BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0065.1
         (378 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

SP20_HUMAN (Q8N0X7) Spartin (Trans-activated by hepatitis C viru...    47  1e-04
SP20_MOUSE (Q8R1X6) Spartin                                            44  9e-04
TOP2_CRIFA (P27570) DNA topoisomerase II (EC 5.99.1.3)                 34  0.53
RPC1_SCHPO (O94666) DNA-directed RNA polymerase III largest subu...    34  0.70
VACA_HELPY (P55981) Vacuolating cytotoxin precursor                    33  0.91
MUTA_HUMAN (P22033) Methylmalonyl-CoA mutase, mitochondrial prec...    33  1.6
Y413_RICPR (Q9ZDB9) Hypothetical protein RP413                         32  2.0
VT1B_HUMAN (Q9UEU0) Vesicle transport through interaction with t...    31  4.5
VACA_HELPJ (Q9ZKW5) Vacuolating cytotoxin precursor                    31  4.5
DHAF_VIBHA (Q56694) NADP-dependent fatty aldehyde dehydrogenase ...    31  5.9
YJJA_ECOLI (P18390) Hypothetical protein yjjA precursor (Protein...    30  7.7
AMID_PSECL (P27765) Amidase (EC 3.5.1.4)                               30  7.7

>SP20_HUMAN (Q8N0X7) Spartin (Trans-activated by hepatitis C virus
           core protein 1)
          Length = 666

 Score = 46.6 bits (109), Expect = 1e-04
 Identities = 36/136 (26%), Positives = 63/136 (45%), Gaps = 15/136 (11%)

Query: 231 AMNENLKRVRQLTTMTEKLSKSLLDGVGIMS---GSVMAPVLKSQPGQAFLKMLPGE--- 284
           A+ + L   +Q T    K+S+ L+DGV  ++   G  +AP +K    +   + L  +   
Sbjct: 472 AVTKGLYIAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDKDG 531

Query: 285 ---------VLLASLDAVNKVFEATEAAQKQSLSATSQAATRMVSNRYGEEAGEATEHVF 335
                    V  +S+   + V++  E A K  ++  S    + V  +YG  AGEAT H  
Sbjct: 532 KSPLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGYNAGEATHHAV 591

Query: 336 ATAGHAANTAWNVSKI 351
            +A +   TA+N++ I
Sbjct: 592 DSAVNVGVTAYNINNI 607


>SP20_MOUSE (Q8R1X6) Spartin
          Length = 671

 Score = 43.5 bits (101), Expect = 9e-04
 Identities = 35/136 (25%), Positives = 61/136 (44%), Gaps = 15/136 (11%)

Query: 231 AMNENLKRVRQLTTMTEKLSKSLLDGVGIMS---GSVMAPVLKSQPGQAFLKMLPGE--- 284
           A+   L   +Q T    K+S+ L+DGV  ++   G  +AP +K    +   + L  +   
Sbjct: 476 AVTRGLYIAKQATGGAAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDG 535

Query: 285 ---------VLLASLDAVNKVFEATEAAQKQSLSATSQAATRMVSNRYGEEAGEATEHVF 335
                    V  +S+   + V++  E A K  ++  S    + V  +YG  AGEAT +  
Sbjct: 536 KSALDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAV 595

Query: 336 ATAGHAANTAWNVSKI 351
            +A +   TA+N+  I
Sbjct: 596 DSAINVGLTAYNIDNI 611


>TOP2_CRIFA (P27570) DNA topoisomerase II (EC 5.99.1.3)
          Length = 1239

 Score = 34.3 bits (77), Expect = 0.53
 Identities = 37/122 (30%), Positives = 52/122 (42%), Gaps = 11/122 (9%)

Query: 264  VMAPVLKSQPGQAFLKML-PGEVLLASLDAVNKVFEATEAAQKQSLSATSQAATRMVSNR 322
            + A + K+Q   A LK   P ++ L  LD  +K F+  E     S+    Q   R  S  
Sbjct: 1074 LQADIKKTQDSIAVLKQTTPVKMWLTDLDKFDKTFQEYERVLIHSI----QKEQRPASIT 1129

Query: 323  YGEEAGEATEHVF---ATAGHAANTAWNVSKIRKAINPVT---PASTAGGALRNSIKNRN 376
             GEE     +      A A  AA++++ V   R    P +   P  T GGAL +    RN
Sbjct: 1130 GGEEVPALRQPPLMLEAPAKGAASSSYRVHICRYEEPPASKRKPEDTYGGALSSGGSTRN 1189

