BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0074.12
(369 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MR11_METMA (Q8PUY5) DNA double-strand break repair protein mre11 36 0.14
ALA1_CANAL (O13368) Agglutinin-like protein ALA1 precursor (Aggl... 34 0.52
AMYH_YEAST (P08640) Glucoamylase S1/S2 precursor (EC 3.2.1.3) (G... 34 0.67
TCG1_MOUSE (Q8CGF7) Transcription elongation regulator 1 (TATA b... 33 1.2
LDH_OCEIH (Q8ELF0) L-lactate dehydrogenase (EC 1.1.1.27) (L-LDH) 33 1.2
RPB1_ENCCU (Q8SSC4) DNA-directed RNA polymerase II largest subun... 32 2.0
LEU1_BUCTS (O31287) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 32 2.0
TRI_THEAC (P96086) Tricorn protease (EC 3.4.21.-) 32 2.6
TCG1_HUMAN (O14776) Transcription elongation regulator 1 (TATA b... 32 3.3
NUC2_SCHPO (P10505) Nuclear scaffold-like protein p76 31 4.4
FIT1_YEAST (Q04433) Facilitator of iron transport 1 precursor 31 4.4
DAN4_YEAST (P47179) Cell wall protein DAN4 precursor 31 4.4
Y111_ENCCU (Q8STB5) Hypothetical protein ECU01_0110/ECU01_1500/E... 31 5.7
PP3A_MOUSE (Q99MR9) Protein phosphatase 1, regulatory subunit 3A... 31 5.7
CAB2_AQUAE (O67233) Carbamoyl-phosphate synthase large chain, C-... 31 5.7
YQ53_CAEEL (Q09249) Hypothetical protein C16C10.3 in chromosome III 30 7.5
PRGR_RAT (Q63449) Progesterone receptor (PR) 30 7.5
HMDL_DROME (P20009) Homeotic distal-less protein (Protein brista) 30 7.5
T3C3_HUMAN (Q9Y5Q9) General transcription factor 3C polypeptide ... 30 9.7
SYM_METMA (Q8PYJ4) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 30 9.7
>MR11_METMA (Q8PUY5) DNA double-strand break repair protein mre11
Length = 617
Score = 36.2 bits (82), Expect = 0.14
Identities = 35/120 (29%), Positives = 51/120 (42%), Gaps = 10/120 (8%)
Query: 145 RSSEQVSPHRVVFSETTLEDGAQLPFEASSDVHLLPEVVSRVELGSAETEIESHESSTSE 204
+S + P R+ SE+ L + FE V PE + GS ET +E S +
Sbjct: 465 KSETLMPPVRIQRSES-LNESLNKIFEKHDVVPESPETAGSIA-GSVETAVEDEISKVTP 522
Query: 205 DSSLITTPVDSTPYA------TRSDAITP--DVNKTFVLKTTDLVLRNPRQVFGNLLEGK 256
DS +IT P S+P + T AI+P + +LK + + V G L E K
Sbjct: 523 DSGVITAPQSSSPVSFSDNSQTGFSAISPPESIPSPEILKENSEADADEKPVDGKLSEEK 582
>ALA1_CANAL (O13368) Agglutinin-like protein ALA1 precursor
(Agglutinin-like adhesin)
Length = 1419
Score = 34.3 bits (77), Expect = 0.52
Identities = 26/108 (24%), Positives = 45/108 (41%), Gaps = 2/108 (1%)
Query: 135 FEKSSLVEIQRSSEQVSPHRVVFSETTLEDGAQLP--FEASSDVHLLPEVVSRVELGSAE 192
F +LV SS S + +T+ P SD + + S VE+ S
Sbjct: 797 FATDALVSSDASSILSSDTSSYYPSSTISSSDDFPHTIAGESDSLSISFITSTVEISSDS 856
Query: 193 TEIESHESSTSEDSSLITTPVDSTPYATRSDAITPDVNKTFVLKTTDL 240
+ S +S+ + SS + + S+P + +SD +T T V+ + L
Sbjct: 857 VSLTSDPASSFDSSSSLNSDSSSSPSSDQSDILTSSSFSTLVVPSFSL 904
>AMYH_YEAST (P08640) Glucoamylase S1/S2 precursor (EC 3.2.1.3)
