BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0031.15
(168 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
FAS1_YEAST (P07149) Fatty acid synthase subunit beta (EC 2.3.1.8... 42 9e-04
DHB4_RAT (P97852) Peroxisomal multifunctional enzyme type 2 (MFE... 40 0.003
Y736_TROW8 (Q83HA5) Hypothetical UPF0336 protein TW736 39 0.007
Y721_TROWT (Q83FK2) Hypothetical UPF0336 protein TWT721 39 0.007
MAOC_ECOLI (P77455) MaoC protein (Phenylacetic acid degradation ... 39 0.007
FOX2_YEAST (Q02207) Peroxisomal hydratase-dehydrogenase-epimeras... 39 0.007
DHB4_MOUSE (P51660) Peroxisomal multifunctional enzyme type 2 (M... 38 0.012
FOX2_NEUCR (Q01373) Peroxisomal hydratase-dehydrogenase-epimeras... 36 0.047
DHB4_HUMAN (P51659) Peroxisomal multifunctional enzyme type 2 (M... 35 0.11
TOXC_COCCA (Q92215) Putative fatty acid synthase subunit TOXC [I... 33 0.31
FOX2_CANTR (P22414) Peroxisomal hydratase-dehydrogenase-epimeras... 33 0.31
MAOC_KLEAE (P49251) MAOC protein 33 0.40
FAS1_YARLI (P34229) Fatty acid synthase subunit beta (EC 2.3.1.8... 32 0.52
FAPR_THETN (Q8R9V5) Transcription factor fapR (Fatty acid and ph... 32 0.52
NODN_RHIME (P25200) Nodulation protein N 31 1.2
FAS1_CANAL (P34731) Fatty acid synthase subunit beta (EC 2.3.1.8... 31 1.2
NEK4_MOUSE (Q9Z1J2) Serine/threonine-protein kinase Nek4 (EC 2.7... 31 1.5
FXE1_HUMAN (O00358) Forkhead box protein E1 (Thyroid transcripti... 29 4.4
FABZ_BRUSU (Q8G0E4) (3R)-hydroxymyristoyl-[acyl carrier protein]... 29 4.4
TRYP_SQUAC (P00764) Trypsin precursor (EC 3.4.21.4) 29 5.8
>FAS1_YEAST (P07149) Fatty acid synthase subunit beta (EC 2.3.1.86)
[Includes: 3-hydroxypalmitoyl-[acyl-carrier-protein]
dehydratase (EC 4.2.1.61); Enoyl-[acyl-carrier-protein]
reductase [NADH] (EC 1.3.1.9); [Acyl-carrier-protein]
acetyltransferase (EC 2.
Length = 2051
Score = 41.6 bits (96), Expect = 9e-04
Identities = 33/118 (27%), Positives = 50/118 (41%), Gaps = 13/118 (11%)
Query: 48 YSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPHIISSHFPGAV------YVSQS 101
Y++V+ DLNP+H A G + HGM ++ +I + +V Y Q
Sbjct: 1553 YARVSGDLNPIHVSRHFASYANLPGTITHGMFSSASVRALIENWAADSVSSRVRGYTCQF 1612
Query: 102 LNFKFPVYIGDQVIGEVQATNLRANKNRYLVKFKTRCIKSDELVAIEGEAVAMLPTLT 159
++ P I V N R L+KF+TR D++V + GEA P T
Sbjct: 1613 VDMVLPNTALKTSIQHVGMIN-----GRKLIKFETR--NEDDVVVLTGEAEIEQPVTT 1663
>DHB4_RAT (P97852) Peroxisomal multifunctional enzyme type 2 (MFE-2)
(D-bifunctional protein) (DBP) (17-beta-hydroxysteroid
dehydrogenase 4) (17-beta-HSD 4) [Includes:
D-3-hydroxyacyl-CoA dehydratase (EC 4.2.1.-);
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1
Length = 735
Score = 40.0 bits (92), Expect = 0.003
Identities = 35/139 (25%), Positives = 64/139 (45%), Gaps = 13/139 (9%)
Query: 22 AATPQVLKPGDVLRKARAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVA 81
AA +P D + + + L+ +++ D NPLH D + A GFE P++HG+
Sbjct: 479 AAVAVPSRPPDAVLRDTTSLNQAALY--RLSGDSNPLHIDPSFASIAGFEKPILHGLCTF 536
Query: 82 SLFP-HIISSHFPGAVYVSQSLNFKF--PVYIGDQVIGEVQATNLRANKNRYLVKFKTRC 138
H++ V +++ +F PVY G+ T + NR + F+T+
Sbjct: 537 GFSARHVLQQFADNDVSRFKAIKVRFAKPVY-----PGQTLQTEMWKEGNR--IHFQTKV 589
