BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0021.5
(210 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AD19_MOUSE (O35674) ADAM 19 precursor (EC 3.4.24.-) (A disintegr... 36 0.057
CHKA_HUMAN (P35790) Choline kinase alpha (EC 2.7.1.32) (CK) (CHE... 33 0.48
GOA3_MOUSE (P55937) Golgi autoantigen, golgin subfamily A member... 32 1.1
FOG1_HUMAN (Q8IX07) Zinc finger protein ZFPM1 (Zinc finger prote... 32 1.1
S3A1_HUMAN (Q15459) Splicing factor 3 subunit 1 (Spliceosome ass... 32 1.4
G150_MOUSE (Q8BL07) Probable G protein-coupled receptor 150 (G p... 32 1.4
DRPL_RAT (P54258) Atrophin-1 (Dentatorubral-pallidoluysian atrop... 32 1.4
ZYX_MOUSE (Q62523) Zyxin 31 1.8
YG51_YEAST (P50089) Hypothetical 89.2 kDa protein in YHB1-PFK1 i... 31 1.8
VGF_HUMAN (O15240) Neurosecretory protein VGF precursor 31 1.8
PIP1_HUMAN (Q9H074) Polyadenylate-binding protein-interacting pr... 31 1.8
P5PA_MOUSE (P59644) Phosphatidylinositol 4,5-bisphosphate 5-phos... 31 1.8
LRP3_RAT (O88204) Low-density lipoprotein receptor-related prote... 31 1.8
PRP_MEDSA (Q40358) Repetitive proline-rich cell wall protein pre... 31 2.4
FMN1_MOUSE (Q05860) Formin 1 isoforms I/II/III (Limb deformity p... 31 2.4
FM14_MOUSE (Q05859) Formin 1 isoform IV (Limb deformity protein) 31 2.4
AD19_HUMAN (Q9H013) ADAM 19 precursor (EC 3.4.24.-) (A disintegr... 31 2.4
PRGR_RABIT (P06186) Progesterone receptor (PR) 30 3.1
PC15_MOUSE (Q99PJ1) Protocadherin 15 precursor 30 3.1
P5PA_RAT (Q9JMC1) Phosphatidylinositol 4,5-bisphosphate 5-phosph... 30 3.1
>AD19_MOUSE (O35674) ADAM 19 precursor (EC 3.4.24.-) (A disintegrin
and metalloproteinase domain 19) (Meltrin beta)
Length = 920
Score = 36.2 bits (82), Expect = 0.057
Identities = 18/54 (33%), Positives = 25/54 (45%), Gaps = 10/54 (18%)
Query: 39 RKAATRKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGE 92
RK + R+PPP R P P P CLL + PR P + +P+ G+
Sbjct: 829 RKESARRPPPSR----------PMPPAPNCLLSQDFSRPRPPQKALPANPVPGQ 872
>CHKA_HUMAN (P35790) Choline kinase alpha (EC 2.7.1.32) (CK)
(CHETK-alpha)
Length = 457
Score = 33.1 bits (74), Expect = 0.48
Identities = 18/39 (46%), Positives = 19/39 (48%), Gaps = 3/39 (7%)
Query: 47 PPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYL 85
PPP L LP PL QP P PP + PR R YL
Sbjct: 58 PPPPPLPLPLPLPQPPPPQPPA---DEQPEPRTRRRAYL 93
>GOA3_MOUSE (P55937) Golgi autoantigen, golgin subfamily A member 3
(Golgin-160) (Male-enhanced antigen-2) (MEA-2)
Length = 1447
Score = 32.0 bits (71), Expect = 1.1
Identities = 20/74 (27%), Positives = 35/74 (47%), Gaps = 3/74 (4%)
Query: 17 VQTMKNKISTAVVLTRCYRDDTRKAATRKPPPP---RTLLLPFPLFQPRAPLPPCLLHEA 73
V +KN + T + + + D R+ A +K P + P+ P P+P LL E
Sbjct: 1309 VSELKNNMKTLLQQNQQLKLDLRRGAAKKKEPKGESNSSSPATPIKIPDCPVPASLLEEL 1368
