BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0045.10
(257 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
OSA_DROME (Q8IN94) Trithorax group protein OSA (Eyelid protein) 36 0.10
MRC1_YEAST (P25588) Mediator of replication checkpoint protein 1... 33 0.88
GRPE_PSEAE (Q9HV42) GrpE protein (HSP-70 cofactor) 32 1.5
HD_FUGRU (P51112) Huntingtin (Huntington'S disease protein homol... 31 2.6
YPFI_ECOLI (P76562) Hypothetical protein ypfI 31 3.4
FRA1_RAT (P10158) Fos-related antigen 1 (FRA-1) 30 4.4
FRA1_MOUSE (P48755) Fos-related antigen-1 (FRA-1) 30 4.4
LEU1_BRUSU (Q8FZC4) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 30 5.7
C5BA_BACTU (Q45712) Pesticidial crystal protein cry5Ba (Insectic... 30 5.7
KJ85_HUMAN (Q8TF17) Protein KIAA1985 (PP12494) 30 7.5
PERL_HUMAN (P22079) Lactoperoxidase precursor (EC 1.11.1.7) (LPO... 29 9.8
LTB2_HUMAN (Q14767) Latent transforming growth factor-beta-bindi... 29 9.8
FRA1_HUMAN (P15407) Fos-related antigen 1 (FRA-1) 29 9.8
CARA_WIGBR (Q8D3H7) Carbamoyl-phosphate synthase small chain (EC... 29 9.8
>OSA_DROME (Q8IN94) Trithorax group protein OSA (Eyelid protein)
Length = 2716
Score = 35.8 bits (81), Expect = 0.10
Identities = 22/65 (33%), Positives = 28/65 (42%), Gaps = 4/65 (6%)
Query: 67 PDWLPFLPGYSFWVPPRPSPSSVVHLAHRFNSSDQ--PQDALNLESHHGWPDPNYFLQGN 124
P P PG PP P P++V+H H Q P + + HHG P P G
Sbjct: 68 PYGAPPPPGSGPGGPPGPDPAAVMHYHHLHQQQQQHPPPPHMQQQQHHGGPAPP--PPGG 125
Query: 125 APAHS 129
AP H+
Sbjct: 126 APEHA 130
>MRC1_YEAST (P25588) Mediator of replication checkpoint protein 1
(DNA replication checkpoint mediator MRC1)
Length = 1096
Score = 32.7 bits (73), Expect = 0.88
Identities = 32/107 (29%), Positives = 48/107 (43%), Gaps = 12/107 (11%)
Query: 81 PPRPSP-----SSVVHLAHRFNSSDQPQDALNLESHHGWPDPNYFLQGNAPAHSGESGVE 135
P +P P S LA +SS D + LE+ H P +Q + H +S VE
Sbjct: 316 PQKPIPTKKFFSKESFLADFDDSSSNEDDDIKLENAHPKP-----VQNDDELHENKS-VE 369
Query: 136 LNLPEEGTVKVKRVLLWLSDILSVGKRIVNYQLCRSMNYEEHKTPKG 182
LNL +E + KRV L LS + KR ++ C +++ + G
Sbjct: 370 LNLTDETRINEKRVPL-LSSYANNLKREIDSSKCITLDLDSDSDEYG 415
>GRPE_PSEAE (Q9HV42) GrpE protein (HSP-70 cofactor)
Length = 186
Score = 32.0 bits (71), Expect = 1.5
Identities = 14/28 (50%), Positives = 19/28 (67%)
Query: 47 EVTLKMFDDLIQRILVKKATPDWLPFLP 74
E+TLKMFDD ++R V+ P+ PF P
Sbjct: 103 ELTLKMFDDTLRRYQVEALNPEGEPFNP 130
>HD_FUGRU (P51112) Huntingtin (Huntington'S disease protein homolog)
(HD protein)
Length = 3148
Score = 31.2 bits (69), Expect = 2.6
Identities = 33/114 (28%), Positives = 47/114 (40%), Gaps = 17/114 (14%)
