Lotus
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0023.9
         (388 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310...    88  3e-17
Y939_SULTO (Q973G3) Hypothetical protein ST0939 precursor              32  2.1
YD16_METTH (O27371) Hypothetical UPF0215 protein MTH1316               32  2.8
AKT6_ARATH (Q8GXE6) Potassium channel AKT6 (Shaker pollen inward...    31  4.7
GATE_METJA (Q60325) Glutamyl-tRNA(Gln) amidotransferase subunit ...    30  8.0

>M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310
           (ORF154)
          Length = 154

 Score = 88.2 bits (217), Expect = 3e-17
 Identities = 52/152 (34%), Positives = 75/152 (49%), Gaps = 7/152 (4%)

Query: 118 AIPSYIMSCFILPDSICADIDRMIACFFWGGDVTRRGLHWTRWANLCKNKR-DGGLGFRF 176
           A+P Y MSCF L   +C  +   +  F+W     +R + W  W  LCK+K  DGGLGFR 
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 177 FRAFKEALVAKN**RIYMFPESLMGRVFKVVYSPSSSILMAKKG---YITWSSILKAGGK 233
              F +AL+AK   RI   P +L+ R+ +  Y P SS++    G      W SI+     
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 234 IEGGGGGLRWRVGDGFQINLLQDKWLPDGSSL 265
           +     GL   +GDG    +  D+W+ D + L
Sbjct: 122 L---SRGLLRTIGDGIHTKVWLDRWIMDETPL 150


>Y939_SULTO (Q973G3) Hypothetical protein ST0939 precursor
          Length = 709

 Score = 32.3 bits (72), Expect = 2.1
 Identities = 16/48 (33%), Positives = 25/48 (51%), Gaps = 2/48 (4%)

Query: 292 ELLEEVFSSSPMQKILAIPLSNQGNTDALYWYDTFNGVYVCKSGYKFI 339
           +++    SS P   I+ + + N   T  L WYDTFNG Y  +  Y ++
Sbjct: 393 DIISATPSSVPNPPIIKVKIGNLNAT--LTWYDTFNGGYPIEGYYLYV 438


>YD16_METTH (O27371) Hypothetical UPF0215 protein MTH1316
          Length = 204

 Score = 32.0 bits (71), Expect = 2.8
 Identities = 25/83 (30%), Positives = 37/83 (44%), Gaps = 6/83 (7%)

Query: 60  VKEVKSFDKYLGLPTIIGKSKTQIFNFVKERIWKRLKGWKE--KSLSRAGREILIKSIAQ 117
           V ++    +  GLP I+   K      +K  +  R   WK   +S+ RAGR   I  +  
Sbjct: 96  VVDIVELSERTGLPVIVVVRKHPDMERIKRALKHRFSDWKARWRSIERAGR---IHRVES 152

Query: 118 AIPSYIMSCFILPDSICADIDRM 140
             P YI +  I PD   A+I R+
Sbjct: 153 REPLYIQTAGIEPDD-AAEIVRI 174


>AKT6_ARATH (Q8GXE6) Potassium channel AKT6 (Shaker pollen inward
           rectifier K(+) channel) (Potassium channel SPIK)
          Length = 888

 Score = 31.2 bits (69), Expect = 4.7
 Identities = 15/32 (46%), Positives = 19/32 (58%)

Query: 256 DKWLPDGSSLVYMHEAIAELRLEKVSDLLDNG 287
           D  LPDG+    +H A++E  LE V  LLD G
Sbjct: 666 DVTLPDGNGTTALHRAVSEGHLEIVKFLLDQG 697


>GATE_METJA (Q60325) Glutamyl-tRNA(Gln) amidotransferase subunit E
           (EC 6.3.5.-) (Glu-ADT subunit E)
          Length = 630

 Score = 30.4 bits (67), Expect = 8.0
 Identities = 30/96 (31%), Positives = 48/96 (49%), Gaps = 11/96 (11%)

Query: 216 MAKKGYITWSSILKAGGKIEGGGGGLRWRVG----DGFQINL--LQDKWLPDGSSLVYMH 269
           MAK+       IL+A GK++ G G +R  +     DG +I +  +QD    D    V  +
Sbjct: 198 MAKEAARRIGEILRATGKVKRGLGTIRQDINISIKDGARIEVKGVQDL---DLIEKVVEN 254

Query: 270 EAIAELRLEKVSDLLDNGRWNLELLEEVFSSSPMQK 305
           E I +L L K+ D L     N E++E++F  + + K
Sbjct: 255 EVIRQLNLLKIRDELRER--NAEVVEKIFDVTEIFK 288


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.334    0.147    0.477 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,819,011
Number of Sequences: 164201
Number of extensions: 1906570
Number of successful extensions: 6383
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 6379
Number of HSP's gapped (non-prelim): 5
length of query: 388
length of database: 59,974,054
effective HSP length: 112
effective length of query: 276
effective length of database: 41,583,542
effective search space: 11477057592
effective search space used: 11477057592
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 67 (30.4 bits)


Lotus: description of TM0023.9