein ycf20
Length = 64
Score = 50.8 bits (120), Expect = 2e-06
Identities = 22/50 (44%), Positives = 32/50 (64%)
Query: 120 IFLLLLGFYTANALATILGQTGDWDVLVAGVVVAAIEGIGMLLYRKPPTV 169
+ +L GF+ + L+TIL QTGDW +L A ++VA IE I Y+K P +
Sbjct: 8 LLTILFGFFLSTLLSTILSQTGDWSILAASILVATIELINKNKYKKKPLI 57
>KUP_PSEPK (Q88NK7) Probable potassium transport system protein kup
Length = 636
Score = 33.5 bits (75), Expect = 0.34
Identities = 20/61 (32%), Positives = 35/61 (56%), Gaps = 3/61 (4%)
Query: 120 IFLLLLGFYTANALATILGQTGDWDVLVAGVVVAAIEGIGMLLYRKPPTVRTGRLQSFLL 179
+ LL++GF ++ ALA G +L+ V+V+A+ +LL++ PP + L FLL
Sbjct: 364 VVLLVIGFESSGALAAAYGVAVTGTMLITTVLVSAVM---LLLWKWPPLLAVPILVGFLL 420
Query: 180 M 180
+
Sbjct: 421 V 421
>Y444_LISIN (Q92EL4) Putative sugar uptake protein lin0444
Length = 285
Score = 33.1 bits (74), Expect = 0.44
Identities = 29/94 (30%), Positives = 42/94 (43%), Gaps = 9/94 (9%)
Query: 94 LGRILSAGGRQLLDKLNSARKNVPMKIFLLL------LGFYTANALATILGQTGDWDVLV 147
+G + A G L K +RKNV + L LG + A A +LG + +
Sbjct: 185 IGMLTCAIGINLAKKTAISRKNVTFNLMTGLSWSIANLGMFLATA---VLGVATSFSISQ 241
Query: 148 AGVVVAAIEGIGMLLYRKPPTVRTGRLQSFLLML 181
A V+VA I GI + +K P T L LL++
Sbjct: 242 ACVIVATIGGILIFKQKKSPLEWTFILSGILLIM 275
>Y424_LISMO (Q8Y9U6) Putative sugar uptake protein lmo0424
Length = 285
Score = 33.1 bits (74), Expect = 0.44
Identities = 29/94 (30%), Positives = 42/94 (43%), Gaps = 9/94 (9%)
Query: 94 LGRILSAGGRQLLDKLNSARKNVPMKIFLLL------LGFYTANALATILGQTGDWDVLV 147
+G + A G L K +RKNV + L LG + A A +LG + +
Sbjct: 185 IGMLTCAIGINLAKKTAISRKNVTFNLMTGLSWSIANLGMFLATA---VLGVATSFSISQ 241
Query: 148 AGVVVAAIEGIGMLLYRKPPTVRTGRLQSFLLML 181
A V+VA I GI + +K P T L LL++
Sbjct: 242 ACVIVATIGGILIFKQKKSPLEWTFILSGILLIM 275
>N116_YEAST (Q02630) Nucleoporin NUP116/NSP116 (Nuclear pore protein
NUP116/NSP116)
Length = 1113
Score = 30.8 bits (68), Expect = 2.2
Identities = 20/66 (30%), Positives = 33/66 (49%), Gaps = 4/66 (6%)
Query: 40 TSNAGGKSSHFPQSGFLGRKAVWKIAFALNTGGVPGNGEQQSLNDTSSS---FGGTRLGR 96
++NA ++ +G G K V A + + GG+ GN SLN+++ S FG +
Sbjct: 634 SNNASNTAATTNSTGLFGNKPVGAGA-STSAGGLFGNNNNSSLNNSNGSTGLFGSNNTSQ 692
Query: 97 ILSAGG 102
+AGG
Sbjct: 693 STNAGG 698
>SECY_MAIZE (O63066) Preprotein translocase secY subunit,
chloroplast precursor (CpSecY)
Length = 553
Score = 30.0 bits (66), Expect = 3.7
Identities = 38/123 (30%), Positives = 54/123 (43%), Gaps = 21/123 (17%)
Query: 56 LGRKAVWKIAFALNTGGV-----PGNGEQQSLNDTSSSFGGTRLGR--ILSAGGRQLLDK 108
LG A+ ++ + GGV GN +Q SL T SF G +GR I S G ++
Sbjct: 148 LGFLALSRLGVYIPLGGVNREAFAGNLDQNSLLGTLDSFSGGGIGRLGICSLG---IVPF 204
Query: 109 LNSARKNVPMKIFLLLLGFY-TANALATILGQTGDWDVL----VAGVVVAAIEGIGMLLY 163
+N+ +F LL Y L G+ G VL A V A ++ IG +LY
Sbjct: 205 INA------QIVFQLLAQLYPKLQDLQRKEGEAGRKKVLQYTRYASVGFAIVQAIGQVLY 258
Query: 164 RKP 166
+P
Sbjct: 259 LRP 261
>HMDH_PICJA (O74164) 3-hydroxy-3-methylglutaryl-coenzyme A reductase
(EC 1.1.1.34) (HMG-CoA reductase)
Length = 934
Score = 30.0 bits (66), Expect = 3.7
Identities = 22/70 (31%), Positives = 31/70 (43%), Gaps = 12/70 (17%)
Query: 14 SSKKPYTAFSASRVHGCCCEPILSGLTSNAG--------GKSSHFPQS---GFLGRKAVW 62
S+K PY + RV G CCE ++ + G GK H P + G L A+
Sbjct: 568 SNKLPYLHYDYDRVFGACCENVIGYMPLPVGVAGPLIIDGKPYHIPMATTEGCLVASAM- 626
