BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0039.5
(582 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAP37714.1| At3g49220 [Arabidopsis thaliana] gi|6723392|emb|C... 788 0.0
gb|AAK93754.1| putative pectinesterase [Arabidopsis thaliana] gi... 770 0.0
ref|NP_913537.1| unnamed protein product [Oryza sativa (japonica... 558 e-157
ref|XP_465003.1| putative pectinesterase 2 precursor [Oryza sati... 531 e-149
emb|CAE76634.1| pectin methylesterase [Cicer arietinum] 472 e-131
emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum] 464 e-129
gb|AAF23892.1| pectin methyl esterase [Solanum tuberosum] 462 e-128
sp|P83948|PME3_CITSI Pectinesterase-3 precursor (Pectin methyles... 462 e-128
gb|AAB57670.1| pectinesterase [Citrus sinensis] 462 e-128
sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methyles... 461 e-128
ref|NP_915736.1| putative pectin esterase [Oryza sativa (japonic... 459 e-127
ref|XP_479497.1| putative pectinesterase [Oryza sativa (japonica... 459 e-127
dbj|BAD53265.1| putative pectin esterase [Oryza sativa (japonica... 459 e-127
gb|AAK84486.1| putative thermostable pectinesterase [Citrus sine... 458 e-127
gb|AAO85706.1| pectin methyl-esterase [Nicotiana benthamiana] 456 e-126
gb|AAL02367.1| pectin methylesterase [Lycopersicon esculentum] g... 454 e-126
gb|AAN28889.1| At3g14310/MLN21_9 [Arabidopsis thaliana] gi|92795... 453 e-126
gb|AAC72288.1| putative pectin methylesterase [Arabidopsis thali... 449 e-125
emb|CAE76633.2| pectin methylesterase [Cicer arietinum] 448 e-124
gb|AAK81875.1| pectin methylesterase PME1 [Vitis vinifera] 447 e-124
>gb|AAP37714.1| At3g49220 [Arabidopsis thaliana] gi|6723392|emb|CAB66401.1|
pectinesterase-like protein [Arabidopsis thaliana]
gi|16648929|gb|AAL24316.1| pectinesterase-like protein
[Arabidopsis thaliana] gi|15229105|ref|NP_190491.1|
pectinesterase family protein [Arabidopsis thaliana]
gi|11279196|pir||T45827 pectinesterase-like protein -
Arabidopsis thaliana
Length = 598
Score = 788 bits (2034), Expect = 0.0
Identities = 393/598 (65%), Positives = 475/598 (78%), Gaps = 18/598 (3%)
Query: 1 MEYGKLGPSDSGGGSSRSDVALVNSRTKTTSGR--------KKKLIILVLLAVVLIVASA 52
M Y +LGPS + G + S +TS + KKKL++ ++ + ++ +A
Sbjct: 1 MGYERLGPSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAA 60
Query: 53 VVLTAVRSRAG-GSGASTLRAKPTQAISNTCSKTRFPSLCVKSLLDFPGSTAASE-RDLV 110
+ VRSR L KP+QAIS C TRFP LCV SL+DFPGS AAS +DL+
Sbjct: 61 AIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLI 120
Query: 111 HISLNMTFQHLAKALYSSAAIS-ASMDPRVRAAYDDCLELLDDSVDALGRSLSSFAAGSS 169
H+++NMT H + ALYSSA++S M PR R+AYD C+ELLDDSVDAL R+LSS + S+
Sbjct: 121 HVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSA 180
Query: 170 S-SDVMTWLSAALTNQDTCADGFADTS-GDVKDQMAGNLKDLSELVSNCLAIFAGA--GD 225
DV TWLSAALTN DTC +GF G VKD M L++LSELVSNCLAIF+ + GD
Sbjct: 181 KPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGD 240
Query: 226 DFSGVPIQNRRLLAM--RDDNFPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAE 283
DF+GVPIQNRRLL + R++ FPRW+ ++R +L +PVS +QAD++VSKDGNGT KTI+E
Sbjct: 241 DFAGVPIQNRRLLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISE 300
Query: 284 AIKKVPEYGSRRFIIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLT 343
AIKK P+ +RR IIYV+AGRYEENNLK+GRK+ N+M +GDGKGKTVI+GGK++ D + T
Sbjct: 301 AIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI-T 359
Query: 344 TFHTASFAASGAGFIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHS 403
TFHTASFAA+GAGFIARD+TFEN+AGP KHQAVALR+GADHAV+YRCN+IGYQDT+YVHS
Sbjct: 360 TFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHS 419
Query: 404 NRQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIH 463
NRQF+RECDIYGTVDFIFGNAAVV QNCS+YARKPM QKNTITAQNRKDPNQNTGISIH
Sbjct: 420 NRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIH 479
Query: 464 ACRVLAAADLKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLY 523
A RVLAA+DL+A+N S +TYLGRPWKL+SRTVYMMSYIG HVH RGWLEWNTTFALDTLY
Sbjct: 480 ASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLY 539
Query: 524 YGEYMNYGPGAAVGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 581
YGEY+N GPG+ +GQRV+WPGYRVI S EA+RFTVA+FI G++WLPSTGV+FL+GLS
Sbjct: 540 YGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597
>gb|AAK93754.1| putative pectinesterase [Arabidopsis thaliana]
gi|13507549|gb|AAK28637.1| putative pectinesterase
[Arabidopsis thaliana] gi|9759184|dbj|BAB09799.1|
pectinesterase [Arabidopsis thaliana]
gi|15238729|ref|NP_200149.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 587
Score = 770 bits (1988), Expect = 0.0
Identities = 387/590 (65%), Positives = 456/590 (76%), Gaps = 13/590 (2%)
Query: 1 MEYGKLGPSDSGGGSSRSDVALVNSRTKTTSGRKKKLIILVLLAVVLIVASAVVLTAVRS 60
M Y +LGPS + + + K T K KLI+ L +V+ V + +R+
Sbjct: 1 MGYDRLGPSGPSNPNQKDPATSLPELQKKT---KTKLILFTLAVLVVGVVCFGIFAGIRA 57
Query: 61 RAGGSGASTLRAKPTQAISNTCSKTRFPSLCVKSLLDFPGSTAASERDLVHISLNMTFQH 120
G L KPTQAIS TCSK+ +P+LC+ +LLDFPGS A E +L+HIS N T Q
Sbjct: 58 VDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQK 117
Query: 121 LAKALYSSAAIS-ASMDPRVRAAYDDCLELLDDSVDALGRSLSSFAAGS---SSSDVMTW 176
+KALY+S+ I+ M PRVR+AYD CLELLDDSVDAL R+LSS S S SDVMTW
Sbjct: 118 FSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTW 177
Query: 177 LSAALTNQDTCADGFADTSG---DVKDQMAGNLKDLSELVSNCLAIFAGAGDDFSGVPI- 232
LS+A+TN DTC DGF + G +VKDQ+ G +KDLSE+VSNCLAIFAG D SGVP+
Sbjct: 178 LSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVV 237
Query: 233 QNRRLLAMRD-DNFPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEY 291
NR+LL + + P WL R DR LL P SA+QAD+ VSKDG+GT KTIAEAIKK PE+
Sbjct: 238 NNRKLLGTEETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEH 297
Query: 292 GSRRFIIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFA 351
SRRF+IYV+AGRYEE NLK+GRK+TN+M IGDGKGKTVITGGK++ D L TTFHTA+FA
