BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0036c.6
(70 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KIWI_ARATH (O65154) RNA polymerase II transcriptional coactivato... 112 1e-25
KELP_ARATH (O65155) RNA polymerase II transcriptional coactivato... 71 6e-13
TCP4_MOUSE (P11031) Activated RNA polymerase II transcriptional ... 66 1e-11
TCP4_RAT (Q63396) Activated RNA polymerase II transcriptional co... 63 1e-10
TCP4_HUMAN (P53999) Activated RNA polymerase II transcriptional ... 63 2e-10
TCP4_DROME (Q9VLR5) Putative RNA polymerase II transcriptional c... 58 5e-09
TCP4_SCHPO (P87294) Putative RNA polymerase II transcriptional c... 55 3e-08
SUB1_YEAST (P54000) RNA polymerase II transcriptional coactivato... 55 4e-08
TCP4_NEUCR (Q872F4) Putative RNA polymerase II transcriptional c... 51 5e-07
TCP4_CAEEL (Q94045) Putative RNA polymerase II transcriptional c... 50 1e-06
KDKA_PASPI (P58551) 3-deoxy-D-manno-octulosonic acid kinase (EC ... 28 3.3
FPG_VIBVY (Q7MPS3) Formamidopyrimidine-DNA glycosylase (EC 3.2.2... 28 3.3
FPG_VIBVU (Q8DDY4) Formamidopyrimidine-DNA glycosylase (EC 3.2.2... 28 3.3
SYFB_CLOTE (Q891T8) Phenylalanyl-tRNA synthetase beta chain (EC ... 27 7.4
GAA1_YEAST (P39012) GPI transamidase component GAA1 27 7.4
G6PD_PSEAE (O68282) Glucose-6-phosphate 1-dehydrogenase (EC 1.1.... 27 9.7
>KIWI_ARATH (O65154) RNA polymerase II transcriptional coactivator
KIWI
Length = 107
Score = 112 bits (281), Expect = 1e-25
Identities = 50/67 (74%), Positives = 60/67 (88%)
Query: 3 DSIVVCEISKNRRVSVRNWQGRIVVDIREFYVKDGKQMPGKKGISLTMDQWNVLRNHIEE 62
D IVVC ISKNRRVSVRNW G+I +DIREFYVKDGK +PGKKGISL++DQWN LRNH E+
Sbjct: 39 DDIVVCNISKNRRVSVRNWNGKIWIDIREFYVKDGKTLPGKKGISLSVDQWNTLRNHAED 98
Query: 63 IDKAVNE 69
I+KA+++
Sbjct: 99 IEKALSD 105
>KELP_ARATH (O65155) RNA polymerase II transcriptional coactivator
KELP
Length = 165
Score = 70.9 bits (172), Expect = 6e-13
Identities = 28/69 (40%), Positives = 49/69 (70%)
Query: 1 DPDSIVVCEISKNRRVSVRNWQGRIVVDIREFYVKDGKQMPGKKGISLTMDQWNVLRNHI 60
D +++C +S RRV+++ ++G+ +V IRE+Y KDGK++P KGISLT +QW+ + ++
Sbjct: 92 DDGDLIICRLSDKRRVTIQEFKGKSLVSIREYYKKDGKELPTSKGISLTDEQWSTFKKNM 151
Query: 61 EEIDKAVNE 69
I+ AV +
Sbjct: 152 PAIENAVKK 160
>TCP4_MOUSE (P11031) Activated RNA polymerase II transcriptional
coactivator p15 precursor (Positive cofactor 4) (PC4)
(p14) (Single-stranded DNA binding protein p9)
Length = 126
Score = 66.2 bits (160), Expect = 1e-11
Identities = 30/62 (48%), Positives = 48/62 (77%), Gaps = 1/62 (1%)
Query: 9 EISKNRRVSVRNWQGRIVVDIREFYV-KDGKQMPGKKGISLTMDQWNVLRNHIEEIDKAV 67
+I K R VSVR+++G+I++DIRE+++ +G+ PG+KGISL M+QW+ L+ I +ID AV
Sbjct: 64 QIGKMRYVSVRDFKGKILIDIREYWMDSEGEMKPGRKGISLNMEQWSQLKEQISDIDDAV 123
Query: 68 NE 69
+
Sbjct: 124 RK 125
>TCP4_RAT (Q63396) Activated RNA polymerase II transcriptional
coactivator p15 (Positive cofactor 4) (PC4) (p14)
(Fragment)
