BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0060.1
(358 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RA50_METAC (Q8TRL1) DNA double-strand break repair rad50 ATPase 36 0.13
GCC2_HUMAN (Q8IWJ2) GRIP and coiled-coil domain-containing prote... 34 0.65
B3G7_MOUSE (Q9Z222) Beta-1,3-galactosyltransferase 7 (EC 2.4.1.-... 33 1.1
REPB_AGRRH (P05683) Possible replication protein B 33 1.4
BRN_DROME (Q24157) Beta-1,3-galactosyltransferase brn (EC 2.4.1.... 33 1.4
M5_STRP5 (P02977) M protein, serotype 5 precursor 32 2.5
AROK_BUCBP (P59488) Shikimate kinase (EC 2.7.1.71) (SK) 32 2.5
LMD1_HUMAN (P29536) Leiomodin 1 (Leiomodin, muscle form) (64 kDa... 32 3.2
YEIT_ECOLI (P76440) Hypothetical oxidoreductase yeiT 31 4.2
MP54_YEAST (Q08550) Meiotic plaque component protein 54 31 4.2
DMD_HUMAN (P11532) Dystrophin 31 5.5
MLP2_YEAST (P40457) MLP2 protein (Myosin-like protein 2) 30 7.2
GSPD_ERWCA (P31701) General secretion pathway protein D precurso... 30 7.2
PTAA_ECOLI (P09323) PTS system, N-acetylglucosamine-specific IIA... 30 9.4
>RA50_METAC (Q8TRL1) DNA double-strand break repair rad50 ATPase
Length = 1074
Score = 36.2 bits (82), Expect = 0.13
Identities = 36/138 (26%), Positives = 67/138 (48%), Gaps = 20/138 (14%)
Query: 49 LVEELEKRTGQGQSAVSVDDTLKVIACREQ--QKKLSALEMELTAARKEGFVSKHLSGDN 106
L+E +R G+ ++D LK +A +E ++KL ALE+ L AA+K+ +K ++
Sbjct: 723 LIENYGERLGE------LNDKLKALAEKENLSKEKLKALELALEAAQKKENEAKKAHSES 776
Query: 107 EKQKHPTKKVLSVIGVMTTFGRRKNRDAIRKAWMPTGTAMKKLADQKGVIVRFVIGRS-- 164
EK KK+ + + M + I+ A++ LA++ G + R ++ RS
Sbjct: 777 EKLLGQAKKLQANLLSM---------ENIKHKISELEAAIRNLAEKVGFLDREILERSER 827
Query: 165 -ANRGDSLDKEIETEISQ 181
G+ L+ +E+ Q
Sbjct: 828 IRQLGEKLEGNRLSELQQ 845
>GCC2_HUMAN (Q8IWJ2) GRIP and coiled-coil domain-containing protein
2 (Golgi coiled coil protein GCC185) (CTCL tumor antigen
se1-1) (CLL-associated antigen KW-11)
Length = 1583
Score = 33.9 bits (76), Expect = 0.65
Identities = 45/189 (23%), Positives = 80/189 (41%), Gaps = 22/189 (11%)
Query: 43 AENRVHLVEELEKRTG--QGQSAVSVDDTLKVIACREQQKKLSALEMELTAARKEGFVSK 100
A R V ELEK Q +S V ++ ++ EQ+K L E+E + KE S
Sbjct: 700 AFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKVLLRKELEEIQSEKEALQSD 759
Query: 101 HLSGDNEKQKHPTKK---VLSVIGVMTTFGRRKNRDAIRKAWMPTGTAMKKLADQKG--- 154
L N +K + ++ V V T + + + K ++ +K L +QK
Sbjct: 760 LLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKEKCFIKEHENLKPLLEQKELRD 819
Query: 155 -----VIVRFVIGRSAN-RGDSLD--KEIETEISQTNDFIILDNQVEAPEERAKKIKSFF 206
++++ + +S + + D L KE+E +I L+ + + EE+ KIK
Sbjct: 820 RRAELILLKDSLAKSPSVKNDPLSSVKELEEKIEN------LEKECKEKEEKINKIKLVA 873
Query: 207 IYAVDNWDA 215
+ A D+
Sbjct: 874 VKAKKELDS 882
>B3G7_MOUSE (Q9Z222) Beta-1,3-galactosyltransferase 7 (EC 2.4.1.-)
