BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0041.13
(130 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SCA3_MOUSE (O35609) Secretory carrier-associated membrane protei... 47 1e-05
SCA3_HUMAN (O14828) Secretory carrier-associated membrane protei... 45 3e-05
SCA1_MOUSE (Q8K021) Secretory carrier-associated membrane protei... 45 3e-05
SCA2_MOUSE (Q9ERN0) Secretory carrier-associated membrane protei... 45 4e-05
SCA1_RAT (P56603) Secretory carrier-associated membrane protein ... 44 6e-05
SCA1_PIG (O77735) Secretory carrier-associated membrane protein ... 44 8e-05
SCA2_HUMAN (O15127) Secretory carrier-associated membrane protei... 43 1e-04
SCA1_HUMAN (O15126) Secretory carrier-associated membrane protei... 42 2e-04
SWC1_YEAST (P31376) SWC1 protein 39 0.003
NCB1_RAT (Q63083) Nucleobindin 1 precursor (CALNUC) (Bone 63 kDa... 36 0.017
NCB1_MOUSE (Q02819) Nucleobindin 1 precursor (CALNUC) 36 0.017
NCB1_HUMAN (Q02818) Nucleobindin 1 precursor (CALNUC) 35 0.029
YCF1_OENHO (Q9MTH5) Hypothetical 287.9 kDa protein ycf1 34 0.049
Z183_HUMAN (O15541) Zinc finger protein 183 34 0.064
M3KA_HUMAN (Q02779) Mitogen-activated protein kinase kinase kina... 34 0.064
YMDA_BACSU (O31774) Hypothetical UPF0144 protein ymdA 33 0.084
YC86_STAAM (P67277) Hypothetical UPF0144 protein SAV1286 33 0.084
YB69_STAAW (P67279) Hypothetical UPF0144 protein MW1169 33 0.084
YB29_STAAN (P67278) Hypothetical UPF0144 protein SA1129 33 0.084
NEK2_HUMAN (P51955) Serine/threonine-protein kinase Nek2 (EC 2.7... 33 0.14
>SCA3_MOUSE (O35609) Secretory carrier-associated membrane protein 3
(Secretory carrier membrane protein 3)
Length = 349
Score = 46.6 bits (109), Expect = 1e-05
Identities = 33/113 (29%), Positives = 46/113 (40%), Gaps = 14/113 (12%)
Query: 15 SVPLATSKLKPLPPEPYDRGATIDIPLDTTKDIKAKEKELQAREAELKRREQELKRKE-- 72
S P S K P EP + G+ T A EL ++ EL R+ +EL R+E
Sbjct: 67 SAPSVQSSRKLSPTEPRNYGS-----YSTQASAAAATAELLKKQEELNRKAEELDRRERE 121
Query: 73 -DAIARAGIVIEEKNWPPF------FPIIHHEISKEIPLHLQRIQYVAFITWL 118
+A G + NWPP P +IS EIP Q+ + W+
Sbjct: 122 LQHVALGGAGTRQNNWPPLPSFCPVKPCFFQDISMEIPQEFQKTVSTMYYLWM 174
>SCA3_HUMAN (O14828) Secretory carrier-associated membrane protein 3
(Secretory carrier membrane protein 3)
Length = 347
Score = 45.1 bits (105), Expect = 3e-05
Identities = 32/113 (28%), Positives = 45/113 (39%), Gaps = 14/113 (12%)
Query: 15 SVPLATSKLKPLPPEPYDRGATIDIPLDTTKDIKAKEKELQAREAELKRREQELKRKEDA 74
S P K P EP + G+ T A EL ++ EL R+ +EL R+E
Sbjct: 65 SAPSLQPSRKLSPTEPKNYGS-----YSTQASAAAATAELLKKQEELNRKAEELDRRERE 119
Query: 75 IARA---GIVIEEKNWPPF------FPIIHHEISKEIPLHLQRIQYVAFITWL 118
+ A G + NWPP P +IS EIP Q+ + W+
