BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0035.10
         (150 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YHP7_YEAST (P38809) Hypothetical 40.7 kDa protein in HXT5-NRK1 i...    32  0.68
MYOC_RAT (Q9R1J4) Myocilin precursor (Trabecular meshwork-induce...    30  2.6
VNSC_RINDR (Q03339) Nonstructural protein C                            28  5.8
VNSC_RINDK (P35948) Nonstructural protein C                            28  5.8
TRMB_CAEEL (Q23126) Probable tRNA (guanine-N(7)-)-methyltransfer...    28  7.5
NIFN_HERSE (O87627) Nitrogenase iron-molybdenum cofactor biosynt...    28  7.5
IFP1_CAEEL (Q09501) Intermediate filament protein ifp-1 (Interme...    28  7.5
RECO_THETN (Q8RB48) DNA repair protein recO (Recombination prote...    28  9.8
CAL0_HORSE (Q9N0V5) Calcitonin precursor                               28  9.8

>YHP7_YEAST (P38809) Hypothetical 40.7 kDa protein in HXT5-NRK1
           intergenic region
          Length = 366

 Score = 31.6 bits (70), Expect = 0.68
 Identities = 15/57 (26%), Positives = 25/57 (43%), Gaps = 5/57 (8%)

Query: 35  KKNTTQHNDRDSSELRDQVSIHPSSS-----SFIITMLLGPSLHQCGWMDGCMPTRN 86
           ++ +  H D+D  + + +  + P  +        +T L G S H  G  D C P RN
Sbjct: 104 RRTSHGHRDKDKQKSKSRTKVKPPKNVDTIDKMDVTGLFGGSFHHDGPFDACTPQRN 160


>MYOC_RAT (Q9R1J4) Myocilin precursor (Trabecular meshwork-induced
           glucocorticoid response protein)
          Length = 502

 Score = 29.6 bits (65), Expect = 2.6
 Identities = 20/58 (34%), Positives = 24/58 (40%), Gaps = 2/58 (3%)

Query: 70  PSLHQCGWMDGCMPTRNSYDTSGSLSHADTRATSAKATCLLSWFLLHQSLSRSLTYPQ 127
           PS   C   D  M          S+ HAD  +T A+   L S  LLHQ  S  +T  Q
Sbjct: 55  PSESSCPREDQAMSAIQDLQRDSSIQHADLESTKARVRSLES--LLHQMTSGGVTGTQ 110


>VNSC_RINDR (Q03339) Nonstructural protein C
          Length = 177

 Score = 28.5 bits (62), Expect = 5.8
 Identities = 19/55 (34%), Positives = 22/55 (39%), Gaps = 7/55 (12%)

Query: 81  CMPTRNSYDTSGSLSHADTRATSAKATCLLSWF-------LLHQSLSRSLTYPQC 128
           C PTR       S SHA  +   AKA CL            + +S   SL  PQC
Sbjct: 41  CPPTRPKQTIRISASHASQQLDQAKAACLAVTIKDLEEATAVMRSWEHSLVTPQC 95


>VNSC_RINDK (P35948) Nonstructural protein C
          Length = 177

 Score = 28.5 bits (62), Expect = 5.8
 Identities = 19/55 (34%), Positives = 22/55 (39%), Gaps = 7/55 (12%)

Query: 81  CMPTRNSYDTSGSLSHADTRATSAKATCLLSWF-------LLHQSLSRSLTYPQC 128
           C PTR       S SHA  +   AKA CL            + +S   SL  PQC
Sbjct: 41  CPPTRPKQTIRISASHASQQLDQAKAACLAVTIRDLEEATAVMRSWEHSLVTPQC 95


>TRMB_CAEEL (Q23126) Probable tRNA
          (guanine-N(7)-)-methyltransferase (EC 2.1.1.33)
          (tRNA(m7G46)-methyltransferase)
          Length = 256

 Score = 28.1 bits (61), Expect = 7.5
 Identities = 12/42 (28%), Positives = 25/42 (58%), Gaps = 1/42 (2%)

