BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0041.8
(562 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RDH1_HUMAN (Q92781) 11-cis retinol dehydrogenase (EC 1.1.1.105) ... 41 0.009
ER25_CANAL (O59933) C-4 methyl sterol oxidase (EC 1.-.-.-) 39 0.028
ER25_YEAST (P53045) C-4 methyl sterol oxidase (EC 1.-.-.-) 39 0.047
RDH1_BOVIN (Q27979) 11-cis retinol dehydrogenase (EC 1.1.1.105) ... 38 0.061
ER31_SCHPO (O94457) Probable C-5 sterol desaturase 1 (EC 1.3.3.-... 37 0.10
ER32_SCHPO (O13666) Probable C-5 sterol desaturase 2 (EC 1.3.3.-... 37 0.14
CCSA_OENHO (Q9MTI2) Cytochrome c biogenesis protein ccsA 36 0.31
ER25_SCHPO (Q9UUH4) C-4 methyl sterol oxidase (EC 1.-.-.-) 35 0.68
SC52_ARATH (Q9M883) Putative delta-7-sterol-C5(6)-desaturase 2 (... 33 1.5
NUFM_CAEEL (Q18359) Putative NADH-ubiquinone oxidoreductase 17.3... 33 1.5
YR29_BURPS (Q93SM8) Hypothetical UPF0226 protein BPSL2729 32 3.4
YBJS_ECOLI (P75821) Hypothetical protein ybjS 32 3.4
ROH3_RAT (P55006) Retinol dehydrogenase type III (EC 1.1.1.105) ... 32 3.4
ROH2_RAT (P50170) Retinol dehydrogenase type II (EC 1.1.1.105) (... 32 3.4
ROH1_RAT (P50169) Retinol dehydrogenase type I (EC 1.1.1.105) (R... 32 3.4
L767_CAEEL (Q09517) Putative steroid dehydrogenase let-767 (EC 1... 32 3.4
ER25_MOUSE (Q9CRA4) C-4 methyl sterol oxidase (EC 1.-.-.-) 32 3.4
SC5D_HUMAN (O75845) Lathosterol oxidase (EC 1.3.3.2) (Lathostero... 32 4.4
Y432_LISMO (P25145) Hypothetical oxidoreductase Lmo0432 (EC 1.-.... 32 5.8
KRSC_CHICK (P04459) Scale keratin (S-ker) (sKer) 32 5.8
>RDH1_HUMAN (Q92781) 11-cis retinol dehydrogenase (EC 1.1.1.105)
(11-cis RDH)
Length = 318
Score = 40.8 bits (94), Expect = 0.009
Identities = 22/73 (30%), Positives = 36/73 (49%)
Query: 377 IPKDVEEVFLTGATSKLGRAIALYLCQKKVKVLMLTLSADRFKRIQKEAPQEYQSYLVQV 436
+P VF+TG S GR +AL L Q+ +VL L+ + +Q+ A + L+ +
Sbjct: 24 LPASNAFVFITGCDSGFGRLLALQLDQRGFRVLASCLTPSGAEDLQRVASSRLHTTLLDI 83
Query: 437 TKYQAAQHCKTWI 449
T Q+ Q W+
Sbjct: 84 TDPQSVQQAAKWV 96
>ER25_CANAL (O59933) C-4 methyl sterol oxidase (EC 1.-.-.-)
Length = 308
Score = 39.3 bits (90), Expect = 0.028
Identities = 20/82 (24%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
Query: 50 LPSWNVKGVIVALVLHVGISEPLYYWVHRKFHGDYLFTNYHSLHHSSPVPESLTVGHATI 109
+P + +++ + + + +YW HR H + H HH P L +A
Sbjct: 133 VPFPKITDMLIQWAVFFVLEDTWHYWFHRGLHYGVFYKYIHKQHHRYAAPFGLAAEYAHP 192