Query: 139 IKSDELVAIEGEAVAMLPT 157
++ ++V I V ++PT
Sbjct: 590 QETGDIV-ISNAYVDLVPT 607
>Y736_TROW8 (Q83HA5) Hypothetical UPF0336 protein TW736
Length = 140
Score = 38.5 bits (88), Expect = 0.007
Identities = 23/83 (27%), Positives = 41/83 (48%), Gaps = 8/83 (9%)
Query: 38 RAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEG----PLVHGMLVASLFPHIISS--- 90
R TEE ++ +++ H N ++ +AAA + G+ G P + V + +IISS
Sbjct: 10 RHVTEEKLMEFARAVHTQNKIYHSTAAATSSGYPGLVAVPTFGAVWVGQILEYIISSDLN 69
Query: 91 -HFPGAVYVSQSLNFKFPVYIGD 112
+ V+ Q +K P++ GD
Sbjct: 70 IDYSHIVHGEQHFLYKRPIFAGD 92
>Y721_TROWT (Q83FK2) Hypothetical UPF0336 protein TWT721
Length = 140
Score = 38.5 bits (88), Expect = 0.007
Identities = 23/83 (27%), Positives = 41/83 (48%), Gaps = 8/83 (9%)
Query: 38 RAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEG----PLVHGMLVASLFPHIISS--- 90
R TEE ++ +++ H N ++ +AAA + G+ G P + V + +IISS
Sbjct: 10 RHVTEEKLMEFARAVHTQNKIYHSTAAATSSGYPGLVAVPTFGAVWVGQILEYIISSDLN 69
Query: 91 -HFPGAVYVSQSLNFKFPVYIGD 112
+ V+ Q +K P++ GD
Sbjct: 70 IDYSHIVHGEQHFLYKRPIFAGD 92
>MAOC_ECOLI (P77455) MaoC protein (Phenylacetic acid degradation
protein paaZ)
Length = 681
Score = 38.5 bits (88), Expect = 0.007
Identities = 30/91 (32%), Positives = 43/91 (46%), Gaps = 4/91 (4%)
Query: 28 LKPGD-VLRKARAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPH 86
L+PGD +L R TE D+++++ ++ D H D AA F +VHG V S
Sbjct: 534 LQPGDSLLTPRRTMTEADIVNFACLSGDHFYAHMDKIAAAESIFGERVVHGYFVLSAAAG 593
Query: 87 IISSHFPGAV---YVSQSLNFKFPVYIGDQV 114
+ G V Y +SL F PV GD +
Sbjct: 594 LFVDAGVGPVIANYGLESLRFIEPVKPGDTI 624
>FOX2_YEAST (Q02207) Peroxisomal hydratase-dehydrogenase-epimerase
(HDE) (Multifunctional beta-oxidation protein) (MFP)
[Includes: 2-enoyl-CoA hydratase (EC 4.2.1.-);
D-3-hydroxyacyl CoA dehydrogenase (EC 1.1.1.-)]
Length = 900
Score = 38.5 bits (88), Expect = 0.007
Identities = 26/101 (25%), Positives = 45/101 (43%), Gaps = 9/101 (8%)
Query: 36 KARAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPHIISSHFPGA 95
+A T +D +++ D NPLH D A+ V F P++HG+ + + H+
Sbjct: 785 EAEISTNKDQAALYRLSGDFNPLHIDPTLAKAVKFPTPILHGLCTLGISAKALFEHY--G 842
Query: 96 VYVSQSLNFKFPVYIGDQVIGEVQATNLRANKNRYLVKFKT 136
Y + F V+ GD + ++A K +V F+T
Sbjct: 843 PYEELKVRFTNVVFPGDTL-------KVKAWKQGSVVVFQT 876
>DHB4_MOUSE (P51660) Peroxisomal multifunctional enzyme type 2
(MFE-2) (D-bifunctional protein) (DBP)
(17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4)
[Includes: D-3-hydroxyacyl-CoA dehydratase (EC 4.2.1.-);
3-hydroxyacyl-CoA dehydrogenase (EC 1.1
Length = 735
Score = 37.7 bits (86), Expect = 0.012
Identities = 34/130 (26%), Positives = 60/130 (46%), Gaps = 14/130 (10%)
Query: 30 PGDVLRKARAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFP-HII 88
P VLR A + + + +++ D NPLH D A GFE P++HG+ H++
Sbjct: 488 PDAVLRDATSLNQAALY---RLSGDWNPLHIDPDFASVAGFEKPILHGLCTFGFSARHVL 544
Query: 89 SSHFPGAVYVSQSLNFKF--PVYIGDQVIGEVQATNLRANKNRYLVKFKTRCIKSDELVA 146
V +++ +F PVY G+ T + NR + F+T+ ++ ++V
Sbjct: 545 QQFADNDVSRFKAIKVRFAKPVY-----PGQTLQTEMWKEGNR--IHFQTKVHETGDVV- 596
Query: 147 IEGEAVAMLP 156
I V ++P
Sbjct: 597 ISNAYVDLVP 606
>FOX2_NEUCR (Q01373) Peroxisomal hydratase-dehydrogenase-epimerase
