BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0106.7.5
(63 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL07089.1| putative nonsense-mediated mRNA decay protein [Ar... 86 3e-16
gb|AAM91278.1| putative nonsense-mediated mRNA decay protein [Ar... 86 3e-16
gb|AAP55126.1| hypothetical protein [Oryza sativa (japonica cult... 66 2e-10
pir||S56692 hypothetical protein (clone AFN2) - wild oat (fragme... 54 8e-07
ref|XP_422813.1| PREDICTED: similar to Hypothetical protein MGC7... 42 0.006
ref|XP_315383.2| ENSANGP00000021124 [Anopheles gambiae str. PEST... 41 0.007
emb|CAG77957.1| unnamed protein product [Yarrowia lipolytica CLI... 40 0.012
emb|CAA15695.1| EG:152A3.1 [Drosophila melanogaster] gi|17137506... 40 0.012
emb|CAB42049.1| Nmd3 protein [Drosophila melanogaster] 40 0.012
gb|EAL32282.1| GA17465-PA [Drosophila pseudoobscura] 40 0.016
gb|EAL93974.1| nonsense-mediated mRNA decay protein 3 [Aspergill... 40 0.021
emb|CAG60293.1| unnamed protein product [Candida glabrata CBS138... 39 0.028
gb|EAA75946.1| hypothetical protein FG09760.1 [Gibberella zeae P... 39 0.047
gb|AAH90467.1| Zgc:113103 [Danio rerio] gi|61806709|ref|NP_00101... 39 0.047
gb|AAH81303.1| Hypothetical protein MGC76205 [Xenopus tropicalis... 38 0.061
emb|CAF97404.1| unnamed protein product [Tetraodon nigroviridis] 38 0.061
emb|CAF97402.1| unnamed protein product [Tetraodon nigroviridis] 38 0.061
gb|EAA52689.1| hypothetical protein MG05817.4 [Magnaporthe grise... 38 0.061
gb|AAS52980.1| AER299Cp [Ashbya gossypii ATCC 10895] gi|45190902... 38 0.080
gb|EAA64831.1| hypothetical protein AN1711.2 [Aspergillus nidula... 37 0.10
>gb|AAL07089.1| putative nonsense-mediated mRNA decay protein [Arabidopsis
thaliana]
Length = 516
Score = 85.9 bits (211), Expect = 3e-16
Identities = 42/65 (64%), Positives = 54/65 (82%), Gaps = 3/65 (4%)
Query: 1 QFLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELG---EDED 57
+FL DLEENP+LRFN+SLY++ ++Q SE AS+TDGE PSVP+EELLADL+L ED+D
Sbjct: 449 EFLRDLEENPELRFNISLYRDKDYQASETASMTDGEGAPSVPIEELLADLDLSFEEEDDD 508
Query: 58 EEDFM 62
+ED M
Sbjct: 509 DEDDM 513
>gb|AAM91278.1| putative nonsense-mediated mRNA decay protein [Arabidopsis
thaliana] gi|20466624|gb|AAM20629.1| putative
nonsense-mediated mRNA decay protein [Arabidopsis
thaliana] gi|4582430|gb|AAD24816.1| putative
nonsense-mediated mRNA decay protein [Arabidopsis
thaliana] gi|15227711|ref|NP_178476.1| nonsense-mediated
mRNA decay NMD3 family protein [Arabidopsis thaliana]
gi|25350038|pir||F84452 probable nonsense-mediated mRNA
decay protein [imported] - Arabidopsis thaliana
Length = 516
Score = 85.9 bits (211), Expect = 3e-16
Identities = 42/65 (64%), Positives = 54/65 (82%), Gaps = 3/65 (4%)
Query: 1 QFLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELG---EDED 57
+FL DLEENP+LRFN+SLY++ ++Q SE AS+TDGE PSVP+EELLADL+L ED+D
Sbjct: 449 EFLRDLEENPELRFNISLYRDKDYQASETASMTDGEGAPSVPIEELLADLDLSFEEEDDD 508
Query: 58 EEDFM 62
+ED M
Sbjct: 509 DEDDM 513
>gb|AAP55126.1| hypothetical protein [Oryza sativa (japonica cultivar-group)]
