BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.1
(416 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PIF1_YEAST (P07271) DNA repair and recombination protein PIF1, m... 50 1e-05
PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1, m... 49 2e-05
YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergeni... 44 6e-04
EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 35 0.46
KHL1_MOUSE (Q9JI74) Kelch-like protein 1 33 1.4
KHL1_HUMAN (Q9NR64) Kelch-like protein 1 32 2.3
IFH1_YEAST (P39520) IFH1 protein (RRP3 protein) 32 3.0
HELI_EHV1B (P28934) Probable helicase 31 5.1
EX5A_BUCBP (Q89AB2) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 31 5.1
SYM_CORGL (Q8NRZ4) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 31 6.7
GATD_PYRAB (Q9V0T9) Glutamyl-tRNA(Gln) amidotransferase subunit ... 31 6.7
EX5A_HAEIN (P45158) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 31 6.7
GATD_PYRHO (O59132) Glutamyl-tRNA(Gln) amidotransferase subunit ... 30 8.8
>PIF1_YEAST (P07271) DNA repair and recombination protein PIF1,
mitochondrial precursor
Length = 857
Score = 49.7 bits (117), Expect = 1e-05
Identities = 27/59 (45%), Positives = 41/59 (68%), Gaps = 1/59 (1%)
Query: 338 SRRQFPIIMCFVMTINKSEGQSLSHVGLYLPKPVFTHGQLYVALSRVKSRKGLKIFIVD 396
SR Q P+++ + ++I+KS+GQ+L V + L + VF GQ YVALSR SR+GL++ D
Sbjct: 690 SRVQLPLMLAWSLSIHKSQGQTLPKVKVDLRR-VFEKGQAYVALSRAVSREGLQVLNFD 747
>PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1,
mitochondrial precursor
Length = 805
Score = 49.3 bits (116), Expect = 2e-05
Identities = 24/55 (43%), Positives = 40/55 (72%), Gaps = 1/55 (1%)
Query: 338 SRRQFPIIMCFVMTINKSEGQSLSHVGLYLPKPVFTHGQLYVALSRVKSRKGLKI 392
SR Q P+I+ + ++I+K++GQ+L V + L + VF GQ YVALSR +++GL++
Sbjct: 707 SRSQIPLILAYAISIHKAQGQTLDRVKVDLGR-VFEKGQAYVALSRATTQEGLQV 760
>YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergenic
region
Length = 723
Score = 44.3 bits (103), Expect = 6e-04
Identities = 21/65 (32%), Positives = 42/65 (64%), Gaps = 1/65 (1%)
Query: 339 RRQFPIIMCFVMTINKSEGQSLSHVGLYLPKPVFTHGQLYVALSRVKSRKGLKIFIVDDR 398
R Q P+++C+ ++I+K++GQ++ + + L + +F GQ+YVALSR + L++ D
Sbjct: 646 RTQIPLMLCWALSIHKAQGQTIQRLKVDL-RRIFEAGQVYVALSRAVTMDTLQVLNFDPG 704
Query: 399 GVVSN 403
+ +N
Sbjct: 705 KIRTN 709
>EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5) (Exodeoxyribonuclease V 67 kDa polypeptide)
Length = 608
Score = 34.7 bits (78), Expect = 0.46
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 3/46 (6%)
Query: 348 FVMTINKSEGQSLSHVGLYLPK---PVFTHGQLYVALSRVKSRKGL 390
+ MT++KS+G H L LP PV T +Y A++R + R L
Sbjct: 534 WAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRRLSL 579
>KHL1_MOUSE (Q9JI74) Kelch-like protein 1
Length = 751
Score = 33.1 bits (74), Expect = 1.4
Identities = 25/92 (27%), Positives = 45/92 (48%), Gaps = 15/92 (16%)
Query: 125 MRLQHAASSSSSMEIKELADWLLQVGDEIVKPINDDDSNIE------------IPSDLLV 172
M++ H+ + + ME+ ++LL +E+ K + DD N+ + D+
Sbjct: 333 MKVAHSYTMENIMEVIRNQEFLLLPAEELHKLLASDDVNVPDEETIFHALMMWVKYDMQR 392
Query: 173 RESDNPILEL---VDFAYPEIVANLENHAYFE 201
R SD +L + P+I+A+LENHA F+
Sbjct: 393 RCSDLSMLLAFIRLPLLPPQILADLENHALFK 424
>KHL1_HUMAN (Q9NR64) Kelch-like protein 1
Length = 748
Score = 32.3 bits (72), Expect = 2.3
Identities = 24/92 (26%), Positives = 45/92 (48%), Gaps = 15/92 (16%)
Query: 125 MRLQHAASSSSSMEIKELADWLLQVGDEIVKPINDDDSNIE------------IPSDLLV 172
M++ H+ + + ME+ ++LL +E+ K + DD N+ + D+
Sbjct: 330 MKVAHSYTMENIMEVIRNQEFLLLPAEELHKLLASDDVNVPDEETIFHALMMWVKYDMQS 389
Query: 173 RESDNPILEL---VDFAYPEIVANLENHAYFE 201
R +D +L + P+I+A+LENHA F+
Sbjct: 390 RCNDLSMLLAFIRLPLLPPQILADLENHALFK 421
