BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0043b.1
(188 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
EVPL_HUMAN (Q92817) Envoplakin (210 kDa paraneoplastic pemphigus... 33 0.39
INVO_HUMAN (P07476) Involucrin 33 0.51
C190_DROME (Q9VJE5) Restin homolog (Cytoplasmic linker protein 1... 32 1.1
AXN2_RAT (O70240) Axin 2 (Axis inhibition protein 2) (Conductin)... 32 1.1
YHA2_YEAST (P38753) Hypothetical 51.2 kDa protein in LAG1-RPL14B... 31 1.5
Y983_AQUAE (O67109) Hypothetical protein AQ_983 31 1.5
NKX1_HUMAN (O60721) Sodium/potassium/calcium exchanger 1 precurs... 31 1.5
MENE_BACCR (Q816I1) O-succinylbenzoate--CoA ligase (EC 6.2.1.26)... 31 1.9
MENE_BACAN (Q81K97) O-succinylbenzoate--CoA ligase (EC 6.2.1.26)... 31 1.9
SSXT_MOUSE (Q62280) SSXT protein (SYT protein) (Synovial sarcoma... 30 2.5
INVO_PONPY (P14708) Involucrin 30 2.5
ATF5_HUMAN (Q9Y2D1) Cyclic-AMP-dependent transcription factor AT... 30 2.5
MX1_RAT (P18588) Interferon-induced GTP-binding protein Mx1 30 3.3
K179_HUMAN (Q14684) Protein KIAA0179 30 3.3
INVO_PANPA (P14591) Involucrin 30 3.3
SSXT_HUMAN (Q15532) SSXT protein (Synovial sarcoma, translocated... 30 4.3
IF2_AQUAE (O67825) Translation initiation factor IF-2 30 4.3
DJLA_PHOPR (Q6LV37) DnaJ-like protein djlA 30 4.3
RIP3_RAT (Q9ERE6) Rho-interacting protein 3 (p116RIP) (RIP3) 29 5.6
RIP3_MOUSE (P97434) Rho-interacting protein 3 (p116RIP) (RIP3) 29 5.6
>EVPL_HUMAN (Q92817) Envoplakin (210 kDa paraneoplastic pemphigus
antigen) (p210) (210 kDa cornified envelope precursor)
Length = 2033
Score = 33.1 bits (74), Expect = 0.39
Identities = 27/97 (27%), Positives = 40/97 (40%), Gaps = 22/97 (22%)
Query: 107 LSGHQEALKSEIDAWYVQIFCQEK----------------SKEELLKEIRKKKEPKHPPA 150
+ HQEALK E + CQE S + L ++ + K+ PA
Sbjct: 304 IQAHQEALKMEWQNFLNLCICQETQLQHVEDYRRFQEEADSVSQTLAKLNSNLDAKYSPA 363
Query: 151 PQGPQPSQMELLM---ASMQRLETTH---GEMREENR 181
P GP + ELL A +RL T G+++ +R
Sbjct: 364 PGGPPGAPTELLQQLEAEEKRLAVTERATGDLQRRSR 400
>INVO_HUMAN (P07476) Involucrin
Length = 585
Score = 32.7 bits (73), Expect = 0.51
Identities = 30/99 (30%), Positives = 44/99 (44%), Gaps = 7/99 (7%)
Query: 83 QRDAPVMILPSIITDLCKFHNVPILSGHQEALKSEIDAWYVQIFCQEKSKEELLKEIRKK 142
Q PV + P++ +L K P+ + HQE +K + CQ K EL E+ K
Sbjct: 4 QHTLPVTLSPALSQELLKTVPPPV-NTHQEQMKQPTP---LPPPCQ-KVPVELPVEVPSK 58
Query: 143 KEPKHPPAPQG--PQPSQMELLMASMQRLETTHGEMREE 179
+E KH A +G Q + + Q L+ H E EE
Sbjct: 59 QEEKHMTAVKGLPEQECEQQQKEPQEQELQQQHWEQHEE 97
>C190_DROME (Q9VJE5) Restin homolog (Cytoplasmic linker protein 190)
(Microtubule binding protein 190) (d-CLIP-190)
Length = 1690
Score = 31.6 bits (70), Expect = 1.1
Identities = 24/89 (26%), Positives = 44/89 (48%), Gaps = 12/89 (13%)
Query: 96 TDLCKFHNVPILSGHQEALKSEIDAWYVQIFCQEKSKEELLKEIRKKKEPKHPPAPQGPQ 155
