BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0079a.1
         (139 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

RBM5_HUMAN (P52756) RNA-binding protein 5 (RNA binding motif pro...    47  1e-05
VG5Q_HUMAN (Q8N302) Angiogenic factor VG5Q (Vasculogenesis gene ...    42  3e-04
VG5Q_MOUSE (Q7TN31) Angiogenic factor VG5Q (mVG5Q)                     40  0.002
RBMA_RAT (P70501) RNA-binding protein 10 (RNA binding motif prot...    39  0.003
RBMA_HUMAN (P98175) RNA-binding protein 10 (RNA binding motif pr...    39  0.003
RBM6_HUMAN (P78332) RNA-binding protein 6 (RNA binding motif pro...    34  0.11
YNJ1_YEAST (P53935) Hypothetical 141.5 kDa protein in YPT53-RHO2...    33  0.19
YH57_SCHPO (O94312) PWWP domain protein C215.07c                       33  0.25
YP23_CAEEL (Q09202) Hypothetical protein AH6.3 in chromosome II        32  0.32
RA23_YEAST (P32628) UV excision repair protein RAD23                   32  0.42
PPZ2_YEAST (P33329) Serine/threonine protein phosphatase PP-Z2 (...    32  0.42
HXB3_CHICK (P23682) Homeobox protein Hox-B3 (Chox-2.7)                 32  0.42
MR15_DROME (Q9Y0I1) MRG15 protein                                      32  0.55
CBF5_ASPFU (O43102) Centromere/microtubule binding protein CBF5 ...    32  0.55
TCOF_MOUSE (O08784) Treacle protein (Treacher Collins syndrome p...    31  0.72
EFG1_CANAL (P43064) Enhanced filamentous growth protein                31  0.93
DAH1_MOUSE (Q9QYB2) Dachshund homolog 1 (Dach1)                        31  0.93
YAP4_YEAST (P40917) AP-1 like transcription factor YAP4 (Transcr...    30  1.2
T421_ECOLI (P11901) Transposase for insertion sequence element I...    30  1.2
INSL_ECOLI (P08409) Putative transposase insL for insertion sequ...    30  1.2

>RBM5_HUMAN (P52756) RNA-binding protein 5 (RNA binding motif
           protein 5) (Putative tumor suppressor LUCA15) (G15
           protein)
          Length = 815

 Score = 47.0 bits (110), Expect = 1e-05
 Identities = 23/72 (31%), Positives = 37/72 (50%), Gaps = 4/72 (5%)

Query: 1   WDFESSSGYYYHKTNGFYYDPKSGFYYSDAIGK---WVTQEEAYASPHFASTTKKASSTS 57
           + ++ SSGYYY  T G YYDP S +YY+    +   W  ++E Y  P   S++ + S   
Sbjct: 462 YQYDESSGYYYDPTTGLYYDPNSQYYYNSLTQQYLYWDGEKETYV-PAAESSSHQQSGLP 520

Query: 58  QSNKGNKPHNGP 69
            + +G +    P
Sbjct: 521 PAKEGKEKKEKP 532



 Score = 36.2 bits (82), Expect = 0.022
 Identities = 20/63 (31%), Positives = 30/63 (46%), Gaps = 3/63 (4%)

Query: 6   SSGYYYHKTNGFYYDPKSGFYY---SDAIGKWVTQEEAYASPHFASTTKKASSTSQSNKG 62
           +S Y Y +++G+YYDP +G YY   S      +TQ+  Y      +    A S+S    G
Sbjct: 459 TSTYQYDESSGYYYDPTTGLYYDPNSQYYYNSLTQQYLYWDGEKETYVPAAESSSHQQSG 518

Query: 63  NKP 65
             P
Sbjct: 519 LPP 521


>VG5Q_HUMAN (Q8N302) Angiogenic factor VG5Q (Vasculogenesis gene on
           5q) (hVG5Q)
          Length = 714

 Score = 42.4 bits (98), Expect = 3e-04
 Identities = 20/65 (30%), Positives = 31/65 (46%), Gaps = 2/65 (3%)

