BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.12
(152 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TF1B_MOUSE (Q62318) Transcription intermediary factor 1-beta (TI... 34 0.11
TPR_HUMAN (P12270) Nucleoprotein TPR 31 1.2
HME1_MOUSE (P09065) Homeobox protein engrailed-1 (Mo-En-1) 31 1.2
HME1_HUMAN (Q05925) Homeobox protein engrailed-1 (Hu-En-1) 31 1.2
MACS_CHICK (P16527) Myristoylated alanine-rich C-kinase substrat... 30 1.6
SIA2_BRARE (Q7SYL3) Ubiquitin ligase SIAH2 (EC 6.3.2.-) (Seven i... 30 2.1
CCD8_RAT (P62521) Probable coiled-coil domain containing protein... 30 2.1
TMPB_TREPH (P29720) Treponemal membrane protein B precursor (Ant... 30 2.7
TF1B_HUMAN (Q13263) Transcription intermediary factor 1-beta (TI... 30 2.7
STF_LAMBD (P03764) Side tail fiber protein 30 2.7
LEA3_MAIZE (Q42376) Late embryogenesis abundant protein, group 3... 30 2.7
T10A_HUMAN (O00220) Tumor necrosis factor receptor superfamily m... 29 3.5
NRG3_MOUSE (O35181) Pro-neuregulin-3 precursor (Pro-NRG3) [Conta... 29 3.5
MST1_DROHY (Q08695) Axoneme-associated protein mst101(1) 29 3.5
HM1D_DROAN (P22544) Homeobox protein B-H1 29 3.5
CLI6_HUMAN (Q96NY7) Chloride intracellular channel 6 29 3.5
YJP1_SCHPO (Q9P6S0) Hypothetical threonine-rich protein C1742.01... 29 4.6
RX21_DROME (Q24491) RNA binding protein Rsf1 (RNA binding protei... 29 4.6
PMXB_MOUSE (O35690) Paired mesoderm homeobox protein 2B (Paired-... 29 4.6
PMXB_HUMAN (Q99453) Paired mesoderm homeobox protein 2B (Paired-... 29 4.6
>TF1B_MOUSE (Q62318) Transcription intermediary factor 1-beta
(TIF1-beta) (Tripartite motif-containing protein 28)
(KRAB-A interacting protein) (KRIP-1)
Length = 834
Score = 34.3 bits (77), Expect = 0.11
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 2/59 (3%)
Query: 2 ATTVQEATQNKRGSG--AAGTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSRLCRE 58
A T A GSG +AG E AA++ +A A + GGGE E +CRE
Sbjct: 13 AATAASAASGSPGSGEGSAGGEKRPAASSAAAASAAASSPAGGGGEAQELLEHCGVCRE 71
>TPR_HUMAN (P12270) Nucleoprotein TPR
Length = 2349
Score = 30.8 bits (68), Expect = 1.2
Identities = 17/52 (32%), Positives = 26/52 (49%), Gaps = 4/52 (7%)
Query: 7 EATQNKRGSGAA----GTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSR 54
E + G+G+A G EA+ A +G + T GGGEG R++ S+
Sbjct: 1970 EGEDSNEGTGSADGNDGYEADDAEGGDGTDPGTETEESMGGGEGNHRAADSQ 2021
>HME1_MOUSE (P09065) Homeobox protein engrailed-1 (Mo-En-1)
Length = 401
Score = 30.8 bits (68), Expect = 1.2
Identities = 19/46 (41%), Positives = 26/46 (56%), Gaps = 3/46 (6%)
Query: 4 TVQEATQNKRGSGAAGTEAEAAAAAEGMEAATA---RTAEAGGGEG 46
T A +K G+ AA A AAAAA + AA A + +++GGG G
Sbjct: 194 TAVGAGASKAGNPAAAAAAAAAAAAAAVAAAAAAASKPSDSGGGSG 239
>HME1_HUMAN (Q05925) Homeobox protein engrailed-1 (Hu-En-1)
Length = 392
Score = 30.8 bits (68), Expect = 1.2
Identities = 16/37 (43%), Positives = 21/37 (56%)
Query: 8 ATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGG 44
A +K G+ AA A AAA A AA A+ ++ GGG
Sbjct: 190 AGASKAGNPAAAAAAAAAAVAAAAAAAAAKPSDTGGG 226
>MACS_CHICK (P16527) Myristoylated alanine-rich C-kinase substrate
(MARCKS)
Length = 280
Score = 30.4 bits (67), Expect = 1.6
Identities = 20/38 (52%), Positives = 24/38 (62%), Gaps = 2/38 (5%)
Query: 10 QNKRGSG-AAGTEAEAAAAAEG-MEAATARTAEAGGGE 45
+NK+ +G A +E AAAAAEG E A A EA GGE
Sbjct: 137 KNKKEAGEGAESEGGAAAAAEGGKEEAAAAAPEAAGGE 174
>SIA2_BRARE (Q7SYL3) Ubiquitin ligase SIAH2 (EC 6.3.2.-) (Seven in
absentia homolog 2) (Siah-2)
Length = 331
Score = 30.0 bits (66), Expect = 2.1
Identities = 19/40 (47%), Positives = 22/40 (54%), Gaps = 1/40 (2%)
