BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0588b.2
(157 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g16195 putative protein 107 3e-24
At3g16970 S1 self-incompatibility protein, putative 103 3e-23
At3g17080 S1 self-incompatibility protein, putative 95 1e-20
At5g12060 putative protein 88 2e-18
At1g04645 unknown protein 87 4e-18
At5g12070 unknown protein 87 5e-18
At5g06030 unknown protein 60 6e-10
At1g26795 unknown protein 59 1e-09
At5g06020 unknown protein 57 5e-09
At2g06090 putative S1 self-incompatibility protein 50 7e-07
At3g57840 hypothetical protein 47 3e-06
At4g16295 putative protein 47 4e-06
At3g24060 hypothetical protein 47 4e-06
At3g16960 hypothetical protein 47 6e-06
At3g26880 putative self-incompatibility protein 46 7e-06
At5g04350 putative protein 42 2e-04
At5g38440 contains similarity to S1 self-incompatibility protein 35 0.017
At3g10460 hypothetical protein 35 0.022
At5g11820 putative protein 34 0.028
At5g06040 unknown protein 28 2.0
>At4g16195 putative protein
Length = 161
Score = 107 bits (267), Expect = 3e-24
Identities = 60/134 (44%), Positives = 79/134 (58%), Gaps = 16/134 (11%)
Query: 22 DTGVTFSIFPP-KVTVEIINDLPQPPSTIPLTFHCKSKDDDLGIHTLNHGEVFSFHFKPS 80
D T S+FP K TVEI NDL + + L +HCKSKDDDLG TL GE +SF F
Sbjct: 35 DPSSTNSVFPTSKRTVEINNDLG---NQLTLLYHCKSKDDDLGNRTLQPGESWSFSFGRQ 91
Query: 81 IIRFSSTLFFCSFTWPDKTPSRHYFDVYDQDRDS-----CKF--CSWKMSKTGGCMYEEK 133
F TL+FCSF+WP+++ H FD+Y RDS C+ C WK+ + G C + ++
Sbjct: 92 F--FGRTLYFCSFSWPNES---HSFDIYKDHRDSGGDNKCESDRCVWKIRRNGPCRFNDE 146
Query: 134 TGRHDICVPWNNEL 147
T + D+C PWN L
Sbjct: 147 TKQFDLCYPWNKSL 160
>At3g16970 S1 self-incompatibility protein, putative
Length = 140
Score = 103 bits (258), Expect = 3e-23
Identities = 50/121 (41%), Positives = 69/121 (56%), Gaps = 14/121 (11%)
Query: 32 PKVTVEIINDLPQPPSTIPLTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFC 91
P+ TV I NDL +PL +HCKSK+DDLG + +SF F+PS+ F TLFFC
Sbjct: 26 PRTTVVIYNDLG---GHLPLRYHCKSKNDDLGDRNMAVNGTWSFEFRPSV--FGGTLFFC 80
Query: 92 SFTWPDKTPSRHYFDVYDQDRD------SCKFCSWKMSKTGGCMYEEKTGRHDICVPWNN 145
F W + H+FD+Y Q RD C+ C WK+ K G C + + D+C+PWN+
Sbjct: 81 GFIWDKEL---HWFDIYKQSRDREFAEFGCRRCEWKIRKDGPCKLNKNSNMFDVCLPWNS 137
Query: 146 E 146
+
Sbjct: 138 Q 138
>At3g17080 S1 self-incompatibility protein, putative
Length = 134
Score = 95.1 bits (235), Expect = 1e-20
Identities = 54/142 (38%), Positives = 74/142 (52%), Gaps = 21/142 (14%)
Query: 9 LLLFMTILFALRFDTGVTFSIFPPKVTVEIINDLPQPPSTIPLTFHCKSKDDDLGIHTLN 68
LL F+ ++F + + S+ I NDL +PL HCKS++DDLG +L
Sbjct: 8 LLFFVMVMFMSAIMSRASTSVV-------IYNDLG---GGLPLRHHCKSREDDLGYQSLA 57
Query: 69 HGEVFSFHFKPSIIRFSSTLFFCSFTWPDKTPSRHYFDVYDQDRD------SCKFCSWKM 122
G +SF F P I F TLF+C F+W ++ H FD+Y Q RD CK C WK+
Sbjct: 58 PGRSWSFGFTPDI--FGRTLFYCRFSWGAES---HIFDIYKQSRDKEFQEFGCKKCEWKI 112
Query: 123 SKTGGCMYEEKTGRHDICVPWN 144
K G C + +KTG D C W+
Sbjct: 113 RKNGPCKFYKKTGMFDHCYSWD 134
>At5g12060 putative protein
Length = 150
