BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0030.13
(117 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UPL1_ARATH (Q8GY23) E3 ubiquitin protein ligase UPL1 (EC 6.3.2.-... 31 0.44
UPL2_ARATH (Q8H0T4) E3 ubiquitin protein ligase UPL2 (EC 6.3.2.-... 29 1.7
METE_YEAST (P05694) 5-methyltetrahydropteroyltriglutamate--homoc... 29 2.2
CXA5_CANFA (P33725) Gap junction alpha-5 protein (Connexin 40) (... 28 2.8
MTHC_DROME (P83119) Probable G-protein-coupled receptor Mth-like... 28 3.7
CXA5_RAT (P28234) Gap junction alpha-5 protein (Connexin 40) (Cx40) 28 3.7
CXA5_MOUSE (Q01231) Gap junction alpha-5 protein (Connexin 40) (... 28 3.7
TYR1_HUMAN (P17643) 5,6-dihydroxyindole-2-carboxylic acid oxidas... 27 8.3
MOBA_STAEP (Q8CNE5) Molybdopterin-guanine dinucleotide biosynthe... 27 8.3
>UPL1_ARATH (Q8GY23) E3 ubiquitin protein ligase UPL1 (EC 6.3.2.-)
(Ubiquitin-protein ligase 1)
Length = 3684
Score = 31.2 bits (69), Expect = 0.44
Identities = 21/89 (23%), Positives = 39/89 (43%), Gaps = 3/89 (3%)
Query: 19 HDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPFSISNIVQYVYMPILSQQD 78
H L+ + + ++ S RK K H ++ IP + ++ + P L Q
Sbjct: 2894 HSAVADMLFYFDSSLLSQLSS-RKGKEKVTHVTDSRDLEIPLVV--FLKLLNRPQLLQST 2950
Query: 79 YHLGRLQRLVEDYVHVVADKYPYWNRSNG 107
HLG + L++ V+ A + W+ S+G
Sbjct: 2951 SHLGLVMGLLQVVVYTAASRIEGWSPSSG 2979
>UPL2_ARATH (Q8H0T4) E3 ubiquitin protein ligase UPL2 (EC 6.3.2.-)
(Ubiquitin-protein ligase 2)
Length = 3658
Score = 29.3 bits (64), Expect = 1.7
Identities = 20/89 (22%), Positives = 38/89 (42%), Gaps = 3/89 (3%)
Query: 19 HDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPFSISNIVQYVYMPILSQQD 78
H L+ + + ++ S RK K H ++ IP + ++ + P L Q
Sbjct: 2877 HSAVADMLFYFDSSLLSQLSS-RKGKEKVTHETDSRDLEIPLVV--FLKLLNRPQLLQST 2933
Query: 79 YHLGRLQRLVEDYVHVVADKYPYWNRSNG 107
HL + L++ V+ A + W+ S+G
Sbjct: 2934 SHLALVMGLLQVVVYTAASRIEGWSPSSG 2962
>METE_YEAST (P05694)
5-methyltetrahydropteroyltriglutamate--homocysteine
methyltransferase (EC 2.1.1.14) (Methionine synthase,
vitamin-B12 independent isozyme) (Cobalamin-independent
methionine synthase) (Delta-P8 protein)
Length = 766
Score = 28.9 bits (63), Expect = 2.2
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 4/51 (7%)
Query: 9 VYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIP 59
V G+ +HD +K + +DE S R +PF+ R P++ +F +P
Sbjct: 390 VESRGKSKFIHDAAVKARVAS----IDEKMSTRAAPFEQRLPEQQKVFNLP 436
>CXA5_CANFA (P33725) Gap junction alpha-5 protein (Connexin 40)
(Cx40)
Length = 356
Score = 28.5 bits (62), Expect = 2.8
Identities = 18/64 (28%), Positives = 32/64 (49%), Gaps = 9/64 (14%)
Query: 25 GLYSIEGQFMDEIDSCRKSPFK------ARHPDEANLFLIPFSISNIVQYVYMPILSQQD 78
G Y + G F+D + CR+SP P E N+F++ F ++ +++ + +
