BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0046a.3
         (160 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

KUP_ENTFA (Q837G9) Probable potassium transport system protein kup     32  0.61
SL54_PIG (P31636) Low affinity sodium-glucose cotransporter (Sod...    32  0.80
M4A3_HUMAN (Q96HJ5) Membrane-spanning 4-domains subfamily A memb...    31  1.0
KUP1_LACPL (Q88Z42) Probable potassium transport system protein ...    31  1.0
M040_ARATH (P93278) Hypothetical ATP synthase C chain-like prote...    31  1.4
SL54_HUMAN (Q9NY91) Low affinity sodium-glucose cotransporter (S...    29  4.0
NU3M_DINSE (O79553) NADH-ubiquinone oxidoreductase chain 3 (EC 1...    29  4.0
SL54_MOUSE (Q9ET37) Low affinity sodium-glucose cotransporter (S...    28  8.9
SL51_HUMAN (P13866) Sodium/glucose cotransporter 1 (Na(+)/glucos...    28  8.9

>KUP_ENTFA (Q837G9) Probable potassium transport system protein kup
          Length = 668

 Score = 32.0 bits (71), Expect = 0.61
 Identities = 20/70 (28%), Positives = 31/70 (43%), Gaps = 6/70 (8%)

Query: 16  LNFCMYVIVLGIGGWAMNRAIDQGFIIGPELQLPAHFSPIFFPMGNASTGFFVTFALIAG 75
           +  C+ +  LG   W +    +      PE+Q  A  +P F  +      F V FA IA 
Sbjct: 258 IKICLILNYLGQAAWLLTVKEN------PEMQALAEINPFFQMIPRGILVFGVVFATIAA 311

Query: 76  VVGAASAISG 85
           V+ + + ISG
Sbjct: 312 VIASQALISG 321


>SL54_PIG (P31636) Low affinity sodium-glucose cotransporter
           (Sodium/glucose cotransporter 3) (Na(+)/glucose
           cotransporter 3)
          Length = 660

 Score = 31.6 bits (70), Expect = 0.80
 Identities = 35/142 (24%), Positives = 55/142 (38%), Gaps = 31/142 (21%)

Query: 21  YVIVLGIGGWAMNRAIDQGFIIGPELQLPAHFSPIFFPMGNASTGFFVTFALIAGVVGAA 80
           +V+V+ +G WAM R  ++G + G            FF  G   T + +  +L A  +G+ 
Sbjct: 36  FVVVMAVGLWAMLRT-NRGTVGG------------FFLAGRDVTWWPMGASLFASNIGS- 81

Query: 81  SAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWKEVELRIRNARLKTMEAFL-- 138
                  H          S  ++A   W   LL +   W  V + I+ A + TM  +L  
Sbjct: 82  ------GHFVGLAGTGAASGIAIAAFEWNALLLLLVLGWFFVPIYIK-AGVMTMPEYLRK 134

Query: 139 --------IILSATQLFYIAAI 152
                   I LS   LF   A+
Sbjct: 135 RFGGKRLQIYLSILSLFICVAL 156


>M4A3_HUMAN (Q96HJ5) Membrane-spanning 4-domains subfamily A member
           3 (Hematopoietic-specific transmembrane-4 protein)
           (HTm4) (CD20 antigen-like protein)
          Length = 214

 Score = 31.2 bits (69), Expect = 1.0
 Identities = 26/92 (28%), Positives = 40/92 (43%), Gaps = 6/92 (6%)

Query: 39  GFIIGPELQLPAHFSPIFFPMGNASTGFFVTFALIAGVVGAASAISGINHIRSWTAESLP 98
           G  +G  LQ P HF   FF      TG+ +  A+     G  S ++GI   R+W   S  
Sbjct: 66  GVFLG-SLQYPYHFQKHFF-FFTFYTGYPIWGAVFFCSSGTLSVVAGIKPTRTWIQNSFG 123

Query: 99  SAASVATMAWTLTLLAMGFAWKEVELRIRNAR 130
              + AT+A    L+   F    + + I++ R
Sbjct: 124 MNIASATIA----LVGTAFLSLNIAVNIQSLR 151


>KUP1_LACPL (Q88Z42) Probable potassium transport system protein
           kup1
          Length = 660

 Score = 31.2 bits (69), Expect = 1.0
 Identities = 15/46 (32%), Positives = 25/46 (53%)

Query: 40  FIIGPELQLPAHFSPIFFPMGNASTGFFVTFALIAGVVGAASAISG 85
           +I+GP   +  H +P F  +  + T   V FA +A ++ + S ISG
Sbjct: 269 WILGPGQAIQGHVNPFFAMLPASLTMAAVIFATLAAIIASQSLISG 314


>M040_ARATH (P93278) Hypothetical ATP synthase C chain-like protein
           (ORF315)
          Length = 315

 Score = 30.8 bits (68), Expect = 1.4
 Identities = 26/85 (30%), Positives = 38/85 (44%), Gaps = 5/85 (5%)