gi|37537074|ref|NP_922839.1| hypothetical protein [Oryza
sativa (japonica cultivar-group)]
gi|12643043|gb|AAK00432.1| hypothetical protein [Oryza
sativa]
Length = 711
Score = 66.2 bits (160), Expect = 2e-10
Identities = 31/60 (51%), Positives = 49/60 (81%), Gaps = 2/60 (3%)
Query: 1 QFLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELGEDEDEED 60
+FL DLE N D+RF + LY++ +++ SEMAS T G+++P+VP+EEL+ DL LG+DE+E++
Sbjct: 642 EFLRDLEHNSDIRFQIDLYKDEDYR-SEMAS-TIGDDVPTVPIEELIEDLSLGDDEEEDE 699
>pir||S56692 hypothetical protein (clone AFN2) - wild oat (fragment)
gi|726476|gb|AAA76741.1| putative ORF1
Length = 59
Score = 54.3 bits (129), Expect = 8e-07
Identities = 25/50 (50%), Positives = 43/50 (86%), Gaps = 2/50 (4%)
Query: 11 DLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELGEDEDEED 60
+LRF ++LY+N +++ SEMAS T G+++P+VP+EEL+ DL LG+D+++E+
Sbjct: 1 ELRFGINLYKNEDYR-SEMAS-TIGDDVPTVPIEELIEDLSLGDDDEDEE 48
>ref|XP_422813.1| PREDICTED: similar to Hypothetical protein MGC76205 [Gallus gallus]
Length = 668
Score = 41.6 bits (96), Expect = 0.006
Identities = 21/54 (38%), Positives = 33/54 (60%), Gaps = 3/54 (5%)
Query: 2 FLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELGED 55
FL DLEE+ +R N+++Y+N + + S TD + P + L E+L DL + ED
Sbjct: 606 FLEDLEEDEAIRKNVNIYRNADI---PVESDTDDDGAPRISLAEMLEDLHISED 656
>ref|XP_315383.2| ENSANGP00000021124 [Anopheles gambiae str. PEST]
gi|55238189|gb|EAA11292.3| ENSANGP00000021124 [Anopheles
gambiae str. PEST]
Length = 509
Score = 41.2 bits (95), Expect = 0.007
Identities = 24/61 (39%), Positives = 39/61 (63%), Gaps = 2/61 (3%)
Query: 1 QFLNDLEENPDLRFNLSLYQNIEFQPSEM-ASVTDGEELPSVPLEELLADLELGEDEDEE 59
+FL DLEE+P+LR N++++++ Q + + D +P + LEE+L DL L EDE+
Sbjct: 450 EFLEDLEEDPELRQNVNIFKDKNKQQIPVDTNDMDDPSVPRITLEEMLDDLVL-EDEEMG 508
Query: 60 D 60
D
Sbjct: 509 D 509
>emb|CAG77957.1| unnamed protein product [Yarrowia lipolytica CLIB99]
gi|50555484|ref|XP_505150.1| hypothetical protein
[Yarrowia lipolytica]
Length = 505
Score = 40.4 bits (93), Expect = 0.012
Identities = 26/67 (38%), Positives = 39/67 (57%), Gaps = 11/67 (16%)
Query: 1 QFLNDLEENPDLRFNLSLYQNIEFQPSEM--------ASVTDGEELPSVPLEELLADLE- 51
QFL +LEE+ +LR ++LY+ Q + AS +GE +P + ++ELL DLE
Sbjct: 439 QFLQELEEDTELRGTVNLYKRTVPQMPALPKTGDEMEASEEEGEGVPEIGVDELLDDLED 498
Query: 52 --LGEDE 56
LG+DE
Sbjct: 499 MSLGQDE 505
>emb|CAA15695.1| EG:152A3.1 [Drosophila melanogaster] gi|17137506|ref|NP_477332.1|
CG3460-PA [Drosophila melanogaster]
gi|7290283|gb|AAF45744.1| CG3460-PA [Drosophila
melanogaster] gi|16197917|gb|AAL13723.1| GM14705p
[Drosophila melanogaster]
Length = 521
Score = 40.4 bits (93), Expect = 0.012
Identities = 22/59 (37%), Positives = 39/59 (65%), Gaps = 3/59 (5%)
Query: 1 QFLNDLEENPDLRFNLSLYQNI-EFQPSEMASVTDGEELPSVPLEELLADLELGEDEDE 58
+FL+DLEEN D+R +++Y++ + P E+ + G ++P + LEE+L D+ L +DE
Sbjct: 451 EFLDDLEENEDIRGQVNIYRDTNKTIPIEVQAA--GGDVPQITLEEMLEDMTLECADDE 507
>emb|CAB42049.1| Nmd3 protein [Drosophila melanogaster]
Length = 521
Score = 40.4 bits (93), Expect = 0.012
