BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0032.10
         (216 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

HYPD_ALCEU (P31903) Hydrogenase expression/formation protein hypD      33  0.66
RIF1_YEAST (P29539) RIF1 protein (RAP1-interacting factor 1)           32  1.1
THAB_PAROL (Q91242) Thyroid hormone receptor alpha B (THR-alpha-B)     32  1.5
RPC2_SOYBN (Q8HVY3) DNA-directed RNA polymerase beta'' chain (EC...    32  1.5
RPC2_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'' chain (EC...    31  1.9
RPC2_CALFE (Q7YJY0) DNA-directed RNA polymerase beta'' chain (EC...    30  5.6
METN_YERPE (Q8ZH38) D-methionine transport ATP-binding protein metN    30  5.6
ACKA_PASMU (P57866) Acetate kinase (EC 2.7.2.1) (Acetokinase)          30  5.6
VRPR_SALTY (P13041) Virulence genes transcriptional activator          29  9.5
VRPR_SALDU (P24417) Virulence genes transcriptional activator          29  9.5

>HYPD_ALCEU (P31903) Hydrogenase expression/formation protein hypD
          Length = 379

 Score = 32.7 bits (73), Expect = 0.66
 Identities = 30/104 (28%), Positives = 45/104 (42%), Gaps = 15/104 (14%)

Query: 116 PQVIIVCDSLDVIRLIIDPVKFVKSEKALYEKNFARTV--------KVRPKRTWKVRPSR 167
           P VI   + LDV++ I+  V+ V S +A  E  F R V        +      +++RPS 
Sbjct: 223 PVVIAGFEPLDVMQAILMLVRQVNSGRAEVENEFVRAVTRDGNESAQAMVSEVFELRPSF 282

Query: 168 TWKNLQIPTPYSSRFTIRSGAVWKIGQFF------IPDAKSTNC 205
            W+ L    PYS+       A +   Q F      +PD K+  C
Sbjct: 283 EWRGLG-EVPYSALRIRAQFARFDAEQRFDLRYRPVPDNKACEC 325


>RIF1_YEAST (P29539) RIF1 protein (RAP1-interacting factor 1)
          Length = 1916

 Score = 32.0 bits (71), Expect = 1.1
 Identities = 39/187 (20%), Positives = 75/187 (39%), Gaps = 36/187 (19%)

Query: 24  DSEALSEYDGILEAASGFIKSLGMEQHVDVYVARLASVIGIYMRVVGVLRDAMGQWIKGF 83
           D+E++SE+  I+E   G ++     Q  + YVAR   V   +  ++ +L           
Sbjct: 206 DNESVSEFKEIIEGGLGILR-----QESEDYVARRFEVYATFNNIIPIL----------- 249

Query: 84  SINMGSRAYANYFLAQFTTVSTAVEIVIGLEIPQVIIVCDSL---------DVIRLIIDP 134
                +    N    +F  +   +E +I + IP + I  D+L          VIRL +  
Sbjct: 250 -----TTKNVNEVDQKFNILIVNIESIIEICIPHLQIAQDTLLSSSEKKNPFVIRLYVQI 304

Query: 135 VKFVKSEKALYEKNFARTVKVRPKRTWKVRPSRTWKNLQIPTPYSSRFTIRSGAVW---- 190
           V+F  +  + ++    + +  RP    K++    W    +    S++  I +   +    
Sbjct: 305 VRFFSAIMSNFK--IVKWLTKRPDLVNKLKVIYRWTTGALRNENSNKIIITAQVSFLRDE 362

Query: 191 KIGQFFI 197
           K G FF+
Sbjct: 363 KFGTFFL 369


>THAB_PAROL (Q91242) Thyroid hormone receptor alpha B (THR-alpha-B)
          Length = 391

 Score = 31.6 bits (70), Expect = 1.5
 Identities = 24/73 (32%), Positives = 39/73 (52%), Gaps = 5/73 (6%)

Query: 130 LIIDPVKFVKSEKALYEKNFARTVKVRPKRTWKVRPSRT---WKNLQIPTPYSSRFTIRS 186
           L++D  K V +++ L E+N  R  K    +T + RP  T   W+ +++ T  + R T   
Sbjct: 104 LVLDDSKRV-AKRRLIEENRERRKKEEIVKTLQNRPEPTGAEWELIRMVTE-AHRHTNAQ 161

Query: 187 GAVWKIGQFFIPD 199
           GA WK  + F+PD
Sbjct: 162 GAQWKQKRKFLPD 174


>RPC2_SOYBN (Q8HVY3) DNA-directed RNA polymerase beta'' chain (EC
            2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
            subunit) (RNA polymerase beta'' subunit)
          Length = 1384