Query: 74  AGVVGAASAISGINHIRSWTAESL---PSAASVATMAWTLTLLAMGFAWKEVELRIRNAR 130
           A  + +A A  GI ++ S    S+   PS A+   +  TLTLL    A+     R++   
Sbjct: 23  AATIASAGAAIGIGNVFSSLIHSVARNPSLATTTVLVVTLTLLGGVAAFYLHSFRLKGPL 82

Query: 131 LKTMEAFLIILSAT--QLFYIAAIH 153
            K +  FL+   A    L  I AIH
Sbjct: 83  KKIIYLFLVFFIAVGISLIRIKAIH 107


>SL54_HUMAN (Q9NY91) Low affinity sodium-glucose cotransporter
           (Sodium/glucose cotransporter 3) (Na(+)/glucose
           cotransporter 3)
          Length = 659

 Score = 29.3 bits (64), Expect = 4.0
 Identities = 28/121 (23%), Positives = 49/121 (40%), Gaps = 27/121 (22%)

Query: 21  YVIVLGIGGWAM---NRAIDQGFIIGPELQLPAHFSPIFFPMGNASTGFFVTFALIAGVV 77
           +++V+ +G WAM   NR    GF +       A     ++PMG +         L A  +
Sbjct: 36  FLVVMAVGLWAMLKTNRGTIGGFFL-------AGRDMAWWPMGAS---------LFASNI 79

Query: 78  GAASAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWKEVELRIRNARLKTMEAF 137
           G+       NH          S  +  T  WT +++ +   W  V + I++  + TM  +
Sbjct: 80  GS-------NHYVGLAGTGAASGVATVTFEWTSSVMLLILGWIFVPIYIKSG-VMTMPEY 131

Query: 138 L 138
           L
Sbjct: 132 L 132


>NU3M_DINSE (O79553) NADH-ubiquinone oxidoreductase chain 3 (EC
           1.6.5.3)
          Length = 114

 Score = 29.3 bits (64), Expect = 4.0
 Identities = 25/104 (24%), Positives = 46/104 (44%), Gaps = 3/104 (2%)

Query: 20  MYVIVLGIGGWAMNRA---IDQGFIIGPELQLPAHFSPIFFPMGNASTGFFVTFALIAGV 76
           M +I L I   AM  A   I+    + P++   + +   F P+GNA T   + F L+A +
Sbjct: 1   MNLITLIIMAMAMTTALYTINTYTTMKPDINKLSPYECGFDPLGNARTPISIQFFLVAIL 60

Query: 77  VGAASAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWK 120
                    +     W+  + PS  ++  +   LT+L +G  ++
Sbjct: 61  FILFDLEIVLLLPTPWSMNTNPSNTTILLITMLLTILTLGLLYE 104


>SL54_MOUSE (Q9ET37) Low affinity sodium-glucose cotransporter
          (Sodium/glucose cotransporter 3) (Na(+)/glucose
          cotransporter 3)
          Length = 656

 Score = 28.1 bits (61), Expect = 8.9
 Identities = 25/73 (34%), Positives = 35/73 (47%), Gaps = 20/73 (27%)

Query: 21 YVIVLGIGGWAM---NRAIDQGFIIGPELQLPAHFSPIFFPMG------NASTGFFVTFA 71
          +V+V+ +G WAM   NR+   GF +       A  S  ++PMG      N  +G FV  A
Sbjct: 36 FVVVMAVGVWAMLKTNRSTVGGFFL-------AGRSMTWWPMGASLFASNIGSGHFVGLA 88

Query: 72 LIAGVVGAASAIS 84
                GAAS I+
Sbjct: 89 ----GTGAASGIA 97


>SL51_HUMAN (P13866) Sodium/glucose cotransporter 1 (Na(+)/glucose
           cotransporter 1) (High affinity sodium-glucose
           cotransporter)
          Length = 664

 Score = 28.1 bits (61), Expect = 8.9
 Identities = 31/127 (24%), Positives = 51/127 (39%), Gaps = 39/127 (30%)

Query: 21  YVIVLGIGGWAM---NRAIDQGFIIGPELQLPAHFSPIFFPMG------NASTGFFVTFA 71
           +V+V+ +G WAM   NR    GF +       A  S +++P+G      N  +G FV   
Sbjct: 36  FVVVMAVGLWAMFSTNRGTVGGFFL-------AGRSMVWWPIGASLFASNIGSGHFV--- 85

Query: 72  LIAGVVGAASAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWKEVELRIRNARL 131
              G+ G  +A                S  ++    W   +L +   W  V + I+ A +
Sbjct: 86  ---GLAGTGAA----------------SGIAIGGFEWNALVLVVVLGWLFVPIYIK-AGV 125

Query: 132 KTMEAFL 138
            TM  +L
Sbjct: 126 VTMPEYL 132


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.328    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,652,667
Number of Sequences: 164201
Number of extensions: 605684
Number of successful extensions: 1860
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1857
Number of HSP's gapped (non-prelim): 9
length of query: 160
length of database: 59,974,054
effective HSP length: 101
effective length of query: 59
effective length of database: 43,389,753
effective search space: 2559995427
effective search space used: 2559995427
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 61 (28.1 bits)