Query: 110 VEHAFISV-VIGIPILGASVMG 130
VE A + + +GIPI+ + G
Sbjct: 193 VEVALLGLGTVGIPIVWCLITG 214
>ER25_YEAST (P53045) C-4 methyl sterol oxidase (EC 1.-.-.-)
Length = 309
Score = 38.5 bits (88), Expect = 0.047
Identities = 32/135 (23%), Positives = 52/135 (37%), Gaps = 6/135 (4%)
Query: 50 LPSWNVKGVIVALVLHVGISEPLYYWVHRKFHGDYLFTNYHSLHHSSPVPESLTVGHATI 109
+P ++K + + + L + + +YW HR FH + H HH P L+ +A
Sbjct: 133 VPFPSLKTMALEIGLFFVLEDTWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHP 192
Query: 110 VEHAFISV-VIGIPILGASVMGYGSASLVYGYVLGFDFLRCLGHCNVEVVPHGLFKTFPF 168
E + +G+PIL G + ++ F H + P L K PF
Sbjct: 193 AETLSLGFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHSGYD-FPWSLNKIMPF 251
Query: 169 MKYLLYTPTYHSLHH 183
+H LHH
Sbjct: 252 WA----GAEHHDLHH 262
>RDH1_BOVIN (Q27979) 11-cis retinol dehydrogenase (EC 1.1.1.105)
(11-cis RDH) (P32)
Length = 318
Score = 38.1 bits (87), Expect = 0.061
Identities = 21/73 (28%), Positives = 36/73 (48%)
Query: 377 IPKDVEEVFLTGATSKLGRAIALYLCQKKVKVLMLTLSADRFKRIQKEAPQEYQSYLVQV 436
+P +F+TG S GR +AL L Q+ +VL L+ + +Q+ A + L+ V
Sbjct: 24 LPASDAFIFITGCDSGFGRLLALRLDQRGFRVLASCLTPSGAEDLQRVASSRLHTTLLDV 83
Query: 437 TKYQAAQHCKTWI 449
T Q+ + W+
Sbjct: 84 TDPQSIRQAVKWV 96
>ER31_SCHPO (O94457) Probable C-5 sterol desaturase 1 (EC 1.3.3.-)
(Sterol-C5-desaturase 1) (Ergosterol delta 5,6
desaturase 1)
Length = 300
Score = 37.4 bits (85), Expect = 0.10
Identities = 16/46 (34%), Positives = 24/46 (51%)
Query: 49 NLPSWNVKGVIVALVLHVGISEPLYYWVHRKFHGDYLFTNYHSLHH 94
N+ + +K + +L L V S+ YW HR H Y++ H LHH
Sbjct: 109 NISDYGLKYFLCSLPLFVMFSDFGIYWAHRFLHHRYVYPRLHKLHH 154
>ER32_SCHPO (O13666) Probable C-5 sterol desaturase 2 (EC 1.3.3.-)
(Sterol-C5-desaturase 2) (Ergosterol delta 5,6
desaturase 2)
Length = 329
Score = 37.0 bits (84), Expect = 0.14
Identities = 15/39 (38%), Positives = 22/39 (55%)
Query: 61 ALVLHVGISEPLYYWVHRKFHGDYLFTNYHSLHHSSPVP 99
++ L + S+ L YW+HR H +L+ H LHH VP
Sbjct: 154 SIALFLLFSDFLIYWIHRALHHRWLYAPLHKLHHKWIVP 192
>CCSA_OENHO (Q9MTI2) Cytochrome c biogenesis protein ccsA
Length = 319
Score = 35.8 bits (81), Expect = 0.31
Identities = 31/146 (21%), Positives = 60/146 (40%), Gaps = 14/146 (9%)
Query: 5 TRNRRILQQGVDFKQIDKEWDWDNFLILQTIIASMAYYMLPFLQNLPSWNVKGVIVALV- 63
++ + L+ F +I + N L+ + I+S YY +Q L W+ + + + +
Sbjct: 173 SKKKAFLKDSFSFVEIQYRNEPSNVLLSTSFISSKNYYRAQLIQQLDRWSSRIISLGFIF 232