Sbjct: 120 LQHAALGGTATRQNNWPPLPSFCPVQPCFFQDISMEIPQEFQKTVSTMYYLWM 172
>SCA1_MOUSE (Q8K021) Secretory carrier-associated membrane protein 1
(Secretory carrier membrane protein 1)
Length = 338
Score = 45.1 bits (105), Expect = 3e-05
Identities = 35/131 (26%), Positives = 60/131 (45%), Gaps = 9/131 (6%)
Query: 6 EMEPARNSGSVPLATSKLKPLP-PEPYDRGATIDIPLDT--TKDIKAKEKELQAREAELK 62
E P +S + P + K+ +P +P T + P T TK+ + EL R+ EL+
Sbjct: 36 EYNPFSDSRTPPPGSVKMPNVPNTQPAIMKPTEEHPAYTQITKEHALAQAELLKRQEELE 95
Query: 63 RREQELKRKEDAIARAGIVIEEKNWPPF---FPI---IHHEISKEIPLHLQRIQYVAFIT 116
R+ EL R+E + + NWPP FP+ + + S +IP+ Q+ + +
Sbjct: 96 RKAAELDRREREMQNLSQHGRKNNWPPLPSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYL 155
Query: 117 WLGMLKFLFFS 127
W+ LF +
Sbjct: 156 WMFHAVTLFLN 166
>SCA2_MOUSE (Q9ERN0) Secretory carrier-associated membrane protein 2
(Secretory carrier membrane protein 2)
Length = 329
Score = 44.7 bits (104), Expect = 4e-05
Identities = 30/128 (23%), Positives = 56/128 (43%), Gaps = 19/128 (14%)
Query: 5 SEMEPARNSGSVPLATSKLKPLPPEPYDRGATIDIPLDTTKDIKA--------KEKELQA 56
+E P + + + P P +P +++ T + + A +++EL
Sbjct: 35 AEFNPFSETNAATTVPATQAPGPSQPAVLQPSVEPAQPTPQAVAAAAQAGLLRQQEELDR 94
Query: 57 REAELKRREQELKRKEDAIARAGIVIEEKNWPPF------FPIIHHEISKEIPLHLQRIQ 110
+ AEL+R+E+EL+ A + + + NWPP P + + S EIP QRI
Sbjct: 95 KAAELERKERELQN-----TAANLHVRDNNWPPLPSWCPVKPCFYQDFSTEIPADYQRIC 149
Query: 111 YVAFITWL 118
+ + W+
Sbjct: 150 KMLYYLWM 157
>SCA1_RAT (P56603) Secretory carrier-associated membrane protein 1
(Secretory carrier membrane protein 1) (SCAMP 37)
Length = 338
Score = 43.9 bits (102), Expect = 6e-05
Identities = 35/131 (26%), Positives = 59/131 (44%), Gaps = 9/131 (6%)
Query: 6 EMEPARNSGSVPLATSKLKPLP-PEPYDRGATIDIPLDT--TKDIKAKEKELQAREAELK 62
E P +S + P K+ +P +P T + P T TK+ + EL R+ EL+
Sbjct: 36 EYNPFSDSRTPPPGGVKMPNVPNTQPAIMKPTEEHPAYTQITKEHALAQAELLKRQEELE 95
Query: 63 RREQELKRKEDAIARAGIVIEEKNWPPF---FPI---IHHEISKEIPLHLQRIQYVAFIT 116
R+ EL R+E + + NWPP FP+ + + S +IP+ Q+ + +
Sbjct: 96 RKAAELDRREREMQNLSQHGRKNNWPPLPSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYL 155
Query: 117 WLGMLKFLFFS 127
W+ LF +
Sbjct: 156 WMFHAVTLFLN 166
>SCA1_PIG (O77735) Secretory carrier-associated membrane protein 1
(Secretory carrier membrane protein 1)
Length = 338
Score = 43.5 bits (101), Expect = 8e-05
Identities = 34/131 (25%), Positives = 58/131 (43%), Gaps = 9/131 (6%)
Query: 6 EMEPARNSGSVPLATSKLKPLPP-EPYDRGATIDIPLDT--TKDIKAKEKELQAREAELK 62
E P +S + P K+ +P +P T + P T K+ + EL R+ EL+
Sbjct: 36 EYNPFSDSRTPPPGNVKMPNVPSTQPAIMKPTEEHPAYTQIAKEHALAQAELLKRQEELE 95
Query: 63 RREQELKRKEDAIARAGIVIEEKNWPPF---FPI---IHHEISKEIPLHLQRIQYVAFIT 116
