BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0074.7
(141 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HIC2_MOUSE (Q9JLZ6) Hypermethylated in cancer 2 protein (Hic-2) 32 0.34
ENAM_MOUSE (O55196) Enamelin precursor 32 0.34
VGLE_HHV11 (P04488) Glycoprotein E precursor 32 0.58
SYP2_HUMAN (Q9UMS6) Synaptopodin-2 (Myopodin) (Genethonin 2) 31 0.75
RPB1_MOUSE (P08775) DNA-directed RNA polymerase II largest subun... 31 0.75
RPB1_HUMAN (P24928) DNA-directed RNA polymerase II largest subun... 31 0.75
RPB1_CRIGR (P11414) DNA-directed RNA polymerase II largest subun... 31 0.75
PROS_DROME (P29617) Protein prospero 31 0.75
PPZ_SCHPO (P78968) Serine/threonine protein phosphatase PP-Z (EC... 31 0.75
ZAP3_HUMAN (P49750) Nuclear protein ZAP3 (ZAP113) 31 0.98
HMFT_DROHY (P48590) Segmentation protein fushi tarazu 31 0.98
APXL_HUMAN (Q13796) Apical-like protein (APXL protein) 31 0.98
TOF2_YEAST (Q02208) Topoisomerase 1-associated factor 2 30 1.3
SMA1_COTJA (Q9I962) Mothers against decapentaplegic homolog 1 (S... 30 1.3
RPB1_CAEEL (P16356) DNA-directed RNA polymerase II largest subun... 30 1.3
KGP3_DROME (P32023) cGMP-dependent protein kinase, isozyme 2 for... 30 1.3
ATXL_MOUSE (Q7TQH0) Ataxin-2-like protein 30 1.3
RI15_YEAST (P43565) Serine/threonine-protein kinase RIM15 (EC 2.... 30 1.7
PAD3_RAT (Q9Z340) Partitioning-defective 3 homolog (PARD-3) (PAR... 30 1.7
NFC2_HUMAN (Q13469) Nuclear factor of activated T-cells, cytopla... 30 1.7
>HIC2_MOUSE (Q9JLZ6) Hypermethylated in cancer 2 protein (Hic-2)
Length = 619
Score = 32.3 bits (72), Expect = 0.34
Identities = 16/50 (32%), Positives = 24/50 (48%)
Query: 80 KEDPSVPPWMSPPQYYNKDPSSKSSSYYSAEDPTSPKEYPKVESSSYAQL 129
K+ P +PP P +DP+ S S + PTS P S+S+ +L
Sbjct: 250 KKSPPLPPTTPGPHLTPEDPAQLSDSQRESPAPTSTSALPVGNSASFVEL 299
>ENAM_MOUSE (O55196) Enamelin precursor
Length = 1274
Score = 32.3 bits (72), Expect = 0.34
Identities = 23/87 (26%), Positives = 33/87 (37%), Gaps = 1/87 (1%)
Query: 31 PSLPKDPKHNNQGVRHEYCDQQAVIHSTEGTIRVSPDRKHFALYYPEDPKEDPSVPPWMS 90
P L +D ++ +RH + I E T ++ + P D K PS
Sbjct: 713 PPLKEDYGRQDENLRHPSYGSRGNIFYHEYTNPYHNEKSQYIKSNPWD-KSSPSTMMRPE 771
Query: 91 PPQYYNKDPSSKSSSYYSAEDPTSPKE 117
PQY K + Y+ EDP P E
Sbjct: 772 NPQYTMTSLDQKETEQYNEEDPIDPNE 798
>VGLE_HHV11 (P04488) Glycoprotein E precursor
Length = 550
Score = 31.6 bits (70), Expect = 0.58