T L KF + I +GH+E L S+ DAW ++ +EK +EL ++++ ++ + +G +
Sbjct: 991 TKLSKFSD-EIATGHKE-LTSKADAWSQEMLQKEKELQELRQQLQDSQDSQTKLKAEGER 1048
Query: 156 PS----------QMELLMASMQRLETTHG 174
Q E+ A + LE + G
Sbjct: 1049 KEKSFEESIKNLQEEVTKAKTENLELSTG 1077
>AXN2_RAT (O70240) Axin 2 (Axis inhibition protein 2) (Conductin)
(Axin-like protein) (Axil)
Length = 838
Score = 31.6 bits (70), Expect = 1.1
Identities = 22/79 (27%), Positives = 34/79 (42%)
Query: 87 PVMILPSIITDLCKFHNVPILSGHQEALKSEIDAWYVQIFCQEKSKEELLKEIRKKKEPK 146
P + LP+ + P L G + ++ W ++ Q KSK + IRK +
Sbjct: 584 PGLALPAREGGMSSAAGAPQLPGEEGDRSQDVWQWMLESERQSKSKPHSTQSIRKSYPLE 643
Query: 147 HPPAPQGPQPSQMELLMAS 165
AP G + S+ LL AS
Sbjct: 644 SARAPPGERVSRHHLLGAS 662
>YHA2_YEAST (P38753) Hypothetical 51.2 kDa protein in LAG1-RPL14B
intergenic region
Length = 452
Score = 31.2 bits (69), Expect = 1.5
Identities = 30/125 (24%), Positives = 57/125 (45%), Gaps = 33/125 (26%)
Query: 57 NLLYSILEGHSV---------DIAAVMSNA------------IADAIQRDAPVMILPSII 95
+LLY+++E HSV D+ +S++ + D I+R AP ++ P++
Sbjct: 91 SLLYALIESHSVHITLKKAVTDVVKQLSDSFKDDPSLRAMGDLYDKIKRKAPYLVQPNV- 149
Query: 96 TDLCKFHNVPILSGH------QEALKSEIDAWYVQIFCQEKSKEELLKEIRKKKEPKHPP 149
+ HN+ + + Q+ALK + + Q QE+ KE E+ +++ +H
Sbjct: 150 ---PEKHNMSTQADNSDDEELQKALKMSLFEYEKQKKLQEQEKES--AEVLPQQQQQHQQ 204
Query: 150 APQGP 154
Q P
Sbjct: 205 QNQAP 209
>Y983_AQUAE (O67109) Hypothetical protein AQ_983
Length = 292
Score = 31.2 bits (69), Expect = 1.5
Identities = 21/56 (37%), Positives = 30/56 (53%), Gaps = 7/56 (12%)
Query: 98 LCKFH-NVPILSGHQEALKSEIDAWYVQIF------CQEKSKEELLKEIRKKKEPK 146
L K H NVP+ S + E KSE+ +Y ++F QE + E L ++ K KE K
Sbjct: 52 LQKIHLNVPVFSRNVEEDKSELPEYYYELFEDLKLESQESQRREELLDLLKGKEFK 107
>NKX1_HUMAN (O60721) Sodium/potassium/calcium exchanger 1 precursor
(Na(+)/K(+)/Ca(2+)-exchange protein 1) (Retinal rod
Na-Ca+K exchanger)
Length = 1099
Score = 31.2 bits (69), Expect = 1.5
Identities = 30/145 (20%), Positives = 57/145 (38%), Gaps = 11/145 (7%)
Query: 42 IWPSLHVTSLPVTKENLLYSILEGHSVDIAAVMSNAIADAIQRDAPVMILPSIIT---DL 98
+W ++ PV K L D++ AIA +D + LPS++T
Sbjct: 608 VWVKEQLSRRPVAKVMALE--------DLSKPGDGAIAVDELQDNKKLKLPSLLTRGSSS 659
Query: 99 CKFHNVPILSGHQEALKSEIDAWYVQIFCQEKSKEELLKEIRKKKEPKHPPAPQGPQPSQ 158
HN I S + + +D +EK +E L + R + + K P+ +P++
Sbjct: 660 TSLHNSTIRSTIYQLMLHSLDPLREVRLAKEKEEESLNQGARAQPQAKAESKPEEEEPAK 719
Query: 159 MELLMASMQRLETTHGEMREENRAQ 183
+ + + + G+ +E Q
Sbjct: 720 LPAVTVTPAPVPDIKGDQKENPGGQ 744
>MENE_BACCR (Q816I1) O-succinylbenzoate--CoA ligase (EC 6.2.1.26)
(OSB-CoA synthetase) (O-succinylbenzoyl-CoA synthetase)
Length = 482
Score = 30.8 bits (68), Expect = 1.9
Identities = 18/74 (24%), Positives = 37/74 (49%), Gaps = 5/74 (6%)
Query: 11 GLNEEEELTGGIPRRHMTQLSQVWTSLLLRNIWPSLHVTSLPVTKENLLYSILEGHSVDI 70
GL +++ +P H+ LS LL++NI + + +P + ++ L+ V I