(HDE) (Multifunctional beta-oxidation protein) (MFP)
[Includes: 2-enoyl-CoA hydratase (EC 4.2.1.-);
D-3-hydroxyacyl CoA dehydrogenase (EC 1.1.1.-)]
Length = 894
Score = 35.8 bits (81), Expect = 0.047
Identities = 21/59 (35%), Positives = 34/59 (57%), Gaps = 2/59 (3%)
Query: 20 SSAATPQVLKPGDVLRKARAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGM 78
S+AA + D + + + TEE Y +++ D NPLH D A A+ GF+ P++HG+
Sbjct: 771 STAANKPPARSPDAVVEVQT-TEEQAAIY-RLSGDYNPLHVDPAFAKVGGFKVPILHGL 827
>DHB4_HUMAN (P51659) Peroxisomal multifunctional enzyme type 2
(MFE-2) (D-bifunctional protein) (DBP)
(17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4)
[Includes: D-3-hydroxyacyl-CoA dehydratase (EC 4.2.1.-);
3-hydroxyacyl-CoA dehydrogenase (EC 1.1
Length = 736
Score = 34.7 bits (78), Expect = 0.11
Identities = 28/111 (25%), Positives = 49/111 (43%), Gaps = 11/111 (9%)
Query: 50 KVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPHIISSHFPG---AVYVSQSLNFKF 106
+++ D NPLH D A GF+ P++HG+ + F + + + F
Sbjct: 506 RLSGDWNPLHIDPNFASLAGFDKPILHGLCTFGFSARRVLQQFADNDVSRFKAIKARFAK 565
Query: 107 PVYIGDQVIGEVQATNLRANKNRYLVKFKTRCIKSDELVAIEGEAVAMLPT 157
PVY G+ T + NR + F+T+ ++ ++V I V + PT
Sbjct: 566 PVY-----PGQTLQTEMWKEGNR--IHFQTKVQETGDIV-ISNAYVDLAPT 608
>TOXC_COCCA (Q92215) Putative fatty acid synthase subunit TOXC
[Includes: 3-hydroxypalmitoyl-[acyl-carrier-protein]
dehydratase (EC 4.2.1.61); Enoyl-[acyl-carrier-protein]
reductase [NADH] (EC 1.3.1.9); [Acyl-carrier-protein]
acetyltransferase (EC 2.3.1.3
Length = 2080
Score = 33.1 bits (74), Expect = 0.31
Identities = 12/41 (29%), Positives = 22/41 (53%)
Query: 48 YSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPHII 88
Y++ + DLNP+H A+ G G + HGM ++ ++
Sbjct: 1571 YARTSGDLNPIHVSRTFAEYAGLPGLITHGMYCSAAIQDLV 1611
>FOX2_CANTR (P22414) Peroxisomal hydratase-dehydrogenase-epimerase
(HDE) (Multifunctional beta-oxidation protein) (MFP)
[Includes: 2-enoyl-CoA hydratase (EC 4.2.1.-);
D-3-hydroxyacyl CoA dehydrogenase (EC 1.1.1.-)]
Length = 906
Score = 33.1 bits (74), Expect = 0.31
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 10/70 (14%)
Query: 43 EDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFP----------HIISSHF 92
ED+ +++ D NPLH D A+ F P++HGM L + I + F
Sbjct: 797 EDLAALYRLSGDRNPLHIDPNFAKGAKFPKPILHGMCTYGLSAKALIDKFGMFNEIKARF 856
Query: 93 PGAVYVSQSL 102
G V+ ++L
Sbjct: 857 TGIVFPGETL 866
>MAOC_KLEAE (P49251) MAOC protein
Length = 335
Score = 32.7 bits (73), Expect = 0.40
Identities = 26/91 (28%), Positives = 42/91 (45%), Gaps = 4/91 (4%)
Query: 28 LKPGD-VLRKARAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPH 86
++PGD +L R TE D+++++ ++ D H D AA F +VHG + S
Sbjct: 201 IQPGDSLLTPRRTLTEADIVNFACLSGDHFYAHMDKIAAAESIFGERVVHGYFLISAAAG 260
Query: 87 IISSHFPGAV---YVSQSLNFKFPVYIGDQV 114
+ V Y ++L F PV GD +
Sbjct: 261 LFVDAGVRPVIANYGMENLRFIEPVKPGDTI 291
>FAS1_YARLI (P34229) Fatty acid synthase subunit beta (EC 2.3.1.86)
[Includes: 3-hydroxypalmitoyl-[acyl-carrier-protein]
dehydratase (EC 4.2.1.61); Enoyl-[acyl-carrier-protein]
reductase [NADH] (EC 1.3.1.9); [Acyl-carrier-protein]
acetyltransferase (EC 2.