Query: 377  VG 378
            VG
Sbjct: 1190 VG 1191


>RPC1_SCHPO (O94666) DNA-directed RNA polymerase III largest subunit
            (EC 2.7.7.6)
          Length = 1405

 Score = 33.9 bits (76), Expect = 0.70
 Identities = 33/128 (25%), Positives = 55/128 (42%), Gaps = 8/128 (6%)

Query: 236  LKRVRQLTTMTEKLSKSLLDGVGIMSGSVMAPVLKSQPGQAFLKMLPGEVLLASLDAVNK 295
            L  VR+   +   L +  +D +  M G   APV K +  +  +++   E  L S   V++
Sbjct: 976  LASVRERRDLAPMLEEPDMDDLDDMEGDEFAPVAKRKSVENIIRVT--EKQLRSF--VDR 1031

Query: 296  VFEATEAAQKQSLSATSQAATRMVSNRYGEEAGEATEHVFATAGHAANTAWNVSKIRKAI 355
             +E    A+ +  +A      + +    GE   + T   F  AG AA T   V +I++ I
Sbjct: 1032 CWEKYMRAKVEPGTAVGAIGAQSI----GEPGTQMTLKTFHFAGVAAQTTLGVPRIKEII 1087

Query: 356  NPVTPAST 363
            N     ST
Sbjct: 1088 NAAKTIST 1095


>VACA_HELPY (P55981) Vacuolating cytotoxin precursor
          Length = 1290

 Score = 33.5 bits (75), Expect = 0.91
 Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 1/65 (1%)

Query: 202 QTILTDNKNNGLVRQSVSNNKTADATKK-NAMNENLKRVRQLTTMTEKLSKSLLDGVGIM 260
           QT+L D+ + G  RQ + N  T + TK+ NA    L  +  L   T  L    L    I+
Sbjct: 913 QTLLIDSHDAGYARQMIDNTSTGEITKQLNAATTTLNNIASLEHKTSSLQTLSLSNAMIL 972

Query: 261 SGSVM 265
           +  ++
Sbjct: 973 NSRLV 977


>MUTA_HUMAN (P22033) Methylmalonyl-CoA mutase, mitochondrial
           precursor (EC 5.4.99.2) (MCM)
          Length = 750

 Score = 32.7 bits (73), Expect = 1.6
 Identities = 38/164 (23%), Positives = 69/164 (41%), Gaps = 24/164 (14%)

Query: 171 LAKAIAGGTGQIVKGIFMCSNAYTNQVQSGGQTILTDNKNNGLVRQSVSNNKTADATKKN 230
           +AKA+A G  ++   I  C+     ++ SG + I+  NK       +V      + + +N
Sbjct: 455 MAKAVAEGIPKL--RIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRN 512

Query: 231 AMNENLKRVRQLT--TMTEKLSKSLLDGVGIMSGSVMAPVLKSQPGQAFLKMLPGEVLLA 288
              E LK+++      + E    +L +      G+++A  + +   +  +    GE+   
Sbjct: 513 RQIEKLKKIKSSRDQALAEHCLAALTECAASGDGNILALAVDASRARCTV----GEIT-- 566

Query: 289 SLDAVNKVFEATEAAQKQSLSATSQAATRMVSNRYGEEAGEATE 332
             DA+ KVF               +A  RMVS  Y +E GE+ E
Sbjct: 567 --DALKKVF------------GEHKANDRMVSGAYRQEFGESKE 596


>Y413_RICPR (Q9ZDB9) Hypothetical protein RP413
          Length = 219

 Score = 32.3 bits (72), Expect = 2.0
 Identities = 20/56 (35%), Positives = 28/56 (49%), Gaps = 4/56 (7%)

Query: 185 GIFM---CSNAYTNQVQSGGQTILTDNKN-NGLVRQSVSNNKTADATKKNAMNENL 236
           GIF+   C++ + N  Q      L D K   G  R+ V NNK  D  KKN + +N+
Sbjct: 11  GIFLLSSCTDNFRNYFQRSANNKLLDIKGAKGGKRKPVYNNKYIDLAKKNILEDNI 66


>VT1B_HUMAN (Q9UEU0) Vesicle transport through interaction with
           t-SNAREs homolog 1B (Vesicle transport v-SNARE protein
           Vti1-like 1) (Vti1-rp1)
          Length = 232