Identities = 18/78 (23%), Positives = 32/78 (40%), Gaps = 18/78 (23%)
Query: 75 YPEDPKEDPSVPPWMSPPQYYNKDPSSKSSSYYSAEDPTSPKEYPKVESSS--------- 125
+ D + + PW++PP+ D S + S + PT+P YP+ +
Sbjct: 475 WSSDSEGERDQVPWLAPPE--RPDSPSTNGSGFEILSPTAPSVYPRSDGHQSRRQLTTFG 532
Query: 126 -------YAQLHNSSKYW 136
Y+Q +SS +W
Sbjct: 533 SGRPDRRYSQASDSSVFW 550
>SYP2_HUMAN (Q9UMS6) Synaptopodin-2 (Myopodin) (Genethonin 2)
Length = 1093
Score = 31.2 bits (69), Expect = 0.75
Identities = 18/43 (41%), Positives = 25/43 (57%), Gaps = 2/43 (4%)
Query: 84 SVPPWMSPPQYYNKDPSSKSSSYYSAEDPTSPKEYPKVESSSY 126
SVP + SPP ++ + S S+S PTSPK+ + SSSY
Sbjct: 1026 SVPAYTSPPSFFAEASSPVSASPVPVGIPTSPKQ--ESASSSY 1066
>RPB1_MOUSE (P08775) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) (RPB1)
Length = 1970
Score = 31.2 bits (69), Expect = 0.75
Identities = 21/64 (32%), Positives = 27/64 (41%), Gaps = 2/64 (3%)
Query: 74 YYPEDPKEDPSVPPWM-SPPQYYNKDPS-SKSSSYYSAEDPTSPKEYPKVESSSYAQLHN 131
Y P P P+ P + + P Y PS S SS Y+ + PT P SS +
Sbjct: 1776 YTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPT 1835
Query: 132 SSKY 135
S KY
Sbjct: 1836 SPKY 1839
>RPB1_HUMAN (P24928) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) (RPB1)
Length = 1970
Score = 31.2 bits (69), Expect = 0.75
Identities = 21/64 (32%), Positives = 27/64 (41%), Gaps = 2/64 (3%)
Query: 74 YYPEDPKEDPSVPPWM-SPPQYYNKDPS-SKSSSYYSAEDPTSPKEYPKVESSSYAQLHN 131
Y P P P+ P + + P Y PS S SS Y+ + PT P SS +
Sbjct: 1776 YTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPT 1835
Query: 132 SSKY 135
S KY
Sbjct: 1836 SPKY 1839
>RPB1_CRIGR (P11414) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) (RPB1) (Fragment)
Length = 467
Score = 31.2 bits (69), Expect = 0.75
Identities = 21/64 (32%), Positives = 27/64 (41%), Gaps = 2/64 (3%)
Query: 74 YYPEDPKEDPSVPPWM-SPPQYYNKDPS-SKSSSYYSAEDPTSPKEYPKVESSSYAQLHN 131
Y P P P+ P + + P Y PS S SS Y+ + PT P SS +
Sbjct: 273 YTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPT 332
Query: 132 SSKY 135
S KY
Sbjct: 333 SPKY 336
>PROS_DROME (P29617) Protein prospero
Length = 1403
Score = 31.2 bits (69), Expect = 0.75
Identities = 30/113 (26%), Positives = 45/113 (39%), Gaps = 9/113 (7%)
Query: 31 PSLPKDPKHNNQGVRHEYCDQQAVIHS---TEGTIRVSPDRKHF-ALYYPEDPKEDPSVP 86
PS P+ + Q + + QQ + + T SP R A Y+P+ P P +
Sbjct: 1025 PSTPQQQQQQQQQQQQQQQQQQQQASNGGNSNATPAQSPTRSSGGAAYHPQPPPPPPPMM 1084