R+ EL R+E + + NWPP FP+ + + S +IP+ Q+ + +
Sbjct: 96 RKAAELDRREREMQNLSQHGRKNNWPPLPGNFPVGPCFYQDFSVDIPVEFQKTVKIMYYL 155
Query: 117 WLGMLKFLFFS 127
W+ LF +
Sbjct: 156 WMFHAVTLFLN 166
>SCA2_HUMAN (O15127) Secretory carrier-associated membrane protein 2
(Secretory carrier membrane protein 2)
Length = 329
Score = 42.7 bits (99), Expect = 1e-04
Identities = 23/75 (30%), Positives = 39/75 (51%), Gaps = 11/75 (14%)
Query: 50 KEKELQAREAELKRREQELKRKEDAIARAGIVIEEKNWPPF------FPIIHHEISKEIP 103
+++EL + AEL+R+E+EL+ A + + + NWPP P + + S EIP
Sbjct: 88 QQEELDRKAAELERKERELQNTV-----ANLHVRQNNWPPLPSWCPVKPCFYQDFSTEIP 142
Query: 104 LHLQRIQYVAFITWL 118
QRI + + W+
Sbjct: 143 ADYQRICKMLYYLWM 157
>SCA1_HUMAN (O15126) Secretory carrier-associated membrane protein 1
(Secretory carrier membrane protein 1)
Length = 338
Score = 42.0 bits (97), Expect = 2e-04
Identities = 34/131 (25%), Positives = 58/131 (43%), Gaps = 9/131 (6%)
Query: 6 EMEPARNSGSVPLATSKLKPLP-PEPYDRGATIDIPLDT--TKDIKAKEKELQAREAELK 62
E P +S + P K+ +P +P T + P T K+ + EL R+ EL+
Sbjct: 36 EYNPFSDSRTPPPGGVKMPNVPNTQPAIMKPTEEHPAYTQIAKEHALAQAELLKRQEELE 95
Query: 63 RREQELKRKEDAIARAGIVIEEKNWPPF---FPI---IHHEISKEIPLHLQRIQYVAFIT 116
R+ EL R+E + + NWPP FP+ + + S +IP+ Q+ + +
Sbjct: 96 RKAAELDRREREMQNLSQHGRKNNWPPLPSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYL 155
Query: 117 WLGMLKFLFFS 127
W+ LF +
Sbjct: 156 WMFHAVTLFLN 166
>SWC1_YEAST (P31376) SWC1 protein
Length = 625
Score = 38.5 bits (88), Expect = 0.003
Identities = 36/109 (33%), Positives = 54/109 (49%), Gaps = 12/109 (11%)
Query: 5 SEMEPARN----SGSVPLATSKLKPLPPEPYDRGA-TIDIPLDTTKDIKAKEKELQAREA 59
SE PA SG++PL K++ + G T + L K+ K ++K E
Sbjct: 130 SESTPAATESPTSGTIPLIRDKMQKMCDCVMSGGPHTFKVRLFILKNDKIEQKWQD--EQ 187
Query: 60 ELKRREQELKRKEDAIARAGIVIEEKNWPPFFPIIHHEISKEIPLHLQR 108
ELK++E+ELKRK DA A+ + E K + +I+KE L LQ+
Sbjct: 188 ELKKKEKELKRKNDAEAKRLRMEERKRQQ-----MQKKIAKEQKLQLQK 231
>NCB1_RAT (Q63083) Nucleobindin 1 precursor (CALNUC) (Bone 63 kDa
calcium-binding protein)
Length = 459
Score = 35.8 bits (81), Expect = 0.017
Identities = 17/39 (43%), Positives = 26/39 (66%)
Query: 40 PLDTTKDIKAKEKELQAREAELKRREQELKRKEDAIARA 78
P T +++K E+EL AREAEL R Q L ++ +A+ R+
Sbjct: 339 PAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRS 377
>NCB1_MOUSE (Q02819) Nucleobindin 1 precursor (CALNUC)
Length = 459
Score = 35.8 bits (81), Expect = 0.017
Identities = 17/39 (43%), Positives = 26/39 (66%)
Query: 40 PLDTTKDIKAKEKELQAREAELKRREQELKRKEDAIARA 78
P T +++K E+EL AREAEL R Q L ++ +A+ R+
Sbjct: 339 PAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRS 377
>NCB1_HUMAN (Q02818) Nucleobindin 1 precursor (CALNUC)
Length = 461
