BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0083.16
(294 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HOW_DROME (O01367) Held out wings protein (KH-domain protein KH9... 130 5e-30
GLD1_CAEEL (Q17339) Female germline-specific tumor suppressor gl... 123 6e-28
YKCA_CAEEL (P42083) Hypothetical protein B0280.11 in chromosome III 95 2e-19
SF01_HUMAN (Q15637) Splicing factor 1 (Zinc finger protein 162) ... 91 4e-18
SF01_MOUSE (Q64213) Splicing factor 1 (Zinc finger protein 162) ... 89 1e-17
GRP3_ARTSA (P13230) Glycine-rich protein GRP33 75 3e-13
YAJE_SCHPO (Q09911) Hypothetical protein C30D11.14c in chromosome I 39 0.012
YCF9_YEAST (P25586) Hypothetical 37.2 kDa protein in CHA1-PRD1 i... 33 0.64
HRB2_MOUSE (Q8BGA5) HIV-1 Rev binding protein 2 homolog 33 0.84
FENR_SYNP2 (P31973) Ferredoxin--NADP reductase (EC 1.18.1.2) (FNR) 33 0.84
ECR1_METTH (O26780) Probable exosome complex RNA-binding protein 1 33 0.84
YG5T_YEAST (P53332) Hypothetical 34.3 kDa protein in TAF145-YOR1... 33 1.1
HRB2_HUMAN (Q13601) HIV-1 Rev binding protein 2 (Rev interacting... 33 1.1
TBP7_CAEEL (P54816) TAT-binding homolog 7 32 1.4
RA50_AQUAE (O67124) Probable DNA double-strand break repair rad5... 32 1.4
CIRH_HUMAN (Q969X6) Cirhin 32 1.9
CIRH_MOUSE (Q8R2N2) Cirhin (Testis expressed gene 292 protein) (... 32 2.4
RECN_HELPY (O25943) DNA repair protein recN (Recombination prote... 31 3.2
S160_YEAST (P06105) SCP160 protein (Protein HX) 30 5.4
RECN_HELPJ (Q9ZJ80) DNA repair protein recN (Recombination prote... 30 5.4
>HOW_DROME (O01367) Held out wings protein (KH-domain protein KH93F)
(Putative RNA-binding protein) (Muscle-specific protein)
(Wings held out protein) (Struthio protein)
(Quaking-related 93F)
Length = 405
Score = 130 bits (326), Expect = 5e-30
Identities = 82/228 (35%), Positives = 118/228 (50%), Gaps = 44/228 (19%)
Query: 42 YLTELLGERHILGPFMAVLPHCYRLINQEILRVTTLLGNASVLGQSRLEHASPLATGGIF 101
YL +LL +R L F V H RL+++EI RV L
Sbjct: 78 YLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASL----------------------- 114
Query: 102 SNGGADVNGWASRFQSEMPSLLQSSATPSWLSPQGSSSGLVVKKTMRVDIPVEVYPNFNF 161
+NG + P L P+GS VV +V +PV +P+FNF
Sbjct: 115 ----FQING-----VKKEPLTLPE--------PEGS----VVTMNEKVYVPVREHPDFNF 153
Query: 162 VGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPAKEEMMRGKPGYEHLNEPLHVLVEAE 221
VGR+LGPRG + K++E T C++++RG+GS++D KE+ RGKP +EHL++ LHVL+ E
Sbjct: 154 VGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVE 213
Query: 222 FPAEIIDARLMQAREILEDLLKPVDESQDYYKKQQLRELALLNGTLRE 269
+L QA ++ LL P E +D KK+QL ELA++NGT R+
Sbjct: 214 DTENRATVKLAQAVAEVQKLLVPQAEGEDELKKRQLMELAIINGTYRD 261