Query: 87 PWMSPPQYYNKDPSSKSSSYYSAEDPTSPKEYPKVES--SSYAQLHNSSKYWH 137
P P +PS S +S P SP P + ++ AQLH + H
Sbjct: 1085 PVSLPTSVAIPNPSLHESKVFS---PYSPFFNPHAAAGQATAAQLHQHHQQHH 1134
>PPZ_SCHPO (P78968) Serine/threonine protein phosphatase PP-Z (EC
3.1.3.16)
Length = 515
Score = 31.2 bits (69), Expect = 0.75
Identities = 28/90 (31%), Positives = 36/90 (39%), Gaps = 18/90 (20%)
Query: 63 RVSPDRKHFALYYPEDPKEDPSVPPWMSP------PQYYNKDPSSKSSSYYSAEDPTSPK 116
R S D H YPE P PS+PP SP P K S + S++ PTSP
Sbjct: 49 RTSTDTTHSRHRYPETP---PSLPPPPSPGILATSPAVLQKHQQEDSGN--SSQSPTSPH 103
Query: 117 E-------YPKVESSSYAQLHNSSKYWHYS 139
P +S + H+SS + S
Sbjct: 104 PSNQPAMLSPSTAASQHHHHHSSSSSYAVS 133
>ZAP3_HUMAN (P49750) Nuclear protein ZAP3 (ZAP113)
Length = 1822
Score = 30.8 bits (68), Expect = 0.98
Identities = 22/61 (36%), Positives = 25/61 (40%), Gaps = 6/61 (9%)
Query: 77 EDPKEDPSVPP--WMSPPQYYNKDPSSKSSSYYSAEDPTSPKEYPKVESSSYAQLHNSSK 134
E P E P VPP +M P Q Y P S Y PTS + Y S +Q S
Sbjct: 144 ESPPESPPVPPGSYMPPSQSYMPPPQPPPSYY----PPTSSQPYLPPAQPSPSQSPPSQS 199
Query: 135 Y 135
Y
Sbjct: 200 Y 200
>HMFT_DROHY (P48590) Segmentation protein fushi tarazu
Length = 456
Score = 30.8 bits (68), Expect = 0.98
Identities = 18/43 (41%), Positives = 21/43 (47%), Gaps = 5/43 (11%)
Query: 91 PPQYYNKDPSSKSSSYYSAEDPTSPKEYPKVESSSYAQLHNSS 133
PP YY D +S SSSYY + P Y + SSYA S
Sbjct: 22 PPTYY--DNTSSSSSYYQSSQGWQPASY---QGSSYAHYSQES 59
>APXL_HUMAN (Q13796) Apical-like protein (APXL protein)
Length = 1616
Score = 30.8 bits (68), Expect = 0.98
Identities = 22/80 (27%), Positives = 36/80 (44%), Gaps = 12/80 (15%)
Query: 34 PKDPKHNNQGVRHEYCDQQAVIHSTEGTIRVSPDRKHFA-LYYPEDPKEDPSVPPWMSPP 92
P+ P + +G R EY + + +P R +F ++Y D K+ PS PP PP
Sbjct: 270 PRVPGDSGKGPRPEYNAEPKL---------AAPGRSNFGPVWYVPDKKKAPSSPP--PPP 318
Query: 93 QYYNKDPSSKSSSYYSAEDP 112
D + + S+ A+ P
Sbjct: 319 PPLRSDSFAATKSHEKAQGP 338
>TOF2_YEAST (Q02208) Topoisomerase 1-associated factor 2
Length = 771
Score = 30.4 bits (67), Expect = 1.3
Identities = 18/63 (28%), Positives = 32/63 (50%), Gaps = 6/63 (9%)
Query: 84 SVPPWMSPPQYYNKDPSSKSSSYYSAED--PTSPKEYPKVESSSYAQ----LHNSSKYWH 137
+ P +SP YN+D + ++S + D PTSP ++S+ + L N K+ H
Sbjct: 28 NTPQSVSPMVQYNEDIITHNNSINNCSDPSPTSPSSQNSIQSNRSSDFINYLPNCKKFLH 87
Query: 138 YSD 140
++D
Sbjct: 88 FTD 90
>SMA1_COTJA (Q9I962) Mothers against decapentaplegic homolog 1 (SMAD