Score = 35.0 bits (79), Expect = 0.029
Identities = 19/62 (30%), Positives = 35/62 (55%)
Query: 40 PLDTTKDIKAKEKELQAREAELKRREQELKRKEDAIARAGIVIEEKNWPPFFPIIHHEIS 99
P T ++++ E+EL AREAEL + Q L ++ +A+ R+ +E + ++H E
Sbjct: 340 PAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQQ 399
Query: 100 KE 101
K+
Sbjct: 400 KQ 401
>YCF1_OENHO (Q9MTH5) Hypothetical 287.9 kDa protein ycf1
Length = 2434
Score = 34.3 bits (77), Expect = 0.049
Identities = 13/33 (39%), Positives = 26/33 (78%)
Query: 45 KDIKAKEKELQAREAELKRREQELKRKEDAIAR 77
K++K+K+K +++++ E+KR+ E+KRK + I R
Sbjct: 791 KNVKSKQKNVKSKQNEIKRKVNEIKRKVNEIKR 823
Score = 32.0 bits (71), Expect = 0.24
Identities = 13/41 (31%), Positives = 28/41 (67%)
Query: 45 KDIKAKEKELQAREAELKRREQELKRKEDAIARAGIVIEEK 85
K++K+K+K +++++ +K ++ E+KRK + I R I+ K
Sbjct: 784 KNVKSKQKNVKSKQKNVKSKQNEIKRKVNEIKRKVNEIKRK 824
Score = 31.6 bits (70), Expect = 0.32
Identities = 12/36 (33%), Positives = 25/36 (69%)
Query: 45 KDIKAKEKELQAREAELKRREQELKRKEDAIARAGI 80
K++K+K+ E++ + E+KR+ E+KRK++ G+
Sbjct: 798 KNVKSKQNEIKRKVNEIKRKVNEIKRKQNESYPRGV 833
>Z183_HUMAN (O15541) Zinc finger protein 183
Length = 343
Score = 33.9 bits (76), Expect = 0.064
Identities = 30/84 (35%), Positives = 41/84 (48%), Gaps = 8/84 (9%)
Query: 6 EMEPARNSGSVPLATSKLKPLPPEPYDRGATIDIPLDTTKDIKAKEKELQAREAELKRRE 65
E EP + G V +T KP+ PE D GAT LDT KE++ QA ++ +
Sbjct: 90 ENEP-ESLGVVYKSTRSAKPVGPE--DMGATAVYELDT-----EKERDAQAIFERSQKIQ 141
Query: 66 QELKRKEDAIARAGIVIEEKNWPP 89
+EL+ KED GI +K P
Sbjct: 142 EELRGKEDDKIYRGINNYQKYMKP 165
>M3KA_HUMAN (Q02779) Mitogen-activated protein kinase kinase kinase
10 (EC 2.7.1.37) (Mixed lineage kinase 2) (Protein
kinase MST)
Length = 954
Score = 33.9 bits (76), Expect = 0.064
Identities = 18/47 (38%), Positives = 30/47 (63%), Gaps = 7/47 (14%)
Query: 46 DIKAKEKELQAREAELKR-------REQELKRKEDAIARAGIVIEEK 85
D++ KEKEL++RE EL R +E++L+R+E +A + I E+
Sbjct: 390 DLRTKEKELRSREEELLRAAQEQRFQEEQLRRREQELAEREMDIVER 436
Score = 26.9 bits (58), Expect = 7.9
Identities = 14/51 (27%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Query: 29 EPYDRGATIDIPLDTTKDIKAKEK-ELQAREAELKRREQELKRKEDAIARA 78
E ++ A +PL++ ++ K E+Q +L+ +E+EL+ +E+ + RA
Sbjct: 358 EVIEQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRA 408
>YMDA_BACSU (O31774) Hypothetical UPF0144 protein ymdA
Length = 520
Score = 33.5 bits (75), Expect = 0.084
Identities = 13/46 (28%), Positives = 29/46 (62%)
Query: 41 LDTTKDIKAKEKELQAREAELKRREQELKRKEDAIARAGIVIEEKN 86
+D ++++ + ELQ +E L ++E+ L RK + I + ++E+K+
Sbjct: 71 IDAEQEVRERRNELQKQENRLLQKEENLDRKHEGIDKREAMLEKKD 116
>YC86_STAAM (P67277) Hypothetical UPF0144 protein SAV1286
Length = 519
