Lotus
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0043b.5
         (298 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

G143_HUMAN (P51810) G protein-coupled receptor 143 (Ocular albin...    32  1.9
RPC2_CYACA (Q9TM34) DNA-directed RNA polymerase beta'' chain (EC...    31  4.2
DACA_HAEIN (P44466) Penicillin-binding protein 5 precursor (D-al...    31  4.2
RPC2_ADICA (Q85FM9) DNA-directed RNA polymerase beta'' chain (EC...    30  5.5
PBPA_NEILA (O87579) Penicillin-binding protein 1A (PBP-1a) (PBP1...    30  5.5
PBPA_NEIFL (O87626) Penicillin-binding protein 1A (PBP-1a) (PBP1...    30  9.5

>G143_HUMAN (P51810) G protein-coupled receptor 143 (Ocular albinism
           type 1 protein)
          Length = 404

 Score = 32.0 bits (71), Expect = 1.9
 Identities = 22/86 (25%), Positives = 39/86 (44%), Gaps = 16/86 (18%)

Query: 5   VQFSTVRTLLIMCPQTNTHRSSGETVDWISDAINGGSLRHVDLHTGTNGWASPPGDLFSL 64
           ++F  +  +LI+C              W+S+ IN   L ++++ T  NG +  P  + + 
Sbjct: 244 IRFFKIMLVLIIC--------------WLSNIINESLLFYLEMQTDINGGSLKP--VRTA 287

Query: 65  RSTNYFTKRQKSPAGNYLLSPAGMDW 90
             T +F     +PA  +LLS A   W
Sbjct: 288 AKTTWFIMGILNPAQGFLLSLAFYGW 313


>RPC2_CYACA (Q9TM34) DNA-directed RNA polymerase beta'' chain (EC
           2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
           subunit) (RNA polymerase beta'' subunit)
          Length = 1269

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 23/83 (27%), Positives = 38/83 (45%), Gaps = 12/83 (14%)

Query: 178 IVKKAVGNYSACLLGKALTCNYHRG-------SNYLEIDVDIGSSAIANAILH-----LA 225
           +V+ +      CLL KAL  + H G       +NY  ID+ +G+  I NA L+       
Sbjct: 501 VVQNSFEVNKGCLLAKALIKSRHSGISRIRCAANYYAIDIILGTVTIRNANLYKDSKLFN 560

Query: 226 LGYVTAVTIDMGFVIEAQAEEEL 248
             Y+   +    FV++A   ++L
Sbjct: 561 NNYLLKTSNKKNFVVKALVGQKL 583


>DACA_HAEIN (P44466) Penicillin-binding protein 5 precursor
           (D-alanyl-D-alanine carboxypeptidase fraction A) (EC
           3.4.16.4) (DD-peptidase) (DD-carboxypeptidase) (PBP-5)
          Length = 393

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 20/95 (21%), Positives = 46/95 (48%), Gaps = 9/95 (9%)

Query: 126 IPGGKDHHSAVFYFATEEPIQPGSLLDRFVNGDDGFRNQ---RFKLVNRIVKGPWIVKKA 182
           +  GK+      Y+  +  ++ G+L+D F+    G +++   R++L ++ ++ P +  + 
Sbjct: 288 LEAGKEISEQRVYYGDKNSVKLGALMDHFITIPKGKQSEVKARYELADKNLQAPLVKGQV 347

Query: 183 VGNYSACLLGKALTCNYHRGSNYLEIDVDIGSSAI 217
           +G     L GK +       S  L++  D+G + I
Sbjct: 348 IGKVVYQLDGKDI------ASANLQVMNDVGEAGI 376


>RPC2_ADICA (Q85FM9) DNA-directed RNA polymerase beta'' chain (EC
           2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
           subunit) (RNA polymerase beta'' subunit)
          Length = 1420