Sbjct: 298 SSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDL-TTFHTATFA 356
Query: 352 ASGAGFIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYREC 411
A+GAGFI RDMTFENYAGP KHQAVALRVG DHAVVYRCN+IGYQD +YVHSNRQF+REC
Sbjct: 357 ATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFREC 416
Query: 412 DIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAA 471
+IYGTVDFIFGNAAV+ Q+C++YARKPMAQQK TITAQNRKDPNQNTGISIHAC++LA
Sbjct: 417 EIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATP 476
Query: 472 DLKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYG 531
DL+AS S+ TYLGRPWKLYSR VYMMS +GDH+ RGWLEWN FALD+LYYGEYMN G
Sbjct: 477 DLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKG 536
Query: 532 PGAAVGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 581
G+ +GQRV WPGY VITS +EAS+FTVAQFI G++WLPSTGV+F SGLS
Sbjct: 537 LGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586
>ref|NP_913537.1| unnamed protein product [Oryza sativa (japonica cultivar-group)]
Length = 621
Score = 558 bits (1439), Expect = e-157
Identities = 307/608 (50%), Positives = 397/608 (64%), Gaps = 55/608 (9%)
Query: 26 RTKTTSGRKKKLIILVL------LAVVLIVASAVVL---TAVRSRAGGSGASTLRAKPTQ 76
R + GR ++ +I+VL LAV + VA A+ + + + S +GG A RA PT+
Sbjct: 16 RLRCADGRHRRRLIVVLCIVGVALAVGVAVAVAIAVLGRSRMTSSSGGGRAPRGRA-PTE 74
Query: 77 AISNTCSKTRFPSLCVKSLLDFPGSTAASERDLVHISLNMTFQHLAKALYSSAAISASM- 135
AI+ TC T +P LCV L+ FPG+ A + +LV +SLN T + + ALY++ A+ +
Sbjct: 75 AIARTCGVTLYPELCVGELMAFPGAAGAGDAELVPMSLNATHRRVVDALYNATALGGAAA 134
Query: 136 ---DPRVRAAYDDCLELLDDSVDALGRSLSSFAA-------------GSSSSDVMTWLSA 179
R AAY DC+E+LD + + L RS+ + AA G D+MTWLSA
Sbjct: 135 LLAGARSGAAYGDCVEMLDAAEELLARSVGAIAAPPPPPDSVDADTAGRDDDDIMTWLSA 194
Query: 180 ALTNQDTCADGFA----------DTSGDVKDQMAGNLKDLSELVSNCLAIFAGAG---DD 226
ALT+ DTC D D G +K QM G L +L E +SN LAIFA G +
Sbjct: 195 ALTSHDTCMDSLQEVGAGGDAGDDDGGRIKPQMLGYLGNLGEHLSNSLAIFAARGRPGGE 254
Query: 227 FSGVPIQN---RRLLAMRDDN-----FPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTA 278
S VP+ N RRLL + DD+ FPRW+ DRRLL + ++AD+VV+KDG GT
Sbjct: 255 LSDVPVHNQLHRRLLTIDDDDDDDGSFPRWVRHNDRRLLQAAAAEIEADMVVAKDGTGTH 314
Query: 279 KTIAEAIKKVPEYGSRRFIIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVM 338
+ I +AIK PE+ RR +IYV+AG Y EN +KIG K+TN+M++GDG GKTV+ G ++V
Sbjct: 315 RKIRDAIKAAPEHSRRRVVIYVKAGVYTEN-VKIGSKKTNLMLVGDGAGKTVVVGYRSVH 373
Query: 339 DGLLTTFHTASFAASGAGFIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDT 398
D TTFHTA+ A +GAGFI RDMT EN AG +HQAVAL + DHAVVYR V+GYQDT
Sbjct: 374 DNY-TTFHTATLAVAGAGFIMRDMTVENRAGAARHQAVALLLSGDHAVVYRSAVLGYQDT 432
Query: 399 MYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNT 458
+Y H+ RQFYR+CD+ GTVDF+FGNAAVV QNC+L+AR+P+ Q+NT+TAQ R+DPNQ+T
Sbjct: 433 LYAHAQRQFYRDCDVAGTVDFVFGNAAVVLQNCTLWARRPLPGQENTVTAQGRRDPNQST 492
Query: 459 GISIHACRVLAAADLK----ASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWN 514
GIS+H CR+L + +L+ A TYLGRPWK YSR VYMMSYI HVH GWL W+
Sbjct: 493 GISVHGCRLLPSPELELAPAARRGRAATYLGRPWKPYSRAVYMMSYIAGHVHAAGWLAWD 552
Query: 515 TT-FALDTLYYGEYMNYGPGAAVGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTG 573
+ A DTLYYGEY N GPGAAVG RV WPG+RVI EA FTV +FI G +WLP TG
Sbjct: 553 ASGRAPDTLYYGEYRNSGPGAAVGGRVPWPGHRVIKLPEEAMEFTVGRFIGGYSWLPPTG 612
Query: 574 VAFLSGLS 581
VAF++GL+
Sbjct: 613 VAFVAGLT 620
>ref|XP_465003.1| putative pectinesterase 2 precursor [Oryza sativa (japonica
cultivar-group)] gi|47847929|dbj|BAD21719.1| putative
pectinesterase 2 precursor [Oryza sativa (japonica
cultivar-group)]
Length = 554
Score = 531 bits (1369), Expect = e-149
Identities = 282/530 (53%), Positives = 356/530 (66%), Gaps = 51/530 (9%)
Query: 98 FPGST-----AASERDLVHISLNMTFQHLAKAL--YSSAAI--------------SASMD 136
FP S A+ + I+LN T H+ KAL S AA+ S+S
Sbjct: 29 FPASALEAEAVAARHHRLRIALNATAVHVGKALEALSGAAVAPASAVMTTPFSAGSSSSS 88
Query: 137 PRVRAAYDDCLELLDDSVDALGRSLSSFAAGSSSSDVMTWLSAALTNQDTCADGFADTSG 196
P AA DDC ELL+DS+D L + AA D +TWLSAALTN DTCAD +
Sbjct: 89 PLAAAARDDCAELLEDSLDLLAGAGEPSAA---HDDAVTWLSAALTNHDTCADSLNEAGI 145
Query: 197 DVKDQMAGNLKDLSELVSNCLAIFAGAG---------DDFSGVPIQN---------RRLL 238
A +L +V +CL ++A A D +GVP++N +R
Sbjct: 146 PHGADAAPHLAAARAMVRDCLTMYAEAASAAMATSNKDGLAGVPVRNGGGKSKKQRKRGR 205
Query: 239 AMRDDNFPRWLNRRDRRLLSLPVSAM--QADVVVSKDGNGTAKTIAEAIKKVPEYGSRRF 296
R FPRWL+ RDRRLL +P + + AD+VV+KDG GT +TI++A+K PE RR
Sbjct: 206 RRRRSLFPRWLSARDRRLLLVPAAPLVESADMVVAKDGTGTHRTISDAVKAAPERSGRRT 265
Query: 297 IIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAG 356
+I+V+AGRY+EN +K+GRK+TN++ +GDGKG TV++ G++V D TTFHTA+FAASG+G
Sbjct: 266 VIHVKAGRYDEN-VKVGRKKTNLVFVGDGKGVTVVSAGRSVADNF-TTFHTATFAASGSG 323
Query: 357 FIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGT 416
F+ RDMT EN+AGPE+HQAVALRV AD A VYRC++IGYQDT+Y HSNR FYR+CD+YGT
Sbjct: 324 FMMRDMTVENWAGPERHQAVALRVSADRAAVYRCSIIGYQDTLYAHSNRHFYRDCDVYGT 383
Query: 417 VDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKAS 476
VDF+FGNAA V Q C+L++R P+ QKNT+TAQNR+DP Q+TG+ IHACRV+ + ++
Sbjct: 384 VDFVFGNAAAVLQRCNLWSRSPLPGQKNTVTAQNRRDPGQSTGLVIHACRVVPSPPPPST 443
Query: 477 NESF-----ETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYG 531
+ TYLGRPWKLYSR V MMSYIG HV GWL WN TFALDTLYYGEYMNYG
Sbjct: 444 APAVAAPLAPTYLGRPWKLYSRVVVMMSYIGGHVPPEGWLAWNATFALDTLYYGEYMNYG 503
Query: 532 PGAAVGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 581
PGA V RV WPG+RVI + EA RFTVA+FI GA+WLP+TGV+FLSGLS
Sbjct: 504 PGAGVAGRVAWPGHRVINDSAEAERFTVARFISGASWLPATGVSFLSGLS 553
>emb|CAE76634.1| pectin methylesterase [Cicer arietinum]
Length = 254
Score = 472 bits (1214), Expect = e-131
Identities = 222/255 (87%), Positives = 239/255 (93%), Gaps = 1/255 (0%)
Query: 328 KTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTFENYAGPEKHQAVALRVGADHAVV 387
KTVITGGKNVM L TTFHTASFAASG GFIARD+TFENYAGP KHQAVALRVGADHAVV
