BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0004a.8
(1041 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ARP2_PLAFA (P13824) Clustered-asparagine-rich protein (Fragment) 37 0.28
PEPL_HUMAN (O60437) Periplakin (195 kDa cornified envelope precu... 36 0.62
BPA1_HUMAN (Q03001) Bullous pemphigoid antigen 1 isoforms 1/2/3/... 36 0.62
YAP6_YEAST (Q03935) AP-1 like transcription factor YAP6 35 0.80
PEPL_MOUSE (Q9R269) Periplakin 35 0.80
PTGA_BUCAI (Q9WXI7) PTS system, glucose-specific IIA component (... 35 1.1
SCP1_HUMAN (Q15431) Synaptonemal complex protein 1 (SCP-1 protein) 35 1.4
C48A_ARATH (P54609) Cell division control protein 48 homolog A (... 35 1.4
MFP1_LYCES (P93203) MAR binding filament-like protein 1 34 1.8
ILVC_LEPIN (Q8EYH2) Ketol-acid reductoisomerase (EC 1.1.1.86) (A... 34 1.8
ILVC_LEPIC (Q72M00) Ketol-acid reductoisomerase (EC 1.1.1.86) (A... 34 1.8
I4G3_MOUSE (Q80XI3) Eukaryotic translation initiation factor 4 g... 34 1.8
Y623_CHLPN (Q9Z7T1) Protein CPn0623/CP0124/CPj0623/CpB0649 34 2.3
FX32_HUMAN (Q969P5) F-box only protein 32 (Muscle atrophy F-box ... 34 2.3
RRPL_BUNYW (P20470) RNA-directed RNA polymerase (EC 2.7.7.48) (L... 33 3.1
T2FA_HUMAN (P35269) Transcription initiation factor IIF, alpha s... 33 4.0
RRN3_YEAST (P36070) RNA polymerase I specific transcription init... 33 4.0
MFP1_TOBAC (Q9M7J4) MAR binding filament-like protein 1-1 33 4.0
IFH1_YEAST (P39520) IFH1 protein (RRP3 protein) 33 4.0
EVPL_MOUSE (Q9D952) Envoplakin (p210) (210 kDa cornified envelop... 33 4.0
>ARP2_PLAFA (P13824) Clustered-asparagine-rich protein (Fragment)
Length = 451
Score = 37.0 bits (84), Expect = 0.28
Identities = 21/98 (21%), Positives = 39/98 (39%)
Query: 169 SNSKKSVRENNRSSKTATMNRMLKNKLQNKRNNLQDKRKLLMQRKAGESNQHVTRNQSLK 228
+NS + N +++ MNR +KNK N NN + +M +NQ + N L
Sbjct: 171 NNSSNNNNNNQTNTQNNFMNRNMKNKNMNNNNNNNNSNNNMMMNMNFNNNQQMNNNPMLN 230
Query: 229 EKCELSLDSEISEEGVDRISMLIDDEELELRELQERTN 266
+ + ++ ++ + D E+ L N
Sbjct: 231 QNNFMLNNNNNYNNNAKNVNDMYRDGEMSPNHLNNNNN 268
>PEPL_HUMAN (O60437) Periplakin (195 kDa cornified envelope precursor)
(190 kDa paraneoplastic pemphigus antigen)
Length = 1756
Score = 35.8 bits (81), Expect = 0.62
Identities = 28/90 (31%), Positives = 47/90 (52%), Gaps = 4/90 (4%)
Query: 178 NNRSSKTATMNRMLKNKLQNKRNNLQ-DKRKLLMQRKAGESNQHVTRNQSLKEKCELSLD 236
N++SSK R +KLQ +R NLQ + R+L + + RN ++ + + D
Sbjct: 1551 NSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINMAATETRDLRNMTVADS-GTNHD 1609
Query: 237 SEI--SEEGVDRISMLIDDEELELRELQER 264
S + E +D + L D++LE+ ELQ+R
Sbjct: 1610 SRLWSLERELDDLKRLSKDKDLEIDELQKR 1639
>BPA1_HUMAN (Q03001) Bullous pemphigoid antigen 1 isoforms 1/2/3/4/5/8
(230 kDa bullous pemphigoid antigen) (BPA)