Query: 3   FESSSGYYYHKTNGFYYDPKSGFYYSDAI--GKWVTQEEAYASPHFASTTKKASSTSQSN 60
           F+ S+G+YY   N  YYDP +G YY   +  G++         P+  S+TK++       
Sbjct: 217 FDHSTGFYYDSENQLYYDPSTGIYYYCDVESGRYQFHSRVDLQPYPTSSTKQSKDKKLKK 276

Query: 61  KGNKP 65
           K   P
Sbjct: 277 KRKDP 281



 Score = 30.0 bits (66), Expect = 1.6
 Identities = 10/23 (43%), Positives = 15/23 (64%)

Query: 5   SSSGYYYHKTNGFYYDPKSGFYY 27
           S +G+ Y +  G Y+D  +GFYY
Sbjct: 203 SQTGFSYDENTGLYFDHSTGFYY 225


>VG5Q_MOUSE (Q7TN31) Angiogenic factor VG5Q (mVG5Q)
          Length = 711

 Score = 39.7 bits (91), Expect = 0.002
 Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 2/51 (3%)

Query: 3   FESSSGYYYHKTNGFYYDPKSGFYYSDAI--GKWVTQEEAYASPHFASTTK 51
           F+ S+G+YY   N  YYDP +G YY   +  G++         P+  S+TK
Sbjct: 217 FDHSTGFYYDSENQLYYDPSTGIYYYCDVESGRYQFHSRVDLQPYQTSSTK 267



 Score = 33.5 bits (75), Expect = 0.14
 Identities = 16/62 (25%), Positives = 28/62 (44%)

Query: 3   FESSSGYYYHKTNGFYYDPKSGFYYSDAIGKWVTQEEAYASPHFASTTKKASSTSQSNKG 62
           ++ S+G Y+  + GFYYD ++  YY  + G +   +       F S        + S K 
Sbjct: 209 YDESTGLYFDHSTGFYYDSENQLYYDPSTGIYYYCDVESGRYQFHSRVDLQPYQTSSTKP 268

Query: 63  NK 64
           N+
Sbjct: 269 NR 270



 Score = 30.4 bits (67), Expect = 1.2
 Identities = 10/23 (43%), Positives = 16/23 (69%)

Query: 5   SSSGYYYHKTNGFYYDPKSGFYY 27
           S +G+ Y ++ G Y+D  +GFYY
Sbjct: 203 SQTGFTYDESTGLYFDHSTGFYY 225


>RBMA_RAT (P70501) RNA-binding protein 10 (RNA binding motif protein
           10) (S1-1 protein)
          Length = 852

 Score = 39.3 bits (90), Expect = 0.003
 Identities = 22/69 (31%), Positives = 31/69 (44%), Gaps = 5/69 (7%)

Query: 1   WDFESSSGYYYHKTNGFYYDPKSGFYY---SDAIGKWVTQEEAY--ASPHFASTTKKASS 55
           + ++ +SGYYY    G YYDP S +YY   S     W  +   Y  A    A   K   +
Sbjct: 494 YQYDETSGYYYDPQTGLYYDPNSQYYYNAQSQQYLYWDGERRTYIPALEQSADGHKDTGA 553

Query: 56  TSQSNKGNK 64
           +S+  K  K
Sbjct: 554 SSKEGKEKK 562



 Score = 35.0 bits (79), Expect = 0.050
 Identities = 12/21 (57%), Positives = 17/21 (80%)

Query: 7   SGYYYHKTNGFYYDPKSGFYY 27
           S Y Y +T+G+YYDP++G YY
Sbjct: 492 STYQYDETSGYYYDPQTGLYY 512


>RBMA_HUMAN (P98175) RNA-binding protein 10 (RNA binding motif
           protein 10) (DXS8237E)
          Length = 929

 Score = 39.3 bits (90), Expect = 0.003
 Identities = 22/69 (31%), Positives = 31/69 (44%), Gaps = 5/69 (7%)