Query: 7 EATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEG 46
+A +K G G+ GT A AAAAA AA A A A G G
Sbjct: 18 KAGGSKHG-GSGGTTASAAAAAAAAAAAAAAAAAAAGVSG 56
>CCD8_RAT (P62521) Probable coiled-coil domain containing protein 8
homolog
Length = 643
Score = 30.0 bits (66), Expect = 2.1
Identities = 20/48 (41%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Query: 7 EATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSR 54
EAT N R AA AEAAA+ AA R AEA E ++ R
Sbjct: 333 EATANPRAEAAANPRAEAAASPRAEAAANPR-AEAAANPRAEATANPR 379
Score = 28.9 bits (63), Expect = 4.6
Identities = 18/48 (37%), Positives = 26/48 (53%), Gaps = 1/48 (2%)
Query: 7 EATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSR 54
EA +N+ G+GAA AAA++ EAA + AEA E ++ R
Sbjct: 293 EAAENQ-GAGAAAEHGVEAAASQRPEAAASPRAEAAANPRAEATANPR 339
>TMPB_TREPH (P29720) Treponemal membrane protein B precursor
(Antigen tmpB)
Length = 384
Score = 29.6 bits (65), Expect = 2.7
Identities = 28/98 (28%), Positives = 38/98 (38%), Gaps = 7/98 (7%)
Query: 6 QEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSRLCREVAECGQY 65
+EA + AA AE AAA + E AR A E +++ R+ AE Y
Sbjct: 235 EEAARKAAEEAAARKAAEEAAARKAAEEEAARI--AAEEEAARKAAEEEAARKAAEEALY 292
Query: 66 INRYKSLLPLRVFVFTAPSKRTAVKSSCHLNRCKRGAV 103
+ + +LP V T R C N K AV
Sbjct: 293 NEKGEKVLPSEYKVLTWKLDR-----ECFWNIAKNPAV 325
>TF1B_HUMAN (Q13263) Transcription intermediary factor 1-beta
(TIF1-beta) (Tripartite motif-containing protein 28)
(Nuclear corepressor KAP-1) (KRAB-associated protein 1)
(KAP-1) (KRAB-interacting protein 1) (KRIP-1)
Length = 835
Score = 29.6 bits (65), Expect = 2.7
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
Query: 14 GSGAAGTEAEA---AAAAEGMEAATARTAEAGGGEGFERSSRSRLCRE 58
G G+AG E + +AAA +A A + GG E E +CRE
Sbjct: 23 GEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRE 70
>STF_LAMBD (P03764) Side tail fiber protein
Length = 774
Score = 29.6 bits (65), Expect = 2.7
Identities = 18/41 (43%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
Query: 2 ATTVQEATQNKRGSGAAGTEAE-AAAAAEGMEAATARTAEA 41
A++ QEA+ + A TEAE +AAAAE + A A +A A
Sbjct: 163 ASSAQEASSGAEAASAKATEAEKSAAAAESSKNAAATSAGA 203
>LEA3_MAIZE (Q42376) Late embryogenesis abundant protein, group 3
(LEA)
Length = 221
Score = 29.6 bits (65), Expect = 2.7
Identities = 15/36 (41%), Positives = 19/36 (52%)
Query: 7 EATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAG 42
E T+ + TEA AA+ MEAA + AEAG
Sbjct: 116 ETTETTKQKAGETTEAARQKAADAMEAAKQKAAEAG 151
>T10A_HUMAN (O00220) Tumor necrosis factor receptor superfamily
member 10A precursor (Death receptor 4) (TNF-related
apoptosis-inducing ligand receptor 1) (TRAIL
receptor-1) (TRAIL-R1)
Length = 468
Score = 29.3 bits (64), Expect = 3.5
Identities = 14/33 (42%), Positives = 19/33 (57%)
Query: 14 GSGAAGTEAEAAAAAEGMEAATARTAEAGGGEG 46
GS A+GTEA AA ++ ++ R GGG G
Sbjct: 21 GSAASGTEAAAATPSKVWGSSAGRIEPRGGGRG 53
>NRG3_MOUSE (O35181) Pro-neuregulin-3 precursor (Pro-NRG3)
[Contains: Neuregulin-3 (NRG-3)]
Length = 713
Score = 29.3 bits (64), Expect = 3.5
Identities = 19/55 (34%), Positives = 25/55 (44%), Gaps = 5/55 (9%)
Query: 9 TQNKRGSGAAGTEAEAAAAAEGMEAATARTAEA-----GGGEGFERSSRSRLCRE 58
++ G+ G + AAA+AE AA A A A GGGEG R C +
Sbjct: 2 SEGAAGASPPGAASAAAASAEEGTAAAAAAAAAGGGPDGGGEGAAEPPRELRCSD 56
>MST1_DROHY (Q08695) Axoneme-associated protein mst101(1)
Length = 344
Score = 29.3 bits (64), Expect = 3.5
Identities = 25/107 (23%), Positives = 42/107 (38%), Gaps = 5/107 (4%)
Query: 1 MATTVQEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSRLCREVA 60