(Hemidesmosomal plaque protein) (Dystonia musculorum
protein) (Dystonin) (Fragment)
Length = 3214
Score = 35.8 bits (81), Expect = 0.62
Identities = 22/65 (33%), Positives = 35/65 (53%), Gaps = 5/65 (7%)
Query: 170 NSKKSVRENNRSSKTATMNRMLKNKLQN--KRNNLQDKRKLLMQRKAGESNQHVTRNQSL 227
N+ K R N K+ MLK K++ ++NN + KL+MQR ES V Q++
Sbjct: 2116 NNDKIQRLNEELEKSNECAEMLKQKVEELTRQNN---ETKLMMQRIQAESENIVLEKQTI 2172
Query: 228 KEKCE 232
+++CE
Sbjct: 2173 QQRCE 2177
>YAP6_YEAST (Q03935) AP-1 like transcription factor YAP6
Length = 383
Score = 35.4 bits (80), Expect = 0.80
Identities = 19/67 (28%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
Query: 162 DREVHLISNSKKSVRENNRSSKTATMNRMLKNKLQNKRNNLQDKRKLLMQRKAGESNQHV 221
D++ LIS+S K++R R+++ T + + + + NL+ K K+ A E+N
Sbjct: 209 DKDTQLISSSGKTLRNTRRAAQNRTAQKAFRQRKEKYIKNLEQKSKIFDDLLA-ENNNFK 267
Query: 222 TRNQSLK 228
+ N SL+
Sbjct: 268 SLNDSLR 274
>PEPL_MOUSE (Q9R269) Periplakin
Length = 1755
Score = 35.4 bits (80), Expect = 0.80
Identities = 27/93 (29%), Positives = 44/93 (47%), Gaps = 10/93 (10%)
Query: 178 NNRSSKTATMNRMLKNKLQNKRNNLQDKRKLLMQRKAGESNQHVTRNQSLKEKCELSLDS 237
N++SSK R +KLQ +R NLQ L +R E T + LK + +
Sbjct: 1550 NSKSSKELDFLREENHKLQLERQNLQ----LETRRLQSEIEMAATETRDLKNITTIDSGT 1605
Query: 238 EIS------EEGVDRISMLIDDEELELRELQER 264
++ E+ +D + + D++LE+ ELQ R
Sbjct: 1606 HLNSRLWSLEKELDDLKKMSKDKDLEIDELQRR 1638
>PTGA_BUCAI (Q9WXI7) PTS system, glucose-specific IIA component
(EIIA-Glc) (Glucose-permease IIA component)
(Phosphotransferase enzyme II, A component) (EC
2.7.1.69) (EIII-Glc)
Length = 161
Score = 35.0 bits (79), Expect = 1.1
Identities = 25/75 (33%), Positives = 39/75 (51%), Gaps = 6/75 (8%)
Query: 413 LNLLINYGISPGTLKGELFKILSERGNNGCKVSDLAKATQIAELNLASTTEELESLICST 472
+ L I++GI LKGE FK + N K+ D +I L+L E+ ES++
Sbjct: 78 VELFIHFGIDTVKLKGEGFK-KKAKDNQKVKIGD-----EIIILDLEFIKEKAESILTPV 131
Query: 473 LSSDITLFEKISSSA 487
+ S+I F+KI S+
Sbjct: 132 VISNIENFKKIKKSS 146
>SCP1_HUMAN (Q15431) Synaptonemal complex protein 1 (SCP-1 protein)
Length = 976
Score = 34.7 bits (78), Expect = 1.4
Identities = 24/105 (22%), Positives = 51/105 (47%), Gaps = 7/105 (6%)
Query: 171 SKKSVRENNRSSKTATMNRMLKNKLQNKRNNLQDKRKLLMQRKAGESNQHVTRN------ 224
+K S+ + +T+ M LKN+ ++ NN + + ++L Q + + + RN
Sbjct: 521 NKLSLENKELTQETSDMTLELKNQQEDINNNKKQEERMLKQIENLQETETQLRNELEYVR 580
Query: 225 QSLKEK-CELSLDSEISEEGVDRISMLIDDEELELRELQERTNLL 268
+ LK+K E+ + SEE + + ++++ + ELQ+ L
Sbjct: 581 EELKQKRDEVKCKLDKSEENCNNLRKQVENKNKYIEELQQENKAL 625
>C48A_ARATH (P54609) Cell division control protein 48 homolog A
(AtCDC48a)
Length = 809
Score = 34.7 bits (78), Expect = 1.4
Identities = 55/250 (22%), Positives = 97/250 (38%), Gaps = 27/250 (10%)
Query: 424 GTLKGELFKILSERGNNGCKVSDLAKATQIAELNLASTTEELESLICSTLSSDITLFEKI 483