(Glucan 1,4-alpha-glucosidase) (1,4-alpha-D-glucan
glucohydrolase)
Length = 1367
Score = 33.9 bits (76), Expect = 0.67
Identities = 27/88 (30%), Positives = 39/88 (43%), Gaps = 1/88 (1%)
Query: 146 SSEQVSPHRVVFSETTLEDGAQLPFEASSDVHLLPEVVSRVELGSAETEIESHESSTSED 205
SS S V S TT A +P +SS V+ S+ + + SST+E
Sbjct: 532 SSTTESSSAPVTSSTTESSSAPVPTPSSSTTESSSTPVTSSTTESSSAPVPTPSSSTTES 591
Query: 206 SSL-ITTPVDSTPYATRSDAITPDVNKT 232
SS + TP ST ++ + A TP + T
Sbjct: 592 SSAPVPTPSSSTTESSSAPAPTPSSSTT 619
Score = 30.4 bits (67), Expect = 7.5
Identities = 22/76 (28%), Positives = 34/76 (43%), Gaps = 1/76 (1%)
Query: 158 SETTLEDGAQLPFEASSDVHLLPEVVSRVELGSAETEIESHESSTSEDSSL-ITTPVDST 216
S TT A +P +SS V+ S+ + + SST+E SS + TP ST
Sbjct: 727 SSTTESSSAPVPTPSSSTTESSSAPVTSSTTESSSAPVPTPSSSTTESSSAPVPTPSSST 786
Query: 217 PYATRSDAITPDVNKT 232
++ + TP + T
Sbjct: 787 TESSSAPVPTPSSSTT 802
>TCG1_MOUSE (Q8CGF7) Transcription elongation regulator 1 (TATA
box-binding protein-associated factor 2S) (Transcription
factor CA150) (p144) (Formin-binding protein 28) (FBP
28)
Length = 1100
Score = 33.1 bits (74), Expect = 1.2
Identities = 43/187 (22%), Positives = 71/187 (36%), Gaps = 21/187 (11%)
Query: 104 IHSWEEFKVAFRLYFVYNRPNGFSTTTVKYGFEK-SSLVEIQRSSEQVSPHRVVFSETTL 162
+ W E+K A + YN T++ +EK L E ++ E++ SE L
Sbjct: 434 VSEWTEYKTADGKTYYYNN------RTLESTWEKPQELKEKEKLDEKIKEPIKEASEEPL 487
Query: 163 EDGAQLPFEASSDVHLLPEVVSRVELGSAETEIESHESSTSEDSSLITTPVDSTPYATRS 222
P E + E V ++ E E+ E + + + TTP+ TP+
Sbjct: 488 ------PMETEEE-DPKEEPVKEIKEEPKEEEMTEEEKAAQKAKPVATTPIPGTPWCV-- 538
Query: 223 DAITPDVNKTFVLKTTDLVL-RNPRQVFGNLLEGKFVQKNNEAQFEKHFDKLLMQFHNSS 281
T D F TT L + P + G K +Q E +K + + H +
Sbjct: 539 -VWTGDERVFFYNPTTRLSMWDRPDDLIGRADVDKIIQ---EPPHKKGLEDMKKLRHPAP 594
Query: 282 TTVDVQK 288
T + +QK
Sbjct: 595 TMLSIQK 601
>LDH_OCEIH (Q8ELF0) L-lactate dehydrogenase (EC 1.1.1.27) (L-LDH)
Length = 321
Score = 33.1 bits (74), Expect = 1.2
Identities = 25/78 (32%), Positives = 39/78 (49%), Gaps = 9/78 (11%)
Query: 152 PHRVVFSETTLEDGAQLPFEASSDVHLLPEVVSRVELGS-AETEIESHESSTSEDSSLIT 210
PHRV+ S TTL D A+L +E HL P+ + +G +TE+ ++T L+T
Sbjct: 143 PHRVIGSGTTL-DTARLRYELGEYFHLSPKNIHAYIIGEHGDTELPLWSTATIGTVPLLT 201
Query: 211 TPVDSTPYATRSDAITPD 228
Y RS++ T +
Sbjct: 202 -------YLNRSESYTTE 212
>RPB1_ENCCU (Q8SSC4) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) (RNA polymerase II subunit 1)
Length = 1599
Score = 32.3 bits (72), Expect = 2.0
Identities = 21/50 (42%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
Query: 13 SAQRDPRSIFYSP--PSQPNTSPYYCHNVPSYYPQTHS-SLQLSEFNGEN 59
S P S YSP PS TSP Y PSY P + S S+ +S F+ +N
Sbjct: 1533 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSVSMSSFSNKN 1582