Query: 1   WDFESSSGYYYHKTNGFYYDPKSGFYY---SDAIGKWVTQEEAY--ASPHFASTTKKASS 55
           + ++ +SGYYY    G YYDP S +YY   S     W  +   Y  A    A   K+  +
Sbjct: 571 YQYDETSGYYYDPQTGLYYDPNSQYYYNAQSQQYLYWDGERRTYVPALEQSADGHKETGA 630

Query: 56  TSQSNKGNK 64
            S+  K  K
Sbjct: 631 PSKEGKEKK 639



 Score = 35.0 bits (79), Expect = 0.050
 Identities = 12/21 (57%), Positives = 17/21 (80%)

Query: 7   SGYYYHKTNGFYYDPKSGFYY 27
           S Y Y +T+G+YYDP++G YY
Sbjct: 569 STYQYDETSGYYYDPQTGLYY 589


>RBM6_HUMAN (P78332) RNA-binding protein 6 (RNA binding motif
           protein 6) (RNA-binding protein DEF-3) (Lung cancer
           antigen NY-LU-12) (Protein G16)
          Length = 1123

 Score = 33.9 bits (76), Expect = 0.11
 Identities = 22/73 (30%), Positives = 28/73 (38%), Gaps = 9/73 (12%)

Query: 5   SSSGYYYHKTNGFYYDPKSGFYYSDAIGKWVTQEEAYASPH----FASTTKKASSTSQSN 60
           SS  Y Y    G+YYDP +G YY        TQ+E Y             K+   TSQ  
Sbjct: 786 SSDCYIYDSATGYYYDPLAGTYYDPN-----TQQEVYVPQDPGLPEEEEIKEKKPTSQGK 840

Query: 61  KGNKPHNGPLPGR 73
             +K       G+
Sbjct: 841 SSSKKEMSKRDGK 853



 Score = 30.4 bits (67), Expect = 1.2
 Identities = 17/62 (27%), Positives = 26/62 (41%)

Query: 3   FESSSGYYYHKTNGFYYDPKSGFYYSDAIGKWVTQEEAYASPHFASTTKKASSTSQSNKG 62
           ++S++GYYY    G YYDP +           + +EE        S  K +S    S + 
Sbjct: 792 YDSATGYYYDPLAGTYYDPNTQQEVYVPQDPGLPEEEEIKEKKPTSQGKSSSKKEMSKRD 851

Query: 63  NK 64
            K
Sbjct: 852 GK 853


>YNJ1_YEAST (P53935) Hypothetical 141.5 kDa protein in YPT53-RHO2
           intergenic region
          Length = 1240

 Score = 33.1 bits (74), Expect = 0.19
 Identities = 15/35 (42%), Positives = 24/35 (67%)

Query: 96  KRKRPGEKPKVVSEEEKSALKAREAARKRVQEREK 130
           KRKR  EK ++  E E+  ++ REA RK+V+E ++
Sbjct: 667 KRKREEEKERLKKELEEREMRRREAQRKKVEEAKR 701


>YH57_SCHPO (O94312) PWWP domain protein C215.07c
          Length = 568

 Score = 32.7 bits (73), Expect = 0.25
 Identities = 26/86 (30%), Positives = 34/86 (39%), Gaps = 12/86 (13%)

Query: 53  ASSTSQSNKGNKPHNGPLPGRVVTTSLNPTRNVKAAPSSLSLGKRKRPGEKPKVVSEEEK 112
           ASS   SN   K            T ++P R+  A+       KR    E  K   EEE 
Sbjct: 283 ASSNVSSNPAKK------------TRVSPRRSTAASKKKSPSSKRASSDEIEKDKEEEEG 330

Query: 113 SALKAREAARKRVQEREKSLLGLYSK 138
           S     + A++    RE+SLL L  K
Sbjct: 331 SVANEEDVAKRTFHSREQSLLFLRHK 356


>YP23_CAEEL (Q09202) Hypothetical protein AH6.3 in chromosome II
          Length = 230

 Score = 32.3 bits (72), Expect = 0.32
 Identities = 21/83 (25%), Positives = 36/83 (43%), Gaps = 5/83 (6%)

