BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.13
(195 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein ... 62 1e-09
LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog 44 2e-04
LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog 39 0.006
YA03_CLOPE (Q8XLN8) Hypothetical UPF0291 protein CPE1003 39 0.010
POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains... 36 0.065
YOI8_BACCR (Q812Y7) Hypothetical UPF0291 protein BC3688 34 0.25
Y1F2_BACAN (Q81Y08) Hypothetical UPF0291 protein BA3752/GBAA3752... 34 0.25
Y1C5_BACC1 (P61462) Hypothetical UPF0291 protein BCE3725 34 0.25
YLAC_LACLA (Q9CGJ9) Hypothetical UPF0291 protein ylaC 33 0.32
YG9A_CLOTE (P60083) Hypothetical UPF0291 protein CTC01690.1 32 0.72
YR26_CLOAB (Q97FL0) Hypothetical UPF0291 protein CAC2726 32 1.2
SYT_METJA (Q58597) Threonyl-tRNA synthetase (EC 6.1.1.3) (Threon... 32 1.2
YG71_OCEIH (Q8EQM4) Hypothetical UPF0291 protein OB1671 31 1.6
Y064_ENTFA (Q839T8) Hypothetical UPF0291 protein EF0064 31 1.6
OPPF_MYCGE (P47326) Oligopeptide transport ATP-binding protein oppF 31 1.6
YD41_STAAM (P60074) Hypothetical UPF0291 protein SAV1341 30 2.7
YC28_STAAW (P60078) Hypothetical UPF0291 protein MW1228 30 2.7
YB76_STAAN (P60076) Hypothetical UPF0291 protein SA1176 30 2.7
YA24_STAEP (Q8CSP4) Hypothetical UPF0291 protein SE1024 30 2.7
Y585_METJA (Q58005) Hypothetical protein MJ0585 30 3.6
>YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein
(ORF 2)
Length = 1308
Score = 61.6 bits (148), Expect = 1e-09
Identities = 39/143 (27%), Positives = 73/143 (50%), Gaps = 3/143 (2%)
Query: 47 YQKSRSKWLKEGDQNTKYFHSLINWRKRTNSIMGLMI-DGNWVEDPQAIKTEVQNYFEQC 105
+ +SR + L + D+ +++F++L + I L DG +EDP+AI+ +++++
Sbjct: 357 FVRSRMQLLCDMDRGSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDRARSFYQNL 416
Query: 106 FTNSLGTLVRLDEI--SFKSISDADNELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKF 163
F+ + +E+ +S+ E L ++E+ A+ +KSP D +F
Sbjct: 417 FSPDPISPDACEELWDGLPVVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEF 476
Query: 164 IQKFWDLLKDDFHRVLEDFWNRG 186
Q FWD L DFHRVL + + +G
Sbjct: 477 FQFFWDTLGPDFHRVLTEAFKKG 499
>LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog
Length = 1259
Score = 43.9 bits (102), Expect = 2e-04
Identities = 37/171 (21%), Positives = 79/171 (45%), Gaps = 10/171 (5%)
Query: 4 VVQKLNELDRKEEEEGLSE--EENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQN 61
++ +L EL+++E+ + +E + + L E T L + +SRS + ++ ++
Sbjct: 316 LISQLKELEKQEQTNSKASRRQEIIKIRAELKEIETQKTLQK---INESRSWFFEKINKI 372
Query: 62 TKYFHSLINWRKRTNSIMGLMID-GNWVEDPQAIKTEVQNYFEQCFTNSLGTLVRLDEI- 119
+ LI ++ N I + D G+ DP I+T ++ Y++ + N L L +D+
Sbjct: 373 DRPLARLIKKKREKNQIDTIKNDRGDITTDPTEIQTTIREYYKHLYANKLENLEEMDKFL 432
Query: 120 ---SFKSISDADNELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKFIQKF 167
+ ++ + E L E++ + KSP P+ F +F Q++
Sbjct: 433 DTYTLPRLNQEEVESLNRPITSSEIEAIINSLPNKKSPGPEGFTAEFYQRY 483
>LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog
Length = 1260
Score = 39.3 bits (90), Expect = 0.006
Identities = 40/176 (22%), Positives = 77/176 (43%), Gaps = 11/176 (6%)
Query: 1 RNEVVQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQ---KSRSKWLKE 57
R EV + L + E+EE S + + RK + + + + Q KS+S + ++
Sbjct: 310 REEVNNLMGHLKQLEKEEH-SNPKPSRRKEITKIRAELNEIENKRIIQQINKSKSWFFEK 368
Query: 58 GDQNTKYFHSLINWRKRTNSIMGLMIDGN--WVEDPQAIKTEVQNYFEQCFTNSLGTLVR 115
++ K +L +KR S++ + +GN DP I+ + Y+++ +++ L
Sbjct: 369 INKIDKPLANLTR-KKRVKSLISSIRNGNDEITTDPSEIQKILNEYYKKLYSHKYENLKE 427