Query: 63 KIAFALNTGG 72
+ A+N GG
Sbjct: 627 RGCKAINLGG 636
>Y680_CHLPN (Q9Z7M4) Putative phosphate permease
CPn0680/CP0067/CPj0680/CpB0707
Length = 426
Score = 29.3 bits (64), Expect = 6.4
Identities = 22/55 (40%), Positives = 29/55 (52%), Gaps = 8/55 (14%)
Query: 116 VPMKIFLLLLGFYT-----ANALATILGQTGDWDVLV---AGVVVAAIEGIGMLL 162
+P+ IF+LL GFYT AN +A +G + VL A V+ A E G LL
Sbjct: 2 LPLIIFVLLCGFYTSWNIGANDVANAVGPSVGSGVLTLRQAVVIAAIFEFFGALL 56
>PHT1_PSEPU (Q05181) Phthalate transporter
Length = 451
Score = 29.3 bits (64), Expect = 6.4
Identities = 25/103 (24%), Positives = 44/103 (42%), Gaps = 12/103 (11%)
Query: 100 AGGRQLLDKLNSARKNVP-MKIFLLLLGFYTANALATILG--------QTGDWDVLVAGV 150
AG + + DK ++ + ++++L G+ T TIL TG DVL G+
Sbjct: 240 AGKKPVTDKRHAVLAALKDPRVYVLAAGWATVPLCGTILNYWTPTIIRNTGIQDVLHVGL 299
Query: 151 VVAA---IEGIGMLLYRKPPTVRTGRLQSFLLMLNYWKAGICL 190
+ + I M+L + +R R + F + + G CL
Sbjct: 300 LSTVPYIVGAIAMILIARSSDIRLERRKHFFFSIAFGALGACL 342
>HORN_HUMAN (Q86YZ3) Hornerin
Length = 2850
Score = 29.3 bits (64), Expect = 6.4
Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 3/83 (3%)
Query: 22 FSASRVHGCCC-EPILSGLTSNAGGKSSHFPQSGFLGRKAVWKIAFALNTGGVPGNGEQQ 80
FS+ HG + SG ++ G+SS + Q A + +G PG+G++
Sbjct: 838 FSSQGRHGSTSGQSSSSGQHDSSSGQSSSYGQHESASHHASGRGRHGSGSGQSPGHGQRG 897
Query: 81 SLNDTSSSFG--GTRLGRILSAG 101
S + S S+G G+ GR S+G
Sbjct: 898 SGSGQSPSYGRHGSGSGRSSSSG 920
>ROC2_NICSY (Q08937) 29 kDa ribonucleoprotein B, chloroplast
precursor (CP29B)
Length = 291
Score = 28.9 bits (63), Expect = 8.3
Identities = 16/49 (32%), Positives = 24/49 (48%)
Query: 71 GGVPGNGEQQSLNDTSSSFGGTRLGRILSAGGRQLLDKLNSARKNVPMK 119
GG GN D +SSFGG R GR + + R + L+ ++ +K
Sbjct: 176 GGRGGNSSYGGGRDGNSSFGGARGGRSVDSSNRVYVGNLSWGVDDLALK 224
>ROC2_NICPL (P49314) 31 kDa ribonucleoprotein, chloroplast precursor
(CP-RBP31)
Length = 292
Score = 28.9 bits (63), Expect = 8.3
Identities = 16/49 (32%), Positives = 24/49 (48%)
Query: 71 GGVPGNGEQQSLNDTSSSFGGTRLGRILSAGGRQLLDKLNSARKNVPMK 119
GG GN D +SSFGG R GR + + R + L+ ++ +K
Sbjct: 177 GGRGGNSSYGGGRDGNSSFGGARGGRSVDSSNRVYVGNLSWGVDDLALK 225
>AZR1_SCHPO (Q09189) 5-azacytidine resistance protein azr1
Length = 288
Score = 28.9 bits (63), Expect = 8.3
Identities = 24/84 (28%), Positives = 39/84 (45%), Gaps = 6/84 (7%)
Query: 75 GNGEQQSLNDTSSSFGGTRLGRILSAGGRQLLDKLNSARKNVPMKIFLLLLGFYTANALA 134
GNG+ SLN S F R G I A Q+L + N+P ++ + + +A +
Sbjct: 138 GNGKLHSLNLGDSGFLILRNGAIHYASPAQVL------QFNMPYQLAIYPRNYRSAENIG 191
Query: 135 TILGQTGDWDVLVAGVVVAAIEGI 158
+GQ D+ +V+ A +GI
Sbjct: 192 PKMGQATVHDLKDNDLVILATDGI 215
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.138 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,748,978
Number of Sequences: 164201
Number of extensions: 969116
Number of successful extensions: 2240
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2216
Number of HSP's gapped (non-prelim): 26
length of query: 201
length of database: 59,974,054
effective HSP length: 105
effective length of query: 96
effective length of database: 42,732,949
effective search space: 4102363104
effective search space used: 4102363104
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0100a.5