Query: 129 SGESGVELNLPEEG---------TVKVKRVLLWLSDILSVGKRIVNYQLCRSMNYEEHKT 179
SG SG+E P EG T VLLW +L + NY S E H+T
Sbjct: 1812 SGHSGIEF-YPLEGLNSMVHCLITTHPSLVLLWCQVLLIID--YTNY----SWWTEVHQT 1864
Query: 180 PKGIFMVQPKLMEKSKNTKGKNRGGDREGQFVQMEIPADTALVVVVELTLLTLH 233
PKG + KL+ + +G+ + R + EI AL++ + LH
Sbjct: 1865 PKGHSLSCTKLLSPHSSGEGEEKPETRLA-MINREIVRRGALILFCDYVCQNLH 1917
>YPFI_ECOLI (P76562) Hypothetical protein ypfI
Length = 671
Score = 30.8 bits (68), Expect = 3.4
Identities = 22/71 (30%), Positives = 32/71 (44%), Gaps = 14/71 (19%)
Query: 39 LTEIDAEHEVTLKMFDDLIQRILVKKATPDWL--------PFLPGYSFWVPPRP------ 84
+ E+ A H +T +M + I+R+LV W LPG W+ PRP
Sbjct: 1 MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFEHTLKLRDALPGDWLWISPRPDAENHC 60
Query: 85 SPSSVVHLAHR 95
SPS++ L R
Sbjct: 61 SPSALQTLLGR 71
>FRA1_RAT (P10158) Fos-related antigen 1 (FRA-1)
Length = 275
Score = 30.4 bits (67), Expect = 4.4
Identities = 15/41 (36%), Positives = 16/41 (38%)
Query: 66 TPDWLPFLPGYSFWVPPRPSPSSVVHLAHRFNSSDQPQDAL 106
TP PF P F P P P S H +S D D L
Sbjct: 227 TPSLTPFTPSLVFTYPSTPEPCSSAHRKSSSSSGDPSSDPL 267
>FRA1_MOUSE (P48755) Fos-related antigen-1 (FRA-1)
Length = 273
Score = 30.4 bits (67), Expect = 4.4
Identities = 15/41 (36%), Positives = 16/41 (38%)
Query: 66 TPDWLPFLPGYSFWVPPRPSPSSVVHLAHRFNSSDQPQDAL 106
TP PF P F P P P S H +S D D L
Sbjct: 225 TPSLTPFTPSLVFTYPSTPEPCSSAHRKSSSSSGDPSSDPL 265
>LEU1_BRUSU (Q8FZC4) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 555
Score = 30.0 bits (66), Expect = 5.7
Identities = 18/66 (27%), Positives = 29/66 (43%)
Query: 101 QPQDALNLESHHGWPDPNYFLQGNAPAHSGESGVELNLPEEGTVKVKRVLLWLSDILSVG 160
QP + HH +PDP + A ++GV + +GT + + LS L V
Sbjct: 437 QPDARIKFVDHHTYPDPEQKGRRILTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVK 496
Query: 161 KRIVNY 166
+V+Y
Sbjct: 497 MSVVDY 502
>C5BA_BACTU (Q45712) Pesticidial crystal protein cry5Ba
(Insecticidal delta-endotoxin CryVB(a)) (Crystaline
entomocidal protoxin) (140 kDa crystal protein)
Length = 1245
Score = 30.0 bits (66), Expect = 5.7
Identities = 25/103 (24%), Positives = 45/103 (43%), Gaps = 6/103 (5%)
Query: 50 LKMFDDLIQRILVKKATPDWLPFLPGYSFWVPPRPSPSSVVHLAHRFNSSDQPQDALNLE 109
L +F + IQ + + +TP+ + F + P +P + +A RF++++ E
Sbjct: 151 LGLFQNAIQVAICQGSTPERVNFDQNCTPCNPNQPCKDDLDRVASRFDTANSQFTQHLPE 210
Query: 110 SHHGWPDPNYFLQGNAPAHSGESGVELNLPEEGTVKVKRVLLW 152
+ W D N+ + VEL LP TV +LL+
Sbjct: 211 FKNPWSDE------NSTQEFKRTSVELTLPMYTTVATLHLLLY 247
>KJ85_HUMAN (Q8TF17) Protein KIAA1985 (PP12494)
Length = 1288