Query: 74 MKPPRNPSRRYLQS 87
++PP S+ L++
Sbjct: 1369 LRPPPAVSKEPLKN 1382
>FOG1_HUMAN (Q8IX07) Zinc finger protein ZFPM1 (Zinc finger protein
multitype 1) (Friend of GATA protein 1) (Friend of
GATA-1) (FOG-1)
Length = 1004
Score = 32.0 bits (71), Expect = 1.1
Identities = 21/60 (35%), Positives = 27/60 (45%), Gaps = 4/60 (6%)
Query: 49 PRTLLLPFPLFQPRAPLP-PCLLHEAMKPPRNP---SRRYLQSPISGEPLTIWRNQHHHC 104
PR L P P P P P P EA+ PP P S + +Q+P G P + H +C
Sbjct: 915 PRDGLGPEPQEPPPGPPPSPAAAPEAVPPPPAPPSYSDKGVQTPSKGTPAPLPNGNHRYC 974
>S3A1_HUMAN (Q15459) Splicing factor 3 subunit 1 (Spliceosome
associated protein 114) (SAP 114) (SF3a120)
Length = 793
Score = 31.6 bits (70), Expect = 1.4
Identities = 23/74 (31%), Positives = 32/74 (43%), Gaps = 13/74 (17%)
Query: 37 DTRKAATRKPPPPRTLLLPFPLFQPRAPLPPCLL------HEAMKP--PRNPSRRYLQSP 88
D + + PPPP T P+ P P P ++ +A KP P YL SP
Sbjct: 360 DDEEEGQKVPPPPET-----PMPPPLPPTPDQVIVRKDYDPKASKPLPPAPAPDEYLVSP 414
Query: 89 ISGEPLTIWRNQHH 102
I+GE + + Q H
Sbjct: 415 ITGEKIPASKMQEH 428
>G150_MOUSE (Q8BL07) Probable G protein-coupled receptor 150 (G
protein-coupled receptor PGR11)
Length = 427
Score = 31.6 bits (70), Expect = 1.4
Identities = 13/30 (43%), Positives = 15/30 (49%)
Query: 141 RWPRNHHHHALHGERNKTLYSFSSPATLPP 170
RWP H+HHA ERN+ P PP
Sbjct: 390 RWPHPHYHHARREERNQGCLRPPPPRPRPP 419
>DRPL_RAT (P54258) Atrophin-1 (Dentatorubral-pallidoluysian atrophy
protein)
Length = 1183
Score = 31.6 bits (70), Expect = 1.4
Identities = 18/49 (36%), Positives = 24/49 (48%), Gaps = 4/49 (8%)
Query: 46 PPPPRTL-LLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEP 93
PPPP ++ P F+P +PP H M+P P+ R Q P G P
Sbjct: 168 PPPPDSIPRQPESGFEPHPSVPPTGYHAPMEP---PTSRLFQGPPPGAP 213
>ZYX_MOUSE (Q62523) Zyxin
Length = 564
Score = 31.2 bits (69), Expect = 1.8
Identities = 16/42 (38%), Positives = 21/42 (49%), Gaps = 4/42 (9%)
Query: 46 PPPPRTLLLPFPLFQPR----APLPPCLLHEAMKPPRNPSRR 83
PPP T +P P +P APLPP + +PP P R+
Sbjct: 175 PPPVATPFVPKPSTKPAPGGTAPLPPWKTPSSSQPPPQPQRK 216
>YG51_YEAST (P50089) Hypothetical 89.2 kDa protein in YHB1-PFK1
intergenic region
Length = 785
Score = 31.2 bits (69), Expect = 1.8
Identities = 23/67 (34%), Positives = 29/67 (42%), Gaps = 4/67 (5%)
Query: 102 HHCTPLTVRNKTPQEIFRFAALRRRASA----LQLAISPSFGLRWPRNHHHHALHGERNK 157
H T L N+ P+ F +R+ SA + S S G R +HHHH H R K
Sbjct: 682 HIKTALKWDNEDPKMKFTSHPMRKTKSAGLSPRTSSTSSSSGQRKAHHHHHHHNHVSRTK 741
Query: 158 TLYSFSS 164
T S S
Sbjct: 742 TTESTKS 748
>VGF_HUMAN (O15240) Neurosecretory protein VGF precursor
Length = 616
Score = 31.2 bits (69), Expect = 1.8
Identities = 31/122 (25%), Positives = 46/122 (37%), Gaps = 27/122 (22%)
Query: 35 RDDTRKAATRKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPL 94