>GLD1_CAEEL (Q17339) Female germline-specific tumor suppressor gld-1
(Defective in germ line development protein 1)
Length = 463
Score = 123 bits (308), Expect = 6e-28
Identities = 58/135 (42%), Positives = 89/135 (64%)
Query: 139 SGLVVKKTMRVDIPVEVYPNFNFVGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPAKE 198
+G ++ T ++ +P YP++NFVGR+LGPRG + K++E T C++++RG+GS++D +KE
Sbjct: 199 AGDMISITEKIYVPKNEYPDYNFVGRILGPRGMTAKQLEQDTGCKIMVRGKGSMRDKSKE 258
Query: 199 EMMRGKPGYEHLNEPLHVLVEAEFPAEIIDARLMQAREILEDLLKPVDESQDYYKKQQLR 258
RGK +EHL + LHVLV+ E + +L A E ++ LL P E D K++QL
Sbjct: 259 SAHRGKANWEHLEDDLHVLVQCEDTENRVHIKLQAALEQVKKLLIPAPEGTDELKRKQLM 318
Query: 259 ELALLNGTLREEGSP 273
ELA++NGT R SP
Sbjct: 319 ELAIINGTYRPMKSP 333
>YKCA_CAEEL (P42083) Hypothetical protein B0280.11 in chromosome III
Length = 634
Score = 94.7 bits (234), Expect = 2e-19
Identities = 48/126 (38%), Positives = 78/126 (61%), Gaps = 4/126 (3%)
Query: 144 KKTMRVDIPVEVYPNFNFVGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPAKEEMMRG 203
+K +V P E N N VGRL+GPRG +++++E C++ IRG+G KD AKEE +R
Sbjct: 512 EKIDKVFFPPETANNTNPVGRLIGPRGMTIRQLEKDLGCKLFIRGKGCTKDDAKEERLRE 571
Query: 204 KPGYEHLNEPLHVLVEAEFPA-EIIDARLMQAREILEDLLKPVDESQDYYKKQQLRELAL 262
+ G+EHL EP+HV++ + E +L +++L++ L+ D K+ QL +LA+
Sbjct: 572 RVGWEHLKEPIHVMISVRSDSEEAASEKLSSIKKMLQEFLEHTDSE---LKRSQLMQLAV 628
Query: 263 LNGTLR 268
+ GTL+
Sbjct: 629 IEGTLK 634
>SF01_HUMAN (Q15637) Splicing factor 1 (Zinc finger protein 162)
(Transcription factor ZFM1) (Zinc finger gene in MEN1
locus) (Mammalian branch point binding protein mBBP)
(BBP)
Length = 639
Score = 90.5 bits (223), Expect = 4e-18
Identities = 56/125 (44%), Positives = 78/125 (61%), Gaps = 8/125 (6%)
Query: 148 RVDIPVEVYPNFNFVGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPAKEEMMRGK--P 205
+V IP + YP NFVG L+GPRGN+LK +E + +++IRG+GS+K+ K G+ P
Sbjct: 139 KVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKE-GKVGRKDGQMLP 197
Query: 206 GYEHLNEPLHVLVEAEFPAEIIDARLMQAREILEDLLKPVDESQDYYKKQQLRELALLNG 265
G + EPLH LV A E + + Q R IL+ ++ E Q+ +K QLRELA LNG
Sbjct: 198 GED---EPLHALVTAN-TMENVKKAVEQIRNILKQGIE-TPEDQNDLRKMQLRELARLNG 252
Query: 266 TLREE 270
TLRE+
Sbjct: 253 TLRED 257
>SF01_MOUSE (Q64213) Splicing factor 1 (Zinc finger protein 162)
(Transcription factor ZFM1) (mZFM) (Zinc finger gene in
MEN1 locus) (Mammalian branch point binding protein
mBBP) (BBP) (CW17)
Length = 653
Score = 89.0 bits (219), Expect = 1e-17
Identities = 55/126 (43%), Positives = 77/126 (60%), Gaps = 10/126 (7%)
Query: 148 RVDIPVEVYPNFNFVGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPAKEEMMRGKPGY 207
+V IP + YP NFVG L+GPRGN+LK +E + +++IRG+GS+ EE G+