Identities = 22/59 (37%), Positives = 39/59 (65%), Gaps = 3/59 (5%)
Query: 1 QFLNDLEENPDLRFNLSLYQNI-EFQPSEMASVTDGEELPSVPLEELLADLELGEDEDE 58
+FL+DLEEN D+R +++Y++ + P E+ + G ++P + LEE+L D+ L +DE
Sbjct: 451 EFLDDLEENEDIRGQVNIYRDTNKTIPIEVQAA--GGDVPQITLEEMLEDMTLECADDE 507
>gb|EAL32282.1| GA17465-PA [Drosophila pseudoobscura]
Length = 523
Score = 40.0 bits (92), Expect = 0.016
Identities = 22/59 (37%), Positives = 38/59 (64%), Gaps = 3/59 (5%)
Query: 1 QFLNDLEENPDLRFNLSLYQNI-EFQPSEMASVTDGEELPSVPLEELLADLELGEDEDE 58
+FL+DLEEN D+R +++Y++ + P E+ T ++P + LEE+L D+ L +DE
Sbjct: 453 EFLDDLEENVDMRGQVNIYRDTNKTLPIEVQGATG--DVPQITLEEMLEDMTLECADDE 509
>gb|EAL93974.1| nonsense-mediated mRNA decay protein 3 [Aspergillus fumigatus
Af293]
Length = 532
Score = 39.7 bits (91), Expect = 0.021
Identities = 24/70 (34%), Positives = 36/70 (51%), Gaps = 13/70 (18%)
Query: 2 FLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDG------------EELPSVPLEELLAD 49
FL D+EE+ +LR L LY+N +P+ V G ++ P + +EELL D
Sbjct: 463 FLRDVEEDQELRSTLQLYKNKNARPNNRMQVEGGAGGMEMDEDESDDDAPKISMEELLDD 522
Query: 50 L-ELGEDEDE 58
EL D++E
Sbjct: 523 FDELNMDDEE 532
>emb|CAG60293.1| unnamed protein product [Candida glabrata CBS138]
gi|50289849|ref|XP_447356.1| unnamed protein product
[Candida glabrata]
Length = 519
Score = 39.3 bits (90), Expect = 0.028
Identities = 23/60 (38%), Positives = 36/60 (59%), Gaps = 2/60 (3%)
Query: 2 FLNDLEENPDLRFNLSLYQNIEFQ-PSEMASVTDGEELPSVPLEELLADL-ELGEDEDEE 59
FL +LEE+ +LR ++LY+N P E + E+ P + ++ELL +L E+ DE EE
Sbjct: 454 FLQELEEDSELRQTINLYKNQNAAVPQEAHEEEEDEDAPQIDIDELLDELDEMTLDEPEE 513
>gb|EAA75946.1| hypothetical protein FG09760.1 [Gibberella zeae PH-1]
gi|46136489|ref|XP_389936.1| hypothetical protein
FG09760.1 [Gibberella zeae PH-1]
Length = 517
Score = 38.5 bits (88), Expect = 0.047
Identities = 21/53 (39%), Positives = 32/53 (59%), Gaps = 2/53 (3%)
Query: 1 QFLNDLEENPDLRFNLSLYQNIEFQPSEM--ASVTDGEELPSVPLEELLADLE 51
QFL D+EE+ +LR L+LY+ + EM A + +E P V ++ELL D +
Sbjct: 454 QFLRDVEEDEELRATLALYKAQKRAEEEMSVAETEEDDEAPHVDMDELLDDFD 506
>gb|AAH90467.1| Zgc:113103 [Danio rerio] gi|61806709|ref|NP_001013587.1|
hypothetical protein LOC541444 [Danio rerio]
Length = 505
Score = 38.5 bits (88), Expect = 0.047
Identities = 21/54 (38%), Positives = 33/54 (60%), Gaps = 1/54 (1%)
Query: 1 QFLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELGE 54
+FL DLEE+ LR N++++++ P E S TD + P + L E+L DL L +
Sbjct: 441 EFLEDLEEDEMLRKNINIFRDASKIPVE-ESDTDDDGAPRISLAEMLEDLSLSD 493
>gb|AAH81303.1| Hypothetical protein MGC76205 [Xenopus tropicalis]
gi|39849988|gb|AAH64238.1| Hypothetical protein MGC76205
[Xenopus tropicalis] gi|45361581|ref|NP_989367.1|
hypothetical protein LOC394998 [Xenopus tropicalis]
Length = 504
Score = 38.1 bits (87), Expect = 0.061
Identities = 21/54 (38%), Positives = 31/54 (56%), Gaps = 3/54 (5%)
Query: 2 FLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELGED 55
FL DLEE+ +R N+++Y+N + S TD E P + L E+L DL + D
Sbjct: 442 FLEDLEEDEVIRKNVNIYKNANL---PVESDTDEEGAPRISLAEMLEDLHISLD 492