 Score = 31.6 bits (70), Expect = 1.5
 Identities = 14/55 (25%), Positives = 32/55 (57%)

Query: 10   LVKSYMCELRHTGDDSEALSEYDGILEAASGFIKSLGMEQHVDVYVARLASVIGI 64
            ++ +++ E   +GD ++ L + + +LE  S    S+ +E+ VD +  R+  ++GI
Sbjct: 1133 ILVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISMNLEKRVDTWNGRITKILGI 1187


>RPC2_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'' chain (EC
            2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
            subunit) (RNA polymerase beta'' subunit)
          Length = 1332

 Score = 31.2 bits (69), Expect = 1.9
 Identities = 14/58 (24%), Positives = 33/58 (56%)

Query: 10   LVKSYMCELRHTGDDSEALSEYDGILEAASGFIKSLGMEQHVDVYVARLASVIGIYMR 67
            ++ +++ E   +GD ++ L + + +LE  S    S+ +E+ +D +  R+  ++GI  R
Sbjct: 1105 ILVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISMNLEKRIDAWNERITGILGIPWR 1162


>RPC2_CALFE (Q7YJY0) DNA-directed RNA polymerase beta'' chain (EC
            2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
            subunit) (RNA polymerase beta'' subunit)
          Length = 1377

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 14/52 (26%), Positives = 31/52 (58%)

Query: 13   SYMCELRHTGDDSEALSEYDGILEAASGFIKSLGMEQHVDVYVARLASVIGI 64
            +++ E   +GD ++ L + + +LEA S    S+ +E+ V+ +  R+  ++GI
Sbjct: 1129 TFIYEKSRSGDITQGLPKVEQVLEARSIDSISMNLEKRVEGWNERITKILGI 1180


>METN_YERPE (Q8ZH38) D-methionine transport ATP-binding protein metN
          Length = 343

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 18/66 (27%), Positives = 33/66 (49%), Gaps = 1/66 (1%)

Query: 118 VIIVCDSLDVIRLIIDPVKFVKSEKALYEKNFARTVKVRPKRTWKVRPSRTWKNLQIPTP 177
           ++++   +DV++ I D V  + SE  L EK+    V   PK     +  ++  +L IP  
Sbjct: 194 ILLITHEMDVVKRICDQVAVI-SEGKLIEKDSVSEVFSHPKTPLAQQFIQSTLHLDIPED 252

Query: 178 YSSRFT 183
           Y+ R +
Sbjct: 253 YAKRMS 258


>ACKA_PASMU (P57866) Acetate kinase (EC 2.7.2.1) (Acetokinase)
          Length = 401

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 15/40 (37%), Positives = 22/40 (54%)

Query: 37  AASGFIKSLGMEQHVDVYVARLASVIGIYMRVVGVLRDAM 76
           A   + K    ++ +DV+  RLA  IG YM V+G   DA+
Sbjct: 289 AEDNYDKEASAKRALDVFSYRLAKYIGSYMAVIGERLDAI 328


>VRPR_SALTY (P13041) Virulence genes transcriptional activator
          Length = 297

 Score = 28.9 bits (63), Expect = 9.5
 Identities = 17/49 (34%), Positives = 24/49 (48%)

Query: 94  NYFLAQFTTVSTAVEIVIGLEIPQVIIVCDSLDVIRLIIDPVKFVKSEK 142
           NYF  +     T+VE  + L IP+   +C   D+ RL   PV F +  K
Sbjct: 149 NYFHRESLVCRTSVEGGVMLFIPKKFFLCGKPDINRLAGTPVLFHEGAK 197


>VRPR_SALDU (P24417) Virulence genes transcriptional activator
          Length = 297

 Score = 28.9 bits (63), Expect = 9.5
 Identities = 17/49 (34%), Positives = 24/49 (48%)

Query: 94  NYFLAQFTTVSTAVEIVIGLEIPQVIIVCDSLDVIRLIIDPVKFVKSEK 142
           NYF  +     T+VE  + L IP+   +C   D+ RL   PV F +  K
Sbjct: 149 NYFHRESLVCRTSVEGGVMLFIPKKFFLCGKPDINRLAGTPVLFHEGAK 197


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.322    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,746,846
Number of Sequences: 164201
Number of extensions: 902597
Number of successful extensions: 1989
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1983
Number of HSP's gapped (non-prelim): 10
length of query: 216
length of database: 59,974,054
effective HSP length: 106
effective length of query: 110
effective length of database: 42,568,748
effective search space: 4682562280
effective search space used: 4682562280
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)