BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0019b.9
(138 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
INVO_RAT (P48998) Involucrin 33 0.24
CAUP_DROME (P54269) Homeobox protein caupolican 31 0.70
EML4_HUMAN (Q9HC35) Echinoderm microtubule-associated protein-li... 30 1.2
SOC6_MOUSE (Q9JLY0) Suppressor of cytokine signaling 6 (Suppress... 30 1.6
CLA4_YEAST (P48562) Serine/threonine-protein kinase CLA4 (EC 2.7... 30 1.6
TF20_HUMAN (Q9UGU0) Transcription factor 20 (Stromelysin 1 PDGF-... 30 2.0
NPBL_MOUSE (Q6KCD5) Nipped-B-like protein (Delangin homolog) (SC... 30 2.0
LSB6_YEAST (P42951) Phosphatidylinositol 4-kinase LSB6 (EC 2.7.1... 30 2.0
LAP1_RAT (P70587) LAP1 protein (Densin-180) 30 2.0
LAP1_MOUSE (Q80TE7) LAP1 protein (Densin-180) 30 2.0
CAD5_PIG (O02840) Vascular endothelial-cadherin precursor (VE-ca... 30 2.0
PKN2_CLOAB (Q97IC2) Probable serine/threonine protein kinase CAC... 29 2.7
PIA1_MOUSE (O88907) Protein inhibitor of activated STAT protein ... 29 2.7
PIA1_HUMAN (O75925) Protein inhibitor of activated STAT protein ... 29 3.5
IPL1_YEAST (P38991) Serine/threonine-protein kinase IPL1 (EC 2.7... 29 3.5
GALT_BACHD (Q9KDV2) Galactose-1-phosphate uridylyltransferase (E... 29 3.5
NFH_RAT (P16884) Neurofilament triplet H protein (200 kDa neurof... 28 4.5
YJ03_YEAST (P47104) Hypothetical 154.9 kDa protein in CPR7-PET19... 28 5.9
MSS4_YEAST (P38994) Probable phosphatidylinositol-4-phosphate 5-... 28 5.9
LRP5_HUMAN (O75197) Low-density lipoprotein receptor-related pro... 28 5.9
>INVO_RAT (P48998) Involucrin
Length = 568
Score = 32.7 bits (73), Expect = 0.24
Identities = 24/69 (34%), Positives = 38/69 (54%), Gaps = 13/69 (18%)
Query: 26 KTHTKPNKNQI----QQEIHYA------KAPQNRHTPYDLHIGEPQESNAPEPTRNLKYK 75
K H K + ++ QQE+H K Q+ H P +LH+G+ QES+ P+ T + K K
Sbjct: 386 KQHEKSPEPELHLGKQQELHEPDMTEDQKEKQSLHEP-ELHLGKQQESHEPDMTEDQKEK 444
Query: 76 FLKSLCKPK 84
+SL +P+
Sbjct: 445 --QSLYEPE 451
Score = 30.4 bits (67), Expect = 1.2
Identities = 15/63 (23%), Positives = 30/63 (46%)
Query: 22 EGKLKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLKSLC 81
E +L + + ++E+H K Q +LH+G+ Q + EP +L + + C
Sbjct: 239 EPELCLRKQQQQESHERELHLGKQQQQESHEPELHLGKQQHQESHEPELHLGKQQHQESC 298
Query: 82 KPK 84
+P+
Sbjct: 299 EPE 301
>CAUP_DROME (P54269) Homeobox protein caupolican
Length = 693
Score = 31.2 bits (69), Expect = 0.70
Identities = 13/52 (25%), Positives = 25/52 (48%)
Query: 9 NKFTSSLETRQVPEGKLKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEP 60
N + S +Q+P L+ H + Q+QQ+ + P + H + + +G P
Sbjct: 484 NFYPPSSADQQLPHQPLQQHQQQQLQQLQQQQQHHHHPHHHHPHHSMELGSP 535
>EML4_HUMAN (Q9HC35) Echinoderm microtubule-associated protein-like
4 (EMAP-4) (Restrictedly overexpressed
proliferation-associated protein) (Ropp 120)
Length = 981
Score = 30.4 bits (67), Expect = 1.2
Identities = 14/56 (25%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Query: 18 RQVPEGKLKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLK 73
++ P+G+ + + + N +I + +PQ P +H P+ NA PT+++K