Length = 2086
Score = 32.3 bits (72), Expect = 0.52
Identities = 29/116 (25%), Positives = 49/116 (42%), Gaps = 8/116 (6%)
Query: 48 YSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPHIISSHFPGAVYVSQ----SLN 103
Y+ V+ D NP+H A A G + HGM ++ +I + VS+ S
Sbjct: 1584 YAGVSGDYNPIHVSRAFASYASLPGTITHGMYSSAAVRSLIEV-WAAENNVSRVRAFSCQ 1642
Query: 104 FKFPVYIGDQVIGEVQATNLRANKNRYLVKFKTRCIKSDELVAIEGEAVAMLPTLT 159
F+ V D+++ ++ + R ++K T + E V + GEA P T
Sbjct: 1643 FQGMVLPNDEIVTRLEHVGM--INGRKIIKV-TSTNRETEAVVLSGEAEVEQPIST 1695
>FAPR_THETN (Q8R9V5) Transcription factor fapR (Fatty acid and
phospholipid biosynthesis regulator)
Length = 200
Score = 32.3 bits (72), Expect = 0.52
Identities = 13/45 (28%), Positives = 25/45 (54%)
Query: 93 PGAVYVSQSLNFKFPVYIGDQVIGEVQATNLRANKNRYLVKFKTR 137
P A+ ++ +K+PV +GD+++ + + R NK VK K +
Sbjct: 126 PAALIGVANIKYKYPVKVGDRLVAKAEVIRQRGNKYFVWVKIKVK 170
>NODN_RHIME (P25200) Nodulation protein N
Length = 161
Score = 31.2 bits (69), Expect = 1.2
Identities = 25/93 (26%), Positives = 38/93 (39%), Gaps = 8/93 (8%)
Query: 48 YSKVTHDLNPLHTD-SAAAQNVGFEGPLVHGMLVASLFPHIISSHFP-------GAVYVS 99
++ THD +H D + AA F G + HG L +L + S P G Y
Sbjct: 33 FADATHDHQFIHVDPNRAAAESPFGGAIAHGFLTLALLSVMNFSGMPKFREQTMGINYGF 92
Query: 100 QSLNFKFPVYIGDQVIGEVQATNLRANKNRYLV 132
+ F PV G +V G ++ R + L+
Sbjct: 93 DRVRFISPVRTGSRVHGRFVLSDCRLRRASILM 125
>FAS1_CANAL (P34731) Fatty acid synthase subunit beta (EC 2.3.1.86)
[Includes: 3-hydroxypalmitoyl-[acyl-carrier-protein]
dehydratase (EC 4.2.1.61); Enoyl-[acyl-carrier-protein]
reductase [NADH] (EC 1.3.1.9); [Acyl-carrier-protein]
acetyltransferase (EC 2.