G + L K E N + + + IA T E+E I S L +TL + +
Sbjct: 283 GESESNLRKAFEEAEKNAPSIIFIDEIDSIAPKR-EKTNGEVERRIVSQL---LTLMDGL 338
Query: 484 SSSAYRLRMSTVTKDDDECQSDMEDYGSVDDELDDSDTCSCGDDFESGSVDSNIRKLKRV 543
S A+ + M T + + +G D E+D V I +L+ +
Sbjct: 339 KSRAHVIVMGA-TNRPNSIDPALRRFGRFDREIDIG-------------VPDEIGRLEVL 384
Query: 544 KSHKTKSAKLTV---FNEIDESHPGEVWL-LGLMESEYSDLNIEEKLNALAALTDLLSSG 599
+ H TK+ KL I + G V L + +E + I EK++ + DL
Sbjct: 385 RIH-TKNMKLAEDVDLERISKDTHGYVGADLAALCTEAALQCIREKMDVI----DLEDDS 439
Query: 600 SSIRMKDPVKVTADYDSSIQLRGSGAKIKRSVVKKPVPFWNQFGQMQRVKEGHLNSHPCP 659
+ + + VT ++ + + + ++ +VV+ P WN G ++ VK + P
Sbjct: 440 IDAEILNSMAVTNEHFHTALGNSNPSALRETVVEVPNVSWNDIGGLENVKRELQETVQYP 499
Query: 660 VDSSSLMSKF 669
V+ KF
Sbjct: 500 VEHPEKFEKF 509
>MFP1_LYCES (P93203) MAR binding filament-like protein 1
Length = 697
Score = 34.3 bits (77), Expect = 1.8
Identities = 28/101 (27%), Positives = 54/101 (52%), Gaps = 8/101 (7%)
Query: 162 DREVHLISNSKKSVRENNRSSKTATMNRMLKNKLQNKRNNLQDKRKLL--MQRKAGESNQ 219
+ E+H +S+ +V EN S +T +N + K ++ RN L+ ++ ++ ++ +
Sbjct: 501 NEEMHTMSDELVAVSENRDSLQTELVN--VYKKREHTRNELKQEKTIVRTLEEELKFLES 558
Query: 220 HVTRNQSLKEKCELSLDSEISEEGVDRI--SMLIDDEELEL 258
+TR + L++ E L E + E +D I ++L EELEL
Sbjct: 559 QITREKELRKSLEDEL--EKATESLDEINRNVLALAEELEL 597
>ILVC_LEPIN (Q8EYH2) Ketol-acid reductoisomerase (EC 1.1.1.86)
(Acetohydroxy-acid isomeroreductase)
(Alpha-keto-beta-hydroxylacil reductoisomerase)
Length = 374
Score = 34.3 bits (77), Expect = 1.8
Identities = 32/123 (26%), Positives = 55/123 (44%), Gaps = 20/123 (16%)
Query: 584 EKLNALAALTDLLSSGSSIRMKDPVKVTADYDSSIQLRGSGAKIKRSVVKKPVPFWNQFG 643
E L+ + +TDL+ G RM+ + TA+Y + SG ++ VK
Sbjct: 268 ECLHEVKLITDLIYEGGLARMRFSISDTAEYGDYV----SGPRVIDPGVK---------- 313
Query: 644 QMQRVKE--GHLNSHPCPVDSSSLMSKFRSHEPSFEKGKGSTDSHPIQSVYLGSDRRYNR 701
QR+KE + +++ M++ ++ P+F+ + SHPI+SV G R
Sbjct: 314 --QRMKEVLNDIQKDKGAKFATNWMAETKAGYPNFKNMRDKNASHPIESV--GKKLRSMM 369
Query: 702 YWL 704
WL
Sbjct: 370 KWL 372
>ILVC_LEPIC (Q72M00) Ketol-acid reductoisomerase (EC 1.1.1.86)
(Acetohydroxy-acid isomeroreductase)
(Alpha-keto-beta-hydroxylacil reductoisomerase)
Length = 374
Score = 34.3 bits (77), Expect = 1.8
Identities = 32/123 (26%), Positives = 55/123 (44%), Gaps = 20/123 (16%)
Query: 584 EKLNALAALTDLLSSGSSIRMKDPVKVTADYDSSIQLRGSGAKIKRSVVKKPVPFWNQFG 643
E L+ + +TDL+ G RM+ + TA+Y + SG ++ VK
Sbjct: 268 ECLHEVKLITDLIYEGGLARMRFSISDTAEYGDYV----SGPRVIDPGVK---------- 313
Query: 644 QMQRVKE--GHLNSHPCPVDSSSLMSKFRSHEPSFEKGKGSTDSHPIQSVYLGSDRRYNR 701
QR+KE + +++ M++ ++ P+F+ + SHPI+SV G R
Sbjct: 314 --QRMKEVLNDIQKDKGAKFATNWMAETKAGYPNFKNMRDKNASHPIESV--GKKLRSMM 369
Query: 702 YWL 704
WL
Sbjct: 370 KWL 372
>I4G3_MOUSE (Q80XI3) Eukaryotic translation initiation factor 4 gamma