>LEU1_BUCTS (O31287) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
(Fragment)
Length = 375
Score = 32.3 bits (72), Expect = 2.0
Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 7/81 (8%)
Query: 228 DVNKTFV--LKTTDLVLRNPRQVFGNLLEGKFVQKNNEAQFEKHFDKLLMQFHNSSTTVD 285
D+N+ +V LK D QVF LE N E + ++HF H+ ST V
Sbjct: 209 DLNQLYVNFLKLAD----KKGQVFDYDLEALAFISNQEDKDQEHFQLKYFSIHSGSTGVS 264
Query: 286 VQKIGVPC-PKVFEKMLVTRN 305
+ + C K+ +++ TRN
Sbjct: 265 TASVKLLCGNKILNEIITTRN 285
>TRI_THEAC (P96086) Tricorn protease (EC 3.4.21.-)
Length = 1071
Score = 32.0 bits (71), Expect = 2.6
Identities = 31/115 (26%), Positives = 52/115 (44%), Gaps = 11/115 (9%)
Query: 164 DGAQLPFEASSDVHLL---PEVVSRVELG---SAETEIESHESSTSEDSSLITTPVDSTP 217
DG ++ F +++ E + ++E+G S E I S S +ED S + D
Sbjct: 281 DGRRILFSKGGSIYIFNPDTEKIEKIEIGDLESPEDRIISIPSKFAEDFSPLDG--DLIA 338
Query: 218 YATRSDAITPDVNKTFVLKTTD-LVLRNPRQVFGNLLEGKFVQKNNEAQFEKHFD 271
+ +R A DV+ T+VLK + L +R R+ G + F+ E F +D
Sbjct: 339 FVSRGQAFIQDVSGTYVLKVPEPLRIRYVRR--GGDTKVAFIHGTREGDFLGIYD 391
>TCG1_HUMAN (O14776) Transcription elongation regulator 1 (TATA
box-binding protein-associated factor 2S) (Transcription
factor CA150)
Length = 1098
Score = 31.6 bits (70), Expect = 3.3
Identities = 42/192 (21%), Positives = 69/192 (35%), Gaps = 31/192 (16%)
Query: 104 IHSWEEFKVAFRLYFVYNRPNGFSTTTVKYGFEKSSLVE------IQRSSEQVSPHRVVF 157
+ W E+K A + YN ST ++ +E I+ SE+ P
Sbjct: 432 VSEWTEYKTADGKTYYYNNRTLESTWEKPQELKEKEKLEEKIKEPIKEPSEEPLP----- 486
Query: 158 SETTLEDGAQLPFEASSDVHLLPEVVSRVELGSAETEIESHESSTSEDSSLITTPVDSTP 217
ET ED + P + ++ E E+ E + + + T P+ TP
Sbjct: 487 METEEEDPKEEP-------------IKEIKEEPKEEEMTEEEKAAQKAKPVATAPIPGTP 533
Query: 218 YATRSDAITPDVNKTFVLKTTDLVL-RNPRQVFGNLLEGKFVQKNNEAQFEKHFDKLLMQ 276
+ T D F TT L + P + G K +Q E +K ++L
Sbjct: 534 WCV---VWTGDERVFFYNPTTRLSMWDRPDDLIGRADVDKIIQ---EPPHKKGMEELKKL 587
Query: 277 FHNSSTTVDVQK 288
H + T + +QK
Sbjct: 588 RHPTPTMLSIQK 599
>NUC2_SCHPO (P10505) Nuclear scaffold-like protein p76
Length = 665
Score = 31.2 bits (69), Expect = 4.4
Identities = 30/121 (24%), Positives = 50/121 (40%), Gaps = 9/121 (7%)
Query: 44 PQTHSSLQLSEFNGENPEYWLFMADCYFLQNPYPWETRFQLLAIHLGGK---ALTWFRLS 100
P ++ + + E N +PE W +A+C+ LQ + + AI L A T
Sbjct: 415 PLSYLAHETLETNPYSPESWCILANCFSLQREHSQALKCINRAIQLDPTFEYAYTLQGHE 474
Query: 101 HQQIHSWEEFKVAFRLYF-----VYNRPNGFSTTTVKYGFEKSSLVEIQRSSEQVSPHRV 155
H +E+ K +FR YN G +K G + QR++E ++P+
Sbjct: 475 HSANEEYEKSKTSFRKAIRVNVRHYNAWYGLGMVYLKTGRNDQADFHFQRAAE-INPNNS 533
Query: 156 V 156
V
Sbjct: 534 V 534
>FIT1_YEAST (Q04433) Facilitator of iron transport 1 precursor
Length = 528
Score = 31.2 bits (69), Expect = 4.4
Identities = 35/107 (32%), Positives = 48/107 (44%), Gaps = 8/107 (7%)
Query: 136 EKSSLVEIQRSSEQVSPHRVVFSETTLEDGAQLPFEASSDVHLLPEV-VSRVELGSAETE 194
