BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0027.9
(1611 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog 106 5e-22
LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog 104 2e-21
POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains... 96 1e-18
M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310... 84 4e-15
MC50_ARATH (P92555) Hypothetical mitochondrial protein AtMg01250... 67 3e-10
POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type... 67 5e-10
YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein ... 57 5e-07
PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type... 49 1e-04
PO21_NASVI (Q03278) Retrovirus-related Pol polyprotein from type... 43 0.006
PO22_POPJA (Q03274) Retrovirus-related Pol polyprotein from type... 42 0.018
LPXD_RALSO (Q8XZI1) UDP-3-O-[3-hydroxymyristoyl] glucosamine N-a... 33 6.3
REB1_KLULA (Q05950) DNA-binding protein REB1 (QBP) 33 8.3
>LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog
Length = 1260
Score = 106 bits (265), Expect = 5e-22
Identities = 79/290 (27%), Positives = 141/290 (48%), Gaps = 17/290 (5%)
Query: 1095 PPQVATLQFFGFDDKFIELIKWCISSSSMMISWRGNYSESFNPSRGVRQGDPISPYLFVL 1154
P + TL+ G + F++LI+ S + I G +SF G RQG P+SP LF +
Sbjct: 610 PFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNI 669
Query: 1155 CIERLALTIKDACSDGRWKAFKVGRSGPSISHLLFADDVVLFAEASVDQAILIDEILHNF 1214
+E LA+ I++ + K +G +S LFADD++++ E + D + E++ +
Sbjct: 670 VMEVLAIAIRE---EKAIKGIHIGSEEIKLS--LFADDMIVYLENTRDSTTKLLEVIKEY 724
Query: 1215 CEASGQKVSPPKSRVFFSKNVHDGK---ADQIVHALGIQKTTNLGMYLGVPLLHSILSKE 1271
SG K++ KS F N + + D I + +K LG+YL + L KE
Sbjct: 725 SNVSGYKINTHKSVAFIYTNNNQAEKTVKDSIPFTVVPKKMKYLGVYLTKDV--KDLYKE 782
Query: 1272 NFNFLIDKVRKKLSGWKVSTLSFAGRVSLTQSCLLSLPAYVMQTTPI--PIGVCQEIERL 1329
N+ L ++ + ++ WK S+ GR+++ + +L Y PI P+ +++E++
Sbjct: 783 NYETLRKEIAEDVNKWKNIPCSWLGRINIVKMSILPKAIYNFNAIPIKAPLSYFKDLEKI 842
Query: 1330 CRNFIWGSTADHRKCHLISWDTICSPKDQGGLGFCDLRTINQAYMAKLAW 1379
+FIW ++K I+ + + GG+ DLR ++ + K AW
Sbjct: 843 ILHFIW-----NQKKPQIAKTLLSNKNKAGGITLPDLRLYYKSIVIKTAW 887
>LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog
Length = 1259
Score = 104 bits (259), Expect = 2e-21
Identities = 77/297 (25%), Positives = 139/297 (45%), Gaps = 15/297 (5%)
Query: 1094 RPPQVATLQFFGFDDKFIELIKWCISSSSMMISWRGNYSESFNPSRGVRQGDPISPYLFV 1153
+P + L G D ++++I+ + I G E+ G RQG P+SP L
Sbjct: 609 QPFMLKPLNKLGIDGTYLKIIRAIYDKPTANIILNGQKLEAPPLKTGTRQGCPLSPLLPN 668