3 (eIF-4-gamma 3) (eIF-4G 3) (eIF4G 3) (eIF-4-gamma II)
(eIF4GII)
Length = 1579
Score = 34.3 bits (77), Expect = 1.8
Identities = 23/72 (31%), Positives = 38/72 (51%), Gaps = 5/72 (6%)
Query: 211 QRKAGESNQHVTRNQSLKEKCELSLDSE--ISEEGVDRISMLIDDEELELRELQERTNLL 268
+R + E+++ TR + E C + + +SEE V+R S I DE L + + +E T
Sbjct: 1181 ERASTEADRSKTRELAKSEMCAVPAPDKPALSEEEVERKSKSIIDEFLHINDFKEATQ-- 1238
Query: 269 ICSDHLAASGML 280
C + L+A G L
Sbjct: 1239 -CIEELSAQGPL 1249
>Y623_CHLPN (Q9Z7T1) Protein CPn0623/CP0124/CPj0623/CpB0649
Length = 277
Score = 33.9 bits (76), Expect = 2.3
Identities = 28/121 (23%), Positives = 53/121 (43%), Gaps = 4/121 (3%)
Query: 499 DDECQSDMEDYGSVDDELDDSDTCSCGDDFESGSVDSNIRKLKRVKSHKTKSAKLTVFNE 558
D++ +D + G+ + D G +KL R ++HK + E
Sbjct: 109 DEKPLADSPEEGTEPTSPSKKEKKEARKDSFKGEKWKEKKKLSRRRNHKEIAEVTGASQE 168
Query: 559 IDESHPGEVWLLGLMESEYSDLNIEEKLNALAALTDLLSSGSSIRMKDPVKVTADYDSSI 618
I ++ E+W ES+ +++ ++K + L L+S S + DPV +AD + S+
Sbjct: 169 ILDTVKEELW----EESQENEIVEQKKFSLLPPPAKLISEVISQTVVDPVVTSADLNESL 224
Query: 619 Q 619
Q
Sbjct: 225 Q 225
>FX32_HUMAN (Q969P5) F-box only protein 32 (Muscle atrophy F-box
protein) (MAFbx) (Atrogin-1)
Length = 355
Score = 33.9 bits (76), Expect = 2.3
Identities = 16/55 (29%), Positives = 24/55 (43%)
Query: 832 EEQVQKWIRVQEYDSWIWNSFYLDLNVVKYGRRSYLDSLARCKSCHDLYWRDERH 886
E Q++K + + + W Y L + Y D+L CK CH L W+ H
Sbjct: 276 ERQIRKRLILSDKGQLDWKKMYFKLVRCYPRKEQYGDTLQLCKHCHILSWKGTDH 330
>RRPL_BUNYW (P20470) RNA-directed RNA polymerase (EC 2.7.7.48) (L
protein)
Length = 2238
Score = 33.5 bits (75), Expect = 3.1
Identities = 16/38 (42%), Positives = 24/38 (63%)
Query: 228 KEKCELSLDSEISEEGVDRISMLIDDEELELRELQERT 265
KE+C+L+ D ISE G ++ +L E E+R + ERT
Sbjct: 980 KERCKLNTDEMISEPGDSKLKILEKKAEEEIRYIVERT 1017
>T2FA_HUMAN (P35269) Transcription initiation factor IIF, alpha
subunit (EC 2.7.1.37) (TFIIF-alpha) (Transcription
initiation factor RAP74) (General transcription factor
IIF polypeptide 1 74 kDa subunit protein)
Length = 517
Score = 33.1 bits (74), Expect = 4.0
Identities = 20/72 (27%), Positives = 36/72 (49%), Gaps = 3/72 (4%)
Query: 36 GRIIQQREKANVPSSAKKNQKRKCLQDLGTVLRNDGPSIGREFDYLPSGHKSYNSTCRED 95
GR+ + ++KA + +K +K+K D +DG G+E DY+ G +S+ E
Sbjct: 229 GRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGS---SSSQEEP 285
Query: 96 QQPAKRSKVSKG 107
+ AK + +G
Sbjct: 286 ESKAKAPQQEEG 297
>RRN3_YEAST (P36070) RNA polymerase I specific transcription
initiation factor RRN3
Length = 627
Score = 33.1 bits (74), Expect = 4.0
Identities = 25/77 (32%), Positives = 36/77 (46%), Gaps = 10/77 (12%)
Query: 470 CSTLSSDI--TLFEKISSSAYRLR-----MSTVTKDDDECQSDMEDYGSVDDELDDSDTC 522
CS L I L EKI S L+ + DDD + D+ED +DD+ D D
