BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0100a.14
(90 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CFB5_HUMAN (Q9P1F3) Protein C6orf115 (PRO2013) 108 2e-24
ISPE_BACCR (Q81JA2) 4-diphosphocytidyl-2-C-methyl-D-erythritol k... 32 0.37
ISPE_BACC1 (Q73FG3) 4-diphosphocytidyl-2-C-methyl-D-erythritol k... 31 0.48
ISPE_BACAN (Q81VZ6) 4-diphosphocytidyl-2-C-methyl-D-erythritol k... 31 0.48
HPS5_HUMAN (Q9UPZ3) Hermansky-Pudlak syndrome 5 protein (Alpha-i... 29 2.4
SWI4_YEAST (P25302) Regulatory protein SWI4 (Cell-cycle box fact... 28 3.1
PYC_MOUSE (Q05920) Pyruvate carboxylase, mitochondrial precursor... 28 4.1
ISPG_THETH (Q84GJ3) 4-hydroxy-3-methylbut-2-en-1-yl diphosphate ... 28 4.1
ISPG_THET2 (Q72H18) 4-hydroxy-3-methylbut-2-en-1-yl diphosphate ... 28 4.1
UBP5_MOUSE (P56399) Ubiquitin carboxyl-terminal hydrolase 5 (EC ... 28 5.3
CLPB_MYCPN (P75247) Chaperone clpB 28 5.3
POLG_CXA21 (P22055) Genome polyprotein [Contains: Coat protein V... 27 7.0
TACY_BACCE (Q45105) Hemolysin precursor (Fragment) 27 9.1
RR4_CHLRE (P48270) Chloroplast 30S ribosomal protein S4 27 9.1
POLG_POL3L (P03302) Genome polyprotein [Contains: Coat protein V... 27 9.1
ISPG_RALSO (P58669) 4-hydroxy-3-methylbut-2-en-1-yl diphosphate ... 27 9.1
ISPG_DEIRA (Q9RXC9) 4-hydroxy-3-methylbut-2-en-1-yl diphosphate ... 27 9.1
HPS5_MOUSE (P59438) Hermansky-Pudlak syndrome 5 protein homolog ... 27 9.1
ECT1_SCHPO (Q9UTI6) Probable ethanolamine-phosphate cytidylyltra... 27 9.1
>CFB5_HUMAN (Q9P1F3) Protein C6orf115 (PRO2013)
Length = 81
Score = 108 bits (271), Expect = 2e-24
Identities = 54/78 (69%), Positives = 61/78 (77%)
Query: 1 MNVEEEVQRLKEEITRLGSVQTDGSYKVTFGTLFHDDRCANIFEALVGTLRAAKKRKIVA 60
MNV+ EV L EEI RLGS DG V FG LF DD+CAN+FEALVGTL+AAK+RKIV
Sbjct: 1 MNVDHEVNLLVEEIHRLGSKNADGKLSVKFGVLFRDDKCANLFEALVGTLKAAKRRKIVT 60
Query: 61 YDGELLLQGVHDNVEITL 78
Y GELLLQGVHD+V+I L
Sbjct: 61 YPGELLLQGVHDDVDIIL 78
>ISPE_BACCR (Q81JA2) 4-diphosphocytidyl-2-C-methyl-D-erythritol
kinase (EC 2.7.1.148) (CMK)
(4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol
kinase)
Length = 289
Score = 31.6 bits (70), Expect = 0.37
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 2/45 (4%)
Query: 6 EVQRLKEEITRLGS--VQTDGSYKVTFGTLFHDDRCANIFEALVG 48
EV R+K ++ R G+ V GS FG + HD R I+ L G
Sbjct: 227 EVARIKSQMKRFGADAVLMSGSGPTVFGLVHHDSRMHRIYNGLKG 271
>ISPE_BACC1 (Q73FG3) 4-diphosphocytidyl-2-C-methyl-D-erythritol
kinase (EC 2.7.1.148) (CMK)
(4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol
kinase)
Length = 289
Score = 31.2 bits (69), Expect = 0.48
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 2/45 (4%)
Query: 6 EVQRLKEEITRLGS--VQTDGSYKVTFGTLFHDDRCANIFEALVG 48
EV R+K ++ R G+ V GS FG + HD R I+ L G
Sbjct: 227 EVARIKAQMKRFGADAVLMSGSGPTVFGLVHHDSRMHRIYNGLKG 271
>ISPE_BACAN (Q81VZ6) 4-diphosphocytidyl-2-C-methyl-D-erythritol
kinase (EC 2.7.1.148) (CMK)
(4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol
kinase)
Length = 289
Score = 31.2 bits (69), Expect = 0.48
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 2/45 (4%)
Query: 6 EVQRLKEEITRLGS--VQTDGSYKVTFGTLFHDDRCANIFEALVG 48
EV R+K ++ R G+ V GS FG + HD R I+ L G
Sbjct: 227 EVARIKAQMKRFGADAVLMSGSGPTVFGLVHHDSRMHRIYNGLKG 271
>HPS5_HUMAN (Q9UPZ3) Hermansky-Pudlak syndrome 5 protein
(Alpha-integrin-binding protein 63) (Ruby-eye protein 2
homolog) (Ru2)
Length = 1129
Score = 28.9 bits (63), Expect = 2.4
Identities = 15/56 (26%), Positives = 28/56 (49%), Gaps = 4/56 (7%)
Query: 11 KEEITRLGSVQTDGSYKVTFGTLFHDDRCANIFEALVGTLRAAKKRKIVAYDGELL 66
+E+ ++G+ + DG Y G F RC+ + L+ R + V +DGE++
Sbjct: 204 REKFWKIGNKERDGEY----GACFFPGRCSGGQQPLIYCARPGSRMWEVNFDGEVI 255
>SWI4_YEAST (P25302) Regulatory protein SWI4 (Cell-cycle box factor,
chain SWI4) (ART1 protein)
Length = 1093
Score = 28.5 bits (62), Expect = 3.1
Identities = 14/40 (35%), Positives = 23/40 (57%)
Query: 16 RLGSVQTDGSYKVTFGTLFHDDRCANIFEALVGTLRAAKK 55
+L + TD YK T T+ +R +++ +AL+ TL A K
Sbjct: 470 KLENKMTDQEYKQTILTILSSERSSDVDQALLATLYPAPK 509
>PYC_MOUSE (Q05920) Pyruvate carboxylase, mitochondrial precursor
(EC 6.4.1.1) (Pyruvic carboxylase) (PCB)
Length = 1178
Score = 28.1 bits (61), Expect = 4.1
Identities = 25/89 (28%), Positives = 43/89 (48%), Gaps = 9/89 (10%)
Query: 4 EEEVQRLKEEITRLGSVQTDGSYKV-TFGTLFHDDRCANIFEALVGTLRAAKKR--KIVA 60
E V+ +K I L +V + + T T F D+ +F+ LR A+ R K++
Sbjct: 449 EFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDEN-PELFQ-----LRPAQNRAQKLLH 502
Query: 61 YDGELLLQGVHDNVEITLNPSPAAPAAAV 89
Y G +++ G + + ++PSP PA V
Sbjct: 503 YLGHVMVNGPTTPIPVNVSPSPVDPAVPV 531
>ISPG_THETH (Q84GJ3) 4-hydroxy-3-methylbut-2-en-1-yl diphosphate
synthase (EC 1.17.4.3) (1-hydroxy-2-methyl-2-(E)-butenyl
4-diphosphate synthase)
Length = 406
Score = 28.1 bits (61), Expect = 4.1
Identities = 10/21 (47%), Positives = 15/21 (70%)
Query: 65 LLLQGVHDNVEITLNPSPAAP 85
LLL+G+ D + ++L PSP P
Sbjct: 250 LLLEGIGDTIRVSLTPSPKEP 270
>ISPG_THET2 (Q72H18) 4-hydroxy-3-methylbut-2-en-1-yl diphosphate