Query: 4  LVPSLTRMKSGNHRPCCSYITLHMQRKLKKKKKNTTQHNDRD 45
          + P+LT M+  +H+P C  +    Q+K  +++ ++  H+D D
Sbjct: 3  ITPALTEMEL-DHKPTCETVPGLPQKKHYRQRAHSNPHSDHD 43


>NIFN_HERSE (O87627) Nitrogenase iron-molybdenum cofactor
           biosynthesis protein nifN
          Length = 443

 Score = 28.1 bits (61), Expect = 7.5
 Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 8/65 (12%)

Query: 45  DSSELRDQVSIHPSSSSFIITMLLGPSLHQCGWMDGCMPTRNSYDTSGSLSHADTRATSA 104
           D  E+RD V       SF +  ++ P +    W+DG +P   S  + G  + A+ RA  A
Sbjct: 178 DIEEMRDIVQ------SFGLEPIVLPDVSS--WLDGHLPDNFSPTSMGGTTLAEMRALGA 229

Query: 105 KATCL 109
              C+
Sbjct: 230 SIVCI 234


>IFP1_CAEEL (Q09501) Intermediate filament protein ifp-1
           (Intermediate filament protein E1) (IF-E1) (Cel IF E1)
          Length = 776

 Score = 28.1 bits (61), Expect = 7.5
 Identities = 28/101 (27%), Positives = 43/101 (41%), Gaps = 5/101 (4%)

Query: 6   PSLTRMKSGNHRPCCSYITLHMQRKLKKKKKNTTQHNDRDSSELRDQVSIHPSSSSFII- 64
           PS   + S   R   S I+ H  R      ++T  H  RDSS  R   +IH + +   I 
Sbjct: 375 PSTQVIASALSRQNYSSISDHHSRTSSGIVQHTAHHAVRDSSSSRTGTTIHETITHTPIP 434

Query: 65  ---TMLLGPSLHQCGWMDGCMPTRNSYDTSGSLSHADTRAT 102
              T+ + P + +  +     P R SY      S AD+R++
Sbjct: 435 IPATLPVQP-IREYTYSRDASPIRPSYTPYQQESRADSRSS 474


>RECO_THETN (Q8RB48) DNA repair protein recO (Recombination protein
           O)
          Length = 247

 Score = 27.7 bits (60), Expect = 9.8
 Identities = 24/82 (29%), Positives = 38/82 (46%), Gaps = 8/82 (9%)

Query: 2   WRLVPSLTRMKSGNHR-PCCSYITLHMQRKLKKKKKNT-----TQHNDRDSSELRDQVSI 55
           W LV +   ++    +    SYI+  + R L++K+KNT     T+H+      L  + SI
Sbjct: 73  WELVKNFENIEKDLKKFALASYISETISRVLEEKQKNTKLYFFTKHSLEAVESLNVETSI 132

Query: 56  HPSSSSFIITMLLG--PSLHQC 75
              S +  +  LLG  P L  C
Sbjct: 133 FLFSYTLKLISLLGYMPVLDSC 154


>CAL0_HORSE (Q9N0V5) Calcitonin precursor
          Length = 140

 Score = 27.7 bits (60), Expect = 9.8
 Identities = 22/64 (34%), Positives = 32/64 (49%), Gaps = 5/64 (7%)

Query: 89  DTSG-SLSHADTRATSAKATCLLSWFLLHQSLSRSLTYPQCK--VQTPKKYQVVAVGLEM 145
           +T G SL     +  S  +TC+L  +   Q L++  T+PQ    V  P K +V+A GLE 
Sbjct: 70  ETGGASLDSPRAKRCSNLSTCVLGTYT--QDLNKFHTFPQTAIGVGAPGKKRVMARGLER 127

Query: 146 GSAP 149
              P
Sbjct: 128 DHGP 131


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.318    0.128    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,119,244
Number of Sequences: 164201
Number of extensions: 593927
Number of successful extensions: 1342
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 1342
Number of HSP's gapped (non-prelim): 9
length of query: 150
length of database: 59,974,054
effective HSP length: 100
effective length of query: 50
effective length of database: 43,553,954
effective search space: 2177697700
effective search space used: 2177697700
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)