Sbjct: 1 KTVITGGKNVMQNL-TTFHTASFAASGPGFIARDVTFENYAGPAKHQAVALRVGADHAVV 59
Query: 388 YRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTIT 447
YRCN+IGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTIT
Sbjct: 60 YRCNIIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTIT 119
Query: 448 AQNRKDPNQNTGISIHACRVLAAADLKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQ 507
AQNRKDPNQNTGISIH CR+LA DL+AS SF+TYLGRPWKLYS+TVYM+SY+GDH+H
Sbjct: 120 AQNRKDPNQNTGISIHNCRILATPDLEASKGSFQTYLGRPWKLYSKTVYMLSYMGDHIHP 179
Query: 508 RGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPGYRVITSALEASRFTVAQFILGAT 567
RGWLEWN TFALDTLYYGEYMNYGPG A+GQRV WPG+RVITS +EA+RFTVAQFI G++
Sbjct: 180 RGWLEWNATFALDTLYYGEYMNYGPGGAIGQRVTWPGFRVITSTVEANRFTVAQFISGSS 239
Query: 568 WLPSTGVAFLSGLST 582
WLPSTGVAF++GLST
Sbjct: 240 WLPSTGVAFVAGLST 254
>emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum]
Length = 579
Score = 464 bits (1193), Expect = e-129
Identities = 252/575 (43%), Positives = 355/575 (60%), Gaps = 37/575 (6%)
Query: 34 KKKLIILVLLAVVLIVASAVVLTAVRSRAGGSG----ASTLRAKPTQAISNTCSKTRFPS 89
KKKL + V+ +V+L+ A V+ V+ R+ S + + + + C T P
Sbjct: 14 KKKLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAITSAAHAIVKSACENTLHPE 73
Query: 90 LC---VKSLLDFPGSTAASERDLVHISLNMT---FQHLAKALYSSAAISASMDPRVRAAY 143
LC + S+ DF S++D++ +SLN+T QH + + PR + A
Sbjct: 74 LCYSTIASVSDF-SKKVTSQKDVIELSLNITCRAVQHNFFKVEKLIKTRKGLKPREKVAL 132
Query: 144 DDCLELLDDSVDALGRSLSSF-------AAGSSSSDVMTWLSAALTNQDTCADGFADTSG 196
DCLE +D+++D L ++ + + + D+ T +S+A+TNQ+TC DGF+
Sbjct: 133 HDCLETIDETLDELHTAIKDLELYPNKKSLKAHADDLKTLISSAITNQETCLDGFSHDDA 192
Query: 197 D--VKDQMAGNLKDLSELVSNCLAIFAGAGDDFSGVPIQNRRLLA-------MRDDN--F 245
D V+ + K + ++ SN LA+ D I N + L +R+DN +
Sbjct: 193 DKKVRKALLKGQKHVEKMCSNALAMICNMTD----TDIANEQKLKGTTTNRKLREDNSEW 248
Query: 246 PRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGRY 305
P WL+ DRRLL S ++ DVVV+ DG+G KT++EA+ K PE S+R++I ++AG Y
Sbjct: 249 PEWLSAGDRRLLQS--STVRPDVVVAADGSGNFKTVSEAVAKAPEKSSKRYVIRIKAGVY 306
Query: 306 EENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTFE 365
EN + + +K+TN+M +GDG+ T+ITG +NV DG TTFH+A+ AA G F+ARD+TF+
Sbjct: 307 REN-VDVPKKKTNIMFMGDGRSNTIITGSRNVKDGS-TTFHSATVAAVGEKFLARDITFQ 364
Query: 366 NYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAA 425
N AG KHQAVALRVG+D + YRC+++ YQD++YVHSNRQ++ +C I GTVDFIFGNAA
Sbjct: 365 NTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIAGTVDFIFGNAA 424
Query: 426 VVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETYLG 485
V QNC ++AR+P + QKN +TAQ R DPNQNTGI I CR+ A +DL+ +SF TYLG
Sbjct: 425 AVLQNCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLG 484
Query: 486 RPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPGY 545
RPWK YSRTV M S I D ++ GW EWN FAL+TL+YGEY N G GA RV W G+
Sbjct: 485 RPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGAGTSGRVKWKGF 544
Query: 546 RVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
+VITSA EA +T +FI G +WL STG F GL
Sbjct: 545 KVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 579
>gb|AAF23892.1| pectin methyl esterase [Solanum tuberosum]
Length = 576
Score = 462 bits (1190), Expect = e-128
Identities = 243/570 (42%), Positives = 355/570 (61%), Gaps = 24/570 (4%)
Query: 31 SGRKKKLIILVLLAVVLIVASAVVLTAVRSRAGGSGAST----LRAKPTQAISNTCSKTR 86
S RKKK+ + ++ +V+L+ A V+ V+SR+ S + + + + CS T
Sbjct: 11 SKRKKKIYLAIVASVLLVAAVIGVVAGVKSRSNNSDDDADIMAISSSAHAIVKSACSNTL 70
Query: 87 FPSLCVKSLLDFP--GSTAASERDLVHISLNMTFQHLAKALYSSAAISAS---MDPRVRA 141
P LC ++++ S++D++ +SLN+T + + + Y+ + + + PR +
Sbjct: 71 HPELCYSAIVNVTDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKV 130
Query: 142 AYDDCLELLDDSVDALGRSLSSF-------AAGSSSSDVMTWLSAALTNQDTCADGFADT 194
A DCLE +D+++D L +++ + + D+ T +S+A+TNQ+TC DGF+
Sbjct: 131 ALHDCLETMDETLDELHTAVADLELYPNKKSLKEHAEDLKTLISSAITNQETCLDGFSHD 190
Query: 195 SGD--VKDQMAGNLKDLSELVSNCLAIFAGAG--DDFSGVPIQNRRLLAMRDDNFPRWLN 250
D V+ + K + ++ SN LA+ D + + + R L + +P WL+
Sbjct: 191 EADKKVRKVLLKGQKHVEKMCSNALAMICNMTNTDIANEMKLSGSRKLVEDNGEWPEWLS 250
Query: 251 RRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGRYEENNL 310
DRRLL S + DVVV+ DG+G KT++EA+ K PE S+R++I ++AG Y EN +
Sbjct: 251 AGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVAKAPEKSSKRYVIRIKAGVYREN-V 307
Query: 311 KIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTFENYAGP 370
+ +K+TN+M +GDG+ T+IT +NV DG TTFH+A+ AA G F+ARD+TF+N AG
Sbjct: 308 DVPKKKTNIMFMGDGRSNTIITASRNVQDGS-TTFHSATVAAVGEKFLARDITFQNTAGA 366
Query: 371 EKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQN 430
KHQAVALRVG+D + Y+C+++ YQDT+YVHSNRQF+ +C + GTVDFIFGN A V Q+
Sbjct: 367 SKHQAVALRVGSDLSAFYKCDILAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVLQD 426
Query: 431 CSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETYLGRPWKL 490
C ++AR+P + QKN +TAQ R DPNQNTGI I CR+ A +DL+ +SF TYLGRPWK
Sbjct: 427 CDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKE 486
Query: 491 YSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPGYRVITS 550
YSRTV M S I D + GW EWN FAL+TL+YGEY N G GAA RV W G++VITS
Sbjct: 487 YSRTVIMQSSITDVIQPAGWHEWNGNFALNTLFYGEYANTGAGAATSGRVKWKGHKVITS 546
Query: 551 ALEASRFTVAQFILGATWLPSTGVAFLSGL 580
+ EA +T FI G +WL STG F GL
Sbjct: 547 STEAQAYTPGSFIAGGSWLSSTGFPFSLGL 576
>sp|P83948|PME3_CITSI Pectinesterase-3 precursor (Pectin methylesterase 3) (PE 3)
Length = 584
Score = 462 bits (1189), Expect = e-128
Identities = 252/568 (44%), Positives = 354/568 (61%), Gaps = 28/568 (4%)
Query: 33 RKKKLIILVLLAVVLIVASAVVLTA---VRSRAGGSGASTLRAKPTQAISNTCSKTRFPS 89
+KKK + L L A +L+VA+ + + A R +G +G A + ++CS TR+P
Sbjct: 25 KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHA----ILKSSCSSTRYPD 80
Query: 90 LCVKSLLDFPGST--AASERDLVHISLNMTFQHLAKALYSSAAI--SASMDPRVRAAYDD 145