>IFH1_YEAST (P39520) IFH1 protein (RRP3 protein)
Length = 1085
Score = 32.0 bits (71), Expect = 3.0
Identities = 36/130 (27%), Positives = 57/130 (43%), Gaps = 16/130 (12%)
Query: 157 INDDDSNIEIPSDLLVRESDNPILELVDFAYPEIVANLENHAYFE-QRALLAHTLESVEE 215
IN++DS+ EI +DL E D PILE + IV+ L+N ++ + VE+
Sbjct: 331 INNEDSHGEIGTDLETGEDDLPILEEEE---QNIVSELQNDDELSFDGSIHEEGSDPVED 387
Query: 216 VNNFMLSMIPGEETKYLSYDTPCRSDEDSDIDA*WFTSEFLNDVKCSGI------PNHRI 269
N L +E Y D DE+ +I + F F D K + + ++
Sbjct: 388 AENKFLQNEYNQENGYDEED-----DEEDEIMS-DFDMPFYEDPKFANLYYYGDGSEPKL 441
Query: 270 VLKVEVPIML 279
L +P+ML
Sbjct: 442 SLSTSLPLML 451
>HELI_EHV1B (P28934) Probable helicase
Length = 881
Score = 31.2 bits (69), Expect = 5.1
Identities = 16/43 (37%), Positives = 25/43 (57%)
Query: 350 MTINKSEGQSLSHVGLYLPKPVFTHGQLYVALSRVKSRKGLKI 392
MTI +S+G SL V + P+ +YVA+SR S + L++
Sbjct: 807 MTIARSQGLSLDKVAICFPRNNLRINSVYVAMSRTVSSRFLRM 849
>EX5A_BUCBP (Q89AB2) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5)
Length = 618
Score = 31.2 bits (69), Expect = 5.1
Identities = 20/65 (30%), Positives = 36/65 (54%), Gaps = 6/65 (9%)
Query: 348 FVMTINKSEGQSLSHVGLYLP---KPVFTHGQLYVALSRVKSRKGLKIFIVDDRGVVSNC 404
+ MT++KS+G S V L LP + T +Y A++R S+K L I+ D+ + +
Sbjct: 544 WTMTVHKSQGSEFSEVVLILPTIMTSILTKELIYTAVTR--SKKKLTIY-SDENIFIKSL 600
Query: 405 TRNVV 409
+N++
Sbjct: 601 KKNII 605
>SYM_CORGL (Q8NRZ4) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 610
Score = 30.8 bits (68), Expect = 6.7
Identities = 18/66 (27%), Positives = 32/66 (48%), Gaps = 3/66 (4%)
Query: 165 EIPSDLLVRESDNPILELVDFAYPEIVANLENHAYFEQRALLAHTLESVEEVNNFMLSMI 224
E+P + ESD IL+L A+ + ANL+ + +A ++ + V E N ++
Sbjct: 421 EVPVPGALEESDKKILDLATAAFESVAANLDQSKF---KAGISEIMHVVGEANAYIAEQE 477
Query: 225 PGEETK 230
P + K
Sbjct: 478 PWKLAK 483
>GATD_PYRAB (Q9V0T9) Glutamyl-tRNA(Gln) amidotransferase subunit D
(EC 6.3.5.-) (Glu-ADT subunit D)
Length = 438
Score = 30.8 bits (68), Expect = 6.7
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Query: 194 LENHAYFEQRALLAHTLESVEEVNNFMLSMIPGEETKYLSYDTPCR 239
L AY + +L HT +++EEV ML+ GE T Y +DT R
Sbjct: 394 LPETAYVKLMWVLGHT-QNLEEVRKMMLTNYAGEITPYTRFDTYLR 438
>EX5A_HAEIN (P45158) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5)
Length = 640
Score = 30.8 bits (68), Expect = 6.7
Identities = 17/49 (34%), Positives = 27/49 (54%), Gaps = 5/49 (10%)
Query: 348 FVMTINKSEGQSLSHVGLYLP---KPVFTHGQLYVALSRVKSRKGLKIF 393
F+MTI+KS+G H + LP PV + ++ ++R K K L +F
Sbjct: 566 FMMTIHKSQGSEFKHTVMVLPTEVNPVLSRELVFTGVTRAK--KELTVF 612
>GATD_PYRHO (O59132) Glutamyl-tRNA(Gln) amidotransferase subunit D
(EC 6.3.5.-) (Glu-ADT subunit D)
Length = 438
Score = 30.4 bits (67), Expect = 8.8
Identities = 18/46 (39%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Query: 194 LENHAYFEQRALLAHTLESVEEVNNFMLSMIPGEETKYLSYDTPCR 239
L AY + +L HT + +EEV ML+ GE T Y +DT R
Sbjct: 394 LPETAYVKLMWVLGHT-QDLEEVRRMMLTNYAGEITPYTRFDTYLR 438
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.141 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,102,005
Number of Sequences: 164201
Number of extensions: 1776724
Number of successful extensions: 5286
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 5275
Number of HSP's gapped (non-prelim): 17
length of query: 416
length of database: 59,974,054
effective HSP length: 113
effective length of query: 303
effective length of database: 41,419,341
effective search space: 12550060323
effective search space used: 12550060323
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 67 (30.4 bits)
Lotus: description of TM0023.1