+A QEA + K+ + AA E EAA + E A A + ER+ + + E
Sbjct: 185 LAKKEQEAAEKKKCAEAAKKEKEAAEKKKCEERAKKEKEAAEKKKCEERAKKEKEAAEKK 244
Query: 61 ECGQYINRYKSLLPLRVFVFTAPSKRTA-----VKSSCHLNRCKRGA 102
+C + + K + A K+ A K + +C + A
Sbjct: 245 KCAEAAKKEKEAAEKKKCAEAAQKKKCAELAKKAKEAAEKKKCAKKA 291
>HM1D_DROAN (P22544) Homeobox protein B-H1
Length = 606
Score = 29.3 bits (64), Expect = 3.5
Identities = 16/37 (43%), Positives = 19/37 (51%)
Query: 10 QNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEG 46
Q+ + AA +AAAA AA A A AGGG G
Sbjct: 217 QHYAAAAAAAAARNSAAAAAAAAAAAASAAAAGGGGG 253
Score = 28.1 bits (61), Expect = 7.8
Identities = 15/32 (46%), Positives = 18/32 (55%)
Query: 15 SGAAGTEAEAAAAAEGMEAATARTAEAGGGEG 46
+ AA + AAAAA AA+A A GGG G
Sbjct: 224 AAAAARNSAAAAAAAAAAAASAAAAGGGGGGG 255
>CLI6_HUMAN (Q96NY7) Chloride intracellular channel 6
Length = 704
Score = 29.3 bits (64), Expect = 3.5
Identities = 17/32 (53%), Positives = 18/32 (56%), Gaps = 3/32 (9%)
Query: 16 GAAGTEAEAAAAAEGMEAA---TARTAEAGGG 44
GAAG EAE +EG E A A EAGGG
Sbjct: 32 GAAGGEAEGPEGSEGAEEAPRGAAAVKEAGGG 63
>YJP1_SCHPO (Q9P6S0) Hypothetical threonine-rich protein C1742.01 in
chromosome III precursor
Length = 1563
Score = 28.9 bits (63), Expect = 4.6
Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Query: 2 ATTVQEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAG 42
ATT E +G AGTE +A A E ++ T T AG
Sbjct: 1282 ATTATEVQTTTGATGTAGTETQATTATE-VQPTTGATGTAG 1321
Score = 28.9 bits (63), Expect = 4.6
Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Query: 2 ATTVQEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAG 42
ATT E +G AGTE +A A E ++ T T AG
Sbjct: 1200 ATTATEVQTTTGATGTAGTETQATTATE-VQPTTGATGTAG 1239
>RX21_DROME (Q24491) RNA binding protein Rsf1 (RNA binding protein
Rox21)
Length = 197
Score = 28.9 bits (63), Expect = 4.6
Identities = 18/60 (30%), Positives = 26/60 (43%), Gaps = 7/60 (11%)
Query: 12 KRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSRLCREVAECGQYINRYKS 71
+RGS + + + G A++ GGG GF R REV G+ +RY S
Sbjct: 117 QRGSSGSSSRHTERGYSSGRSGASSYNGREGGGSGFNR-------REVYGGGRDSSRYSS 169
>PMXB_MOUSE (O35690) Paired mesoderm homeobox protein 2B
(Paired-like homeobox 2B) (PHOX2B homeodomain protein)
(Neuroblastoma Phox) (NBPhox)
Length = 314
Score = 28.9 bits (63), Expect = 4.6
Identities = 16/36 (44%), Positives = 19/36 (52%)
Query: 12 KRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGF 47
K G+ AA A AAAAA AA A G G+G+
Sbjct: 238 KGGAAAAAAAAAAAAAAAAAAAAGGLAAAGGPGQGW 273
>PMXB_HUMAN (Q99453) Paired mesoderm homeobox protein 2B
(Paired-like homeobox 2B) (PHOX2B homeodomain protein)
(Neuroblastoma Phox) (NBPhox)
Length = 314
Score = 28.9 bits (63), Expect = 4.6
Identities = 16/36 (44%), Positives = 19/36 (52%)
Query: 12 KRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGF 47
K G+ AA A AAAAA AA A G G+G+
Sbjct: 238 KGGAAAAAAAAAAAAAAAAAAAAGGLAAAGGPGQGW 273
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.129 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,345,759
Number of Sequences: 164201
Number of extensions: 539999
Number of successful extensions: 3715
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 3593
Number of HSP's gapped (non-prelim): 108
length of query: 152
length of database: 59,974,054
effective HSP length: 100
effective length of query: 52
effective length of database: 43,553,954
effective search space: 2264805608
effective search space used: 2264805608
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0023.12