R+ +KA PPPR P + P+ P PP + + P + P E
Sbjct: 481 RNRKKKAPPEPVPPPRAAPAPTHVRSPQPPPPPPSARDEL-----PDWNEVLPPWDREED 535
Query: 95 TIWRNQHHHCTPLTVRNKTPQEIFRFAALRRRASALQLAISPSFGLRWPRNHHHHALHGE 154
++ +H P +R +T Q +ALRRR H+HHAL
Sbjct: 536 EVYPPGPYHPFPNYIRPRTLQPP---SALRRR-------------------HYHHALPPS 573
Query: 155 RN 156
R+
Sbjct: 574 RH 575
>PIP1_HUMAN (Q9H074) Polyadenylate-binding protein-interacting
protein 1 (Poly(A) binding protein-interacting protein
1) (PABP-interacting protein 1) (PAIP-1)
Length = 479
Score = 31.2 bits (69), Expect = 1.8
Identities = 18/43 (41%), Positives = 21/43 (47%), Gaps = 2/43 (4%)
Query: 42 ATRKPPPPRT--LLLPFPLFQPRAPLPPCLLHEAMKPPRNPSR 82
A +PP P+ L P PL QPR PP E P+ PSR
Sbjct: 41 ARHQPPQPKAPGFLQPPPLRQPRTTPPPGAQCEVPASPQRPSR 83
>P5PA_MOUSE (P59644) Phosphatidylinositol 4,5-bisphosphate
5-phosphatase A (EC 3.1.3.56)
Length = 1003
Score = 31.2 bits (69), Expect = 1.8
Identities = 20/59 (33%), Positives = 25/59 (41%), Gaps = 16/59 (27%)
Query: 46 PPPPRTLLLPF----------------PLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSP 88
PPPP+TL L P F+P P + KPPR+PSR +SP
Sbjct: 299 PPPPQTLPLDVSPGLPESGTRSPGLLSPTFRPGIPSSQTVPPPLRKPPRSPSRSPSRSP 357
>LRP3_RAT (O88204) Low-density lipoprotein receptor-related protein
3 precursor (rLRp105)
Length = 770
Score = 31.2 bits (69), Expect = 1.8
Identities = 24/79 (30%), Positives = 33/79 (41%), Gaps = 19/79 (24%)
Query: 46 PPPPRTLLLPF-------------PLFQPRAPLP----PCLLHEAMKPPRNPSRRYLQSP 88
PPPP L P PL AP+ PCL + P+ P+ +Q P
Sbjct: 682 PPPPLNLRDPEYRPEDKERKACVDPLEDSPAPVDTPPEPCLAQDPH--PQTPTASGIQDP 739
Query: 89 ISGEPLTIWRNQHHHCTPL 107
S EPL + R+ C+P+
Sbjct: 740 HSAEPLGVCRSPPPTCSPI 758
>PRP_MEDSA (Q40358) Repetitive proline-rich cell wall protein
precursor (MSPRP)
Length = 236
Score = 30.8 bits (68), Expect = 2.4
Identities = 19/75 (25%), Positives = 29/75 (38%)
Query: 3 SFFTFLFMCLILEIVQTMKNKISTAVVLTRCYRDDTRKAATRKPPPPRTLLLPFPLFQPR 62
S F L + + I Q + N V Y+ K KPP + + P+++P
Sbjct: 4 SNFLVLLLFALFVIPQGLANYDKPPVYQPPVYKPPVEKPPVYKPPVEKPPVYKPPVYKPP 63
Query: 63 APLPPCLLHEAMKPP 77
PP +KPP
Sbjct: 64 VEKPPVYKPPVVKPP 78
>FMN1_MOUSE (Q05860) Formin 1 isoforms I/II/III (Limb deformity
protein)
Length = 1468
Score = 30.8 bits (68), Expect = 2.4
Identities = 23/72 (31%), Positives = 31/72 (42%), Gaps = 3/72 (4%)
Query: 12 LILEIVQTMKNKISTAVVLTRCYRDDTRKAATRK---PPPPRTLLLPFPLFQPRAPLPPC 68
L + + Q +K S + R+ T ++ +K P PP LP PL P PLPP
Sbjct: 834 LTISLTQLSPSKDSKDIHAPFQTREGTSSSSQQKISPPAPPTPPPLPPPLIPPPPPLPPG 893
Query: 69 LLHEAMKPPRNP 80
L PP P
Sbjct: 894 LGPLPPAPPIPP 905
>FM14_MOUSE (Q05859) Formin 1 isoform IV (Limb deformity protein)
Length = 1206
Score = 30.8 bits (68), Expect = 2.4