Sbjct: 177 GLRDDDCWLACMPMFHVGGLS-----LLMKNIMYGMRILLVPKYDADFIHKALQTRGVTI 231
Query: 71 AAVMSNAIADAIQR 84
+V+S + D ++R
Sbjct: 232 ISVVSKMLTDLLER 245
>MENE_BACAN (Q81K97) O-succinylbenzoate--CoA ligase (EC 6.2.1.26)
(OSB-CoA synthetase) (O-succinylbenzoyl-CoA synthetase)
Length = 481
Score = 30.8 bits (68), Expect = 1.9
Identities = 18/74 (24%), Positives = 37/74 (49%), Gaps = 5/74 (6%)
Query: 11 GLNEEEELTGGIPRRHMTQLSQVWTSLLLRNIWPSLHVTSLPVTKENLLYSILEGHSVDI 70
GL +++ +P H+ LS LL++NI + + +P + ++ L+ V I
Sbjct: 176 GLRDDDCWLACMPMFHVGGLS-----LLMKNIMYGMRILLVPKYDADFIHKALQTRGVTI 230
Query: 71 AAVMSNAIADAIQR 84
+V+S + D ++R
Sbjct: 231 ISVVSKMLTDLLER 244
>SSXT_MOUSE (Q62280) SSXT protein (SYT protein) (Synovial sarcoma
associated Ss18-alpha)
Length = 418
Score = 30.4 bits (67), Expect = 2.5
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Query: 145 PKHPPAPQGPQPSQMELLMASMQRLETTHGEMREENRA 182
P H P QGP PSQ+ + +SM + HG M N +
Sbjct: 130 PNHMPM-QGPGPSQLSMTNSSMNMPSSNHGSMGGYNHS 166
>INVO_PONPY (P14708) Involucrin
Length = 835
Score = 30.4 bits (67), Expect = 2.5
Identities = 29/101 (28%), Positives = 45/101 (43%), Gaps = 7/101 (6%)
Query: 83 QRDAPVMILPSIITDLCKFHNVPILSGHQEALKSEIDAWYVQIFCQEKSKEELLKEIRKK 142
Q PV + P++ +L K P+ + QE +K + CQ K EL E+ K
Sbjct: 4 QHTLPVTLSPALSQELLKTVPPPV-NTQQEQMKQPTP---LPPPCQ-KVPVELPVEVPSK 58
Query: 143 KEPKHPPAPQG--PQPSQMELLMASMQRLETTHGEMREENR 181
+E KH A +G Q + + Q L+ H E EE++
Sbjct: 59 QEEKHMTAVKGLPEQECEQQQQEPQEQELQQQHWEQHEEHQ 99
>ATF5_HUMAN (Q9Y2D1) Cyclic-AMP-dependent transcription factor ATF-5
(Activating transcription factor 5) (Transcription
factor ATFx)
Length = 282
Score = 30.4 bits (67), Expect = 2.5
Identities = 17/62 (27%), Positives = 33/62 (52%), Gaps = 5/62 (8%)
Query: 125 IFCQEKSKEELLKEIRKKKEPKHPPAPQGPQPSQMELLMASMQRLETTHGEMREENRAQY 184
I+C+ ++ +E + + P+ PP P PQPS+ +A TT G+ +++ R Q
Sbjct: 164 IYCRNEAGQEEVG-MPPLPPPQQPPPPSPPQPSR----LAPYPHPATTRGDRKQKKRDQN 218
Query: 185 RA 186
++
Sbjct: 219 KS 220
>MX1_RAT (P18588) Interferon-induced GTP-binding protein Mx1
Length = 652
Score = 30.0 bits (66), Expect = 3.3
Identities = 17/63 (26%), Positives = 31/63 (48%), Gaps = 8/63 (12%)
Query: 125 IFCQEKSKEELLKEIRKKKEPKHPPAPQGPQPSQMELLMASMQRLETTHGEMREENRAQY 184
++CQ+++ ++ L+EIR+K+ K + S + R E T EM + A Y
Sbjct: 524 VYCQDQAYKKALQEIREKEAEK--------EKSTFGAFQHNSPRKELTTTEMTQHLNAYY 575
Query: 185 RAC 187
+ C
Sbjct: 576 QEC 578
>K179_HUMAN (Q14684) Protein KIAA0179
Length = 758
Score = 30.0 bits (66), Expect = 3.3
Identities = 13/34 (38%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Query: 124 QIFC-QEKSKEELLKEIRKKKEPKHPPAPQGPQP 156
++FC +E+ E L++ R+KK+ KH P+ P P
Sbjct: 383 RVFCVEEEDSESSLQKRRRKKKKKHHLQPENPGP 416
>INVO_PANPA (P14591) Involucrin
Length = 560
Score = 30.0 bits (66), Expect = 3.3
Identities = 29/99 (29%), Positives = 43/99 (43%), Gaps = 7/99 (7%)
