BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0050.14
(77 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PRS8_DICDI (P34124) 26S protease regulatory subunit 8 (TAT-bindi... 106 1e-23
PRS8_RAT (P62198) 26S protease regulatory subunit 8 (Proteasome ... 104 4e-23
PRS8_PIG (P62197) 26S protease regulatory subunit 8 (Proteasome ... 104 4e-23
PRS8_NAEFO (Q25544) 26S protease regulatory subunit 8 homolog (T... 104 4e-23
PRS8_MOUSE (P62196) 26S protease regulatory subunit 8 (Proteasom... 104 4e-23
PRS8_HUMAN (P62195) 26S protease regulatory subunit 8 (Proteasom... 104 4e-23
PRS8_BOVIN (P62194) 26S protease regulatory subunit 8 (Proteasom... 104 4e-23
PRS8_YEAST (Q01939) 26S protease regulatory subunit 8 homolog (S... 103 1e-22
PRS8_XENLA (P46470) 26S protease regulatory subunit 8 (SUG1 homo... 102 2e-22
PRS8_MANSE (P54814) 26S protease regulatory subunit 8 (18-56 pro... 102 2e-22
PRS8_DROME (O18413) 26S protease regulatory subunit 8 102 2e-22
PRS8_SCHPO (P41836) 26S protease regulatory subunit 8 homolog (P... 101 4e-22
PSMR_METJA (Q58576) Proteasome-activating nucleotidase (Proteaso... 84 9e-17
PSMR_METKA (Q8TX03) Proteasome-activating nucleotidase (Proteaso... 81 4e-16
PSMR_PYRFU (Q8U4H3) Proteasome-activating nucleotidase (Proteaso... 81 6e-16
PSMR_PYRHO (O57940) Proteasome-activating nucleotidase (Proteaso... 80 9e-16
PSMR_PYRAB (Q9V287) Proteasome-activating nucleotidase (Proteaso... 80 9e-16
YC97_ARCFU (O28972) Cell division cycle protein 48 homolog AF1297 80 1e-15
VAT_THEAC (O05209) VCP-like ATPase 80 1e-15
PSMR_METTH (O26824) Proteasome-activating nucleotidase (Proteaso... 79 2e-15
>PRS8_DICDI (P34124) 26S protease regulatory subunit 8 (TAT-binding
protein homolog 10) (Fragment)
Length = 389
Score = 106 bits (264), Expect = 1e-23
Identities = 51/56 (91%), Positives = 53/56 (94%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+PIKHPELFES GIAQPKGVLLYGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 143 IKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSG 198
>PRS8_RAT (P62198) 26S protease regulatory subunit 8 (Proteasome
subunit p45) (p45/SUG) (Proteasome 26S subunit ATPase 5)
(Thyroid hormone receptor interacting protein 1) (TRIP1)
Length = 406
Score = 104 bits (260), Expect = 4e-23
Identities = 49/56 (87%), Positives = 53/56 (94%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPELFE+ GIAQPKGVLLYGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 161 IKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSG 216
>PRS8_PIG (P62197) 26S protease regulatory subunit 8 (Proteasome
subunit p45) (p45/SUG) (Proteasome 26S subunit ATPase 5)
(TAT-binding protein homolog 10) (TBP10)
Length = 406
Score = 104 bits (260), Expect = 4e-23
Identities = 49/56 (87%), Positives = 53/56 (94%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPELFE+ GIAQPKGVLLYGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 161 IKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSG 216
>PRS8_NAEFO (Q25544) 26S protease regulatory subunit 8 homolog
(TAT-binding protein homolog)
Length = 414
Score = 104 bits (260), Expect = 4e-23
Identities = 49/56 (87%), Positives = 53/56 (94%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+PIKHPELFES GIAQPKGV++YGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 168 IKEVIELPIKHPELFESLGIAQPKGVIMYGPPGTGKTLLARAVAHHTDCTFIRVSG 223
>PRS8_MOUSE (P62196) 26S protease regulatory subunit 8 (Proteasome
subunit p45) (p45/SUG) (Proteasome 26S subunit ATPase 5)
(mSUG1)
Length = 406
Score = 104 bits (260), Expect = 4e-23
Identities = 49/56 (87%), Positives = 53/56 (94%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPELFE+ GIAQPKGVLLYGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 161 IKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSG 216