Score = 88.2 bits (217), Expect = 2e-18
Identities = 45/105 (42%), Positives = 58/105 (54%), Gaps = 11/105 (10%)
Query: 49 IPLTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFCSFTWPDKTPSRHYFDVY 108
+ L HCKS DDDLG+ L +SF F+ SI+ +TLF+C FTWP ++ FD+Y
Sbjct: 49 LTLNLHCKSADDDLGLKILAPNGSWSFKFRTSIV--GTTLFYCHFTWPGQSKR---FDIY 103
Query: 109 DQDRD------SCKFCSWKMSKTGGCMYEEKTGRHDICVPWNNEL 147
D DRD SC C W +S G CM+ E +IC WN L
Sbjct: 104 DDDRDGVRSHISCINCIWDISIQGPCMFSESDHAFNICYDWNGNL 148
>At1g04645 unknown protein
Length = 128
Score = 87.0 bits (214), Expect = 4e-18
Identities = 55/141 (39%), Positives = 75/141 (53%), Gaps = 21/141 (14%)
Query: 7 VSLLLFMTILFALRFDTGVTFSIFPP--KVTVEIINDLPQPPSTIPLTFHCKSKDDDLGI 64
VS++ F F L F +G + PP + TV + +L P PLT HCKSK DDLGI
Sbjct: 4 VSIVCFF---FFLLFGSG--YGGLPPFWRATVVTMTNLIGGP---PLTIHCKSKQDDLGI 55
Query: 65 HTLNHGEVFSFHFKPSIIRFSSTLFFCSFTWPDKTPSRHYFDVYDQDRDS--CKFCSWKM 122
H + + + F F+P++ + STLFFCSF W + S FD+YD RD C C W++
Sbjct: 56 HVVPFKQEYHFKFQPNL--WKSTLFFCSFQWDSQFKS---FDIYDAQRDQGICDDCQWEI 110
Query: 123 SKTGGCMYEEKTGRHDICVPW 143
G C + G+ C PW
Sbjct: 111 KPDGPC----RLGKKAKCFPW 127
>At5g12070 unknown protein
Length = 149
Score = 86.7 bits (213), Expect = 5e-18
Identities = 43/100 (43%), Positives = 56/100 (56%), Gaps = 10/100 (10%)
Query: 51 LTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFCSFTWPDKTPSRHYFDVYDQ 110
L HCKS DDDLG+ L +SF F+PSI+ TLFFC FTWP ++ +F++YD
Sbjct: 49 LKLHCKSADDDLGLKILAPNGSWSFKFRPSIVP-GVTLFFCHFTWPGQS---KWFNIYDD 104
Query: 111 DRDS------CKFCSWKMSKTGGCMYEEKTGRHDICVPWN 144
DRD C +C W + K G C + E +IC WN
Sbjct: 105 DRDGVRMGIPCIYCIWDIGKYGPCRFSEIDDAFNICYDWN 144
>At5g06030 unknown protein
Length = 150
Score = 59.7 bits (143), Expect = 6e-10
Identities = 41/115 (35%), Positives = 57/115 (48%), Gaps = 13/115 (11%)
Query: 33 KVTVEIINDLPQPPSTIPLTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFCS 92
+VT++ ND L HCKS+DDDLG H L GE+F + F + R+ STL FC
Sbjct: 47 RVTIQNYNDYL-------LAIHCKSRDDDLGFHILAKGELFGWKFHVN-FRY-STLCFCG 97
Query: 93 FTWPDKTPSRHYFDVYDQDRD--SCKFCSWKMSKTGGCMYEEKTGRHDICVPWNN 145
F+ + ++ F +Y RD C C+WK K G Y + R + W N
Sbjct: 98 FS--QRQINKGVFIIYVASRDFYRCANCTWKAEKDGFHGYGDIPTRGYLFYNWLN 150
>At1g26795 unknown protein
Length = 151
Score = 58.9 bits (141), Expect = 1e-09
Identities = 32/101 (31%), Positives = 49/101 (47%), Gaps = 14/101 (13%)
Query: 51 LTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFCSFTWPDKTPSRHYFDVYDQ 110
L HC +K +DLG+ LN + F F F+ ++ + +T + CSF WP T + FD++
Sbjct: 55 LIVHCTNKGEDLGVIRLNPLDRFDFRFRVNLRK--TTTYTCSFEWPGNTAT---FDIFRA 109
Query: 111 DRDS--------CKFCSWKMSKTGGCMYEEKTGRHDICVPW 143
DRD C C W + + C + + G C PW
Sbjct: 110 DRDDNPSGKYGVCSECIWSIYEPAPCR-DRRDGGQPQCFPW 149
>At5g06020 unknown protein
Length = 151
Score = 56.6 bits (135), Expect = 5e-09
Identities = 37/118 (31%), Positives = 59/118 (49%), Gaps = 12/118 (10%)
Query: 9 LLLFMTILFALRFDTGVTFSIFPPKVTVEIINDLPQPPSTIPLTFHCKSKDDDLGIHTLN 68