E SS VE + E S +ET+ E SS V + V S VE S+ +
Sbjct: 310 ETSSAVETSSAVETSSA-----AETSSAAETSSAVETSSAVEISSAVETSAVETSSSSST 364
Query: 195 IE--SHESSTSEDSSLITTPVDSTPYATRSDAITPDVNKTFVLKTTD 239
IE S +S + +SL ++ S+P T S A T V TF TT+
Sbjct: 365 IETTSVKSLSPTQTSLSSSVQASSPIETSSAAKTSSVVPTFSSTTTE 411
>DAN4_YEAST (P47179) Cell wall protein DAN4 precursor
Length = 1161
Score = 31.2 bits (69), Expect = 4.4
Identities = 19/68 (27%), Positives = 28/68 (40%)
Query: 178 LLPEVVSRVELGSAETEIESHESSTSEDSSLITTPVDSTPYATRSDAITPDVNKTFVLKT 237
L P + S + T I + S+T+ SS TTP + T + + TP + T T
Sbjct: 104 LKPAISSALSKDGIYTAIPTSTSTTTTKSSTSTTPTTTITSTTSTTSTTPTTSTTSTTPT 163
Query: 238 TDLVLRNP 245
T P
Sbjct: 164 TSTTSTTP 171
>Y111_ENCCU (Q8STB5) Hypothetical protein
ECU01_0110/ECU01_1500/ECU08_0040
Length = 592
Score = 30.8 bits (68), Expect = 5.7
Identities = 19/70 (27%), Positives = 40/70 (57%), Gaps = 2/70 (2%)
Query: 232 TFVLKTTDLVLRNPRQVFGNLLEGKFVQKNNEAQFEKHFDKLLMQFHNSSTTVDVQKIGV 291
T +K + +L + V +L+ G KN++ +F+K FDK++ ++ N + +++ +I +
Sbjct: 64 TRYMKDPEFLLYSLSSVVWDLVVGHVSYKNDD-RFDKLFDKMMSKYLN-NVSINASRIYI 121
Query: 292 PCPKVFEKML 301
K F +ML
Sbjct: 122 RGNKTFSEML 131
>PP3A_MOUSE (Q99MR9) Protein phosphatase 1, regulatory subunit 3A
(Protein phosphatase 1 glycogen-associated regulatory
subunit) (Protein phosphatase type-1 glycogen targeting
subunit) (RGL)
Length = 1089
Score = 30.8 bits (68), Expect = 5.7
Identities = 21/76 (27%), Positives = 38/76 (49%), Gaps = 6/76 (7%)
Query: 139 SLVEIQRSSEQVSPHRVVFSETTLEDGAQLP-----FEASSDVHLLPEVVSRVELGSAET 193
S+ I S+ ++ ++ S+ L G LP F+A++D+ LL E+ R + + +
Sbjct: 818 SVTSIDEPSQVITGNQKATSKLDLHLGV-LPTDRAIFQANADLELLQELSRRTDFNAVPS 876
Query: 194 EIESHESSTSEDSSLI 209
S +S S DSS +
Sbjct: 877 AFNSDTASASRDSSQV 892
>CAB2_AQUAE (O67233) Carbamoyl-phosphate synthase large chain,
C-terminal section (EC 6.3.5.5) (Carbamoyl-phosphate
synthetase ammonia chain)
Length = 537
Score = 30.8 bits (68), Expect = 5.7
Identities = 44/187 (23%), Positives = 74/187 (39%), Gaps = 34/187 (18%)
Query: 135 FEKSSLVEIQRSSEQVSPHRVVFSETTLEDGAQLPFEASSDVH--LLPEVVSRVELGSAE 192
+++ L E + VS R V + L++ +L +A SD L+ V+ +E E
Sbjct: 172 YDEEELKEYLEEAVSVSHERPVLIDKFLDNSIELDVDAVSDGKDVLIGAVMEHIE----E 227
Query: 193 TEIESHESSTS-EDSSLITTPVDSTPYATRSDAITPDVNKTFVLKTTDLVLRNPRQVFGN 251
+ S +S+TS SL V+ TR A+ +V
Sbjct: 228 AGVHSGDSATSIPPYSLSKEIVEEVKEQTRKLAVALEVK--------------------G 267
Query: 252 LLEGKFVQKNNEAQFEKHFDKLLMQFHNSSTTVDVQK-IGVPCPKVFEKMLVTRNTRICV 310
L+ ++ +NNE L + S T V K IG P K+ K+ + ++ R +
Sbjct: 268 LINVQYAVQNNEVYV------LEVNPRASRTVPFVSKSIGYPLAKIATKVAIGKSLREIL 321
Query: 311 DNTFEKL 317
FE+L
Sbjct: 322 PEVFERL 328
>YQ53_CAEEL (Q09249) Hypothetical protein C16C10.3 in chromosome III
Length = 1032