 Score = 30.4 bits (67), Expect = 5.5
 Identities = 24/77 (31%), Positives = 37/77 (47%), Gaps = 9/77 (11%)

Query: 67  TNYFTKRQKSPAGNYLLSPAGMDWLKSTAKLENVLARADNRVSNALRTRKSFIFAVNLQI 126
           + YF+   K P  NY+LS     WL+  A+LEN ++    R    +  +K    ++N+Q+
Sbjct: 540 SKYFSNWSKRPRSNYILSNV---WLE-RAELENSVSLLMERCQKNI--KKLDFVSINVQL 593

Query: 127 PGGKDHHSAVFYFATEE 143
             G D       FAT E
Sbjct: 594 NNGSDQD---HIFATYE 607


>PBPA_NEILA (O87579) Penicillin-binding protein 1A (PBP-1a) (PBP1a)
           [Includes: Penicillin-insensitive transglycosylase (EC
           2.4.2.-) (Peptidoglycan TGase); Penicillin-sensitive
           transpeptidase (EC 3.4.-.-) (DD-transpeptidase)]
          Length = 798

 Score = 30.4 bits (67), Expect = 5.5
 Identities = 29/97 (29%), Positives = 41/97 (41%), Gaps = 11/97 (11%)

Query: 128 GGKDHHSAVFYFATEEPIQPGSLLDRFV------NGDDGFRNQRFKLVNRIVKGP----W 177
           GG D HS  F  AT+   QPGS    FV       G           ++   KGP    W
Sbjct: 440 GGYDFHSKTFNRATQAMRQPGSTFKPFVYSAALSKGMTASTMINDAPISLPGKGPNGSVW 499

Query: 178 IVKKAVGNYSACL-LGKALTCNYHRGSNYLEIDVDIG 213
             K + G YS  + L +ALT + +  S  + + + +G
Sbjct: 500 TPKNSDGRYSGYITLRQALTASKNMVSIRILMSIGVG 536


>PBPA_NEIFL (O87626) Penicillin-binding protein 1A (PBP-1a) (PBP1a)
           [Includes: Penicillin-insensitive transglycosylase (EC
           2.4.2.-) (Peptidoglycan TGase); Penicillin-sensitive
           transpeptidase (EC 3.4.-.-) (DD-transpeptidase)]
          Length = 798

 Score = 29.6 bits (65), Expect = 9.5
 Identities = 35/153 (22%), Positives = 58/153 (37%), Gaps = 24/153 (15%)

Query: 128 GGKDHHSAVFYFATEEPIQPGSLLDRFV---------NGDDGFRNQRFKLVNRIVKG-PW 177
           GG D+HS  F  AT+   QPGS    F+                +    L  +   G  W
Sbjct: 439 GGYDYHSKTFNRATQAMRQPGSTFKPFIYSAALAKGMTASTMINDAPISLPGKGANGKAW 498

Query: 178 IVKKAVGNYSACL-LGKALTCNYHRGSNYLEIDVDIG-------------SSAIANAILH 223
             K + G Y+  + L +ALT + +  S  + + + IG             S   A+  + 
Sbjct: 499 NPKNSDGRYAGYITLRQALTASKNMVSIRILMSIGIGYAQQYIQRFGFKPSEIPASLSMA 558

Query: 224 LALGYVTAVTIDMGFVIEAQAEEELPERLIGAV 256
           L  G  T + I  G+ + A    ++   +I  +
Sbjct: 559 LGAGETTPLRIAEGYSVFANGGYKVSAHVIDKI 591


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.318    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,735,773
Number of Sequences: 164201
Number of extensions: 1496122
Number of successful extensions: 2871
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 2871
Number of HSP's gapped (non-prelim): 6
length of query: 298
length of database: 59,974,054
effective HSP length: 109
effective length of query: 189
effective length of database: 42,076,145
effective search space: 7952391405
effective search space used: 7952391405
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 65 (29.6 bits)


Lotus: description of TM0043b.5