Query: 116 LDEI----SFKSISDADNELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKFIQKF 167
+D+ +S + E+L E+ + KSP PD F +F Q F
Sbjct: 428 IDQYLEACHLPRLSQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTF 483
>YA03_CLOPE (Q8XLN8) Hypothetical UPF0291 protein CPE1003
Length = 59
Score = 38.5 bits (88), Expect = 0.010
Identities = 16/34 (47%), Positives = 27/34 (79%)
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERKWLLDEF 35
+E+ +++NEL +K +EEGLSE+E+ ER+ L E+
Sbjct: 4 DELTKRINELHKKHKEEGLSEDEHKEREELRKEY 37
>POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains:
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1300
Score = 35.8 bits (81), Expect = 0.065
Identities = 20/98 (20%), Positives = 46/98 (46%), Gaps = 4/98 (4%)
Query: 85 GNWVEDPQAIKTEVQNYFEQCFTNSLGTLVRLDEI----SFKSISDADNELLTGRFGMEE 140
G+ DP+ I+ +++++++ ++ L L +D+ ++ + L +E
Sbjct: 424 GDITTDPEEIQNTIRSFYKRLYSTKLENLDEMDKFLDRYQVPKLNQDQVDHLNSPISPKE 483
Query: 141 VKGAVWECGGDKSPEPDEFNFKFIQKFWDLLKDDFHRV 178
++ + KSP PD F+ +F Q F + L H++
Sbjct: 484 IEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIPILHKL 521
>YOI8_BACCR (Q812Y7) Hypothetical UPF0291 protein BC3688
Length = 79
Score = 33.9 bits (76), Expect = 0.25
Identities = 14/34 (41%), Positives = 26/34 (76%)
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERKWLLDEF 35
+E+V+++N L +K + EGL+EEE ER+ L +++
Sbjct: 4 HELVERINFLAKKAKAEGLTEEEQRERQSLREQY 37
>Y1F2_BACAN (Q81Y08) Hypothetical UPF0291 protein
BA3752/GBAA3752/BAS3478
Length = 79
Score = 33.9 bits (76), Expect = 0.25
Identities = 14/34 (41%), Positives = 26/34 (76%)
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERKWLLDEF 35
+E+V+++N L +K + EGL+EEE ER+ L +++
Sbjct: 4 HELVERINFLAKKAKAEGLTEEEQRERQSLREQY 37
>Y1C5_BACC1 (P61462) Hypothetical UPF0291 protein BCE3725
Length = 79
Score = 33.9 bits (76), Expect = 0.25
Identities = 14/34 (41%), Positives = 26/34 (76%)
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERKWLLDEF 35
+E+V+++N L +K + EGL+EEE ER+ L +++
Sbjct: 4 HELVERINFLAKKAKAEGLTEEEQRERQSLREQY 37
>YLAC_LACLA (Q9CGJ9) Hypothetical UPF0291 protein ylaC
Length = 80
Score = 33.5 bits (75), Expect = 0.32
Identities = 16/30 (53%), Positives = 22/30 (73%)
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERKWL 31
NE V+++NEL RK++ EGLSE E E+ L
Sbjct: 5 NEQVERINELARKKKAEGLSEAELEEQALL 34
>YG9A_CLOTE (P60083) Hypothetical UPF0291 protein CTC01690.1
Length = 60
Score = 32.3 bits (72), Expect = 0.72
Identities = 14/38 (36%), Positives = 27/38 (70%)
Query: 3 EVVQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLR 40
E+++++N L +K +EEGL+EEE E+ L +++ +R
Sbjct: 5 ELIERINYLYKKSKEEGLTEEEKIEQAELREKYLKNIR 42
>YR26_CLOAB (Q97FL0) Hypothetical UPF0291 protein CAC2726
Length = 55
Score = 31.6 bits (70), Expect = 1.2
Identities = 12/38 (31%), Positives = 27/38 (70%)
Query: 3 EVVQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLR 40
++++++N L +K +E+GL+EEE E+ L E+ +++
Sbjct: 5 KLIERINFLYKKSKEDGLTEEEKKEQDTLRREYIEIIK 42
>SYT_METJA (Q58597) Threonyl-tRNA synthetase (EC 6.1.1.3)
(Threonine--tRNA ligase) (ThrRS)
Length = 620
Score = 31.6 bits (70), Expect = 1.2
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Query: 111 GTLVRLDEISFKSISDADNELLTGRFGMEEVKGAVWEC--GGDKSPEPDEFNFKFIQKFW 168
G LV L + ++ D L MEE + WEC GD F+F + F+
Sbjct: 324 GELVGLKRLRCFTMPDMHTVCLNLEQAMEEFEKQFWECLKTGDDLNLSYSVIFRFTKDFF 383
Query: 169 DLLKDDFHRVLEDFWNR 185
D +D F ++ +++ N+
Sbjct: 384 DEHRDWFFKIAKEYKNK 400
>YG71_OCEIH (Q8EQM4) Hypothetical UPF0291 protein OB1671
Length = 77