Query: 54  SSTSQSNKGNKPHNGPLPGRVVTTSLNPTRNVKAAPSSL-----SLGKRKRPGEKPKVVS 108
           +++ +SNK  KP  G    +    S       K            L + ++P  K K   
Sbjct: 85  ANSKKSNKSKKPEKGSKKSKKSEKSKKKKTEEKVMSEDKPERPDKLTEEEKPDAKEKKEI 144

Query: 109 EEEKSALKAREAARKRVQEREKS 131
           E+EK+  K +   +++ QE+EKS
Sbjct: 145 EKEKTKEKEKTKEKEKTQEKEKS 167


>RA23_YEAST (P32628) UV excision repair protein RAD23
          Length = 398

 Score = 32.0 bits (71), Expect = 0.42
 Identities = 18/69 (26%), Positives = 35/69 (50%), Gaps = 1/69 (1%)

Query: 46  FASTTKKASSTSQSNKGNKPHNGPLPGRVVTTSLNPTRNVKAAPSSLSLGKRKRPGEKPK 105
           F  + KK++ T  +     P +   PGR  +T  +P+ +  AAP++ +  +  +P E+  
Sbjct: 70  FMVSQKKSTKTKVTEPPIAPESATTPGRENSTEASPSTDASAAPAA-TAPEGSQPQEEQT 128

Query: 106 VVSEEEKSA 114
             +E  +SA
Sbjct: 129 ATTERTESA 137


>PPZ2_YEAST (P33329) Serine/threonine protein phosphatase PP-Z2 (EC
           3.1.3.16)
          Length = 709

 Score = 32.0 bits (71), Expect = 0.42
 Identities = 30/115 (26%), Positives = 49/115 (42%), Gaps = 16/115 (13%)

Query: 5   SSSGYYYHKTNGFYYDPKSGFYYSDAIGKWVTQEEAYASPHFASTTKKASSTSQSNKGNK 64
           + S Y+ +++N      KS F          T   AY++P  +   +K SS    + G+ 
Sbjct: 240 NGSDYFSNRSNSHASSRKSSF--------GSTGNTAYSTPLHSPALRKMSSRDNDDSGDN 291

Query: 65  PHNGPLPGRVVTTSLNPTRNV-KAAPSSLSLGKRKRPGEKPKVVSEEEKSALKAR 118
            +     GR   TS  P  N+ K +PS+ S  KR+     P +   +  S+L  R
Sbjct: 292 VN-----GR--GTSPIPNLNIDKPSPSASSASKREYLSAYPTLAHRDSSSSLSPR 339


>HXB3_CHICK (P23682) Homeobox protein Hox-B3 (Chox-2.7)
          Length = 399

 Score = 32.0 bits (71), Expect = 0.42
 Identities = 31/110 (28%), Positives = 46/110 (41%), Gaps = 6/110 (5%)

Query: 3   FESSS---GYYYHKTNGFYYDPKSGFYYSDAIGKWVTQEEAYASPHFASTTKKASSTSQS 59
           +ESS+   GY Y  TNGF Y+     +   +  +   Q  A +     +TT+ A S   +
Sbjct: 7   YESSTLFGGYSYGSTNGFGYEGPQQPFQPGSHVENDFQRSACSLQSLGNTTQHAKSKDLN 66

Query: 60  NKGNKPH--NGPLPGRVVTTSLNPTRNVKAAPSSLSLGKRKRPGEKPKVV 107
               +P       P   V+   NPT N   + +S+  G  K P  KP  V
Sbjct: 67  GSCMRPSLPQEHHPPPTVSPPPNPTTN-STSSNSIPSGSAKVPRVKPTSV 115


>MR15_DROME (Q9Y0I1) MRG15 protein
          Length = 424

 Score = 31.6 bits (70), Expect = 0.55
 Identities = 30/104 (28%), Positives = 41/104 (38%), Gaps = 18/104 (17%)

