BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0010.19
(295 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YQIK_ECOLI (P77306) Hypothetical protein yqiK 33 0.65
YG36_METMA (Q8PWF3) Hypothetical glycosyl transferase MM1636 (EC... 31 3.2
VP4_ROTHL (P21284) Outer capsid protein VP4 (Hemagglutinin) (Out... 31 4.2
CN16_ECOLI (P08331) 2',3'-cyclic-nucleotide 2'-phosphodiesterase... 30 5.5
CHB1_DESVH (P62636) Chemotaxis response regulator protein-glutam... 30 5.5
YOT5_CAEEL (P34651) Hypothetical protein ZK632.5 in chromosome III 30 7.1
VP4_ROTBK (P36308) Outer capsid protein VP4 (Hemagglutinin) (Out... 30 7.1
SKT5_YEAST (P34226) SKT5 protein 30 7.1
ENPL_HORVU (P36183) Endoplasmin homolog precursor (GRP94 homolog) 30 9.3
CV19_HUMAN (Q13769) Protein C22orf19 (NF2/meningioma region prot... 30 9.3
>YQIK_ECOLI (P77306) Hypothetical protein yqiK
Length = 553
Score = 33.5 bits (75), Expect = 0.65
Identities = 26/118 (22%), Positives = 55/118 (46%), Gaps = 18/118 (15%)
Query: 8 SLSSQSSIRTSIPGRPNIAITNSASSSERISNRRRTMSAVEARSSLILALASQANS---- 63
+++++S ++ R N+A+ + S+ + + R+T A A+ ++A A A +
Sbjct: 336 AIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVE 395
Query: 64 LTQR-------------LVVDVATETARYLFPKRLESRTLEEALMAVPDLET-VKFKV 107
LT R +V++A T + + R L +A+ + D +T +KFK+
Sbjct: 396 LTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKL 453
>YG36_METMA (Q8PWF3) Hypothetical glycosyl transferase MM1636 (EC
2.-.-.-)
Length = 379
Score = 31.2 bits (69), Expect = 3.2
Identities = 51/214 (23%), Positives = 80/214 (36%), Gaps = 53/214 (24%)
Query: 108 LSRRDNYEIREVEPYFVAEATMPGKSG-FDFSGASQSFNVLAEYLFGKNTLK-------- 158
L + Y I E+ +E + GK+G FD SG+ ++ A L G LK
Sbjct: 43 LVEKTGYRIHEIP----SEIKLVGKAGGFDLSGSIEATLKNARILGGPKVLKLIKDFKPD 98
Query: 159 ------------EKMEMTTPVYTSKNQSD--------GVKM----DMTTPVYTAKMEDQD 194
M + PVY NQS+ GV + D+T Y E D
Sbjct: 99 VVVSDSYYLGTLAAMLLNIPVYLIINQSNMEDFFKNRGVPIRLLGDLTKKFYREVFEKTD 158
Query: 195 KWTM-SFILP---------------SKYGANLPLPKDSSVRIKEIPRKIVAVVSFSGFVN 238
K + + LP K + PL K+ +++IP K +VS G
Sbjct: 159 KIIIPDYPLPYTVCRKNLNFSPGLWEKLYYSGPLVKEKYEEVEQIPLKKPHIVSLIGGFG 218
Query: 239 DEEVKQRELKLREALKSDGQFKIKKGTSVEVAQY 272
E R++ AL S + + G S++ +++
Sbjct: 219 YREPIFRKVLTTAALDSGINYTLISGPSLDPSKF 252
>VP4_ROTHL (P21284) Outer capsid protein VP4 (Hemagglutinin) (Outer
layer protein VP4) [Contains: Outer capsid protein VP8;
Outer capsid protein VP5]
Length = 775
Score = 30.8 bits (68), Expect = 4.2
Identities = 29/117 (24%), Positives = 53/117 (44%), Gaps = 6/117 (5%)
Query: 12 QSSIRTSIPGRPNIAITNSASSSERISNRRRTMSAVEARSSLILALASQANSLTQRLVVD 71
+S + TSI N +++++ASS+ R ++ R +S + + AS++ S LV D
Sbjct: 553 KSKLATSISEMTN-SLSDAASSASRSASVRSNLSVISNWTD-----ASKSTSNITDLVND 606
Query: 72 VATETARYLFPKRLESRTLEEALMAVPDLETVKFKVLSRRDNYEIREVEPYFVAEAT 128
V+T+T+ RL+ + M+ D+ K + P V EA+
Sbjct: 607 VSTQTSTISKKLRLKEMITQTEGMSFDDISAAVLKTKIDMSTQIGKNTLPDIVTEAS 663
>CN16_ECOLI (P08331) 2',3'-cyclic-nucleotide 2'-phosphodiesterase
precursor (EC 3.1.4.16)
Length = 647
Score = 30.4 bits (67), Expect = 5.5
Identities = 22/83 (26%), Positives = 36/83 (42%), Gaps = 5/83 (6%)
Query: 63 SLTQRLVVDVATETARYLFPKRLESRTLEEALMAVPDLETVKFKVLSRRDNYEIREVE-- 120
+L+ ++ V+ TET R P+ E ++A L +KV++ Y + E+
Sbjct: 191 NLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENSVYYLSEIPGV 250
Query: 121 ---PYFVAEATMPGKSGFDFSGA 140
+ A A PGK D GA
Sbjct: 251 NAIMFGHAHAVFPGKDFADIEGA 273
>CHB1_DESVH (P62636) Chemotaxis response regulator protein-glutamate
methylesterase group 1 operon (EC 3.1.1.61)
Length = 357
Score = 30.4 bits (67), Expect = 5.5
Identities = 20/85 (23%), Positives = 35/85 (40%)