Length = 119
Score = 63.2 bits (152), Expect = 1e-10
Identities = 29/62 (46%), Positives = 47/62 (75%), Gaps = 1/62 (1%)
Query: 9 EISKNRRVSVRNWQGRIVVDIREFYV-KDGKQMPGKKGISLTMDQWNVLRNHIEEIDKAV 67
+I K R VSVR+++G+I++DIRE+++ +G+ P +KGISL M+QW+ L+ I +ID AV
Sbjct: 57 QIGKMRYVSVRDFKGKILIDIREYWMDSEGEMKPRRKGISLNMEQWSQLKEQISDIDDAV 116
Query: 68 NE 69
+
Sbjct: 117 RK 118
>TCP4_HUMAN (P53999) Activated RNA polymerase II transcriptional
coactivator p15 (Positive cofactor 4) (PC4) (p14)
Length = 126
Score = 62.8 bits (151), Expect = 2e-10
Identities = 28/62 (45%), Positives = 47/62 (75%), Gaps = 1/62 (1%)
Query: 9 EISKNRRVSVRNWQGRIVVDIREFYVK-DGKQMPGKKGISLTMDQWNVLRNHIEEIDKAV 67
+I K R VSVR+++G++++DIRE+++ +G+ PG+KGISL +QW+ L+ I +ID AV
Sbjct: 64 QIGKMRYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDIDDAV 123
Query: 68 NE 69
+
Sbjct: 124 RK 125
>TCP4_DROME (Q9VLR5) Putative RNA polymerase II transcriptional
coactivator
Length = 110
Score = 57.8 bits (138), Expect = 5e-09
Identities = 27/54 (50%), Positives = 38/54 (70%)
Query: 14 RRVSVRNWQGRIVVDIREFYVKDGKQMPGKKGISLTMDQWNVLRNHIEEIDKAV 67
R+V + ++GR VDIREFY K G+ +PGKKGISL++ QW L EE+ +A+
Sbjct: 55 RQVRINEFRGRKSVDIREFYDKGGQILPGKKGISLSLIQWKKLLEVAEEVTRAI 108
>TCP4_SCHPO (P87294) Putative RNA polymerase II transcriptional
coactivator
Length = 136
Score = 55.5 bits (132), Expect = 3e-08
Identities = 22/57 (38%), Positives = 40/57 (69%)
Query: 11 SKNRRVSVRNWQGRIVVDIREFYVKDGKQMPGKKGISLTMDQWNVLRNHIEEIDKAV 67
++ +R+++ ++G V IRE+Y KDG +PGKKGI+L +++W L+ I E+D ++
Sbjct: 35 TEKKRITLSEFRGTRYVHIREYYEKDGDMLPGKKGIALNINEWKKLKQLIHEVDDSL 91
>SUB1_YEAST (P54000) RNA polymerase II transcriptional coactivator
SUB1
Length = 292
Score = 54.7 bits (130), Expect = 4e-08
Identities = 28/66 (42%), Positives = 44/66 (66%), Gaps = 2/66 (3%)
Query: 4 SIVVCEISKNRRVSVRNWQGRIVVDIREFYVKD--GKQMPGKKGISLTMDQWNVLRNHIE 61
S + ++ KN+RV+VR ++ ++DIRE+Y+ G+ PGKKGISLT D ++ L H
Sbjct: 37 SDAIFDLGKNKRVTVRQFRNINLIDIREYYLDSSTGEMKPGKKGISLTEDLYDELLKHRL 96
Query: 62 EIDKAV 67
ID+A+
Sbjct: 97 NIDEAL 102
>TCP4_NEUCR (Q872F4) Putative RNA polymerase II transcriptional
coactivator
Length = 172
Score = 51.2 bits (121), Expect = 5e-07
Identities = 26/64 (40%), Positives = 39/64 (60%)
Query: 1 DPDSIVVCEISKNRRVSVRNWQGRIVVDIREFYVKDGKQMPGKKGISLTMDQWNVLRNHI 60
D D E+ NRR+S ++ +V+IRE+Y GK MPGKKGISL++ Q+ L I
Sbjct: 39 DQDGNTFWELGNNRRISSSVFRNTTLVNIREYYDAGGKLMPGKKGISLSLAQYQNLLKVI 98
Query: 61 EEID 64
+++
Sbjct: 99 PQLN 102
>TCP4_CAEEL (Q94045) Putative RNA polymerase II transcriptional
coactivator
Length = 124
Score = 50.1 bits (118), Expect = 1e-06
Identities = 28/67 (41%), Positives = 40/67 (58%), Gaps = 2/67 (2%)
Query: 1 DPDSIVVCEISKNRRVSVRNWQGRIVVDIREFYVK--DGKQMPGKKGISLTMDQWNVLRN 58
D D + EI R +V ++G+ V+IRE+Y+ K MP +KGISL+ QW L++
Sbjct: 56 DSDGNEMFEIGNLRYATVSKFKGKEYVNIREYYIDRDSQKMMPSRKGISLSKAQWANLKD 115
Query: 59 HIEEIDK 65