Sbjct: 119 KEKPQGQREKKEESHSNDQSPQIRASPSPQPSSQPLQIHRQTPESKNA-TPTKSIK 173
>SOC6_MOUSE (Q9JLY0) Suppressor of cytokine signaling 6 (Suppressor
of cytokine signaling 4) (SOCS-4) (Cytokine-inducible
SH2 protein 4) (CIS-4)
Length = 533
Score = 30.0 bits (66), Expect = 1.6
Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 2/60 (3%)
Query: 77 LKSLCKPKPITVSSL-THGSSPTPYSILRVFHNSSSPHLQLAQRSIFIEDSPSP-NSVKR 134
LK + P+PI +SL +H SPTP+ + + +++A ++ SP P N V++
Sbjct: 109 LKDVRAPRPIRSTSLRSHHYSPTPWPLRPTSSEETCIKMEIASEALVHAASPGPVNGVRK 168
>CLA4_YEAST (P48562) Serine/threonine-protein kinase CLA4 (EC
2.7.1.37)
Length = 842
Score = 30.0 bits (66), Expect = 1.6
Identities = 22/92 (23%), Positives = 36/92 (38%), Gaps = 2/92 (2%)
Query: 19 QVPEGKLKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLK 78
Q P G + H ++ +QQ+ + + +H Y H P S +P P+ Y+
Sbjct: 356 QFPRGPM--HPNNSQRSLQQQQQQQQQQKQQHQQYPYHHQGPSPSPSPSPSPLNPYRPHH 413
Query: 79 SLCKPKPITVSSLTHGSSPTPYSILRVFHNSS 110
++ P S S +I R NSS
Sbjct: 414 NMINPYSKQPQSPLSSQSTQNQAIPRYAQNSS 445
>TF20_HUMAN (Q9UGU0) Transcription factor 20 (Stromelysin 1
PDGF-responsive element-binding protein) (SPRE-binding
protein) (Nuclear factor SPBP) (AR1)
Length = 1960
Score = 29.6 bits (65), Expect = 2.0
Identities = 21/57 (36%), Positives = 27/57 (46%), Gaps = 6/57 (10%)
Query: 19 QVPEGKLKTHTKPNKNQIQQEIHYAKA-PQNRHTPYDLHIGEPQESNAPEPTRNLKY 74
Q+PEG KP K + ++E A P+ R T + I EPQ EP LKY
Sbjct: 1565 QIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQ-----EPEIKLKY 1616
>NPBL_MOUSE (Q6KCD5) Nipped-B-like protein (Delangin homolog) (SCC2
homolog)
Length = 2798
Score = 29.6 bits (65), Expect = 2.0
Identities = 20/88 (22%), Positives = 35/88 (39%), Gaps = 1/88 (1%)
Query: 13 SSLETR-QVPEGKLKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRN 71
S LET+ + P +LK + Q E + QN + P D + +++N
Sbjct: 638 SKLETKTETPTEELKQNENKTTESKQSESAVVEPKQNENRPCDTKPNDNKQNNTRSENTK 697
Query: 72 LKYKFLKSLCKPKPITVSSLTHGSSPTP 99
+ + K + +P T + G TP
Sbjct: 698 ARPETPKQKAESRPETPKQKSEGRPETP 725
>LSB6_YEAST (P42951) Phosphatidylinositol 4-kinase LSB6 (EC
2.7.1.67) (PI4-kinase) (PtdIns-4-kinase)
Length = 607
Score = 29.6 bits (65), Expect = 2.0
Identities = 15/43 (34%), Positives = 18/43 (40%)
Query: 27 THTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPT 69
TH P Q A+APQ H Y + P ES P+ T
Sbjct: 42 THASPGVGSNAQNSDIAEAPQVFHPSYQSLVNVPSESPRPDQT 84
>LAP1_RAT (P70587) LAP1 protein (Densin-180)
Length = 1495
Score = 29.6 bits (65), Expect = 2.0
Identities = 22/76 (28%), Positives = 31/76 (39%), Gaps = 6/76 (7%)
Query: 26 KTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLKSLCKPKP 85
K T P + + + + K P TP G P E + P P R+L F + C+P
Sbjct: 866 KLETTPTTSPLPERKDHMKEPTE--TPGPFSPGVPWEYHDPTPNRSLGNVFSQIHCRPDS 923
Query: 86 ----ITVSSLTHGSSP 97
I +S T SP
Sbjct: 924 SKGVIAISKSTERLSP 939
>LAP1_MOUSE (Q80TE7) LAP1 protein (Densin-180)
Length = 1490
Score = 29.6 bits (65), Expect = 2.0
Identities = 22/76 (28%), Positives = 31/76 (39%), Gaps = 6/76 (7%)