Query: 82 PKRLESRTLEEALMAVPDLETVKFKVLSRRDNYEIREVEPYFVAEATMPGKSGFDFSGAS 141
P+ R LEE+ + + D + R + R+V P A+A +PG + F +
Sbjct: 109 PRLGTKRFLEESSIRICDAVKAAARARLHRLGHRTRDVAPKLTADAMLPGPTNFTRVQTT 168
Query: 142 QSFNVLAEYLFGKNTLKEKMEMTTP 166
+ V+ G L+ +E P
Sbjct: 169 EKVVVVGASTGGTEALQVFLEAMPP 193
>YOT5_CAEEL (P34651) Hypothetical protein ZK632.5 in chromosome III
Length = 824
Score = 30.0 bits (66), Expect = 7.1
Identities = 24/82 (29%), Positives = 40/82 (48%), Gaps = 6/82 (7%)
Query: 203 PSKYGANLPLPKDSSVRIKEIPRKIVA--VVSFSGFVNDEEVKQRELKLREALKSDGQFK 260
PS + PL +SS+ I E VA ++ + FVND++ K ++L + KSD
Sbjct: 429 PSIWQRLNPLQMESSLCIDEDLANRVADLIIGYQFFVNDKKSKSQKLDEKTFAKSD---- 484
Query: 261 IKKGTSVEVAQYNPPFTLPFQR 282
I+ +E+ P F + Q+
Sbjct: 485 IQDAEELELFAKTPEFEIIIQK 506
>VP4_ROTBK (P36308) Outer capsid protein VP4 (Hemagglutinin) (Outer
layer protein VP4) [Contains: Outer capsid protein VP8;
Outer capsid protein VP5]
Length = 772
Score = 30.0 bits (66), Expect = 7.1
Identities = 33/135 (24%), Positives = 54/135 (39%), Gaps = 19/135 (14%)
Query: 26 AITNSASSSERISNRRRTMSAVEARSSLILALASQANSLTQRLVVDVATETARYLFPKRL 85
AIT++ASS R S R SAV + + S T +V AT A+ R+
Sbjct: 566 AITDAASSISRSSTLRSANSAVSVWTDI-----SDIVDSTDNVVAATATAAAKKF---RV 617
Query: 86 ESRTLEEALMAVPDLETVKFKVLSRRDNYEIREVEPYFVAEAT-----------MPGKSG 134
+ T E ++ D+ K + N E+ P + EA+ + G+
Sbjct: 618 KEFTTEFDGVSFDDISAAVVKTKMNKFNVVDEEILPRIITEASEKFIPNRAYRLIDGEKV 677
Query: 135 FDFSGASQSFNVLAE 149
++ + +SF L E
Sbjct: 678 YEVTTEGKSFAYLTE 692
>SKT5_YEAST (P34226) SKT5 protein
Length = 696
Score = 30.0 bits (66), Expect = 7.1
Identities = 28/100 (28%), Positives = 46/100 (46%), Gaps = 9/100 (9%)
Query: 6 NASLSSQSSIRTSIPGRPNIAITNSASSSERISNRRRTMSAVEARSSLILALASQANSLT 65
N SLSS S T+ P + TNS++S + ++RT S + L+ + + T
Sbjct: 109 NTSLSSLGSTPTNSPSPGALRQTNSSTSLTKEQIKKRTRSVDLSHMYLLNGSSDTQLTAT 168
Query: 66 QRLVVDVATE-TARYLFPKRLESRTLEEALMAVPDLETVK 104
V D++ + +RYL K S VP L+T++
Sbjct: 169 NESVADLSHQMISRYLGGKNNTS--------LVPRLKTIE 200
>ENPL_HORVU (P36183) Endoplasmin homolog precursor (GRP94 homolog)
Length = 809
Score = 29.6 bits (65), Expect = 9.3
Identities = 22/87 (25%), Positives = 39/87 (44%), Gaps = 14/87 (16%)
Query: 172 NQSDGVKMDMTTPVYTAKMEDQDKWTMSFILPSKYGANLPLPKDSSVRIKEIPRKIVAVV 231
+ S+G+K TA ME G NLP PKD + I +K + +
Sbjct: 725 SDSEGLKQTARLVYQTALMES--------------GFNLPDPKDFASSIYRSVQKSLDLS 770
Query: 232 SFSGFVNDEEVKQRELKLREALKSDGQ 258
+ +EEV++ E++ +E+ K + +
Sbjct: 771 PDAAVEEEEEVEEPEVEEKESAKQEAE 797
>CV19_HUMAN (Q13769) Protein C22orf19 (NF2/meningioma region protein
pK1.3) (Placental protein 39.2)
Length = 683
Score = 29.6 bits (65), Expect = 9.3
Identities = 23/75 (30%), Positives = 34/75 (44%), Gaps = 2/75 (2%)
Query: 31 ASSSERISNRRRTMSAVEARSSLILALASQANSLTQRLVVDVATETARYLFPKRLESRTL 90
A S S+ TM ++ R LAL Q SL +V T +YLFP ++ SR +
Sbjct: 463 ADHSLSASHMETTMKLLKTRVQSRLALHKQFASLEHGIVP--VTSDCQYLFPAKVVSRLV 520
Query: 91 EEALMAVPDLETVKF 105
+ +A D + F
Sbjct: 521 KWVTIAHEDYMELHF 535
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.314 0.129 0.347
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,880,151
Number of Sequences: 164201
Number of extensions: 1156699
Number of successful extensions: 2582
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2579
Number of HSP's gapped (non-prelim): 12
length of query: 295
length of database: 59,974,054
effective HSP length: 109
effective length of query: 186
effective length of database: 42,076,145
effective search space: 7826162970
effective search space used: 7826162970
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0010.19