+++F+T + D G F I P + + N+ + L HCKSKDDDLG H
Sbjct: 23 VMIFVTCYETFQQD-GEPFPIRGPLTRITVKNN-----NDYLLGIHCKSKDDDLGFHIHK 76
Query: 69 HGEVFSFHFKPSIIRFSSTLFFCSFTWPDKTPSRHYFDVYDQDRD--SCKFCSWKMSK 124
GE++ + F + +STL+FC F+ ++ FD+ +RD C+ C+W K
Sbjct: 77 EGELYGWKFHVNF--QNSTLYFCGFS--QGQDNKGVFDIDRAERDFYRCRNCTWNAKK 130
>At2g06090 putative S1 self-incompatibility protein
Length = 135
Score = 49.7 bits (117), Expect = 7e-07
Identities = 23/42 (54%), Positives = 29/42 (68%), Gaps = 2/42 (4%)
Query: 51 LTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFCS 92
LT HCKSKDDDLGIHT+ ++F F+ S+ F T FFC+
Sbjct: 42 LTVHCKSKDDDLGIHTVARSYEYNFKFEDSV--FGRTEFFCT 81
>At3g57840 hypothetical protein
Length = 154
Score = 47.4 bits (111), Expect = 3e-06
Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 6/63 (9%)
Query: 87 TLFFCSFTWPDKTPSRHYFDVYDQDRDS-----CKFCSWKMSKTGGCMYEEKTGRHDICV 141
T + C F + D+T H F +Y RD+ C+ C W + + G C TG++DIC
Sbjct: 92 TRYHCEFRFGDETQI-HRFSLYKDIRDNIDKYQCRHCFWSIRRNGPCALNSHTGKYDICY 150
Query: 142 PWN 144
W+
Sbjct: 151 AWD 153
>At4g16295 putative protein
Length = 151
Score = 47.0 bits (110), Expect = 4e-06
Identities = 42/137 (30%), Positives = 64/137 (46%), Gaps = 22/137 (16%)
Query: 26 TFSIFPPKVT---VEIINDLPQPPSTIPLTFHCKSKDDDLGIHTLNHGEVFSFHFKPSII 82
T I PK++ V ++N L + L HCKSK+DDLG L FS++F +++
Sbjct: 30 TRDIIVPKISEWQVTVVNGLTTGET---LFIHCKSKEDDLGEINLKFRNRFSWNFGENML 86
Query: 83 RFSSTLFFCSFTWPDKTPSRHYFDVYDQDRDSCKFCSWKMSKTGGCMYEEKTGRHDICVP 142
ST F+C + +K +V+ D C WK C++ KT D
Sbjct: 87 H--STFFWC---YMNKDNGHMNVNVFWDDVILFHRCGWK-----NCIWTAKT---DGLYL 133
Query: 143 WNN---ELNLHKRWELG 156
WN+ E L ++WE+G
Sbjct: 134 WNSASGEDVLSRKWEVG 150
>At3g24060 hypothetical protein
Length = 147
Score = 47.0 bits (110), Expect = 4e-06
Identities = 39/134 (29%), Positives = 58/134 (43%), Gaps = 18/134 (13%)
Query: 4 QKMVSLLLFM------TILFALRFDTGVTFSIFPPKVTVEIINDLPQPPSTIPLTFHCKS 57
+K + LLLFM TI+F L F + V +IN + S++PL C S
Sbjct: 4 RKTIILLLFMLIAISLTIIFTLMLQPQTMF--LGEEFDVRVINSF-RDNSSLPLVIWCTS 60
Query: 58 KDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFCSFTWPDKTPSRHYFDVYDQDRD---- 113
DLG L G+ F + K + + + + C+ W K R F+ + RD
Sbjct: 61 PQGDLGGRALQEGDDFEWTAKIDLWSWMAE-YTCTMKWDSK---RKQFEAFKVSRDSNRC 116
Query: 114 -SCKFCSWKMSKTG 126
S K CSW + + G
Sbjct: 117 GSTKKCSWSVREDG 130
>At3g16960 hypothetical protein
Length = 114
Score = 46.6 bits (109), Expect = 6e-06
Identities = 31/123 (25%), Positives = 48/123 (38%), Gaps = 46/123 (37%)
Query: 28 SIFPPKVTVEIINDLPQPPSTIPLTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSST 87
S+ P TV I N L P+ +HC SKD D G+ +
Sbjct: 32 SVENPNTTVVIHNALS---GYWPMAYHCASKDVDFGLRRM-------------------- 68
Query: 88 LFFCSFTWPDKTPSRHYFDVYDQDRDS----CKFCSWKMSKTGGCMYEEKTGRHDICVPW 143
+ ++D++S CK C WK+ + G C +KTG D+C PW
Sbjct: 69 -------------------LAERDQESAMFGCKRCEWKIREEGPCKLNKKTGMFDLCPPW 109
Query: 144 NNE 146
+++
Sbjct: 110 DSQ 112
>At3g26880 putative self-incompatibility protein
Length = 133
Score = 46.2 bits (108), Expect = 7e-06