(Beta-1,3-GalTase 7) (Beta3Gal-T7) (b3Gal-T7)
(UDP-galactose:beta-N-acetylglucosamine
beta-1,3-galactosyltransferase 7) (UDP-Gal:beta-GlcNAc
beta-1,3-galactosyltransferase 7) (Beta-3-Gx-T7
Length = 397
Score = 33.1 bits (74), Expect = 1.1
Identities = 48/214 (22%), Positives = 91/214 (42%), Gaps = 15/214 (7%)
Query: 107 EKQKHPTKKVLSVIGVMTTFGRRKNRDAIRKAWMPTGTAMKKLADQKGVIVRFVIGRSAN 166
++ K KK ++ + + R AIR++W + + V+ F++G++
Sbjct: 133 DQPKKCAKKPFLLLAIKSLIPHFARRQAIRESW-----GRETNVGNQTVVRVFLLGKTPP 187
Query: 167 RGD--SLDKEIETEISQTNDFIILDNQVEAPEERAKKIKSFFIYAVDNWDAEFYAKVNDD 224
+ L ++ E + D ++ + + K++ + DAEF K +DD
Sbjct: 188 EDNHPDLSDMLKFESDKHQDILMWNYRDTFFNLSLKEVLFLRWVSTSCPDAEFVFKGDDD 247
Query: 225 VYVNLDALGGVLTSHLDKPR---VYIG-CMKSGQVFSEPTQKWYEPDWWKFGDGKSYFRH 280
V+VN + L S L K + ++IG + + + K+Y P+ + G Y
Sbjct: 248 VFVNTHHILNYLNS-LSKSKAKDLFIGDVIHNAGPHRDKKLKYYIPEVFYTGVYPPY-AG 305
Query: 281 ASGDLYVISKALAQFISINRLILRTYAHDDISTG 314
G LY AL + + +R+ L Y DD+ TG
Sbjct: 306 GGGFLYSGPLALRLYSATSRVHL--YPIDDVYTG 337
>REPB_AGRRH (P05683) Possible replication protein B
Length = 312
Score = 32.7 bits (73), Expect = 1.4
Identities = 32/144 (22%), Positives = 67/144 (46%), Gaps = 15/144 (10%)
Query: 28 ATMATVYVAGRLWQDAENRVHLVEELEKRTGQGQSAVSVDDTLKVIACREQQKKLSALEM 87
AT T + L ++ +R+ EE+E+R +GQ+ + +D + + Q + ++
Sbjct: 14 ATKLTANIGNAL-REQNDRLSRAEEIERRLAEGQAVIELDAS-SIEPSFVQDRMRGDIDG 71
Query: 88 ELTAARKEGFVSKHLSGDNEKQKHPTKKVLSVIGVMTTFGRRKNRDAIRKAWMPTGTAMK 147
LT+ R++G L + HP++ FG R+ R A+ + +P ++
Sbjct: 72 LLTSIREQGQQVPIL-----VRPHPSQPG----RYQVAFGHRRLR-AVSELGLPVRAVVR 121
Query: 148 KLADQKGVIVRFVIGRSANRGDSL 171
+L D++ V+ + G+ N + L
Sbjct: 122 ELTDEQVVVAQ---GQENNEREDL 142
>BRN_DROME (Q24157) Beta-1,3-galactosyltransferase brn (EC 2.4.1.62)
(Neurogenic secreted signaling protein brn) (Brainiac
protein)
Length = 325
Score = 32.7 bits (73), Expect = 1.4
Identities = 52/219 (23%), Positives = 93/219 (41%), Gaps = 26/219 (11%)
Query: 122 VMTTFGRRKNRDAIRKAWMPTGTAMKKLADQKGVIVR--FVIGRSANRGDSLDKEIETEI 179
+ + G + R+AIR+ W G + +D V +R F++G + + +K++ E
Sbjct: 84 IKSAVGNSRRREAIRRTWGYEG----RFSD---VHLRRVFLLGTA----EDSEKDVAWES 132
Query: 180 SQTNDFIILDNQVEAPEERAKKIKSFFIYAVDNWD-AEFYAKVNDDVYVN----LDALGG 234
+ D I+ +A K +A D ++ +EFY V+DD YV+ L LG
Sbjct: 133 REHGD-ILQAEFTDAYFNNTLKTMLGMRWASDQFNRSEFYLFVDDDYYVSAKNVLKFLGR 191
Query: 235 VLTSHLDKPRVYIGCMKSGQVFSEPTQKWY-EPDWWKFGDGKSYFRHASGDLYVISKALA 293
SH ++ G + KWY + + F Y +G + KAL
Sbjct: 192 GRQSH-QPELLFAGHVFQTSPLRHKFSKWYVSLEEYPFDRWPPYV--TAGAFILSQKALR 248
Query: 294 QFISINRLILRTYAHDDISTG--SWFIGLDVMHVDETKF 330
Q + + + L + DD+ G + G+ + H D+ +F
Sbjct: 249 QLYAAS-VHLPLFRFDDVYLGIVALKAGISLQHCDDFRF 286
>M5_STRP5 (P02977) M protein, serotype 5 precursor
Length = 492
Score = 32.0 bits (71), Expect = 2.5