LC ++ P ++ S++D++ +SLN+T + + + ++ R + A D
Sbjct: 81 LCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHD 140
Query: 146 CLELLDDSVDALGRSLSSF-------AAGSSSSDVMTWLSAALTNQDTCADGFA--DTSG 196
CLE +D+++D L +++ + + D+ T +SAA+TNQ TC DGF+ D +
Sbjct: 141 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 200
Query: 197 DVKDQMAGNLKDLSELVSNCLAIFAGAGD-DFSGVPIQNRRLLAMRD---DNFPRWLNRR 252
V+D ++ + ++ SN LA+ D D + N R L D +P WL+
Sbjct: 201 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETSTVDGWPAWLSTG 260
Query: 253 DRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGRYEENNLKI 312
DRRLL S++ +VVV+ DG+G KT+A ++ P+ G++R+II ++AG Y EN +++
Sbjct: 261 DRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYREN-VEV 317
Query: 313 GRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTFENYAGPEK 372
+K N+M IGDG+ +T+ITG +NV+DG TTF +A+ A G GF+ARD+TF+N AGP K
Sbjct: 318 TKKHKNIMFIGDGRTRTIITGSRNVVDGS-TTFKSATVAVVGEGFLARDITFQNTAGPSK 376
Query: 373 HQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCS 432
HQAVALRVGAD + Y C+++ YQDT+YVHSNRQF+ C I GTVDFIFGNAA V QNC
Sbjct: 377 HQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCD 436
Query: 433 LYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETYLGRPWKLYS 492
++ARKP + QKN +TAQ R DPNQNTGI I R+ A +DLK SF TYLGRPWK YS
Sbjct: 437 IHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYS 496
Query: 493 RTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPGYRVITSAL 552
RTV M S I D +H GW EW+ FAL+TL+YGE+ N G GA RV W G+RVITSA
Sbjct: 497 RTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSAT 556
Query: 553 EASRFTVAQFILGATWLPSTGVAFLSGL 580
EA FT FI G++WL STG F GL
Sbjct: 557 EAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>gb|AAB57670.1| pectinesterase [Citrus sinensis]
Length = 584
Score = 462 bits (1188), Expect = e-128
Identities = 252/568 (44%), Positives = 353/568 (61%), Gaps = 28/568 (4%)
Query: 33 RKKKLIILVLLAVVLIVASAVVLTA---VRSRAGGSGASTLRAKPTQAISNTCSKTRFPS 89
+KKK + L L A +L+VA+ + + A R +G +G A + ++CS TR+P
Sbjct: 25 KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHA----ILKSSCSSTRYPD 80
Query: 90 LCVKSLLDFPGST--AASERDLVHISLNMTFQHLAKALYSSAAI--SASMDPRVRAAYDD 145
LC ++ P ++ S++D++ +SLN+T + + + ++ R + A D
Sbjct: 81 LCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHD 140
Query: 146 CLELLDDSVDALGRSLSSF-------AAGSSSSDVMTWLSAALTNQDTCADGFA--DTSG 196
CLE +D+++D L +++ + + D+ T +SAA+TNQ TC DGF+ D +
Sbjct: 141 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 200
Query: 197 DVKDQMAGNLKDLSELVSNCLAIFAGAGD-DFSGVPIQNRRLLAMRD---DNFPRWLNRR 252
V+D ++ + ++ SN LA+ D D + N R L D +P WL+
Sbjct: 201 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEETSTVDGWPAWLSPG 260
Query: 253 DRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGRYEENNLKI 312
DRRLL S++ + VV+ DG+G KT+A A+ P+ G++R+II ++AG Y EN +++
Sbjct: 261 DRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVYREN-VEV 317
Query: 313 GRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTFENYAGPEK 372
+K N+M IGDG+ +T+ITG +NV+DG TTF +A+ A G GF+ARD+TF+N AGP K
Sbjct: 318 TKKHKNIMFIGDGRTRTIITGSRNVVDGS-TTFKSATVAVVGEGFLARDITFQNTAGPSK 376
Query: 373 HQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCS 432
HQAVALRVGAD + Y C+++ YQDT+YVHSNRQF+ C I GTVDFIFGNAA V QNC
Sbjct: 377 HQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCD 436
Query: 433 LYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETYLGRPWKLYS 492
++ARKP + QKN +TAQ R DPNQNTGI I R+ A +DLK SF TYLGRPWK YS
Sbjct: 437 IHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYS 496
Query: 493 RTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPGYRVITSAL 552
RTV M S I D +H GW EW+ FAL+TL+YGE+ N G GA RV W G+RVITSA
Sbjct: 497 RTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSAT 556
Query: 553 EASRFTVAQFILGATWLPSTGVAFLSGL 580
EA FT FI G++WL STG F GL
Sbjct: 557 EAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methylesterase) (PE)
gi|2098705|gb|AAB57667.1| pectinesterase [Citrus
sinensis]
Length = 584
Score = 461 bits (1187), Expect = e-128
Identities = 252/568 (44%), Positives = 353/568 (61%), Gaps = 28/568 (4%)
Query: 33 RKKKLIILVLLAVVLIVASAVVLTA---VRSRAGGSGASTLRAKPTQAISNTCSKTRFPS 89
+KKK + L L A +L+VA+ + + A R +G +G A + ++CS TR+P
Sbjct: 25 KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHA----ILKSSCSSTRYPD 80
Query: 90 LCVKSLLDFPGST--AASERDLVHISLNMTFQHLAKALYSSAAI--SASMDPRVRAAYDD 145
LC ++ P ++ S++D++ +SLN+T + + + ++ R + A D
Sbjct: 81 LCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHD 140
Query: 146 CLELLDDSVDALGRSLSSF-------AAGSSSSDVMTWLSAALTNQDTCADGFA--DTSG 196
CLE +D+++D L +++ + + D+ T +SAA+TNQ TC DGF+ D +
Sbjct: 141 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 200
Query: 197 DVKDQMAGNLKDLSELVSNCLAIFAGAGD-DFSGVPIQNRRLLAMRD---DNFPRWLNRR 252
V+D ++ + ++ SN LA+ D D + N R L D +P WL+
Sbjct: 201 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEETSTVDGWPAWLSPG 260
Query: 253 DRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGRYEENNLKI 312
DRRLL S++ + VV+ DG+G KT+A A+ P+ G++R+II ++AG Y EN +++
Sbjct: 261 DRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVYREN-VEV 317
Query: 313 GRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTFENYAGPEK 372
+K N+M IGDG+ +T+ITG +NV+DG TTF +A+ A G GF+ARD+TF+N AGP K
Sbjct: 318 TKKHKNIMFIGDGRTRTIITGSRNVVDGS-TTFKSATAAVVGEGFLARDITFQNTAGPSK 376
Query: 373 HQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCS 432
HQAVALRVGAD + Y C+++ YQDT+YVHSNRQF+ C I GTVDFIFGNAA V QNC
Sbjct: 377 HQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCD 436
Query: 433 LYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETYLGRPWKLYS 492
++ARKP + QKN +TAQ R DPNQNTGI I R+ A +DLK SF TYLGRPWK YS
Sbjct: 437 IHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYS 496