1) (Mothers against DPP homolog 1) (Mad-related protein
1)
Length = 465
Score = 30.4 bits (67), Expect = 1.3
Identities = 28/95 (29%), Positives = 42/95 (43%), Gaps = 12/95 (12%)
Query: 30 SPSLPKD--PKHNNQGVRHEYCDQQAVIHSTEGTIRVSPDRKHFALYYPEDPKEDPSVPP 87
SP LP P+H+ EY Q +++ + P H A + P+ ++ S P
Sbjct: 132 SPVLPPVLVPRHS------EYNPQHSLLAQFRNLGQNEPHMPHNATF-PDSFQQPNSHPF 184
Query: 88 WMSPPQYYNKDPSSKSSSY---YSAEDPTSPKEYP 119
SP Y P S SS+Y ++ DP SP + P
Sbjct: 185 PHSPNSSYPNSPGSSSSTYPHSPASSDPGSPFQMP 219
>RPB1_CAEEL (P16356) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6)
Length = 1852
Score = 30.4 bits (67), Expect = 1.3
Identities = 19/53 (35%), Positives = 21/53 (38%), Gaps = 1/53 (1%)
Query: 74 YYPEDPKEDPSVPPWM-SPPQYYNKDPSSKSSSYYSAEDPTSPKEYPKVESSS 125
Y P P+ PS P + S P Y P SS YS PT P SS
Sbjct: 1744 YSPTSPQYSPSSPTYTPSSPTYNPTSPRGFSSPQYSPTSPTYSPTSPSYTPSS 1796
Score = 30.4 bits (67), Expect = 1.3
Identities = 20/57 (35%), Positives = 24/57 (42%), Gaps = 11/57 (19%)
Query: 74 YYPEDPKEDPSVPPWMSPPQYYNKDPS--------SKSSSYYSAEDPT---SPKEYP 119
Y P P +P+ P S PQY P+ + SS YS PT SP E P
Sbjct: 1758 YTPSSPTYNPTSPRGFSSPQYSPTSPTYSPTSPSYTPSSPQYSPTSPTYTPSPSEQP 1814
Score = 28.9 bits (63), Expect = 3.7
Identities = 25/69 (36%), Positives = 28/69 (40%), Gaps = 9/69 (13%)
Query: 65 SPDRKHFALYYPEDPKEDPSVP------PWMSP--PQYYNKDPS-SKSSSYYSAEDPTSP 115
SP + Y P PK PS P P SP PQY P S SS Y+ PT
Sbjct: 1707 SPSYESGGGYSPSSPKYSPSSPTYSPTSPSYSPTSPQYSPTSPQYSPSSPTYTPSSPTYN 1766
Query: 116 KEYPKVESS 124
P+ SS
Sbjct: 1767 PTSPRGFSS 1775
>KGP3_DROME (P32023) cGMP-dependent protein kinase, isozyme 2 forms
cD5/T2 (EC 2.7.1.37) (CGK) (Foraging protein)
Length = 934
Score = 30.4 bits (67), Expect = 1.3
Identities = 24/99 (24%), Positives = 39/99 (39%), Gaps = 9/99 (9%)
Query: 41 NQGVRHEYCDQQAVIHSTEGTIRVSPDRKHFALYYPEDPKEDPSVPP-----WMSPPQYY 95
+Q HE + + +I+ P H+ L DP E P VP + PP
Sbjct: 120 DQDQEHEELEDASEGKEKLASIQEPPAVNHYVL----DPTERPRVPRPRQQFSVKPPSLR 175
Query: 96 NKDPSSKSSSYYSAEDPTSPKEYPKVESSSYAQLHNSSK 134
S+ SY + P KE SS+Y+ ++++
Sbjct: 176 RSQTMSQPPSYATLRSPPKIKENLSKSSSAYSTFSSAAE 214
>ATXL_MOUSE (Q7TQH0) Ataxin-2-like protein
Length = 1049
Score = 30.4 bits (67), Expect = 1.3
Identities = 32/134 (23%), Positives = 50/134 (36%), Gaps = 21/134 (15%)
Query: 21 GKGVKNDDASPSLPKDPKHNNQGVRHEYCDQQAVIHSTEGTIRVSPDRKHFALYYPEDPK 80