Query: 47  ASTTKKASSTSQSNKGNKPHNGPLP------GRVVTTSLNPTRN----------VKAAPS 90
           AST  K S+TSQS   + P     P      G   TT+ N T N           ++ PS
Sbjct: 118 ASTPSKDSNTSQSTASSTPTTSAGPGSKSEAGSTGTTTTNSTANSTTSRAHRKSTQSTPS 177

Query: 91  SLSLGKRKRPGEKPKVVSEEEKSALKAREAARKRVQEREKSLLG 134
           +   G      E P      E+    A +  +KR+ E+  SL G
Sbjct: 178 TARPGTPSDKKEDPAAAETTEEEGPVAPK--KKRMSEQRPSLTG 219


>CBF5_ASPFU (O43102) Centromere/microtubule binding protein CBF5
           (Centromere-binding factor 5) (Small nucleolar RNP
           protein CBF5) (H/ACA snoRNP protein CBF5)
          Length = 487

 Score = 31.6 bits (70), Expect = 0.55
 Identities = 23/110 (20%), Positives = 48/110 (42%), Gaps = 10/110 (9%)

Query: 22  KSGFYYSDAIGKWVTQEEAYASPHFASTTKKASSTSQSNKGNKPHNGPLPGRVVTTSLNP 81
           K G    D   KW T+ + Y++P  AS    + ST++ +              +TT  + 
Sbjct: 377 KYGRANEDTPAKWKTEYKDYSAPEEASAHAASESTAKEDVA----------AALTTEQDE 426

Query: 82  TRNVKAAPSSLSLGKRKRPGEKPKVVSEEEKSALKAREAARKRVQEREKS 131
             +   +   +   K ++  ++ +  + EE++  K ++  +K  +ER KS
Sbjct: 427 APSSPQSKMDVDETKEEKKRKRHEGETAEERAERKRKKKEKKEKKERRKS 476


>TCOF_MOUSE (O08784) Treacle protein (Treacher Collins syndrome
            protein homolog)
          Length = 1320

 Score = 31.2 bits (69), Expect = 0.72
 Identities = 27/93 (29%), Positives = 41/93 (44%), Gaps = 14/93 (15%)

Query: 43   SPHFASTTKKASST-SQSNKGNKPHNGPLPGRVVTTSLNPTRNVKAAPSSLSLGKRKRPG 101
            SP  A  T KA S   + + G K    P P           +  K  P    LG +   G
Sbjct: 1216 SPEKAPMTSKAKSKLDKGSAGGKGKGSPGP-----------QGAKEKPDGELLGIKLESG 1264

Query: 102  EK--PKVVSEEEKSALKAREAARKRVQEREKSL 132
            E+  PK  S+++KS  K ++  +K  ++ +KSL
Sbjct: 1265 EQSDPKSKSKKKKSLKKKKDKEKKEKKKGKKSL 1297


>EFG1_CANAL (P43064) Enhanced filamentous growth protein
          Length = 552

 Score = 30.8 bits (68), Expect = 0.93
 Identities = 32/123 (26%), Positives = 56/123 (45%), Gaps = 19/123 (15%)

Query: 9   YYYHKTNGFYYDPKS--GFYYSDAIGKWVTQEEAY----ASPHFASTTKKASSTSQSNKG 62
           Y Y+  N + Y   +  G YY   I   ++Q +      ++ ++ S    A   S S  G
Sbjct: 94  YDYNTYNRYQYPAATSQGNYYQQTIPNQLSQPQPQHYNGSNRNYTSAPSGAPIPSNSTSG 153

Query: 63  NKPHNGPLPGRV-------VTTSLNPT--RNVKAAPSSLSLGKRKRPGEKPKVVS---EE 110
                 PLPG+        V+T   PT  ++   A S+ ++G+ + PG +P+V +   E+
Sbjct: 154 PS-QQPPLPGQQAVPIPPHVSTMQQPTPVQDTLNASSTSTVGQFQPPGIRPRVTTTMWED 212

Query: 111 EKS 113
           EK+
Sbjct: 213 EKT 215


>DAH1_MOUSE (Q9QYB2) Dachshund homolog 1 (Dach1)
          Length = 751

 Score = 30.8 bits (68), Expect = 0.93
 Identities = 14/43 (32%), Positives = 26/43 (59%)