Score = 33.5 bits (75), Expect = 0.084
Identities = 13/43 (30%), Positives = 27/43 (62%)
Query: 43 TTKDIKAKEKELQAREAELKRREQELKRKEDAIARAGIVIEEK 85
T +++ + ELQ +E L ++E+ L+RK D + + ++E+K
Sbjct: 72 TEAELRERRSELQRQETRLLQKEENLERKSDLLDKKDEILEQK 114
Score = 30.0 bits (66), Expect = 0.93
Identities = 13/36 (36%), Positives = 25/36 (69%)
Query: 51 EKELQAREAELKRREQELKRKEDAIARAGIVIEEKN 86
E EL+ R +EL+R+E L +KE+ + R ++++K+
Sbjct: 73 EAELRERRSELQRQETRLLQKEENLERKSDLLDKKD 108
>YB69_STAAW (P67279) Hypothetical UPF0144 protein MW1169
Length = 519
Score = 33.5 bits (75), Expect = 0.084
Identities = 13/43 (30%), Positives = 27/43 (62%)
Query: 43 TTKDIKAKEKELQAREAELKRREQELKRKEDAIARAGIVIEEK 85
T +++ + ELQ +E L ++E+ L+RK D + + ++E+K
Sbjct: 72 TEAELRERRSELQRQETRLLQKEENLERKSDLLDKKDEILEQK 114
Score = 30.0 bits (66), Expect = 0.93
Identities = 13/36 (36%), Positives = 25/36 (69%)
Query: 51 EKELQAREAELKRREQELKRKEDAIARAGIVIEEKN 86
E EL+ R +EL+R+E L +KE+ + R ++++K+
Sbjct: 73 EAELRERRSELQRQETRLLQKEENLERKSDLLDKKD 108
>YB29_STAAN (P67278) Hypothetical UPF0144 protein SA1129
Length = 519
Score = 33.5 bits (75), Expect = 0.084
Identities = 13/43 (30%), Positives = 27/43 (62%)
Query: 43 TTKDIKAKEKELQAREAELKRREQELKRKEDAIARAGIVIEEK 85
T +++ + ELQ +E L ++E+ L+RK D + + ++E+K
Sbjct: 72 TEAELRERRSELQRQETRLLQKEENLERKSDLLDKKDEILEQK 114
Score = 30.0 bits (66), Expect = 0.93
Identities = 13/36 (36%), Positives = 25/36 (69%)
Query: 51 EKELQAREAELKRREQELKRKEDAIARAGIVIEEKN 86
E EL+ R +EL+R+E L +KE+ + R ++++K+
Sbjct: 73 EAELRERRSELQRQETRLLQKEENLERKSDLLDKKD 108
>NEK2_HUMAN (P51955) Serine/threonine-protein kinase Nek2 (EC
2.7.1.37) (NimA-related protein kinase 2) (NimA-like
protein kinase 1) (HSPK 21)
Length = 445
Score = 32.7 bits (73), Expect = 0.14
Identities = 16/40 (40%), Positives = 25/40 (62%)
Query: 46 DIKAKEKELQAREAELKRREQELKRKEDAIARAGIVIEEK 85
++K KE +LQ RE LK RE+ L++KE + + E+K
Sbjct: 305 ELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDK 344
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.138 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,833,952
Number of Sequences: 164201
Number of extensions: 660862
Number of successful extensions: 4924
Number of sequences better than 10.0: 210
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 80
Number of HSP's that attempted gapping in prelim test: 4448
Number of HSP's gapped (non-prelim): 474
length of query: 130
length of database: 59,974,054
effective HSP length: 106
effective length of query: 24
effective length of database: 42,568,748
effective search space: 1021649952
effective search space used: 1021649952
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0041.13