>emb|CAF97404.1| unnamed protein product [Tetraodon nigroviridis]
Length = 549
Score = 38.1 bits (87), Expect = 0.061
Identities = 22/58 (37%), Positives = 34/58 (57%), Gaps = 2/58 (3%)
Query: 2 FLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELGEDEDEE 59
FL DLEE+ LR N++++++ P E S TD + P + L E+L +L L + EE
Sbjct: 487 FLEDLEEDEALRKNVNIFRDASKIPVE--SDTDDDGAPQISLAEMLEELSLTDATGEE 542
>emb|CAF97402.1| unnamed protein product [Tetraodon nigroviridis]
Length = 513
Score = 38.1 bits (87), Expect = 0.061
Identities = 22/58 (37%), Positives = 34/58 (57%), Gaps = 2/58 (3%)
Query: 2 FLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADLELGEDEDEE 59
FL DLEE+ LR N++++++ P E S TD + P + L E+L +L L + EE
Sbjct: 451 FLEDLEEDEALRKNVNIFRDASKIPVE--SDTDDDGAPQISLAEMLEELSLTDATGEE 506
>gb|EAA52689.1| hypothetical protein MG05817.4 [Magnaporthe grisea 70-15]
gi|39976519|ref|XP_369647.1| hypothetical protein
MG05817.4 [Magnaporthe grisea 70-15]
Length = 525
Score = 38.1 bits (87), Expect = 0.061
Identities = 23/69 (33%), Positives = 36/69 (51%), Gaps = 11/69 (15%)
Query: 1 QFLNDLEENPDLRFNLSLYQN-----------IEFQPSEMASVTDGEELPSVPLEELLAD 49
QFL D+EE+ +LR L+LY+N + +EM + + P V ++ELL D
Sbjct: 457 QFLRDVEEDEELRATLALYKNTKKTKKPDADAMSVAETEMTYDDEDDNAPKVSMDELLDD 516
Query: 50 LELGEDEDE 58
+E ED+
Sbjct: 517 MEELAIEDQ 525
>gb|AAS52980.1| AER299Cp [Ashbya gossypii ATCC 10895] gi|45190902|ref|NP_985156.1|
AER299Cp [Eremothecium gossypii]
Length = 512
Score = 37.7 bits (86), Expect = 0.080
Identities = 22/60 (36%), Positives = 38/60 (62%), Gaps = 2/60 (3%)
Query: 2 FLNDLEENPDLRFNLSLYQNIEFQPSEMASVTDGEELPSVPLEELLADL-ELGEDEDEED 60
FL +LEE+ ++R ++LY+N + SE TD +E P + ++ELL +L E+ D+ E+
Sbjct: 450 FLQELEEDQEMRQAINLYKNSKPVDSEEMQETD-DEAPQIDIDELLDELDEMNLDDPVEE 508
>gb|EAA64831.1| hypothetical protein AN1711.2 [Aspergillus nidulans FGSC A4]
gi|67522509|ref|XP_659315.1| hypothetical protein
AN1711_2 [Aspergillus nidulans FGSC A4]
gi|49087936|ref|XP_405848.1| hypothetical protein
AN1711.2 [Aspergillus nidulans FGSC A4]
Length = 511
Score = 37.4 bits (85), Expect = 0.10
Identities = 21/61 (34%), Positives = 36/61 (58%), Gaps = 5/61 (8%)
Query: 2 FLNDLEENPDLRFNLSLYQNIEFQPSEMASVT-----DGEELPSVPLEELLADLELGEDE 56
FL D+EE+ +LR L+LY+N + + + T D +E+P + ++ELL D + E +
Sbjct: 447 FLRDVEEDQELRATLALYKNKKKDDTMSVADTDMMNDDDDEVPKISMDELLDDFDDLEID 506
Query: 57 D 57
D
Sbjct: 507 D 507
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.310 0.135 0.364
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 117,534,158
Number of Sequences: 2540612
Number of extensions: 4209661
Number of successful extensions: 11506
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 11484
Number of HSP's gapped (non-prelim): 35
length of query: 63
length of database: 863,360,394
effective HSP length: 39
effective length of query: 24
effective length of database: 764,276,526
effective search space: 18342636624
effective search space used: 18342636624
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0106.7.5