Identities = 36/171 (21%), Positives = 70/171 (40%), Gaps = 17/171 (9%)
Query: 49 LVEELEKRTGQGQSAVSVDDTLKVIACREQQKK--LSALEMELTAARKEGFVS------- 99
+ +E E + G +D+T+K +EQ+ K + AL+ EL + +S
Sbjct: 208 IAKEQENKETIGTLKKILDETVKDKLAKEQKSKQNIGALKQELAKKDEANKISDASRKGL 267
Query: 100 -KHLSGDNEKQK-----HPTKKVLSVIGVMTTFGRRKNRDAIRKAWMPTGTAMKKLADQK 153
+ L E +K H + + I + G R++ DA R+A +KL +Q
Sbjct: 268 RRDLDASREAKKQLEAEHQKLEEQNKISEASRKGLRRDLDASREAKKQLEAEQQKLEEQN 327
Query: 154 GV--IVRFVIGRSANRGDSLDKEIETEISQTNDFIILDNQVEAPEERAKKI 202
+ R + R + K++E + + N + ++ E +KK+
Sbjct: 328 KISEASRKGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKL 378
>AROK_BUCBP (P59488) Shikimate kinase (EC 2.7.1.71) (SK)
Length = 174
Score = 32.0 bits (71), Expect = 2.5
Identities = 27/108 (25%), Positives = 43/108 (39%), Gaps = 16/108 (14%)
Query: 105 DNEKQKHPTKKVLSVIGVMTTFGRRKNRDAIRKAWMPTGTAMKKLADQKGVIVRFVIGRS 164
D E +K V + + G RK I + ++ D++G+++ S
Sbjct: 36 DQEIEKRTGVDVSWIFDIEGELGFRKREQKI----------ISEIIDKRGIVL------S 79
Query: 165 ANRGDSLDKEIETEISQTNDFIILDNQVEAPEERAKKIKSFFIYAVDN 212
G L +EI ++S I L+ VE R KK K + VDN
Sbjct: 80 TGGGSILSREIRNKLSSRGVVIYLETSVEKQLVRTKKNKQRPLLQVDN 127
>LMD1_HUMAN (P29536) Leiomodin 1 (Leiomodin, muscle form) (64 kDa
autoantigen D1) (64 kDa autoantigen 1D) (64 kDa
autoantigen 1D3) (Thyroid-associated ophthalmopathy
autoantigen) (Smooth muscle leiomodin) (SM-Lmod)
Length = 572
Score = 31.6 bits (70), Expect = 3.2
Identities = 24/84 (28%), Positives = 39/84 (45%), Gaps = 6/84 (7%)
Query: 36 AGRLWQDAENRVHLVEELEKRTGQGQSAVSVD-----DTLKVIACREQQKKLSALEMELT 90
AG+ + E V +E EK+ G + +S D + +K +A +E +K+ E T
Sbjct: 144 AGKDGRGEERAVATKKEEEKKGGDRNTGLSRDKDKKREEMKEVAKKEDDEKVKG-ERRNT 202
Query: 91 AARKEGFVSKHLSGDNEKQKHPTK 114
RKEG K G+ + +K K
Sbjct: 203 DTRKEGEKMKRAGGNTDMKKEDEK 226
>YEIT_ECOLI (P76440) Hypothetical oxidoreductase yeiT
Length = 412
Score = 31.2 bits (69), Expect = 4.2
Identities = 22/67 (32%), Positives = 31/67 (45%), Gaps = 13/67 (19%)
Query: 58 GQGQSAVSVDDTLKVIAC-------REQQKKLSALEMELTAARK------EGFVSKHLSG 104
G G A+ V TLKV+ C RE+ + A E E T+AR+ +GF + G
Sbjct: 260 GGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEG 319
Query: 105 DNEKQKH 111
+ KH
Sbjct: 320 NKVTFKH 326
>MP54_YEAST (Q08550) Meiotic plaque component protein 54
Length = 464
Score = 31.2 bits (69), Expect = 4.2
Identities = 21/85 (24%), Positives = 39/85 (45%), Gaps = 11/85 (12%)
Query: 157 VRFVIGRSANRGDSLDKEIETEISQTNDFIILDNQVEAP-----EERAKKIKSFFIYAVD 211
+ F+I +S + + L++ ++T+ + N F Q E P ++R K+K F +
Sbjct: 344 LNFIIDKSMKKIEHLERSLQTQFTAQNSFSTAMIQEEGPKDAHLKDRYHKVKEFMEQKLQ 403
Query: 212 NWDAEFYAKVNDDVYVNLDALGGVL 236
+K+ND +AL VL
Sbjct: 404 T------SKINDPSCSEAEALDNVL 422
>DMD_HUMAN (P11532) Dystrophin
Length = 3685
Score = 30.8 bits (68), Expect = 5.5