Score = 31.2 bits (69), Expect = 1.6
Identities = 13/31 (41%), Positives = 22/31 (70%)
Query: 5 VQKLNELDRKEEEEGLSEEENNERKWLLDEF 35
+ ++N L +K +EEGL+ EE E+K L +E+
Sbjct: 7 LDRINVLSKKSKEEGLTTEEKAEQKKLREEY 37
>Y064_ENTFA (Q839T8) Hypothetical UPF0291 protein EF0064
Length = 74
Score = 31.2 bits (69), Expect = 1.6
Identities = 15/38 (39%), Positives = 24/38 (62%)
Query: 3 EVVQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLR 40
EV++++N L +KE E+GL+ EE R L E+ +R
Sbjct: 3 EVLKRINTLAQKEREQGLTSEEQALRVDLRQEYLRTIR 40
>OPPF_MYCGE (P47326) Oligopeptide transport ATP-binding protein oppF
Length = 848
Score = 31.2 bits (69), Expect = 1.6
Identities = 22/75 (29%), Positives = 37/75 (49%), Gaps = 4/75 (5%)
Query: 2 NEVVQKLNELDR--KEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGD 59
NE+ L L + K+E+ ++ E K LL E T L+LH+ + KW K+ +
Sbjct: 519 NELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHD--EFNHDLRKWFKKLN 576
Query: 60 QNTKYFHSLINWRKR 74
K ++ L N +K+
Sbjct: 577 FMVKKYNRLENSQKK 591
>YD41_STAAM (P60074) Hypothetical UPF0291 protein SAV1341
Length = 79
Score = 30.4 bits (67), Expect = 2.7
Identities = 20/59 (33%), Positives = 33/59 (55%), Gaps = 12/59 (20%)
Query: 5 VQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQNTK 63
++++NEL +K++E GL++EE E+ T LR Y +S K K+ +NTK
Sbjct: 8 IERINELAKKKKEVGLTQEEAKEQ--------TALR----KAYLESFRKGFKQQIENTK 54
>YC28_STAAW (P60078) Hypothetical UPF0291 protein MW1228
Length = 79
Score = 30.4 bits (67), Expect = 2.7
Identities = 20/59 (33%), Positives = 33/59 (55%), Gaps = 12/59 (20%)
Query: 5 VQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQNTK 63
++++NEL +K++E GL++EE E+ T LR Y +S K K+ +NTK
Sbjct: 8 IERINELAKKKKEVGLTQEEAKEQ--------TALR----KAYLESFRKGFKQQIENTK 54
>YB76_STAAN (P60076) Hypothetical UPF0291 protein SA1176
Length = 79
Score = 30.4 bits (67), Expect = 2.7
Identities = 20/59 (33%), Positives = 33/59 (55%), Gaps = 12/59 (20%)
Query: 5 VQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQNTK 63
++++NEL +K++E GL++EE E+ T LR Y +S K K+ +NTK
Sbjct: 8 IERINELAKKKKEVGLTQEEAKEQ--------TALR----KAYLESFRKGFKQQIENTK 54
>YA24_STAEP (Q8CSP4) Hypothetical UPF0291 protein SE1024
Length = 78
Score = 30.4 bits (67), Expect = 2.7
Identities = 15/35 (42%), Positives = 23/35 (64%), Gaps = 4/35 (11%)
Query: 5 VQKLNELDRKEEEEGLSEEENNE----RKWLLDEF 35
+ ++NEL RK++E GL+ EE E R+ L+EF
Sbjct: 8 IDRINELARKKKEHGLTNEEAKEQTKLRRQYLEEF 42
>Y585_METJA (Q58005) Hypothetical protein MJ0585
Length = 205
Score = 30.0 bits (66), Expect = 3.6
Identities = 23/92 (25%), Positives = 46/92 (50%), Gaps = 6/92 (6%)
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERKWLLDEFWTV----LRLHE*LLYQKSRSKWLKE 57
NE+ KL EL+ K + EE + K L+++ + L+ +E L Y +S W K
Sbjct: 34 NELTVKLKELESKNPNDERIEEYKEKLKQLIEKQHELKSQNLKYNETLAYIQSEEYW-KM 92
Query: 58 GDQNTKYFHSLINWRKRTN-SIMGLMIDGNWV 88
++ +Y + ++ W + I+G+++ W+
Sbjct: 93 QEEIWEYNNKVMKWFIAISILILGIILATLWI 124
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.139 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,530,138
Number of Sequences: 164201
Number of extensions: 1109461
Number of successful extensions: 3913
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 3888
Number of HSP's gapped (non-prelim): 37
length of query: 195
length of database: 59,974,054
effective HSP length: 104
effective length of query: 91
effective length of database: 42,897,150
effective search space: 3903640650
effective search space used: 3903640650
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0023.13