G+ K A SLP +Q Q A + + +P ++ Y P +P+
Sbjct: 742 GQQGKYRGAKGSLPPQRSDQHQPASAPPMMQAAAAAAGPPLVAATP----YSSYIPYNPQ 797
Query: 81 EDPSVPPWMSPPQYYNKDP-----------SSKSSSYYSAEDPTSPKEYPKVESSS---- 125
+ P P M P +Y P S S+ A +S +YP E +
Sbjct: 798 QFPGQPAMMQPMAHYPSQPVFAPMLQSNPRMLTSGSHPQAIVSSSTPQYPAAEQPTPQAL 857
Query: 126 YAQLHNSSKYWHYS 139
YA +H S Y H++
Sbjct: 858 YATVHQS--YPHHA 869
>RI15_YEAST (P43565) Serine/threonine-protein kinase RIM15 (EC
2.7.1.37)
Length = 1770
Score = 30.0 bits (66), Expect = 1.7
Identities = 14/55 (25%), Positives = 27/55 (48%), Gaps = 3/55 (5%)
Query: 57 STEGTIRVSPDRKHFALYYPEDPKEDP---SVPPWMSPPQYYNKDPSSKSSSYYS 108
++ I +SP AL+YP+D K++ P +++P K +K ++S
Sbjct: 1119 NSNNNISLSPAPSDLALFYPDDSKQNKKFFGTPDYLAPETIEGKGEDNKQCDWWS 1173
>PAD3_RAT (Q9Z340) Partitioning-defective 3 homolog (PARD-3) (PAR-3)
(Atypical PKC isotype-specific interacting protein)
(ASIP) (Atypical PKC specific binding protein) (ASBP)
Length = 1337
Score = 30.0 bits (66), Expect = 1.7
Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Query: 79 PKEDPSVPPWMSPPQYYNKDPSSKSSSYYSAEDPTSPK-EYPKVESSSYAQLHNSSK 134
P+ P P PP+ + P S++ S + PT+P P V S+S ++N+ +
Sbjct: 399 PQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPTAPALAPPNVLSTSVGSVYNTKR 455
>NFC2_HUMAN (Q13469) Nuclear factor of activated T-cells,
cytoplasmic 2 (T cell transcription factor NFAT1) (NFAT
pre-existing subunit) (NF-ATp)
Length = 925
Score = 30.0 bits (66), Expect = 1.7
Identities = 15/42 (35%), Positives = 25/42 (58%), Gaps = 2/42 (4%)
Query: 84 SVPPWMSPPQYYNKDPSSKSSSYYSAEDPTSPKEYPKVESSS 125
++PP SP + ++ PS + SS+ + +D SP YP V S+
Sbjct: 262 ALPPGASPQR--SRSPSPQPSSHVAPQDHGSPAGYPPVAGSA 301
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.307 0.127 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,912,889
Number of Sequences: 164201
Number of extensions: 871943
Number of successful extensions: 2288
Number of sequences better than 10.0: 80
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 63
Number of HSP's that attempted gapping in prelim test: 2216
Number of HSP's gapped (non-prelim): 142
length of query: 141
length of database: 59,974,054
effective HSP length: 99
effective length of query: 42
effective length of database: 43,718,155
effective search space: 1836162510
effective search space used: 1836162510
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0074.7