Length = 2037
Score = 31.2 bits (69), Expect = 1.2
Identities = 33/135 (24%), Positives = 56/135 (41%), Gaps = 9/135 (6%)
Query: 28 LKPGDVLRKARAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPHI 87
L G+ L T E Y+ V+ D NP+H A G + HGM ++ +
Sbjct: 1522 LSSGEELTSKAPGTNEP---YAIVSGDYNPIHVSRVFAAYAKLPGTITHGMYSSASIRAL 1578
Query: 88 ISSHFPGAVYVSQSLNFKFPVYIGDQVIGEVQATNLR---ANKNRYLVKFKTRCIKSDEL 144
+ V ++ FK ++G + + T + R ++K +TR +++ EL
Sbjct: 1579 VEEWAANNV-AARVRAFKCD-FVGMVLPNDTLQTTMEHVGMINGRKIIKVETRNVET-EL 1635
Query: 145 VAIEGEAVAMLPTLT 159
+ GEA PT T
Sbjct: 1636 PVLIGEAEIEQPTTT 1650
>NEK4_MOUSE (Q9Z1J2) Serine/threonine-protein kinase Nek4 (EC
2.7.1.37) (NimA-related protein kinase 4)
(Serine/threonine-protein kinase 2)
Length = 792
Score = 30.8 bits (68), Expect = 1.5
Identities = 20/59 (33%), Positives = 28/59 (46%), Gaps = 1/59 (1%)
Query: 3 IRSLVSSKLQSLSLRCFSSAATPQVLKPGDVLRKARAFTEEDVLHYSKVTHDLNPLHTD 61
++ LV Q+L L S QVL PG R R + + VLH KV ++ P T+
Sbjct: 657 MKDLVQLMTQTLRLEAKESCEDLQVLNPGSEFRLHRKYRDTLVLH-GKVAEEVEPHCTE 714
>FXE1_HUMAN (O00358) Forkhead box protein E1 (Thyroid transcription
factor 2) (TTF-2) (Forkhead-related protein FKHL15)
Length = 376
Score = 29.3 bits (64), Expect = 4.4
Identities = 24/86 (27%), Positives = 34/86 (38%), Gaps = 9/86 (10%)
Query: 27 VLKPGDVLRKARAFTEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLFPH 86
+ + G LR+ + F D+ Y HD +AAA + G + A+ P
Sbjct: 136 MFESGSFLRRRKRFKRSDLSTYPAYMHDAAAAAAAAAAAAAAAAAAAIFPGAVPAARPP- 194
Query: 87 IISSHFPGAVYVS---QSLNFKFPVY 109
+PGAVY SL PVY
Sbjct: 195 -----YPGAVYAGYAPPSLAAPPPVY 215
>FABZ_BRUSU (Q8G0E4) (3R)-hydroxymyristoyl-[acyl carrier protein]
dehydratase (EC 4.2.1.-) ((3R)-hydroxymyristoyl ACP
dehydrase)
Length = 157
Score = 29.3 bits (64), Expect = 4.4
Identities = 22/66 (33%), Positives = 32/66 (48%), Gaps = 9/66 (13%)
Query: 93 PGAVYVSQSLNFKF--PVYIGDQVIGEVQATNLRANKNRYLVKFKTRCI-KSDELVAIEG 149
PG VY N KF PV GD+++ V+ RAN ++Y C+ + D + E
Sbjct: 91 PGVVYFMTIDNAKFRRPVVPGDRLLLHVKKIKQRANISKY------ECVAEVDGVKVAEA 144
Query: 150 EAVAML 155
E AM+
Sbjct: 145 EVAAMI 150
>TRYP_SQUAC (P00764) Trypsin precursor (EC 3.4.21.4)
Length = 229
Score = 28.9 bits (63), Expect = 5.8
Identities = 20/82 (24%), Positives = 35/82 (42%), Gaps = 4/82 (4%)
Query: 86 HIISSHFPGAVYVSQSLNFKFPVYIGDQVIGEVQATNLRA----NKNRYLVKFKTRCIKS 141
H IS++ Y+ S+ + P Y G + ++ L N+N L+ T C +
Sbjct: 60 HDISANEGDETYIDSSMVIRHPNYSGYDLDNDIMLIKLSKPAALNRNVDLISLPTGCAYA 119
Query: 142 DELVAIEGEAVAMLPTLTVEQL 163
E+ I G M ++ +QL
Sbjct: 120 GEMCLISGWGNTMDGAVSGDQL 141
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.134 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,490,386
Number of Sequences: 164201
Number of extensions: 652965
Number of successful extensions: 1499
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 1483
Number of HSP's gapped (non-prelim): 27
length of query: 168
length of database: 59,974,054
effective HSP length: 102
effective length of query: 66
effective length of database: 43,225,552
effective search space: 2852886432
effective search space used: 2852886432
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0031.15