Query: 64 LHVGISE----------PLYYWVHRKFHGDYLFTNYHSLHHSSPVPESLTVGHATIVEHA 113
L +GI + W ++ +T + H+ P +V A +
Sbjct: 233 LTIGILSGAVWANEAWGSYWNWDPKETWAFITWTMFAIYLHTRTNPNFQSVNSAIVAFLG 292
Query: 114 FISVVI---GIPILGASVMGYGSASL 136
FI + I G+ +LG + YGS +L
Sbjct: 293 FIIIWICYFGVNLLGIGLHSYGSFNL 318
>ER25_SCHPO (Q9UUH4) C-4 methyl sterol oxidase (EC 1.-.-.-)
Length = 300
Score = 34.7 bits (78), Expect = 0.68
Identities = 17/63 (26%), Positives = 29/63 (45%)
Query: 49 NLPSWNVKGVIVALVLHVGISEPLYYWVHRKFHGDYLFTNYHSLHHSSPVPESLTVGHAT 108
++P V +I + L + + +YW HR FH + H +HH P L+ +A
Sbjct: 126 SVPFPPVTKMIWQITLFFFLEDTWHYWAHRLFHYGIFYRFIHKVHHRYSAPFGLSAEYAH 185
Query: 109 IVE 111
+E
Sbjct: 186 PLE 188
>SC52_ARATH (Q9M883) Putative delta-7-sterol-C5(6)-desaturase 2 (EC
1.3.3.-) (Delta-7-C-5 sterol desaturase 2)
(Delta7-sterol-C5-desaturase 2) (Homolog of DWF7
protein)
Length = 279
Score = 33.5 bits (75), Expect = 1.5
Identities = 35/160 (21%), Positives = 67/160 (41%), Gaps = 18/160 (11%)
Query: 34 TIIASMAYYMLPF-----LQNLPSWNVKGVIVALVLHVGISEPLYYWVHRKFHG-DYLFT 87
T++ +++ YM+ L +N + + L++ + E + YWVH++ H +L+
Sbjct: 100 TLLPAVSEYMIEHGWTKCYSTLDHFNWFLCFLYIALYLVLVEFMIYWVHKELHDIKFLYK 159
Query: 88 NYHSLHHSSPVPESLTVGHATIVEHAFISVVIGIPILGASVMGYGSASLVYGYVLGFDFL 147
+ H+ HH +L+ A + H ++ IP V+ + L FL
Sbjct: 160 HLHATHHMYNKQNTLS-PFAGLAFHPLDGILQAIP----HVIALFIVPIHLITHLSLLFL 214
Query: 148 RCLGHCNVEVVPHGLFKTFPFMKYLLYTPTYHSLHHTDNK 187
+ ++ HG +P M YH++HHT K
Sbjct: 215 EGIWTASIHDCIHG--NIWPIM-----GAGYHTIHHTTYK 247
>NUFM_CAEEL (Q18359) Putative NADH-ubiquinone oxidoreductase 17.3
kDa subunit (EC 1.6.5.3) (EC 1.6.99.3) (Complex
I-17.3KD) (CI-17.3KD)
Length = 150
Score = 33.5 bits (75), Expect = 1.5
Identities = 22/82 (26%), Positives = 43/82 (51%), Gaps = 5/82 (6%)
Query: 350 LFVDKHPNLRVRVVHGNTLTAAVIIDEIPKDVEEVFLTGATSKLGRAIALYLCQKKVKVL 409
LFV++HP+ + VV+G L A +++IP+D T A K + +AL + +K L
Sbjct: 48 LFVNEHPHRALTVVYGRILRA---LEQIPRDAAYRKYTEAVVK--QRLALVQAENDIKKL 102
Query: 410 MLTLSADRFKRIQKEAPQEYQS 431
+ + + + ++A E ++
Sbjct: 103 EEKIGMGQIEEVIEQAEYELET 124
>YR29_BURPS (Q93SM8) Hypothetical UPF0226 protein BPSL2729
Length = 407
Score = 32.3 bits (72), Expect = 3.4
Identities = 22/92 (23%), Positives = 37/92 (39%), Gaps = 3/92 (3%)
Query: 110 VEHAFISVVIGIPILGASVMGYGSASLVYGYVLGFDFLRCLGHCNVEVVPHGL---FKTF 166