Score = 29.6 bits (65), Expect = 7.5
Identities = 20/67 (29%), Positives = 33/67 (48%), Gaps = 6/67 (8%)
Query: 106 LNLESHHGWPDPNYFLQGNAPAHSGESGVELNLPEEGTVKVKRVLLWLSDILSVGKRIVN 165
L+L+S P NY LQ + EL +E +++ +V LWL+ +L G ++ +
Sbjct: 885 LSLKSWAQHPARNYLLQ------AVRLYCELQASKETDMELVQVFLWLAQVLVSGHQLTH 938
Query: 166 YQLCRSM 172
LC M
Sbjct: 939 GLLCYEM 945
>PERL_HUMAN (P22079) Lactoperoxidase precursor (EC 1.11.1.7) (LPO)
(Salivary peroxidase) (SPO)
Length = 712
Score = 29.3 bits (64), Expect = 9.8
Identities = 29/111 (26%), Positives = 48/111 (43%), Gaps = 14/111 (12%)
Query: 19 SSNSMAVCHRHRSSRATKATLTEIDAE--HEVTLKMFDDLIQRILVKKATP--------D 68
+S+++ + +R +R K + D E ++ K+ +Q I + P
Sbjct: 381 TSHTLFLREHNRLARELKRLNPQWDGEKLYQEARKILGAFVQIITFRDYLPILLGDHMQK 440
Query: 69 WLPFLPGYSFWVPPRPSPSSVVHLAHRFNSSDQPQDALNL-ESHHGW-PDP 117
W+P GYS V PR S+V A RF + P L E++ W P+P
Sbjct: 441 WIPPYQGYSESVDPR--ISNVFTFAFRFGHLEVPSSMFRLDENYQPWGPEP 489
>LTB2_HUMAN (Q14767) Latent transforming growth factor-beta-binding
protein 2 precursor (LTBP-2)
Length = 1821
Score = 29.3 bits (64), Expect = 9.8
Identities = 14/35 (40%), Positives = 16/35 (45%), Gaps = 10/35 (28%)
Query: 62 VKKATPDWLPFLPGYSFW----------VPPRPSP 86
V+ P WLP PG+S W PPRP P
Sbjct: 507 VETRPPPWLPASPGHSLWDSNNIPARSGEPPRPLP 541
>FRA1_HUMAN (P15407) Fos-related antigen 1 (FRA-1)
Length = 271
Score = 29.3 bits (64), Expect = 9.8
Identities = 14/41 (34%), Positives = 16/41 (38%)
Query: 66 TPDWLPFLPGYSFWVPPRPSPSSVVHLAHRFNSSDQPQDAL 106
TP PF P F P P P + H +S D D L
Sbjct: 223 TPSLTPFTPSLVFTYPSTPEPCASAHRKSSSSSGDPSSDPL 263
>CARA_WIGBR (Q8D3H7) Carbamoyl-phosphate synthase small chain (EC
6.3.5.5) (Carbamoyl-phosphate synthetase glutamine
chain)
Length = 376
Score = 29.3 bits (64), Expect = 9.8
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Query: 116 DPNYFLQGNAPA--HSGESGVELNLPEEGTVKVKRVLLW 152
DP+YF Q A H G G+E+N E ++VK V+L+
Sbjct: 49 DPSYFNQIVAFTYPHIGNIGIEINNNESSKIQVKAVILY 87
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.135 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,259,891
Number of Sequences: 164201
Number of extensions: 1399035
Number of successful extensions: 2673
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 2667
Number of HSP's gapped (non-prelim): 14
length of query: 257
length of database: 59,974,054
effective HSP length: 108
effective length of query: 149
effective length of database: 42,240,346
effective search space: 6293811554
effective search space used: 6293811554
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0045.10