Score = 30.4 bits (67), Expect = 7.5
Identities = 33/136 (24%), Positives = 57/136 (41%), Gaps = 12/136 (8%)
Query: 108 EEFKVAFRL-------YFVYNRPNGFSTTTVKYGFEKSSLVEIQRSSEQVSPHRVVFSET 160
EE+K +L Y V +P GF T + E+++ +E+ R E V+ + + +E
Sbjct: 173 EEWKFVSKLSRRKDDSYLVVLKPAGFVYTQGEVAQEEANRMELTRIIEIVTSQK-LNNED 231
Query: 161 TLEDGAQLPFEASSDVHLLPEVVSRVELGSAETEIESHESSTSEDSSLITTPVDSTPYAT 220
L+ G F S H P+ +S + G A+ S S + +T +P+
Sbjct: 232 YLQFG-NATFPRLSPPHSEPDAISEIRSGFAKV---SRLSQNGGGKAFMTVDTKISPFYK 287
Query: 221 RSDAITPDVNKTFVLK 236
+ I NK +K
Sbjct: 288 DTSVIKFSSNKLSEMK 303
>PRGR_RAT (Q63449) Progesterone receptor (PR)
Length = 923
Score = 30.4 bits (67), Expect = 7.5
Identities = 16/81 (19%), Positives = 41/81 (49%), Gaps = 1/81 (1%)
Query: 135 FEKSSLVEIQRSSEQVS-PHRVVFSETTLEDGAQLPFEASSDVHLLPEVVSRVELGSAET 193
F+K + V + R+ + V+ P V F + G ++ F + ++ L+P +++ + +
Sbjct: 629 FKKFNKVRVMRALDGVALPQSVAFPNESQTLGQRITFSPNQEIQLVPPLINLLMSIEPDV 688
Query: 194 EIESHESSTSEDSSLITTPVD 214
H+++ + SS + T ++
Sbjct: 689 VYAGHDNTKPDTSSSLLTSLN 709
>HMDL_DROME (P20009) Homeotic distal-less protein (Protein brista)
Length = 327
Score = 30.4 bits (67), Expect = 7.5
Identities = 15/46 (32%), Positives = 21/46 (45%), Gaps = 2/46 (4%)
Query: 18 PRSIFYSPPSQPNTSPYYCHNVPSYYPQTHSSLQLSEFNGENPEYW 63
P I + PP P +P H + +PQTH + G P+YW
Sbjct: 268 PHWIDHKPP--PQMTPQPPHPAATLHPQTHHHNPPPQMGGYVPQYW 311
>T3C3_HUMAN (Q9Y5Q9) General transcription factor 3C polypeptide 3
(Transcription factor IIIC-gamma subunit) (TF3C-gamma)
(TFIIIC 102 kDa subunit) (TFIIIC102)
Length = 886
Score = 30.0 bits (66), Expect = 9.7
Identities = 20/63 (31%), Positives = 31/63 (48%), Gaps = 5/63 (7%)
Query: 81 RFQLLAIHLG-GKALTWFRLSHQQIHSWEEFKVAFRLYFVYNRPNGFSTTTVKYGFEKSS 139
+F+L+A HL W RL+ + + K A F Y + + T V+Y +E+SS
Sbjct: 204 QFELIAAHLNPSDTEEWVRLAEMSLEQ-DNIKQAI---FCYTKALKYEPTNVRYLWERSS 259
Query: 140 LVE 142
L E
Sbjct: 260 LYE 262
>SYM_METMA (Q8PYJ4) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 715
Score = 30.0 bits (66), Expect = 9.7
Identities = 13/40 (32%), Positives = 23/40 (57%)
Query: 156 VFSETTLEDGAQLPFEASSDVHLLPEVVSRVELGSAETEI 195
+++ + ++G ++ F SD H P VV+ ELG TE+
Sbjct: 39 IYARSLKKEGREVTFVCGSDTHGTPIVVNAEELGITPTEL 78
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,138,006
Number of Sequences: 164201
Number of extensions: 1694218
Number of successful extensions: 5168
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 5126
Number of HSP's gapped (non-prelim): 51
length of query: 369
length of database: 59,974,054
effective HSP length: 112
effective length of query: 257
effective length of database: 41,583,542
effective search space: 10686970294
effective search space used: 10686970294
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0074.12