Sbjct: 139 KVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSV-----EEGKVGRKDG 193
Query: 208 EHL---NEPLHVLVEAEFPAEIIDARLMQAREILEDLLKPVDESQDYYKKQQLRELALLN 264
+ L +EPLH LV A E + + Q R IL+ ++ E Q+ +K QLRELA LN
Sbjct: 194 QMLPGEDEPLHALVTAN-TMENVKKAVEQIRNILKQGIE-TPEDQNDLRKMQLRELARLN 251
Query: 265 GTLREE 270
GTLRE+
Sbjct: 252 GTLRED 257
>GRP3_ARTSA (P13230) Glycine-rich protein GRP33
Length = 308
Score = 74.7 bits (182), Expect = 3e-13
Identities = 41/108 (37%), Positives = 67/108 (61%), Gaps = 4/108 (3%)
Query: 143 VKKTMRVDIPVEVYPNFNFVGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPAKEEMMR 202
VK R +PV+ +P +NF+G+LLGP G+++K+++ T ++ I GRGS++D KEE +R
Sbjct: 72 VKLVSRCCLPVDQFPKYNFLGKLLGPGGSTMKQLQDETMTKISILGRGSMRDRNKEEELR 131
Query: 203 --GKPGYEHLNEPLHV-LVEAEFPAEIIDARLMQAREILEDLLKPVDE 247
G Y HLNE LH+ ++ PAE AR+ A ++ + P ++
Sbjct: 132 NSGDVKYAHLNEQLHIEIISIASPAE-AHARMAYALTEIKKYITPEED 178
>YAJE_SCHPO (Q09911) Hypothetical protein C30D11.14c in chromosome I
Length = 534
Score = 39.3 bits (90), Expect = 0.012
Identities = 36/149 (24%), Positives = 68/149 (45%), Gaps = 22/149 (14%)
Query: 114 RFQSEMPSLLQSSATPSWLSPQGSSSG---LVVKKTMRVDIPVEVYPNFNFVGR--LLGP 168
RF+ + +S +P S G+ +G +K + + + + P+ F R ++GP
Sbjct: 253 RFRRREDNERNNSNSPRNFSTHGNGNGENGQPRRKWLEEKVYINLTPSRGFHLRQAIVGP 312
Query: 169 RGNSLKRVEASTDCRVLIRGRGS--IKDPAKEEMMRGKPGYEHLNEPLHVLVEAEFPAEI 226
+G +K ++ T RV I+G+GS I+ E +EP+H+ + + P I
Sbjct: 313 QGAYVKHIQQETRTRVQIKGQGSAFIEPSTNRES----------DEPIHLCIMSHDPNAI 362
Query: 227 IDARLMQAREILEDLLKPVDESQDYYKKQ 255
+A+ + EDL+ V + +K Q
Sbjct: 363 -----QRAKVLCEDLIASVHQQYKAWKSQ 386
>YCF9_YEAST (P25586) Hypothetical 37.2 kDa protein in CHA1-PRD1
intergenic region
Length = 316
Score = 33.5 bits (75), Expect = 0.64
Identities = 13/25 (52%), Positives = 19/25 (76%)
Query: 164 RLLGPRGNSLKRVEASTDCRVLIRG 188
RL+GP GN+LK +E T C +L++G
Sbjct: 144 RLVGPNGNTLKALELLTKCYILVQG 168
>HRB2_MOUSE (Q8BGA5) HIV-1 Rev binding protein 2 homolog
Length = 380
Score = 33.1 bits (74), Expect = 0.84
Identities = 12/25 (48%), Positives = 21/25 (84%)
Query: 164 RLLGPRGNSLKRVEASTDCRVLIRG 188
RL+GP+G++LK +E T+C V+++G
Sbjct: 162 RLIGPKGSTLKALELLTNCYVMVQG 186
>FENR_SYNP2 (P31973) Ferredoxin--NADP reductase (EC 1.18.1.2) (FNR)
Length = 402
Score = 33.1 bits (74), Expect = 0.84
Identities = 29/104 (27%), Positives = 44/104 (41%), Gaps = 27/104 (25%)
Query: 64 YRLINQEILRVTTLLGNASVLGQSRLEHASPLATGGIFSNGGADVNGWASRFQSEMPSLL 123
Y +NQE+ R+T L G ++ E A+ + + G
Sbjct: 51 YARMNQEMQRITKLGGK--IVSIRPAEDAAQIVSEG------------------------ 84
Query: 124 QSSATPSWLSPQGSSSGLVVKKTMRVDIPVEVY-PNFNFVGRLL 166
QSSA S SP SS+ +V KT +PV +Y P F+G+ +
Sbjct: 85 QSSAQASAQSPMASSTKIVHPKTTDTSVPVNIYRPKTPFLGKCI 128