Query: 493 RTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPGYRVITSAL 552
RTV M S I D +H GW EW+ FAL+TL+YGE+ N G GA RV W G+RVITSA
Sbjct: 497 RTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSAT 556
Query: 553 EASRFTVAQFILGATWLPSTGVAFLSGL 580
EA FT FI G++WL STG F GL
Sbjct: 557 EAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>ref|NP_915736.1| putative pectin esterase [Oryza sativa (japonica cultivar-group)]
Length = 563
Score = 459 bits (1180), Expect = e-127
Identities = 253/525 (48%), Positives = 324/525 (61%), Gaps = 25/525 (4%)
Query: 65 SGASTLRAKPTQAISNTCSKTRFPSLCVKSLLD--------FPGSTAASERDLVHISLNM 116
+GA+ L PT +N C+ + P+ C + D P A R ++ SL+
Sbjct: 55 AGAAFLLLSPTA--TNLCASSPDPASCQAIVADAVLASPHSHPSRPAHVLRAILATSLD- 111
Query: 117 TFQHLAKALYSSAAISASMDPRVRAAYDDCLELLDDSVDALGRSLSSFAAGSSSSDVMTW 176
+H A A + + DPR RAA +DC++L+ + D L + + D TW
Sbjct: 112 --RHDAAAEAVAGMRRRASDPRHRAALEDCVQLMGLARDRLADAAGAPDVDVDVDDARTW 169
Query: 177 LSAALTNQDTCADGFADTSGDVKDQMAGNLKDLSELVSNCLAIFAGAGDDFSGVPIQNRR 236
LSA LT+ TC DG D G ++D + +L+ L L S LA+ + AG R
Sbjct: 170 LSAVLTDHVTCLDGLDD--GPLRDSVGAHLEPLKSLASASLAVLSAAGRGA-------RD 220
Query: 237 LLAMRDDNFPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRF 296
+LA D FP WL RDR LL A+QADVVV+KDG+G TI EA+ P+ G R+
Sbjct: 221 VLAEAVDRFPSWLTARDRTLLDAGAGAVQADVVVAKDGSGKYTTIKEAVDAAPDGGKSRY 280
Query: 297 IIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAG 356
+IYV+ G Y+EN L++G+ + +MI+GDG +TVITG +NV+DG TTF++A+ A SG G
Sbjct: 281 VIYVKKGVYKEN-LEVGKTKRVLMIVGDGMDQTVITGSRNVVDGS-TTFNSATLALSGDG 338
Query: 357 FIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGT 416
I +D+ EN AG EK QAVALRV AD AV+ RC + GYQDT+Y H RQFYR+C + GT
Sbjct: 339 IILQDLKVENTAGAEKQQAVALRVSADRAVINRCRLDGYQDTLYAHQLRQFYRDCAVSGT 398
Query: 417 VDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKAS 476
VDF+FGNAA V Q C L AR+P QKN +TAQ R DPNQNTG SIH CRV+ A DL +
Sbjct: 399 VDFVFGNAAAVLQGCVLTARRPAQAQKNAVTAQGRTDPNQNTGTSIHRCRVVPAPDLAPA 458
Query: 477 NESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWN-TTFALDTLYYGEYMNYGPGAA 535
+ F T+LGRPWK YSRTVYM+SY+ HV RGWLEWN FAL TL+YGEY N GPGA+
Sbjct: 459 AKQFPTFLGRPWKEYSRTVYMLSYLDSHVDPRGWLEWNGADFALKTLFYGEYQNQGPGAS 518
Query: 536 VGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
RVNWPGY VIT A +FTV QFI G WL +TGV + GL
Sbjct: 519 TAGRVNWPGYHVITDQSVAMQFTVGQFIQGGNWLKATGVNYNEGL 563
>ref|XP_479497.1| putative pectinesterase [Oryza sativa (japonica cultivar-group)]
gi|50509816|dbj|BAD31979.1| putative pectinesterase
[Oryza sativa (japonica cultivar-group)]
gi|34393863|dbj|BAC83543.1| putative pectinesterase
[Oryza sativa (japonica cultivar-group)]
Length = 579
Score = 459 bits (1180), Expect = e-127
Identities = 261/578 (45%), Positives = 350/578 (60%), Gaps = 49/578 (8%)
Query: 37 LIILVLLAVVLIVASAVVLTAV------RSR---AGGSGASTLRAKPTQAISNTCSKTRF 87
L +L+LL ++ ++++A AV R+R A G A+ A+ C+ T
Sbjct: 18 LSLLLLLLLLSLLSTAHKAFAVADDDGHRARVLLAADGGERNADAEHAAAVDRHCAGTLH 77
Query: 88 PSLCVKSLLDFPGSTAASERDLVHISLNMTFQHLAKALYSSAAISAS-------MDPRVR 140
+CV +L P RD++ + A A+ +S++ S + R R
Sbjct: 78 RDVCVSTLSTIPNLARKPLRDVI----SEVVGRAASAVRASSSNCTSYLQRPRQLRTRDR 133
Query: 141 AAYDDCLELLDDSVDALGRSLSSFAAGSSSSD-----VMTWLSAALTNQDTCADGFADTS 195
A DCLEL ++D LG + + +AG+S+++ V T LSAA+TNQ TC DGFA S
Sbjct: 134 LALSDCLELFGHTLDLLGTAAAELSAGNSTAEESAAGVQTVLSAAMTNQYTCLDGFAGPS 193
Query: 196 ----GDVKDQMAGNLKDLSELVSNCLAIFAGAGDDFSGVPIQNRRLLAMRD--------D 243
G V+ + G + ++ LVSN LA+ +P Q RR
Sbjct: 194 ASEDGRVRPFIQGRIYHVAHLVSNSLAMVRR-------LPTQRRRGAEEEPLEGYGRVRR 246
Query: 244 NFPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAG 303
FP W++ DRR L V+A DVVV+KDG+G T++EA+ P R++IY++AG
Sbjct: 247 GFPSWVSASDRRRLQQQVAA---DVVVAKDGSGKFTTVSEAVAAAPNNSETRYVIYIKAG 303
Query: 304 RYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMT 363
Y EN +++G ++TN+M +GDG KTVI +NV+D TTF +A+ A G GF+ARD+T
Sbjct: 304 GYFEN-VEVGSEKTNIMFVGDGTWKTVIKASRNVVDNS-TTFRSATLAVVGTGFLARDIT 361
Query: 364 FENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGN 423
EN AGP KHQAVALRV AD + YRC+ GYQDT+Y HS RQFYR+CDIYGTVDFIFG+
Sbjct: 362 VENAAGPSKHQAVALRVNADLSAFYRCSFAGYQDTLYAHSLRQFYRDCDIYGTVDFIFGD 421
Query: 424 AAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETY 483
AAVV QNC+LYAR+P QKN TAQ R+DPNQNTGI+I C+V AAADL +F +Y
Sbjct: 422 AAVVLQNCNLYARRPDPNQKNVFTAQGREDPNQNTGIAIQGCKVAAAADLVPVQANFSSY 481
Query: 484 LGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWP 543
LGRPWK YSRTV++ S I +H RGWLEWN +FALDTLYY EYMN G GA RV+WP
Sbjct: 482 LGRPWKTYSRTVFLQSKIDSLIHPRGWLEWNGSFALDTLYYAEYMNRGDGADTSARVSWP 541
Query: 544 GYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 581
GY V+T+A +A+ FTV F+ G WL S+ ++ GLS
Sbjct: 542 GYHVLTNATDAANFTVLNFVQGDLWLNSSSFPYILGLS 579
>dbj|BAD53265.1| putative pectin esterase [Oryza sativa (japonica cultivar-group)]
Length = 546
Score = 459 bits (1180), Expect = e-127
Identities = 253/525 (48%), Positives = 324/525 (61%), Gaps = 25/525 (4%)
Query: 65 SGASTLRAKPTQAISNTCSKTRFPSLCVKSLLD--------FPGSTAASERDLVHISLNM 116
+GA+ L PT +N C+ + P+ C + D P A R ++ SL+
Sbjct: 38 AGAAFLLLSPTA--TNLCASSPDPASCQAIVADAVLASPHSHPSRPAHVLRAILATSLD- 94
Query: 117 TFQHLAKALYSSAAISASMDPRVRAAYDDCLELLDDSVDALGRSLSSFAAGSSSSDVMTW 176
+H A A + + DPR RAA +DC++L+ + D L + + D TW
Sbjct: 95 --RHDAAAEAVAGMRRRASDPRHRAALEDCVQLMGLARDRLADAAGAPDVDVDVDDARTW 152
Query: 177 LSAALTNQDTCADGFADTSGDVKDQMAGNLKDLSELVSNCLAIFAGAGDDFSGVPIQNRR 236
LSA LT+ TC DG D G ++D + +L+ L L S LA+ + AG R
Sbjct: 153 LSAVLTDHVTCLDGLDD--GPLRDSVGAHLEPLKSLASASLAVLSAAGRGA-------RD 203
Query: 237 LLAMRDDNFPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRF 296
+LA D FP WL RDR LL A+QADVVV+KDG+G TI EA+ P+ G R+
Sbjct: 204 VLAEAVDRFPSWLTARDRTLLDAGAGAVQADVVVAKDGSGKYTTIKEAVDAAPDGGKSRY 263
Query: 297 IIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAG 356