Query: 26 KTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLKSLCKPKP 85
K T P + + + + K P TP G P E + P P R+L F + C+P
Sbjct: 861 KLETTPTTSPLPERKDHMKEPTE--TPGPFSPGVPWEYHDPTPNRSLGNVFSQIHCRPDS 918
Query: 86 ----ITVSSLTHGSSP 97
I +S T SP
Sbjct: 919 SKGVIAISKSTERLSP 934
>CAD5_PIG (O02840) Vascular endothelial-cadherin precursor
(VE-cadherin) (Cadherin-5)
Length = 782
Score = 29.6 bits (65), Expect = 2.0
Identities = 28/97 (28%), Positives = 46/97 (46%), Gaps = 15/97 (15%)
Query: 46 PQNRHTPYDLHIGEPQESNA--PEPTRNLKYKFLKSLCKP-KPITVSSLTHGS----SPT 98
PQNR T Y + GE +++ P+PTRN + + KP KP+ + S +
Sbjct: 285 PQNRKTKYSIVQGEYRDTFTIEPDPTRN------EGIIKPMKPLDYERIQQYSFTIEATD 338
Query: 99 PYSILRVFHNSSSPHLQLAQRSIFIEDSPSPNSVKRP 135
P LR +S+ + +A+ I + D P + K+P
Sbjct: 339 PTIDLRYLSGTSTKN--IARVIINVTDVDEPPNFKQP 373
>PKN2_CLOAB (Q97IC2) Probable serine/threonine protein kinase
CAC1728 (EC 2.7.1.37)
Length = 657
Score = 29.3 bits (64), Expect = 2.7
Identities = 16/57 (28%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Query: 17 TRQVPEGKLKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLK 73
TR + +++ + PN + +Y P N+ P + EPQE N EP +K
Sbjct: 281 TRVMDPAQIQNESNPNNKLDNDDTYYNGEPYNKEQPQE----EPQEEN-EEPKNKIK 332
>PIA1_MOUSE (O88907) Protein inhibitor of activated STAT protein 1
(DEAD/H box binding protein 1)
Length = 651
Score = 29.3 bits (64), Expect = 2.7
Identities = 28/98 (28%), Positives = 36/98 (36%), Gaps = 19/98 (19%)
Query: 25 LKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLKSLCKPK 84
LK P +E++ + PQ TP DL I S P PT +
Sbjct: 45 LKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMP-PTLS------------- 90
Query: 85 PITVSSLTHGSSPT-----PYSILRVFHNSSSPHLQLA 117
P T+ LT+ P P S+L H PHL A
Sbjct: 91 PSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSA 128
>PIA1_HUMAN (O75925) Protein inhibitor of activated STAT protein 1
(Gu binding protein) (GBP) (RNA helicase II binding
protein) (DEAD/H box-binding protein 1)
Length = 651
Score = 28.9 bits (63), Expect = 3.5
Identities = 27/98 (27%), Positives = 36/98 (36%), Gaps = 19/98 (19%)
Query: 25 LKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLKSLCKPK 84
LK P +E++ + PQ TP DL I P ++P P
Sbjct: 45 LKAGCSPAVQMKIKELYRRRFPQKIMTPADLSI--PNVHSSPMP------------ATLS 90
Query: 85 PITVSSLTHGSSPT-----PYSILRVFHNSSSPHLQLA 117
P T+ LT+ P P S+L H PHL A
Sbjct: 91 PSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSA 128
>IPL1_YEAST (P38991) Serine/threonine-protein kinase IPL1 (EC
2.7.1.37)
Length = 367
Score = 28.9 bits (63), Expect = 3.5
Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 4/77 (5%)
Query: 26 KTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLKSLCKPKP 85
KT T+PN ++I + + +PQ R+ P +E P N K+ ++S P P
Sbjct: 19 KTTTRPNTSRINKPWRISHSPQQRN-PNSKIPSPVREKLNRLPVNNKKFLDMESSKIPSP 77
Query: 86 I---TVSSLTHGSSPTP 99
I T S + H + P
Sbjct: 78 IRKATSSKMIHENKKLP 94
>GALT_BACHD (Q9KDV2) Galactose-1-phosphate uridylyltransferase (EC
2.7.7.12) (Gal-1-P uridylyltransferase)