Query: 1154 LCIERLALTIKDACSDGRWKAFKVGRSGPSISHLLFADDVVLFAEASVDQAILIDEILHN 1213
+ +E LA I+ + K ++G+ +S LFADD++++ E + A + +++ N
Sbjct: 669 IVLEVLARAIRQ---EKEIKGIQLGKEEVKLS--LFADDMIVYLENPIVSAQNLLKLISN 723
Query: 1214 FCEASGQKVSPPKSRVFFSKNVHDGKADQIVHALGIQKTTNLGMYLGVPLLHSI--LSKE 1271
F + SG K++ KS+ F N ++ QI+ L + YLG+ L + L KE
Sbjct: 724 FSKVSGYKINVQKSQAFLYTNNRQTES-QIMSELPFTIASKRIKYLGIQLTRDVKDLFKE 782
Query: 1272 NFNFLIDKVRKKLSGWKVSTLSFAGRVSLTQSCLLSLPAYVMQTTPI--PIGVCQEIERL 1329
N+ L++++++ + WK S+ GR+++ + +L Y PI P+ E+E+
Sbjct: 783 NYKPLLNEIKEDTNKWKNIPCSWVGRINIVKMAILPKVIYRFNAIPIKLPMTFFTELEKT 842
Query: 1330 CRNFIWGSTADHRKCHLISWDTICSPKDQGGLGFCDLRTINQAYMAKLAWNILADKD 1386
FIW H I+ T+ GG+ D + +A + K AW ++D
Sbjct: 843 TLKFIWNQKRAH-----IAKSTLSQKNKAGGITLPDFKLYYKATVTKTAWYWYQNRD 894
>POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains:
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1300
Score = 95.5 bits (236), Expect = 1e-18
Identities = 80/304 (26%), Positives = 133/304 (43%), Gaps = 18/304 (5%)
Query: 1095 PPQVATLQFFGFDDKFIELIKWCISSSSMMISWRGNYSESFNPSRGVRQGDPISPYLFVL 1154
P + L+ G ++ +IK S I G E+ G RQG P+SPYLF +
Sbjct: 637 PFMIKVLERSGIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNI 696
Query: 1155 CIERLALTIKDACSDGRWKAFKVGRSGPSISHLLFADDVVLFAEASVDQAILIDEILHNF 1214
+E LA I+ K ++G+ IS L ADD++++ + + ++++F
Sbjct: 697 VLEVLARAIR---QQKEIKGIQIGKEEVKIS--LLADDMIVYISDPKNSTRELLNLINSF 751
Query: 1215 CEASGQKVSPPKSRVF-FSKNVHDGKADQIVHALGIQKTTNLGMYLGVPLLHSI--LSKE 1271
E G K++ KS F ++KN K +I TN YLGV L + L +
Sbjct: 752 GEVVGYKINSNKSMAFLYTKNKQAEK--EIRETTPFSIVTNNIKYLGVTLTKEVKDLYDK 809
Query: 1272 NFNFLIDKVRKKLSGWKVSTLSFAGRVSLTQSCLLSLPAYVMQTTP--IPIGVCQEIERL 1329
NF L ++++ L WK S+ GR+++ + +L Y P IP E+E
Sbjct: 810 NFKSLKKEIKEDLRRWKDLPCSWIGRINIVKMAILPKAIYRFNAIPIKIPTQFFNELEGA 869
Query: 1330 CRNFIWGSTADHRKCHLISWDTICSPKDQGGLGFCDLRTINQAYMAKLAWNILADKD-SL 1388
F+W + K I+ + + GG+ DL+ +A + K AW D+
Sbjct: 870 ICKFVW-----NNKKPRIAKSLLKDKRTSGGITMPDLKLYYRAIVIKTAWYWYRDRQVDQ 924
Query: 1389 WTQV 1392
W ++
Sbjct: 925 WNRI 928
>M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310
(ORF154)
Length = 154
Score = 83.6 bits (205), Expect = 4e-15
Identities = 44/151 (29%), Positives = 78/151 (51%), Gaps = 2/151 (1%)
Query: 1306 LSLPAYVMQTTPIPIGVCQEIERLCRNFIWGSTADHRKCHLISWDTICSPK-DQGGLGFC 1364
++LP Y M + +C+++ F W S + RK ++W +C K D GGLGF
Sbjct: 1 MALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFR 60