+IYV+ G Y+EN L++G+ + +MI+GDG +TVITG +NV+DG TTF++A+ A SG G
Sbjct: 264 VIYVKKGVYKEN-LEVGKTKRVLMIVGDGMDQTVITGSRNVVDGS-TTFNSATLALSGDG 321
Query: 357 FIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGT 416
I +D+ EN AG EK QAVALRV AD AV+ RC + GYQDT+Y H RQFYR+C + GT
Sbjct: 322 IILQDLKVENTAGAEKQQAVALRVSADRAVINRCRLDGYQDTLYAHQLRQFYRDCAVSGT 381
Query: 417 VDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKAS 476
VDF+FGNAA V Q C L AR+P QKN +TAQ R DPNQNTG SIH CRV+ A DL +
Sbjct: 382 VDFVFGNAAAVLQGCVLTARRPAQAQKNAVTAQGRTDPNQNTGTSIHRCRVVPAPDLAPA 441
Query: 477 NESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWN-TTFALDTLYYGEYMNYGPGAA 535
+ F T+LGRPWK YSRTVYM+SY+ HV RGWLEWN FAL TL+YGEY N GPGA+
Sbjct: 442 AKQFPTFLGRPWKEYSRTVYMLSYLDSHVDPRGWLEWNGADFALKTLFYGEYQNQGPGAS 501
Query: 536 VGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
RVNWPGY VIT A +FTV QFI G WL +TGV + GL
Sbjct: 502 TAGRVNWPGYHVITDQSVAMQFTVGQFIQGGNWLKATGVNYNEGL 546
>gb|AAK84486.1| putative thermostable pectinesterase [Citrus sinensis]
gi|24250746|gb|AAK84485.1| putative thermostable
pectinesterase [Citrus sinensis]
Length = 631
Score = 458 bits (1179), Expect = e-127
Identities = 253/585 (43%), Positives = 358/585 (60%), Gaps = 32/585 (5%)
Query: 23 VNSRTKTTSGRKKKLIILVLLAVVLIVASAVVLTAVRSRAGGSG-----ASTLRAKPTQA 77
++++ TT + +KL L L AVVL+V + V + A R + A+ A
Sbjct: 52 LSTKIFTTMAQPRKLF-LALFAVVLVVTAVVTIIATRKNNSSNTNDEHIAAHAHAYAQPV 110
Query: 78 ISNTCSKTRFPSLCVKSLLDFPGSTAA---SERDLVHISLNMTFQHLAKALYSSAAI--- 131
I ++CS T +P LC +L P + + S +D++ +SLN+T + ++ +
Sbjct: 111 IRSSCSATLYPELCFSALSAAPAAAVSKVNSPKDVIRLSLNLTITAVQHNYFAIKKLITT 170
Query: 132 -SASMDPRVRAAYDDCLELLDDSVDALGRS---LSSFAAGSSSSDVMTW-------LSAA 180
+++ R + + DCLE++D+++D L ++ L + A +++ + +SAA
Sbjct: 171 RKSTLTKREKTSLHDCLEMVDETLDELYKTEHELQGYPAAANNKSIAEQADELKILVSAA 230
Query: 181 LTNQDTCADGFADTSGD--VKDQMAGNLKDLSELVSNCLAIFAGAGDDFSGVPIQNRRLL 238
+TNQ+TC DGF+ D +++++ + + SN LA+ D G I +
Sbjct: 231 MTNQETCLDGFSHERADKKIREELMEGQMHVFHMCSNALAMIKNMTDGDIGKDIVDHYSK 290
Query: 239 AMRDDN---FPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRR 295
A R D+ +P WL+ DRRLL + + DV V+ DG+G T+A A+ PE SRR
Sbjct: 291 ARRLDDETKWPEWLSAGDRRLLQ--ATTVVPDVTVAADGSGNYLTVAAAVAAAPEGSSRR 348
Query: 296 FIIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGA 355
+II ++AG Y EN +++ +K+ N+M IGDG+ T+ITG +NV+DG TTF++A+ A G
Sbjct: 349 YIIRIKAGEYREN-VEVPKKKINLMFIGDGRTTTIITGSRNVVDGS-TTFNSATVAVVGD 406
Query: 356 GFIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYG 415
GF+ARD+TF+N AGP KHQAVALRVG+D + YRC+++ YQDT+YVHS RQFY C I G
Sbjct: 407 GFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAG 466
Query: 416 TVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKA 475
TVDFIFGNAA VFQNC ++AR+P Q+N +TAQ R DPNQNTGI I CR+ A +DL A
Sbjct: 467 TVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLA 526
Query: 476 SNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAA 535
SFETYLGRPWK YSRTV M S I D ++ GW EW+ FALDTL+Y EY N G GA
Sbjct: 527 VKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDTLFYAEYQNTGAGAD 586
Query: 536 VGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
RV W ++VITSA EA +T A FI G+TWL STG F GL
Sbjct: 587 TSNRVKWSTFKVITSAAEAQTYTAANFIAGSTWLGSTGFPFSLGL 631
>gb|AAO85706.1| pectin methyl-esterase [Nicotiana benthamiana]
Length = 579
Score = 456 bits (1172), Expect = e-126
Identities = 249/575 (43%), Positives = 352/575 (60%), Gaps = 37/575 (6%)
Query: 34 KKKLIILVLLAVVLIVASAVVLTAVRSRAGGSG----ASTLRAKPTQAISNTCSKTRFPS 89
KKKL + V+ +V+L+ A V+ V+ R+ S + + + + C T P
Sbjct: 14 KKKLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAITSAAHAIVKSACENTLHPE 73
Query: 90 LC---VKSLLDFPGSTAASERDLVHISLNMT---FQHLAKALYSSAAISASMDPRVRAAY 143
LC + S+ DF S++D++ +SLN+T QH + + PR + A
Sbjct: 74 LCYSTIASVSDF-SKKVTSQKDVIELSLNITCRAVQHNFFKVEKLIKTRKGLKPREKVAL 132
Query: 144 DDCLELLDDSVDALGRSLSSF-------AAGSSSSDVMTWLSAALTNQDTCADGFADTSG 196
DCLE +D+++D L ++ + + + + T +S+A+TNQ+TC DGF+
Sbjct: 133 HDCLETIDETLDELHTAIKDLELYPNKKSLKAHADGLKTLISSAITNQETCLDGFSHDDA 192
Query: 197 D--VKDQMAGNLKDLSELVSNCLAIFAGAGDDFSGVPIQNRRLLA-------MRDDN--F 245
D V+ + K + ++ SN LA+ D I N + L +R+DN +
Sbjct: 193 DKKVRKALLKGQKHVEKMCSNALAMICNMTD----TDIANEQKLKGTTTNRKLREDNSEW 248
Query: 246 PRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGRY 305
P WL DRRLL S ++ DVVV+ DG+G KT++EA+ K PE S+R++I ++AG Y
Sbjct: 249 PEWLPAGDRRLLQS--STVRPDVVVAADGSGNFKTVSEAVAKAPEKSSKRYVIRIKAGVY 306
Query: 306 EENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTFE 365
EN + + +K+TN+M +GDG+ T+ITG +NV DG TTFH+A+ AA G F+ARD+TF+
Sbjct: 307 REN-VDVPKKKTNIMFMGDGRSNTIITGSRNVKDGS-TTFHSATVAAVGEKFLARDITFQ 364
Query: 366 NYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAA 425
N AG KHQAVALRVG+D + YRC+++ YQD++YVHSNRQ++ +C I GTVDFIFGNAA
Sbjct: 365 NTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIAGTVDFIFGNAA 424
Query: 426 VVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETYLG 485
V Q+C ++AR+P + QKN +TAQ R DPNQNTGI I CR+ A +DL+ +SF YLG
Sbjct: 425 AVLQDCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPVQKSFPMYLG 484
Query: 486 RPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPGY 545
RPWK YSRTV M S I D ++ GW EWN FAL+TL+YGEY N G GA RV W G+
Sbjct: 485 RPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGAGTSGRVKWKGF 544
Query: 546 RVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
+VITSA EA +T +FI G +WL STG F GL
Sbjct: 545 KVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 579
>gb|AAL02367.1| pectin methylesterase [Lycopersicon esculentum]
gi|1222552|gb|AAD09283.1| pectin methylesterase
[Lycopersicon esculentum] gi|7447374|pir||T07848
pectinesterase (EC 3.1.1.11) - tomato
gi|6093740|sp|Q43143|PMEU_LYCES Pectinesterase U1
precursor (Pectin methylesterase) (PE)
Length = 583
Score = 454 bits (1167), Expect = e-126