I EIDK
Sbjct: 116 LIPEIDK 122
>KDKA_PASPI (P58551) 3-deoxy-D-manno-octulosonic acid kinase (EC
2.7.1.-) (KDO kinase)
Length = 240
Score = 28.5 bits (62), Expect = 3.3
Identities = 14/56 (25%), Positives = 26/56 (46%), Gaps = 4/56 (7%)
Query: 1 DPDSIVVCEISKNRRVSVRNWQGRIVVDIREFYVKDGKQMPGKKGISLTMDQWNVL 56
D ++ + + K R+ NWQ + + + ++K+ GK+GI D W L
Sbjct: 177 DHKTVWIIDFDKCYRLEGANWQEKNLARLHRSFIKE----QGKRGILFNEDNWQWL 228
>FPG_VIBVY (Q7MPS3) Formamidopyrimidine-DNA glycosylase (EC
3.2.2.23) (Fapy-DNA glycosylase)
Length = 269
Score = 28.5 bits (62), Expect = 3.3
Identities = 16/66 (24%), Positives = 36/66 (54%), Gaps = 6/66 (9%)
Query: 3 DSIVVCEISKNRRVSVRNW--QGRIVVDIREFYVKDG----KQMPGKKGISLTMDQWNVL 56
D + E ++ +RV+V+ + +IVV + Y + + +P + +SL+ ++W L
Sbjct: 139 DGQYMFEKAQGKRVAVKQFIMDNKIVVGVGNIYASESLFRSRILPTRATMSLSAEEWQRL 198
Query: 57 RNHIEE 62
+HI++
Sbjct: 199 VSHIKQ 204
>FPG_VIBVU (Q8DDY4) Formamidopyrimidine-DNA glycosylase (EC
3.2.2.23) (Fapy-DNA glycosylase)
Length = 269
Score = 28.5 bits (62), Expect = 3.3
Identities = 16/66 (24%), Positives = 36/66 (54%), Gaps = 6/66 (9%)
Query: 3 DSIVVCEISKNRRVSVRNW--QGRIVVDIREFYVKDG----KQMPGKKGISLTMDQWNVL 56
D + E ++ +RV+V+ + +IVV + Y + + +P + +SL+ ++W L
Sbjct: 139 DGQYMFEKAQGKRVAVKQFIMDNKIVVGVGNIYASESLFRSRILPTRATMSLSAEEWQRL 198
Query: 57 RNHIEE 62
+HI++
Sbjct: 199 VSHIKQ 204
>SYFB_CLOTE (Q891T8) Phenylalanyl-tRNA synthetase beta chain (EC
6.1.1.20) (Phenylalanine--tRNA ligase beta chain)
(PheRS)
Length = 791
Score = 27.3 bits (59), Expect = 7.4
Identities = 13/34 (38%), Positives = 23/34 (67%), Gaps = 1/34 (2%)
Query: 18 VRNWQGRIVVDIREFYVKDGKQMP-GKKGISLTM 50
+RN G+I+ D++ F V G Q+P GKK ++ ++
Sbjct: 724 IRNKGGKILEDVKLFDVYKGAQIPEGKKSVAYSI 757
>GAA1_YEAST (P39012) GPI transamidase component GAA1
Length = 614
Score = 27.3 bits (59), Expect = 7.4
Identities = 11/39 (28%), Positives = 19/39 (48%)
Query: 30 REFYVKDGKQMPGKKGISLTMDQWNVLRNHIEEIDKAVN 68
R Y+ + MP + +WN+LR + +I + VN
Sbjct: 49 RRTYISENALMPSQAYSYFRESEWNILRGYRSQIKEMVN 87
>G6PD_PSEAE (O68282) Glucose-6-phosphate 1-dehydrogenase (EC
1.1.1.49) (G6PD)
Length = 489
Score = 26.9 bits (58), Expect = 9.7
Identities = 13/29 (44%), Positives = 18/29 (61%), Gaps = 4/29 (13%)
Query: 15 RVSVRNWQGRIVVDIREFYVKDGKQMPGK 43
RV +RNW+ V FY++ GK+MP K
Sbjct: 318 RVDIRNWRWSGV----PFYLRTGKRMPQK 342
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,174,615
Number of Sequences: 164201
Number of extensions: 256022
Number of successful extensions: 648
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 632
Number of HSP's gapped (non-prelim): 16
length of query: 70
length of database: 59,974,054
effective HSP length: 46
effective length of query: 24
effective length of database: 52,420,808
effective search space: 1258099392
effective search space used: 1258099392
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0036c.6