>ECR1_METTH (O26780) Probable exosome complex RNA-binding protein 1
Length = 311
Score = 33.1 bits (74), Expect = 0.84
Identities = 22/87 (25%), Positives = 43/87 (49%), Gaps = 10/87 (11%)
Query: 155 VYPNFNFVGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPAKEEMMRGKPGYEHLNEPL 214
VY V RL+G RG+ + V+ T C +++ G + ++G+P E + E +
Sbjct: 144 VYITPTKVPRLIGKRGSMINMVKEKTHCDIVVGQNGVV-------WIKGEPDMERIAEKV 196
Query: 215 HVLVEAEFPAEIIDARLMQAREILEDL 241
++++ E + R+ RE+L+ L
Sbjct: 197 VLMIDREAHTSGLTDRV---RELLDRL 220
>YG5T_YEAST (P53332) Hypothetical 34.3 kDa protein in TAF145-YOR1
intergenic region
Length = 305
Score = 32.7 bits (73), Expect = 1.1
Identities = 19/55 (34%), Positives = 33/55 (59%), Gaps = 2/55 (3%)
Query: 78 LGNASVLGQSRLEHA-SPLATGGIFSNGGADVNGWASRFQS-EMPSLLQSSATPS 130
+ A + ++R+E SPLA + GG + +GW+ + S E+ LL+SSA+P+
Sbjct: 243 VSGAETVNKTRIEKGMSPLAVHVVNVLGGREEDGWSEKLSSTEIRRLLKSSASPT 297
>HRB2_HUMAN (Q13601) HIV-1 Rev binding protein 2 (Rev interacting
protein 1) (Rip-1)
Length = 381
Score = 32.7 bits (73), Expect = 1.1
Identities = 11/25 (44%), Positives = 21/25 (84%)
Query: 164 RLLGPRGNSLKRVEASTDCRVLIRG 188
RL+GP+G++LK +E T+C ++++G
Sbjct: 163 RLIGPKGSTLKALELLTNCYIMVQG 187
>TBP7_CAEEL (P54816) TAT-binding homolog 7
Length = 1242
Score = 32.3 bits (72), Expect = 1.4
Identities = 16/41 (39%), Positives = 27/41 (65%), Gaps = 3/41 (7%)
Query: 215 HVLVEAEFPAEIIDARLMQAREILEDLLKPV--DESQDYYK 253
HV+ + P ++ D RLM+ R +E ++PV DE++DYY+
Sbjct: 865 HVIEKINTPPKVFDPRLMRDRRFVE-FVEPVDPDEAEDYYE 904
>RA50_AQUAE (O67124) Probable DNA double-strand break repair rad50
ATPase
Length = 978
Score = 32.3 bits (72), Expect = 1.4
Identities = 25/73 (34%), Positives = 38/73 (51%), Gaps = 3/73 (4%)
Query: 198 EEMMRGKPGYEHLNEPLHVLVEAEFPAEIIDARLMQAREILEDLLKPVDESQDYYKKQQL 257
EE+ + P E+L E + L E E ++ +L + R+ LED K +E+Q K Q
Sbjct: 549 EELRKEIP--ENLKERIKKLEELRIEKEKLEHKLNKYRKALEDRQKQKEEAQAKLHKAQ- 605
Query: 258 RELALLNGTLREE 270
EL LL +RE+
Sbjct: 606 TELELLKEKIREK 618
>CIRH_HUMAN (Q969X6) Cirhin
Length = 686
Score = 32.0 bits (71), Expect = 1.9
Identities = 41/152 (26%), Positives = 63/152 (40%), Gaps = 27/152 (17%)
Query: 118 EMPSLLQSSATPSWLSPQGSSSGLVV--KKTMRVDIPVEVYPNFNFVGRLLGPRGNSLKR 175
E LL S +WL+ G+S+G+ V K +++ V Y NF + P N+L
Sbjct: 485 EAMCLLAVSPDGNWLAASGTSAGVHVYNVKQLKLHCTVPAY-NFPVTAMAIAPNTNNL-- 541
Query: 176 VEASTDCRVLIRGRGSIKDPAKEEMMR--GKPGYEHL---------------NEPLHVLV 218
V A +D +V SI D + R K G+ HL P+H+L+
Sbjct: 542 VIAHSDQQVF---EYSIPDKQYTDWSRTVQKQGFHHLWLQRDTPITHISFHPKRPMHILL 598
Query: 219 EAEFPAEIIDAR--LMQAREILEDLLKPVDES 248
+ IID L + +L + P +ES
Sbjct: 599 HDAYMFCIIDKSLPLPNDKTLLYNPFPPTNES 630
>CIRH_MOUSE (Q8R2N2) Cirhin (Testis expressed gene 292 protein)