Identities = 23/72 (31%), Positives = 31/72 (42%), Gaps = 3/72 (4%)
Query: 12 LILEIVQTMKNKISTAVVLTRCYRDDTRKAATRK---PPPPRTLLLPFPLFQPRAPLPPC 68
L + + Q +K S + R+ T ++ +K P PP LP PL P PLPP
Sbjct: 608 LTISLTQLSPSKDSKDIHAPFQTREGTSSSSQQKISPPAPPTPPPLPPPLIPPPPPLPPG 667
Query: 69 LLHEAMKPPRNP 80
L PP P
Sbjct: 668 LGPLPPAPPIPP 679
>AD19_HUMAN (Q9H013) ADAM 19 precursor (EC 3.4.24.-) (A disintegrin
and metalloproteinase domain 19) (Meltrin beta)
(Metalloprotease and disintegrin dentritic antigen
marker) (MADDAM) (FKSG34 protein)
Length = 956
Score = 30.8 bits (68), Expect = 2.4
Identities = 15/53 (28%), Positives = 24/53 (44%), Gaps = 10/53 (18%)
Query: 39 RKAATRKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISG 91
R ++R+PPP R P P P C++ + PR P + +P+ G
Sbjct: 828 RTESSRRPPPSR----------PIPPAPNCIVSQDFSRPRPPQKALPANPVPG 870
>PRGR_RABIT (P06186) Progesterone receptor (PR)
Length = 930
Score = 30.4 bits (67), Expect = 3.1
Identities = 22/63 (34%), Positives = 29/63 (45%), Gaps = 12/63 (19%)
Query: 37 DTRKAATRKPPPPRTLLLP------FPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPIS 90
+ +AA PRT L+ FP FQ AP PP L PPR PS R ++ ++
Sbjct: 388 EEEEAAEAAARSPRTYLVAGANPAAFPDFQLAAPPPPSL------PPRVPSSRPGEAAVA 441
Query: 91 GEP 93
P
Sbjct: 442 ASP 444
>PC15_MOUSE (Q99PJ1) Protocadherin 15 precursor
Length = 1943
Score = 30.4 bits (67), Expect = 3.1
Identities = 18/48 (37%), Positives = 22/48 (45%), Gaps = 5/48 (10%)
Query: 46 PPPPRTLLLPFPLFQP---RAPLPPCLLHEAMKPPRNPSRRYLQSPIS 90
PPPP+ + P+ P PLPP L PPR P+ R P S
Sbjct: 1775 PPPPQLVTFSLPISTPPTSSLPLPPPL--SLPPPPRPPAPRLFPQPPS 1820
>P5PA_RAT (Q9JMC1) Phosphatidylinositol 4,5-bisphosphate
5-phosphatase A (EC 3.1.3.56) (Proline-rich inositol
polyphosphate 5-phosphatase)
Length = 1001
Score = 30.4 bits (67), Expect = 3.1
Identities = 20/59 (33%), Positives = 25/59 (41%), Gaps = 16/59 (27%)
Query: 46 PPPPRTLLLPF----------------PLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSP 88
PPPP+TL L P F+P P + KPPR+PSR +SP
Sbjct: 299 PPPPQTLPLDVSSGLPESGTRSPGLLSPTFRPGIPSNQTVPPPLPKPPRSPSRSPSRSP 357
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.139 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,553,528
Number of Sequences: 164201
Number of extensions: 1105871
Number of successful extensions: 5215
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 36
Number of HSP's that attempted gapping in prelim test: 5073
Number of HSP's gapped (non-prelim): 134
length of query: 210
length of database: 59,974,054
effective HSP length: 105
effective length of query: 105
effective length of database: 42,732,949
effective search space: 4486959645
effective search space used: 4486959645
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0021.5