>PRS8_HUMAN (P62195) 26S protease regulatory subunit 8 (Proteasome
subunit p45) (p45/SUG) (Proteasome 26S subunit ATPase 5)
(Thyroid hormone receptor interacting protein 1) (TRIP1)
Length = 406
Score = 104 bits (260), Expect = 4e-23
Identities = 49/56 (87%), Positives = 53/56 (94%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPELFE+ GIAQPKGVLLYGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 161 IKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSG 216
>PRS8_BOVIN (P62194) 26S protease regulatory subunit 8 (Proteasome
subunit p45) (p45/SUG) (Proteasome 26S subunit ATPase 5)
Length = 406
Score = 104 bits (260), Expect = 4e-23
Identities = 49/56 (87%), Positives = 53/56 (94%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPELFE+ GIAQPKGVLLYGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 161 IKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSG 216
>PRS8_YEAST (Q01939) 26S protease regulatory subunit 8 homolog (SUG1
protein) (CIM3 protein) (TAT-binding protein TBY1)
Length = 405
Score = 103 bits (256), Expect = 1e-22
Identities = 49/63 (77%), Positives = 54/63 (84%)
Query: 1 LASCLSYILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIR 60
L + I +VIE+P+KHPELFES GIAQPKGV+LYGPPGT KTLLARAVAHHTDC FIR
Sbjct: 153 LTKQIKEIKEVIELPVKHPELFESLGIAQPKGVILYGPPGTGKTLLARAVAHHTDCKFIR 212
Query: 61 VSG 63
VSG
Sbjct: 213 VSG 215
>PRS8_XENLA (P46470) 26S protease regulatory subunit 8 (SUG1
homolog) (xSUG1)
Length = 461
Score = 102 bits (253), Expect = 2e-22
Identities = 48/56 (85%), Positives = 52/56 (92%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPE FE+ GIAQPKGVLLYGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 156 IKEVIELPVKHPEHFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSG 211
>PRS8_MANSE (P54814) 26S protease regulatory subunit 8 (18-56
protein)
Length = 402
Score = 102 bits (253), Expect = 2e-22
Identities = 47/56 (83%), Positives = 53/56 (93%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPELF++ GIAQPKGVLLYGPPGT KTLLARAVAHHT+CTFIRVSG
Sbjct: 157 IKEVIELPVKHPELFDALGIAQPKGVLLYGPPGTGKTLLARAVAHHTECTFIRVSG 212
>PRS8_DROME (O18413) 26S protease regulatory subunit 8
Length = 405
Score = 102 bits (253), Expect = 2e-22
Identities = 47/56 (83%), Positives = 53/56 (93%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPELF++ GIAQPKGVLLYGPPGT KTLLARAVAHHT+CTFIRVSG
Sbjct: 160 IKEVIELPVKHPELFDALGIAQPKGVLLYGPPGTGKTLLARAVAHHTECTFIRVSG 215
>PRS8_SCHPO (P41836) 26S protease regulatory subunit 8 homolog
(Protein let1)
Length = 403
Score = 101 bits (251), Expect = 4e-22
Identities = 47/56 (83%), Positives = 51/56 (90%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+P+KHPELFES GI QPKG+LLYGPPGT KTLLARAVAHHTDC FIRVSG
Sbjct: 157 IKEVIELPVKHPELFESLGIPQPKGILLYGPPGTGKTLLARAVAHHTDCKFIRVSG 212
>PSMR_METJA (Q58576) Proteasome-activating nucleotidase (Proteasome
regulatory subunit)
Length = 430
Score = 83.6 bits (205), Expect = 9e-17
Identities = 39/56 (69%), Positives = 46/56 (81%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +V+E+P+KHPELFE GI PKG+LLYGPPGT KTLLA+AVA T+ TFIRV G
Sbjct: 182 IREVVELPLKHPELFEKVGIEPPKGILLYGPPGTGKTLLAKAVATETNATFIRVVG 237
>PSMR_METKA (Q8TX03) Proteasome-activating nucleotidase (Proteasome
regulatory subunit)
Length = 436
Score = 81.3 bits (199), Expect = 4e-16
Identities = 37/55 (67%), Positives = 45/55 (81%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVS 62
I +V+E P+K PELFE G+ PKGVLLYGPPGT KTLLA+AVA+H D TFIR++
Sbjct: 191 IREVVEKPLKEPELFEKVGVEPPKGVLLYGPPGTGKTLLAKAVANHADATFIRLA 245