Query: 83 QRDAPVMILPSIITDLCKFHNVPILSGHQEALKSEIDAWYVQIFCQEKSKEELLKEIRKK 142
Q PV + P++ +L K P+ + QE +K + CQ K EL E+ K
Sbjct: 4 QHTLPVTLSPALSQELLKTVPPPV-NTQQEQMKQPTP---LPPPCQ-KMPVELPVEVPSK 58
Query: 143 KEPKHPPAPQG--PQPSQMELLMASMQRLETTHGEMREE 179
+E KH A +G Q + + Q L+ H E EE
Sbjct: 59 QEEKHMTAVKGLPEQECEQQQQEPQEQELQQQHWEQHEE 97
>SSXT_HUMAN (Q15532) SSXT protein (Synovial sarcoma, translocated to
X chromosome) (SYT protein)
Length = 418
Score = 29.6 bits (65), Expect = 4.3
Identities = 14/38 (36%), Positives = 21/38 (54%), Gaps = 1/38 (2%)
Query: 145 PKHPPAPQGPQPSQMELLMASMQRLETTHGEMREENRA 182
P H P QGP P+Q+ + +SM ++HG M N +
Sbjct: 130 PNHMPM-QGPGPNQLNMTNSSMNMPSSSHGSMGGYNHS 166
>IF2_AQUAE (O67825) Translation initiation factor IF-2
Length = 805
Score = 29.6 bits (65), Expect = 4.3
Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 3/60 (5%)
Query: 111 QEALKSEIDAWYVQIFCQEKSKEELLKEIRKKKEPKHPPAPQGPQPSQMELLMASMQRLE 170
+E K E+ V+ +EK E +++EI +KKE + + P+ S EL+ +++ E
Sbjct: 84 EEEKKEEV---IVEEVVEEKKPEVIVEEIEEKKEEEEKKEEEKPKKSVEELIKEILEKKE 140
>DJLA_PHOPR (Q6LV37) DnaJ-like protein djlA
Length = 277
Score = 29.6 bits (65), Expect = 4.3
Identities = 17/63 (26%), Positives = 30/63 (46%), Gaps = 2/63 (3%)
Query: 119 DAWYVQIFCQEKSKEELLKEIRKKKEPKHPP--APQGPQPSQMELLMASMQRLETTHGEM 176
DA+ V + + +E+ + RK+ HP A +G P ME+ Q L+ + +
Sbjct: 211 DAYEVLGVTESATSQEVKRAYRKQMNEHHPDKLAAKGLPPEMMEIANQKAQELQAAYDMI 270
Query: 177 REE 179
R+E
Sbjct: 271 RKE 273
>RIP3_RAT (Q9ERE6) Rho-interacting protein 3 (p116RIP) (RIP3)
Length = 1029
Score = 29.3 bits (64), Expect = 5.6
Identities = 15/51 (29%), Positives = 24/51 (46%), Gaps = 4/51 (7%)
Query: 115 KSEIDAWYVQIFCQEKSKEELLKEIRKKKEPKHPPAPQGPQPSQMELLMAS 165
K I W + ++ K+ +KKK PP PQ P P++M + +S
Sbjct: 135 KEIISGWLEMLMVYPRTN----KQNQKKKRKVEPPTPQEPGPAKMAVTSSS 181
>RIP3_MOUSE (P97434) Rho-interacting protein 3 (p116RIP) (RIP3)
Length = 1024
Score = 29.3 bits (64), Expect = 5.6
Identities = 15/51 (29%), Positives = 24/51 (46%), Gaps = 4/51 (7%)
Query: 115 KSEIDAWYVQIFCQEKSKEELLKEIRKKKEPKHPPAPQGPQPSQMELLMAS 165
K I W + ++ K+ +KKK PP PQ P P++M + +S
Sbjct: 135 KEIISGWLEMLMVYPRTN----KQNQKKKRKVEPPTPQEPGPAKMAVTSSS 181
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.132 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,644,208
Number of Sequences: 164201
Number of extensions: 828319
Number of successful extensions: 3918
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 3894
Number of HSP's gapped (non-prelim): 42
length of query: 188
length of database: 59,974,054
effective HSP length: 104
effective length of query: 84
effective length of database: 42,897,150
effective search space: 3603360600
effective search space used: 3603360600
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0043b.1