Query: 42  ASPHFASTTKKASSTSQSNKGNKPHNGPLPGRVVTTSLNPTRN 84
           +S   +S++  +SS+S S+  +    GPLPG+ V ++ +P  N
Sbjct: 132 SSSSSSSSSSSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVEN 174


>YAP4_YEAST (P40917) AP-1 like transcription factor YAP4
           (Transcription activator CIN5) (Chromosome instability
           protein 5)
          Length = 295

 Score = 30.4 bits (67), Expect = 1.2
 Identities = 37/140 (26%), Positives = 64/140 (45%), Gaps = 16/140 (11%)

Query: 3   FESSSGYYYHKTNGFYYDPKSGFYYSDAIGKWVTQEEAYASPHFASTTKKASSTSQS--- 59
           FESS    Y   + F   P + ++ S++     T++ + A+ +  S  ++  S S S   
Sbjct: 165 FESS----YTTASTFTSQPAASYFPSNSTP--ATRKNS-ATTNLPSEERRRVSVSLSEQV 217

Query: 60  -NKGNKPHNGPLPGRVVTTSLNPTRNVKAAPSSLSLGKRKRPGEKPKVVSEEEKSALKAR 118
            N+G + +N    G+++  +  P RN K A  + S  K  R   +  + + EEKS  K  
Sbjct: 218 FNEGERYNND---GQLIGKTGKPLRNTKRAAQNRSAQKAFRQRREKYIKNLEEKS--KLF 272

Query: 119 EAARKRVQEREKSLLGLYSK 138
           +   K   E +K +  L SK
Sbjct: 273 DGLMKENSELKKMIESLKSK 292


>T421_ECOLI (P11901) Transposase for insertion sequence element
           IS421
          Length = 371

 Score = 30.4 bits (67), Expect = 1.2
 Identities = 27/93 (29%), Positives = 40/93 (42%), Gaps = 17/93 (18%)

Query: 60  NKGNKPHNGPLPGRVVTTSLNPTRNVKAAPSSLSLGKRKRPGEKPKVVS----------- 108
           N GNK    P P R++  SL P + + +    LS  +RK    + + +            
Sbjct: 233 NSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQAETLEAAGHVLLLTSL 292

Query: 109 -EEEKSALKAREAARKRVQ-----EREKSLLGL 135
            E+E SA +  +  R R Q     +R KSLL L
Sbjct: 293 PEDEYSAEQVADCYRLRWQIELAFKRLKSLLHL 325


>INSL_ECOLI (P08409) Putative transposase insL for insertion
           sequence element IS186A/B/C
          Length = 370

 Score = 30.4 bits (67), Expect = 1.2
 Identities = 27/93 (29%), Positives = 40/93 (42%), Gaps = 17/93 (18%)

Query: 60  NKGNKPHNGPLPGRVVTTSLNPTRNVKAAPSSLSLGKRKRPGEKPKVVS----------- 108
           N GNK    P P R++  SL P + + +    LS  +RK    + + +            
Sbjct: 232 NSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQAETLEAAGHVLLLTSL 291

Query: 109 -EEEKSALKAREAARKRVQ-----EREKSLLGL 135
            E+E SA +  +  R R Q     +R KSLL L
Sbjct: 292 PEDEYSAEQVADCYRLRWQIELAFKRLKSLLHL 324


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.306    0.125    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,152,817
Number of Sequences: 164201
Number of extensions: 669901
Number of successful extensions: 2815
Number of sequences better than 10.0: 81
Number of HSP's better than 10.0 without gapping: 31
Number of HSP's successfully gapped in prelim test: 51
Number of HSP's that attempted gapping in prelim test: 2713
Number of HSP's gapped (non-prelim): 145
length of query: 139
length of database: 59,974,054
effective HSP length: 99
effective length of query: 40
effective length of database: 43,718,155
effective search space: 1748726200
effective search space used: 1748726200
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 60 (27.7 bits)