Sbjct: 222 CSELGFQIWSLLIEKIISIDVELQNELDELDDDVDDDDLEEVDLEDDDDLDDDSGDDDDE 281
Query: 523 SCG---DDFESGSVDSN 536
+CG ++ SG+ D +
Sbjct: 282 NCGNSNEELRSGAADGS 298
>MFP1_TOBAC (Q9M7J4) MAR binding filament-like protein 1-1
Length = 722
Score = 33.1 bits (74), Expect = 4.0
Identities = 33/145 (22%), Positives = 70/145 (47%), Gaps = 22/145 (15%)
Query: 162 DREVHLISNSKKSVRENNRSSKTATMNRMLKNKLQNKRNNLQD--------KRKLLMQRK 213
D+E L S + K + + K ++M + +++L N + +++ +R+L ++R+
Sbjct: 268 DKEASLQSTTSKLAEKESEVDKLSSMYQESQDQLMNLTSEIKELKVEVQKRERELELKRE 327
Query: 214 AGESNQHVTRNQSLKEKCELSLDSE-----------ISEEGVDRISMLIDDEELELRELQ 262
+ E N +V N L E+ E + + ISE+ V + L+ ++E L +L+
Sbjct: 328 S-EDNLNVRLNSLLVERDESKKELDAIQKEYSEFKSISEKKVASDAKLLGEQEKRLHQLE 386
Query: 263 ERTNLLICSDHLAASGMLGCSLGKD 287
E+ L SD + + +L L ++
Sbjct: 387 EQ--LGTASDEVRKNNVLIADLTQE 409
>IFH1_YEAST (P39520) IFH1 protein (RRP3 protein)
Length = 1085
Score = 33.1 bits (74), Expect = 4.0
Identities = 36/137 (26%), Positives = 59/137 (42%), Gaps = 9/137 (6%)
Query: 109 GKGLMNASVKKHGIGKGLMNASVKKHGIGKGLMTIWRATNPDSGELPVGFGFADREVHLI 168
GK S H G + A++K+ I KG +P P R LI
Sbjct: 3 GKKSPRKSTINHSTHSGKLPANIKRL-IKKGESDTKSRQSP-----PTLSTTRPRRFSLI 56
Query: 169 SNSKKSVRENNRSSKTATMN-RMLKNKLQNKRNNLQDKRKLLMQRKAGESNQHVTRN--Q 225
+S+ S+ + + S K + N +K K +N NN Q K+ L+QR+ ++ + Q
Sbjct: 57 YSSESSLSDVSDSDKNKSTNPHKIKRKAKNISNNSQGKKSKLIQRQIDNDDEGTESSDYQ 116
Query: 226 SLKEKCELSLDSEISEE 242
++ + E + E SEE
Sbjct: 117 AVTDGEESENEEEESEE 133
>EVPL_MOUSE (Q9D952) Envoplakin (p210) (210 kDa cornified envelope
precursor)
Length = 2035
Score = 33.1 bits (74), Expect = 4.0
Identities = 32/112 (28%), Positives = 56/112 (49%), Gaps = 9/112 (8%)
Query: 172 KKSVRENNRSSK---TATMNRMLKNKLQNKRNNLQDKRKLLMQRKAGESNQHVTRNQ--- 225
K S ++NR++ ++RM N Q +R+ L+ ++KL R+ GE NQ + Q
Sbjct: 25 KGSPNKHNRAATQELALLISRMQANADQVERDILETQKKLQQDRQNGEQNQALQHQQETG 84
Query: 226 -SLKEKCELSLDSEISEEGVDRISMLIDDE-ELELRELQERTNLLICSDHLA 275
+LKE L D + + R+ +E E ++++L ER CS++ A
Sbjct: 85 RNLKEAEVLLKDLFLDVDKARRLKHPQAEEIEKDIKQLHERVT-QECSEYRA 135
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.132 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 121,969,715
Number of Sequences: 164201
Number of extensions: 5261307
Number of successful extensions: 16166
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 38
Number of HSP's that attempted gapping in prelim test: 16112
Number of HSP's gapped (non-prelim): 83
length of query: 1041
length of database: 59,974,054
effective HSP length: 120
effective length of query: 921
effective length of database: 40,269,934
effective search space: 37088609214
effective search space used: 37088609214
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 71 (32.0 bits)
Lotus: description of TM0004a.8