synthase (EC 1.17.4.3) (1-hydroxy-2-methyl-2-(E)-butenyl
4-diphosphate synthase)
Length = 406
Score = 28.1 bits (61), Expect = 4.1
Identities = 10/21 (47%), Positives = 15/21 (70%)
Query: 65 LLLQGVHDNVEITLNPSPAAP 85
LLL+G+ D + ++L PSP P
Sbjct: 250 LLLEGIGDTIRVSLTPSPKEP 270
>UBP5_MOUSE (P56399) Ubiquitin carboxyl-terminal hydrolase 5 (EC
3.1.2.15) (Ubiquitin thiolesterase 5)
(Ubiquitin-specific processing protease 5)
(Deubiquitinating enzyme 5) (Isopeptidase T)
Length = 858
Score = 27.7 bits (60), Expect = 5.3
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Query: 37 DRCANIFEALVGTLRAAKKRKIVAYDGELLLQGVHD-NVEITLNPSPAAP 85
DR + EAL+ A++K+++ A+DGE+ H N++ NP+ P
Sbjct: 145 DRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPP 194
>CLPB_MYCPN (P75247) Chaperone clpB
Length = 715
Score = 27.7 bits (60), Expect = 5.3
Identities = 14/36 (38%), Positives = 22/36 (60%), Gaps = 4/36 (11%)
Query: 5 EEVQRLKEEI----TRLGSVQTDGSYKVTFGTLFHD 36
E + +LK+EI TRL QT+G+Y+ L++D
Sbjct: 330 ESINKLKKEIEDLQTRLELYQTEGNYEAASKILYYD 365
>POLG_CXA21 (P22055) Genome polyprotein [Contains: Coat protein VP4
(P1A); Coat protein VP2 (P1B); Coat protein VP3 (P1C);
Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29) (Core
protein P2A); Core protein P2B; Core protein P2C; Core
protein P3A; Genome
Length = 2206
Score = 27.3 bits (59), Expect = 7.0
Identities = 18/41 (43%), Positives = 21/41 (50%), Gaps = 5/41 (12%)
Query: 52 AAKKRKIVAYD---GELLLQGVHDNVEITLNPSPAAPAAAV 89
A KR IV GE + GVHDNV I P+ AAP +
Sbjct: 1571 AMAKRNIVTATTTKGEFTMLGVHDNVAIL--PTHAAPGETI 1609
>TACY_BACCE (Q45105) Hemolysin precursor (Fragment)
Length = 485
Score = 26.9 bits (58), Expect = 9.1
Identities = 22/81 (27%), Positives = 37/81 (45%), Gaps = 9/81 (11%)
Query: 5 EEVQRLKEEITRLGSVQTDGSYKVTFGTLFHDDRCANIFEALVGTLRAAKKRKIVAYDGE 64
++VQ + + + SV+T G YK F + F A+V A + K+V D
Sbjct: 295 KDVQAAFKALLKNNSVETSGQYKDIFE--------ESTFTAVVLGGDAKEHNKVVTKDFN 346
Query: 65 LLLQGVHDNVEITLNPSPAAP 85
+ + DN E++ +PA P
Sbjct: 347 EIRNIIKDNAELSFK-NPAYP 366
>RR4_CHLRE (P48270) Chloroplast 30S ribosomal protein S4
Length = 257
Score = 26.9 bits (58), Expect = 9.1
Identities = 19/45 (42%), Positives = 25/45 (55%), Gaps = 5/45 (11%)
Query: 46 LVGTLRAAKKRKIVAYDGELLLQGVH---DNVEITLNPSPAAPAA 87
LV +R AKK K G++LLQ + DN+ LN +P PAA
Sbjct: 86 LVNYVRKAKKIK--ESTGQVLLQFLEMRLDNIVFRLNMAPTIPAA 128
>POLG_POL3L (P03302) Genome polyprotein [Contains: Coat protein VP4
(P1A); Coat protein VP2 (P1B); Coat protein VP3 (P1C);
Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29) (Core
protein P2A); Core protein P2B; Core protein P2C; Core
protein P3A; Genome
Length = 2206
Score = 26.9 bits (58), Expect = 9.1
Identities = 22/59 (37%), Positives = 28/59 (47%), Gaps = 18/59 (30%)
Query: 47 VGTLRAAK-------------KRKIVAYD---GELLLQGVHDNVEITLNPSPAAPAAAV 89
V T+RAAK KR IV GE + GVHDNV I P+ A+P ++
Sbjct: 1553 VPTIRAAKVQGPGFDYAVAMAKRNIVTATTSKGEFTMLGVHDNVAIL--PTHASPGESI 1609
>ISPG_RALSO (P58669) 4-hydroxy-3-methylbut-2-en-1-yl diphosphate
synthase (EC 1.17.4.3) (1-hydroxy-2-methyl-2-(E)-butenyl
4-diphosphate synthase)
Length = 431
Score = 26.9 bits (58), Expect = 9.1
Identities = 10/21 (47%), Positives = 14/21 (66%)
Query: 65 LLLQGVHDNVEITLNPSPAAP 85
LL +G+ D + I+L P P AP
Sbjct: 268 LLQEGIGDTIRISLTPEPGAP 288
>ISPG_DEIRA (Q9RXC9) 4-hydroxy-3-methylbut-2-en-1-yl diphosphate
synthase (EC 1.17.4.3) (1-hydroxy-2-methyl-2-(E)-butenyl
4-diphosphate synthase)
Length = 429
Score = 26.9 bits (58), Expect = 9.1
Identities = 9/21 (42%), Positives = 14/21 (65%)
Query: 65 LLLQGVHDNVEITLNPSPAAP 85
LL +G+ D + ++L P P AP
Sbjct: 270 LLTEGIGDTIRVSLTPEPGAP 290
>HPS5_MOUSE (P59438) Hermansky-Pudlak syndrome 5 protein homolog
(Ruby-eye protein 2) (Ru2)
Length = 1126
Score = 26.9 bits (58), Expect = 9.1
Identities = 15/56 (26%), Positives = 27/56 (47%), Gaps = 4/56 (7%)
Query: 11 KEEITRLGSVQTDGSYKVTFGTLFHDDRCANIFEALVGTLRAAKKRKIVAYDGELL 66
+E+ ++G+ + G Y G F RCA + ++ R + V +DGE+L
Sbjct: 204 REKFWKIGNKERHGEY----GACFFPGRCAGGQQPVIYCARPGSRMWEVNFDGEVL 255
>ECT1_SCHPO (Q9UTI6) Probable ethanolamine-phosphate
cytidylyltransferase (EC 2.7.7.14)
(Phosphorylethanolamine transferase)
(CTP:phosphoethanolamine cytidylyltransferase)
Length = 365
Score = 26.9 bits (58), Expect = 9.1
Identities = 11/20 (55%), Positives = 14/20 (70%)
Query: 63 GELLLQGVHDNVEITLNPSP 82
GE L+ G+H + EITLN P
Sbjct: 35 GETLVIGIHSDEEITLNKGP 54
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.134 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,073,593
Number of Sequences: 164201
Number of extensions: 335941
Number of successful extensions: 1042
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1035
Number of HSP's gapped (non-prelim): 19
length of query: 90
length of database: 59,974,054
effective HSP length: 66
effective length of query: 24
effective length of database: 49,136,788
effective search space: 1179282912
effective search space used: 1179282912
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0100a.14