Query: 1365 DLRTINQAYMAKLAWNILADKDSLWTQVLKAKYKFNPGLRAPPLPKPPDSPTWKGICKAW 1424
DL NQA +AK ++ I+ +L +++L+++Y + + + P S W+ I
Sbjct: 61 DLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRP-SYAWRSIIHGR 119
Query: 1425 PIMEKGVKWTLGNGIKVSFWRDNWIPNMGPI 1455
++ +G+ T+G+GI W D WI + P+
Sbjct: 120 ELLSRGLLRTIGDGIHTKVWLDRWIMDETPL 150
>MC50_ARATH (P92555) Hypothetical mitochondrial protein AtMg01250
(ORF102)
Length = 122
Score = 67.4 bits (163), Expect = 3e-10
Identities = 31/56 (55%), Positives = 39/56 (69%)
Query: 1137 PSRGVRQGDPISPYLFVLCIERLALTIKDACSDGRWKAFKVGRSGPSISHLLFADD 1192
PSRG+RQGDP+SPYLF+LC E L+ + A GR +V + P I+HLLFADD
Sbjct: 24 PSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRINHLLFADD 79
>POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type II
retrotransposable element R2DM [Contains: Protease (EC
3.4.23.-); Reverse transcriptase (EC 2.7.7.49);
Endonuclease]
Length = 1057
Score = 66.6 bits (161), Expect = 5e-10
Identities = 71/288 (24%), Positives = 126/288 (43%), Gaps = 43/288 (14%)
Query: 1100 TLQFFGFDDKFIELIKWCISSSSMMISWRGNYSESFNPSRGVRQGDPISPYLFVLCIERL 1159
TL+ +G F++ ++ ++ G SE F P+RGV+QGDP+SP LF L ++RL
Sbjct: 498 TLRAYGAPKGFVDYVQNTYEGGGTSLNGDGWSSEEFVPARGVKQGDPLSPILFNLVMDRL 557
Query: 1160 ALTIKDACSDGRWKAFKVGRSGPSISHLLFADDVVLFAEASVDQAILIDEILHNFCEASG 1219
T+ KVG + + + FADD+VLFAE + +L+D+ L +F G
Sbjct: 558 LRTLPSEI------GAKVGNAITNAA--AFADDLVLFAETRMGLQVLLDKTL-DFLSIVG 608
Query: 1220 QKVSPPKSRVFFSKNVHDGKADQIVHALGIQKTTNLGMYLGVPLLHSILSKENFNFL--- 1276
K++ K F+ + G+ Q L Q Y+G + S+ + + +L
Sbjct: 609 LKLNADKC---FTVGI-KGQPKQKCTVLEAQ-----SFYVGSSEIPSLKRTDEWKYLGIN 659
Query: 1277 -----------IDKVRKKLSGWKVSTLSFAGRVSLTQSCLLSLPAYVMQTTPIPIGVCQE 1325
+ + KL + L R+ ++ L+ + + + IGV ++
Sbjct: 660 FTATGRVRCNPAEDIGPKLQRLTKAPLKPQQRLFALRTVLIPQLYHKLALGSVAIGVLRK 719
Query: 1326 IERLCRNFIWGSTADHRKCHLISWDT----ICSPKDQGGLGFCDLRTI 1369
++L R ++ R+ + D + +P GGLG LR +
Sbjct: 720 TDKLIRYYV-------RRWLNLPLDVPIAFVHAPPKSGGLGIPSLRWV 760
>YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein (ORF
2)
Length = 1308
Score = 56.6 bits (135), Expect = 5e-07
Identities = 70/306 (22%), Positives = 122/306 (38%), Gaps = 20/306 (6%)
Query: 1098 VATLQFFGFDDKFIELIKWCISSSSMMISWRGNYSESFNPSRGVRQGDPISPYLFVLCIE 1157
+ TLQ + F +F+ +K +S+ ++ + + RGVRQG P+S L+ L IE
Sbjct: 608 IGTLQAYSFGPQFVGYLKTMYASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIE 667
Query: 1158 RLALTIKDACSDGRWKAFKVGRSGPSISHLLFADDVVLFAEASVDQAILIDEILHNFCEA 1217
++ R + + +ADDV+L A+ VD E + A