Identities = 32/147 (21%), Positives = 64/147 (42%), Gaps = 18/147 (12%)
Query: 51 EELEKRTGQGQSAVSVDDTLKVIACREQ---QKKLSALEMELTA--ARKEGFVS------ 99
+E+EK+ +S V + LK + +++ + KLS L A +R E +++
Sbjct: 1619 KEIEKQKVHLKSITEVGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQ 1678
Query: 100 KHLSGDNEKQKHPTKKVLSVIGVMTTFGRRK---NRDAIRKAWMPTGTAMKKLADQKGVI 156
KH+ ++ H TK ++ ++ ++K D +++ K+ +
Sbjct: 1679 KHMETFDQNVDHITKWIIQADTLLDESEKKKPQQKEDVLKRLKAELNDIRPKVDSTRDQA 1738
Query: 157 VRFVIGRSANRGDSLDKEIETEISQTN 183
+ ANRGD K +E +IS+ N
Sbjct: 1739 ANLM----ANRGDHCRKLVEPQISELN 1761
>MLP2_YEAST (P40457) MLP2 protein (Myosin-like protein 2)
Length = 1679
Score = 30.4 bits (67), Expect = 7.2
Identities = 23/83 (27%), Positives = 45/83 (53%), Gaps = 4/83 (4%)
Query: 100 KHLSGDNEKQKHPTKKVLSVIGVMTTFGRRKNR---DAIRKAWMPTGTAMKKLADQKGVI 156
K L ++ +QK+ K+ L+ + R + R DA++K + AM+++ D+K V
Sbjct: 63 KQLLDESSEQKNTAKEELNGLKDQLNEERSRYRREIDALKKQLHVSHEAMREVNDEKRVK 122
Query: 157 VRFVIGRSANRG-DSLDKEIETE 178
+ I +S ++G DSL+ ++ E
Sbjct: 123 EEYDIWQSRDQGNDSLNDDLNKE 145
>GSPD_ERWCA (P31701) General secretion pathway protein D precursor
(Pectic enzymes secretion protein outD)
Length = 650
Score = 30.4 bits (67), Expect = 7.2
Identities = 19/89 (21%), Positives = 41/89 (45%)
Query: 96 GFVSKHLSGDNEKQKHPTKKVLSVIGVMTTFGRRKNRDAIRKAWMPTGTAMKKLADQKGV 155
G + LS +++ T ++++ + TF + + + +G + + ++K V
Sbjct: 414 GMLMTALSSNSKNDILATPSIVTLDNMEATFNVGQEVPVLAGSQTTSGDNVFQTVERKTV 473
Query: 156 IVRFVIGRSANRGDSLDKEIETEISQTND 184
++ + N GDS+ EIE E+S D
Sbjct: 474 GIKLKVKPQINEGDSVLLEIEQEVSSVAD 502
>PTAA_ECOLI (P09323) PTS system, N-acetylglucosamine-specific IIABC
component (EIIABC-Nag) (N-acetylglucosamine-permease
IIABC component) (Phosphotransferase enzyme II, ABC
component) (EC 2.7.1.69) (EII-Nag)
Length = 648
Score = 30.0 bits (66), Expect = 9.4
Identities = 24/69 (34%), Positives = 33/69 (47%), Gaps = 9/69 (13%)
Query: 280 HASGDLYVISKALAQ--FISINRLILRTYAHDDISTGSWF-IGL------DVMHVDETKF 330
HA G+ V + AL F INRL++ T H ++T +WF IG V H D +F
Sbjct: 160 HAGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRF 219
Query: 331 CCSSRSPGM 339
+ GM
Sbjct: 220 YAGDGTAGM 228
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.133 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,774,427
Number of Sequences: 164201
Number of extensions: 1704571
Number of successful extensions: 4275
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 4271
Number of HSP's gapped (non-prelim): 15
length of query: 358
length of database: 59,974,054
effective HSP length: 111
effective length of query: 247
effective length of database: 41,747,743
effective search space: 10311692521
effective search space used: 10311692521
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0060.1