(Fragment)
Length = 540
Score = 31.6 bits (70), Expect = 2.4
Identities = 40/156 (25%), Positives = 63/156 (39%), Gaps = 23/156 (14%)
Query: 118 EMPSLLQSSATPSWLSPQGSSSGLVVKKTMRVDIPVEVYPNFNFVGRLLGPRGNSLKRVE 177
E LL S +WL+ G+S+G+ V + + V P +NF L N+ V
Sbjct: 339 EAMCLLAVSPDGNWLAASGTSAGVHVYDLHHLKLHCTV-PAYNFPVTALAIAPNTNNLVI 397
Query: 178 ASTDCRVLIRGRGSIKDPAKEEMMRG--KPGYE---------------HLNEPLHVLVEA 220
A +D +V SI + E R K G+ H P+H+L+
Sbjct: 398 AHSDQQVF---EFSIPEKQYTEWSRSLQKQGFHQLWLQRDTPITHISFHPKRPMHILLHD 454
Query: 221 EFPAEIIDARLMQARE--ILEDLLKPVDESQDYYKK 254
+ IID L E +L + L P +ES + ++
Sbjct: 455 AYMFCIIDKSLPLPNEKTVLYNPLPPKNESDVFLRR 490
>RECN_HELPY (O25943) DNA repair protein recN (Recombination protein
N)
Length = 524
Score = 31.2 bits (69), Expect = 3.2
Identities = 19/84 (22%), Positives = 41/84 (48%)
Query: 191 SIKDPAKEEMMRGKPGYEHLNEPLHVLVEAEFPAEIIDARLMQAREILEDLLKPVDESQD 250
S K+ +++ E+ ++ H L AE + + L++A +LE ++E +
Sbjct: 219 SSKEKLNDKIALALEVLENTHKITHALESVGHSAEFLKSALLEASALLEKEQAKLEECER 278
Query: 251 YYKKQQLRELALLNGTLREEGSPM 274
++ L L +L+G +++ GS M
Sbjct: 279 LDIEKVLERLGMLSGIIKDYGSIM 302
>S160_YEAST (P06105) SCP160 protein (Protein HX)
Length = 1222
Score = 30.4 bits (67), Expect = 5.4
Identities = 27/104 (25%), Positives = 47/104 (44%), Gaps = 10/104 (9%)
Query: 144 KKTMRVDIPVEVYPNFNFVGRLLGPRGNSLKRVEASTDCRVLI---RGRGSIKDPAKEEM 200
K M V+IP N V RL+G +G++L+++ C++ I + KD E
Sbjct: 634 KSKMTVNIPA------NSVARLIGNKGSNLQQIREKFACQIDIPNEENNNASKDKTVEVT 687
Query: 201 MRGKPGYEHLNEPLHVLVEAEFPAEIIDARLMQAREILEDLLKP 244
+ G Y + ++ EA+ A+II L+ + L+ P
Sbjct: 688 LTGLE-YNLTHAKKYLAAEAKKWADIITKELIVPVKFHGSLIGP 730
>RECN_HELPJ (Q9ZJ80) DNA repair protein recN (Recombination protein
N)
Length = 522
Score = 30.4 bits (67), Expect = 5.4
Identities = 18/82 (21%), Positives = 41/82 (49%)
Query: 191 SIKDPAKEEMMRGKPGYEHLNEPLHVLVEAEFPAEIIDARLMQAREILEDLLKPVDESQD 250
S K+ +++ E+ ++ H L AE + + L++A +LE ++E +
Sbjct: 219 SSKEKLNDKIALALDVLENTHKITHALESVGHSAEFLKSALLEAGALLEKEQAKLEECER 278
Query: 251 YYKKQQLRELALLNGTLREEGS 272
++ L +L +L+G +++ GS
Sbjct: 279 LDIEKVLEKLGMLSGIIKDYGS 300
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,334,537
Number of Sequences: 164201
Number of extensions: 1526380
Number of successful extensions: 5453
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 5429
Number of HSP's gapped (non-prelim): 32
length of query: 294
length of database: 59,974,054
effective HSP length: 109
effective length of query: 185
effective length of database: 42,076,145
effective search space: 7784086825
effective search space used: 7784086825
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0083.16