+ HA I V+G+ ++ + +GY A L+ L V+PHGL +
Sbjct: 176 ISHALIPAVLGMLVIALAALGYYLARLITPVPLVHGERMSYASVLTRVLPHGLGLALGSA 235
Query: 167 PFMKYLLYTPTYHSLHHTDNKDTNFCLFMPLF 198
F + Y++ H N + +F LF
Sbjct: 236 GFGSIATFITLYYAARHWPNAALSLTVFGTLF 267
>YBJS_ECOLI (P75821) Hypothetical protein ybjS
Length = 337
Score = 32.3 bits (72), Expect = 3.4
Identities = 19/59 (32%), Positives = 30/59 (50%)
Query: 383 EVFLTGATSKLGRAIALYLCQKKVKVLMLTLSADRFKRIQKEAPQEYQSYLVQVTKYQA 441
+V +TGATS LGR +LCQK + V + K ++K + + L ++ QA
Sbjct: 2 KVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMGAEFVPADLTELVSSQA 60
>ROH3_RAT (P55006) Retinol dehydrogenase type III (EC 1.1.1.105)
(RODH III)
Length = 317
Score = 32.3 bits (72), Expect = 3.4
Identities = 15/66 (22%), Positives = 34/66 (50%)
Query: 384 VFLTGATSKLGRAIALYLCQKKVKVLMLTLSADRFKRIQKEAPQEYQSYLVQVTKYQAAQ 443
VF+TG S G +A L ++ ++VL L+ ++++ + ++ ++ VTK ++
Sbjct: 32 VFITGCDSGFGNLLARQLDRRGMRVLAACLTEKGAEQLRSKTSDRLETVILDVTKTESIV 91
Query: 444 HCKTWI 449
W+
Sbjct: 92 AATQWV 97
>ROH2_RAT (P50170) Retinol dehydrogenase type II (EC 1.1.1.105)
(RODH II) (29 k-protein)
Length = 317
Score = 32.3 bits (72), Expect = 3.4
Identities = 15/66 (22%), Positives = 34/66 (50%)
Query: 384 VFLTGATSKLGRAIALYLCQKKVKVLMLTLSADRFKRIQKEAPQEYQSYLVQVTKYQAAQ 443
VF+TG S G +A L ++ ++VL L+ ++++ + ++ ++ VTK ++
Sbjct: 32 VFITGCDSGFGNLLARQLDRRGMRVLAACLTEKGAEQLRSKTSDRLETVILDVTKTESIV 91
Query: 444 HCKTWI 449
W+
Sbjct: 92 AATQWV 97
>ROH1_RAT (P50169) Retinol dehydrogenase type I (EC 1.1.1.105) (RODH
I)
Length = 317
Score = 32.3 bits (72), Expect = 3.4
Identities = 15/66 (22%), Positives = 34/66 (50%)
Query: 384 VFLTGATSKLGRAIALYLCQKKVKVLMLTLSADRFKRIQKEAPQEYQSYLVQVTKYQAAQ 443
VF+TG S G +A L ++ ++VL L+ ++++ + ++ ++ VTK ++
Sbjct: 32 VFITGCDSGFGNLLARQLDRRGMRVLAACLTEKGAEQLRSKTSDRLETVILDVTKTESIV 91
Query: 444 HCKTWI 449
W+
Sbjct: 92 AATQWV 97
>L767_CAEEL (Q09517) Putative steroid dehydrogenase let-767 (EC
1.1.1.-) (Lethal protein 767)
Length = 316
Score = 32.3 bits (72), Expect = 3.4
Identities = 15/54 (27%), Positives = 30/54 (54%)
Query: 386 LTGATSKLGRAIALYLCQKKVKVLMLTLSADRFKRIQKEAPQEYQSYLVQVTKY 439
+TGAT +G+A A L ++ VL+++ + + +KE ++Y S V+ +
Sbjct: 52 VTGATDGIGKAYAFELARRGFNVLLVSRTQSKLDETKKEILEKYSSIEVRTAAF 105
>ER25_MOUSE (Q9CRA4) C-4 methyl sterol oxidase (EC 1.-.-.-)
Length = 293
Score = 32.3 bits (72), Expect = 3.4