>PSMR_PYRFU (Q8U4H3) Proteasome-activating nucleotidase (Proteasome
regulatory subunit)
Length = 396
Score = 80.9 bits (198), Expect = 6e-16
Identities = 37/59 (62%), Positives = 45/59 (75%)
Query: 5 LSYILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
L + + IE+P+KHPELFE GI PKGVLLYGPPG KTL+A+A+AH + TFIRV G
Sbjct: 146 LQELREAIELPLKHPELFEEVGIDPPKGVLLYGPPGCGKTLMAKAIAHEVNATFIRVVG 204
>PSMR_PYRHO (O57940) Proteasome-activating nucleotidase (Proteasome
regulatory subunit)
Length = 399
Score = 80.1 bits (196), Expect = 9e-16
Identities = 37/59 (62%), Positives = 45/59 (75%)
Query: 5 LSYILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
L + + IE+P+KHPELFE GI PKGVLLYGPPG KTL+A+A+AH + TFIRV G
Sbjct: 149 LQELREAIELPLKHPELFEEVGIDPPKGVLLYGPPGCGKTLMAKALAHEVNATFIRVVG 207
>PSMR_PYRAB (Q9V287) Proteasome-activating nucleotidase (Proteasome
regulatory subunit)
Length = 399
Score = 80.1 bits (196), Expect = 9e-16
Identities = 37/59 (62%), Positives = 45/59 (75%)
Query: 5 LSYILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
L + + IE+P+KHPELFE GI PKGVLLYGPPG KTL+A+A+AH + TFIRV G
Sbjct: 149 LQELREAIELPLKHPELFEEVGIDPPKGVLLYGPPGCGKTLMAKALAHEVNATFIRVVG 207
>YC97_ARCFU (O28972) Cell division cycle protein 48 homolog AF1297
Length = 733
Score = 79.7 bits (195), Expect = 1e-15
Identities = 40/74 (54%), Positives = 52/74 (70%), Gaps = 1/74 (1%)
Query: 5 LSYILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG- 63
L + ++IE+P+KHPELF+ GI PKGVLLYGPPGT KTL+A+AVA+ D FI +SG
Sbjct: 191 LRLVREMIELPLKHPELFQRLGIEPPKGVLLYGPPGTGKTLIAKAVANEVDAHFIPISGP 250
Query: 64 KVYSLYSASSTHYL 77
++ S Y S L
Sbjct: 251 EIMSKYYGESEQRL 264
Score = 67.8 bits (164), Expect = 5e-12
Identities = 28/56 (50%), Positives = 43/56 (76%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
+++ +E P+K+PE+F + I P+G+LL+GPPGT KTLLA+AVA+ ++ FI V G
Sbjct: 467 LMEAVEWPLKYPEVFRAANIKPPRGILLFGPPGTGKTLLAKAVANESNANFISVKG 522
>VAT_THEAC (O05209) VCP-like ATPase
Length = 745
Score = 79.7 bits (195), Expect = 1e-15
Identities = 40/78 (51%), Positives = 54/78 (68%), Gaps = 1/78 (1%)
Query: 1 LASCLSYILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIR 60
L+ L I ++IE+P+KHPELFE GI PKGV+LYGPPGT KTL+ARAVA+ + F+
Sbjct: 195 LSEQLGKIREMIELPLKHPELFERLGITPPKGVILYGPPGTGKTLIARAVANESGANFLS 254
Query: 61 VSG-KVYSLYSASSTHYL 77
++G ++ S Y S L
Sbjct: 255 INGPEIMSKYYGQSEQKL 272
Score = 59.3 bits (142), Expect = 2e-09
Identities = 27/56 (48%), Positives = 38/56 (67%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I + +E+P+ P++F+ GI KG LLYGPPG KTLLA+AVA ++ FI + G
Sbjct: 479 IKETVELPLLKPDVFKRLGIRPSKGFLLYGPPGVGKTLLAKAVATESNANFISIKG 534
>PSMR_METTH (O26824) Proteasome-activating nucleotidase (Proteasome
regulatory subunit)
Length = 410
Score = 79.0 bits (193), Expect = 2e-15
Identities = 35/54 (64%), Positives = 44/54 (80%)
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRV 61
+ + +E+P+K PELFE GI PKGVLLYGPPGT KTLLA+AVAH T+ TFI++
Sbjct: 163 VKETVELPLKKPELFEKIGIEPPKGVLLYGPPGTGKTLLAKAVAHETNATFIKI 216
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.138 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,676,081
Number of Sequences: 164201
Number of extensions: 339919
Number of successful extensions: 1717
Number of sequences better than 10.0: 531
Number of HSP's better than 10.0 without gapping: 511
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 1154
Number of HSP's gapped (non-prelim): 572
length of query: 77
length of database: 59,974,054
effective HSP length: 53
effective length of query: 24
effective length of database: 51,271,401
effective search space: 1230513624
effective search space used: 1230513624
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0050.14