Sbjct: 168 GQYLLYGIFLDTLHVCRRSPCPHPVNCYVSRPTEKNVFIV-FMLAVAALSLFLSL--AEL 224
Query: 79 YHLG 82
YHLG
Sbjct: 225 YHLG 228
>MTHC_DROME (P83119) Probable G-protein-coupled receptor Mth-like 12
precursor (Methuselah-like 12 protein)
Length = 488
Score = 28.1 bits (61), Expect = 3.7
Identities = 16/51 (31%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Query: 47 ARHPDEANLFLIPFSISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVAD 97
A+H DE N + F++ +Q+V + ++ + L +L RL ED H++ D
Sbjct: 405 AQH-DERNTTCLEFNVQTYIQFVRLFLIMGASWLLDQLTRLAED-SHLLLD 453
>CXA5_RAT (P28234) Gap junction alpha-5 protein (Connexin 40) (Cx40)
Length = 355
Score = 28.1 bits (61), Expect = 3.7
Identities = 18/65 (27%), Positives = 32/65 (48%), Gaps = 11/65 (16%)
Query: 25 GLYSIEGQFMDEIDSCRKSPFK------ARHPDEANLFLI-PFSISNIVQYVYMPILSQQ 77
G Y + G F+D + CR+SP P E N+F++ +++ + ++ + L
Sbjct: 168 GQYLLYGIFLDTLHVCRRSPCPHPVNCYVSRPTEKNVFIVFMMAVAGLSLFLSLAEL--- 224
Query: 78 DYHLG 82
YHLG
Sbjct: 225 -YHLG 228
>CXA5_MOUSE (Q01231) Gap junction alpha-5 protein (Connexin 40)
(Cx40)
Length = 357
Score = 28.1 bits (61), Expect = 3.7
Identities = 18/65 (27%), Positives = 32/65 (48%), Gaps = 11/65 (16%)
Query: 25 GLYSIEGQFMDEIDSCRKSPFK------ARHPDEANLFLI-PFSISNIVQYVYMPILSQQ 77
G Y + G F+D + CR+SP P E N+F++ +++ + ++ + L
Sbjct: 169 GQYLLYGIFLDTLHVCRRSPCPHPVNCYVSRPTEKNVFIVFMMAVAGLSLFLSLAEL--- 225
Query: 78 DYHLG 82
YHLG
Sbjct: 226 -YHLG 229
>TYR1_HUMAN (P17643) 5,6-dihydroxyindole-2-carboxylic acid oxidase
precursor (EC 1.14.18.-) (DHICA oxidase)
(Tyrosinase-related protein 1) (TRP-1) (TRP1) (TRP)
(Catalase B) (Glycoprotein-75) (Melanoma antigen gp75)
Length = 537
Score = 26.9 bits (58), Expect = 8.3
Identities = 11/34 (32%), Positives = 20/34 (58%)
Query: 74 LSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNG 107
L+ YHL RL++ +++ + + PYWN + G
Sbjct: 221 LTWHRYHLLRLEKDMQEMLQEPSFSLPYWNFATG 254
>MOBA_STAEP (Q8CNE5) Molybdopterin-guanine dinucleotide
biosynthesis protein A
Length = 201
Score = 26.9 bits (58), Expect = 8.3
Identities = 10/16 (62%), Positives = 13/16 (80%)
Query: 21 GPLKGLYSIEGQFMDE 36
GPL G+YS+ Q+MDE
Sbjct: 73 GPLTGIYSVMKQYMDE 88
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.142 0.448
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,981,732
Number of Sequences: 164201
Number of extensions: 569092
Number of successful extensions: 1168
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1165
Number of HSP's gapped (non-prelim): 9
length of query: 117
length of database: 59,974,054
effective HSP length: 93
effective length of query: 24
effective length of database: 44,703,361
effective search space: 1072880664
effective search space used: 1072880664
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0030.13