Identities = 244/574 (42%), Positives = 353/574 (60%), Gaps = 33/574 (5%)
Query: 33 RKKKLIILVLLAVVLIVASAVVLTAVRSRAGGSG----ASTLRAKPTQAISNTCSKTRFP 88
RKKK+ + ++ +V+L+ A V+ V+S + S + + + + CS T P
Sbjct: 17 RKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHP 76
Query: 89 SLCVKSLLDFP--GSTAASERDLVHISLNMTFQHLAKALYSSAAISAS---MDPRVRAAY 143
LC ++++ S++D++ +SLN+T + + + Y+ + + + PR + A
Sbjct: 77 ELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVAL 136
Query: 144 DDCLELLDDSVDALGRSLSSFAAGSSSS-------DVMTWLSAALTNQDTCADGFADTSG 196
DCLE +D+++D L ++ + D+ T +S+A+TNQ+TC DGF+
Sbjct: 137 HDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDEA 196
Query: 197 D--VKDQMAGNLKDLSELVSNCLAIFAGAGD-DFSG-----VPIQNRRLLAMRDDN--FP 246
D V+ + K + ++ SN LA+ D D + P NR+L+ +DN +P
Sbjct: 197 DKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLV---EDNGEWP 253
Query: 247 RWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGRYE 306
WL+ DRRLL S + DVVV+ DG+G KT++EA++K PE S+R++I ++AG Y
Sbjct: 254 EWLSAGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVYR 311
Query: 307 ENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTFEN 366
EN + + +K+TN+M +GDGK T+IT +NV DG TTFH+A+ +ARD+TF+N
Sbjct: 312 EN-VDVPKKKTNIMFMGDGKSNTIITASRNVQDGS-TTFHSATVVRVAGKVLARDITFQN 369
Query: 367 YAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAV 426
AG KHQAVAL VG+D + YRC+++ YQDT+YVHSNRQF+ +C + GTVDFIFGN A
Sbjct: 370 TAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAA 429
Query: 427 VFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETYLGR 486
VFQ+C ++AR+P + QKN +TAQ R DPNQNTGI I CR+ A +DL+ +SF TYLGR
Sbjct: 430 VFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGR 489
Query: 487 PWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPGYR 546
PWK YSRTV M S I D + GW EWN FALDTL+YGEY N G GA RV W G++
Sbjct: 490 PWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHK 549
Query: 547 VITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
VITS+ EA +T +FI G +WL STG F GL
Sbjct: 550 VITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
>gb|AAN28889.1| At3g14310/MLN21_9 [Arabidopsis thaliana] gi|9279579|dbj|BAB01037.1|
pectinesterase [Arabidopsis thaliana]
gi|15529256|gb|AAK97722.1| AT3g14310/MLN21_9
[Arabidopsis thaliana] gi|14335010|gb|AAK59769.1|
AT3g14310/MLN21_9 [Arabidopsis thaliana]
gi|15231828|ref|NP_188048.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 592
Score = 453 bits (1166), Expect = e-126
Identities = 243/589 (41%), Positives = 353/589 (59%), Gaps = 41/589 (6%)
Query: 27 TKTTSGRKKKLIILVLLAVVLIVASAVVLTAVRSRAGGSGASTLRAKPTQAISNTCSKTR 86
+K + KKL++L +L VA+ ++A S+A TL + ++CS TR
Sbjct: 10 SKDNFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEK--RTLSPSSHAVLRSSCSSTR 67
Query: 87 FPSLCVKSLLDFPGSTAASERDLVHISLNMTFQHLAKALYSSAAI---SASMDPRVRAAY 143
+P LC+ +++ G S++D++ S+N+T + ++ + + PR + A
Sbjct: 68 YPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTAL 127
Query: 144 DDCLELLDDSVDALGRSLSSF-------AAGSSSSDVMTWLSAALTNQDTCADGFADTSG 196
DCLE +D+++D L ++ + D+ T +S+A+TNQ+TC DGF+
Sbjct: 128 HDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDA 187
Query: 197 D--VKDQMAGNLKDLSELVSNCLAIFAGAGD-------DFSGVPIQNRRLLA-------- 239
D V+ + + + SN LA+ D + + NR+L
Sbjct: 188 DKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVA 247
Query: 240 --------MRDDNFPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEY 291
+ + +P WL+ DRRLL S ++AD V+ DG+GT KT+A A+ PE
Sbjct: 248 VDIAGAGELDSEGWPTWLSAGDRRLLQ--GSGVKADATVAADGSGTFKTVAAAVAAAPEN 305
Query: 292 GSRRFIIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFA 351
++R++I+++AG Y EN +++ +K+ N+M +GDG+ +T+ITG +NV+DG TTFH+A+ A
Sbjct: 306 SNKRYVIHIKAGVYREN-VEVAKKKKNIMFMGDGRTRTIITGSRNVVDGS-TTFHSATVA 363
Query: 352 ASGAGFIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYREC 411
A G F+ARD+TF+N AGP KHQAVALRVG+D + Y C+++ YQDT+YVHSNRQF+ +C
Sbjct: 364 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 423
Query: 412 DIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAA 471
I GTVDFIFGNAAVV Q+C ++AR+P + QKN +TAQ R DPNQNTGI I CR+ A +
Sbjct: 424 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 483
Query: 472 DLKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYG 531
DL++ SF TYLGRPWK YS+TV M S I D + GW EW TFAL+TL Y EY N G
Sbjct: 484 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 543
Query: 532 PGAAVGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
GA RV W G++VIT+A EA ++T QFI G WL STG F GL
Sbjct: 544 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
>gb|AAC72288.1| putative pectin methylesterase [Arabidopsis thaliana]
Length = 592
Score = 449 bits (1156), Expect = e-125
Identities = 241/589 (40%), Positives = 351/589 (58%), Gaps = 41/589 (6%)
Query: 27 TKTTSGRKKKLIILVLLAVVLIVASAVVLTAVRSRAGGSGASTLRAKPTQAISNTCSKTR 86
+K + KKL++L +L VA+ ++A S+A TL + ++CS TR
Sbjct: 10 SKDNFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEK--RTLSPSSHAVLRSSCSSTR 67
Query: 87 FPSLCVKSLLDFPGSTAASERDLVHISLNMTFQHLAKALYSSAAI---SASMDPRVRAAY 143
+P LC+ +++ S++D++ S+N+T + ++ + + PR + A
Sbjct: 68 YPELCISAVVTAGACELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTAL 127
Query: 144 DDCLELLDDSVDALGRSLSSF-------AAGSSSSDVMTWLSAALTNQDTCADGFADTSG 196
DCLE +D+++D L ++ + D+ T +S+A+TNQ+TC DGF+
Sbjct: 128 HDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDA 187
Query: 197 D--VKDQMAGNLKDLSELVSNCLAIFAGAGD-------DFSGVPIQNRRLLA-------- 239
D V+ + + + SN LA+ D + + NR+L
Sbjct: 188 DKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVA 247
Query: 240 --------MRDDNFPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEY 291
+ + +P WL+ DRRLL S ++ D V+ DG+GT KT+A A+ PE
Sbjct: 248 VDIAGAGELDSEGWPTWLSAGDRRLLQ--GSGVKRDATVAADGSGTFKTVAAAVAAAPEN 305
Query: 292 GSRRFIIYVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFA 351