(UDP-glucose--hexose-1-phosphate uridylyltransferase)
Length = 508
Score = 28.9 bits (63), Expect = 3.5
Identities = 22/90 (24%), Positives = 37/90 (40%), Gaps = 2/90 (2%)
Query: 23 GKLKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLKSLCK 82
G+L+ +T ++ + ++ A Q T + E E PE + Y F K +
Sbjct: 72 GRLEANTVTYRDLLDTKLMGALLGQPSETIRTFY--ERYEQQGPEEATKVFYDFSKQVHY 129
Query: 83 PKPITVSSLTHGSSPTPYSILRVFHNSSSP 112
+ ++ H S TPY L + N S P
Sbjct: 130 IRTDRIAKNEHWFSETPYGQLEITINLSKP 159
>NFH_RAT (P16884) Neurofilament triplet H protein (200 kDa
neurofilament protein) (Neurofilament heavy polypeptide)
(NF-H) (Fragment)
Length = 831
Score = 28.5 bits (62), Expect = 4.5
Identities = 21/78 (26%), Positives = 32/78 (40%)
Query: 13 SSLETRQVPEGKLKTHTKPNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNL 72
S E + E K K + + + AK+P+ +P I P E +PE ++
Sbjct: 509 SPAEAKSPVEVKSPEKAKSPVKEGAKSLAEAKSPEKAKSPVKEEIKPPAEVKSPEKAKSP 568
Query: 73 KYKFLKSLCKPKPITVSS 90
K KS K K + V S
Sbjct: 569 MRKEAKSPEKAKTLDVKS 586
>YJ03_YEAST (P47104) Hypothetical 154.9 kDa protein in CPR7-PET191
intergenic region
Length = 1357
Score = 28.1 bits (61), Expect = 5.9
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 2/55 (3%)
Query: 58 GEPQESNAPEPTRNLKYKFLKSLCKPKPITVS--SLTHGSSPTPYSILRVFHNSS 110
G P S+ P L L +P P + S SL SS P SIL F + S
Sbjct: 1243 GSPSASDIESPNPKLPNSLLDDFLQPPPNSTSSNSLAQSSSSAPRSILDEFVSPS 1297
>MSS4_YEAST (P38994) Probable phosphatidylinositol-4-phosphate
5-kinase MSS4 (EC 2.7.1.68)
(1-phosphatidylinositol-4-phosphate kinase) (PIP5K)
(PtdIns(4)P-5-kinase) (Diphosphoinositide kinase)
Length = 779
Score = 28.1 bits (61), Expect = 5.9
Identities = 29/115 (25%), Positives = 37/115 (31%), Gaps = 33/115 (28%)
Query: 31 PNKNQIQQEIHYAKAPQNRHTPYDLHIGEPQESNAPEPTRNLKYKFLKSLCKPKPITVSS 90
PN N H K +N HT Y H ESN P
Sbjct: 45 PNSNSNPDSNHRIKKDRNNHTSY--HSSSNSESNMESP---------------------R 81
Query: 91 LTHGSSPTPYSILRVFHNSSSPHLQLAQRSIFIE----------DSPSPNSVKRP 135
L+ G S TP SI + ++ L SI I+ D PN++ P
Sbjct: 82 LSDGESSTPTSIEELNPTINNSRLVKRNYSISIDPLHDNSNNNTDDDHPNTITSP 136
>LRP5_HUMAN (O75197) Low-density lipoprotein receptor-related protein
5 precursor
Length = 1615
Score = 28.1 bits (61), Expect = 5.9
Identities = 14/47 (29%), Positives = 19/47 (39%)
Query: 53 YDLHIGEPQESNAPEPTRNLKYKFLKSLCKPKPITVSSLTHGSSPTP 99
Y L + + P PT + +Y + C P P T S H P P
Sbjct: 1562 YYLDLNSDSDPYPPPPTPHSQYLSAEDSCPPSPATERSYFHLFPPPP 1608
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.311 0.128 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,783,915
Number of Sequences: 164201
Number of extensions: 766248
Number of successful extensions: 1283
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1267
Number of HSP's gapped (non-prelim): 29
length of query: 138
length of database: 59,974,054
effective HSP length: 99
effective length of query: 39
effective length of database: 43,718,155
effective search space: 1705008045
effective search space used: 1705008045
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0019b.9