Sbjct: 668 PFLCLLRK-----RLTGLVLKEPDMRVVLSAYADDVILVAQDLVDLE-RAQECQEVYAAA 721
Query: 1218 SGQKVSPPKSRVFFSKNVHDGKADQIVHAL-GIQKTTNLGMYLGVPL-LHSILSKENFNF 1275
S +++ KS ++ K D + A I + + YLGV L +NF
Sbjct: 722 SSARINWSKSSGLLEGSL---KVDFLPPAFRDISWESKIIKYLGVYLSAEEYPVSQNFIE 778
Query: 1276 LIDKVRKKLSGWK--VSTLSFAGRVSLTQSCLLSLPAYVMQTTPIPIGVCQEIERLCRNF 1333
L + V +L WK LS GR + + S Y + +I+R +F
Sbjct: 779 LEECVLTRLGKWKGFAKVLSMRGRALVINQLVASQIWYRLICLSPTQEFIAKIQRRLLDF 838
Query: 1334 IWGSTADHRKCHLISWDTICSPKDQGGLGFCDLRT-INQAYMAKLAWNILADKDSLWTQV 1392
+W H +S P +GG G +R+ ++ + ++ + AD W +
Sbjct: 839 LWIGK------HWVSAGVSSLPLKEGGQGVVCIRSQVHTFRLQQIQRYLYADPSPQWCTL 892
Query: 1393 LKAKYK 1398
+ Y+
Sbjct: 893 ASSFYR 898
>PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 606
Score = 48.5 bits (114), Expect = 1e-04
Identities = 66/309 (21%), Positives = 119/309 (38%), Gaps = 28/309 (9%)
Query: 1095 PPQVATLQFFGFDDKFIELIKWCISSSSMMISWRGNYSESFNPSRGVRQGDPISPYLFVL 1154
P + ++ FG DD + I I+ + I G + GV+QGDP+SP LF +
Sbjct: 40 PAILRAMRAFGIDDGMQDFIMSTITDAYTNIVVGGRTTNKIYIRNGVKQGDPLSPVLFNI 99
Query: 1155 CIERLALTIKDACSDGRWKAFKVGRSGPSISHLLFADDVVLFAEASVDQAILIDEILHNF 1214
++ L + D I+ L FADD++L + +D + L
Sbjct: 100 VLDELVTRLNDEQPGASM------TPACKIASLAFADDLLLLEDRDID----VPNSLATT 149
Query: 1215 C---EASGQKVSPPK-----SRVFFSKNVHDGKADQIVHALGIQKT--TNLGMYLGVPLL 1264
C G ++P K + ++V K + I+ N YLG+
Sbjct: 150 CAYFRTRGMTLNPEKCASISAATVSGRSVPRSKPSFTIDGRYIKPLGGINTFKYLGLTFS 209
Query: 1265 HSILSKENFNFLIDKVRKKLSGWKVSTLSFAGRVSLTQSCLLSLPAYVMQTTPIPIGVCQ 1324
+ ++K + + + L + + L + + ++ LL Y +Q+ + G+ Q
Sbjct: 210 STGVAKPT----VYNLTRWLRNLEKAPLKPNQKFYILKTHLLPRLFYGLQSPGVTAGILQ 265
Query: 1325 EIERLCRNFIWGSTADHRKCHLISWDTICSPKDQGGLGFCDLRTINQAYMAKLAWNILAD 1384
E +RL R H H S +D GGLG +R ++K ++
Sbjct: 266 ECDRLARRTT--RKIFHLNVHTGSQFLHARIRD-GGLGLVQMRYRIPCILSKRLGSLKQG 322
Query: 1385 KDSL-WTQV 1392
D+ W+ +
Sbjct: 323 NDTTNWSDI 331
>PO21_NASVI (Q03278) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 1025
Score = 43.1 bits (100), Expect = 0.006
Identities = 46/159 (28%), Positives = 64/159 (39%), Gaps = 22/159 (13%)
Query: 1114 IKWCISSSSMMISWRGNYSESFNPSRGVRQGDPISPYLFVLCIERLALTIKDACSDGRWK 1173
I W +S + P+RGVRQGDP+SP LF ++ + + +
Sbjct: 486 IMWVYRNSKTRLEVVKTKGRWIRPARGVRQGDPLSPLLFNCVMDAVLRRLPEN------T 539
Query: 1174 AFKVGRSGPSISHLLFADDVVLFAEASVDQAILIDEILHNFCEASGQKVSPPKSRVFFSK 1233
F +G I L+FADD+VL AE + I E G ++ P K
Sbjct: 540 GFLMG--AEKIGALVFADDLVLLAETREGLQASLSRIEAGLQE-QGLEMMPRKCHTL--A 594