++R++I+++AG Y EN +++ +K+ N+M +GDG+ +T+ITG +NV+DG TTFH+A+ A
Sbjct: 306 SNKRYVIHIKAGVYREN-VEVAKKKKNIMFMGDGRTRTIITGSRNVVDGS-TTFHSATVA 363
Query: 352 ASGAGFIARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYREC 411
A G F+ARD+TF+N AGP KHQAVALRVG+D + Y C+++ YQDT+YVHSNRQF+ +C
Sbjct: 364 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 423
Query: 412 DIYGTVDFIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAA 471
I GTVDFIFGNAAVV Q+C ++AR+P + QKN +TAQ R DPNQNTGI I CR+ A +
Sbjct: 424 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 483
Query: 472 DLKASNESFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYG 531
DL++ SF TYLGRPWK YS+TV M S I D + GW EW TFAL+TL Y EY N G
Sbjct: 484 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 543
Query: 532 PGAAVGQRVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
GA RV W G++VIT+A EA ++T QFI G WL STG F GL
Sbjct: 544 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
>emb|CAE76633.2| pectin methylesterase [Cicer arietinum]
Length = 584
Score = 448 bits (1152), Expect = e-124
Identities = 252/582 (43%), Positives = 356/582 (60%), Gaps = 30/582 (5%)
Query: 25 SRTKTTSGRKKKLIILVLLAVVL----IVASAVVLTAVRSRAGG---SGASTLRAKPTQA 77
S T+ ++ KKKL + A+++ I SA V ++ +++ S + +L
Sbjct: 7 SLTRISNSNKKKLFFSLFTALLITASIIAISASVTSSQKTKTKNNIVSSSLSLSHHSHTI 66
Query: 78 ISNTCSKTRFPSLCVKSLLDFPGSTAA--SERDLVHISLNMTFQHLAKALYSSAAI--SA 133
I C+ T +P LC ++ P T + +D++ +SLN+T + + ++ +
Sbjct: 67 IKTACTTTLYPDLCFSAISSEPNITHKINNHKDVISLSLNITTRAVEHNFFTVENLLRRK 126
Query: 134 SMDPRVRAAYDDCLELLDDSVDALGRSLSSFAAGSS-------SSDVMTWLSAALTNQDT 186
++ R + A DCLE +DD++D L + + + D+ T +SAA+TNQ T
Sbjct: 127 NLSEREKIALHDCLETIDDTLDELKEAQRDLVLYPNKKTLYQHADDLKTLISAAITNQVT 186
Query: 187 CADGFADTSGD--VKDQMAGNLKDLSELVSNCLAIFAGAGD-DFSGVPI----QNRRLLA 239
C DGF+ D V+ + + + SN LA+ D D + +NR+LL
Sbjct: 187 CLDGFSHDGADKQVRKVLEQGQVHVEHMCSNALAMTKNMTDKDIAKFEENNNKKNRKLLE 246
Query: 240 MRDD-NFPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFII 298
+ N+P W++ DRRLL +A++ADVVV+ DG+G KT++EA+ P S+R++I
Sbjct: 247 EENGVNWPEWISAGDRRLLQ--GAAVKADVVVAADGSGNFKTVSEAVAGAPLKSSKRYVI 304
Query: 299 YVRAGRYEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFI 358
++AG Y+EN +++ +K++N+M +GDGK T+IT +NV+DG TTFH+A+ A G F+
Sbjct: 305 KIKAGVYKEN-VEVPKKKSNIMFLGDGKKNTIITASRNVVDGS-TTFHSATVAVVGGNFL 362
Query: 359 ARDMTFENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVD 418
ARD+TF+N AGP KHQAVALRVG D + Y C++I YQDT+YVH+NRQF+ C I GTVD
Sbjct: 363 ARDITFQNTAGPSKHQAVALRVGGDLSAFYNCDIIAYQDTLYVHNNRQFFVNCFISGTVD 422
Query: 419 FIFGNAAVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNE 478
FIFGN+AVVFQNC ++ARKP + QKN +TAQ R DPNQNTGI I CR+ A DL+
Sbjct: 423 FIFGNSAVVFQNCDIHARKPDSGQKNMVTAQGRVDPNQNTGIVIQKCRIGATKDLEGLKG 482
Query: 479 SFETYLGRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQ 538
+F TYLGRPWK YSRTV M S I D + GW EWN FAL+TL Y EY N GPGA +
Sbjct: 483 TFPTYLGRPWKEYSRTVIMQSSISDVIDPIGWHEWNGNFALNTLVYREYQNTGPGAGTSK 542
Query: 539 RVNWPGYRVITSALEASRFTVAQFILGATWLPSTGVAFLSGL 580
RVNW G++VITSA EA FT FI G+TWL STG F GL
Sbjct: 543 RVNWKGFKVITSASEAQTFTPGNFIGGSTWLGSTGFPFSLGL 584
>gb|AAK81875.1| pectin methylesterase PME1 [Vitis vinifera]
Length = 531
Score = 447 bits (1151), Expect = e-124
Identities = 230/457 (50%), Positives = 301/457 (65%), Gaps = 19/457 (4%)
Query: 136 DPRVRAAYDDCLELLDDSVDALGRSLSSF--------AAGSSSSDVMTWLSAALTNQDTC 187
D R+ A DCLELLD + D L SLS+ G SD+ TWLS+ TNQDTC
Sbjct: 82 DFRLSNAISDCLELLDFAADDLSWSLSAIQNPKGKDNGTGDLGSDLKTWLSSTFTNQDTC 141
Query: 188 ADGFADTSGDVKDQMAGNLKDLSELVSNCLAIF---AGAGDDFSGVPIQNRRLLAMRDDN 244
+GF T+G VK +A +L ++ LV + L + A G G + + +
Sbjct: 142 IEGFVGTNGIVKTVVAESLSQVASLVHSLLTMVHDPAPKGKSNGG----GGGVKHVGSGD 197
Query: 245 FPRWLNRRDRRLLSLPVSAMQADVVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGR 304
FP W+ + R+LL S++ DV V+ DG G T+ +A++ P+Y ++IY++ G
Sbjct: 198 FPSWVGKHSRKLLQ--ASSVSPDVTVAADGTGNYTTVMDAVQAAPDYSQNHYVIYIKQGI 255
Query: 305 YEENNLKIGRKRTNVMIIGDGKGKTVITGGKNVMDGLLTTFHTASFAASGAGFIARDMTF 364
Y EN ++I +K+ N+M++GDG G TVITG ++ +DG TT+ +A+FA G GFIARDMTF
Sbjct: 256 YREN-VEIKKKKWNLMMVGDGMGATVITGNRSYIDGW-TTYASATFAVKGKGFIARDMTF 313
Query: 365 ENYAGPEKHQAVALRVGADHAVVYRCNVIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNA 424
EN AGPEKHQAVALR +D +V YRC++ GYQDT+Y H+NRQFYREC I GTVDFIFG+A
Sbjct: 314 ENTAGPEKHQAVALRSDSDLSVYYRCSMRGYQDTLYPHTNRQFYRECRISGTVDFIFGDA 373
Query: 425 AVVFQNCSLYARKPMAQQKNTITAQNRKDPNQNTGISIHACRVLAAADLKASNESFETYL 484
VVFQNC + +K + QKNTITAQ RKDP Q TG SI + A +DL AS S +YL
Sbjct: 374 TVVFQNCQILVKKGLPNQKNTITAQGRKDPAQPTGFSIQFSNISADSDLLASVNSTLSYL 433
Query: 485 GRPWKLYSRTVYMMSYIGDHVHQRGWLEWNTTFALDTLYYGEYMNYGPGAAVGQRVNWPG 544
GRPWK YSRT+ M SYI D + GWLEWN FALDTLYYGEYMNYGP A +G RV WPG
Sbjct: 434 GRPWKQYSRTIIMKSYISDAIRPEGWLEWNGDFALDTLYYGEYMNYGPSAGLGSRVQWPG 493
Query: 545 YRVITSALEASRFTVAQFILGATWLPSTGVAFLSGLS 581
+ ++ ++ +A+ FTV +FI G WLPSTGV + +GL+
Sbjct: 494 FHLLNNSAQAANFTVTEFIAGNLWLPSTGVKYSAGLA 530
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.319 0.133 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 950,718,186
Number of Sequences: 2540612
Number of extensions: 39008163
Number of successful extensions: 93691
Number of sequences better than 10.0: 433
Number of HSP's better than 10.0 without gapping: 345
Number of HSP's successfully gapped in prelim test: 88
Number of HSP's that attempted gapping in prelim test: 92121
Number of HSP's gapped (non-prelim): 494
length of query: 582
length of database: 863,360,394
effective HSP length: 134
effective length of query: 448
effective length of database: 522,918,386
effective search space: 234267436928
effective search space used: 234267436928
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 78 (34.7 bits)
Lotus: description of TM0039.5