Identities = 31/94 (32%), Positives = 51/94 (53%), Gaps = 11/94 (11%)
Query: 5 KMVSLLLFMTILFALRFDTGVTFSIFPPKVTVEIINDLPQPPSTIPLTFHCKSKDDDLGI 64
K +S+ LF+ L + ++ K T+ + N+L P + L HCKSK++D+G+
Sbjct: 2 KNLSIFLFVVGLCMIS-------DVYGKKSTITVKNEL-NPKNKNILKVHCKSKNNDIGV 53
Query: 65 HTLNHGEVFSFHFKPSIIRFSSTLFFCS-FTWPD 97
L GEV SF FK + + +T F+C+ + PD
Sbjct: 54 KYLKIGEVMSFSFKTNF--WGTTEFWCNLYKGPD 85
>At5g04350 putative protein
Length = 146
Score = 41.6 bits (96), Expect = 2e-04
Identities = 29/89 (32%), Positives = 42/89 (46%), Gaps = 15/89 (16%)
Query: 51 LTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFCSFTWPDKTPS-RHYFDVYD 109
L HC+SKDDDLG H L G+ + F F +I + +T F C D+ P+ +H+ D
Sbjct: 41 LKVHCRSKDDDLGEHILKIGQDYEFTFGDNI--WQTTSFSCQM---DQGPNFKHHLDFVA 95
Query: 110 QDRDSCKFCSWKMSKTGGCMYEEKTGRHD 138
+ SW + C + GR D
Sbjct: 96 YE------TSWSKALEASCKW---IGRED 115
>At5g38440 contains similarity to S1 self-incompatibility protein
Length = 133
Score = 35.0 bits (79), Expect = 0.017
Identities = 18/41 (43%), Positives = 23/41 (55%), Gaps = 4/41 (9%)
Query: 51 LTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFC 91
L HC S +D+LG H L GE + F F SI+R F+C
Sbjct: 42 LKIHCTS-EDNLGFHFLRPGETYDFSFHDSIVRSD---FYC 78
>At3g10460 hypothetical protein
Length = 132
Score = 34.7 bits (78), Expect = 0.022
Identities = 25/79 (31%), Positives = 35/79 (43%), Gaps = 6/79 (7%)
Query: 35 TVEIINDLPQPPSTIPLTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIRFSSTLFFCSFT 94
+V I N+LP L CKS D +LG H G +++ F I+ TL++C
Sbjct: 23 SVFIYNNLPHGHY---LQVKCKSGDTNLGFHVRRPGRFYNYAFTDHIL--GKTLYWCHL- 76
Query: 95 WPDKTPSRHYFDVYDQDRD 113
W K H V + RD
Sbjct: 77 WKGKNWEDHVVIVAYESRD 95
>At5g11820 putative protein
Length = 175
Score = 34.3 bits (77), Expect = 0.028
Identities = 21/60 (35%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Query: 37 EIINDLP-QPPSTIPLTFHCKSKDDDLGIHTLNHGEVFSFHFKPSIIR--FSSTLFFCSF 93
E+ N++ P L +C S ++ LG H L G+ S+ FKP I+ F T F C+F
Sbjct: 38 EVRNEISIYPGRNRSLAINCWSSNNKLGTHALKPGQSKSWSFKPIFIKIPFFYTYFECTF 97
>At5g06040 unknown protein
Length = 111
Score = 28.1 bits (61), Expect = 2.0
Identities = 18/47 (38%), Positives = 21/47 (44%), Gaps = 7/47 (14%)
Query: 51 LTFHCKSKDDDLGIHTLNHGEVFSF----HFKPSI---IRFSSTLFF 90
L CK DDDLG H L + F H K + IR + TL F
Sbjct: 58 LGIQCKPGDDDLGFHILTKDLLLCFSSCSHVKQKMARRIRLTKTLIF 104
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.327 0.141 0.474
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,109,398
Number of Sequences: 26719
Number of extensions: 184228
Number of successful extensions: 489
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 448
Number of HSP's gapped (non-prelim): 24
length of query: 157
length of database: 11,318,596
effective HSP length: 91
effective length of query: 66
effective length of database: 8,887,167
effective search space: 586553022
effective search space used: 586553022
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0588b.2