Query: 1234 NVHDGKADQI------VHALGIQKTTNLG-----MYLGV 1261
V GK +I +G Q+ T LG YLGV
Sbjct: 595 LVPSGKEKKIKVETHKPFTVGNQEITQLGHADQWKYLGV 633
>PO22_POPJA (Q03274) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 711
Score = 41.6 bits (96), Expect = 0.018
Identities = 37/129 (28%), Positives = 57/129 (43%), Gaps = 10/129 (7%)
Query: 1101 LQFFGFDDKFIELIKWCISSSSMMIS-WRGNYSESFNPSRGVRQGDPISPYLFVLCIERL 1159
LQ G D+ I +S S+ I G+ + RGV+QGDP+SP+LF ++ L
Sbjct: 160 LQRLGIDEGTSNYITGSLSDSTTTIRVGPGSQTRKICIRRGVKQGDPLSPFLFNAVLDEL 219
Query: 1160 ALTIKDACSDGRWKAFKVGRSG-PSISHLLFADDVVLFAEASVDQAILIDEILHNFCEAS 1218
+++ G G G I L FADD++L + V + + NF
Sbjct: 220 LCSLQSTPGIG-------GTIGEEKIPVLAFADDLLLLEDNDVLLPTTLATVA-NFFRLR 271
Query: 1219 GQKVSPPKS 1227
G ++ KS
Sbjct: 272 GMSLNAKKS 280
>LPXD_RALSO (Q8XZI1) UDP-3-O-[3-hydroxymyristoyl] glucosamine
N-acyltransferase (EC 2.3.1.-)
Length = 356
Score = 33.1 bits (74), Expect = 6.3
Identities = 19/77 (24%), Positives = 31/77 (39%), Gaps = 8/77 (10%)
Query: 1182 PSISHLLFADDVVLFAEASVDQAILIDEILHNFCEASGQKVSPPKSRVFFSKNVHDGKAD 1241
P + DDV + A ++D+ + D ++ C+ Q V + NVH G
Sbjct: 208 PQTGRAIVGDDVEIGANTAIDRGAMADTVVEQGCKIDNQ--------VQIAHNVHVGAYT 259
Query: 1242 QIVHALGIQKTTNLGMY 1258
I I +T +G Y
Sbjct: 260 VIAGCAAISGSTKIGRY 276
>REB1_KLULA (Q05950) DNA-binding protein REB1 (QBP)
Length = 595
Score = 32.7 bits (73), Expect = 8.3
Identities = 21/105 (20%), Positives = 44/105 (41%), Gaps = 15/105 (14%)
Query: 1366 LRTINQAYMAKLAWNILADKDSLWTQVLKAKYKFNPGLRAPPLPKPPDSPTWKGICKAWP 1425
+R +++ + + W+ KD WT + + LP S +K + + +
Sbjct: 286 IRGLSRRQICERIWSNERRKDDFWTNICRV------------LPYRTRSSIYKHVRRKYH 333
Query: 1426 IMEKGVKWTLGNGIKVSFW---RDNWIPNMGPITSFMPDVHSEAW 1467
I E+ KWT +++ W ++ N+G + MP+ + W
Sbjct: 334 IFEQRGKWTPEEDAELARWCAEKEGQWSNIGKVLGRMPEDCRDRW 378
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.341 0.150 0.537
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 186,618,803
Number of Sequences: 164201
Number of extensions: 7893754
Number of successful extensions: 21860
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 21831
Number of HSP's gapped (non-prelim): 18
length of query: 1611
length of database: 59,974,054
effective HSP length: 124
effective length of query: 1487
effective length of database: 39,613,130
effective search space: 58904724310
effective search space used: 58904724310
T: 11
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 73 (32.7 bits)
Lotus: description of TM0027.9