Identities = 43/200 (21%), Positives = 75/200 (37%), Gaps = 30/200 (15%)
Query: 28 NFLILQTIIASMAYYMLPFL------QNLPSWNVKGVIVALVLHVGISEPLYYWVHRKFH 81
N +Q + YY F + +P W + + + I + +Y++HR H
Sbjct: 104 NHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYL--TLARCLGCAVIEDTWHYFLHRLLH 161
Query: 82 GDYLFTNYHSLHHSSPVPESLTVGHATIVEHAFISVVIGIPILGASVMGYGSASLVYGYV 141
++ H +HH P + +A H ++++G V+ L++ +V
Sbjct: 162 HKRIYKYIHKVHHEFQAPFGIEAEYA----HPLETLILGTGFFIGIVLLCDHVILLWAWV 217
Query: 142 LGFDFLRCLGHCNVEV---VPHGLFKTFPFMKYLLYT-PTYHSLHHTD---NKDTNFCLF 194
+R L +V +P PF YT +H HH + N + F +
Sbjct: 218 T----IRLLETIDVHSGYDIPLNPLNLVPF-----YTGARHHDFHHMNFIGNYASTFTWW 268
Query: 195 MPLF--DALGHTLNTKSWEL 212
LF DA H KS +L
Sbjct: 269 DKLFGTDAQYHAYIEKSKKL 288
>SC5D_HUMAN (O75845) Lathosterol oxidase (EC 1.3.3.2) (Lathosterol
5-desaturase) (Delta-7-sterol 5-desaturase) (C-5 sterol
desaturase) (Sterol-C5-desaturase)
Length = 299
Score = 32.0 bits (71), Expect = 4.4
Identities = 10/42 (23%), Positives = 22/42 (51%)
Query: 58 VIVALVLHVGISEPLYYWVHRKFHGDYLFTNYHSLHHSSPVP 99
++V+++ + ++ YW+HR H ++ H HH +P
Sbjct: 119 LVVSIISFLFFTDMFIYWIHRGLHHRLVYKRLHKPHHIWKIP 160
>Y432_LISMO (P25145) Hypothetical oxidoreductase Lmo0432 (EC
1.-.-.-) (ORFA)
Length = 248
Score = 31.6 bits (70), Expect = 5.8
Identities = 16/40 (40%), Positives = 27/40 (67%), Gaps = 3/40 (7%)
Query: 384 VFLTGATSKLGRAIALYLCQKKVKVLMLTLSADRFKRIQK 423
+ +TGA+S +G+A AL L +K K L L+A R ++++K
Sbjct: 8 IIITGASSGIGKATALLLAEKGAK---LVLAARRVEKLEK 44
>KRSC_CHICK (P04459) Scale keratin (S-ker) (sKer)
Length = 154
Score = 31.6 bits (70), Expect = 5.8
Identities = 13/25 (52%), Positives = 17/25 (68%)
Query: 120 GIPILGASVMGYGSASLVYGYVLGF 144
G PI G S +GYG +SL YG + G+
Sbjct: 64 GAPIFGGSSLGYGGSSLGYGGLYGY 88
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.141 0.450
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,161,360
Number of Sequences: 164201
Number of extensions: 3023892
Number of successful extensions: 8967
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 8943
Number of HSP's gapped (non-prelim): 35
length of query: 562
length of database: 59,974,054
effective HSP length: 115
effective length of query: 447
effective length of database: 41,090,939
effective search space: 18367649733
effective search space used: 18367649733
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0041.8