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 23/88 (26%), Positives = 42/88 (47%), Gaps = 6/88 (6%)

Query: 285 VLLASLDAVNKVFEATEAAQKQSLSATSQAATRMVSNRYGEEAGEATEHVFATAGHAANT 344
           +LL   +++N+   AT++ ++    AT    T  + +   EE GE  + +  T     NT
Sbjct: 131 MLLQGTESLNR---ATQSIERSHRIATE---TDQIGSEIIEELGEQRDQLERTKSRLVNT 184

Query: 345 AWNVSKIRKAINPVTPASTAGGALRNSI 372
           + N+SK RK +  ++   T    L + I
Sbjct: 185 SENLSKSRKILRSMSRKVTTNKLLLSII 212


>VACA_HELPJ (Q9ZKW5) Vacuolating cytotoxin precursor
          Length = 1288

 Score = 31.2 bits (69), Expect = 4.5
 Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 1/65 (1%)

Query: 202 QTILTDNKNNGLVRQSVSNNKTADATKK-NAMNENLKRVRQLTTMTEKLSKSLLDGVGIM 260
           QT+L D+ + G  RQ + N  T + TK+ NA  + L  V  L      L    L    I+
Sbjct: 910 QTLLIDSHDAGYARQMIDNTSTGEITKQLNAATDALNNVASLEHKQSGLQTLSLSNAMIL 969

Query: 261 SGSVM 265
           +  ++
Sbjct: 970 NSRLV 974


>DHAF_VIBHA (Q56694) NADP-dependent fatty aldehyde dehydrogenase (EC
           1.2.1.4)
          Length = 510

 Score = 30.8 bits (68), Expect = 5.9
 Identities = 26/97 (26%), Positives = 41/97 (41%), Gaps = 4/97 (4%)

Query: 284 EVLLASLDAVNKVFEATEAAQKQSLSATSQAATRMVSNRY--GEEAGEATEH--VFATAG 339
           E+L  SL A     E       Q +    + A R+V N +  G E G A  H   +  + 
Sbjct: 399 EMLAGSLTATIHATEEDYPQVSQLIPRLEEIAGRLVFNGWPTGVEVGYAMVHGGPYPAST 458

Query: 340 HAANTAWNVSKIRKAINPVTPASTAGGALRNSIKNRN 376
           H+A+T+     I + + PV   +     L +S+K  N
Sbjct: 459 HSASTSVGAEAIHRWLRPVAYQALPESLLPDSLKAEN 495


>YJJA_ECOLI (P18390) Hypothetical protein yjjA precursor (Protein
           P-18)
          Length = 164

 Score = 30.4 bits (67), Expect = 7.7
 Identities = 21/66 (31%), Positives = 34/66 (50%), Gaps = 3/66 (4%)

Query: 194 TNQVQSGGQTILTDNKNNGL-VRQSVSNNKTADAT-KKNAMNENLKRVRQLTTMTEKLSK 251
           TN + SG Q +  DN NN   + Q  +  K A  T  +N  N+ L+++  L +  +K   
Sbjct: 57  TNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLEKL-GLNSEEQKEDT 115

Query: 252 SLLDGV 257
           + LDG+
Sbjct: 116 NYLDGI 121


>AMID_PSECL (P27765) Amidase (EC 3.5.1.4)
          Length = 505

 Score = 30.4 bits (67), Expect = 7.7
 Identities = 18/58 (31%), Positives = 29/58 (49%), Gaps = 1/58 (1%)

Query: 120 GVTFPEQCYGSMGLLESFLKDHSCFSDLKTGKKGGLDWEQFAPRVEDYNHFLAKAIAG 177
           GV   E     +G +   ++D++       G  G LD  Q AP+V+DY  +L K ++G
Sbjct: 220 GVMAIEATIDHVGPITGNVRDNALMLQAMAGADG-LDPRQAAPQVDDYCSYLEKGVSG 276


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.313    0.130    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,380,701
Number of Sequences: 164201
Number of extensions: 1730104
Number of successful extensions: 3491
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 3487
Number of HSP's gapped (non-prelim): 14
length of query: 378
length of database: 59,974,054
effective HSP length: 112
effective length of query: 266
effective length of database: 41,583,542
effective search space: 11061222172
effective search space used: 11061222172
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 67 (30.4 bits)